Therapeutic evaluation of homeopathic treatment for canine oral papillomatosis

Aim: A study was conducted to evaluate the ameliorative potential of homeopathic drugs in combination (Sulfur 30C, Thuja 30C, Graphites 30C, and Psorinum 30C) in 16 dogs affected with oral papillomatosis which was not undergone any previous treatment. Materials and Methods: Dogs affected with oral papillomatosis, which have not undergone any initial treatment and fed with a regular diet. Dogs (total=16) were randomly divided into two groups, namely, homeopathic treatment group (n=8) and placebo control group (n=8). Random number table was used for allocation. Homeopathic combination of drugs and placebo drug (distilled water) was administered orally twice daily for 15 days. Clinical evaluation in both groups of dogs was performed by the same investigator throughout the period of study (12 months). Dogs were clinically scored for oral lesions on days 0, 5, 7, 10, 15, 20, 25, 30, 45, 60, 90, 120, and 150 after initiation of treatment. Results: The homeopathic treatment group showed early recovery with a significant reduction in oral lesions reflected by clinical score (p<0.001) in comparison to placebo-treated group. Oral papillomatous lesions regressed in the homeopathic group between 7 and 15 days, whereas regression of papilloma in the placebo group occurred between 90 and 150 days. The homeopathic treated group was observed for 12 months post-treatment period and no recurrence of oral papilloma was observed. Conclusion: The current study proves that the combination of homeopathy drugs aids in fastening the regression of canine oral papilloma and proved to be safe and cost-effective.

Download Full-text

Epigallocatechin gallate delays the onset of type 1 diabetes in spontaneous non-obese diabetic mice

British Journal Of Nutrition ◽

10.1017/s0007114510004824 ◽

2010 ◽

Vol 105 (8) ◽

pp. 1218-1225 ◽

Cited By ~ 63

Author(s):

Zhuo Fu ◽

Wei Zhen ◽

Julia Yuskavage ◽

Dongmin Liu

Keyword(s):

Type 1 Diabetes ◽

Cell Mass ◽

Epigallocatechin Gallate ◽

Nod Mice ◽

Cost Effective ◽

Treated Group ◽

Control Group ◽

Plasma Insulin Levels ◽

Glucose Lowering Effect

Type 1 diabetes (T1D) results from the autoimmune-mediated destruction of pancreatic β-cells, leading to deficiency of insulin production. Successful islet transplantation can normalise hyperglycaemia in T1D patients; however, the limited availability of the islets, loss of islet cell mass through apoptosis after islet isolation and potential autoimmune destruction of the transplanted islets prevent the widespread use of this procedure. Therefore, the search for novel and cost-effective agents that can prevent or treat T1D is extremely important to decrease the burden of morbidity from this disease. In the present study, we discovered that ( − )-epigallocatechin gallate (EGCG, 0·05 % in drinking-water), the primary polyphenolic component in green tea, effectively delayed the onset of T1D in non-obese diabetic (NOD) mice. At 32 weeks of age, eight (66·7 %) out of twelve mice in the control group developed diabetes, whereas only three (25 %) out of twelve mice in the EGCG-treated group became diabetic (P < 0·05). Consistently, mice supplemented with EGCG had significantly higher plasma insulin levels and survival rate but lower glycosylated Hb concentrations compared with the control animals. EGCG had no significant effects on food or water intake and body weight in mice, suggesting that the glucose-lowering effect was not due to an alteration in these parameters. While EGCG did not modulate insulitis, it elevated the circulating anti-inflammatory cytokine IL-10 level in NOD mice. These findings demonstrate that EGCG may be a novel, plant-derived compound capable of reducing the risk of T1D.

Download Full-text

Early detection of MDR Mycobacterium tuberculosis mutations in Pakistan

Scientific Reports ◽

10.1038/s41598-021-96116-x ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Ayma Aftab ◽

Samia Afzal ◽

Zahida Qamar ◽

Muhammad Idrees

Keyword(s):

Early Detection ◽

Control Program ◽

Cost Effective ◽

Treated Group ◽

Multidrug Resistant ◽

Control Group ◽

Double Mutation ◽

Treatment Regimens ◽

Mdr Tb ◽

Save Energy

AbstractThe result of improper treatment has led to the rise of Multidrug-resistant (MDR) strains. This concern still exists in Pakistan. In order to save energy, time and resources an early detection of resistant cases is imperative. Thus, a treated group of 100 isolates and a control group of 56 untreated isolates were studied. PCR and gene sequencing showed mutations at codon 531 and 513 in the rpoB gene. 12% of cases showed a double mutation in the rpoB gene. katG gene showed mutations at codon 315 and 299. 28.6% of the control group cases were positive for MDR whereas 100% of the treated group were positive for MDR. This study explores the significantly increasing ratio of MDR-TB among Pakistani population. This study provides prevalent MDR mutations among Pakistanis and suggests developing such molecular assays that are time and cost effective. Importance: Pakistan is a developing country and has fourth highest incidence rate of MDR-TB. The treatment of MDR-TB is the use of second line drugs that has severe side effects as well as it requires long time span. One of the strategies to control the spread of MDR-TB is to decipher the aberrations at molecular level in order to formulate potent drugs that can treat the patients within short span of time. Determining the mutation profile of MDR in Pakistani populations will open new horizons for the improvement of drug treatment regimens to make it more effective or for the development of novel potent drugs and vaccines to better treat the drug-resistant TB. Moreover, this study will be help in disease control program.

Download Full-text

Cost-effectiveness of allergic rhinitis treatment: An exploratory study

SAGE Open Medicine ◽

10.1177/2050312118794588 ◽

2018 ◽

Vol 6 ◽

pp. 205031211879458

Author(s):

Jan Titulaer ◽

Habibollah Arefian ◽

Michael Hartmann ◽

Mustafa Z Younis ◽

Orlando Guntinas-Lichius

Keyword(s):

Allergic Rhinitis ◽

Cost Effectiveness ◽

Side Effects ◽

Symptom Score ◽

Treatment Options ◽

Local Therapy ◽

Cost Effective ◽

Subcutaneous Immunotherapy ◽

Control Group ◽

Placebo Control

Allergic rhinitis is serious public health problems and one of the most common chronic diseases worldwide. We aimed to assess the cost-effectiveness of clinically relevant treatment options for allergic rhinitis using evidence-based literature. In addition, we aimed to develop recommendations for allergic rhinitis treatment based on health economic facts. We searched MEDLINE via PubMed from 2009 to 2014 to identify all therapeutic options described in the current literature and selected randomized controlled trials that used a symptom score, had at least one placebo control group and used adult patients. We analyzed the side effects and the number of cases in which treatment was discontinued for each treatment option. Local antihistamines were the most cost-effective local therapy and are recommended due to the low number of complications. Regarding systemic therapies, although the use of oral steroids is indeed significantly cost-effective, this treatment was found to be associated with strong side effects. Sublingual immunotherapy was identified as the most cost-effective immunotherapy and exhibits a good side-effect profile. Overall, local therapy with antihistamines was found to be the most cost-effective option of all therapies. This study showed that there are only minor differences between sublingual and subcutaneous immunotherapy. Based on our results, we recommend the use of an international, uniform nasal symptom score to facilitate the comparison of clinical trials on allergic rhinitis in the future.

Download Full-text

The Femoral Prosthesis Fracture Analysis around Femur after Hip Replacement

Advanced Emergency Medicine ◽

10.18686/aem.v4i4.15 ◽

2015 ◽

Vol 4 (4) ◽

pp. 45

Author(s):

Yuehao Xu

Keyword(s):

Treatment Group ◽

Hip Replacement ◽

Locking Plate ◽

Plate Fixation ◽

Healing Process ◽

Treated Group ◽

Control Group ◽

Clinical Effects ◽

Femoral Prosthesis ◽

Locking Plate Fixation

Objective: To investigate the clinical effects of patients with femoral prosthesis fracture phenomena after the application of locking plate fixation for hip replacement surgery. Method: 78 cases of postoperative patients with periprosthetic femoral fracture phenomenon after hip replacement were selected and divided randomly into a control and treatment group, respectively, with an average of 39 cases per group. The control group was treated with the shape memory alloy inner ring fixing technology whereas the locking plate fixation was applied for the treatment group. Result: Fracture treatment around the femoral prosthesis in patients of treated group was better in comparison to the control group. Significantly, the treatment group revealed shorter duration of hospitalization, the recovery of limb weight-bearing capacity and the healing process as compared to the control group. Harris scores were significantly higher in the treated group in contrast to the control group. Additionally, lower adverse reaction rate was found in the treatment group as compared to the control group. Conclusion: Obviously, patient who have femoral periprosthetic fracture phenomena after receiving hip replacement were likelihood to be treated with the locking plate fixation technique.

Download Full-text

Forced Suppression of let-7a-5p in Mouse Blastocysts Improves Implantation Rate

10.21203/rs.3.rs-179928/v1 ◽

2021 ◽

Author(s):

Jalleh Shakerzadeh ◽

Mansoureh Movahedin ◽

Akram Eidi ◽

Nasim Hayati Roodbari ◽

Kazem Parivar

Keyword(s):

Treatment Group ◽

Embryo Implantation ◽

Implantation Rate ◽

Treated Group ◽

Control Group ◽

Reverse Transcription Pcr ◽

Regulated Expression ◽

Significant Expression ◽

And Control ◽

Control Sham

Abstract BackgroundEmbryo implantation requires appropriate communication between the blastocyst and endometrium. Recurrent implantation failure is an essential component of assisted reproductive technology. Also, miRNAs-mediated gene expression impacts the implantation process, and the down-regulation of some miRs, such as mmu-let-7a, improves this process. In the present study, we evaluated the effect of let-7a forced-suppression on the mouse implantation rate. MethodsIn total, 100 adult female mice and 10 adult male mice were included (Strain CD-1). We analysed the expression of let-7a and its potential mRNAs targets (Igf1, Il1a, Itgb3, and Tgfb1) in control, sham, and antagomir-treated blastocysts using quantitative reverse transcription PCR (qRT-PCR). The control and treated blastocysts were transferred to the 20 pseudopregnant mice so that the effect of let-7a suppression on the rate of implantation could be determined.ResultsThe expression level of let-7a in the treatment group was significantly down-regulated (P=0.001) In contrast, no significant expression changes were observed for let-7a or mRNAs targets when the sham and control groups were compared (P>0.05). In comparison to the controls, the antagomir-treated group exhibited significantly up-regulated expression levels of Igf1 (0.0167), Itgb3 (0.045), and Tgfb1 (0.0115). Additionally, the implantation rate was significantly higher in the treatment group (78%) than the control group (61%) (P=0.0098).ConclusionWe found that forced-suppression of mmu-let-7a-5p through successful transfection of Anti-miR leads to upregulation of downstream genes, Igf1, Itgb3, and Tgfb1, which directly involved in the trophoblast- endometrium attachment and improve the implantation rate.

Download Full-text

The effects of firocoxib (Previcox™) in geriatric dogs over a period of 90 days

Journal of the South African Veterinary Association ◽

10.4102/jsava.v80i3.198 ◽

2009 ◽

Vol 80 (3) ◽

Cited By ~ 3

Author(s):

K.E. Joubert

Keyword(s):

Treatment Group ◽

Bile Acids ◽

Adverse Drug Events ◽

High Standard ◽

Treated Group ◽

Mean Value ◽

Urine Specific Gravity ◽

Control Group ◽

High Risk Population ◽

Group A

The long-term use of non-steroidal anti-inflammatory agents in geriatric dogs with osteoarthritis has not been well studied in veterinary medicine. This study evaluated the effects of firocoxib administered to dogs over 7 years of age for 90 days. Pain and lameness scores were evaluated by the owner weekly for the 1st month and then biweekly through to the end of the study, the veterinarian evaluated the dogs monthly. Serum chemistry, including urea, creatinine, alanine transferase, aspartate transaminase, bile acids and bilirubin, urine specific gravity and a urine dipstick, were performed at monthly intervals. Forty-five dogs were enrolled into the treatment group and 9 into the control group. A total of 33 dogs completed the trial in the treatment group and 8 in the control group. Lameness and pain scores were found to be significantly lower in the treated group from day 30 for most parameters evaluated. Bile acids (although not comparable to controls, with higher mean value and a high standard deviation in the control group; in addition the control group had increased bile acids at day 0) and urea (within normal reference range provided (WNL)) were significantly different in the treatment group between days 0 and 90. Urea (WNL) on days 30 and 90 and creatinine (WNL) on day 90 were significantly different between the control group and the treatment group. The most common adverse events reported were diarrhoea, vomition, dark faeces and anorexia. This study showed that firocoxib was effective in managing pain associated with osteoarthritis for 90 days. Despite the geriatric high-risk population used for this study, minimal biochemical changes were seen and adverse drug events seen were in agreement with those previously reported.

Download Full-text

THE ROLE OF HEPARIN IN TREATMENT OF EXPERIMENTAL PERITONITIS

10.36106/gjra/0800937 ◽

2019 ◽

pp. 1-5

Author(s):

Pradeep Kumar Saxena

Keyword(s):

Single Dose ◽

Survival Rate ◽

Treatment Group ◽

Normal Saline ◽

Adhesion Formation ◽

Treated Group ◽

Repeated Dose ◽

Control Group ◽

Albino Rats ◽

Bowel Loop

Background: To produce peritonitis experimentally in albino rats by creation of a necrotic loop of terminal Ileum. Study of peritonitis and gross changes in peritoniteal cavity and to study the effect of single dose heparin (Anticoagulant) in experimentally produced peritonitis. Also to study and compare the effect of repeated small dosage of heparin in peritonitis. Material and Methods The rats were divided into 6 groups, under the 2 experiments. So each group comprised of 8 rats. The peritonitis was proceduced by Rasto's method, in which the peritonitis was caused by a gangrenous loop of small intestine . two types of experiment were carried out: 1. Experiment 1 : The gangrenous loop which produced peritonitis was excised after 24 hours, normal saline was given in control group,whereas heparin as a single dose and heparin in small repeated dose were given by sub-cutaneous or intraperitoneal route for 3 days. No abdominal toilet or antibiotics were given during the time. The surviving as well as the dead rats during observation period were subjected to laparotomy and detailed pathology of peritoneum was studied. 2. Experiment 2 : In this group the gangrenous loop was not resected after 24 hours and normal saline was given in control group 0.2 ml., or heparin in a single dose 50 I.U. or heparin in small repeated dose 20 I.U. twice a day for three days.All the rats were continuously observed during the post-operative period for evidence of any complications. Results: The peritonitis produced by a necrotic bowel loop was severe & brino-purulent.The formation of inter-mesentric abscess in control group was much more evident than the heparinised rats. The size of inter-mesentric abscess was smaller in treated group of albino rats than in control.The incidence of adhesion formation was much more in control group, than in the heparin group, the adhesion were very less friable and easily breakable. The survival rate in heparin treatment group was 75% to 87.5% as compared to the control group, where the survival rate was 50% only.The mortality in the control group, where the necrotic loop, was not resected was as high as 87.5% and rats died within 8 days after operation whereas the mortality rate in treatment group was low that is, from 50% to 60% only. Conclusion: By comparison and contrast of the results of the difference treatment group, it become evident that survival in the treated group was signicantly better than control group. About the evidence of intra-peritoneal infection, it shows that in treated group clearance of peritonitis was much faster than the control group of albino rats:The number and size of intramesentric abscesses were also smaller in treated group. There was also a little benecial effect on adhesion in heparinised albino rats as compared to control group.So heparin in small repeated doses has denitely a signicant effect on secondary bacterial peritonitis and its subsequent results.

Download Full-text

COGNITIVE THERAPY GROUP DYNAMICS MODEL FOR REDUCING THE LEVEL OF DEPRESSED PATIENTS IN MENTAL HOSPITAL

INDONESIAN NURSING JOURNAL OF EDUCATION AND CLINIC (INJEC) ◽

10.24990/injec.v1i2.66 ◽

2018 ◽

Vol 1 (2) ◽

pp. 131

Author(s):

Arum Pratiwi

Keyword(s):

Treatment Group ◽

Cognitive Therapy ◽

Group Dynamics ◽

Therapy Group ◽

Treated Group ◽

Control Group ◽

Mild Depression ◽

Depressed Patients ◽

Moderate Depression ◽

Post Test

Introduction. The worst impact of depression is suicide, it need comprehensive management. Model of cognitive therapy through group dynamics try to break the chain, so, that the body does not respond to stimuli that lead to increased serotonin. The purpose of this study was create a new model of nursing care, describe the personal characteristics of depressed patients, describe of the level of depressed patients and compare depressed patients between the control group and the treatment group. Method. The design of this study was quasi experiments with pre test and post test control group. The sample study used purposive sampling strategies, that was patients in mental hospitals and selected with screening and than they were chosen with mild and moderate categories. The samples were 32 patients, 16 patients were a control group and 16 patients were a treated group. Patients were given a therapy model for one month which is 2 times a week. After that the level of depression were measured by BECK questionnaires. The data were analyzed by using independent t test. Result. The results showed that the distribution of the level of depression at pretest, 62.5% of mild depression in the treatment group and 62.5% of moderate depression in the control group. The result of post test, the mild depression decreased to 25%, and the moderate depression to 37.5%. The t test results concluded that the two groups there was difference in rates of depression between the control group and the treated group with t value significance 7.000 and 0.000. Discussion. In future studies it would be better if the treatment done in the longer term and depression levels were analyzed periodically. Keywords: Cognitive Therapy, Group Dynamics, Depressed Patients

Download Full-text

Efficacy of a novel topical combination of esafoxolaner, eprinomectin and praziquantel against Echinococcus multilocularis infections in cats

Parasite ◽

10.1051/parasite/2021025 ◽

2021 ◽

Vol 28 ◽

pp. 29

Author(s):

Joe Prullage ◽

Dwight Bowman ◽

Michael Ulrich ◽

Eric Tielemans

Keyword(s):

Echinococcus Multilocularis ◽

Experimental Studies ◽

The United States ◽

Treated Group ◽

Control Group ◽

Placebo Control ◽

The Novel ◽

Control Groups ◽

Geometric Means ◽

Placebo Control Group

NexGard® Combo, a novel topical endectoparasiticide formulation for cats combining esafoxolaner, eprinomectin and praziquantel, for the treatment of internal and external parasite infestations, including arthropods, nematodes and cestodes, was tested for efficacy against induced infections of Echinococcus multilocularis in cats, in two experimental studies. The two studies were performed in the United States with the same E. multilocularis isolate sourced locally. In each study, 20 cats were inoculated intra-gastrically with ~30,000 E. multilocularis protoscoleces three weeks before treatment, then ten cats were randomly allocated to a placebo control group or to the novel formulation treated group. Inoculated cats were treated topically once at the minimum recommended dose of the novel formulation, or with an identical volume of placebo. One week after treatment, cats were humanely euthanized for parasite recovery and count. The efficacy calculation was based on comparison of number of scoleces found in the control group and the novel formulation group. In the two control groups, E. multilocularis scoleces were found in five (range: 30–1025) and eight (range 2–345) cats, the geometric means inclusive of the ten cats per group were 8.9 and 28.8, respectively. In the two novel formulation-treated groups, none of the cats harbored any E. multilocularis scoleces, demonstrating 100% efficacy.

Download Full-text

11 FERTILITY RESPONSE IN SUCKLED BEEF COWS SUPPLEMENTED WITH LONG-ACTING PROGESTERONE AFTER TIMED ARTIFICIAL INSEMINATION

Reproduction Fertility and Development ◽

10.1071/rdv27n1ab11 ◽

2015 ◽

Vol 27 (1) ◽

pp. 98 ◽

Cited By ~ 2

Author(s):

G. Pugliesi ◽

F. B. Santos ◽

E. Lopes ◽

É. Nogueira ◽

J. R. G. Maio ◽

...

Keyword(s):

Prostaglandin F2α ◽

Beef Cows ◽

Treated Group ◽

Control Group ◽

Placebo Control ◽

Negative Effects ◽

Group V ◽

Buserelin Acetate ◽

Long Acting ◽

To Receive

Corpus luteum (CL) and progesterone (P4) secretion are affected by preovulatory follicle (POF) size. Increased circulating P4 during early diestrus has a positive effect on embryo development in beef cattle. However, the combined effects of the POF size and P4 supplementation during early diestrus on fertility of beef cows are not known. The objective was to evaluate the effects of POF size and supplementation of long-acting P4 after timed-AI on pregnancy rates (P/AI). Suckled Nelore cows (n = 596) were evaluated twice by transrectal Doppler ultrasonography (US) 10 days apart to detect the cyclic status. In Study 1, anestrous cows (absence of CL on both exams) received an intravaginal P4 device and an oestradiol benzoate (EB) injection on Day –10 (day of the second US). Devices were removed and sodium cloprostenol [prostaglandin F2α (PGF2α)], oestradiol cypionate, and eCG were given on Day –2. Cows were timed-AI on Day 0 and assigned to receive placebo (control group, n = 187) or 150 mg of long-acting P4 on Day 4 (P4 group, n = 189). In Study 2, cyclic cows (presence of CL) received a PGF2α injection on Day –20 (first US). Cows with a new CL on Day –10 received an intravaginal P4 device and an injection of EB and were split to receive an injection of PGF2α [large follicle (LF); n = 109] or not [small follicle (SF); n = 111]. Devices were removed and PGF2α was injected on Day –2. Ovulation was induced with buserelin acetate, and cows were timed-AI on Day 0 and split to receive placebo (LF/control group, n = 55, and SF/control group, n = 55) or 150 mg of long-acting P4 on Day 4 (LF/P4 group, n = 56, and SF/P4 group, n = 54). Ultrasonographic scanning was done on Days 0, 4, and between 35 and 40 to detect the POF and CL sizes and P/AI, respectively. Data were analysed using PROC GLIMMIX (SAS Institute Inc., Cary, NC). In anestrous cows, P/AI was reduced in POF with <11 mm. The P/AI was greater in the P4-treated group than in the control group for all cows (55.6% v. 46.0%; P = 0.05) and for ovulated cows (59%, 105/178 v. 49%, 86/173; P = 0.08). For cyclic cows, POF size (mm) on Day 0 (13.5 ± 0.3 v. 11.2 ± 0.2), ovulation rate (90% v. 77%), and CL area (cm2) on Day 4 (1.46 ± 0.05 v. 1.25 ± 0.05) were greater (P < 0.007) in the LF group than in the SF group. There was a main effect of follicle group on P/AI (54%, LF group v. 38%, SF group; P < 0.01). Moreover, P/AI were greater (P < 0.05) in the LF/control (56%) and LF/P4 (52%) groups than in the SF/control group (31%), whereas no difference was detected between the SF/P4 group (45%) and the other groups. Among cows that ovulated, P/AI was lower (P = 0.05) in the SF/control group (41%, 17/41) compared with the LF/control group (62%, 31/50) and were similar for the SF/P4 group (56%, 25/45) and LF/P4 group (57%, 28/49) compared with others. We suggest that P4-stimulated embryotrophic effects improved fertility in anestrous beef cows supplemented with long-acting P4 on Day 4 after timed-AI. Also, the presence of a functional CL during follicle growth results in smaller POF and CL and reduces the ovulatory and P/AI rates in cyclic cows. Post-AI P4 supplementation may attenuate the negative effects of small POF/CL. Research was supported by CNPq, FAPESP, Ouro Fino Agronegócio, and Innovare.

Download Full-text