INFLUENCE OF IMMUNOSTIMULATORS ON MORPHOLOGICAL AND IMMUNOBIOCHEMICAL PARAMETERS OF BLOOD OF PREGNANT COWS AND NEWBORN CALVES

2020 ◽  
Vol 36 (2) ◽  
pp. 215-223
Author(s):  
O.M. Altynbekov ◽  
◽  
A.V. Andreeva ◽  
Keyword(s):  
Red Blood Cells ◽  
Total Protein ◽  
Blood Cells ◽  
Gamma Globulin ◽  
White Blood Cells ◽  
Blood Parameters ◽  
Control Group ◽  
Maximum Difference ◽  
Black And White ◽  
Newborn Calves

The article reflects the results of changes in the biochemical and morphological parameters of blood, as well as the content of total protein and its fractions under the influence of immunostimulators in black-and-white cows and calves obtained from them. The aim of the study was to study the effect of the immunostimulators “Recombinant Bovine Interferon” and “Immunate” on the morphological and immunobiochemical blood parameters of pregnant cows and newborn calves. Studies were conducted on 45 pregnant cows divided into three groups based on the principle of analogues, and, subsequently, on 45 calves obtained from mother cows and divided into nine groups. Maternal cows during pregnancy and newborn calves were given immunostimulators in various combinations. In the blood of cows of the experimental groups, five days before calving, higher indicators of red blood cells and hemoglobin were registered in comparison with the control indicators. Also, in experimental cows, the content of total protein and globulins exceeded the indicators of the control group. Calves received from cows immunostimulated with “Recombinant Bovine Interferon” and who were given “Immunate” after birth had the smallest decrease in the number of red blood cells and white blood cells on the tenth day. After drinking colostrum in calves of experimental groups, the total amount of protein increased, especially there was an increase in the gamma-globulin fraction. For all periods of the study, the content of immunoglobulins A, M, G in the blood serum of calves of the control group was lower than that of calves of the experimental groups. The maximum difference between these indicators and the control was found in animals of the sixth and ninth groups.

scholarly journals Effect of dietary phytoextracts supplementation on growth performance and blood parameters of rainbow trout ( Oncorhynchus Mykiss W. ), cultivated in a recirculation system

2018 ◽  
Vol 16 (4) ◽  
pp. 292-299
Author(s):  
K. Georgieva ◽  
G. Zhelyazkov
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Red Blood Cells ◽  
Growth Performance ◽  
Total Protein ◽  
Blood Cells ◽  
White Blood Cells ◽  
Blood Parameters ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Recirculation System

The present research aimed to examine the effect of dietary phytoextracts supplementation on the growth performance, haematological (white blood cells, red blood cells, hemoglobin, hematocrit) and biochemical (glucose, urea, creatinine, total protein, albumin, ASAT, ALAT) blood parameters of rainbow trout (Oncorhynchus mykiss W.), cultivated in a recirculation system. The fish were divided into 6 groups: one control (C) and five experimental groups in whose food was added phytoextracts of curcumin (EC), paprika (EP), thyme (ET), oregano (EO) and garlic (EG). The inclusion of phytoextracts had no significant effect on growth parameters of fish from EC, EP, ET, EO and EG groups (P>0.05). Statistically significantly lower feed consumption per unit weight gain was observed in EO group vs C (Р<0.05). The phytoextract supplementation had significant influence on some of the haematological (white blood cells, red blood cells, hemoglobin) and biochemical (urea, creatinine, total protein, albumin, ASAT, ALAT) blood parameters of rainbow trout.

Hematological condition of breeding bulls after a long winter period of operation in the conditions of the Udmurt Republic

2022 ◽  
pp. 67-73
Author(s):  
A. Abilov ◽  
A. Azhmyakov ◽  
I. Novgorodova ◽  
N. Bogolyubova
Keyword(s):  
Red Blood Cells ◽  
Reference Values ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Winter Period ◽  
Black And White ◽  
Significant Difference ◽  
White Breed ◽  
Udmurt Republic

Purpose: to study hematological parameters of blood in bulls-producers of dairy breeds on the day of semen collection in the Udmurt Republic after a long winter period of operation, depending on the breeds, age and place of selection.Materials and methods. The work was performed at the Federal Research Center for Animal Husbandry named after Academy Member L. K. Ernst on the basis of AO "Udmurtplem" of the Udmurt Republic in the period from 2020 to 2021 on dairy bulls (n=20) aged 15-69 months, including a purebred Holstein breed of domestic selection (n=6), a Holstein black-and-white breed of European selection (the Netherlands, n=6), a black-and-white breed with blood transfusion on Holsteins on at the level of 94-98% (n=8). The content of white blood cells, red blood cells, hemoglobin and hematocrit, depending on age and breed, was studied on the ABC VET hematological analyzer on the day of taking the seed.Results. It was found that on average, in 20 bulls aged 15-69 months, the level of white blood cells was at the level of reference values of 8.8±0.25 x 109/l, red blood cells 10.3 x 1012/l, which is 50% more than the reference values. The concentration of hemoglobin is 128.0±2.92 g/l, hematocrit is on average 54.3%, with a norm of 24-46%. Breeding bulls at a reliable level, differing in age, showed that some animals had high indicators for red blood cells of 10.3±0.26 x 102, for hematocrit of 54.3% against 24-46% of reference values. Also, according to hematological indicators, there was a tendency to increase the concentration of hemoglobin and hematocrit in European-bred bulls.Conclusion. The study of the variability in hematological parameters depending on the selection showed that there is no significant difference in leukocytes and all indicators are at the level of reference values, and in erythrocytes more than 50% than the highest indicators. The highest hemoglobin values were at the level of 141-156 g/l instead of 128 g/l according to the highest reference values. Hematocrit also showed high max values in all groups in comparison with the reference values of 59-66% versus 46% in the norm. It is necessary when analyzing hematological blood parameters in addition to the average statistical indicators (M+m) also, monitor the variability (min-max) in order to obtain more objective information.

scholarly journals BIOLOGICAL EFFECTS OF SILICON OXIDE NANOPARTICLES ON BROILER CHICKEN

2020 ◽  
Vol 5 (2) ◽  
pp. 63-71
Author(s):  
Vladimir Nikulin ◽  
Aleksandra Mustafina
Keyword(s):  
Red Blood Cells ◽  
Total Protein ◽  
Blood Cells ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Silicon Oxide ◽  
The Body ◽  
Control Group ◽  
The Third ◽  
Experimental Group

The aim of the study is to increase the productive qualities of broiler chickens by including ultrafine silicon oxide into main diet. During the experiment, the biological effect of ultrafine silicon oxide on broiler chickens was estab-lished. Use of ultrafine SiO2 particles for poultry feeding contributed to an increase in the number of red blood cells and content of total protein and albumins. By the end of the experiment, the number of red blood cells in birds in-creased by 17.43% (P≤0.001) – in the blood of birds of the first experimental group, 16.51% (P≤0.01) – the second one, 20.80% (P≤0.001) – the third experimental and 21.71% (P≤0.001) – the fourth experimental group, compared with the indicator of the control group. The amount of total protein in blood serum of chickens of the first and the second experimental groups increased by 1.36-1.39 %, in the third and fourth ones there was a significant (P≤0.05) increase by 5.45 and 3.05%, respectively. The blood glucose content of chickens in the experimental groups is higher by 8.04-23.65% compared to this indicator with ones in the control group. During the experiment feed con-sumption per 1 kg gain of live weight decreased: in the first experimental group by 3.00 % in the second by – 0.50 %, the third – 6.00 % the fourth– by 4.50 %, compared to this with the control group. The chicken’s vibrancy of the first the experimental group was higher by 4.77 %, the second – by 6.20 %, the third – by 19.25 % and the fourth– by 11.59% than in the control one. Consequently, when converting the feed energy into the body energy of a broiler chicken, the energy conversion coefficient of the experimental group of poultry is higher than that of the control one by 7.16-21.76 %. Thus, the most optimal dose for further research was determined.

scholarly journals The Effect of Experimental Escherichia coli Infection on Some Blood Parameters and Histological Changes in Male Rats

2011 ◽  
Vol 35 (2) ◽  
pp. 22-27
Author(s):  
Hayder AL – Zamely
Keyword(s):  
Escherichia Coli ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Histopathological Examination ◽  
Hemoglobin Concentration ◽  
Blood Parameters ◽  
Infected Group ◽  
Male Rats ◽  
Control Group ◽  
Renal Tubules

This study was carried out to investigate the effect of experimental infection by Escherichia coli on some blood parameters and histopathological changes in male rats to reach this objective twenty male rats (Rattus norvigicus) were divided into two groups, housed under same conditions of water, feed, temperature, light and humidity. The first one served as control group, while the second was experimentally infected with Ecoli by administration of 2ml / rat of (1×109) colony forming unit/ml of E-coli. Five days later blood were obtained from animals by cardiac puncture to measure the total and differential white blood cells count hemoglobin concentration packed cells volume red blood cells count, and thrombocytes count. One animal from each group was killed and histopathological examination was done for intestine kidney liver and spleen to detect the effect of infection on these organs. The results revealed a significant increase in total leukocytes count (P<005) in infected group while the differential count showed a significant increase in neutrophils and significant decrease in lymphocytes (P< 0.05) in same group. There were a significant decrease in hemoglobin concentration packed cell volume and non significant decrease in red blood cells count. While thrombocytes count showed a significant decrease in infected group compared with control group (P<0.05). The histopathological examination of infected male rats intestine was demonstrated mild diffuse inflammation which include increased numbers of neutrophile in tissue intraepithelial lymphocytes and goblet cells In kidney renal tubules showed extensive epithelial swelling with decreased lumen space and generalized necrotic changes with interstitial hemorrhage in renal cortex. In liver hepatocytes were swollen with decreased sinusoidal spaces and widely distributed necrotic foci were seen. There is extensive splenic necrosis with infiltration of leukocytes from white pulp to red pulp of spleen.

scholarly journals The effect of colostral supplement on the serum protein fractions, health status and growth of calves

2011 ◽  
Vol 54 (2) ◽  
pp. 115-126 ◽  
Author(s):  
M. Szewczuk ◽  
E. Czerniawska-Piątkowska ◽  
S. Palewski
Keyword(s):  
Total Protein ◽  
Gamma Globulin ◽  
Poor Quality ◽  
Favourable Effect ◽  
Control Group ◽  
Health State ◽  
Globulin Fraction ◽  
Black And White ◽  
Time Period ◽  
Holstein Friesian

Abstract. The study aimed at evaluation of the effect of colostral supplement on humoral immunity, growth and health state of calves until the 3rd month of life. Examinations were carried out on 40 Polish Black-and-White Holstein-Friesian calves. After birth, the calves were separated into two groups (20 calves each): control group – without addition of colostral supplement, and experimental one – with colostral supplement in diet. Blood was sampled from each calf on day 5 and 30 of life, and total protein, albumin, and alpha, beta and gamma globulin levels were determined in it. Evaluation of calves’ growth and development course was carried out basing on weighing and daily gain results. The favourable effect of colostral supplement on calves’ health state and body weight gains was found. No significant effect of colostral supplement on total protein level or gamma-globulin fraction level was found. The effect of colostral supplement on reduction of alpha-globulin fraction decrease was found in a time period until the 30th day of calves’ life (P≤0.01). The study so far point to the purposefulness of using this supplement, in particular in the case of colostrum deficiency or its poor quality.

Preliminary results of the study on some hematological and biochemical values of Takhi

2014 ◽  
Vol 11 (2) ◽  
pp. 3-6
Author(s):  
B Bayar-Enkh ◽  
S Ganbat ◽  
P Enkhtuya
Keyword(s):  
Red Blood Cells ◽  
Whole Blood ◽  
Total Protein ◽  
Blood Cells ◽  
White Blood Cells ◽  
Total Proteins ◽  
Preliminary Results ◽  
Natural Park ◽  
Calcium And Phosphorus ◽  
Hematological Values

Some hematological values, blood total protein, protein fractions, and minerals such as calcium and phosphorus of takhi, which are now being reintroduced in Khustain natural park, were measured. There were 8.3 million red blood cells and 8000 white blood cells in 1 mm3 whole blood of takhi reintroduced in Khustain natural park, and hemoglobin was 179.6±3.2 g/l. As well, total proteins, calcium and phosphorus of takhi were 67± 0.6 g/l, 2.1±0.31 mmol/l, and 1.1±0.2 mmol/l respectively and they were similar to those in Mongolian horses. It has been necessary to investigate further adaptability of takhi in asscoation with both internal and external environments of its body.DOI: http://dx.doi.org/10.5564/mjas.v11i2.206 Mongolian Journal of Agricultural Sciences Vol.11(2) 2013 pp.3-6

scholarly journals RELATIONSHIP OF LAMB INTERIOR INDICATORS OF DIFFERENT GENOTYPES WITH THEIR PRODUCTIVITY

2021 ◽  
Vol 6 (1) ◽  
pp. 32-38
Author(s):  
Khamidulla Baymishev ◽  
Baluash Traisov ◽  
Murat Baymishev ◽  
Kayrly Esengaliev
Keyword(s):  
Red Blood Cells ◽  
Total Protein ◽  
Blood Cells ◽  
Live Weight ◽  
Blood Parameters ◽  
The North ◽  
Growth Intensity ◽  
Group 3 ◽  
Relationship Of ◽  
Group 1

The aim of the research is increasing the productive indicators of replacement sheep of different genotypes by im-proving the interior indicators. To conduct the research, three groups of ewe hoggs with 20 heads each were formed: 1 group – ewe hoggs born from stud rams and Akzhaik ewes (AKSHM x AKSHM); 2 group – ewe hoggs born from stud rams of the North Caucasian breed and Akzhaik ewes (SK x AKSHM); 3 group – ewe hoggs born from Kuibyshev stud ram breed and Akzhaik ewes (KB x AKSHM). Blood parameters, live weight at the age of 4 and 8 months, hair cut at the age of 8 months was studied of animals of these groups. It was found that blood parame-ters of the ewe hoggs of different genotypes have significant differences and these affect the indicators of growth intensity and hair cutting. The content of red blood cells and hemoglobin of ewe hoggs from group 3 was higher by 0.551012/l and 9.32 g/l, respectively, than of ones from group 1. The biochemical blood parameters of crossbred ewe hoggs from groups 3 and 2 at 4 and 8 months of age in terms of total protein by 2.84 and 4.73 g/l, glucose – by 0.86 and 1.02 mmol/l exceeded the results of purebred Akzhaik ewe hoggs. Crossbreds of groups 2 and 3 in the 8-month age were superior to their herdmates of Akzhaik breed in live weight by 3.04 and 2.74 kg, and the washed wool cut – by 0.18 and 0.24 kg, respectively.

scholarly journals Physiological status of broiler chickens with diets supplemented with milk thistle extract

2021 ◽  
pp. 1319-1323
Author(s):  
Olga Bagno ◽  
Sergey Shevchenko ◽  
Antonina Shevchenko ◽  
Oleg Prokhorov ◽  
Anna Shentseva ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Broiler Chickens ◽  
Clinical Chemistry ◽  
Current Trend ◽  
White Blood Cells ◽  
Control Group ◽  
Physiological Status ◽  
Milk Thistle ◽  
Wing Vein

Background and Aim: In recent decades, the use of various feed supplements is the current trend in poultry farming, among which phytogenics serve as alternatives to feed antibiotics. This study aimed to examine the effect of feeding various doses of milk thistle extract (Silybum marianum) on the morphological and biochemical parameters of the blood in broiler chickens. Materials and Methods: Experiments were carried out in an industrial poultry farm on broiler chickens of the Hubbard ISA F15 cross for 40 days. One control group and five experimental groups of day-old chickens were formed. The number of birds in each group was 50. Broilers of all groups received complete feed, and the experimental groups received an additional milk thistle extract at doses of 0.1, 0.5, 1.0, 1.5, and 2.0 mg/kg of body weight. Milk thistle medicinal plant extract was obtained using water-ethanol extraction followed by low-temperature vacuum drying. For the assessment of blood analyses, samples were collected from the wing vein of six chickens per group. Using unified methods recommended by the International Federation of Clinical Chemistry, the content of red blood cells, hemoglobin, white blood cells, total protein, protein fractions, triglycerides, glucose, calcium, phosphorus, and the concentration of alanine aminotransferase and aspartate aminotransferase in the blood serum were determined. Results: It was found that the introduction of milk thistle extract into the diet of broiler chickens with the aforementioned doses increased the number of red blood cells, hemoglobin, white blood cells in the blood, as well as a decrease in the level of albumin and an increase in the content of γ-globulins in its serum. Conclusion: The authors assume that the introduction of milk thistle extract into a complete feed for broiler chickens increased the anabolic processes in their bodies, accompanied by increased use of proteins of the albumin fraction as the main material for organogenesis.

THE INFLUENCE OF ENDOMETRIOID HETEROTOPIAS ON THE RED BLOOD PARAMETERS

2020 ◽  
Vol 65 (7) ◽  
pp. 424-427
Author(s):  
R. V. Ukrainets ◽  
Yulia Sergeevna Korneva
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Blood Parameters ◽  
Blood Analysis ◽  
Ca 125 ◽  
Differential Diagnostics ◽  
Ovarian Cysts ◽  
Control Group ◽  
Before And After ◽  
Medical Histories

Endometrioid heterotopia can have an adverse systemic effect on the organism through erythropoietin synthesis, which can affect erythropoiesis and reflect in blood analysis. The aim of the study was to investigate the effect of endometrioid heterotopia on parameters of red blood. The investigated group included patients with endometrioid heterotopias of various localisations (115 cases), functional ovarian cysts made up the control group (28 cases). Retrospectively, the number of red blood cells, the amount of hemoglobin, and the level of (CA-125) were recorded in the medical histories. The parameters were taken into account both before and after radical surgery. The highest values of the number of red blood cells and hemoglobin are observed in endometrioid ovarian cysts, followed by adenomyosis, endometriosis of the pelvic peritoneum and skin scar, respectively. After surgery, these differences have disappeared. Comparison of the number of red blood cells and hemoglobin before and after surgery revealed significant changes for both parameters in the group of patients with endometriosis, while in the control group the number of red blood cells and the level of hemoglobin did not change significantly. The number of cases with endometrioid ovarian cysts, where the red blood cell count was higher than normal, significantly differed from the control group; on the contrary, with non-endometrioid ovarian cysts, a significantly more frequent decreased number of red blood cells was observed. In addition, red blood counts in patients with endometriosis before surgery had a moderate negative correlation with the level of CA-125 protein.The possible systemic influence of endometrioid heterotopias on erythropoiesis in the form of its stimulation is demonstrated. In non-endometrioid ovarian cysts a significantly more frequent decrease in the number of red blood cells is observed. The reveales pecularities can be used for complex differential diagnostics of ovarian cysts at the preoperative stage due to their easy accessibility and minimally invasive nature.

scholarly journals Evaluation of Hematocrit Level, Red Blood Cells and White Blood Cells Counts in Blood from Patients with Different Severities of Periodontal Diseases

2018 ◽  
Vol 30 (3) ◽  
pp. 1-6
Author(s):  
Chinar J Ali ◽  
Maha A Ahmed
Keyword(s):  
Chronic Disease ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Periodontal Diseases ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Control Group ◽  
Pocket Depth ◽  
Anemia Of Chronic Disease ◽  
Mean Values

Background: Anemia of chronic disease (ACD) occurs in the presence of chronic infection, inflammatory conditions or neoplastic conditions despite of adequate iron and vitamins storage. Gingivitis is the inflammation of the gingiva, periodontitis is the inflammation in the periodontium that extend deeper with loss of connective tissue attachment and supporting bone. The main pathogenesis of periodontal diseases and ACD is immune activation. Aims of study: Determine and compare the clinical periodontal parameters (plaque index (PLI), gingival index (GI), bleeding on probing (BOP), probing pocket depth (PPD) and clinical attachment level (CAL)). Evaluate the hematocrit (Hct) level, red blood cells (RBCs) count and white blood cells (WBCs) count. Assess the correlations between the clinical periodontal parameters and hematological parameters at patients had gingivitis, chronic periodontitis (CP) with different severities (mild, moderate and severe) with healthy periodontium subjects. Materials and Methods: 35-50 years old, 150 male subjects were included in this study. They were divided into three study groups: group of 30 patients with gingivitis, group of 90 patients with CP which subdivided into (Mild CP=30 patients, Moderate CP =30 patients, Severe CP =30 patients) and control group 30 subjects with clinically healthy periodontium. Blood samples were collected then by automated blood analyzer the Hct, RBCs and WBCs were evaluated. Results: Comparisons among groups and subgroups revealed significant differences in Hct and WBCs, while RBCs was non-significant. Means values of RBCs count showed reduction in mild and severe CP subgroups. while, the Hct and WBCs mean values increased in patients with periodontal disease. The correlations between the clinical periodontal parameters with WBCs and RBCs were almost non-significant but, with Hct was mostly significant negative correlations. Conclusion: Inflammatory and immune responses in periodontal diseases caused change in different hematologic parameters which could contribute to the development of anemia of chronic disease.

Sign in / Sign up

Export Citation Format

Share Document