scholarly journals Proximate Analysis, Antoxidant Property and Cytotoxicity Assessment for Pseuderanthemum Reticulatum Leaves

2021 ◽  
Vol 37 (4) ◽  
pp. 984-990
Author(s):  
S. Margrat Sheela ◽  
J. Rosaline Vimala
Keyword(s):  
Moisture Content ◽  
Paper Chromatography ◽  
Plant Material ◽  
Crude Protein ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Proximate Analysis ◽  
Bioactive Constituents ◽  
Plant Materials ◽  
Chemical Test

The bioactive constituents derived from plants attract the attention of researchers due to their potential applications in the medicinal field. In this regard, the proximate analysis and the cytotoxicity study of the plant materials play an important role in the phytochemical research. In the present work, estimation of total ash, moisture content, fiber content, crude protein, and carbohydrate were carried out under proximate analysis and the antioxidant activity of the anthocyanin present in the plant material was evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The separation of anthocyanin pigment from the plant material was done by paper chromatography (PC) technique and they are characterized by UV spectrum, chemical test and the Rf values obtained from paper chromatography. This study also investigated the in vitro cytotoxicity of Pseuderanthemum reticulatum leaves by means of MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)) assay PBMC (Peripheral Blood Mononuclear Cell). The results of the proximate analysis showed that the plant material contains 7.6% of moisture content, 16.6 % of total ash, 5.6% of crude protein, 23.0% of crude fiber, 3.82% of crude fat and 23.64 % of carbohydrate. The free radical scavenging ability of the separated anthocyanin was found to be 72.58% at 10 µg/mL. The cytotoxicity investigation showed that the aqueous extract possess the IC50 value of 161.5μg/mL. The High percentage of radical scavenging activity and low toxicity of the plant suggest that it can be extensively used for the investigation of the bioactive constituents and its applications.

scholarly journals Synthesis, Antiproliferative Activity and Radical Scavenging Ability of 5-O-Acyl Derivatives of Quercetin

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1608
Author(s):  
Stephen Lo ◽  
Euphemia Leung ◽  
Bruno Fedrizzi ◽  
David Barker
Keyword(s):  
Antiproliferative Activity ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Breast Cancer Cell Lines ◽  
Scavenging Activity ◽  
Plant Materials ◽  
Wide Range ◽  
Derivatives Of ◽  
Acyl Derivatives

Quercetin is a flavonoid that is found in many plant materials, including commonly eaten fruits and vegetables. The compound is well known for its wide range of biological activities. In this study, 5-O-acyl derivatives of quercetin were synthesised and assessed for their antiproliferative activity against the HCT116 colon cancer and MDA-MB-231 breast cancer cell lines; and their radical scavenging activity against the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical species. Four derivatives were found to have improved the antiproliferative activity compared to quercetin whilst retaining radical scavenging activity.

scholarly journals Determination of Nutritional Composition of Ackee’s Apple Blighia sapida and Monkey Kola Cola millenii

2020 ◽  
pp. 127-136
Author(s):  
Oyetade Joshua Akinropo ◽  
Bello Lukman Abidemi ◽  
Adesiyan Blessing Adedayo
Keyword(s):  
Vitamin C ◽  
Moisture Content ◽  
Crude Protein ◽  
Proximate Analysis ◽  
Nutritional Composition ◽  
The Other ◽  
Mineral Analysis ◽  
Crude Fibre ◽  
Fruit Samples

The proximate analysis of the fruit sample that homogenously sampled from the wild was taken and quartered to get appreciable weight fit for analysis. Due to it perishable nature the quartered sample for each of the fruit was stored in an air tight container and kept in the refrigerator at a temperature of about 4°C. For the determination of the nutritional composition, parameters which include their proximate, minerals, and vitamin C were quantitatively determined while the anti-nutrient composition were qualitatively and quantitatively analyzed. Compared to ackee’s apple, monkey cola was found to consist of 64.41% moisture content, 1.69% ash, 10.21% crude fibre, 1.25% crude fat, 4.44% crude fibre, 18.06% carbohydrates while ackee’s apple consist of 73.21% moisture, 1.49% ash, 9.38 % crude protein, 13.98% crude fat, 2.08% crude fibre, 0.86% carbohydrates and 4.45% of vitamin C. The mineral analysis for both samples was quantitatively determined using Atomic Absorption spectrophotometer (AAS). The minerals determined for ackee’s apple and monkey cola were magnesium which was 1391.65 ppm, calcium 628.23 ppm, sodium 506.96 ppm, potassium 3976.14 ppm, iron 1.0 ppm, copper 5.00, zinc 4.00, cobalt 3.0 and phosphorus 2616.90 ppm the iron however, was not detected. Ackee’s apple on the other hand, consist of magnesium 498.01 ppm, calcium 478.56 ppm, sodium 398.80 ppm, potassium 4970.18 ppm, copper 2.00, zinc 5.00, cobalt 3.0 and phosphorus 373.84 ppm. The qualitative screening of the anti-nutrients revealed the absence of phenol from both fruit samples while tannins were present only in the monkey cola. The flavonoids, phytic acid and oxalate were quantitatively determined to be 1240 mg, 625 mg and 155 mg for Monkey kola and 640 mg, 340 mg and 65 mg for Ackee’s apple.

scholarly journals Study of Antimicrobial, Antioxidant and Cytotoxicity Properties of Selected Plant Extracts for Food Preservative Applications

2021 ◽  
pp. 95-111
Author(s):  
Tania Islam ◽  
Md Nazrul Islam ◽  
Wahidu Zzaman ◽  
Md Morsaline Billah
Keyword(s):  
Pathogenic Bacteria ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antimicrobial Properties ◽  
In Vivo Studies ◽  
Diffusion Method ◽  
Plant Materials ◽  
Food Preservative

An attempt has been made to evaluate antimicrobial, antioxidant and cytotoxicity properties of extracts from onion (Allium cepa L.), garlic (Allium sativum), leaves of guava (Psidium guajava), papaya (Carica papaya), tea (Camellia sinensis), baen (Avicennia alba) and keora (Sonneratia apetala), respectively to apply as natural preservatives for tomatoes. The air-dried plant materials of the respective plant species were subjected to ethanol-methanol extraction, concentrated and stored at 4 °C before use. The extracts were dissolved in 95% ethanol for analysis of antioxidant and antimicrobial properties. Of the extracts tested, tea extracts showed the highest zone of inhibition against several pathogenic bacteria (E. coli 35.0±3.2 mm; P. aeruginosa 29.3±2.6 mm; S. typhi 28.4±2.1 mm and S. pyogenes 27.7±3.7 mm) using the disc diffusion method. In regard to DPPH free radical scavenging assay, keora and guava extracts showed the highest percentage of radical scavenging activity with the values of 89.64± 0.18 and 89.39± 0.88, respectively, which were in agreement with higher total antioxidant capacity (TAC) of these extracts obtained by the phosphomolybdenum method. Brine shrimp lethality bioassay for cytotoxicity assessment showed LC50 of 132.54 ± 18.99 µg/mL for the leaf extract of keora which was found to be most toxic among all studied extracts. The initial results indicated that the extracts could be used for food preservative applications based on the antimicrobial, antioxidant and cytotoxicity properties of the tested extracts. However, efficacy, stability and safety issues need to be addressed with both in vitro and in vivo studies.

scholarly journals Properties of Ginkgo biloba L.: Antioxidant Characterization, Antimicrobial Activities, and Genomic MicroRNA Based Marker Fingerprints

2020 ◽  
Vol 21 (9) ◽  
pp. 3087 ◽  
Author(s):  
Katarína Ražná ◽  
Zuzanna Sawinska ◽  
Eva Ivanišová ◽  
Nenad Vukovic ◽  
Margarita Terentjeva ◽  
...  
Keyword(s):  
Ginkgo Biloba ◽  
Radical Scavenging Activity ◽  
Bacterial Species ◽  
Radical Scavenging ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Bioactive Constituents ◽  
Leaf Extracts ◽  
Minimal Inhibition Concentration ◽  
Fresh Matter ◽  
Antioxidant Power

The aim of this study was to characterize extracts from the leaves of Ginkgo biloba L. from selected Slovakian localities in terms of the content of bioactive constituents, antioxidants and their antimicrobial properties. The results indicated that the content of antioxidants was sample-specific, and this specificity was statistically significant. Ginkgo biloba L. from the locality of Košice had the best activity determined by the free radical scavenging activity (DPPH) (1.545 mg Trolox equivalent antioxidant capacity (TEAC)/g fresh matter (FM)) as well as the molybdenum-reducing antioxidant power (35.485 mg TEAC/g FM) methods. The highest content of total polyphenols (2.803 mg gallic acid equivalent (GAE)/g FM) and flavonoids (4.649 μg quercetin equivalent (QE)/g FM) was also detected in this sample. All samples of G. biloba leaf extracts showed significant antimicrobial activity against one or more of the examined bacterial species, and Staphylococcus aureus subsp. aureus CCM 2461 was found to be the most susceptible (minimal inhibition concentration MIC50 and MIC90 values of 64.2 and 72.2 µg/mL, respectively). Based on the results it was concluded that Ginkgo biloba L. extracts can be used as antimicrobial and antioxidant additives. Selected miRNA-based molecular markers were used to examine the environmental adaptability of Ginkgo biloba L. An almost-complete genotype clustering pattern based on locality was determined in the analysis that involved a species-specific gb-miR5261 marker. Morphologically specific exemplar, cv. Ohatsuki, was excluded.

Radical scavenging activity ofCrocus sativus L. extract and its bioactive constituents

2005 ◽  
Vol 19 (11) ◽  
pp. 997-1000 ◽  
Author(s):  
A. N. Assimopoulou ◽  
Z. Sinakos ◽  
V. P. Papageorgiou
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Bioactive Constituents

scholarly journals In vitro antioxidant, antiglycation, and MGO scavenging effects of Leonurus sibiricus water extract

2021 ◽  
pp. 84-95
Author(s):  
Xiu Qing Chong ◽  
Kirtani AP Anamalay ◽  
Okechukwu Patrick Nwabueze ◽  
Hor Kuan Chan
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Water Extract ◽  
Antioxidant Property ◽  
Future Research ◽  
Total Phenolic ◽  
Bioactive Constituents ◽  
Bioactive Component ◽  
Glycation End Products

Recently, extensive research has been conducted to evaluate the inhibitory activity of different plant species on the advanced glycation end products (AGEs). L. sibiricus is a traditional herb that has been used for postpartum confinement meals in Sarawak, Malaysia and pharmacologically possess anti-hemorrhagic, antioxidant, anti-diabetic, and anti-cancer. The aim of this research was to evaluate the antioxidant, anti-AGEs, and preliminary biochemical screening of bioactive component present in L. sibiricus in water extract. Free radical scavenging activity of L. sibiricus was evaluated via DPPH, hydroxyl radical, nitric oxide, lipid peroxidation, chelating capacity, and total phenolic content was evaluated comparing with gallic acid. Inhibition of formation AGEs by L. sibiricus was evaluated using BSA-MGO, BSA-glucose and MGO scavenging assays. Flavonoids, phenols, saponins, alkaloids, phytosterol, and diterpenoids were identified in L. sibiricus extract. It also seems to inhibit early and late formation of AGE and MGO scavenging ability. L. sibiricus was able to inhibit the formation of early and late formation of AGE through the scavenging of the formation of reactive dicarbonyl intermediates and reduce the formation of methylquinoxaline adducts through the scavenge of MGO. The inhibition of AGEs formation by L. sibiricus maybe due its antioxidant property and the presence phytochemical bioactive constituents which has been previously reported to possess antioxidant and anti-AGE activity. Future research is ongoing to identify the adducts formed because of MGO scavenging by L. sibiricus using HPLC.

scholarly journals Pharmacological investigations of Litsea lancifolia (Roxb.) Hook. F.

2020 ◽  
Vol 49 (1) ◽  
pp. 179-183
Author(s):  
Israt Jahan Bulbul ◽  
Mohammad Rashedul Haque ◽  
Mohammad Abdur Rashid
Keyword(s):  
Ethyl Acetate ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Bioactive Constituents ◽  
Cns Depressant ◽  
Acid Potential ◽  
Cns Depressant Activity ◽  
Soluble Fractions

The petroleum ether, chloroform and ethyl acetate soluble fractions of methanolic extract of Litsea lancifolia Roxb. leaves were subjected to different pharmacological screenings to explore its potential as anti-oxidant, antimicrobial, peripheral analgesic, hypoglycemic and CNS depressing agent. The ethyl acetate soluble fraction showed highest total phenolic content and free radical scavenging activity compared to the standard, acetyl salicylic acid. Potential antimicrobial activity was shown against P. aeruginosa (23.50 mm), E. coli (22.33 mm), B. cereus (18 mm) and S. paratyphi (18 mm). The crude extract demonstrated significant peripheral analgesic (p < 0.01) activity with 69.45 and 77.96% inhibition of acetic acid-induced writhing at 100 and 200 mg/kg b.w., respectively. The crude methanolic extract also showed significant hypoglycemic activity (p < 0.01) at a dose of 500 mg/kg/day on the 7th day of treatment. All the organic soluble fractions exhibited noteworthy (p < 0.001) CNS depressant activity. Taken together, the plant can be considered as a good material for further chemical investigation to isolate the bioactive constituents.

scholarly journals EFFECT OF STORAGE MATERIALS ON THE PROXIMATE COMPOSITION OF TOMATO (Lycopersicon Esculentum) POWDER

2020 ◽  
Vol 4 (2) ◽  
pp. 203-206
Author(s):  
RUKAYAT OLADIPUPO ◽  
K. A. Yusuf ◽  
G. Salawu
Keyword(s):  
Vitamin C ◽  
Moisture Content ◽  
Crude Protein ◽  
Storage Period ◽  
Proximate Analysis ◽  
Aluminium Foil ◽  
Crude Fibre ◽  
Storage Materials ◽  
Tomato Powder

The effect of storage materials on the quality of tomato powder was studied. Fresh, firm and matured red tomatoes were washed, sorted and sliced to a uniform thickness of 5mm. The sliced tomatoes were pre-treated by dipping in 1% calcium chloride (CaCl2) and 0.2% potassium metabislphite (KMS) solution for 10 minutes at room temperature. 200g each of the sample was dried at temperatures of 600C until equilibrium moisture content was attained. The dried tomatoes were ground into powder and stored in Black polyethylene [BPE], White polyethylene [WPE], and Laminated Aluminium Foil [LAF] pouches for 6 weeks. The moisture content, crude protein, crude fibre, fat, total ash, and vitamin C of the tomato powder were determined using the method prescribed by AOAC. The carotene contents were determined by HPLC. The proximate analysis was repeated after 2 weeks, 4 weeks and 6weeks of storage.  There was an increase in moisture content and ash content after 6 weeks of storage while a decrease in value of protein, fat and vitamin C content occurred after the storage period. All the parameters measured were best retained by the laminated aluminium foil (LAF) compared to black polyethylene (BPE) and white polyethylene (WPE) packaging material for the entire period of the study.

scholarly journals Phenolic Content and DPPH Radical Scavenging Activity of the Flowers and Leaves of Trifolium repens

2013 ◽  
Vol 8 (1) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Agnieszka Kicel ◽  
Maria Wolbiś
Keyword(s):  
Trifolium Repens ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
In Vitro Test ◽  
Light Spectrum ◽  
Leaf Extracts ◽  
Plant Materials

In order to facilitate the quality control of Trifolium repens flowers and leaves, a RP-HPLC method with UV detection was developed for the simultaneous quantitative determination of flavonols and isoflavones. The total flavonoid and phenolic (TPC) contents were determined spectrophotometrically in a visible part of the light spectrum at 425 and 760 nm, respectively. Additionally, evaluation of the antioxidant properties of the plant materials was performed using the DPPH in vitro test. The results showed that the flowers are the richest source of phenolics ranging from 28.7 to 38.8 mg GAE/g, and flavonoids, calculated for hyperoside, up to 20 mg HP/g, which hydrolyzed mainly to flavonols (the quercetin level greater than 6 mg/g). T. repens is poor in isoflavones; similar quantities of ca. 0.2 mg/g were detected in the flowers and leaves. The flower and leaf extracts showed antioxidant activity towards DPPH with EC50 values ranging from 72.3 to 179.3 μg/mL. Significant linear correlations were found between antioxidant potentials of the studied plant materials and total phenolic and flavonoid contents determined by HPLC and spectrophotometric methods ( R2 in the range of 0.97 - 0.99).

Scanning Electron Microscopy analysis, Fourier Transform Infrared Spectrum Structure Profile characterisation, and in vitro biological effect of Salvia officinalis L. Phytocompounds

2020 ◽  
Vol 21 ◽  
Author(s):  
Issam Saidi ◽  
Fatma Guesmi ◽  
Ramzi Amari ◽  
Sana Ncib ◽  
Najla Hfaiedh
Keyword(s):  
Fourier Transform ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Fourier Transform Infrared ◽  
Salvia Officinalis ◽  
Bioactive Constituents ◽  
Antioxidant Power ◽  
Scanning Electron

Background: The aims of this research is to investigate the potential activity of Salvia officinalis and various hairs responsible of secretion of essential oil. In Tunisia, biological activity of Salvia officinalis is poorly recorded. Salvia leaves contain various types of hairs (glandular and non-glandular). Methods: The investigation of different trichomes were carried out by scanning electron microscope (SEM) apparatus. Antiradical potential were assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assay. Antimicrobial activity was analysed using disc diffusion assay. The extracts of Salvia officinalis (SvOf), showed the following order of richness in phenolic contents: methanolic (70.76 mg GAE/g DW), aqueous (43.76 mg GAE/g DW) and infusion (9.42 mg GAE/g DW). The methanolic fraction records the highest levels of flavonoids (77 mg QE/g DW) compared with the aqueous extracts (33.19 mg EQ/g DW) and infusion (26.25 mg EQ/g DW). ME-SvOf showed higher value of free radical scavenging activity towards DPPH free radical and ferric reducing power. Results: The results revealed that main bioactive constituents in the methanolic fractions of sage leaves generally exhibited higher antibacterial effects. Overall, sage phytocompounds constitute a promising approach for the treatment of infectious diseases. Discussion: Functional groups detected in S. officinalis by Fourier transform infrared spectrophotometer (FT-IR) were mainly phenols, saccharides, amine and aromatic (Ar-H). Conclusion: Antiradical and antibacterial activities of Salvia officinalis are mainly due to phenolic content and other bioactive compounds. Non-glandular hairs are the most important trichomes in the Salvia leaves.

Sign in / Sign up

Export Citation Format

Share Document