scholarly journals Phyto-nano-hybrids of Ag-CuO particles for antibacterial activity against drug-resistant pathogens

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Syed Baker ◽  
Perianova Olga ◽  
Rukosueva Tatiana ◽  
Potkina Nadezhda ◽  
Garkusha Tatyana ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Preliminary Investigation ◽  
Inhibition Zone ◽  
Xrd Analysis ◽  
Multidrug Resistant ◽  
Drug Resistant ◽  
Hybrid Particles ◽  
Tem Analysis ◽  
Visible Spectra ◽  
Shoulder Peak

Abstract Background The present study reports the antibacterial potential of phyto-nano-hybrid particles Ag-CuO (silver-copper oxide) against drug-resistant pathogens isolated from a Russian hospital in Krasnoyarsk, Siberia. The synthesis of nano-hybrid was achieved by phytogenic source by using leaves of Murraya koenigii. The nano-hybrid particles were well characterized using hyphenated techniques and results of the antibacterial assay was tabulated. Results The UV-visible spectra displayed absorption at 420 nm with the shoulder peak at 355 nm indicating the hybridization. The FTIR analysis revealed the presence of phenol, amine, methyl, carbohydrate and aromatic as major functional groups. The XRD analysis revealed the presence of Bragg’s intensities at 2 theta angle depicting the crystalline nature of Ag-CuO nano-hybrid. The TEM analysis displayed the polydispered properties of Ag-CuO nano-hybrid with the size in the range of 60–80 nm exhibiting different shapes ranging from spherical, rod and oval. The antibacterial activity of Ag-CuO nano-hybrid was tested against multidrug-resistant pathogens that resulted in highest activity against P. aeruginosa strain with an inhibition zone of 14 mm in diameter. The MIC concentrations ranged from 0.3125 to 2.5 μg/ml and broth dilution assay displayed dose-dependent properties of Ag-CuO nano-hybrid particles. Conclusion The obtained results are interesting to report the preliminary insight to develop biocompatible hybrid particles to combat drug-resistant pathogens. The developed nano-hybrid particles displayed activity against all the test pathogens investigated against both Gram-positive and Gram-negative bacteria. Thus, the study forms preliminary investigation to report nano-hybrid particles as broad spectrum antibacterial agents.

scholarly journals Antibacterial activity of graphene oxide nanosheet against multidrug resistant superbugs isolated from infected patients

2020 ◽  
Vol 7 (7) ◽  
pp. 200640 ◽  
Author(s):  
Md. Toasin Hossain Aunkor ◽  
Topu Raihan ◽  
Shamsul H. Prodhan ◽  
H. S. C. Metselaar ◽  
Syeda Umme Fahmida Malik ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Graphene Oxide ◽  
Antimicrobial Property ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Drug Resistant ◽  
Graphene Nanosheet ◽  
Solid Agar ◽  
Good Biocompatibility ◽  
Agar Well Diffusion Method

Graphene oxide (GO) is a derivative of graphene nanosheet which is the most promising material of the decade in biomedical research. In particular, it has been known as an antimicrobial nanomaterial with good biocompatibility. In this study, we have synthesized and characterize GO and checked its antimicrobial property against different Gram-negative and Gram-positive multidrug drug resistant (MDR) hospital superbugs grown in solid agar-based nutrient plates with and without human serum through the utilization of agar well diffusion method, live/dead fluorescent staining and genotoxicity analysis. No significant changes in antibacterial activity were found in these two different conditions. We also compare the bactericidal capability of GO with some commonly administered antibiotics and in all cases the degree of inhibition is found to be higher. The data presented here are novel and show that GO is an effective bactericidal agent against different superbugs and can be used as a future antibacterial agent.

scholarly journals Evaluation of Antibacterial Activity of Essential Oils and Their Combination against Multidrug-Resistant Bacteria Isolated from Skin Ulcer

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Eshetu Gadisa ◽  
Hydar Usman
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Antibacterial Effect ◽  
Wound Care ◽  
In Vitro Study ◽  
Inhibition Zone ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria

Background. Emerging of multidrug-resistant bacteria can compromise the effectiveness of antibiotics used to treat skin infections. Those bacteria imposed public health problems and questioning medical care in the 21st century. In this circumstance, essential oils of medicinal plants origin are supreme sources of structural and functionally divergent compounds, which inhibited the growth of common wound colonizing MRSA and ESBL producing P. aeruginosa. The aim of this study was to evaluate the combined antibacterial activity of essential oils extracted from Rumex abyssinicus, Cucumis pustulatus, and Discopodium penninervium against multidrug-resistant (MDR) isolates of skin ulcers. Methods. Essential oils (EOs) were extracted from aerial parts of R. abyssinicus, C. pustulatus, and D. penninervium with steam distillation. A mixture of each oil (1 : 1) was adsorbed to a disc and placed on Mueller Hinton Agar. Then, minimum zone of inhibition and bactericidal concentration of EOs was measured after incubeted for 18–24 hours at 37 °C. Their combined antibacterial effect was determined by the fractional inhibitory concentration index. Results. The antibacterial activity of mixed oil varied in their doses and bacteria species, of which a mixture of essential oil of R. abyssinicus and D. penninervium had inhibition zone (32 mm); its MIC and MBC values range from 1-2 μl/ml against MRSA. It had an inhibition zone (36 mm), MIC value 4 μl/ml, and MBC (8 μl/ml) against ESBL producing P. aeruginosa, whereas combined effects of R. abyssinicus and C. pustulatus had MIC values ranging from 2–8 μl/ml for E. coli and K. pneumoniae and 2 μl/ml for MRSA. There was a strong synergistic effect between R. abyssinicus and D. penninervium and promising antibacterial effect more specifically on MRSA and P. aeruginosa. Conclusion. This in vitro study of the combined effect of EOs has significant antibacterial activity on wound colonizing bacteria and reduces delaying wound healing as that of modern drugs tested in parallel. Hence, further structural elucidation of active compounds helps us to properly design or synthesis of topical antibiotics for wound care.

scholarly journals Synergistic antibacterial effects of colistin in combination with aminoglycoside, carbapenems, cephalosporins, fluoroquinolones, tetracyclines, fosfomycin, and piperacillin on multidrug resistant Klebsiella pneumoniae isolates

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0244673
Author(s):  
Julalak C. Ontong ◽  
Nwabor F. Ozioma ◽  
Supayang P. Voravuthikunchai ◽  
Sarunyou Chusri
Keyword(s):  
Antibacterial Activity ◽  
Klebsiella Pneumoniae ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Antibacterial Effects ◽  
Drug Resistant Bacteria ◽  
Multidrug Resistant Pathogens ◽  
Conventional Antibiotics ◽  
Global Health Problem

Multidrug resistant Enterobacterales have become a serious global health problem, with extended hospital stay and increased mortality. Antibiotic monotherapy has been reported ineffective against most drug resistant bacteria including Klebsiella pneumoniae, thus encouraging the use of multidrug therapies as an alternative antibacterial strategy. The present works assessed the antibacterial activity of colistin against K. pneumoniae isolates. Resistant isolates were tested against 16 conventional antibiotics alone and in combination with colistin. The results revealed that all colistin resistant isolates demonstrated multidrug resistance against the tested antibiotics except amikacin. At sub-inhibitory concentrations, combinations of colistin with amikacin, or fosfomycin showed synergism against 72.72% (8 of 11 isolates). Colistin with either of gentamicin, meropenem, cefoperazone, cefotaxime, ceftazidime, moxifloxacin, minocycline, or piperacillin exhibited synergism against 81.82% (9 of 11 isolates). Combinations of colistin with either of tobramycin or ciprofloxacin showed synergism against 45.45% (5 in 11 isolates), while combinations of colistin with imipenem or ceftolozane and tazobactam displayed 36.36% (4 of 11 isolates) and 63.64% (7 of 11 isolates) synergism. In addition, combinations of colistin with levofloxacin was synergistic against 90.91% (10 of 11 isolates). The results revealed that combinations of colistin with other antibiotics could effectively inhibit colistin resistant isolates of K. pneumoniae, and thus could be further explore for the treatment of multidrug resistant pathogens.

scholarly journals Green synthesis of silver nanoparticle using rambutan (Nephelium lappaceum L.) peel extract and its antibacterial activity against Salmonella parathypi A.

2018 ◽  
Vol 154 ◽  
pp. 01024 ◽  
Author(s):  
Puji Lestari ◽  
Indah Pratiwi ◽  
Any Juliani
Keyword(s):  
Antibacterial Activity ◽  
Tap Water ◽  
Inhibition Zone ◽  
Green Method ◽  
X Ray ◽  
Color Changes ◽  
Nephelium Lappaceum ◽  
Visible Spectra ◽  
Uv Visible ◽  
Peel Extract

Ag nanoparticles (AgNPs) have been synthesized via green method using rambutan (Nephelium lappaceum L.) peel (RP) extract. RP extract was prepared by washing the RP using tap water thoroughly and boiling it in distilled water at 70°C for 60 min. RP extract and AgNO3 were used as the starting materials for the synthesis of AgNPs. RP extract was added to 10-3 M AgNO3 solution with a ratio by volume of 1:10 (RP : AgNO3), stirred at room temperature. The solution’s color changes from reddish to dark brown, indicating the reduction of Ag+ in the solution. The synthesized AgNPs were characterized using UV-Visible Spectrophotometer, FTIR Spectrophotometer, and Scanning Electron Microscopy-Energy-dispersive X-ray Spectroscopy (SEM-EDS) instruments. UV-Visible spectra show that the AgNPs have the maximum absorption band at 450 nm which is typical for AgNPs. The FTIR spectra revealed that the protein in RP extract acts as the capping agent for the synthesized AgNPs. The synthesized AgNPs were tested for their antibacterial activity against Salmonella parathypi A. The antibacterial test shows that 50 μL of AgNPs resulted in the inhibition zone of 4 mm against the aforementioned microorganism.

scholarly journals Gram-scale synthesis of splat-shaped Ag–TiO2 nanocomposites for enhanced antimicrobial properties

2020 ◽  
Vol 11 ◽  
pp. 1119-1125
Author(s):  
Mohammad Jaber ◽  
Asim Mushtaq ◽  
Kebiao Zhang ◽  
Jindan Wu ◽  
Dandan Luo ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Multidrug Resistant ◽  
Bacterial Strains ◽  
Gram Negative ◽  
E Coli ◽  
Contagious Diseases ◽  
Good Biocompatibility

The control over contagious diseases caused by pathogenic organisms has become a serious health issue. The extensive usage of antibiotics has led to the development of multidrug-resistant bacterial strains. In this regard, metal-oxide-based antibacterial nanomaterials have received potential research interest due to the efficient prevention of microorganism growth. In this study, splat-shaped Ag–TiO2 nanocomposites (NCs) were synthesized on the gram scale and the enhanced antibacterial properties of TiO2 in the presence of silver were examined. The formation of Ag–TiO2 NCs was analyzed through various characterization techniques. The cell viability experimental results demonstrated that the Ag–TiO2 NCs have good biocompatibility. The antibacterial activity of the prepared Ag–TiO2 NCs was tested against the Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) bacterial strains. The Ag–TiO2 NCs exhibited promising and superior antibacterial properties compared to TiO2 nanospheres as confirmed by the bacterial growth and inhibition zone. The improvement in the antibacterial activity was attributed to the synergistic effect of the hybrid nature of TiO2 nanoparticles in the presence of Ag.

scholarly journals Coloration and Multi-Functionalization of Polypropylene Fabrics with Selenium Nanoparticles

Polymers ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 2483
Author(s):  
Tarek AbouElmaaty ◽  
Shereen A. Abdeldayem ◽  
Shaimaa M. Ramadan ◽  
Khaled Sayed-Ahmed ◽  
Maria Rosaria Plutino
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Xrd Analysis ◽  
Industrial Applications ◽  
Uv Protection ◽  
Selenium Nanoparticles ◽  
Hydrothermal Conditions ◽  
Color Strength ◽  
Protection Factor ◽  
Tem Analysis

In this study, we developed a new approach for depositing selenium nanoparticles (SeNPs) into polypropylene (PP) fabrics via a one-step process under hydrothermal conditions by using an IR-dyeing machine to incorporate several functionalities, mainly coloration, antibacterial activity and ultraviolet (UV) protection. The formation, size distribution, and dispersion of the SeNPs were determined using X-ray diffraction (XRD), ultraviolet-visible (UV/Vis), transmission electron microscopy (TEM) and the color strength, fastness, antibacterial properties, and UV protection of the treated fabrics were also explored. The UV-Vis spectra and TEM analysis confirmed the synthesis of spherical well-dispersed SeNPs and the XRD analysis showed the successful deposition of SeNPs into PP fabrics. The obtained results demonstrate that the SeNPs-PP fabrics is accompanied by a noticeable enhancement in measurements of color strength, fastness, and UV-protection factor (UPF), as well as excellent antibacterial activity. Viability studies showed that SeNPs-PP fabrics are non-toxic against wi-38cell line. In addition, the treated SeNPs-PP fabrics showed an increase in conductivity. The obtained multifunctional fabrics are promising for many industrial applications such as the new generation of curtains, medical fabrics, and even automotive interior parts.

scholarly journals Antibacterial activity test on ethanol extract fraction of Kirinyuh (Chromolaena odorata L.) leaves for multi-drug resistant organisms bacteria

2021 ◽  
Vol 9 (1) ◽  
pp. 26
Author(s):  
Endang Sulistyarini Gultom ◽  
Tri Hartanti ◽  
Hasnaul Maritsa ◽  
Eko Prasetya
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Bacterial Resistance ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Drug Resistant ◽  
Chromolaena Odorata ◽  
Extended Spectrum Beta Lactamase ◽  
Extract Fraction

The resistance of pathogenic bacteria to antibiotics is increasing due to antibiotics with incorrect doses, wrong diagnostics, and the wrong target. Bacteria that have been resistant to several antibiotics are called multi-drug resistant organisms (MDRO) bacteria. Bacterial resistance to some antibiotics requires alternative herbal treatments, one of which is the Chromolaena odorata L. Research must therefore be conducted on the antibacterial activity of the ethanol extract fraction of C. odorata L. leaves for MDRO bacteria, such as Staphylococcus lugdunensis methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa extended-spectrum beta-lactamase (ESBL), and Klebsiella pneumoniae ESBL. This study aims to determine the antibacterial activity of the ethanol extract of Kirinyuh leaves (C. odorata L.) and the antibacterial activity of the fractionation against MDRO bacteria. Test results of kirinyuh leaf ethanol extract for S. lugdunensis MRSA, P. aeruginosa ESBL, and K. pneumoniae ESBL bacteria each resulted in an inhibition zone with an average diameter of 11.6 mm (strong), 11.5 mm (strong), and 11.13 mm (strong), respectively. Testing the antibacterial activity of the ethanol fraction against MDRO bacteria can show antibacterial activity against all tested bacteria, namely Fraction 5. The results of the antibacterial activity of fraction 5 against K. pneumoniae ESBL, P. aeruginosa ESBL, and S. lugdunensis MRSA bacteria with the formation of inhibition zones formed of 10.2 mm (strong), 8.8 mm (moderate), and 7.9 mm (moderate), respectively. The results of thin-layer chromatography showed that the secondary metabolites contained in the fifth fraction were terpenoids, steroids, and flavonoids.

scholarly journals Syzygium cumini Mediated Green Synthesis of Silver Nanoparticles for Reduction of 4-Nitrophenol and Assessment of its Antibacterial Activity

2021 ◽  
Author(s):  
Samadhan R. Waghmode ◽  
Amol A. Dudhane ◽  
Vaibhav P. Mhaindarkar
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Average Particle Size ◽  
Xrd Analysis ◽  
Reduction Reaction ◽  
Device Fabrication ◽  
Syzygium Cumini ◽  
Tem Analysis ◽  
Elemental Silver ◽  
Biosynthesized Agnps

The biosynthesis of silver nanoparticles (AgNPs) has become more significant in the recent years owing to its applications in catalysis, imaging, drug delivery, nano-device fabrication and in medicine. We propose the synthesis of silver nanoparticles from the plant extract of Syzygium cumini and evaluation of its antibacterial and chemocatalytic potential. Synthesis of AgNPs carried out by using aqueous silver nitrate. The UV–Vis absorption spectrum of the synthesized AgNPs showed a broad absorption peak at 470 nm. TEM analysis shows the morphology of AgNPs as a hexagonal matrix with average particle size is about 50 nm. XRD analysis displays the crystalline structure of AgNPs. The presence of elemental silver was confirmed with EDX analysis. FTIR analysis shows that amide groups present in proteins are dominant reducing agents and play an important role in the bioreduction of Ag+ ions to Ag0. The bioreduced AgNPs demonstrated significant catalytic properties in a reduction reaction of 4-nitrophenol to 4-aminophenol using NaBH4 in an aqueous condition. The biosynthesized AgNPs have potent antibacterial activity against common clinical pathogens. Considering the remarkable antibacterial activity against common pathogenic microorganisms, AgNPs can be used in the pharmaceutical industries.

scholarly journals Antibacterial Activity of Medicinal Flowers against Multi Drug Resistant E. coli

2021 ◽  
Vol 41 (01) ◽  
pp. 166-168
Author(s):  
Iqra Nazir
Keyword(s):  
Antibacterial Activity ◽  
Taraxacum Officinale ◽  
Multidrug Resistant ◽  
Achillea Millefolium ◽  
Rosa Damascena ◽  
Drug Resistant ◽  
E Coli ◽  
And Control ◽  
Woodfordia Fruticosa ◽  
Hyssopus Officinalis

The antibiotic resistance is creating a serious challenge to treat diseases especially against multidrug resistant (MDR) E. coli. The present study was conducted to evaluate the antibacterial activity of seven different flowers ethanolic extracts named as Achillea millefolium, Bombax ceiba, Chrysanthemum cinerarifolium, Hyssopus officinalis, Rosa damascena Miller, Taraxacum officinale Weber and Woodfordia fruticosa against MDR E. coli through spot test and minimum inhibitory concentrations (MIC) test. The results showed all the seven extracts have significant antibacterial activity against MDR E. coli and control. The MIC of Rosa damascena Miller, Bombax ceiba was 3.125 mg/ml and Taraxacum officinale Weber was 12.5 mg/ml for MDR E. coli. Similarly, Achillea millefolium, Hyssopus officinalis, Chrysanthemum cinerarifolium MIC value was 25 mg/ml and 12.5 mg/ml for Woodfordia fruticosa Kurtz. The current study reveals that these flowers possess strong antibacterial activity and thus can be used as potential antimicrobial agent against various resistant bacterial pathogens

Sign in / Sign up

Export Citation Format

Share Document