scholarly journals Consortium of endophytic bacteria and rhizobacteria effectively suppresses the population of Pratylenchus coffeae and promotes the growth of Robusta coffee

2020 ◽  
Vol 21 (10) ◽  
Author(s):  
Iis Nur Asyiah ◽  
Imam Mudakir ◽  
Mohammad Hoesain ◽  
Ankardiansyah Pandu Pradana ◽  
Achmad Djunaidy ◽  
...  

Abstract. Asyiah IN, Mudakir I, Hoesain M, Pradana AP, Djunaidy A, Sari RF. 2020. Consortium of endophytic bacteria and rhizobacteria effectively suppresses the population of Pratylenchus coffeae and promotes the growth of Robusta coffee. Biodiversitas 21: 4702-4708. Pratylenchus coffeae as the coffee-parasitic nematode can cause a yield loss from 28.7% to 78.4% on Robusta coffee (Coffea canephora A. Froehner). Environmentally friendly and sustainable efforts can be made to control this nematode by applying the antagonistic agents from the bacterial group. The application of antagonistic agents in a consortium has a better effectivity than a single application. This study was aimed to identify the effectivity of the consortium of endophytic bacteria and rhizobacteria formulated on a cost-effective molasse medium against the P. coffeae population and growth of Robusta coffee in a greenhouse. A total of nine bacterial isolates of genus Bacillus and Pseudomonas were used during experiment. The formulation was prepared by mixing the bacterial suspension and 2% molasses with a ratio of 1:1 (v/v). The greenhouse assay was performed following the completely randomized block design pattern with five treatments and five replications. Robusta coffee plant clone BP 936 was treated with a 10 mL (109 CFU mL-1) bacterial consortium mixture while control was treated with 10 mL sterilized water. Each plant was inoculated with P. coffeae 50 J2 after a week of bacterial consortium application. The result showed that all bacterial consortiums were effective in suppressing the population of P. coffeae in soil and root of Robusta coffee. All consortiums gave a better result than control in suppressing the population of P. coffeae. The best suppression of nematodes was shown by the Cst 02 consortium with the suppression value of 62.5% in the root and 76.1% in the coffee plant rhizosphere. The application of bacterial consortium increases the plant height (28.02-44.51%), number of leaves (127.27-168.18%), and leaf area (278.8-425.57%). In this study, suppression of P. coffeae population by bacterial consortium showed a positive correlation with plant growth. This study presents the information of endophytic bacteria and rhizobacteria consortium from Bacillus and Pseudomonas group that is effective in suppressing the population P. coffeae and improves the growth of Robusta coffee.

Author(s):  
Bistok Hasiholan Simanjuntak ◽  
Devi Kusuma Wardani

Robusta coffee plant propagation is recommended to be performed by vegetative methods, one of which is cuttings. Among plant organs that can be used for cuttings is the stem. The research objective was to determine which stem segment from cuttings was able to provide the best root and leaf growth. The layout study used a completely randomized block design (RBD) with an experimental treatment consisting of five stem segments, namely (1) the first stem segment from the upper shoot (A); (2) the second stem segment from the upper shoot (B); (3) the third stem segment from the upper shoot (C); (4) the fourth stem segment from the upper shoot (D); and (5) the fifth stem segment from the upper shoot (E); each treatment had five replications. Data analysis was performed statistically with analysis of variance (ANOVA) and Tukey's HSD (honestly significant difference) test with a significance level of P < 0.05. Robusta coffee cuttings stems from the second stem segment from the upper shoot were able to provide the optimal root growth (root length) and shoot growth (leaf sprout height and leaf sprout growth rate). Leaf sprout number derived from the cuttings was not affected by the stem segment.


Author(s):  
Novie Pranata Erdiansyah ◽  
Ade Wachjar ◽  
Eko Sulistyono ◽  
Supijatno Supijatno

Drought is one of the limiting factors for the growth and yield of coffee plants. Drought due to long dry season has caused many losses for coffee plantations. This study aimed to evaluate the response of four Robusta coffee clones to drought stress at seedling stage and determine the best clone with high tolerance to drought. The study was conducted in Indonesian Coffee and Cocoa Reseach Institute (ICCRI) Jember, East Java in November 2018–Mei 2019. The experimental design used a Randomized Block Design (RCBD) with two factors. The first factor has consisted of five levels drought through providing volume watering based coefficient and evaporation value of free water surface (Eo), namely: 0.5 Eo; 1.0 Eo; 1.5 Eo; 2.0 Eo; 2.5 Eo (control). The second factor has consisted of four Robusta coffee clones, namely: BP 409 (drought tolerant clone); BP 308; BP 939 and BP 358 (vulnerable to drought stres). The result showed that reduction in the volume of watering from 2.5 Eo until 0.5 Eo causes drought, reduce coffee growth linearly. Clone BP 409 and BP 939 had better tolerance of drought stress compared with BP 308 and BP 358. The anatomical adaptation of leaves of BP 409 to drought stress was by thickening of wax layer and palisade tissue. BP 939 thickened its leaves due to drought stress as a mechanism of adaptation to such condition.


BUANA SAINS ◽  
2018 ◽  
Vol 18 (1) ◽  
pp. 29
Author(s):  
Wiharyanti Nur Lailiya ◽  
Karuniawan Puji Wicaksono ◽  
Eko Widaryanto

Robusta coffee (Coffea canephora) is one of the commodities that have high economic value among other plantation crops. Coffee is also one of the three non-alcoholic beverages (coffee, tea, chocolate) are widespread. Indonesia and among the countries located in the international coffee world, because Indonesia is the third largest coffee exporter after Brazil and Vietnam. Coffee production in Indonesia reached an average of 11.250 tonnes per year. The purpose of this study is studying the ability of pyraclostrobin on coffee fertilization, thereby reducing the occurrence of fruit drop and determining the appropriate concentrations for spraying treatment pyraclostrobin on fertilization and reduce fruit drop robusta coffee. This study was conducted in January-September 2016 in the coffee plantation Tlogosari, Tirtoyudo subdistrict, Malang regency which is located at the altitude of 560 m above sea level. The method that used in this study is a randomized block design, consisting of 6 treatments and 4 replications. While the treatment is P0 (without spraying), P1 (Spraying once a week with a concentration of 150 ppm), P2 (Spraying once a week with a concentration of 300 ppm), P3 (Spraying once a week with a concentration of 450 ppm), P4 (Spraying once a week  with a concentration of 600 ppm), P5 (Spraying once a week with a concentration of 750 ppm). Giving pyraclostrobin with a concentration of 600 ppm may increase the amount of fruit compared to the treatment without giving pyraclostrobin.


REVISTA FIMCA ◽  
2018 ◽  
Vol 5 (2) ◽  
pp. 28-31
Author(s):  
Darlan Darlan Sanches Barbosa Alves ◽  
Victor Mouzinho Spinelli ◽  
Marcos Santana Moraes ◽  
Carolina Augusto De Souza ◽  
Rodrigo da Silva Ribeiro ◽  
...  

Introdução: O estado de Rondônia se destaca como tradicional produtor de café, sendo o segundo maior produtor brasileiro de C. canephora. No melhoramento genético de C. canephora, a seleção de plantas de elevada peneira média está associada à bebida de qualidade superior. Objetivos: O objetivo desse estudo foi avaliar a variabilidade genética de clones de C. canephora para o tamanho dos grãos, mensurado a partir da avaliação da peneira média (PM). Materiais e Métodos: Para isso, foi conduzido ao longo de dois anos agrícolas experimento no campo experimental da Embrapa no município de Ouro Preto do Oeste-RO, para a avaliação da peneira média de 130 genótipos (clones) com características das variedades botânicas Conilon, Robusta e híbridos intervarietais. O delineamento experimental utilizado foi de blocos ao acaso, com quatro repetições de quatro plantas por parcela. Resultados: Não houve resultados significativos para a interação clones X anos, indicando uma maior consistência no comportamento das plantas ao longo do tempo. Porém foram observadas diferenças significativas para o tamanho dos grãos entre os genótipos avaliados, possibilitando selecionar genótipos superiores. Conclusão: Os genótipos agruparam-se em cinco classes de acordo com o teste de média, subsidiando a caracterização de um gradiente de variabilidade da característica avaliada ABSTRACTIntroduction: Coffea canephora accounts for approximately 35% of the world's coffee production. The state of Rondônia stands out as a traditional coffee producer, being the second largest Brazilian producer of C. canephora. In the classical genetic improvement of C. anephora, the selection of plants of high average sieve is associated with a drink of superior quality. Objectives: The objective of this udy was to evaluate the genetic variability of Coffea canephora clones for the agronomic medium sieve (PM). Materials and Methods: The experiment was conducted in the experimental field of Embrapa, municipality of OuroPreto do Oeste-RO, located at coordinates 10º44'53 "S and 62º12'57". One hundred thirty genotypes (clones) of botanical characteristics Conilon, Robusta and intervarietal hybrids were evaluated in the agricultural years 2013-2014 and 2014-2015. The experimental design was a randomized block design with four blocks and four plants per plot, spacing 3.5 x 1.5 meters between plants. Results: Significant difference was found for the grain size. According to the F test, at 5% probability, the genotypes were grouped into five classes according to the mean test. Conclusion: The results obtained subsidized the characterization of a variability gradient of the evaluated trait.


2018 ◽  
Vol 48 (4) ◽  
Author(s):  
Leilane Nicolino Lamarão de Oliveira ◽  
Rodrigo Barros Rocha ◽  
Fábio Medeiros Ferreira ◽  
Victor Mouzinho Spinelli ◽  
André Rostand Ramalho ◽  
...  

ABSTRACT: The objective of this research was to quantify the genetic divergence of potential Coffea canephora parents, with the goal of developing progenies that associate the best traits of the Conilon and Robusta botanical varieties for hybrid vigor expression. Thus, 10 morphological and productive characteristics of 130 clones of Conilon and Robusta botanical varieties and their intervarietal hybrids were evaluated over 2 years. The experiment was conducted in a randomized block design with four replicates of four plants per plot. For selection of parents, the main component analysis was used to associate values with reference points obtained from the average of each botanical variety. The first two principal components allowed for the separation of the botanical varieties representing the variability contained in the original data with 76% for the first year and 69% for the second year. Although, the genotype × years interaction had significant effects, there were minor differences in the grouping from one year to the next, which is associated with the higher repeatability estimates observed in this study. It was observed that crosses with the 16-1-81I, 9-1-82L, and 13-1-61I parents of the botanical variety Robusta and the 167I, 890E, and 130I parents of the Conilon botanical variety presented greater potential for obtaining selection gains.


GANEC SWARA ◽  
2020 ◽  
Vol 14 (1) ◽  
pp. 544
Author(s):  
ANAK AGUNG GEDE PUTRA ◽  
PANDE GEDE GUNAMANTA ◽  
KETUT TURAINI INDRA WINTEN

The study aimed to determine the effect of organic planting media and the position of seed planting on the growth of Robusta coffee seedlings. The study is conducted at the Tabanan Agrotechnology Study Program, Tabanan University, which conducted from July 2019 to September 2019. The treatments are arranged in factorial in a randomized block design (RBD). Two treatments examined in this study were the doses of organic matter in the growing media (0g, 150 g, 300 g, 450 g) and seed position (prone position, upside down, tilted position). The results show that the interaction between organic planting media (soil + coffee leaf compost) and seed planting position did not significantly affect all coffee seedlings growth variables. The dose of organic compost in the growing media has a very significant effect on all growth variables except when growing and the percentage of growth. The dosing of 450 g organic matter gave the highest root fresh weight, fresh stem weight and fresh leaf weight (1.00 g, 2.97 g and 8.64 g respectively) and oven dry weight for the three variables (respectively 0.39 g, 0.55 g and 1.36 g). Seed planting position has no significant effect on all observed variables, but there is The tendency of planting position with the prone on average gives better growth compared to other positions.


2020 ◽  
Vol 22 (2) ◽  
pp. 70-78
Author(s):  
Arum Saputri ◽  
Loekas Soesanto ◽  
Abu Umayah ◽  
Agus Sarjito

[EXPLORATION AND VIRULENT TEST OF MAIZE ENDOPHYTE Bacillus sp. AGAINST MAIZE SHEATH BLIGHT]. Capability of endophytic bacterial, effectivity, and its effect on R. solani. and on maize seedlings growth were investigated from April 2018 to January 2019. Exploration of endophytes bacteria in maize was taken from Banyumas Regency (Sumbang, Kembaran, Baturraden) and Purbalingga Regency (Padamara, Bojongsari, Pratin). Taking plant samples using Purposive Random Sampling and Diagonal Sampling methods. Completely randomized design was used in in vitro test with 16 treatments repeated twice. Completely randomized block design was used in in planta experiment with 5 treatments repeated 5 times. The treatment consisted of control, fungiside (mankozeb), and 2 isolates of endophytes bacteria performing the best in vitro result. Variables observed included characteristics of endophytic bacteria and pathogenic fungi, inhibition diameter, incubation period, disease intensity, incidence of disease, AUDPC, plant height, leaf number, root length, plant fresh weight, canopy fresh weight, and root fresh weight. Resultshowed that the exploration obtained 15 endophytic Bacillus sp. isolates. The PD A.4 and BK A.1 isolates werw able to inhibit the growth of pathogenic fungi in-vitro by 56.93 and 51.5%, respectively. The soaking treatment using BK A1 was able to reduce disease intensity by 59.377%, and AUDPC value 34.19%. Endophytic bacteria influence plant height, plant fresh weight, canopy fresh weight, and fresh weight of roots respectively as 89.17 cm, 126.06 g, 106.67 g and 19.4 g.


Irriga ◽  
2021 ◽  
Vol 1 (3) ◽  
pp. 506-516
Author(s):  
Lucas Rosa Pereira ◽  
Edvaldo Fialho dos Reis ◽  
Matheus Gaspar Schwan ◽  
Wilian Rodrigues Ribeiro ◽  
Maria Christina Junger Delôgo Dardengo ◽  
...  

TAMANHO DE GRÃOS DO CAFEEIRO CONILON IRRIGADO E NÃO IRRIGADO DURANTE QUATRO SAFRAS1     LUCAS ROSA PEREIRA2; EDVALDO FIALHO DOS REIS2; MATHEUS GASPAR SCHWAN 2; WILIAN RODRIGUES RIBEIRO2, MARIA CHRISTINA JUNGER DELÔGO DARDENGO3 E SAMUEL FERREIRA DA SILVA4   1 Trabalho extraído da Tese de Doutorado do primeiro autor 2 Departamento de Engenharia Rural, Centro de Ciências Agrárias e Engenharias da Universidade Federal do Espírito Santo - UFES, Alto Universitário S/N, Bairro Guararema, CEP: 29.500.000, Alegre, ES, Brasil, [email protected]; [email protected]; [email protected]; [email protected]. 3 Setor de Cafeicultura, Instituto Federal do Espírito Santo - Campus Alegre – IFES, Rodovia ES-482, km 47 Distrito de Rive, CEP: 29.500.000, Alegre, ES, Brasil, [email protected]. 4 Departamento de Agronomia, Centro de Ciências Agrárias e Engenharias da Universidade Federal do Espírito Santo - UFES, Alto Universitário S/N, Bairro Guararema, CEP: 29.500.000, Alegre, ES, Brasil, [email protected].     1 RESUMO   A pesquisa foi desenvolvida com o objetivo de verificar o tamanho dos grãos do cafeeiro conilon irrigado e não irrigado, durante quatro safras, por meio da classificação física por peneiras. O experimento foi instalado em novembro de 2013 no Instituto Federal do Espírito Santo, Campus Alegre, em esquema de parcela subdividida 3 x 4, sendo nas parcelas o fator manejo de irrigação em três níveis (irrigado com reposição de 100% da evapotranspiração da cultura (ETc), irrigado com 50% da ETc e sem irrigação) e nas subparcelas o fator safra em quatro níveis (2013, 2014, 2015 e 2016), em um delineamento em blocos casualizados, com três repetições. Foi avaliado o percentual de grãos retidos em peneira 13 e superiores, grãos moca e grãos retidos no fundo do conjunto de peneiras. A irrigação influenciou positivamente no tamanho dos grãos. Plantas irrigadas com 100% da ETc obtiveram 72,5% de grãos retidos em peneiras 13 e superiores, já plantas sem irrigação obtiveram 33,1%. Nas safras de 2013 e 2014, foi observado maiores valores de grãos retidos em peneiras 13 e superiores e menores valores de fundo, 76,1% e 7,5%, respectivamente.   Palavras-chave: Coffea canephora, cafeicultura sustentável, déficit hídrico, manejo de irrigação.     PEREIRA, L. R.; REIS, E. F.; SCHWAN, M. G.; RIBEIRO, W. R.; DARDENGO, M. C. J. D.; SILVA, S. F. SIZE OF CONILON COFFEE BEANS IRRIGATED AND NON-IRRIGATED DURING FOUR HARVESTS     2 ABSTRACT   The research was conducted with the objective of verifying the grain size of irrigated and non-irrigated conilon coffee during four harvests, through the physical classification by sieves. The experiment was installed in the year of 2013 in the Instituto Federal do Espírito Santo (Federal Institute of Espírito Santo), in a subdivided-plots scheme 3 x 4, being the plots the factor irrigation management in three levels (irrigated with replacement of 100% of the crop evapotranspiration (ETc), irrigated with 50% of the ETc, and non-irrigated), and in the subplots, the harvest factor in 4 levels (2013, 2014, 2015, and 2016), in a randomized block design, with three replications. The percentage of grains retained in sieve 13 and above, mocha grains, and grains retained at the fund of the sieve set were evaluated. Irrigation had a positive influence on grain size. Plants irrigated with 100% of the ETc obtained 72.5% of grains retained in sieves 13 and higher, while plants without irrigation obtained 33.1%. In the 2013 and 2014 harvests, higher values ​​of grains retained in sieves 13 and higher and lower bottom values, 76.1% and 7.5%, respectively, were observed.   Keywords: Coffea canephora, sustainable coffee growing, water deficit, irrigation management.


2018 ◽  
Vol 9 (1) ◽  
pp. 41-49
Author(s):  
Shabana Askari ◽  
Hina Khurshid

Present work was planned to investigate the improvement in photosynthetic pigments of Cicer arietinum by Conocarpus lancifolius growing under copper stress. Conocarpus lancifolius was chosen as a biosorbent because it is abundantly and easily available plant of Province Sindh. It does not require so much efforts or money or even water to cultivate them, moreover, it is a fast grower. Plants were grown in randomized block design in triplicates. Each pot was supplied with 1kg of soil, the control plants were supplied with distilled water only, experimental plants were supplied with different concentration of copper viz. 0, 25, 50, 75 and 100 ppm whereas treated plants were supplied with 5gm Conocarpus lancifolius leaf powder along with the different concentration of copper. Effect of Copper on Cicer arietinum and its biosorption by Conocarpus lancifolius was observed by the determination of photosynthetic pigments (chlorophyll a, b, total chlorophylls and carotenoids) and its products (glucose, sucrose and total soluble sugars). Various parameters were also analyzed to correlate and confirm our results like % germination, root and shoot lengths, leaf area and relative water content. C. lancifolius was found not only an efficient, eco-friendly and cost-effective biosorbent for heavy metal copper but it is also an effective fertilizer too.


2021 ◽  
pp. 1074-1080
Author(s):  
Daniel Soares Ferreira ◽  
Ramon Amaro de Sales ◽  
José Francisco Teixeira do Amaral ◽  
João Marcos Soares Ferreira ◽  
Luan Peroni Venancio ◽  
...  

Modern agriculture values the most sustainable and dynamic means of production, which can be promoted through the association between coffee and tree components, aiming at the best use of the land. However, within our understanding of the physiological and biochemical behaviors of the coffee tree, the irradiance limiting conditions are crucial to maximize the potential gains generated in these systems. In this sense, the objective of this study was to evaluate the effects of different levels of irradiance restriction on the physiological and biochemical characteristics of two genotypes of Conilon coffee. The experiment was conducted in a 2 × 3 factorial scheme, corresponding to two Conilon coffee clones and three levels of light intensity restriction (0, 30 and 50%), in a randomized block design with four replications. The synthesis of total chlorophyll b and the leaf N content of the genotypes CL6 and CL12 increased with the reduction of light. On the other hand, there was a reduction in the levels of carbohydrates, amino acids and phenols with the reduction of light intensity. Restricted levels of irradiance caused different changes in the physiological and biochemical characteristics of the studied genotypes, showing genetic divergences between them


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