Extraction Process and Analysis of Components in Essential Oils of
Piper longum Linn. Harvested in Dak Lak Province, Vietnam

Nowadays, plants containing biological activities are increasingly interested for their antioxidant and antibacterial properties. In particular, Pepper longum Linn. was known as one of the popular types of pepper family in Vietnam with many different uses in food and medicine. In this study, Pepper longum fruits were studied for extraction to essential oil by hydrodistillation through two survey methods such as single factor investigation method and optimization by response surface methodology (RSM). The analysis results by design expert software program version 11 shown that at the condition that the ratio of water to raw material 6:1 (mL/g), during 225 min extraction at 130 ºC, the essential oil yield was obtained 0.8%. The model predicts this result for F-value, P-values and Lack of Fit are mathematically significant and values for reliability R2 = 0.9963, C.V. % =1.99. Essential oil obtained was evaluated by gas chromatography-mass spectrometry (GC-MS) technique, which accounted that β-caryophyllene contains the highest amount among the other 43 components present.

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The Study on Extraction Process and Analysis of Components in Essential Oils of Black Pepper (Piper nigrum L.) Seeds Harvested in Gia Lai Province, Vietnam

Processes ◽

10.3390/pr7020056 ◽

2019 ◽

Vol 7 (2) ◽

pp. 56 ◽

Cited By ~ 39

Author(s):

Thien Hien Tran ◽

Le Ke Ha ◽

Duy Chinh Nguyen ◽

Tan Phat Dao ◽

Le Thi Hong Nhan ◽

...

Keyword(s):

Essential Oil ◽

Respiratory Infections ◽

Mesh Size ◽

Extraction Process ◽

Black Pepper ◽

Raw Material ◽

Gas Chromatography Mass Spectrometry ◽

Piper Nigrum ◽

Efficient Technology ◽

Preservation Method

Black pepper (Piper nigrum L.) is a tropical crop with extensive medicinal potential in ethnomedicine and nutraceutical applications. The essential oil of black pepper finds wide applications in inhabitation of respiratory infections and soothing of muscular pains due to its warming and energizing property. The pungent bioactive piperine is responsible for this function, and therefore, efficient technology is required for an optimal extraction process of this compound. In the present article, we have developed a procedure for extracting black pepper essential oil from Vietnam, optimizing conditions that affect the extraction process. The effect of process parameters, namely material size, preservation method, the concentration of sodium chloride, the concentration of soak time, the ratio of material to water, temperature extraction, time extraction on the extraction yield, and relative efficiency were investigated. Results demonstrated that 20 g of black pepper milled with a mesh size of 160 obtained 0.48 g of essential oil (2.4%) at a raw material to water ratio of 1/21 (g/mL) at 150 °C in a time of 5.2 h. GC-MS (Gas chromatography–mass spectrometry) spectra showed that 3-carene (29.21%), D-limonene (20.94%), caryophyllene (15.05%), and β-pinene (9.77%) were present as major components. These results suggested that the essential oil extracted from Vietnamese black pepper is applicable in the manufacturing processes of insecticides and air deodorizers.

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Study on optimization of extraction technique of pericarp essential oil in Litsea Cubeba (Lour) Pers

Journal of Food Measurement & Characterization ◽

10.1007/s11694-020-00657-0 ◽

2020 ◽

Author(s):

Peng Huang ◽

Zhanying Gu ◽

Ling Yang ◽

Ruonan Yang ◽

Yaxin Ji ◽

...

Keyword(s):

Essential Oil ◽

Essential Oils ◽

Extraction Process ◽

Relative Content ◽

Raw Material ◽

Gas Chromatography Mass Spectrometry ◽

Orthogonal Experimental Design ◽

Microwave Pretreatment ◽

Litsea Cubeba ◽

Main Component

Abstract Litsea cubeba is an important aromatic oil plant. Litsea cubeba pericarp essential oil has various physiological activities such as antibacterial activity, antioxidant capacity, etc., and is widely used. Its main ingredient is citral, which is the raw material for the synthesis of many precious spices. For the purpose of increasing yield, researchers have conducted many studies on the extraction of essential oil from pericarp of Litsea cubeba. But the extraction process still has room for optimization。In this study, Litsea cubeba fruits were subjected to an optimized method of hydrodistillation to increase the yield and biological activity of pericarp essential oils. Three-factor and three-level orthogonal experimental design was performed (fruit preservation time, distillation voltage and fruit microwave pretreatment time) to optimize the process. According to the results of orthogonal experiments, the highest yield of essential oil can be obtained after the fruit has been preserved for 9 days without microwave pretreatment and the distillation voltage is 220 V. The extracted essential oils were analyzed by gas chromatography-mass spectrometry (GC–MS), which indicated that their main components were 2,6-Octadienal, 3,7-dimethyl-, (E)-, 2,6-Octadienal, 3,7-dimethyl-, (Z)-, 1-Heptanol and 1-Octanol. GC–MS results showed that the relative content of citral in the extracted essential oil was the highest after the fruit was preserved for nine days without microwave pretreatment and the distillation voltage was 150 V. Changes in the process will not significantly affect the main component types of essential oils, but will significantly affect the relative content of the same components.

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Medicinal Plants: Antibacterial Effects and Chemical Composition of Essential Oil of Foeniculum vulgare

International Journal of Current Pharmaceutical Review and Research ◽

10.25258/ijcprr.v8i01.9082 ◽

2017 ◽

Vol 8 (01) ◽

Cited By ~ 2

Author(s):

Azadeh Foroughi ◽

Pouya Pournaghi ◽

Fariba Najafi ◽

Akram Zangeneh ◽

Mohammad Mahdi Zangeneh ◽

...

Keyword(s):

Chemical Composition ◽

Medicinal Plants ◽

Essential Oil ◽

Antibacterial Properties ◽

Screen Test ◽

Antibacterial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Resistant Bacteria ◽

Antibacterial Effects ◽

Foeniculum Vulgare

Medicinal plants are considered modern resources for producing agents that could act as alternatives to antibiotics in demeanor of antibiotic-resistant bacteria. The aim of the study was to evaluate the chemical composition and antibacterial activities of essential oil of Foeniculum vulgare (FV) against Pseudomonas aeruginosa and Bacillus subtilis. Gas chromatography mass spectrometry was done to specify chemical composion. As a screen test to detect antibacterial properties of the essential oil, agar disk and agar well diffusion methods were employed. Macrobroth tube test was performed to determinate MIC. The results indicated that the most substance found in FV essential oil was Trans-anethole (47.41 %), also the essential oil of FV with 0.007 g/ml concentration has prevented P. aeruginosa and with 0.002 g/ml concentration has prevented B. subtilis from the growth. Thus, the research represents the antibacterial effects of the medical herb on test P. aeruginosa and B. subtilis. We believe that the article provide support to the antibacterial properties of the essential oil. The results indicate the fact that the essential oil from the plant can be useful as medicinal or preservatives composition.

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Identification of VOCs in essential oils extracted using ultrasound- and microwave-assisted methods from sweet cherry flower

Scientific Reports ◽

10.1038/s41598-020-80891-0 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Huimin Zhang ◽

Hongguang Yan ◽

Quan Li ◽

Hui Lin ◽

Xiaopeng Wen

Keyword(s):

Essential Oil ◽

Sweet Cherry ◽

Dimethyl Sulfide ◽

Solid Phase ◽

Oil Yield ◽

Gas Chromatography Mass Spectrometry ◽

Process Conditions ◽

Important Indicator ◽

Microwave Assisted ◽

Essential Oil Yield

AbstractThe floral fragrance of plants is an important indicator in their evaluation. The aroma of sweet cherry flowers is mainly derived from their essential oil. In this study, based on the results of a single-factor experiment, a Box–Behnken design was adopted for ultrasound- and microwave-assisted extraction of essential oil from sweet cherry flowers of the Brooks cultivar. With the objective of extracting the maximum essential oil yield (w/w), the optimal extraction process conditions were a liquid–solid ratio of 52 mL g−1, an extraction time of 27 min, and a microwave power of 435 W. The essential oil yield was 1.23%, which was close to the theoretical prediction. The volatile organic compounds (VOCs) of the sweet cherry flowers of four cultivars (Brooks, Black Pearl, Tieton and Summit) were identified via headspace solid phase microextraction (SPME) and gas chromatography–mass spectrometry (GC–MS). The results showed that a total of 155 VOCs were identified and classified in the essential oil from sweet cherry flowers of four cultivars, 65 of which were shared among the cultivars. The highest contents of VOCs were aldehydes, alcohols, ketones and esters. Ethanol, linalool, lilac alcohol, acetaldehyde, (E)-2-hexenal, benzaldehyde and dimethyl sulfide were the major volatiles, which were mainly responsible for the characteristic aroma of sweet cherry flowers. It was concluded that the VOCs of sweet cherry flowers were qualitatively similar; however, relative content differences were observed in the four cultivars. This study provides a theoretical basis for the metabolism and regulation of the VOCs of sweet cherry flowers.

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Phytochemical Analysis and Potential Biological Activities of Essential Oil from Rice Leaf

Molecules ◽

10.3390/molecules24030546 ◽

2019 ◽

Vol 24 (3) ◽

pp. 546 ◽

Cited By ~ 3

Author(s):

Truong Minh ◽

Tran Xuan ◽

Truong Van ◽

Yusuf Andriana ◽

Tran Viet ◽

...

Keyword(s):

Mass Spectrometry ◽

Essential Oil ◽

Atmospheric Pressure Chemical Ionization ◽

Biological Activities ◽

Methyl Ricinoleate ◽

Gas Chromatography Mass Spectrometry ◽

Phytochemical Analysis ◽

Chemical Compositions ◽

Rice Leaf ◽

Rice Leaves

Although many investigations on phytochemicals in rice plant parts and root exudates have been conducted, information on the chemical profile of essential oil (EO) and potent biological activities has been limited. In this study, chemical compositions of rice leaf EO and in vitro biological activities were investigated. From 1.5 kg of fresh rice leaves, an amount of 20 mg EO was obtained by distillation and analyzed by gas chromatography-mass spectrometry (GC-MS), electrospray ionization (ESI), and atmospheric pressure chemical ionization (APCI) to reveal the presence of twelve volatile constituents, of which methyl ricinoleate (27.86%) was the principal compound, followed by palmitic acid (17.34%), and linolenic acid (11.16%), while 2-pentadecanone was the least (2.13%). Two phytoalexin momilactones A and B were first time identified in EO using ultra-performance liquid chromatography coupled with electrospray mass spectrometry (UPLC/ESI-MS) (9.80 and 4.93 ng/g fresh weight, respectively), which accounted for 7.35% and 3.70% of the EO, respectively. The assays of DPPH (IC50 = 73.1 µg/mL), ABTS (IC50 = 198.3 µg/mL), FRAP (IC50 = 700.8 µg/mL) and β-carotene oxidation (LPI = 79%) revealed that EO possessed an excellent antioxidant activity. The xanthine oxidase assay indicated that the anti-hyperuricemia potential was in a moderate level (IC50 = 526 µg/mL) as compared with the standard allopurinol. The EO exerted potent inhibition on growth of Raphanus sativus, Lactuca sativa, and two noxious weeds Echinochloa crus-galli, and Bidens pilosa, but in contrast, the growth of rice seedlings was promoted. Among the examined plants, the growth of the E. crus-galli root was the most inhibited, proposing that constituents found in EO may have potential for the control of the problematic paddy weed E. crus-galli. It was found that the EO of rice leaves contained rich phytochemicals, which were potent in antioxidants and gout treatment, as well as weed management. Findings of this study highlighted the potential value of rice leaves, which may provide extra benefits for rice farmers.

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Chemical Composition and Antioxidant and Antimicrobial Activities of Wormwood (Artemisia absinthiumL.) Essential Oils and Phenolics

Journal of Chemistry ◽

10.1155/2015/804658 ◽

2015 ◽

Vol 2015 ◽

pp. 1-12 ◽

Cited By ~ 35

Author(s):

Kamel Msaada ◽

Nidhal Salem ◽

Olfa Bachrouch ◽

Slim Bousselmi ◽

Sonia Tammar ◽

...

Keyword(s):

Gas Chromatography ◽

Essential Oil ◽

Essential Oils ◽

Methanolic Extract ◽

Biological Activities ◽

Reducing Power ◽

Antimicrobial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Oil Composition ◽

Antioxidant And Antimicrobial Activities

The aim of this study was to determine the chemical variability of wormwood extracts as affected by the growing region. Antioxidant and antimicrobial activities were also investigated. The essential oil composition variability ofA. absinthiumL. aerial parts collected from four different Tunisian regions was assessed by gas chromatography (GC/FID) and by gas chromatography mass spectrometry (GC/MS). In addition, total polyphenols, flavonoids, and condensed tannins as well as antioxidant, antibacterial, and antifungal activities of methanolic extract and essential oils were undertaken. Chromatographic analysis of wormwood essential oils showed the predominance of monoterpene hydrocarbons represented mainly by chamazulene. RP-HPLC analysis of wormwood methanolic extract revealed the predominance of phenolic acids. Antiradical activity was region-dependant and the methanolic extract of Bou Salem region has the strongest activity (CI50=9.38±0.82 µg/mL). Concerning the reducing power, the methanolic extract of Bou Salem, Jérissa, and Boukornine regions was more active than the positive control. Obtained results of antimicrobial activities showed that wormwood essential oil is endowed with important antibacterial activity which was strongly related to the organoleptic quality of oil which appeared strongly region-dependant.A. absinthiumL. EOs investigated are quite interesting from a pharmaceutical standpoint because of their biological activities.

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In Vitro Study of Multi-Therapeutic Properties of Thymus bovei Benth. Essential Oil and Its Main Component for Promoting Their Use in Clinical Practice

Journal of Clinical Medicine ◽

10.3390/jcm7090283 ◽

2018 ◽

Vol 7 (9) ◽

pp. 283 ◽

Cited By ~ 8

Author(s):

Sherif Hassan ◽

Kateřina Berchová-Bímová ◽

Miroslava Šudomová ◽

Milan Malaník ◽

Karel Šmejkal ◽

...

Keyword(s):

Gas Chromatography ◽

Clinical Practice ◽

Essential Oil ◽

Biological Activities ◽

Structural Parameters ◽

Antihypertensive Agents ◽

Gas Chromatography Mass Spectrometry ◽

Ic50 Values ◽

Main Component

Thymus bovei Benth. (TB) is an important plant in the traditional medicine of the Mediterranean region. This study investigates the health-promoting properties of TB essential oil (TB-EO) for its possible use in clinical practice with regards to its cytotoxic, anti-herpes simplex virus type 2 (HSV-2), and antihypertensive (through inhibition of human angiotensin-converting enzyme; ACE) properties. The phytochemical profile of EO (99.9%) was analyzed by Gas Chromatography with Flame-Ionization Detection (GC-FID) and Gas Chromatography-Mass Spectrometry (GC-MS). In this study, all biological methods were performed at the level of in vitro studies. The results showed that TB-EO exerted remarked cytotoxic properties against human cervical carcinoma cells, colon cancer cells, and lung adenocarcinoma cells with the half-maximal inhibitory concentration (IC50) values of 7.22, 9.30, and 8.62 µg/mL, respectively, in comparison with that of standard anticancer drug cisplatin with IC50 values of 4.24, 5.21, and 5.43 µg/mL, respectively. Fascinatingly, TB-EO showed very weak cytotoxicity on the healthy human fetal lung fibroblast cells with an IC50 value of 118.34 µg/mL compared with that of cisplatin (IC50 = 10.08 µg/mL). TB-EO, its main component geraniol, TB-EO combined with acyclovir (ACV) along with standard ACV, have displayed pronounced inhibitory properties against the replication of HSV-2 with the half-maximal effective concentration (EC50) values of 2.13, 1.92, 0.81 and 1.94 µg/mL, respectively, with corresponding selectivity indices (SI) 98.59, 109.38, 259.26 and 108.25, respectively. TB-EO and geraniol at a concentration of 15 µg/mL showed prominent inhibitory activities against ACE with % of inhibition 95.4% and 92.2%, respectively, compared with that of standard inhibitor captopril (99.8%; 15 µg/mL). Molecular docking studies were performed to unveil the mechanism of action of geraniol as well as structural parameters necessary for anti-HSV-2 activity (through the inhibition of HSV-2 protease) and ACE inhibition. This is the first report on the chemical composition of Egyptian TB-EO along with the above-mentioned biological activities. Our results may be considered as novel findings in the course of a search for new and active anticancer, anti-HSV-2 and antihypertensive agents, and expand the medicinal value of this plant and its phytochemicals in clinical practice.

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Chemical composition and antibacterial activities of essential oils from fruits of Melicope pteleifolia (Champ. Ex Benth.) T.G. Hartley grown in Lam Dong Province, Vietnam

ACADEMIA JOURNAL OF BIOLOGY ◽

10.15625/2615-9023/v42n3.13916 ◽

2020 ◽

Vol 42 (3) ◽

Author(s):

Hoang Thi Binh ◽

Tran Thi Bao Tram ◽

Do Ngoc Dai ◽

Vuong Thuy Tien ◽

Le Minh Tam ◽

...

Keyword(s):

Mass Spectrometry ◽

Chemical Composition ◽

Essential Oil ◽

Streptococcus Pyogenes ◽

Antibacterial Properties ◽

Antibacterial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Diffusion Method ◽

Aerial Parts ◽

Agar Well Diffusion Method

In the present study, chemical composition and antibacterial properties of essential oil obtained from the aerial parts of the Melicope pteleifolia (Champ. ex Benth.) T.G Hartley in Dalat were evaluated. Essential oil was isolated through hydro-distillation. Twenty-nine constituents comprising 100% of the essential oil were characterized by gas chromatography/mass spectrometry (GC-MS) techniques. The major compounds in the essential oil were (+)-Sabinene (34.73%), Cis-α-bergamotene (13.15%), Z-α-trans-bergamotol (5.28%), β-mycrene (4.98%), and 1,3,6-octatriene, 3,7-dimethyl-(4.71%). Antibacterial activities of Melicope pteleifolia essential oil were investigated against Gram-positive and-negative bacteria. Results showed significant activities against Streptococcus pyogenes and Escherichia coli using an agar well diffusion method. The application of this essential oil in preventing and eliminating bacteria could be useful in fields as medicine and cosmetics.

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The Optimization of Extraction Process, Antioxidant, Whitening and Antibacterial Effects of Fengdan Peony Flavonoids

Molecules ◽

10.3390/molecules27020506 ◽

2022 ◽

Vol 27 (2) ◽

pp. 506

Author(s):

Jie Lu ◽

Zhiqiang Huang ◽

Yusheng Liu ◽

Huimin Wang ◽

Min Qiu ◽

...

Keyword(s):

Free Radical ◽

Functional Food ◽

Biological Activities ◽

Extraction Process ◽

Dry Mass ◽

Raw Material ◽

Seed Meal ◽

Total Flavonoids ◽

Minimal Inhibitory Concentrations ◽

Great Possibility

Flavonoids have important biological activities, such as anti-inflammatory, antibacterial, antioxidant and whitening, which is a potential functional food raw material. However, the biological activity of Fengdan peony flavonoid is not particularly clear. Therefore, in this study, the peony flavonoid was extracted from Fengdan peony seed meal, and the antioxidant, antibacterial and whitening activities of the peony flavonoid were explored. The optimal extraction conditions were methanol concentration of 90%, solid-to-liquid ratio of 1:35 g:mL, temperature of 55 °C and time of 80 min; under these conditions, the yield of Fengdan peony flavonoid could reach 1.205 ± 0.019% (the ratio of the dry mass of rutin to the dry mass of peony seed meal). The clearance of Fengdan peony total flavonoids to 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical, hydroxyl radical and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical could reach 75%, 70% and 97%, respectively. Fengdan peony flavonoid could inhibit the growth of the Gram-positive bacteria. The minimal inhibitory concentrations (MICs) of Fengdan peony flavonoid on S. aureus, B. anthracis, B. subtilis and C. perfringens were 0.0293 mg/mL, 0.1172 mg/mL, 0.2344 mg/mL and 7.500 mg/mL, respectively. The inhibition rate of Fengdan peony flavonoid on tyrosinase was 8.53–81.08%. This study intensely illustrated that the antioxidant, whitening and antibacterial activity of Fengdan peony total flavonoids were significant. Fengdan peony total flavonoids have a great possibility of being used as functional food materials.

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Chemical Composition, Enantiomeric Distribution, and Biological Activities of Rhododendron anthopogon Leaf Essential Oil from Nepal

Natural Product Communications ◽

10.1177/1934578x1601101230 ◽

2016 ◽

Vol 11 (12) ◽

pp. 1934578X1601101 ◽

Cited By ~ 2

Author(s):

Noura S. Dosoky ◽

Prabodh Satyal ◽

Suraj Pokharel ◽

William N. Setzer

Keyword(s):

Gas Chromatography ◽

Chemical Composition ◽

Essential Oil ◽

Biological Activities ◽

Gas Chromatography Mass Spectrometry ◽

Volatile Components ◽

Cytotoxic Activities ◽

Blood Disorders ◽

Leaf Essential Oil ◽

Microbroth Dilution

Rhododendron anthopogon D. Don., a small compact Himalayan shrub growing in Nepal, is a known medicinal plant used to treat sore throat, colds, blood disorders, bone disease, potato allergies, and vomiting, and to relieve liver disorders, headaches and back pain. The present study investigated the chemical composition and bioactivities of the leaf essential oil from R. anthopogon from Dhankuta, Nepal. The essential oil from leaves was obtained by hydrodistillation and a detailed chemical analysis was conducted by gas chromatography – mass spectrometry (GC-MS). The enantiomeric distribution of monoterpenoid components was determined using chiral gas chromatography and represents the first chiral examination of R. anthopogon essential oil. The essential oil was screened for antimicrobial activity using the microbroth dilution test, and for cytotoxic activity against MCF-7, MDA-MB-231, and 5637 using the MTT assay. A total of 70 volatile components were identified from the essential oil. The major components were α-pinene (21.5%), δ-cadinene (13.8%), β-pinene (9.5%), limonene (5.9%), δ-amorphene (4.6%), α-muurolene (4.5%), and ( E)-caryophyllene (3.2%) with other minor constituents (< 3%). The essential oil showed marginal antibacterial and cytotoxic activities, but no antifungal effects.

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