Process Development for Antioxidant Extraction from Wet Pomegranate Peel

2021 ◽  
Vol 64 (1) ◽  
pp. 191-202
Author(s):  
Xingzhu Wu ◽  
Chandrasekar Venkitasamy ◽  
Tara McHugh ◽  
Zhongli Pan
Keyword(s):  
Antioxidant Activity ◽  
Animal Feed ◽  
Water Soluble ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
List Type ◽  
Pomegranate Peel ◽  
Important Indicator ◽  
Extract Yield ◽  
Water As Solvent

HighlightsA novel process for antioxidant extraction from wet pomegranate peel (WPP) was developed using water as solvent.Effects of extraction parameters on extract yield, quality, composition, and color were quantified.Extraction from WPP achieved 10% higher extract yield compared with dried peel.The WPP extraction process is more energy-efficient than extraction from dried peel.Abstract. Pomegranate peel (PP) is underused as animal feed or discarded in landfills, causing environmental pollution, even though it has a unique polyphenol profile with health-promoting properties. Past research has focused on the potential utilization of dried pomegranate peel (DPP), involving extended processing time, energy usage, and potential quality loss. The objective of this study was to develop a process to extract water-soluble antioxidants from wet pomegranate peel (WPP) and compare its antioxidant attributes with that of DPP. The WPP was sliced and ground into two sizes, and phenolics were extracted in water at different temperatures (20°C, 30°C, 40°C, 50°C, and 60°C), solvent (water) ratios (1:1, 2:1, 4:1, 6:1, and 8:1), and extraction times (2, 3, 4, 5, and 6 min). WPP extraction was compared with DPP extraction using hot air (HA, 40°C) and infrared radiation (IR, 60°C). The total phenolic yield (TPY) from WPP ranged from 10.37% to 12.80%, and the extraction rate increased significantly with increasing extraction time, temperature, and solvent ratio. DPPH scavenging activity (DSA), an important indicator of antioxidant activity, was in the range of 5.366 to 6.591 g g-1 and decreased only with the increase in temperature. Extraction using WPP particles resulted in 10% more extract yield and 2.5% more TPY compared to DPP powders with similar DSA. This study demonstrated that WPP could be used for the extraction of phenolic compounds with high antioxidant activity and without energy-intensive drying. Keywords: . Infrared drying, Peel composition, Polyphenol, Principal component analysis, Wet pomegranate peel.

scholarly journals Effects of Extraction Methods and Conditions on Bioactive Compounds Extracted from Phaeodactylum tricornutum

2020 ◽  
Vol 67 (4) ◽  
pp. 1250-1261
Author(s):  
Saniye Akyıl ◽  
Işıl İlter ◽  
Mehmet Koç ◽  
Zeliha Demirel ◽  
Ayşegül Erdoğan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Extraction Method ◽  
Phaeodactylum Tricornutum ◽  
Phenolic Content ◽  
Extraction Methods ◽  
Extraction Time ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Microwave Extraction

The effect of homogenization, ultrasound and microwave extraction methods and conditions on fucoxanthin content, total phenolic content and antioxidant activity of extracts obtained from Phaeodactylum tricornutum were investigated in this study. The solvent/biomass ratio was the most effective parameter on fucoxanthin content, total phenolic content and antioxidant activity. The maximum fucoxanthin content (5.60 ± 0.06 mg/g) and antioxidant activity (763.00 ± 15.88 EC50 μg/mL extract) were obtained with the homogenization extraction method whose optimum conditions were 1.93% biomass/solvent ratio, ~5200 rpm homogenization rate and 14.2 min extraction time. Although the ultrasonic extraction method has reached the approximately same level of fucoxanthin content (5.24 ± 0.07 mg/g)), TPC (67.68 ± 1.58 mg gallic acid/L) and antioxidant activity (619.90 ± 17.16 EC50 μg/mL extract) at an amplitude of 55.72%, a higher biomass/solvent ratio (2.72%) and a longer extraction time (17.37 min) have been required. The lowest fucoxanthin content, total phenolic content and antioxidant activity were determined for the microwave extraction method.

scholarly journals Culinary Method Affects the Antioxidant Activity of Collard Greens (Brassica oleracea)

2012 ◽  
Vol 1 (4) ◽  
pp. 66
Author(s):  
Alexander Clifford ◽  
Paul Dawson
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Treated Group ◽  
Water Soluble ◽  
Total Phenolic ◽  
Thermal Treatments ◽  
Treatment Groups ◽  
Collard Greens ◽  
Cooking Water ◽  
Group 2

<p>The antioxidant activity of collard greens was determined after exposed to eight different thermal treatments: 1) untreated raw group, 2) short simmer 3short simmer water 4) short simmer + saute’, 5) saute’ 6) long simmer 7) long simmer water 8) long simmer + saute’. After treatment, total phenolic content (TPC) expressed in gallic acid equivalents/sample concentration (GAE/conc.), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferrous ion chelating (FIC) antioxidant assays were determined. The sauté treated group showed the highest TPC (8.2858 GAE/conc.) followed by the raw group (8.0361) and the short simmer + sauté group (7.6227). The raw group showed the highest DPPH activity (7.7952% inhibition/conc.) followed by the sauté group (7.5877) and the short simmer + sauté group (7.4753). In both of these assays the addition of a sauté treatment to either short or long simmered treatment increased the antioxidant activity of samples compared to just the short or long simmer treatment alone. Additionally both TPC and DPPH assays showed greater antioxidant activity in the cooking water reserved from a long simmer treatment compared to the reserved cooking water of a short simmer treatment suggesting significant (p&lt;0.05) leeching of antioxidants from collard greens into the water related to the duration of aquathermal treatment. Similar trends were not found in the results of the FIC chelating assay where both long and short simmer treatment groups showed the highest chelating abilities and the reserved cooking water from both treatments showed the lowest chelating abilities. This suggests that chelators contained in collard greens were not relatively water soluble and therefore not negatively affected byaquathermal treatments.</p>

In Vitro Antimicrobial and Antioxidant Activity of Commercial Rosemary Extract Formulations

2009 ◽  
Vol 72 (8) ◽  
pp. 1744-1752 ◽  
Author(s):  
ANJA KLANČNIK ◽  
BERNARDA GUZEJ ◽  
MAJDA HADOLIN KOLAR ◽  
HELENA ABRAMOVIČ ◽  
SONJA SMOLE MOŽINA
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Water Soluble ◽  
Total Phenolic ◽  
Gram Negative ◽  
Antimicrobial And Antioxidant Activity

Phenolic plant extracts are sources of natural bioactive compounds, which can inhibit the rate of food spoilage. MIC and MBC concentrations of four oil- or water-soluble rosemary (Rosmarinus officinalis) extracts against gram-positive (Bacillus and Staphylococcus) and gram-negative (Campylobacter and Salmonella) bacteria were determined by using disk diffusion, agar dilution, and broth microdilution methods, as well as bacterial survival kinetics in a macrodilution test. To describe the antioxidant properties of the extracts, the reducing power, free radical scavenging effectiveness, and β-carotene bleaching test were used. The antimicrobial and antioxidant activity depended on the concentration and chemical nature of the phenolic compounds in the extracts. Gram-positive bacteria were more sensitive than were gram-negative bacteria, especially for oil-soluble extracts with carnosic acid as the major phenolic compound. A microdilution method based on ATP measurement was found to be a useful, rapid technique for determining antibacterial efficiency, and its results correlated well with MICs from survival curve measurement. Reducing power and free radical scavenging effectiveness was higher in water-soluble formulations, according to their higher total phenolic content, but in an aqueous emulsion system of linoleic acid, they exhibited lower antioxidant activity. This correlated well with the higher efficiency of antimicrobial activity of oil-soluble formulations, despite the lower total phenolic content of these extracts.

scholarly journals Heterosis and antioxidant compounds of sweet corn breeding lines and their F1 hybrid

Genetika ◽  
2014 ◽  
Vol 46 (3) ◽  
pp. 807-813 ◽  
Author(s):  
Boris Kraptchev ◽  
Roumiana Vassilevska-Ivanova ◽  
Lydia Shtereva
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Sweet Corn ◽  
Natural Antioxidants ◽  
Kernel Weight ◽  
Water Soluble ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
F1 Hybrid ◽  
Antioxidants Activity

In this research, the mid-parent heterosis and the levels of phenolic, flavonoids compounds and antioxidants activity among two selected sweet corn parental lines and their F1 hybrid Zaharina were evaluated. Substantial positive mid-parent heterosis (MPH) was found for parent-hybrid triplet for ear weight and for plant height. Among all other traits, insertion height had the greatest heterosis and ear diameter had the lowest. Negative mid-parent heterosis exhibited only the trait 1000-kernel weight. Data analysis indicated significant differences in the contents of total phenolic content, total flavonoids content, water-soluble antioxidant capacity, lipid-soluble antioxidant capacity, and antioxidant activity among parent-hybrid triplet. The results suggest that F1 hybrid Zaharina can be considered as good source of natural antioxidants since it extracts were found to possess high antioxidant activity.

scholarly journals PENGARUH JENIS PELARUT TERHADAP EKSTRAKSI DARI KULIT BUAH NAGA MERAH (Hylocereus polyrhizus)

KOVALEN ◽  
2019 ◽  
Vol 5 (3) ◽  
pp. 271-279
Author(s):  
Amalia Noviyanty ◽  
Chitra Anggriani Salingkat ◽  
Syamsiar Syamsiar
Keyword(s):  
Total Phenolics ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Fruit Peel ◽  
Extract Yield ◽  
Randomized Design ◽  
Acetone Water ◽  
Dragon Fruit ◽  
Ic50 Value ◽  
Ic50 Values

This study aims to determine the effect of the type of solvent on extract yield, total phenolics and IC50 values of the skin of red dragon fruit and also obtain the best type of solvent that provides extract rendement, total phenolics and the highest or best IC50 value for extracting red dragon fruit peels. There are 3 types of solvents used (95% ethanol, ethyl acetate and acetone: water (7: 3) with a sample-solvent ratio (5:1 v/b).  The data obtained were analyzed using a Completely Randomized Design that was applied to observations of extract rendemen, phenolics total and IC50 values, if the treatment had a very significant or significant effect followed by continued BNJ test at 1% or 5% level. The results showed that the solvent ratio very significantly affected the extract rendemen, phenolics total and IC50 value of red dragon fruit peel extract.  Ethanol 95% solvent is the best solvent for extracting dragon fruit peels because it has the highest yield (26.15%), total phenolic (64.75 ppm) and antioxidant activity (IC50 value) (120.53 ppm) highest or best. Keywords : IC50, the red dragon fruit skin, solvent ratio, extract yield, phenolics total

scholarly journals KOMPOSISI SENYAWA FENOL DAN POTENSI ANTIOKSIDAN DARI KACANG-KACANGAN: REVIEW

2020 ◽  
Vol 14 (01) ◽  
pp. 91
Author(s):  
Nurud Diniyah ◽  
Sang-Han Lee
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Seed Coat ◽  
Water Soluble ◽  
Grain Legume ◽  
Total Phenolic ◽  
Hydroxyl Groups ◽  
Chemical Structures ◽  
Legume Seeds

Legumes are good dietary source of bioactive phenolic compounds which play significant roles in many physiological as well as metabolic processes. Flavonoids, phenolic acids and condensed tannins are the main phenolic compounds that are provide in legume seeds. Majority of the phenolic compounds are serving in the legume seed coats. The majority of seed coat of legume seeds are phenolic acids and flavonoids (mainly catechins and procyanidins). Gallic and protocatechuic acids are common in kidney bean and mung bean. Almost 70% of total phenolic compounds in lentils and cranberry beans (seed coat) are catechins and procyanidins. The antioxidant activity of phenolic compounds is in lineal intercourse with their chemical structures such as number as well as position of the hydroxyl groups. Processing mostly conducts to the alleviation of phenolic compounds in legumes because of chemical rearrangements. Phenolic content also lessen due to leaching of water-soluble phenolic compounds into the cooking water. This review provides comprehensive information of phenolic compounds identified in grain legume seeds along with discussing their antioxidant. Keywords: antioxidant activity, legume seeds, phenolic compounds, processing

scholarly journals THE COMPARATIVE STUDY OF ANTIOXIDANT PROPERTIES OF SOME SAMBUCUS L. SPECIES

2019 ◽  
pp. 127-137 ◽  
Author(s):  
Любовь (Lyubov') Николаевна (Nikolaevna) Скрыпник (Skrypnik) ◽  
Алина (Alina) Андреевна (Andreevna) Курашова (Kurashova)
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Properties ◽  
Total Content ◽  
Water Soluble ◽  
Biologically Active ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Sambucus Ebulus ◽  
Amperometric Method

The antioxidant properties of fruits, flowers, leaves, bark (or stem) of elderberry (Sambucus nigra L.), red elderberry (Sambucus racemosa L.) and dwarf elder (Sambucus ebulus L.) were investigated. The total content of anthocyanins and total phenolic compounds content by using of Folin-Ciocalteu assay were determined spectrophotometrically. The total content of water-soluble antioxidants was investigated by amperometric method. The antioxidant activity (AOA) of plant extracts was measured using DPPH (1,1-diphenyl-2-picrylhydrazyl) radical, ABTS (2,2’azinobis(3)ethylbenzthiazoline-6- sulfonic acid) radical and FRAP (ferric reducing antioxidant power) assays. It was established that the fruits of plants of the genus Sambucus L. were characterized by the maximum level of all studied antioxidants. High content of anthocyanins and phenolic compounds was determined in the leaves of elderberry and dwarf elder. The flowers of these elderberry species were distinguished by a high total content of water-soluble antioxidants. The highest antioxidant activity was observed in fruits extracts in comparison with other parts of the plant. Higher antioxidant activity was identified in the extract of the fruits of elderberry and dwarf elder than of the red elderberry fruits. The most optimal method for evaluating the antioxidant activity of elder extracts was the FRAP assay, which showed the highest correlation between AOA and individual antioxidant components, compared to DPPH and ABTS assays. Comparative analysis of antioxidant content and antioxidant activity of various plant parts of three elderberry species showed that the most promising sources of biologically active substances with antioxidant properties are fruits and flowers of elderberry and dwarf elder.

scholarly journals Antioxidant and antibacterial insights into the leaves, leaf tea and medicinal roots from Astragalus membranaceus (Fisch.) Bge.

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Anim Okyere Samuel ◽  
Bao-Ting Huang ◽  
Yuan Chen ◽  
Feng-Xia Guo ◽  
Dou-Dou Yang ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Antimicrobial Activities ◽  
Water Soluble ◽  
Astragalus Membranaceus ◽  
Total Amino Acid ◽  
Total Phenolic ◽  
Plant Leaves ◽  
Bioactive Components ◽  
Wide Range

AbstractUsed as traditional Chinese medicine, Astragalus membranaceus (Fisch.) Bge. (A. membranaceus) roots are also used as tonic food material in a wide range of applications, while the leaves are left in the field, unused. Therefore, comprehensively exploring and utilizing the leaves will inevitably reduce the associated resource waste and environment pollution. In this study, the plant leaves were processed into tea using green tea processing technology. Bioactive components, antioxidant and antibacterial activities of the Leaf Tea (LT) and Dry Leaves (DL) were studied, and compared to that of the Dry Roots (DR). The results showed that the polysaccharides content (POL) in the DR (20.44%) was twice as high as the DL (10.18%) and LT (8.68%). However, the DL contained 36.85% more water-soluble extracts (WSE), 35.09% more ethanol-soluble extracts (ESE), 409.63% more total flavonoid content (TFC), 221.01% more total phenolic content (TPC) and 94.34% more proteins, and the LT contained 26.21% more WSE, 40.64% more ESE, 326.93% more TFC, 191.90% more TPC and 37.71% more proteins. The total amino acid (AA) content in the DR was 8.89%, while in that of the DL and LT were 24.18% and 28.96% respectively, nearly 3-times higher than that of the DR. The antioxidant activity of DR was much lower than those of DL and LT, both of which had antioxidant activity closer to that of Vitamin C (VC) and the antioxidant activities were even stronger when the optimal concentration was reached. Except for Aspergillus niger and Staphylococcus aureus, the DL and DR exhibited inhibition activities to Salmonella, Bacillus subtilis, Escherichia coli and yeast, while the LT had antimicrobial activities against all the strains except for A. niger. In summary, compared with the most commonly used DR, the DL and LT from A. membranaceus contained higher bioactive components, and stronger antioxidant and antimicrobial activities. Producing leaf tea may be an appropriate way to economically and reasonably utilize the plant leaves which are by-products.

scholarly journals Evaluation of bio-detoxification of Jatropha curcas seed cake and cottonseed cake by basidiomycetes: nutritional and antioxidant effects

2021 ◽  
Author(s):  
Marina Borges Guimarães ◽  
Félix Gonçalves de Siqueira ◽  
Raquel Bombarda Campanha ◽  
José Antônio de Aquino Ribeiro ◽  
Pérola Oliveira Magalhães Dias Batista ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Animal Feed ◽  
Total Phenolic ◽  
Cottonseed Cake ◽  
Total Phenolic Compounds ◽  
Seed Cake ◽  
Increase Protein Content ◽  
Jatropha Seed ◽  
Antioxidant Effects

Abstract Poultry and swine are the major proportion of the livestock industry in terms of output value. To meet the growing need for protein sources in these sectors, the use of biomasses coming from agro-industrial residues can be an interesting option in the coming years. This study aimed to evaluate the capacity of seven basidiomycetes to grow, detoxicate, increase protein content, and also its antioxidant activity when grew in pure Jatropha seed cake (JSC) and cottonseed cake (CSC) biomasses and mixtures containing 50% of lignocellulosic biomasses from coconut husks and Acrocomia aculeata (macauba cake). Results showed that five basidiomycetes were able to grow in these substrates. F. hepatica, P. lecomtei, and P. pulmonarius presented the highest bio-detoxification capacity. All treatments showed a reduction in total phenolic compounds (TPC) and antioxidant activity, but treatments with coconut husks showed lower reductions. Results also indicated that there are molecules produced by basidiomycetes responsible for antioxidant activity other than phenolic compounds. These results indicated that basidiomycetes could detoxify JCS and CSC biomasses, suggesting their possible use in animal feed and that the addition of coconut husks in JSC and macauba cake in cottonseed cake can promote greater colonization by fungi.

scholarly journals OPTIMIZATION OF MICROWAVE- AND ULTRASONIC-ASSISTED EXTRACTION OF MAHKOTA DEWA (PHALERIA MACROCARPA [SCHEFF.] BOERL.) FRUIT PULP

2020 ◽  
pp. 32-37
Author(s):  
ROSITA HANDAYANI ◽  
ANDRE BANGUN ◽  
PASKAH DEWI DEBORAH ◽  
ABDUL MUN’IM
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Choline Chloride ◽  
Deep Eutectic Solvent ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Water Addition ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

Objective: This work aimed to determine the optimal extraction conditions of Mahkota dewa using different extraction methods: microwave-assistedextraction with ethanol (MAE-ethanol), ultrasonic-assisted extraction using a choline chloride natural deep eutectic solvent (UAE-NADES), andSoxhlet and reflux extractions.Methods: Experimental design was performed through Box-Behnken. The efficiency of the methods was evaluated based on the total phenolic content(TPC) and antioxidant activity. The presence of relevant compounds was qualitatively assessed by high-performance liquid chromatography (HPLC).Results: For the MAE-ethanol method, optimum extraction was achieved with 65% ethanol, in 1 min under 30% microwave power, and a 1:12 sample–solvent ratio, which yielded a TPC and antioxidant activity of 62.79±0.74 mg gallic acid equivalents (GAE)/g powder and 30.48±0.32%, respectively. InUAE-NADES, the optimal conditions were choline chloride: lactic acid ratio of 1:4 with a 50% water addition, 20 min extraction (TPC and antioxidantactivity of 65.25 mg GAE/g powder and 26.45±0.02%). The Soxhlet and reflux methods gave a TPC of 49.68±0.59 and 50.98±0.54 mg GAE/g powder,and antioxidant activity of 22.87±0.13% and 25.97±0.34%, respectively. Qualitative HPLC analysis showed the presence of quercetin, mahkoside A,4,6-dihydroxy-4-methoxybenzophenone-2-O-β-D-glucopyranoside, and mangiferin.Conclusion: MAE-ethanol was more efficient than the conventional Soxhlet and reflux methods and performed similarly to UAE-NADES in terms of TPC.

Sign in / Sign up

Export Citation Format

Share Document