Can indolebutyric and fulvic acids induce adventitious rhizogenesis on mini-cuttings from Brazilian native tibouchinas?

The use of Brazilian native species for ornamental purposes is a promising alternative for local floriculture. Seeking to contribute with new information in this context, the present study aimed to evaluate the rooting performance of mini-cuttings from three Tibouchina species (T. aff. fothergillae, T. heteromalla and T. moricandiana var. vinacea) as affected by the use of indolebutyric acid (IBA) and fulvic acid (FA). Mini-cuttings with 5 cm in length were prepared with plant material from clonal mini-hedges and submitted to different treatments as follows: control treatment (T1); 2,000 mg L-1 IBA (T2); 2,000 mg L-1 FA (T3) and 2,000 mg L-1 IBA + 2,000 mg L-1 FA (T4). Planting was carried out in plastic containers filled with vermiculite, and, after 26 days under greenhouse conditions, the following variables were evaluated: mini-cuttings rooting percentage (RP), roots number (RN), roots length (RL), initial leaves maintenance (ILM) and sprouting (SP). The experiment was conducted under a completely randomized design in a 3 x 4 factorial scheme, with four replications and 20 minicuttings per plot. Rooting percentages were higher than 90% in all three species, regardless of IBA or FA treatments. RP, ILM and SP did not show statistically significant interaction between the treatments. For RN, T2 and T4 promoted the best results on T. aff. fothergillae (12.62 and 14.92, respectively) and T2 resulted in maximum values for T. heteromalla (15.65). For RL, T2 and T4 were statistically superior on T. heteromalla (9.52 and 8.20 cm, respectively). The use of IBA and FA is dispensable for rhizogenesis induction on mini-cuttings from the studied species

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PROPAGAÇÃO DE Cestrum corymbosum POR SEMENTES E ESTAQUIA

FLORESTA ◽

10.5380/rf.v43i1.25012 ◽

2013 ◽

Vol 43 (1) ◽

pp. 137 ◽

Cited By ~ 1

Author(s):

Christopher Thomas Blum ◽

Valdeci Constantino ◽

Karen Koch Fernandes de Souza ◽

Antônio Carlos Nogueira

Keyword(s):

Significant Influence ◽

Vegetative Propagation ◽

Native Species ◽

Indolebutyric Acid ◽

Randomized Design ◽

High Germination ◽

Completely Randomized Design ◽

Propagation Experiment ◽

Germination And Growth ◽

Very High

Cestrum corymbosum é um arbusto ornamental nativo do Brasil. A pesquisa visou estudar sua propagação sexuada e vegetativa, avaliando a germinação e o crescimento e testando o efeito do tamanho das estacas e do ácido indolbutírico (AIB) no seu enraizamento e desenvolvimento. Para avaliar a germinação, foram utilizadas seis repetições de 100 sementes. Em seguida, 200 plântulas foram transferidas para embalagens individuais mantidas em canteiro. O experimento de propagação vegetativa, com delineamento inteiramente casualizado em arranjo fatorial 3 x 2, constituiu-se na combinação de três concentrações de AIB (0, 1000 e 2000 mg.L-1) e dois tamanhos de estacas (8 e 15 cm). As estacas foram plantadas em substrato comercial Plantmax HA®. No experimento de propagação sexuada, verificou-se elevada germinação (92%), assim como alta taxa de sobrevivência após repicagem para recipientes individuais (99,5%). Para o experimento de propagação vegetativa, foi detectada influência significativa do tamanho das estacas em relação à sobrevivência, enraizamento (P < 0,01) e número de folhas (P < 0,05). Estacas de 8 cm apresentaram melhor sobrevivência (93,3%) e enraizamento (91,3%). A aplicação de concentrações de AIB não resultou em diferenças significativas (P < 0,01), demonstrando não ser necessária para o enraizamento dessa espécie.Palavras-chave: Germinação; enraizamento; ácido indolbutírico; espécie nativa; Solanaceae.AbstractPropagation of Cestrum corymbosum by seeds and cuttings. Cestrum corymbosum is an ornamental bush native from Brazil. This research aimed to study the sexual and vegetative propagation of this species, evaluating germination and growth of the seedlings, and testing the influence of indolebutyric acid (IBA) and cuttings size on its rooting and development. In order to evaluate germination, six repetitions of 100 seeds were considered. After that, 200 seedlings have been transplanted to individual packages kept in a bed. The vegetative propagation experiment has been arranged in a 3 x 2 factorial completely randomized design that comprised the combination of three IBA concentrations (0, 1000 and 2000 mg.L-1) for two cuttings sizes (8 and 15 cm). The cuttings were planted in commercial substrate Plantmax HA®. A high germination (92%) has been verified. The survival after transplantation was very high (99.5%). It was detected statistically significant influence of the cuttings size on plants survival, rooting (P<0.01) and leaves number (P<0.05). The cuttings of 8 cm presented better survival (93.3%) and rooting (91.3%) rates. The treatment with IBA in distinct concentrations did not produce results with significant differences (P<0.01).Keywords: Germination; rooting; indolebutyric acid; native species; Solanaceae.

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Mortality Assessment of Botanical Oils on Anastrepha fraterculus (Wiedemann, 1830) Applied in Fruits Under Laboratory Conditions

Journal of Agricultural Science ◽

10.5539/jas.v11n8p287 ◽

2019 ◽

Vol 11 (8) ◽

pp. 287

Author(s):

Dahise Brilinger ◽

Cleiton L. Wille ◽

Joatan M. da Rosa ◽

Cláudio R. Franco ◽

Mari Inês C. Boff

Keyword(s):

Second Generation ◽

Fruit Fly ◽

Adult Mortality ◽

Control Treatment ◽

Carapa Guianensis ◽

Anastrepha Fraterculus ◽

Randomized Design ◽

Completely Randomized Design ◽

Plastic Containers ◽

Apple Fruits

The objective of this study was to evaluate the effect of botanical oils on adult mortality and oviposition repellency of Anastrepha fraterculus in peach, apple and red cattley guava fruits. The fruits were treated by the dip method for 15 seconds with botanical oils of Ruta graveolens (0.05%), Cymbopogon citratus (1%), Cymbopogon winterianus (10%), Carapa guianensis (25%) and distilled water was used as a control. The experiments were carried out in a completely randomized design with twenty replicates. After being treated, the fruits were dried at room temperature, placed in separate plastic containers (750 mL) and transferred to a room at 25±2 °C. Each fruit was exposed to two fruit fly couples for 48 hours. After this period, the amount of dead insects was recorded. On apple fruits kept in containers with sterilized vermiculite, the effect of the treatments on A. fraterculus biology was evaluated for 36 days, and data were collected on the amount of pupae in the second generation. C. winterianus oil presented mortality rates of 100 and 80% of adults in peach and apple fruits, respectively. C. citratus oil caused a significantly different mortality rate compared to the control in peach fruits, whereas C. guianensis oil caused A. fraterculus mortality in red cattley guava and apple fruits. The mortality of A. fraterculus in fruits treated with R. graveolens oil did not differ from the control treatment. The botanical oils of C. winterianus and C. guianensis protected apple fruits by preventing A. fraterculus proliferation, and there was a significant reduction of the amount of pupae in the second generation. Botanic oils presented potential for suppression of fruit flies; however, further studies are necessary to test and make them feasible under field conditions.

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MICRONUTRIENT CONTENTS IN THE PROFILE OF A RECOVERED SOIL WITH SEWAGE SLUDGE AND NATIVE SPECIES OF ATLANTIC FOREST

FLORESTA ◽

10.5380/rf.v49i3.59351 ◽

2019 ◽

Vol 49 (3) ◽

pp. 485

Author(s):

Lívia Mara Lima Goulart ◽

Marianne Fidalgo de Faria ◽

Grasiela Spada ◽

Thiago Tássio de Souza Silva ◽

Iraê Amaral Guerrini

Keyword(s):

Sewage Sludge ◽

Atlantic Forest ◽

Soil Profile ◽

Native Species ◽

Control Treatment ◽

Mineral Fertilization ◽

Promising Alternative ◽

Final Destination ◽

Potassium Supplementation

The use of sewage sludge in agriculture and recovery of degraded areas has been shown as a promising alternative for its final destination. Studies on micronutrient levels after sludge application are necessary to avoid soil contamination at toxic levels. The objective of this work was to verify the micronutrient contents in the soil profile and pH, up to one-meter-deep, nine years after the application of sewage sludge and planting of native species of the Atlantic Forest. The experiment was implemented in a degraded Quartzeneic Neosol and conducted in randomized blocks with four replicates and eight treatments, consisting of six doses of sewage sludge (0, 2.5, 5, 10, 15 and 20 Mg ha-1, with supplementation of potassium due to low concentration in the residue), besides the control treatment, mineral fertilization and only potassium supplementation. After nine years, the contents of all micronutrients evaluated presented a significant response to the application of the treatments, and the application of sewage sludge provided an increase in their contents. Soil pH remained stable at sites receiving mineral fertilization and potassium supplementation. Only manganese and zinc showed mobility in the soil profile. The application of sewage sludge in degraded soil increases the micronutrient content and decreases its movement in the soil profile, and the application of the maximum dose of the residue does not provide toxic levels of these elements in the soil in the long term.

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Effect of Ethephon and Packaging Practices On Ripening of Mango cv. Maldah

SAARC Journal of Agriculture ◽

10.3329/sja.v19i1.54786 ◽

2021 ◽

Vol 19 (1) ◽

pp. 155-163

Author(s):

S Sapkota ◽

D Kc ◽

H Giri ◽

M Saud ◽

M Basnet ◽

...

Keyword(s):

Chemical Properties ◽

Control Treatment ◽

Quality Performance ◽

Plastic Packaging ◽

Randomized Design ◽

Acid Ratio ◽

Ethephon Treatment ◽

Completely Randomized Design ◽

And Control ◽

Juice Content

The present research was conducted on two factor Completely Randomized Design (CRD) with eight treatments and three replications. A set of experiments were carried out to evaluate the effect of postharvest ethephon treatment and packaging on ripening of mango cv. Maldah. The treatments consisted of ripening agent i.e., ethephon and control treatment under different packaging condition i.e., fiber with hole, fiber without hole, plastic with hole and plastic without hole. The result revealed that different packaging condition and ripening agents influenced the ripening behavior of mango. The highest TSS (15.26), sugar-acid ratio (23.66) and juice content (126.05) were recorded with fiber (without hole) and the lowest TSS (12.60), sugar-acid ratio (9.01) and juice content (116.05) with plastic (without hole). The highest TA (1.44) was recorded with plastic (without hole) and the lowest (0.66) with fiber (without hole). Similarly, the highest BT (2.83) was recorded with fiber (with hole) and the lowest (1.66) with plastic (without hole). Firmness, sweetness, TSS and juice content were the highest with the interaction effect of fiber bag (without hole) and ethephon treatment. In conclusion, mango fruits with ethephon treatment packed in fiber bag (without hole) enhances quality and ripening of mango whereas under controlled condition and without hole plastic packaging mangoes had low quality performance in terms of physio-chemical properties. SAARC J. Agric., 19(1): 155-163 (2021)

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Effect of biofertilizer obtained by anaerobic digestion of cassava effluent on the development of crambe plants

Revista Brasileira de Engenharia Agrícola e Ambiental ◽

10.1590/1807-1929/agriambi.v21n10p681-685 ◽

2017 ◽

Vol 21 (10) ◽

pp. 681-685 ◽

Cited By ~ 3

Author(s):

Andressa C. Neves ◽

Camila N. Bergamini ◽

Rafaela de O. Leonardo ◽

Manoel P. Gonçalves ◽

Dilcemara C. Zenatti ◽

...

Keyword(s):

Anaerobic Digestion ◽

Plant Development ◽

Root Biomass ◽

Oil Content ◽

Final Height ◽

Control Treatment ◽

Randomized Design ◽

Completely Randomized Design ◽

Shoot And Root Biomass ◽

Number Of Branches

ABSTRACT This study aimed to evaluate the effect of applying increasing doses of biofertilizer obtained by the anaerobic digestion of cassava effluent on the development of crambe plants. The experiment was conducted in a protected environment at the Federal University of Paraná (UFPR), Palotina Sector, between April and August 2015. A completely randomized design was used, and five different treatments with the following doses were applied in five replicates: 0, 40, 80, 120, and 160 kg ha-1 of K2O. The following parameters related to plant development were evaluated: final height, stem diameter, number of branches, dry shoot and root biomass, mass of the grains, and oil content. The 160 kg K2O ha-1 dose was found to have the best influence on the plant development, because all the measured parameters reached their highest values at this dose, except for oil content, which attained the highest percentage in the case of the control treatment (0 kg ha-1 of K2O). This study proved that the biofertilizer obtained by anaerobic digestion of cassava effluent can be used as an alternative to regular fertilizers in cultivating crambe.

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Methionine plus cystine requirements for the maintenance and efficiency of utilization with tambaqui of different body weights

Ciência e Agrotecnologia ◽

10.1590/1413-7054202044014920 ◽

2020 ◽

Vol 44 ◽

Author(s):

Neliane Galvão Porto ◽

Felipe Barbosa Ribeiro ◽

Jefferson Costa de Siqueira ◽

Marcos Antonio Delmondes Bomfim ◽

Rafael Silva Marchão ◽

...

Keyword(s):

Amino Acid ◽

Control Treatment ◽

Native Fish ◽

Amino Acid Requirement ◽

Treatment Groups ◽

Protein Retention ◽

Lack Of Information ◽

Randomized Design ◽

Body Weights ◽

Completely Randomized Design

ABSTRACT Methionine is an essential amino acid, and generally, the first limiting one in the diets of tambaqui, the main native fish produced in continental aquaculture in South America. However, there is a lack of information on their amino acid requirement, especially for maintenance and efficiency of utilization. The present study aimed to determine the requirement of [methionine plus cystine] for the maintenance and efficiency of utilization using tambaqui of different body weights. Two experiments were carried out using 250 tambaqui with weights of 100 and 300 g. They were distributed in a completely randomized design with four treatment groups (0.264, 0.396, 0.528, and 0.660% of digestible [Met + Cys]), five replications. The control treatment (CT) was performed with the addition of methionine at the first level (0.264%) until the second level was reached (0.396%). The maintenance requirement was determined by the regression analysis between the consumption of [Met + Cys] and protein retention equal to zero. The efficiency was determined using the value of the slope coefficient of the line obtained by the linear regression. The protein and methionine retention values of tambaqui weighing 100 to 300 g were compared by the parallelism test, and no difference was observed between the parameters of the equations indicating the need for only one equation to describe the responses to body weights. The requirement of digestible [Met + Cys] for the maintenance of tambaqui with weights from 100 to 300 g was 60.47 mg kg-0.7 day-1 and the efficiency of utilization of 42%.

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The Affection of Fine Bubbles (FBs) Application on Growth Siamese Catfish (Pangasianodon hypophthalmus) in Aquaponic System

Asian Journal of Fisheries and Aquatic Research ◽

10.9734/ajfar/2020/v7i430127 ◽

2020 ◽

pp. 30-36

Author(s):

Rahmad Afdillah ◽

Yuli Andriani ◽

Zahidah Hasan ◽

. Rosidah ◽

. Iskandar

Keyword(s):

Water Quality ◽

Specific Growth ◽

Good Condition ◽

Quality Parameters ◽

Control Treatment ◽

Effective Pressure ◽

Growth And Survival ◽

Pangasianodon Hypophthalmus ◽

Randomized Design ◽

Completely Randomized Design

This research aims was to determine the effective pressure on fine bubbles technology on the growth of Siamese catfish fry in the aquaponic system. This study uses a completely randomized design with four treatments and three replications. Treatment A (Control), Treatment B (FBs at a pressure of 4,5 atm), Treatment C (FBs at a pressure of 5 atm and treatment D (FBs at a pressure of 5,5 atm). The parameters observed were Specific Growth Rate, Survival Rate and water quality (Temperature, pH, DO, Ammonia) Data were analyzed using variance with the F test at a 95% confidence level. The best treatment for catfish growth was treatment D (5,5 atm pressure), which gave the highest SGR value of 7,24% and the highest SR value of 100%. The value of water quality parameters were in good condition for the growth and survival of catfish.

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Pengaruh penambahan serbuk jagung pada komposisi media tanam terhadap pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus)

BIOMA Jurnal Ilmiah Biologi ◽

10.26877/bioma.v10i2.7429 ◽

2021 ◽

Vol 10 (2) ◽

pp. 158-170

Author(s):

Muhaeming Muhaeming ◽

Jamilah Jamilah ◽

Zulkarnaim Zulkarnaim

Keyword(s):

Experimental Study ◽

Analysis Of Variance ◽

Pleurotus Ostreatus ◽

Medium Composition ◽

Oyster Mushroom ◽

Control Treatment ◽

Mycelium Growth ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design

Penambahan nutrisi perlu dilakukan untuk meningkatkan produktivitas pertumbuhan jamur tiram putih (Pleurotus ostreatus). Salah satu bahan yang dapat digunakan adalah serbuk jagung. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan serbuk jagung pada komposisi media tanam terhadap pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus) dan mengetahui konsentrasi serbuk jagung yang paling baik digunakan pada pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus). Penelitian ini mengunakan Rancangan Acak Lengkap (RAL) 4 perlakuan dan 3 ulangan sehingga diperoleh 12 satuan percobaan; P0 (perlakuan kontrol), P1 (25%), P2 (50%), dan P3 (100%). Variabel yang diukur adalah persentase pertumbuhan miselium yang diamati pada hari ke-6, 12 dan 18 dan waktu penyebaran miselium Hari Setelah Inokulasi (HSI). Data hasil penelitian dianalisis menggunakan analysis of variance (ANOVA) dengan uji lanjut menggunakan uji Beda Nyata Terkecil (BNT). Hasil penelitian menunjukkan bahwa ada pengaruh pada pertumbuhan miselium bibit F1 jamur tiram putih (Pleurotus ostreatus) dengan penambahan serbuk jagung pada komposisi media tanam. Selain Itu, konsentrasi serbuk jagung yang memberikan hasil yang paling baik terhadap pertumbuhan miselium adalah P2 dengan konsentrasi 50%. Kata kunci : miselium F1; Pleurotus ostreatus; serbuk jagung ABSTRACTAdditional nutrition needs to be done to increase the productivity of oyster mushroom (Pleurotus ostreatus) growth. One of the materials that can be used is corn powder. This study aimed to determine the effect of corn powder on the planting medium composition towards mycelium growth of oyster mushroom F1 seeds (Pleurotus ostreatus) and to find the best concentration of corn powder to grow mycelium seeds F1 oyster mushroom (Pleurotus ostreatus). This research was an experimental study using a Completely Randomized Design (CRD) with 4 treatments namely; P0 (control treatment), P1 (25%), P2 (50%), and P3 (100%), and 3 repetitions so that obtained 12 experimental units. The variables measured were the mycelium growth percentage on days 6, 12, and 18, and the mycelium widespread time after the day of inoculation. The data were analyzed using ANOVA then continued on the Least Significant Difference (LSD) test. The results showed that the addition of corn powder on oyster mushroom growing media affected mycelium growth of the oyster mushroom F1 seeds (Pleurotus ostreatus). It also found that the P2 with a 50% concentration of corn powder gave the widest mycelium growth. Keywords: corn powder; mycelium; Pleurotus ostreatus

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APPLICATION OF BENZYLADENINE (BA) IN THE FORM OF LANOLIN PASTE ON FLOWER-STALK BUDS SUCCESSFULLY INDUCED RE-BLOOMING OF HYBRID Phalaenopsis

Jurnal Agrotek Tropika ◽

10.23960/jat.v8i2.3915 ◽

2020 ◽

Vol 8 (2) ◽

pp. 383

Author(s):

Mukhaila Iryani ◽

Yusnita Yusnita ◽

Dwi Hapsoro ◽

Kukuh Setiawan ◽

Agus Karyanto

Keyword(s):

Plant Growth ◽

The Other ◽

Control Treatment ◽

Bud Break ◽

Flower Stalk ◽

Moth Orchid ◽

Randomized Design ◽

Time Period ◽

Long Time ◽

Completely Randomized Design

Hybrid moth orchid (genus Phalaenopsis) is one of the most popular ornamentals in Indonesia. It has beautiful and long-lasting flowers, but cultivating this orchid is still become a challenging issues due to the need of specific condition to grow and long time period to re-blooming. Plant growth regulators (PGR) (i.e. Benzyladenine (BA)) has been widely documented as a flower-inducing substance in several orchids. However, the optimal concentration and its mechanism in inducing flower-stalk bud and re-blooming is still unclear. This research aimed to study the effects of BA application in the form of lanolin paste on hybrid Phalaenopsis flower-stalk buds. We conducted this study using completely randomized design with four replications at the greenhouse laboratory Faculty of Agriculture University of Lampung on August to December 2018. We divided the orchid into 5 group of BA concentration (0, 1000, 1500, 3000, or 6000 ppm). The sheath of the fourth or fifth buds of the flowers were carefully opened, then it smeared with BA. The percentage of bud break into flower spike or keiki, length of shoots or spike and number of open flowers were recorded until 10 weeks of observation. The results showed that, neither of the buds under the control treatment (without BA), 1000 ppm nor 1500 ppm BA broke and grew into keiki or spike. On the other hand, application of BA at 3000 ppm or 6000 ppm successfully induced 100% flower spikes on the buds treated. No keiki was formed in all buds treated. In addition, treatment of the buds with 6000 ppm BA produced longer flower spikes as well as more open flowers. We conclude that the application of BA (minimal 3000 ppm) successfully induced flower spike of hybrid Phalaenopsis.

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Cryopreservation in liquid nitrogen of Brazilian orchid seeds Miltonia flavescens Lindl.

Revista Agro mbiente On-line ◽

10.18227/1982-8470ragro.v14i0.6388 ◽

2020 ◽

Vol 14 ◽

Author(s):

Jean Carlo Baudraz de Paula ◽

Walter Aparecido Ribeiro Júnior ◽

Gabriel Danilo Shimizu ◽

Gabriel Barraca Men ◽

Ricardo Tadeu De Faria

Keyword(s):

Low Temperature ◽

Water Content ◽

Liquid Nitrogen ◽

Biological Material ◽

Control Treatment ◽

Preservation Methods ◽

Lower Performance ◽

Randomized Design ◽

Completely Randomized Design ◽

Protocorm Formation

Miltonia flavescens is a species vulnerable to extinction, which justifies research on preservation methods. Cryopreservation in liquid nitrogen (LN) consists of maintaining biological material at a low-temperature (-196 °C). Thus, the aim of the experiment is to evaluate the influence of different cryoprotective solutions on cryopreservation in LN of the Brazilian orchid Miltonia flavescens seeds. The experiment was conducted in a completely randomized design (CRD), with eight treatments and six replications. The treatments were composed as follows: control; immersion in LN, no cryoprotectant adding; and immersion in LN, with the addition of cryoprotectants: sucrose 0,4 mol L-1; glycerol 2 mol L-1; protection by vitrification in solution (PVS)1; PVS2; PVS2 + 1% phloroglucinol, and PVS3. Except for the control treatment, which was kept in a freezer (10±2 °C), the others remained frozen for 15 days. After this period, the viability of the seeds was evaluated. These seeds were sown and, 30 days after germination, then the frequency of protocorm formation was verified. Before the cryopreservation, the seeds showed 75% viability and 9.5% water content. After cryopreservation, the seeds varied between 67 to 75% viability. However, treatment with glycerol 2 mol L-1 exhibited lower performance than the others (58%). The control treatment showed a higher percentage of protocorm formation (71%) followed by treatments PVS1 (63%), PVS2 (64%), and PVS2PHLO (66%). For the purpose of preserving Miltonia flavescens seeds in liquid nitrogen for a prolonged period, the treatments PVS1, PVS2, and PVS2PHLO proved to be viable and promising alternatives.

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