Quercus infectoria gall extract aids wound healing in a streptozocin-induced diabetic mouse model

2021 ◽  
Vol 30 (8) ◽  
pp. 618-625
Author(s):  
Farhad Dardmah ◽  
Mohammad Reza Farahpour
Keyword(s):  
Wound Healing ◽  
Mouse Model ◽  
Antioxidant Capacity ◽  
Control Group ◽  
Open Wound ◽  
Wound Area ◽  
Tnf Α ◽  
Hydroethanolic Extract ◽  
Quercus Infectoria ◽  
P53 Mrna

Objective: Quercus infectoria galls have commonly been used for different therapeutic purposes. This study was conducted to investigate the effects of topical application of an ointment prepared from Quercus infectoria gall hydroethanolic extract on open wound healing in a streptozocin-induced diabetic BALB/c mouse model. Method: After induction of diabetes, two circular wounds (5mm) were created on the dorsum of the mice which were then divided into three groups. The mice were treated with soft yellow paraffin (control-sham group) and therapeutic doses of 5% and 10% of an ointment prepared from Quercus infectoria, respectively. To evaluate the effects of the therapeutic ointment on the wound healing process, wound area, histological parameters, mRNA levels of vascular endothelial growth factor (VEGF), Bcl-2 and p53, plasma levels of interleukin-6 (IL-6) and tumour necrosis factor (TNF)-α, and tissue antioxidant capacity were investigated. Results: The mice (n=54) were divided into three equal groups. Wound area and concentrations of IL-6 and TNF-α were significantly decreased in both ointment-treated groups compared to the control group (p<0.05). Moreover, angiogenesis, fibroblast distribution per mm2 of wound tissue, collagen deposition, rapid re-epithelialisation, and the expression of VEGF, Bcl-2 and p53 mRNA, were significantly increased (p<0.05). The administration of the ointment reduced malondialdehyde concentration and increased total antioxidant capacity compared with the control group (p<0.05). Conclusion: Our study suggests that an ointment prepared from Quercus infectoria gall hydroethanolic extract accelerated open wound healing in a diabetic animal model by shortening the inflammatory phase, inducing apoptosis, up-regulating the expression of Bcl-2 and p53 mRNA, antioxidant properties and cellular proliferation.

scholarly journals Topical Wound Healing Activity of Myricetin Isolated from Tecomaria capensis v. aurea

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 4870
Author(s):  
Abdelsamed I. Elshamy ◽  
Naglaa M. Ammar ◽  
Heba A. Hassan ◽  
Walaa A. El-Kashak ◽  
Salim S. Al-Rejaie ◽  
...  
Keyword(s):  
Wound Healing ◽  
Inflammatory Cytokines ◽  
Group Treatment ◽  
Wound Closure ◽  
Burn Injury ◽  
Control Group ◽  
Albino Rats ◽  
Large Area ◽  
Blood Capillaries ◽  
Tnf Α

Wounds and burn injury are major causes of death and disability worldwide. Myricetin is a common bioactive flavonoid isolated naturally from the plant kingdom. Herein, a topical application of naturally isolated myricetin from the shoots of Tecomaria capensis v. aurea on excisional wound healing that was performed in albino rats. The wounded rats were treated every day with 10 and 20% myricetin for 14 days. During the experiment, the wound closure percentage was estimated at days 0, 7, and 14. Effects of myricetin on the inflammatory cytokines (tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and cluster of differentiation 68 (CD68) in the serum were evaluated using immunosorbent assay kits. The percentage of wound closure and contraction was delayed in wounded rats (67.35%) and was remarkably increased after treatment of wounded rats with myricetin; the treatment with 20% myricetin was the most potent (98.76%). Histological findings exhibited that 10% myricetin caused the formation of a large area of scarring at the wound enclosure and stratified squamous epithelium without the formation of papillae as in the control group. Treatment with 20% myricetin exhibited less area of scarring at the wound enclosure as well as re-epithelialization with a high density of fibroblasts and blood capillaries in the wound. Level elevations of serum pro-inflammatory cytokines, IL-1β, and TNF-α and macrophage CD68 were decreased in wounded rats treated with myricetin. Thus, it can be suggested that the enhancements in inflammatory cytokines as well as systemic reorganization after myricetin treatment may be recommended to play a crucial part in the promotion of wound healing. The findings suggest that treatment with a higher dose of myricetin was better in improving wound curing in rats. It could serve as a potent anti-inflammatory agent and can be used as an adjunctive or alternative agent in the future.

scholarly journals Local Growth Hormone Therapy for Pressure Ulcer Healing on a Human Skin Mouse Model

2019 ◽  
Vol 20 (17) ◽  
pp. 4157 ◽  
Author(s):  
Lara Cristóbal ◽  
Nerea de los Reyes ◽  
Miguel A. Ortega ◽  
Melchor Álvarez-Mon ◽  
Natalio García-Honduvilla ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Wound Healing ◽  
Mouse Model ◽  
Human Skin ◽  
Pressure Ulcer ◽  
Healing Rate ◽  
Recombinant Human Growth Hormone ◽  
Control Group ◽  
P Value ◽  
Type I

The growth hormone is involved in skin homeostasis and wound healing. We hypothesize whether it is possible to improve pressure ulcer (PU) healing by locally applying the recombinant human growth hormone (rhGH) in a human skin mouse model. Non-obese diabetic/severe combined immunodeficient mice (n = 10) were engrafted with a full-thickness human skin graft. After 60 days with stable grafts, human skin underwent three cycles of ischemia-reperfusion with a compression device to create a PU. Mice were classified into two groups: rhGH treatment group (n = 5) and control group (n = 5). In the rhGH group for local intradermal injections, each had 0.15 mg (0.5IU) applied to the PU edges, once per week for four weeks. Evaluation of the wound healing was conducted with photographic and visual assessments, and histological analysis was performed after complete wound healing. The results showed a healing rate twice as fast in the rhGH group compared to the control group (1.25 ± 0.33 mm2/day versus 0.61 ± 0.27 mm2/day; p-value < 0.05), with a faster healing rate during the first 30 days. The rhGH group showed thicker skin (1953 ± 457 µm versus 1060 ± 208 µm; p-value < 0.05) in the repaired area, with a significant decrease in collagen type I/III ratio at wound closure (62 days, range 60–70). Local administration of the rhGH accelerates PU healing in our model. The rhGH may have a clinical use in pressure ulcer treatment.

scholarly journals Both obesity-prone and obesity-resistant rats present delayed cutaneous wound healing

2011 ◽  
Vol 106 (4) ◽  
pp. 603-611 ◽  
Author(s):  
Adriana Paulino do Nascimento ◽  
Andréa Monte-Alto-Costa
Keyword(s):  
Wound Healing ◽  
Saturated Fat ◽  
Inflammatory Cells ◽  
Male Rats ◽  
Control Group ◽  
Cutaneous Wound Healing ◽  
Cutaneous Wound ◽  
Diet Induced Obesity ◽  
Tnf Α ◽  
Obesogenic Diet

Diet-induced overweight rats exhibit delayed cutaneous healing; however, when receiving an obesogenic diet, some rats are susceptible to developing the overweight phenotype, whereas others are resistant. We investigated cutaneous healing in diet-induced obesity (DIO)-prone and diet-resistant (DR) rats. Male rats were fed with a standard (control) or a high-saturated fat (30 % fat, w/w) diet for 20 weeks. Then, the experimental group was subdivided into DIO (n 17) and DR (n 16) groups. An excision lesion was made, and the animals were killed 7 or 14 d later. The average body weight was 29 and 25 % higher in the DIO group compared with the C and DR groups. Retroperitoneal fat was higher in the DIO group than in the control and DR groups (518 and 92 %) and was higher in the DR group than in the control group (223 %). The DIO group presented glucose intolerance, and both the DIO and DR groups presented delayed wound contraction (50 %) and re-epithelialisation (20 %). Compared with the DR group, the DIO group displayed higher amounts of inflammatory cells as well as higher levels of lipid peroxidation (P < 0·05). Myofibroblastic differentiation and vessel remodelling were delayed in both the DIO and DR groups. Nitrite levels were lower in the DIO group (340 % less) than in the DR group. TNF-α expression was increased in the DIO group (130 %) compared with the DR group. Our results showed that DIO as well as DR rats present delays in cutaneous wound healing, even though the DR group does not have an overweight phenotype.

scholarly journals PENGARUH PENGGUNAAN ASI PADA PERAWATAN LUKA PERINIEM DENGAN LAMA WAKTU PENYEMBUHAN LUKA PERINIUM

2019 ◽  
Vol 7 (2) ◽  
pp. 67-73
Author(s):  
Annisa Ul Mutmainnah ◽  
Siti Noorbaya
Keyword(s):  
Wound Healing ◽  
Data Analysis ◽  
Random Sampling ◽  
Wound Care ◽  
Healing Time ◽  
Natural Process ◽  
Control Group ◽  
P Value ◽  
Research Subjects ◽  
Wound Area

Childbirth is a natural process, but sometimes labor can also cause birth canaltrauma, especially in the aluminum region, this trauma can be injuries to the periniumarea, the cause can be intentional biases such as episiotomy or unintentional actions such as spontaneous tears in the process of removing the baby. Injury to the perineum if not treated properly can cause postpartum infection because the wound area will be a medium for developing germs. The purpose of this study was to analyze the effect of the use of breast milk in the treatment of periniem wounds with the duration of perinium wound healing at the Ramlah Parjib Clinic in Samarinda. The method used in this study was Posttest Only Control Group Desigen research subjects were postpartum motherswith a sample of 60 respondents taken by random sampling and grouped based on treatment using ASI. Data analysis using ANOVA test. The results showed that the use of ASI had a significant effect on the treatment of periniem wounds with a healing time seen from the P value of 0,000. Conclusion: Periniuem wound care techniques are factors that influence the duration of perinieum wound healing

scholarly journals Evaluation of Indonesian and two types of Japanese honey on the acceleration of cutaneous wound healing: an experimental study in mice

2019 ◽  
Vol 2 (2) ◽  
pp. 80-88
Author(s):  
Haryanto Haryanto ◽  
Kanae Mukai ◽  
Nakajima Yukari ◽  
Mayumi Okuwa ◽  
Cau Kim Jiu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
Wound Area ◽  
Hydrocolloid Dressing ◽  
Thickness Skin ◽  
Microscopic Evaluation ◽  
Acacia Honey ◽  
Macroscopic Findings

The purpose of this study is to evaluate and clarify the effect of Indonesian honey, two types of Japanese honey and hydrocolloid dressing on wound healing process. Four groups of male mice were treated to produce two circular full-thickness skin wounds on the dorsum. They were then randomly allocated to receive daily Indonesian honey, Japanese honey (Buckwheat and Acacia honey) or hydrocolloid dressing as a control for treatment application. Macroscopic findings were observed from day 0 to 14 after wounding. Microscopic evaluation was assessed using qualitative analysis. The ratios of wound areas for honey groups on day 3 and 7 were smaller than those of the control group. Wound areas of honey groups gradually decreased to almost the same wound area as the control group on day 14, while the wound area of the control group peaked on day 5 and rapidly decreased until day 14. Microscopic finding that Indonesian honey was different with Japanese honey especially Buckwheat honey.

scholarly journals The Effect of Aloe vera (Linn) On Cutaneous Wound Healing and Wound Contraction Rate in Adult Rabbits

2016 ◽  
Vol 5 (3) ◽  
Author(s):  
Martha Orendu Attah ◽  
Tonye Watson Jacks ◽  
Attah Jacob ◽  
Otong Eduitem ◽  
Barnabas John
Keyword(s):  
Wound Healing ◽  
Aloe Vera ◽  
Wound Contraction ◽  
Control Group ◽  
Cutaneous Wound Healing ◽  
Contraction Rate ◽  
Wound Area ◽  
Cutaneous Wound ◽  
Cutaneous Wounds ◽  
Aloe Vera Gel

Background: In the present research study, the rate of cutaneous wound healing and contraction rate in healthy rabbits using Aloe vera pulp was studied.Methods: Ten healthy rabbits were used for the study. They were divided into two groups consisting of five rabbits each. Cutaneous wounds were made on the lumbar region of each rabbit using a template which ensured that the wounds were of the same size in all the rabbits. 5ml of Aloe vera gel was applied to the wounds of the animals in the test group, while nothing was applied to the wound area of the animals in the control group. The wound area in each group was measured for a period of 21 days, using a venire caliper and tracing paper which was used to trace the wound area. Tissue samples were removed from the wound area in both experimental and control groups and subjected to routine histological analysis, also, morphometric analysis was performed.Results: The rate of wound contraction and mean centripetal contraction was calculated in both groups and graphically represented using Microsoft Excel. The results showed that animals who were treated with Aloe vera gel had a greater wound contraction rate, as well as rapid wound closure. The micrographs showed a thicker epithelial layer, with thinner collagen fibers in the dermis of experimental animals compared to the control group. There was also an abundant capillary bed at the dermal-epidermal junction in the experimental group, compared to the control group.Conclusion:  Aloe vera may increase the rate of wound healing by accelerating epithelial migration, and may also play a role in neo-vascularization of the newly healed area.Keywords: Aloe vera, Cutaneous Wounds, Rabbits, Wound Contraction, Wound Healing

scholarly journals Topical ozonated virgin coconut oil improves wound healing and increases HSP90α, VEGF-A, EGF, bFGF, and CD34 in diabetic ulcer mouse model of wound healing

F1000Research ◽  
2021 ◽  
Vol 9 ◽  
pp. 580
Author(s):  
Renni Yuniati ◽  
Prasetyowati Subchan ◽  
Wibi Riawan ◽  
Matthew Brian Khrisna ◽  
Maryam Restiwijaya ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mouse Model ◽  
Healing Process ◽  
Coconut Oil ◽  
Control Group ◽  
Wound Healing Process ◽  
Virgin Coconut Oil ◽  
Diabetic Ulcer ◽  
Macroscopic Appearance ◽  
Wound Length

Background: Diabetes is a disease that affects people worldwide, including in Indonesia. The prevalence of diabetes in Indonesia is increasing from year to year. One of the most devastating complications of diabetes mellitus is diabetic ulcers, which is a limb-threatening complication. Over the past few decades, ozone generated using plasma medical technology has been investigated as an agent that helps wound healing. This study aims to evaluate the effects of topical ozonated virgin coconut oil (VCO) in a diabetic wound mouse model. Methods: This study was an experimental study with a post-test control design. An ulcer wound model was made in 50 diabetic male Wistar mice, divided into five groups, and a control group of 10 non-diabetic mice. The control groups were given conventional therapy only and the treatment groups were also given topical ozonated VCO with different flow durations (0 min, 90 min, 7 h, 14 h). Macroscopic appearance and wound contraction were observed. HSP90β, VEGF-A, EGF, bFGF, and CD34 levels were measured from the immunostained slices of wound margins. Results: The reduction of wound length was proportionally related to the duration of ozone flow. Ozonated VCO with a longer duration of ozone flow healed the wound more quickly and had the shortest wound length. VCO with ozone flow for 14 hours (16837.10 µm) had the biggest reduction in wound length compared to other groups. The wounds treated with ozonated VCO showed an increase in HSP90β, VEGF-A, EGF, bFGF, and CD34 levels that correlated to improved wound healing. A longer period of treatment resulted in higher levels of wound healing biomarkers compared to shorter therapeutic durations. Conclusions: Topical ozonated VCO improved the wound healing process in a diabetic ulcer mouse model by improving macroscopic wound appearance and increasing levels of wound healing biomarkers.

scholarly journals Wound-healing effect of Platycodon grandiflorus (Jacq.) extract in rats

2021 ◽  
Vol 18 (9) ◽  
pp. 1909-1912
Author(s):  
Liang Wang ◽  
Li Yang ◽  
Gang Yuan ◽  
Long-Jiao Hu
Keyword(s):  
Wound Healing ◽  
Treated Group ◽  
Control Group ◽  
Type Iii Collagen ◽  
Matrix Metalloprotease ◽  
Wound Area ◽  
Burn Wounds ◽  
Type Iii ◽  
Healing Effect ◽  
Wound Tissue

Purpose: To evaluate the healing effect of Platycodon grandiflorus (Jacq.). extract (PGE) on experimental burn wounds in rats. Methods: Rats were randomly divided into four groups of eight rats each: control group, silver sulfadiazine (SSD)-treated group, moist exposed burn ointment (MEBO)-treated group and PGE-treated group. PGE, SSD and MEBO were applied topically twice daily for 7 days. SSD and MEBO were used as reference control. External observation of wound area contraction and histological analysis of wound tissues was performed respectively. The effect of PGE on matrix metalloproteases (MMPs), vascular endothelial growth factor (VEGF) and Type-III Collagen proteins of wound tissue in rats were analysed by Western blot. Results: After 10 days of topical treatment with PGE, PGE-treated group showed faster reduction in wound area when compared with control groups (p < 0.01). Matrix metalloprotease-2 (MMP-2), matrix metalloprotease-9 (MMP-9), VEGF and type-III collagen expressions in the wound tissue increased significantly (p < 0.05) when compared with the control burn wounds. Histological results showed an overall early recovery and regeneration in PGE-treated group when compared with control group. Conclusion: PGE possesses a significant wound-healing activity in full-thickness burn wounds in rats. Therefore, it can potentially be developed for the management of burns.

scholarly journals The Effect of Educated Platelets on the Healing Process of Burn Wounds in Rats: Educated Platelets in Rat Burn Wound Healing

2021 ◽  
Vol 8 ◽  
Author(s):  
Orkun Ilgen ◽  
Sefa Kurt ◽  
Osman Yilmaz ◽  
Murat Celiloglu
Keyword(s):  
Wound Healing ◽  
Burn Injury ◽  
Healing Process ◽  
Recovery Period ◽  
Control Group ◽  
Study Group ◽  
Second Line ◽  
Burn Wound ◽  
Wound Area ◽  
Burn Wound Healing

Burns are serious life-threatening health problems. This study was performed to determine the effect of educated platelets on burn wound healing process. 28 female wistar albino 200-220 gr rats were randomly divided into four groups. Group A1 rats(n:7) were the first-line burnt group from which blood samples are extracted to develop platelet-rich plasma(PRP) with the educated platelets that have a response to burn injury. Group B1 rats(n:7) were the unburnt group with ordinary platelets. Group A2 rats(n:8) were the second-line burnt group which was given PRP with educated platelets. Group B2 rats(n:6), as control group, were the second-line burnt group which was given PRP with ordinary platelets. Photos of rats' dorsum were taken by digital camera on the first day and 21st day of the study. Wound healing was determined by scar surface area. In the study group (Group A2) mean wound area was  53±37 mm², in the control group (Group B2) mean wound area was 114±55 mm² on the last day of the experiment. The sizes of the wounded areas were significantly lower in the study group compared with the control group (p: 0.039). Educated platelets seem to facilitate the recovery period of burn wound healing in rats.

scholarly journals Effects of Glutathione on Growth, Intestinal Antioxidant Capacity, Histology, Gene Expression, and Microbiota of Juvenile Triploid Oncorhynchus mykiss

2021 ◽  
Vol 12 ◽  
Author(s):  
Chang’an Wang ◽  
Baohui Su ◽  
Shaoxia Lu ◽  
Shicheng Han ◽  
Haibo Jiang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Oncorhynchus Mykiss ◽  
Antioxidant Capacity ◽  
Control Diet ◽  
Alpha Diversity ◽  
Control Group ◽  
Feed Conversion ◽  
Tnf Α ◽  
Significant Difference ◽  
Feed Conversion Rate

This study aimed to demonstrate the effects of dietary glutathione (GSH) on growth, intestinal antioxidant capacity, histology, gene expression, and microbiota in juvenile triploid rainbow trout (Oncorhynchus mykiss). Different diets (G0-control, G100, G200, G400, and G800) containing graded levels of GSH (0, 100, 200, 400, and 800mgkg−1) were fed to triplicate groups of 30 fish (initial mean weight 4.12±0.04g) for 56days. G400 had significantly improved weight gain and feed conversion rate. Based on the broken-line regression analysis, the optimum dietary GSH level was 447.06mgkg−1. Catalase and superoxide dismutase activities were significantly higher in G200–G800. G200 had significantly lower malondialdehyde content. The height of the intestinal muscular layer in G400 was significantly higher than that of the control group. Intestinal PepT1 and SLC1A5 gene expression was significantly increased, and the highest was observed in G400. TNF-α, IL-1β, IL-2, and IL-8 expression were significantly decreased than that of G0. Next-generation sequencing of the 16S rDNA showed a significant difference in alpha diversity whereas no differences in beta diversity. On the genus level, LefSe analysis of indicator OTUs showed Ilumatobacter, Peptoniphilus, Limnobacter, Mizugakiibacter, Chelatococcus, Stella, Filimonas, and Streptosporangium were associated with the treatment diet, whereas Arcobacter, Ferrovibrio, Buchnera, Chitinophaga, Stenotrophobacter, Solimonadaceae, Polycyclovorans, Rhodococcus, Ramlibacter, and Azohydromonas were associated with the control diet. In summary, feeding juvenile triploid O. mykiss 200–800mgkg−1 GSH improved growth and intestinal health.

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