scholarly journals A New Outbreak in Sea Bass Farming in Turkey: Aeromonas veronii

2021 ◽  
Vol 72 (3) ◽  
pp. 3051
Author(s):  
T TANSEL TANRIKUL ◽  
E DINÇTÜRK
Keyword(s):  
Clinical Symptoms ◽  
Aegean Sea ◽  
Sea Bass ◽  
Economic Loss ◽  
The Body ◽  
Production Cycle ◽  
Preventive Strategy ◽  
Bacterial Diseases ◽  
Aeromonas Veronii ◽  
Diseased Fish

Turkey produces most of the European sea bassin Europe and bacterial diseases are the main cause of economic loss during the production cycle. This research presents the first extended study of the Aeromonas veronii infection in sea bass on the Turkish coast of the Aegean Sea. An outbreak was observed in three different sea bass farms and diseased fish with clinical symptoms were sampled. Abdominal distention, hemorrhages on the body and anus, enlarged spleen and liver, and hemorrhages in the internal organs were detected from clinical and pathological examinations. Biochemical and molecular identification confirmed the pathogen to be A.veronii.The histopathological observations demonstrated that the pathogen caused bacterial colonies in the fibrous connective tissue, granuloma, and vacuolar degeneration.The primary causes of the disease were proved through an infection experiment. 80% and 90% mortality were calculated in 0.85 x 108 CFU ml-1 and 1.28 x 108 CFU ml-1 experimentally infected groups with clinical and pathological signs of the disease respectively. Recently, the pathological symptoms of the disease had been confused with pasteurellosis infection in cage farms but the presence of A.veronii has been confirmed in the current study. A detailed study is needed to investigate the overall status of the disease in the Aegean Sea in order to design an appropriate preventive strategy.

scholarly journals A First Report of Aeromonas veronii Infection of the Sea Bass, Lateolabrax maculatus in China

2021 ◽  
Vol 7 ◽  
Author(s):  
Baotun Wang ◽  
Can Mao ◽  
Juan Feng ◽  
Yong Li ◽  
Jianmei Hu ◽  
...  
Keyword(s):  
Sea Bass ◽  
The Body ◽  
Sensitivity Testing ◽  
Internal Organs ◽  
Aeromonas Veronii ◽  
Tissue Degeneration ◽  
Lateolabrax Maculatus ◽  
Diseased Fish ◽  
Lethal Dosage ◽  
Physiological And Biochemical

The sea bass, Lateolabrax maculatus is commercially farmed in Zhuhai, located in the Guangdong Province of China. L. maculatus in aquaculture have suffered acute death, characterized by ulcerations on the body surface, congestion, and hemorrhage in internal organs such as liver, kidney, and spleen. The dominant infecting strain of bacteria isolated from the kidneys of diseased fish was identified as Aeromonas veronii (strain 18BJ181). This identification was based on analysis of morphological, physiological, and biochemical features, as well as 16S rRNA and gyrB gene sequences. Drug sensitivity testing showed that the strain 18BJ181 isolate was resistant to four antibacterial drugs, including amoxicillin, madinomycin, penicillin and sulfamethoxazole, while moderately sensitive to erythromycin and rifampicin. The detection of growth characteristics showed that the strain 18BJ181 exhibited adaptability to the environment. In addition, some virulence genes, such as aer, act, gcaT, tapA and fla, were detected in the strain 18BJ181. The median lethal dosage of the strain 18BJ181 isolate in L. maculatus was 8.5 × 105 and 4.2 × 105 cfu/g under the conditions of intraperitoneal injection and intramuscular injection, respectively. The experimentally induced infection showed that the 18BJ181 isolate caused considerable histological lesions in L. maculatus, including tissue degeneration, necrosis, and different degrees of hemorrhage. These results provided evidence for a more comprehensive understanding of A. veronii strain 18BJ181 infection in L. maculatus.

scholarly journals Bacterial flora associated with diseased freshwater ornamental fish

2017 ◽  
Vol 61 (4) ◽  
pp. 445-449 ◽  
Author(s):  
Natalia Walczak ◽  
Krzysztof Puk ◽  
Leszek Guz
Keyword(s):  
Bacterial Species ◽  
Bacterial Flora ◽  
Ornamental Fish ◽  
Citrobacter Freundii ◽  
Bacterial Diseases ◽  
Aeromonas Veronii ◽  
Causative Agents ◽  
Diseased Fish ◽  
Shewanella Baltica ◽  
Tof Ms

AbstractIntroduction:Ornamental fish can suffer from different bacterial diseases. Among them the most prevalent are infections caused byAeromonas, Shewanella, Citrobacter, Plesiomonas, Edwardsiella, andPseudomonas.But there is a broad spectrum of rarely identified bacteria which may be causative agents of diseases. The aim of the study was to determine the species of bacteria pathogenic for fish which are prevalent in aquariums.Material and Methods:Bacteria were isolated from infected ornamental fish from pet shops and private aquariums in the Lublin region in 2015 and classified to species using MALDI-TOF MS.Results:A total of 182 isolates from ornamental fish were identified. The most frequent bacteria found in diseased fish wereAeromonas veronii(30.8% of total number of strains),A. hydrophila(18.7%),Shewanella putrefaciens(7.1%),Citrobacter freundii(7.1%),Pseudomonas spp. (7.1%),Shewanella baltica(4.9%), andPlesiomonas shigelloides(3.3%).Conclusion:Isolated bacterial species are facultative pathogens for fish and humans and may be isolated from fish without apparent symptoms of the disease.

scholarly journals Isolation, characterization and sequence determination of Noda virus from sea bass (Dicentrarchus labraxL.) reared in freshwater and marine facilities in Greece

2017 ◽  
Vol 56 (2) ◽  
pp. 105
Author(s):  
E. XYLOURI (Ε. ΞΥΛΟΥΡΗ) ◽  
J. KOTZAMANIS (Ι. ΚΟΤΖΑΜΑΝΗΣ) ◽  
F. ATHANASSOPOULOU (ΑΘΑΝΑΣΟΠΟΥΛΟΥ Φ.) ◽  
L. DONG ◽  
A. ARGYROKASTRITIS (ΑΛ. ΑΡΓΥΡΟΚΑΣΤΡΙΤΗΣ) ◽  
...  
Keyword(s):  
Clinical Symptoms ◽  
Dicentrarchus Labrax ◽  
Sea Bass ◽  
Rt Pcr ◽  
Sequence Determination ◽  
Tissue Samples ◽  
Diseased Fish ◽  
Polymerase Chain ◽  
Virus Isolates

The present study aims το characterize Noda virus isolates from freshwater and marine fish, specifically from species Dicentrarchus labrax (sea bass). Previous works reported the detection of Noda virus only by using PCR, in cultured Dicentrarchus labrax showing clinical symptoms. Here we describe the isolation of Noda virus from symptomatic sea bass cultured in sea and freshwater facilities in Greece. The virus was isolated in the continuous cell line SSN -1, exhibiting characteristic cytopathic effect, vacuolation and degeneration of the monolayer. In parallel, in all cerebral tissue samples isolated from infected individuals, a 255 bp viral fragment has been detected, using Reverse Transcription - Polymerase Chain Reaction (RT-PCR) and nested PCR. Comparison of the amplified sequence was detected in diseased fish in European farms and in other piscine species revealed a high nucleotide homology.

scholarly journals Respiratory tract infections in children with allergic asthma on allergen immunotherapy during influenza season

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yuyun Li ◽  
Dongming Wang ◽  
Lili Zhi ◽  
Yunmei Zhu ◽  
Lan Qiao ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Allergic Asthma ◽  
Respiratory Tract Infections ◽  
Allergen Immunotherapy ◽  
Clinical Symptoms ◽  
Influenza Season ◽  
The Body ◽  
History Of ◽  
Tract Infections ◽  
Stage 1

AbstractTo describle how respiratory tract infections (RTIs) that occurred in children with allergic asthma (AA) on allergen immunotherapy (AIT) during an influenza season. Data including clinical symptoms and treatment history of children (those with AA on AIT and their siblings under 14 years old), who suffered from RTIs during an influenza season (Dec 1st, 2019–Dec 31st, 2019), were collected (by face to face interview and medical records) and analyzed. Children on AIT were divided into 2 groups: stage 1 (dose increasing stage) and stage 2 (dose maintenance stage). Their siblings were enrolled as control. During the study period, 49 children with AA on AIT (33 patients in stage 1 and 16 patients in stage 2) as well as 49 children without AA ( their siblings ) were included. There were no significant differences in occurrences of RTIs among the three groups (p > 0.05). Compared with children in the other two groups, patients with RTIs in stage 2 had less duration of coughing and needed less medicine. Children on AIT with maintenance doses had fewer symptoms and recovered quickly when they were attacked by RTIs, which suggested that AIT with dose maintenance may enhance disease resistance of the body.

scholarly journals Knowledge and Practices of Back Care, Experience in Colombian Children

2021 ◽  
Vol 8 ◽  
pp. 2333794X2110234
Author(s):  
Sonia Carolina Mantilla Toloza ◽  
Carlos Alberto Jaimes Guerrero ◽  
Piedad Rocio Lerma Castaño
Keyword(s):  
Education Program ◽  
School Context ◽  
Intervention Group ◽  
First Grade ◽  
The Body ◽  
Control Group ◽  
Preventive Strategy ◽  
Grade School ◽  
Knowledge And Practice ◽  
Back Care

Early back care has become the preventive strategy to mitigate bad postural habits and musculoskeletal alterations that trigger inadequate postural patterns in the body schema. The objective was to determine the knowledge and practice of back care in first-grade school children after applying an educational intervention for back care. Quasi-experimental study with pre-test and post-tests in a sample of 71 first grade school students. Knowledge and practices for back care were evaluated before and after of the intervention. During 5 weeks, a program of education for back care was developed in the intervention group, formed by concepts about anatomy, physiology, alterations of the spine, adoption of appropriate postures and movements in school life and the execution of adequate movements learned. Simultaneously, physical exercises based on aerobic work, strengthening and stretching the back muscles were carried out with the children in the control group. A linear regression model and a two-level hierarchical model were applied to estimate the effect of the intervention. After the execution of the back care education program, a better score was found in the knowledge and practice questionnaire, which was different between the intervention group and the control group (1.72 95% CI 1.21-2.24). The development of an education program generated a change in the score of the questionnaire on knowledge of back care in the intervnetion group, which suggests the implementation of these strategies in the school context during early childhood, contributing to the prevention of back disorders and deficiencies

One-generation reproduction toxicity study of Dithane M-45 (mancozeb) and lead acetate

2002 ◽  
Vol 50 (3) ◽  
pp. 365-371 ◽  
Author(s):  
L. Várnagy ◽  
P. Budai ◽  
E. Molnár ◽  
Keyword(s):  
Body Weight ◽  
Lead Acetate ◽  
Clinical Symptoms ◽  
Reproductive Toxicity ◽  
The Body ◽  
High Dose ◽  
Lactation Period ◽  
Treatment Groups ◽  
Body Weight Increase ◽  
Dose Levels

The reproductive toxicity of lead acetate and of a fungicide formulation (Dithane M-45) containing 80% mancozeb was studied on rats. Lead acetate was applied in the feed in the following dose groups: control, 1,000, 5,000 and 10,000 mg/kg of diet. The three treatment groups received, in addition to the above doses of lead acetate, 4,500 mg/kg Dithane M-45 in the diet. The method was based on the OECD Guideline for Testing of Chemicals No. 415 (1981). Clinical symptoms and mortality were not found in the parent generation. The body weight of female animals decreased significantly before the pregnancy period. This tendency was also seen in males after the combination treatment. At the two high dose levels a remarkable body weight increase was seen in the female animals during the lactation period. As a result of treatment, decreased body weight of offspring was measured during the lactation period. No gross pathological changes were seen. Histological examination showed general tubulonephrosis in the experimental animals. It can be established that the administration of Dithane M-45 did not enhance the reproductive toxicity of lead acetate.

scholarly journals Aeromonas veronii Infection Associated with High Morbidity and Mortality in Farmed European Seabass Dicentrarchus labrax in the Aegean Sea, Greece

2017 ◽  
Vol 52 (2) ◽  
pp. 68-81 ◽  
Author(s):  
Maria Smyrli ◽  
Athanasios Prapas ◽  
George Rigos ◽  
Constantina Kokkari ◽  
Michail Pavlidis ◽  
...  
Keyword(s):  
Dicentrarchus Labrax ◽  
Aegean Sea ◽  
Morbidity And Mortality ◽  
High Morbidity ◽  
Aeromonas Veronii ◽  
European Seabass

scholarly journals DIAGNOSIS OF ORAL MUCOSAL PATHOLOGY IN PATIENTS WITH VARIOUS MANIFESTATIONS OF GROUP B VITAMIN IMBALANCE

2021 ◽  
Vol 16 (4) ◽  
pp. 77-83
Author(s):  
Anna Yatsenko ◽  
Lidiya Trankovskaya ◽  
Olga Artyulova
Keyword(s):  
Oral Cavity ◽  
Oral Mucosa ◽  
Human Body ◽  
Clinical Symptoms ◽  
Negative Impact ◽  
Vitamin B12 Deficiency ◽  
Vitamin Deficiency ◽  
The Body ◽  
B Vitamins ◽  
Group B

Subject. The scientific works of recent years show an increase in the degree of negative impact of vitamin deficiency and vitamin-like substances on the state of health of the population. With the deficiency of most vitamins, synthetic processes and regeneration of oral tissues are reduced, so often the initial signs of hypovitaminosis are stomatitis, gingivitis, glossitis, and therefore, it is dentists who are the first to diagnose deviations in the body associated with vitamin deficiency. This justifies the relevance and practical value of studying and describing clinical cases of manifestations of deficient vitamin conditions in the oral cavity. The object – is to study the effect of B vitamins on the oral mucosa in order to increase the effectiveness of diagnosis of vitamin-deficient conditions of the human body. Methodology. These clinical examples illustrate the management experience of patients with manifestations of deficient vitamin conditions on the oral mucosa. Clinical and laboratory methods of diagnosing the analysed conditions of the organism were applied. Statistical processing of materials was carried out using the STATISTICA 10 software (StatSoft, Inc., USA). Results. The deficient condition of the organism in relation to vitamins B2, B6, B12 in patients 18-75 years old has been studied. Characteristic clinical changes on the oral mucosa of the examined patients were established. So, in most patients with vitamin B2 deficiency, the classic Sebrel triad was found: dermatitis, glossitis, cheilitis. In those examined with a lack of vitamin B6, language desquamations (smoothed, polished language) were determined in the 83.6%, often combined with glossodinia. Patients with vitamin B12 deficiency were characterized by a lesion in the form of Meller-Gunter glossitis in 74.9% of cases, moreover, 67.6% of patients showed paresthesia in the area of tongue and oral mucosa. Conclusions. The study found that the first clinical symptoms of deficient conditions of the presented vitamins of group B were found from the oral cavity. Thus, it is the dentist who is the first to diagnose the pathological states of lack of group B vitamins in the human body, which emphasizes the importance and relevance of continuing to study these states of the body.

scholarly journals Diagnostic significance of clinical and para-clinical signs of community-acquired pneumonia in children

2020 ◽  
Vol 24 (2) ◽  
pp. 244-249
Author(s):  
V.Yu. Pasik
Keyword(s):  
Young Children ◽  
Sensitivity And Specificity ◽  
Clinical Symptoms ◽  
Clinical Signs ◽  
The Body ◽  
Diagnostic Methods ◽  
Statistical Hypothesis ◽  
Diagnostic Significance ◽  
Operational Characteristics ◽  
Gallbladder Dyskinesia

Annotation. Respiratory diseases are relevant in pediatric practice, which is associated with its widespread and frequent complications, especially in young children. The aim of the study was to assess the diagnostic value of clinical symptoms, laboratory and ultra-sonographic parameters in pneumonia in children of the first 3 years of life. A retrospective study of medical records of 218 children who were hospitalized in the department for young children diagnosed with pneumonia for the period from 2016 to 2018. The average age of children was 11.67±9.97 months and it was within the range from 1 month to 3 years. The ration of boys and girls was practically identical (51.8% and 48.2% accordingly). The first group included children aged under one year (the average age is 4.57±0.84 months; n=88). The second group included children aged from 1 to 3 years (the average age is 18.2±4.25 months; n=130). To characterize the information content of clinical and laboratory symptoms the study has used objective parameters defined as the operational characteristics of tests. The most important operational characteristics of diagnostic methods included: sensitivity (Se, sensitivity) and specificity (Sp, specificity). To check the statistical hypothesis on differences of absolute and relative frequencies, fractions, and ratios in two independent samples, the criteria of хі-square (χ2) was used. While detailing an anamnesis, the disease was more often related to untimely treatment and outpatient care. Various data were obtained on the absolute and relative risk, as well as the sensitivity and specificity of the localization of pneumonia depending on age. Therefore, the incidence of bilateral pneumonia was considered an indicator of risk. On admission to hospital, the body temperature of patients was 38.2±0.66°С. Most of the complaints were on the unproductive or productive cough. Besides, in some cases, shortness of breath and runny nose were mentioned. Thus, in young children with pneumonia, a diagnostically significant clinical symptom is a bilateral lung impression (82.6%), compared with right-handed (15.1%) and left-handed (2.3%), which is significantly more common in children under 1-th year of life compared with patients 1–3 years; laboratory features are probably higher levels of liver-specific enzymes – ALT and AST in children under 1 year; ultrasonographic indicators associated with the presence of pneumonia in young children include increased liver size, gallbladder deformity, the presence of sediment in the gallbladder, dyskinesia of the biliary tract, thickening of the gallbladder wall; children under 1 year of age have a risk of liver enlargement and biliary dyskinesia.

scholarly journals ЧУТЛИВІСТЬ ЗБУДНИКІВ БАКТЕРІАЛЬНИХ ХВОРОБ ПТИЦІ ДО АНТИБАКТЕРІАЛЬНИХ ПРЕПАРАТІВ

2016 ◽  
Vol 18 (3(71)) ◽  
pp. 182-185
Author(s):  
A.A. Fotina ◽  
Zh.E. Klischova
Keyword(s):  
Bacterial Infections ◽  
Antimicrobial Agents ◽  
Age Groups ◽  
Economic Loss ◽  
Diffusion Method ◽  
Antimicrobial Drugs ◽  
Bacterial Diseases ◽  
Test Systems ◽  
Poultry Farms ◽  
Pathological Material

The using of antibiotics and antimicrobials drugs without control may leads to the development of numerous complications and resistance of microorganisms to antibiotics. The using of antibiotics and antimicrobials drugs should are controlled on poultry farms. That is why the monitoring and determination of sensitivity of bacterial diseases agents to antimicrobial drugs are very important. Results of salmonellas’ and kolibakterias’ monitoring in poultry’s farms of Ukraine are introduced in the article. Researches were conducted at the Department of veterinary sanitary examination, microbiology, zoohygiene and safety and quality of livestock products of Sumy NAU. Sampling for microbiological studies was conducted from the hatchery and from pathological material and premises where poultry of different age groups was held. The spread of the disease, morbidity, mortality, mortality rate, age characteristics, economic loss what diseases cause to the poultry farms were counted. Identification of Salmonella and Escherichia was conducted by ELISA with using of RIDASCREEN® and LOCATE® test systems, according to methodical recommendations of RIDASCREEN® and LOCATE® test systems using. The results were read visually or after addition of storageco with ELISA–photometer (reader) at 450 nm. Sensitivity to antibiotics was determined by disco – diffusion method in agar. Microbiological monitoring of a number of poultry farms in Ukraine has shown that agents of bacterial diseases’ are widely spread. Between the isolated microflora largest number were accounted for Salmonella (54.1%) and the Escherichia (30.8 per cent). The rest (15,1%) were isolated cultures of Proteus, Pseudomonas, Klebsiella, Salmonella, Campylobacteria, Enterobacteria, and Clostridia Citrobacter. This indicates that systematic control over the availability of the causative agents of bacterial infections in all critical points of production of poultry products is very necessary. Among isolates that were isolated from ill poultry and poultry objects, differences in their sensitivity to antimicrobial agents from active substances that officially have registered in our country were discovered. Bactericidal activity of relatively isolated cultures was showed by colistin, ftorfenicol, zeftiocur, TimTil 250, doxicyclin, enroxil and sarafloxacin.  

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