scholarly journals Comparison of the accumulation of macro- and microelements in the bone marrow and bone of wild and farmed red deer (Cervus elaphus)

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Katarzyna Tajchman ◽  
Aleksandra Ukalska-Jaruga ◽  
Marek Bogdaszewski ◽  
Monika Pecio ◽  
Pawel Janiszewski
Keyword(s):  
Bone Marrow ◽  
Body Weight ◽  
Cervus Elaphus ◽  
Inductively Coupled Plasma ◽  
The Body ◽  
Entire Body ◽  
Wild Animals ◽  
The Mean ◽  
Wild Deer ◽  
Mean Concentration

Abstract Background The cells of the entire body, including the skeletal system, especially of young animals, may derive from the bone marrow in which they multiply. Therefore, it is important to assess whether the diet and quality of life of deer have a significant impact on the elemental composition of bone and bone marrow, which can directly affect their health and growth. The aim of this study was to determine the concentrations of macro- (Ca, calcium, P, phosphorus, Mg, magnesium, K, potassium, Na, sodium) and microelements (Li, lithium, Cr, chromium, Mn, manganese, Co, cobalt, Cu, copper, Zn, zinc, Se, selenium, Mo, molybdenum, and Sn, tin) accumulated in the bone marrow and bones of deer (Cervus elaphus). The study was carried out on 15 young stags divided into two groups: farmed and wild animals. The concentrations of macro- and microelements were analysed using the inductively coupled plasma mass spectrometry technique. This research expands our knowledge on this topic, which so far has not been extensively studied. Results The mean content of K, Na, Zn and Se in the bone marrow of farmed animals was significantly higher than in wild deer, whereas the mean content of Ca, P, Mg, K, Na and Li in the bones was higher in wild animals than in farmed individuals (p < 0.05). In addition, the mean concentration of Cr, Mn, Cu, Se and Mo in the bones of the analysed animals differed significantly (p < 0.05) and was higher in the farmed deer. The mean concentration of Se in the bone marrow of wild deer decreased with the increase of the body weight (p < 0.05). In turn, the mean content of Mn in the bone marrow and of Mo in the bones of the animals was significantly positively correlated with the animals’ body weight (p < 0.05). Conclusions The obtained results indicated different levels of micro- and macro-components in the body of farmed and wild deer, though without clear and strong variations. Generally, the higher level of macronutrients in the bones of wild deer may be related to the higher physiological importance of these minerals for life activities in the natural environment and to the limited supply of balanced food. On the other hand, the higher levels of microelements in the tissues of farmed animals may result from their significantly better nutritional status in the first year of life, achieved through appropriate nutrition as well as diet supplementation of adult females.

scholarly journals Accumulation of Toxic Elements in Bone and Bone Marrow of Deer Living in Various Ecosystems. A Case Study of Farmed and Wild-Living Deer

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2151
Author(s):  
Katarzyna Tajchman ◽  
Aleksandra Ukalska-Jaruga ◽  
Marek Bogdaszewski ◽  
Monika Pecio ◽  
Katarzyna Dziki-Michalska
Keyword(s):  
Bone Marrow ◽  
Cervus Elaphus ◽  
Inductively Coupled Plasma ◽  
Toxic Elements ◽  
Red Deer ◽  
Cadmium Concentration ◽  
Inductively Coupled ◽  
Significant Difference ◽  
In The Wild ◽  
The Mean

The aim of the study was to determine the concentrations of toxic elements accumulated in the bone marrow and bones (Cervus elaphus). The studies were carried out on two groups of young stags: farmed (n = 6) and wild (n = 9). Their body weights were measured and bone and bone marrow samples were collected. The concentrations of toxic elements were analyzed using the inductively coupled plasma mass spectrometry technique. The mean aluminum content in the bone marrow and bones of the farmed animals was significantly higher than in the wild group (p < 0.05). The mean concentration of arsenic, barium and lead in the bones of the wild red deer was significantly higher than in the bones of the farmed animals (p < 0.05), while the cadmium concentration in the bones of the farmed red deer exceeded the value determined in the wild animals. A significant difference was found between the mean concentrations of aluminum, arsenic, barium, lead, vanadium and nickel in the bone marrow and bones of both red deer groups (p < 0.05). Although the study involved animals living in an uncontaminated area, the concentrations of some heavy metals were higher than values reported from industrial regions.

scholarly journals Determination of dexamethasone in saliva

1996 ◽  
Vol 42 (8) ◽  
pp. 1238-1242 ◽  
Author(s):  
J H Thijssen ◽  
C C Gispen-de Wied ◽  
G M van Heeswijk ◽  
W Veeman
Keyword(s):  
Body Weight ◽  
Oral Administration ◽  
Large Range ◽  
Plasma Concentrations ◽  
The Body ◽  
The Mean ◽  
Mean Concentration

Abstract We conducted experiments to demonstrate that the determination of dexamethasone in saliva is a valuable tool to assess dexamethasone concentrations in the body after administration of this synthetic corticosteroid. After extraction of saliva, a RIA involving highly specific commercially available antibodies was established and evaluated. This RIA was easy to conduct, reliable, and sensitive enough to measure dexamethasone until approximately 24 h after oral administration of 1 mg per 70 kg of body weight. On the morning after the administration, the mean concentration in saliva was 540 pmol/L. Over a large range (10(3)-10(6) pmol/L), plasma concentrations correlated very well with salivary concentrations; plasma contained six times more dexamethasone than saliva.

Effect of High Plane of Nutrition on Blood Biochemical Profile and Onset of Puberty in Prepubertal HF X Kankrej Crossbred Heifers

2019 ◽  
Vol 15 (02) ◽  
pp. 14-17
Author(s):  
K K Hadiya ◽  
A J Dhami ◽  
D V Chaudhari ◽  
P M Lunagariya
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Plasma Levels ◽  
Age Of Onset ◽  
The Body ◽  
Monthly Interval ◽  
Control Group ◽  
Blood Biochemical ◽  
Lower Protein ◽  
The Mean

This study was initiated on 24 prepubertal Holstein x Kankrej crossbred heifers of nearly identical age (7-9 months) and body weight (130-140 kg) at University farm to evaluate the effect of high plane of nutrition on blood biochemical and minerals profile and the age at puberty. Twelve heifers were managed under routine farm feeding (control) and the rest 12 under ideal optimum feeding regime (treatment) that included extra 1 kg concentrate, 30 g min mix and ad-lib dry fodder. The body weight and ovarian ultrasonography together with blood sampling was carried out at monthly interval from 10 to 18 months of age to study the ovarian dynamics and blood biochemical changes. High plane of nutrition to growing heifers was beneficial in reducing the age of onset of puberty (by 2-3 months) compared to routine farm fed group. The mean plasma total protein and cholesterol concentrations showed a rising trend with significant variations from 10 to 16 months of age, where it got mostly stabilized indicating adult profile. The activity of enzymes GOT and GPT also rose gradually and significantly from 10 months till 14-15 months of age, and thereafter it remained more or less static till 18 months of age. The levels of both these enzymes were higher, with lower protein and cholesterol, in control than the treatment group from 15-16 months of age onwards. The mean plasma levels of both calcium and phosphorus increased gradually and significantly with advancing age till 16-17 months of age, with little higher values in supplemented than a control group. The plasma levels of zinc, iron, copper, and cobalt also showed rising trend with significant differences between 10th and 12th-14th months of age, and from 15th to 18th months of age the levels were statistically the same in all the groups with slightly higher values in the treatment group.

Respiratory Exchange and Body Size in the Aldabra Giant Tortoise

1971 ◽  
Vol 55 (3) ◽  
pp. 651-665 ◽  
Author(s):  
G. M. HUGHES ◽  
R. GAYMER ◽  
MARGARET MOORE ◽  
A. J. WOAKES
Keyword(s):  
Body Weight ◽  
Body Size ◽  
Metabolic Rate ◽  
Relative Proportion ◽  
The Body ◽  
O2 Consumption ◽  
Weight Range ◽  
Testudo Hermanni ◽  
The Mean ◽  
Good Agreement

1. The O2 consumption and CO2 release of nine giant tortoises Testudo gigantea (weight range 118 g-35·5 kg) were measured at a temperature of about 25·5°C. Four European tortoises Testudo hermanni (weight range 640 g-2·16 kg) were also used. The mean RQ values obtained were 1·01 for T. gigantea and 0·97 for T. hermanni. These values were not influenced by activity or size. 2. The data was analysed by plotting log/log regression lines relating body weight to O2 consumption. Both maximum and minimum metabolic rates recorded for each individual T. gigantea showed a negative correlation with body weight. For active rates the relation was O2 consumption = 140·8W0·97, whereas for inactive animals O2 consumption = 45·47W0·82. 3. The maximum rates were obtained from animals that were observed to be active in the respirometer and the minimum rates from animals that remained quiet throughout. The scope for activity increased with body size, being 82 ml/kg/h for animals of 100 g and 103 ml/kg/h for 100 kg animals. The corresponding ratio between maximum and minimum rates increases from about 2 to 6 for the same weight range. 4. Values for metabolic rate in T. hermanni seem to be rather lower than in T. gigantea. Analysis of the relative proportion of the shell and other organs indicates that the shell forms about 31% of the body weight in adult T. hermanni but only about 18% in T. gigantea of similar size. The shell is not appreciably heavier in adult T. gigantea (about 20%). 5. Data obtained for inactive animals is in good agreement with results of other workers using lizards and snakes. Previous evidence suggesting that chelonians show no reduction in metabolic rate with increasing size is not considered to conflict with data obtained in the present work.

scholarly journals Structural Organization of the Optic Lobe of Grey Breasted Helmeted Guinea Fowl (*Numida meleagris galeata*) at Pre-Hatch Study

2016 ◽  
Vol 7 (2) ◽  
pp. 26
Author(s):  
Wanmi Nathaniel ◽  
Onyeanusi I. Barth ◽  
Nzalak J. Oliver ◽  
Aluwong Tanang
Keyword(s):  
Body Weight ◽  
Structural Organization ◽  
Optic Lobe ◽  
Guinea Fowl ◽  
The Body ◽  
Whole Body ◽  
Steady Increase ◽  
Numida Meleagris ◽  
The Mean ◽  
Immature Cells

<p class="jbls-body"><span lang="EN-GB">A total of one hundred and seventy-three fertilized eggs were used for morphometry, gross and histological studies. At day 4 of incubation, the mean body weight of the helmeted guinea fowl embryo was 0.6401 ± 0.0211 g. It was at day 10 of incubation that there was an increase in the whole body weight of the embryo to be 0.8650 ± 0.676 g. The whole brain weight indicated relative increased at day 4 as compared to that of the whole body weight. Graphically, there were steady increase in the body, brain and optic lobe weights. Histologically, cells and neurones that make up the optic lobe is probably as a result of the migration of immature cells from the ventricular neuroepithelium. </span></p>

scholarly journals The effect of artificial sweeteners on body weight of mice

2021 ◽  
Vol 93 (2) ◽  
Author(s):  
Iyad Ali ◽  
Naser Shraim ◽  
Wafaa’ Atrash ◽  
Aisha Sirafi ◽  
Huda Abadi
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Fluid Intake ◽  
Weight Maintenance ◽  
Zingiber Officinale ◽  
The Body ◽  
Thymus Vulgaris ◽  
Artificial Sweeteners ◽  
The Mean ◽  
Daily Intakes

Artificial Sweeteners (AS) are synthetic sugar substitutes that have sweetening potency hundreds of times more than the table sugar (sucrose). Artificial sweeteners are regarded as attractive alternatives to sugar as they add no calories to food intake. There are many hypotheses suggesting that AS may enhance appetite and cause weight gain. The aim of this study was to evaluate the effect of AS on food intake, fluid intake and body weight of mice. Acceptable daily intakes of AS solutions were administered orally to different set of mice for four weeks. The body weight, food consumption and fluid intake were measured. At the same time, the effect of Zingiber officinale extracts (natural appetite suppressor), Thymus vulgaris extracts (natural appetite inducer) and cyproheptadine (an appetite stimulant drug) on body weight of mice was evaluated. Artificial sweeteners consumption cause insignificant changes in body weight (p>0.05). However, the mean consumption of food and solutions varies significantly for some groups. The consumption of AS has no significant effect on body weight and may contribute to weight maintenance and energy balance as substitutes to high caloric sugar

scholarly journals Co-Therapy of Pegylated G-CSF and Ghrelin for Enhancing Survival After Exposure to Lethal Radiation

2021 ◽  
Vol 12 ◽  
Author(s):  
Juliann G. Kiang ◽  
Min Zhai ◽  
Bin Lin ◽  
Joan T. Smith ◽  
Marsha N. Anderson ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Body Weight ◽  
Water Consumption ◽  
Bone Marrow Cells ◽  
The Body ◽  
Photon Radiation ◽  
Vehicle Group ◽  
Spleen Weight ◽  
Histopathological Analysis ◽  
Marrow Cells

Exposure to ionizing radiation (radiation injury, RI) in nuclear-related episode is evident to be life-threatening. RI occurs at levels of organs, tissues, cytosols, or nucleus. Their mechanisms are still not fully understood. FDA approves pegylated granulocyte colony-stimulating factor (Neulasta™, Peg-G-CSF) for acute hematopoietic syndrome and has been shown to save lives after lethal RI. We aimed to test whether Ghrelin enhanced Peg-G-CSF’s efficacy to save more lives after lethal RI. B6D2F1/J female mice were used for the study. They received 9.5 Gy (LD50/30 at 0.4 Gy/min) emitted from the 60Co-γ-photon radiation facility. Peg-G-CSF was injected subcutaneously at 1 mg/kg once on days 1, 8, and 15 after irradiation. Ghrelin contains 28 amino acid and is a hunger peptide that has been shown to stimulate food intake, promote intestinal epithelial cell proliferation, elevates immunity, inhibits brain hemorrhage, and increases stress-coping. Ghrelin was injected subcutaneously at 113 μg/kg once on days 1, 2, and 3 after irradiation. Survival, body weight, water consumption, hematology, spleen weight, splenocytes, bone marrow cells, and histology of bone marrow and ileum were performed. We observed that radiation resulted in 30-days survival by 30%. RI decreased their body weights and water consumption volumes. On the 30th day post-RI, platelets and WBCs such as basophils, eosinophils, monocytes, lymphocytes, neutrophils and leukocytes were still significantly decreased in surviving mice. Likewise, their RBC, hemoglobin, hematocrit, and splenocytes remained low; splenomegaly was found in these mice. Bone marrow in surviving RI animals maintained low cellularity with high counts of fat cells and low counts of megakaryocytes. Meanwhile, ileum histology displayed injury. However, mice co-treated with both drugs 24 h after RI resulted in 30-days survival by 45% above the vehicle group. Additionally, the body-weight loss was mitigated, the acute radiation syndrome was reduced. This co-therapy significantly increased neutrophils, eosinophils, leukocytes, and platelets in circulation, inhibited splenomegaly, and increased bone marrow cells. Histopathological analysis showed significant improvement on bone marrow cellularity and ileum morphology. In conclusion, the results provide a proof of concept and suggest that the co-therapy of Peg-G-CSF and Ghrelin is efficacious to ameliorate RI.

Abstract 1129: Obesity-related Elevations in Soluble P-selectin are Derived From Endothelium and Dependent on Expression of Leukocyte P-selectin Glycoprotein Ligand-1

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Kevin J Wickenheiser ◽  
Peter F Bodary ◽  
Kristina Bahrou ◽  
Daniel T Eitzman
Keyword(s):  
Bone Marrow ◽  
Body Weight ◽  
Leptin Receptor ◽  
The Body ◽  
Wild Type ◽  
Post Transplant ◽  
Time Period ◽  
Increased Risk ◽  
Soluble P ◽  
Deficient Mice

Background : Obesity is associated with proinflammatory changes and an increased risk for vascular disease complications. The tissue source and mechanism by which soluble P-selectin (sPsel) is generated in obesity are unclear. Methods and Results : Soluble p-selectin (sPsel) levels were measured in the circulation from lean wild type and obese leptin receptor deficient mice (LepR−/−) at 4 and 10 weeks of age. In wild-type mice body weight increases from 13+/−2 to 20+/−3 grams over this time period while the body weight increases from 15+/−2 to 38+/−5 grams in LepR−/− mice. At 4 weeks of age sPsel levels were 103+/−8mg/mL in wild-type mice vs. 138+/−9 ng/mL in LepR−/− mice, p=0.048. By 10 wks of age sPsel increased to 112 +/− 2 in wild-type mice and 182 +/− 9 in LepR−/− mice, p=0.00005. In order to determine if the obesity-induced rise in sPsel is regulated by leukocyte Psgl-1, bone marrow transplantation was performed from Psgl+/+ or Psgl−/− donors into irradiated LepR−/−recipients. At 4 weeks post-transplant, sPsel levels were 166 +/−6 ng/mL in LepR−/− mice receiving Psgl+/+ marrow and 45 +/− 4 ng/mL in LepR−/− mice receiving Psgl−/− marrow, p=0.0000004. In order to determine if the sPsel in LepR−/− mice originated from the endothelium versus platelets, we transplanted Psel−/− bone marrow into irradiated LepR−/−mice. At 4 weeks post transplant, sPsel levels were 153 +/−3 ng/mL in LepR−/− mice receiving Psel−/− bone marrow and were not significantly different from LepR−/− mice receiving Psel+/+ bone marrow (166 +/−6 ng/mL, p=0.06). By 10 weeks post transplant, mice gained even more weight and levels were 377+/−51 ng/mL in LepR−/− mice receiving Psel+/+ bone marrow and 370+/−73 ng/mL in LepR−/− mice receiving Psel−/− bone marrow, p=0.87. Conclusions : These data suggest that the increase in sPsel observed in obesity is primarily derived from the endothelium and that this process is regulated by leukocyte Psgl-1.

Unsteady disturbances of streaming motions around bodies

1989 ◽  
Vol 209 ◽  
pp. 385-403 ◽  
Author(s):  
H. M. Atassi ◽  
J. Grzedzinski
Keyword(s):  
Wave Equation ◽  
Body Surface ◽  
Source Term ◽  
The Body ◽  
Entire Body ◽  
Two Dimensional ◽  
Mean Flow ◽  
Inhomogeneous Wave ◽  
Inhomogeneous Wave Equation ◽  
The Mean

For small-amplitude vortical and entropic unsteady disturbances of potential flows, Goldstein proposed a partial splitting of the velocity field into a vortical part u(I) that is a known function of the imposed upstream disturbance and a potential part ∇ϕ satisfying a linear inhomogeneous wave equation with a dipole-type source term. The present paper deals with flows around bodies with a stagnation point. It is shown that for such flows u(I) becomes singular along the entire body surface and its wake and as a result ∇ϕ will also be singular along the entire body surface. The paper proposes a modified splitting of the velocity field into a vortical part u(R) that has zero streamwise and normal components along the body surface, an entropy-dependent part and a regular part ∇ϕ* that satisfies a linear inhomogeneous wave equation with a modified source term.For periodic disturbances, explicit expressions for u(R) are given for three-dimensional flows past a single obstacle and for two-dimensional mean flows past a linear cascade. For weakly sheared flows, it is shown that if the mean flow has only a finite number of isolated stagnation points, u(R) will be finite along the body surface. On the other hand, if the mean flow has a stagnation line along the body surface such as in two-dimensional flows then the component of u(R) in this direction will have a logarithmic singularity.For incompressible flows, the boundary-value problem for ϕ* is formulated in terms of an integral equation of the Fredholm type. The theory is applied to a typical bluff body. Detailed calculations are carried out to show the velocity and pressure fields in response to incident harmonic disturbances.

scholarly journals Geochemical assessment of heavy metal pollution of river basin in Nigeria using stream sediment

2020 ◽  
Vol 7 (2) ◽  
pp. 198
Author(s):  
Oluwafunso Oladipo Awosusi ◽  
Adeshina Luqman Adisa
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Enrichment Factor ◽  
River Basin ◽  
Metal Pollution ◽  
Heavy Metal Pollution ◽  
Stream Sediment ◽  
The Body ◽  
The Mean ◽  
Mean Concentration

Heavy metal pollution has been a source of health problems in humans. These metals are persistent, toxic, non-degradable and often take a long time to be eliminated from the body. This study is, therefore, designed to assess heavy metal pollution of River Basin in Nigeria. Seventy stream sediment samples were systematically collected from an area, approximately 400km2, latitude 7O 00’ and 7O 15’N and longitude 5O 11’ and 5O 19’E. The pollution status of the sediments by heavy metals were assessed by Enrichment Factor (EF), Pollution Load Index (PLI) and Geo-accumulation Index (Igeo). The concentrations of the heavy metals were also compared with United States Environmental Protection Agency (USEPA) Sediment quality guidelines (SQG). The samples were dried in the laboratory, disaggregated, sieved to minus 80 (<177 microns) mesh size using nylon sieve. The sieved samples were, then, digested and the concentrations of As, Co, Fe, Mn, Ni, Pb, V and Zn were determined by Wavelength Dispersive X-ray Fluorescence Spectrometry (WD-XRFS). Results revealed that the mean concentrations of the heavy metals are in the order V>Zn>Pb>As>Ni>Co>Fe>Mn. Furthermore, the mean concentration of lead exceeded both the average world shale and the USEPA SQG values. However, the mean concentration of cobalt, nickel, manganese and zinc were lower than the average world shale values for these elements. The Enrichment Factor (EF) revealed that cobalt was moderately enriched while arsenic and lead were significantly enriched in the sediments. On the basis of the geoaccumulation index, the stream sediments were largely uncontaminated except at some sites that were moderately to strongly contaminated by As and Pb.  

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