Prevalence and associated characteristics of anti-SARS-CoV-2 antibodies in Mexico 5 months after pandemic arrival

Abstract Background Seroprevalence of anti-SARS-CoV-2 antibodies is now available in several world regions to better estimate transmission dynamics. However, to date, there is no epidemiological data regarding anti-SARS-CoV-2 prevalence in Mexico. Therefore, we aimed to determine the prevalence of anti-SARS-CoV-2 antibodies and define the clinical and demographic characteristics associated with seroprevalence. Methods We conducted a cross-sectional serological survey in Ciudad Guadalupe, NL, Mexico. City government employees voluntarily participated during July 2020. Demographic and clinical characteristics were collected at the time of blood sampling to analyze the associated characteristics. IgM/IgG antibodies were determined using a qualitative chemiluminescent immunoassay. Descriptive statistics were used for categorical and continuous variables. Statistical significance was tested using the Chi-squared test, Student’s t-test and the Mann–Whitney. Logistic regression models and the odds ratios (adjusted and unadjusted) were used to estimate the association of demographic and clinical characteristics. Results Of the 3,268 participants included, 193 (5.9%, 95% CI 5.1–6.8) tested positive for IgM/IgG against SARS-CoV-2. Sex, city of residence, and comorbidities did not show any association with having IgM/IgG antibodies. A total of 114 out of 193 (59.1%) subjects with a positive test were asymptomatic, and the odds of being positive were higher in those who reported symptoms of COVID-19 in the previous four weeks to the survey (OR 4.1, 95% CI 2.9–5.5). Conclusions There is a low rate of SARS-CoV-2 infection among government employees that have continuously been working during the pandemic. Six in ten infections were asymptomatic, and seroprevalence is low and still far from herd immunity. Epidemiological surveillance and preventive measures should be mandatory.

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Prevalence and associated characteristics of anti-SARS-CoV-2 antibodies in Mexico 5 months after pandemic arrival

10.21203/rs.3.rs-84890/v1 ◽

2020 ◽

Author(s):

Cristina Díaz-Salazar ◽

Adriana Sánchez-García ◽

René Rodríguez-Gutiérrez ◽

Adrián Camacho-Ortiz ◽

Donato Saldívar-Rodríguez ◽

...

Keyword(s):

Test Performance ◽

Clinical Characteristics ◽

Statistical Significance ◽

Herd Immunity ◽

Epidemiological Data ◽

Epidemiological Surveillance ◽

Continuous Variables ◽

Igg Antibodies ◽

Logistic Regression Models ◽

Student’S T

Abstract Background: Seroprevalence of anti-SARS-CoV-2 antibodies is now available in several world regions to better estimate transmission dynamics. However, to date, there is no epidemiological data regarding anti-SARS-CoV-2 prevalence in Mexico. Therefore, we aimed to determine the prevalence of anti-SARS-CoV-2 antibodies and define the clinical and demographic characteristics associated with seroprevalence in a institutionalized population.Methods: We conducted a serological survey in Ciudad Guadalupe, NL, Mexico. Institutionalized employees voluntarily participated during July 2020. Demographic and clinical characteristics were collected at the time of blood sampling to analyze the associated characteristics. IgM/IgG antibodies were determined using a qualitative chemiluminescent immunoassay. We reported the raw and the adjusted seroprevalence for test performance characteristics. Descriptive statistics were used for categorical and continuous variables. Statistical significance was tested using the Chi-squared test, Student’s t-test and the Mann-Whitney. Logistic regression models and the odds ratios (adjusted and unadjusted) were used to estimate the association of demographic and clinical characteristics.Results: Of the 3,268 participants included, 193 (5.9%, 95% CI 5.1-6.8) tested positive for IgM/IgG against SARS-CoV-2. Adjusted prevalence by the immunoassay diagnostic performance resulted in a prevalence of 5.7 (95% CI 4.9-6.6). Gender, city of residence, and comorbidities did not show any association with having IgM/IgG antibodies. A total of 114 out of 193 (59.1%) subjects with a positive test were asymptomatic, and the odds of being positive were higher in those who reported symptoms of COVID-19 in the previous four weeks to the survey (OR 4.1, 95% CI 2.9-5.5).Conclusions: There is a low rate of SARS-CoV-2 infection among institutionalized employees that have continuously been working during the pandemic. Six in ten infections were asymptomatic, and seroprevalence is low and still far from herd immunity. Epidemiological surveillance and preventive measures should be mandatory.

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PREVALENCE OF PARACOCCIDIOIDOMYCOSIS INFECTION BY INTRADERMAL REACTION IN RURAL AREAS IN ALFENAS, MINAS GERAIS, BRAZIL

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652014000400002 ◽

2014 ◽

Vol 56 (4) ◽

pp. 281-285 ◽

Cited By ~ 9

Author(s):

Evandro Monteiro de Sá Magalhães ◽

Carla de Fátima Ribeiro ◽

Carla Silva Dâmaso ◽

Luiz Felipe Leomil Coelho ◽

Roberta Ribeiro Silva ◽

...

Keyword(s):

Rural Areas ◽

Alcohol Intake ◽

Paracoccidioides Brasiliensis ◽

Statistical Significance ◽

Epidemiological Data ◽

Population Based ◽

Minas Gerais ◽

Delayed Type Hypersensitivity ◽

Local Health ◽

Cross Sectional

This study aimed to estimate the prevalence of paracoccidioidal infection by intradermal reaction (Delayed-Type Hypersensitivity, DTH) to Paracoccidioides brasiliensis in rural areas in Alfenas, Southern Minas Gerais (MG) State, Brazil, and to assess risk factors (gender, occupation, age, alcohol intake and smoking) associated with infection. We conducted a population-based cross-sectional study using intradermal tests with gp 43 paracoccidioidin in 542 participants, who were previously contacted by local health agents and so spontaneously attended the test. Participants underwent an interview by filling out a registration form with epidemiological data and were tested with an intradermal administration of 0.1 mL of paracoccidioidin in the left forearm. The test was read 48 hours after injection and was considered positive if induration was greater than or equal to 5 mm. Out of 542 participants, 46.67% were positive to the skin test. Prevalence increased in accordance with an increase of age. There was statistical significance only for males. Occupation, alcohol intake and smoking habits were not significantly associated with the risk of paracoccidioidomycosis infection. There is relevance of paracoccidioidomycosis infection in such rural areas, which suggests that further epidemiological and clinical studies on this mycosis should be done in the southern part of Minas Gerais State.

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Prevalence and Associated Factors of Hypertension Among Adults living in the City of Bandundu, Republic Democratic Republic of the Congo. A Population-Based Cross-Sectional Study.

10.21203/rs.3.rs-144209/v1 ◽

2021 ◽

Author(s):

Ferdinand Mukoso ◽

Aliocha Nkodila ◽

François Lepira ◽

Pascal Bayauli ◽

Yves Lubenga ◽

...

Keyword(s):

Risk Factors ◽

Cardiovascular Risk ◽

Sleep Duration ◽

Subclinical Atherosclerosis ◽

Statistical Significance ◽

Epidemiological Data ◽

Cluster Sampling ◽

Cross Sectional Survey ◽

Cross Sectional ◽

Factors Associated

Abstract Background and aim: In limited resource settings, reliable epidemiological data generated from hypertension high risk geographical areas or people is a prerequisite for the planning of proven and effective interventions. The aim of the present survey was to assess the prevalence, awareness, control and factors associated with hypertension in adults living in the port City of Bandundu, located in the southwestern part of DRC.Methods: a cross-sectional survey using a modified WHO STEPwise questionnaire for data collection during face-to face interviews was conducted from 1st May to 30th October 2018. A multistage cluster sampling method was used and inclusion criteria were age ≥18 years and informed consent. Information on demographic parameters, behavioral lifestyles, anthropometric and blood pressure (BP) measurements was obtained. Hypertension was defined as an average of two BP ≥ 140/90 mmHg or self-reported history of antihypertensive medication use. Independent factors associated with hypertension were identified using logistic regression analysis. P<0.05 defined the level of statistical significance. Results: The prevalence of hypertension was 34,5% (Women 36,6 %). Older age (p<0.001), subclinical atherosclerosis (p= 0.021), sleep duration <8 hours (p<0.002), diabetes mellitus (p<0.001), and obesity (p= 0.009) emerged as main cardiovascular risk factors associated with hypertension.Conclusion: Hypertension was characterized by a high prevalence, low rate of awareness and suboptimal BP control, high cardiovascular risk and associated sleep duration and obesity as modifiable risk factors.

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Multiple sclerosis in First Nations Canadians: A pilot comparison study

Multiple Sclerosis Journal - Experimental Translational and Clinical ◽

10.1177/2055217316666093 ◽

2016 ◽

Vol 2 ◽

pp. 205521731666609

Author(s):

Ruth Ann Marrie ◽

Nicholas Hall ◽

A Dessa Sadovnick

Keyword(s):

Multiple Sclerosis ◽

First Nations ◽

Clinical Characteristics ◽

Statistical Significance ◽

Diagnostic Delay ◽

Psychiatric Disease ◽

Cross Sectional ◽

Genetic Characteristics ◽

Standardized Interview ◽

Observational Design

Background Genetic and clinical characteristics associated with multiple sclerosis (MS) may differ by ethnicity but few studies have evaluated whether characteristics of MS differ between individuals according to First Nations (FN) ethnicity. Objective Using a cross-sectional observational design, we compared clinical and genetic characteristics between people with MS of FN and non-FN ethnicity. Methods We recruited participants of FN ethnicity with MS. We conducted a medical records review for each participant followed by a standardized interview and drawing of blood samples. The blood underwent genetic analyses for several HLA alleles. We compared the study sample with 127 non-FN MS participants from another study conducted in the same region using the same data collection procedures. Results We included 144 participants with MS, of whom 17 (11.8%) self-identified as FN. The age of symptom onset was earlier and the diagnostic delay shorter among FN participants although these differences did not reach statistical significance. As compared to non-FN participants, FN participants with MS had increased odds of comorbid psychiatric disease (OR 5.38; 95% CI: 1.84–15.8), and were less likely to be HLA-DRB1*1501 positive (OR 0.32; 95% CI: 0.11–0.96). Conclusion Genetic and clinical characteristics of MS differ among Canadians of FN and non-FN ethnicity.

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1491. Active Norovirus Surveillance in Children Under 5 Years with Diarrhea after Rotavirus Vaccine Introduction in Argentina (2017–2019)

Open Forum Infectious Diseases ◽

10.1093/ofid/ofz360.1355 ◽

2019 ◽

Vol 6 (Supplement_2) ◽

pp. S543-S543

Author(s):

Angela Gentile ◽

Juan Stupka ◽

Juan I Degiuseppe ◽

María del Valle Juárez ◽

Maria Florencia Lucion ◽

...

Keyword(s):

Acute Diarrhea ◽

Epidemiological Data ◽

Cross Sectional Study ◽

Epidemiological Surveillance ◽

Watery Diarrhea ◽

Rotavirus Vaccine ◽

Cross Sectional ◽

Vaccine Introduction ◽

Rotavirus Vaccination ◽

Children Under 5

Abstract Background Acute diarrhea is one of the leading causes of infant morbidity and mortality. Argentina introduced massive rotavirus vaccination in 2015. In several countries, this introduction has changed the distribution of enteropathogens. The decrease in the prevalence of rotavirus has been described at the expense of an increase in Norovirus (NoV) activity worldwide. The aim of this study was to analyze the role of NoV in acute diarrhea cases in outpatient children under 5 years of age and their epidemiological profile. Methods A prospective and cross-sectional study in <5 years outpatients attended for acute diarrhea in Children’s Hospital “Dr. Ricardo Gutiérrez” in Buenos Aires, Argentina, between July 2017 and March 2019 was conducted. Active epidemiological surveillance was performed with a specific case reporting form. Stool samples were tested for NoV (RT-qPCR). Clinical and epidemiological data were recorded. Results A total of 252 patients were enrolled and 235 stools samples were tested. Median of age was 22.3 months (IQR: 11–30), 58.7% were male. The most frequent symptoms were fever and vomiting in 63.1% and 53%, respectively; 52% had watery diarrhea, 45.2% had moderate diarrhea according to Vesikari Scale, 95.6% were normohydrated and 22% had a household member with diarrhea. There were no immunocompromised children. A 72% had received rotavirus vaccine, 86% of them with full scheme. From samples tested, 27% (n = 63) were NoV positive. NoV was found throughout the year and the frequency of detection was higher in January and June (summer and winter in Argentina). Regarding genetic diversity the most frequent genogroup was GII (65%; 41/63) and genotype GII.P16-GII.4 Sydney (48%; 20/41). Bacterial co-infection was observed in 35%. Compared with negative cases, NoV were younger (18 vs. 20 months; P < 0.001) and were associated with higher prevalence of rotavirus vaccination (88% vs. 66%; P = 0.001). No statistically difference was found regarding to gender, clinical outcome and severity. Conclusion NoV was detected at high frequency (27%) in children presenting moderate acute diarrhea, mainly in those who received rotavirus vaccine. Regarding sporadic acute diarrhea cases in children, it is important to consider NoV as a frequent etiological agent. Disclosures All authors: No reported disclosures.

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Herd immunity to SARS-COV-2 in the population of the Southern regions of the Far East of Russia

Acta biomedica scientifica ◽

10.29413/abs.2021-6.5.25 ◽

2021 ◽

Vol 6 (5) ◽

pp. 253-273

Author(s):

A. Yu. Popova ◽

V. S. Smirnov ◽

E. B. Ezhlova ◽

A. A. Melnikova ◽

O. E. Trotsenko ◽

...

Keyword(s):

Statistical Significance ◽

Herd Immunity ◽

Far East ◽

Amur Region ◽

Reproductive Number ◽

Pasteur Institute ◽

Scientific Center ◽

Cross Sectional ◽

Population Immunity ◽

The Far East

The aim: to study the structure and dynamics of population immunity to SARSCoV-2 of the population of the Southern Regions of the Far East (SRFE): Khabarovsk, Primorsky Krai and Amur Region during the COVID-19 epidemic in 2020.Materials and methods. The work was carried out according to the program for assessing population immunity to SARS -CoV-2 of the population of the Russian Federation according to the methodology developed by the Rospotrebnadzor with the participation of the St. Petersburg Pasteur Institute. The study was approved by the ethical committee of the St. Petersburg Pasteur Institute. The selection of participants was carried out by a questionnaire method using cloud technologies. The volunteers were randomized by age by stratification into 7 age groups: 1–17, 18–29, 30–39, 40–49, 50–59, 60–69, 70+ years old. Territorial randomization consisted in limiting the engaging of volunteers – no more than 30 people from one enterprise. After the initial cross-sectional study, a 3-stage seromonitoring was carried out, in which the same volunteers participated. Antibodies to the SARS-CoV-2 nucleocapsid were determined in peripheral blood serum by the enzyme immunoassay using an appropriate set of reagents produced by the State Scientific Center for Medical and Biological Sciences of the Rospotrebnadzor (Obolensk). Statistical analysis was performed using the Excel package. The confidence interval for the proportion was calculated using the A. Wald, J. Wolfowitz method with A. Agresti, B.A. Coull’s correction. The statistical significance of the differences was calculated online using a specialized calculator. The statistical significance of the differences was assessed with a probability of p˂ 0.05, unless otherwise indicated.Results. In a comparative analysis, the highest morbidity was observed in the Khabarovsk Territory, the lowest – in the Primorsky Territory. The level of seroprevalence among the population of the region was 19.6 % (95 % CI: 18.2–21.1) in the Khabarovsk Territory, 19.6 % (95 % CI: 18.1–21.2) in the Primorsky Territory19,6 % and 45,5 % (95 % CI: 43.7–47.3) in the Amur region. The highest seroprevalence was noted among 1–17 years old children, mainly due to the subgroup of 14–17-years-olds. The smallest proportion of seropositive was found among 40–49-year-olds in the Khabarovsk Territory (14.7 %, 95 % CI: 11.2–18.6), 18–28-yearolds in the Primorsky Territory (13.3 %, 95 % CI: 10.0–17.1) and 30–39-year-olds in the Amur Region (36.3 %, 95% CI: 31.7–41.6). No statistically significant dependence of seroprevalence on territorial and occupational factors has been established, with the exception of an increase in the proportion of seropositive medical workers in Primorsky Territory. In the process of 3-stage seromonitoring, a regular increase in the proportion of seropositive people was revealed in all SRFE. The resulting tendency is correctly described by a second-order polynomial. A relationship was revealed between the number of convalescents and persons in contact with them, which made it possible to calculate the base reproductive number (R0) in the range from 1.4 (Primorsky Territory) to 2.4 (Amur Region). Analysis of seroprevalent volunteers showed that the number of asymptomatic individuals varied from 94.1 % (95 % CI: 92.8–95.3) to 98.3 % (95 % CI: 98.8–99.2). This indicates that most of the volunteers had COVID-19 asymptomatically.Conclusions. A comparative study showed the prevalence of seroprevalence in the Amur Region compared with the Khabarovsk and Primorsky Territories. The relationship between the number of convalescents and persons in contact with them was noted. The value of the base R0 is calculated. It has been shown that more than 90 % of seropositive individuals in the COVID-10 SRFE were asymptomatic.

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High Community SARS-CoV-2 Antibody Seroprevalence in a Ski Resort Community, Blaine County, Idaho, US. Preliminary Results

10.1101/2020.07.19.20157198 ◽

2020 ◽

Cited By ~ 4

Author(s):

Colleen McLaughlin ◽

Margaret K. Doll ◽

Kathryn T Morrison ◽

William L McLaughlin ◽

Terry OConnor ◽

...

Keyword(s):

Disease Surveillance ◽

Population Distribution ◽

A Priori ◽

Herd Immunity ◽

Sensitivity Analyses ◽

Igg Antibodies ◽

Cross Sectional ◽

Diagnostic Uncertainty ◽

Ski Resort ◽

Zip Code

Community-level seroprevalence surveys are needed to determine the proportion of the population with previous SARS-CoV-2 infection, a necessary component of COVID-19 disease surveillance. In May, 2020, we conducted a cross-sectional seroprevalence study of IgG antibodies for nucleocapsid of SARS-CoV-2 among the residents of Blaine County, Idaho, a ski resort community with high COVID-19 attack rates in late March and Early April (2.9% for ages 18 and older). Participants were selected from volunteers who registered via a secure web link, using prestratification weighting to the population distribution by age and gender within each ZIP Code. Participants completed a survey reporting their demographics and symptoms; 88% of volunteers who were invited to participate completed data collection survey and had 10 ml of blood drawn. Serology was completed via the Abbott Architect SARS-CoV-2 IgG immunoassay. Primary analyses estimated seroprevalence and 95% credible intervals (CI) using a hierarchical Bayesian framework to account for diagnostic uncertainty. Stratified models were run by age, sex, ZIP Code, ethnicity, employment status, and a priori participant-reported COVID-19 status. Sensitivity analyses to estimate seroprevalence included base models with post-stratification for ethnicity, age, and sex, with or without adjustment for multi-participant households. IgG antibodies to the virus that causes COVID-19 were found among 22.7% (95% CI: 20.1%, 25.5%) of residents of Blaine County. Higher levels of antibodies were found among residents of the City of Ketchum 34.8% (95% CI 29.3%, 40.5%), compared to Hailey 16.8% (95%CI 13.7%, 20.3%) and Sun Valley 19.4% (95% 11.8%, 28.4%). People who self-identified as not believing they had COVID-19 had the lowest prevalence 4.8% (95% CI 2.3%, 8.2%). The range of seroprevalence after correction for potential selection bias was 21.9% to 24.2%. This study suggests more than 80% of SARS-CoV-2 infections were not reported. Although Blaine County had high levels of SARS-CoV-2 infection, the community is not yet near the herd immunity threshold.

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Thrombocytosis in Febrile Illness in Children of Age Three Months to 14 Years at a Tertiary Care Hospital of Nepal

Janaki Medical College Journal of Medical Science ◽

10.3126/jmcjms.v6i1.20568 ◽

2018 ◽

Vol 6 (1) ◽

pp. 5-14

Author(s):

Sajan Kumar Thakur ◽

Pramod Kattel

Keyword(s):

Bacterial Infection ◽

Statistical Significance ◽

Univariate Analysis ◽

Tertiary Care ◽

Febrile Illness ◽

Care Hospital ◽

Continuous Variables ◽

Chi Square ◽

Cross Sectional ◽

Chi Square Test

Background and Objectives: Acute febrile illness is common presentation in children. Thrombocytosis can be used as a predictor of febrile illness in children. The objectives of this study were to find out the prevalence and causes of thrombocytosis among febrile children and to access the utility of platelet count as a potential predictor of serious bacterial infection alone or in association with other predictors.Material and Methods: A cross-sectional descriptive study was done in 290 febrile children following convenience sampling method. Statistical analysis was done using SPSS. Univariate analysis using chi-square test for categorical and independent samples and “t” test for continuous variables were done.Results: Thrombocytosis was present in 13.1% of cases. Most cases had mild thrombocytosis (10%) whereas moderate thrombocytosis was present in 2.8% of cases and 0.3% cases had severe thrombocytosis. Lymphocyte level, erythrocyte sedimentation rate and C-reactive protein also showed a significant association with thrombocytosis (p<0.05). Serious bacterial infection was present in 209 cases out of which 30 children had thrombocytosis but was not significant compared to children with non serious bacterial infection with thrombocytosis (p=0.171).Conclusion: Thrombocytosis was found to have an association with febrile illnesses. More so, it indicated a fair discriminate ability to diagnose infectious cause of disease. However there was no statistical significance between thrombocytosis and serious bacterial infection.

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Impact of the Second Epidemic Wave of SARS-CoV-2: Increased Exposure of Young People

Frontiers in Public Health ◽

10.3389/fpubh.2021.715192 ◽

2021 ◽

Vol 9 ◽

Author(s):

Lisandru Capai ◽

Shirley Masse ◽

Toscane Fourié ◽

Dorine Decarreaux ◽

Jean Canarelli ◽

...

Keyword(s):

Neutralizing Antibodies ◽

Herd Immunity ◽

Wave Period ◽

Quantitative Detection ◽

Igg Antibodies ◽

Cross Sectional ◽

Clinical Laboratories ◽

Serological Surveillance ◽

Second Wave ◽

Neutralization Antibody Titer

We aimed to use serological surveillance based on serial cross-sectional sampling of residual sera obtained from clinical laboratories to compare the differences in age and sex profiles of infected persons in the first and second waves of SARS-CoV-2 in Corsica, France. Residual sera were obtained, including samples from individuals of all ages collected for routine screening or clinical management by clinical laboratories. All the sera collected were tested for the presence of anti-SARS-CoV-2 IgG using a kit for semi-quantitative detection of IgG antibodies against the S1 domain of the viral spike protein (ELISA-S). Samples that were borderline and positive in ELISA-S were tested with an in-house virus neutralization test. During the second-wave period, we collected between 6 November, 2020 and 12 February, 2021, 4,505 sera from patients aged 0–101 years (60.4% women). The overall weighted seroprevalence of residual sera collected during the second-wave period [8.04% (7.87–9.61)] was significantly higher than the overall weighted seroprevalence estimated at the end of the first wave between 16 April and 15 June, 2020 [5.46% (4.37–7.00)] (p-value = 0.00025). Ninety-eight (30.1%) of the 326 samples tested in the VNT assay had a positive neutralization antibody titer. Estimated seroprevalence increased significantly for men [odds ratio (OR) OR = 1.80 (1.30–2.54); p-value = 0.00026] and for people under 30 years of age [OR = 2.17 (1.46–3.28); p-value = 0.000032]. This increase was observed in young adults aged 20–29 years among whom antibody frequencies were around four-fold higher than those observed at the end of the first wave. In conclusion, our seroprevalence estimates, including the proportion of the participants who had produced neutralizing antibodies, indicate that in February, 2021 the population of Corsica was still far from being protected against SARS-Cov-2 by “herd immunity.”

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Hepatitis E Virus among Animal Handlers and Non-Animal Handlers in Osun State, Nigeria

Asian Journal of Advanced Research and Reports ◽

10.9734/ajarr/2020/v13i430316 ◽

2020 ◽

pp. 35-48

Author(s):

I. R. Gidado ◽

F. A. Osundare ◽

I. O. Okonko ◽

O. O. Opaleye

Keyword(s):

Hepatitis E Virus ◽

Statistical Significance ◽

Hepatitis E ◽

Cross Sectional Study ◽

Effective Control ◽

Igg Antibodies ◽

Cross Sectional ◽

Low Prevalence ◽

Biology Laboratory ◽

Animal Handlers

Aim: Increase in the epidemiological information is important for effective control of hepatitis E virus (HEV). This study was conducted to determine the prevalence of HEV among butchers, pig handlers and non-animal handlers in Osun State, Nigeria. Study Design: Cross-sectional study. Place and Duration of Study: Molecular Biology Laboratory, College of Health Sciences, Ladoke Akintola University of Technology, Isale Osun, Osogbo, Nigeria, between June 2015 and July 2019. Methods: A total of 180 blood samples were obtained and screened for HEV from cohorts of 90 animal handlers (69 butchers and 21 pig handlers) and 90 non-animal handlers. Questionnaires on HEV were administered to obtain a demographic characteristic of the participants. Anti-hepatitis E viruses were also screened using HEV ELISA kit. Results: Results showed an overall prevalence of HEV to be 21.7%. The rate of anti-HEV IgG/IgM antibodies was higher among butchers (27.5%), followed by non-animal handlers (18.9%) and was least among the pig handlers (14.3%) while the two IgM positive persons were butchers and non-animal handlers. However, 39(21.7%) of the 180 samples were positive for either anti-HEV IgG antibodies (37/180, 20.6%) or anti-HEV IgM (2/180, 1.1%). Also, the rate of anti-HEV IgG antibodies was higher among butchers (26.1%), followed by non-animal handlers (17.8%) and the pig handlers (14.3%) had the least. The two IgM positive persons were butchers (1.4%), non-animal handlers (1.1%) and pig handlers had a zero prevalence. There was no statistical significance in the prevalence of HEV IgG and HEV IgM in animal handlers as compared to non-animal handlers (P > 0.05). One (1.4%) of the butchers and 1 (1.1%) of non-animal handlers showed evidence of recent HEV infection by being positive to HEV IgM. Sources of drinking water were the only HEV predisposition factor for HEV (P =0.023). Conclusion: This study reported an acute HEV infection in a butcher and a non-animal handler in Osun State, Nigeria. No prevalence rates of acute HEV infection was observed among pig handlers in Osun State, Nigeria. The study also showed a low prevalence of anti-HEV IgG antibodies among these study populations. Proper hygiene is recommended for further reduction in HEV transmission in Nigeria. Aim: Increase in the epidemiological information is important for effective control of hepatitis E virus (HEV). This study was conducted to determine the prevalence of HEV among butchers, pig handlers and non-animal handlers in Osun State, Nigeria. Study Design: Cross-sectional study. Place and Duration of Study: Molecular Biology Laboratory, College of Health Sciences, Ladoke Akintola University of Technology, Isale Osun, Osogbo, Nigeria, between June 2015 and July 2019. Methods: A total of 180 blood samples were obtained and screened for HEV from cohorts of 90 animal handlers (69 butchers and 21 pig handlers) and 90 non-animal handlers. Questionnaires on HEV were administered to obtain a demographic characteristic of the participants. Anti-hepatitis E viruses were also screened using HEV ELISA kit. Results: Results showed an overall prevalence of HEV to be 21.7%. The rate of anti-HEV IgG/IgM antibodies was higher among butchers (27.5%), followed by non-animal handlers (18.9%) and was least among the pig handlers (14.3%) while the two IgM positive persons were butchers and non-animal handlers. However, 39(21.7%) of the 180 samples were positive for either anti-HEV IgG antibodies (37/180, 20.6%) or anti-HEV IgM (2/180, 1.1%). Also, the rate of anti-HEV IgG antibodies was higher among butchers (26.1%), followed by non-animal handlers (17.8%) and the pig handlers (14.3%) had the least. The two IgM positive persons were butchers (1.4%), non-animal handlers (1.1%) and pig handlers had a zero prevalence. There was no statistical significance in the prevalence of HEV IgG and HEV IgM in animal handlers as compared to non-animal handlers (P > 0.05). One (1.4%) of the butchers and 1 (1.1%) of non-animal handlers showed evidence of recent HEV infection by being positive to HEV IgM. Sources of drinking water were the only HEV predisposition factor for HEV (P =0.023). Conclusion: This study reported an acute HEV infection in a butcher and a non-animal handler in Osun State, Nigeria. No prevalence rates of acute HEV infection was observed among pig handlers in Osun State, Nigeria. The study also showed a low prevalence of anti-HEV IgG antibodies among these study populations. Proper hygiene is recommended for further reduction in HEV transmission in Nigeria.

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