scholarly journals Transcriptome provides potential insights into how calcium affects the formation of stone cell in Pyrus

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Xingyu Tao ◽  
Min Liu ◽  
Yazhou Yuan ◽  
Ruonan Liu ◽  
Kaijie Qi ◽  
...  
Keyword(s):  
Calcium Nitrate ◽  
Great Influence ◽  
Lignin Biosynthesis ◽  
Calcium Signal ◽  
Nitrate Treatment ◽  
Stone Cell ◽  
Lignin Synthesis ◽  
Secondary Metabolic Pathway ◽  
Exogenous Calcium

Abstract Background The content of stone cells in pears has a great influence on taste. Stone cells are formed by the accumulation of lignin. The treatment of exogenous calcium can affect the lignin synthesis, but this Ca-mediated mechanism is still unclear. In this study, the author performed a comparative transcriptomic analysis of callus of pears (Pyrus x bretschneideri) treated with calcium nitrate Ca (NO3)2 to investigate the role of calcium in lignin synthesis. Results There were 2889 differentially expressed genes (DEGs) detected between the Control and Ca (NO3)2 treatment in total. Among these 2889 DEGs, not only a large number of genes related to Ca single were found, but also many genes were enriched in secondary metabolic pathway, especially in lignin synthesis. Most of them were up-regulated during the development of callus after Ca (NO3)2 treatment. In order to further explore how calcium nitrate treatment affects lignin synthesis, the author screened genes associated with transduction of calcium signal in DEGs, and finally found CAM, CML, CDPK, CBL and CIPK. Then the author identified the PbCML3 in pears and conducted relevant experiments finding the overexpression of PbCML3 would increase the content of pear stone cells, providing potential insights into how Ca treatment enhances the stone cell in pears. Conclusions Our deep analysis reveals the effects of exogenous calcium on calcium signal and lignin biosynthesis pathway. The function of PbCML3 on stone cells formation was verified in pear.

scholarly journals Drought-induced ABA, H2O2 and JA positively regulate CmCAD genes and lignin synthesis in melon stems

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Wei Liu ◽  
Yun Jiang ◽  
Yazhong Jin ◽  
Chenghui Wang ◽  
Juan Yang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Expression Profiles ◽  
Lignin Biosynthesis ◽  
Cinnamyl Alcohol Dehydrogenase ◽  
Signal Molecules ◽  
Lignin Synthesis ◽  
Enzyme Functions ◽  
Stress Promoter ◽  
Important Enzyme

Abstract Background Cinnamyl alcohol dehydrogenase (CAD) is an important enzyme functions at the last step in lignin monomer synthesis pathway. Our previous work found that drought induced the expressions of CmCAD genes and promoted lignin biosynthesis in melon stems. Results Here we studied the effects of abscisic acid (ABA), hydrogen peroxide (H2O2) and jasmonic acid (JA) to CmCADs under drought stress. Results discovered that drought-induced ABA, H2O2 and MeJA were prevented efficiently from increasing in melon stems pretreated with fluridone (Flu, ABA inhibitor), imidazole (Imi, H2O2 scavenger) and ibuprofen (Ibu, JA inhibitor). ABA and H2O2 are involved in the positive regulations to CmCAD1, 2, 3, and 5, and JA is involved in the positive regulations to CmCAD2, 3, and 5. According to the expression profiles of lignin biosynthesis genes, ABA, H2O2 and MeJA all showed positive regulations to CmPAL2-like, CmPOD1-like, CmPOD2-like and CmLAC4-like. In addition, positive regulations were also observed with ABA to CmPAL1-like, CmC4H and CmCOMT, with H2O2 to CmPAL1-like, CmC4H, CmCCR and CmLAC17-like, and with JA to CmCCR, CmCOMT, CmLAC11-like and CmLAC17-like. As expected, the signal molecules positively regulated CAD activity and lignin biosynthesis under drought stress. Promoter::GUS assays not only further confirmed the regulations of the signal molecules to CmCAD1~3, but also revealed the important role of CmCAD3 in lignin synthesis due to the strongest staining of CmCAD3 promoter::GUS. Conclusions CmCADs but CmCAD4 are positively regulated by ABA, H2O2 and JA under drought stress and participate in lignin synthesis.

Tax evasion in the medical profession An Empirical Study in the General Tax Authority in Iraq

2019 ◽  
Vol 118 (11) ◽  
pp. 80-88
Author(s):  
Ramyar Rzgar Ahmed ◽  
Hawkar Qasim Birdawod ◽  
S. Rabiyathul Basariya
Keyword(s):  
Tax Evasion ◽  
Income Tax ◽  
Medical Profession ◽  
Great Influence ◽  
Tax Law ◽  
Degree Of Confidence ◽  
Medical Professions ◽  
Shed Light

The study dealt with tax evasion in the medical profession, where the problem was the existence of many cases of tax evasion, especially tax evasion in the income tax of medical professions. The aim of the study is to try to shed light on the phenomenon of tax evasion and the role of the tax authority in the development of controls and means that reduce the phenomenon of tax evasion. The most important results of the low level of tax awareness and lack of knowledge of the tax law and the unwillingness to read it and the sense of taxpayers unfairness of the tax all lead to an increase in cases of tax evasion and in suggested tightening control and follow-up on the offices of auditors, through the investigation and auditing The reports of certified accountants and the use of computers for this purpose in order to raise the degree of confidence in these reports and bring them closer to the required truth and coordination and cooperation with the Union of Accountants and Auditors and inform them about each case of violations of the auditors and accountants N because of its great influence in the rejection of the organization of the accounts and not to ratify fake accounts lead to show taxpayers accounts on a non-truth in order to tax evasion.

Role of Brand experience in Fashion brands

2019 ◽  
Vol 118 (1) ◽  
pp. 57-64
Author(s):  
G. Aiswarya ◽  
Dr. Jayasree Krishnan
Keyword(s):  
Factor Analysis ◽  
Purchase Intention ◽  
Great Influence ◽  
Brand Experience ◽  
Brand Preference ◽  
Mediating Role ◽  
Confirmatory Factor ◽  
Selling Strategies ◽  
The Impact

Traditionally the products were pushed into the hands of customers by production and selling strategies; then the marketing strategy evolved which gained momentum by understanding the customer needs and developing products satisfying those needs. This strategy is most prevalent and what should be done to stand up in this most competitive scenario? The answer to this key question is to create an experience. The customers now also seek good experiences than other benefits. Brand experience has gained more attention, especially fashion brands. Previous studies demonstrate the role of the brand experience in brand equity and other consumer behavior constructs. But very little is known about the impact of brand experiences on fashion brands. The aim of this study is to develop a model which makes our understanding better about the role of Brand preference and Brand experience and its influence on purchase intention of the brand. An initial exploratory study is conducted using a focus group to generate items for the study. The items, thus generated are prepared in the form of a questionnaire and samples were collected.  Exploratory factor analysis is conducted and the reliability of the constructs is determined. These constructs are loaded onto AMOS to perform Confirmatory factor analysis. The results confirmed the scales used. We also noticed that Brand preference has a great influence on the Brand experience. Thereby the finding supports the role of the brand experience which tends to have a mediating role in influencing the purchase intention.

scholarly journals Integrated transcriptomic and proteomic analysis reveals the complex molecular mechanisms underlying stone cell formation in Korla pear

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Aisajan Mamat ◽  
Kuerban Tusong ◽  
Juan Xu ◽  
Peng Yan ◽  
Chuang Mei ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Proteomic Analysis ◽  
Molecular Mechanisms ◽  
Developmental Stages ◽  
Cell Formation ◽  
Parenchyma Cell ◽  
Lignin Biosynthesis ◽  
Cell Content ◽  
Stone Cell ◽  
Fruit Samples

AbstractKorla pear (Pyrus sinkiangensis Yü) is a landrace selected from a hybrid pear species in the Xinjiang Autonomous Region in China. In recent years, pericarp roughening has been one of the major factors that adversely affects fruit quality. Compared with regular fruits, rough-skin fruits have a greater stone cell content. Stone cells compose sclerenchyma tissue that is formed by secondary thickening of parenchyma cell walls. In this work, we determined the main components of stone cells by isolating them from the pulp of rough-skin fruits at the ripening stage. Stone cell staining and apoptosis detection were then performed on fruit samples that were collected at three different developmental stages (20, 50 and 80 days after flowering (DAF)) representing the prime, late and stationary stages of stone cell differentiation, respectively. The same batches of samples were used for parallel transcriptomic and proteomic analysis to identify candidate genes and proteins that are related to SCW biogenesis in Korla pear fruits. The results showed that stone cells are mainly composed of cellulose (52%), hemicellulose (23%), lignin (20%) and a small amount of polysaccharides (3%). The periods of stone cell differentiation and cell apoptosis were synchronous and primarily occurred from 0 to 50 DAF. The stone cell components increased abundantly at 20 DAF but then decreased gradually. A total of 24,268 differentially expressed genes (DEGs) and 1011 differentially accumulated proteins (DAPs) were identified from the transcriptomic and proteomic data, respectively. We screened the DEGs and DAPs that were enriched in SCW-related pathways, including those associated with lignin biosynthesis (94 DEGs and 31 DAPs), cellulose and xylan biosynthesis (46 DEGs and 18 DAPs), S-adenosylmethionine (SAM) metabolic processes (10 DEGs and 3 DAPs), apoplastic ROS production (16 DEGs and 2 DAPs), and cell death (14 DEGs and 6 DAPs). Among the identified DEGs and DAPs, 63 significantly changed at both the transcript and protein levels during the experimental periods. In addition, the majority of these identified genes and proteins were expressed the most at the prime stage of stone cell differentiation, but their levels gradually decreased at the later stages.

scholarly journals Genetic Suppressor Element 1 (GSE1) Promotes the Oncogenic and Recurrent Phenotypes of Castration-Resistant Prostate Cancer by Targeting Tumor-Associated Calcium Signal Transducer 2 (TACSTD2)

Cancers ◽  
2021 ◽  
Vol 13 (16) ◽  
pp. 3959
Author(s):  
Oluwaseun Adebayo Bamodu ◽  
Yuan-Hung Wang ◽  
Chen-Hsun Ho ◽  
Su-Wei Hu ◽  
Chia-Da Lin ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Disease Progression ◽  
Therapy Resistance ◽  
Calcium Signal ◽  
Signal Transducer ◽  
Castration Resistance ◽  
Castration Resistant

Background: prostate cancer (PCa) is a principal cause of cancer-related morbidity and mortality. Castration resistance and metastasis are clinical challenges and continue to impede therapeutic success, despite diagnostic and therapeutic advances. There are reports of the oncogenic activity of genetic suppressor element (GSE)1 in breast and gastric cancers; however, its role in therapy resistance, metastasis, and susceptibility to disease recurrence in PCa patients remains unclear. Objective: this study investigated the role of aberrantly expressed GSE1 in the metastasis, therapy resistance, relapse, and poor prognosis of advanced PCa. Methods: we used a large cohort of multi-omics data and in vitro, ex vivo, and in vivo assays to investigate the potential effect of altered GSE1 expression on advanced/castration-resistant PCa (CRPC) treatment responses, disease progression, and prognosis. Results: using a multi-cohort approach, we showed that GSE1 is upregulated in PCa, while tumor-associated calcium signal transducer 2 (TACSTD2) is downregulated. Moreover, the direct, but inverse, correlation interaction between GSE1 and TACSTD2 drives metastatic disease, castration resistance, and disease progression and modulates the clinical and immune statuses of patients with PCa. Patients with GSE1highTACSTD2low expression are more prone to recurrence and disease-specific death than their GSE1lowTACSTD2high counterparts. Interestingly, we found that the GSE1–TACSTD2 expression profile is associated with the therapy responses and clinical outcomes in patients with PCa, especially those with metastatic/recurrent disease. Furthermore, we demonstrate that the shRNA-mediated targeting of GSE1 (shGSE1) significantly inhibits cell proliferation and attenuates cell migration and tumorsphere formation in metastatic PC3 and DU145 cell lines, with an associated suppression of VIM, SNAI2, and BCL2 and the concomitant upregulation of TACSTD2 and BAX. Moreover, shGSE1 enhances sensitivity to the antiandrogens abiraterone and enzalutamide in vitro and in vivo. Conclusion: these data provide preclinical evidence of the oncogenic role of dysregulated GSE1–TACSTD2 signaling and show that the molecular or pharmacological targeting of GSE1 is a workable therapeutic strategy for inhibiting androgen-driven oncogenic signals, re-sensitizing CRPC to treatment, and repressing the metastatic/recurrent phenotypes of patients with PCa.

scholarly journals The Role of Active Teaching Methods in the Formation of Students’ Cognition and Creativity

2021 ◽  
Vol 7 (6) ◽  
pp. 460-467
Author(s):  
D. Babaev ◽  
M. Bylykova
Keyword(s):  
Teaching Methods ◽  
Educational Institution ◽  
Cognitive Activity ◽  
Great Influence ◽  
Round Table ◽  
Creative Activity ◽  
University Professors ◽  
Active Teaching ◽  
Higher Educational

Higher education as a subject of study of this article in the summer provides for the process of teaching pedagogical disciplines. When conducting seminars in a higher educational institution, the cognitive activity and creativity of students of individual methods of developing physical activity for the purpose of research. Recognition of students and creative activity of children. conversation “brainstorming”, “game activities” “round table” competition of practical works and methods of their discussion is used. It recognizes students on the basis of methods, determining the conditions for the development of culture and creativity. Specific recommendations are given. Student creativity the fact that active learning has a great influence on improvement, also identified. Results in higher educational institution to improve the process of teaching pedagogical disciplines contributes to. This article is recommended for university professors for use in seminars.

scholarly journals The Role of Transformational Leadership and Effective Communication on the Implementation of Patient Safety With Trust as Mediation at X Hospital Jakarta

2021 ◽  
Vol 10 (2) ◽  
pp. 90-102
Author(s):  
Vivi Silvia ◽  
Rokiah Kusumapradja ◽  
Idrus Jus'at
Keyword(s):  
Transformational Leadership ◽  
Patient Safety ◽  
Great Influence ◽  
Effective Communication ◽  
Primary Data ◽  
Global Issue ◽  
Safety Incident ◽  
Professional Nursing Practice ◽  
Patient Safety Incident

Patient safety has become a global issue in the field of health service including hospital. Implementation of patient safety in the hospital requires the involvement of leadership, effective communication and patient’s trust. At X Hospital Jakarta, the root case that frequently occurs on patient safety incident is communication matter. This has an impact on the reoccurrence of patient safety incident. This research aims to analyze the influence of transformational leadership and effective communication towards the implementation of patient safety by trust as mediation. The method of this research is causal associative with quantitative approach. There are 37 nurses as  samples counted with G-Power Statistics application. The technique of collecting data uses primary data questionnaires and is processed by path analysis. The result shows that there is a great influence on transformational leadership towards trust.  Therefore, in implementing patient safety in hospital, it needs program development on transformational leadership; controlling, and evaluating the implementation of effective communication; and improving nursing care as a form of professional nursing practice to create and to maintain patient’s trust towards nurse.

scholarly journals Reporting companies’ performance – in respect of the international financial reporting standards (IFRS)

2013 ◽  
Vol 7 (4-5) ◽  
pp. 107-112 ◽  
Author(s):  
Ildikó Dékán Tamásné Orbán
Keyword(s):  
Financial Reporting ◽  
Evaluation Method ◽  
Great Influence ◽  
International Financial Reporting Standards ◽  
Reporting Standards ◽  
Financial Health ◽  
Financial Reporting Standards ◽  
Globalizing World ◽  
Rule System

The role of information became more important due to rapidly changing technical conditions, market and economic regulations in our globalizing world. Several regulations tend to provide the framework for reporting performance and income of the companies, but in different statements performance is inconsistently presented and many kind of evaluation method exist in the practice. These facts led to the demand of properly assess the financial health of an organization, and created a commonly accepted rule-system, which name was International Financial Reporting Standards (IFRS). In this paper I tend to present the statements, definitions and factors, which can have great influence in representing the performance, income of the company in the frame of the IFRS, and reveal the differences between the other accounting regulations (EU directives, Hungarian Accounting Act) in this field.

scholarly journals ГЕОГРАФІЧНА СТРУКТУРА УРБАНОФЛОР СТЕПОВОЇ ЗОНИ УКРАЇНИ

2016 ◽  
Vol 6 (2) ◽  
pp. 239-245
Author(s):  
G. G. Derevyanska ◽  
O. Z. Glukhov
Keyword(s):  
Great Influence ◽  
Steppe Zone ◽  
Significant Heterogeneity ◽  
Geographic Ranges ◽  
Urban Flora ◽  
Adventive Species ◽  
Number Of Species ◽  
The Impact ◽  
Transition Type

<p>This paper considers the problem of geographic links between the urban flora of steppe zone of Ukraine on the example of the industrial agglomeration Donetsk-Makeyevka, in comparison with urban flora of Kherson and Kirovograd. We presented the detailed description of special features of the composition of geographic elements of the agglomeration flora. It strongly reflects the characteristic features of steppe urban flora, because its territory is affected by the anthropogenic influence at large extend. The spectrum of geographic ranges of species from the agglomeration Donetsk-Makeyevka urban flora numbers 6 types, 12 classes and 130 groups of ranges. The urban flora is both represented by species with wide and local ranges, endemics, that point to its significant heterogeneity. However, compared to the flora of Kherson and Kirovograd, the role of holarctic type of ranges in the agglomeration flora (375 species compared to 400 and 522 species, respectively) is reduced in favor of the polyregional type, reflecting active adventization processes. The holarctic class itself is represented by 150 species (16.7%). It far exceeds the number of species of this class in the regional flora (9.2%), since nearly a half of their number in the observed flora is adventive species. Second place by the number of species in the holarctic class belongs to the European and North American group (13; 1.5%). Apart from this, seven more groups of the class, comprising 1-3 species each, are connected with North America. The polyregional type is represented to a large extent by the species of ruderal habitats. The total number of species of this type is 199. The palaearctic class includes the greatest number of ranges of species from the agglomeration urban flora (31); it consists of 175 species (19.5%), that is less than such index for regional flora (27.3%). The European and Old Mediterranean transition type comprises 97 species (10.8%), that is less than in other urban flora of steppe zone (128; 13.3% in Kherson and 171; 18.0% in Kirovograd). The Nomadic type numbers 132 species (14.7%). One more species – Verbascum marschallianum Ivanina et Tzvelev – is related to the Nomadic and European transition type. The Nomadic and Old Mediterranean transition type includes 93 species (10.4%). This all suggests heterogeneity of urban flora of steppe zone of Ukraine due to their wide geographic connections. On the one hand, they have features of regional flora, which was formed under the great influence of Old Mediterranean flora and has a significant number of narrow-range and endemic species. On the other hand, under the impact of urbanization one can observe the reduction of the role of ranges of the Nomadic type and the enhanced role of the polyregional type due to the significant number of adventive species.</p><p> </p>

scholarly journals A review of fungal influenced corrosion of metals

2021 ◽  
Vol 62 (4) ◽  
pp. 333-339
Author(s):  
Imo Okorie ◽  
Romanus Nwokorie
Keyword(s):  
Growth Pattern ◽  
Metal Surfaces ◽  
Structural Integrity ◽  
Great Influence ◽  
Fungal Growth ◽  
Corrosive Media ◽  
Electrochemical Processes ◽  
Single Mechanism ◽  
Growth Of Fungi

The growth of fungi on the surface of metals has great influence on their structural integrity and failure. Their growth on metal surfaces is determined by their secreted metabolites which enable them to adapt to new environmental and nourishment conditions. Although information on the capacity of fungi to adapt to metal surfaces is scarce, most fungi growing on metal surfaces alter the composition of the metals involving it in the process of functional growth and metabolism. Changes in the composition and colour of the metals are some of the evidences confirming that fungus has penetrated the metal surfaces and use it to satisfy its nutritional need with resultant corrosion. In this work we tried to explain different mechanisms of fungal influenced corrosion from different perspectives ranging from the role of biofilms, corrosive media generation by fungal metabolism processes to electrochemical processes generated by fungal growth on metal surfaces. Finally, no single mechanism can conclusively explain all forms of fungal influenced corrosion because every mechanism is unique and applies to individual fungus, its metabolic biproducts or the growth pattern.

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