scholarly journals The Antioxidant Properties of Black Garlic Aqueous Extract in Vero Cell Line

2020 ◽  
Vol 3 ◽  
pp. 85-89
Author(s):  
Amna Mohamed Abdulhafith Marie ◽  
Nastiti Wijayanti
Keyword(s):  
Free Radical ◽  
Aqueous Extract ◽  
Antioxidant Properties ◽  
Vero Cells ◽  
Oxygen Free Radical ◽  
The Body ◽  
Cytotoxicity Test ◽  
Griess Reagent ◽  
Level Measurement ◽  
Unstable Molecules

Oxidative stress has been defined as harmful because oxygen free radical attack biological molecules such as lipids, proteins and DNA. Antioxidants are substances that can prevent or slow damage to cells caused by free radicals, unstable molecules that the body produces as a reaction to environmental and other pressures. Black garlic (BG) is one of the natural resources that has been reported to have antioxidant properties. The aims of this research was to study the potential and effect of BG as antioxidant in LPS induced Vero cells. The methods of this research were used the extraction of BG by maceration method using aqueous solvents, antioxidant activity tested using DPPH assay, cytotoxicity test with WST-1 assay on 96-well plate, and NO level measurement using Griess reagent, after cells induced with LPS. The result showed that in DPPH assays BG showed ability for scavenging free radical activities. The BG aqueous extract did not show cytotoxicity on Vero cells until the concentration of 750µg/ml and NO levels decreased in cells treated with a concentration of 50 µg/mL after LPS induction. This study concluded that the administration of aqueous extract of Black Garlic has the potential as an antioxidant.

Strong antihemolytic and antioxidant properties of aqueous extract from Algerian Hammada elegans (Bge.) Botsch (Chenopodiaceae)

2021 ◽  
Vol 17 ◽  
Author(s):  
Brahim Asseli ◽  
Reguia Mahfoudi ◽  
Amar Djeridane ◽  
Mohamed Yousfi
Keyword(s):  
Free Radical ◽  
Aqueous Extract ◽  
Radical Scavenging Activity ◽  
Condensed Tannin ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Dependent Manner ◽  
Vitro Assay

Background: Research on medicinal plant antioxidants has emerged as a potential therapeutic to prevent free radical generated damage in the human body. Hammada elegans Botsch (popularly known as “Ajram”) is a xerophytic plant widely found in Laghouat region, but there are only a few reports about the biological or chemical properties of these species. Hence, the aim of this study is to investigate the antioxidant and the antihemolytic activities of hexanic, acetonic, methanolic and aqueous extracts of aerial parts of Algerian Hammada elegans Botsch by employing different in vitro assay systems. Methods: The total phenolic content, the flavonoid content and the condensed tannin amount were analyzed using Folin-Ciocalteu, aluminum chloride and vanillin assays, respectively. The in vitro antioxidant capacity of extracts was assessed by CUPRAC, iron chelating, ABTS•+and antihemolytic assays, and was expressed as EC50 values. Results: Among the analyzed extracts, the aqueous extract had the highest phenolic, flavonoid and tannin contents. Also, this extract displayed the highest antioxidant capacities compared to the other extracts and standards. Its EC50 value for ABTS radical-scavenging activity was 0.265 ± 0.003 mg/L. Moreover, this extract showed high iron (II) chelating ability (EC50 = 0.958 ± 0.001 mg/L), and good antioxidant activity in the cupric ion reducing activity (CUPRAC) in a concentration dependent manner (EC50 were 0.709 ± 0.002 mg/L). Additionally, this extract had the best antihemolytic activity against AAPH-induced hemolysis (EC50=0.090 ± 0.004 mg/L). Conclusion: Our study revealed that the aqueous extract of Hammada elegans Botsch, is a potential source of antioxidants which possess a high protective effect of membrane against free radical.

Amlodipine Decreases Iron Uptake and Oxygen Free Radical Production in the Heart of Chronically Iron Overloaded Mice

2002 ◽  
Vol 3 (4) ◽  
pp. 189-197 ◽  
Author(s):  
Sarah Crowe ◽  
Wally J. Bartfay
Keyword(s):  
Free Radical ◽  
Iron Metabolism ◽  
Iron Uptake ◽  
Hereditary Hemochromatosis ◽  
Antioxidant Properties ◽  
Oxygen Free Radical ◽  
Cardiac Complications ◽  
Channel Blockers ◽  
Free Radical Production ◽  
Radical Production

Hereditary hemochromatosis is a disorder of iron metabolism, which is currently the most prevalent autosomal recessive disorder in the world, with an expression of the homozygous form occurring in approximately 1 in 200 individuals of European descent. Approximately one third of patients with hemochromatosis die of iron-induced cardiac complications. Although the exact mechanism is not known, it is believed that the toxicity of excess iron in biological systems is due to its ability to catalyze the generation of harmful reactive oxygen free radical species (ROS), which can damage proteins, lipids, and DNA. There is preliminary evidence to suggest that nontransferrinbound iron uptake in the myocardium may occur through voltage-dependent L-type calcium channels, and that calcium channel blockers (CCBs) may possess antioxidant properties. Accordingly, the authors hypothesized that the administration of amlodipine besylate would (1) decrease iron uptake in the myocardium and (2) decrease oxygen free radical production as measured by cytotoxic aldehyde–derived peroxidation products in a murine model of iron overload cardiomyopathy. The findings show that the CCB amlodipine is partially effective in limiting iron uptake in the heart and significantly inhibits the production of ROS in chronically iron-loaded mice. These are important preliminary findings because they suggest that CCBs may have significance in the clinical management of genetic disorders of iron metabolism.

scholarly journals Hibiscus sabdariffa extract as anti-aging supplement through its antioxidant and anti-obesity activities

2020 ◽  
Vol 7 (1) ◽  
pp. 3572-3578
Author(s):  
Zuliana Muhamad Ridzwan Krishnamurthy ◽  
Nurzalina Abdul Karim Khan ◽  
Insathe Mohd Ali ◽  
Mohamad Dayoob ◽  
Shahad Shakho Hussein
Keyword(s):  
Aqueous Extract ◽  
Pancreatic Lipase ◽  
Healthy Aging ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
The Body ◽  
Hibiscus Sabdariffa ◽  
Ethanolic Extracts ◽  
Alpha Glucosidase

Introduction: Imbalance between total energy intake and expenditure causes accumulation of excess fat and sugar in the body which leads to development of diabetes mellitus type II, obesity, and metabolic syndrome. These harmful diseases accelerate aging and cause fatal metabolic disorders as people age. Inhibition of pancreatic lipase, and alpha glucosidase digestive enzymes is a step that can reduce excess fat and sugar from the body, which is an essential component of healthy aging. Methodology: In this study, aqueous and 50% ethanolic extracts of Hibiscus sabdariffa were investigated for their inhibitory activities on pancreatic lipase and alpha-glucosidase, in addition to their antioxidant activities (using UV-vis spectrophotometer). Results: Both extracts displayed antioxidant properties, indicated by IC50 of 5166.80 mg/mL for H. sabdariffa aqueous extract and 2809.10 mg/mL for H. sabdariffa 50% ethanolic extract. The extracts also suppressed the activities of pancreatic lipase and alpha-glucosidase enzymes, which suggests possible antiobesity and anti-diabetic activities. H. sabdariffa aqueous extract inhibited pancreatic lipase activity with IC50 of 167.5+/-12.7 mg/mL, whereas H. sabdariffa 50% ethanolic extract inhibited the enzyme with an IC50 of 790.65+/-16.02 mg/mL. Both H. sabdariffa aqueous and ethanolic extracts also successfully inhibited alpha-glucosidase enzyme activity with IC50 949.88 +/-10.83 mg/mL, and 378.33 +/-4.20 mg/mL, respectively. Conclusion: Taken together, the outcome of the investigations offers the possibility of the extracts as an anti-obesity, anti-diabetic, anti-metabolic and anti-aging agent, which can be developed into supplements for adults to prevent the occurrence of these prevalent diseases and delay the onset and effects of aging.  

scholarly journals In vitro oocysticidal sporulation inhibition of Eimeria tenella and antioxidant efficacy of ethanolic and aqueous extracts of Conyza aegyptiaca

2021 ◽  
Vol 6 (1) ◽  
pp. 30-40
Author(s):  
Emmanuel Tana Toah ◽  
◽  
Vincent Khan Payne1, ◽  
Yamssi Cedric ◽  
Noumedem Anangmo Christelle Nadia ◽  
...  
Keyword(s):  
Free Radical ◽  
Aqueous Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Hot Water ◽  
Phytochemical Analysis ◽  
Aqueous Extracts

Avian coccidiosis is probably one of the most expensive parasitic diseases with major economic impact on poultry industries worldwide. The purpose of this study was to evaluate the ethanolic and aqueous extracts of Conyza aegyptiaca in terms of phytonutrients, in vitro oocysts sporulation inhibition and antioxidant properties. The extraction process of plant leaf powder (100 g) pulverized using a clean manual grinder was carried out in ethanol and hot water and the yields were calculated as a percentage ratio of extract mass on plant powder mass after solvent evaporation. Phytochemical analysis procedures were performed to determine the presence of phytonutrients. The in vitro oocysticidal sporulation inhibition was determined at five different concentrations (0.25; 0.5; 1; 2 and 4 mg/ml) of each extract in petri dishes each containing 3000 unsporulated oocysts and examined after 24 and 48 hours under a microscope. In vitro antioxidant capacity of extracts was estimated using different assays. Quantitative aqueous extract (11.72%) was higher than ethanolic extract (4.34%). In terms of qualitative yields, ethanolic extract revealed higher phytonutrients investigated (100%) than aqueous extract (42.86%). The sporulation inhibition of ethanolic extract was generally higher than the aqueous extract after 24 and 48 h and varied according to the different tested concentrations. In all the antioxidant assays, ethanolic extract exhibited significant free radical scavenging activity with inhibitory concentration (IC50=26.10±1.09) close to that of ascorbic acid at the probability level of 5% error (p<0.05). The ethanolic extract with higher free radical scavenging activities and ferric reducing effect also showed significant higher content of both phenols (127.01±3.99 mgGAE/g) and flavonoids (108.66±3.49 mgCE/g) than aqueous extracts, suggesting correlation between phenolic content and antioxidant activity. Data from this study could be used for developing bioactive elements for natural anticoccidials and antioxidants of health promoting activities

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN BUAS-BUAS (Premna serratifolia L.) ASAL KABUPATEN MELAWI PROVINSI KALIMANTAN BARAT DENGAN METODE DPPH

2020 ◽  
Vol 3 (2) ◽  
pp. 405-412
Author(s):  
Weni Puspita ◽  
◽  
Dina Yuspita Sari ◽  
Ika Ristia Rahman ◽  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Cell Damage ◽  
Antioxidant Properties ◽  
Free Radical Oxidation ◽  
The Body ◽  
Radical Oxidation ◽  
Ic50 Values ◽  
Ethanol Extracts

Buas (Premna serratifolia L.) contains secondary metabolites of flavonoids, saponins, tannins and triterpenoids/steroids, where flavonoids are known to have antioxidant properties and counteract free radicals. Antioxidants are compounds that can fight toxic hazards and reduce cell damage to the body caused by free radical oxidation. The purpose of this study was to determine the antioxidant activity of ethanol extracts of buas-buas leaves was carried out by DPPH (2,2-diphenyl-1-picrylhydrazyl), using UV-Vis spectrophotometry at wavelength 518.40 nm with various concentrations of 10 ppm, 20 ppm, 30 ppm, 40 ppm and 50 ppm. The results showed that Buas Buas leaves (Premna serratifolia L.) had an antioxidant activity with IC50 values ??of 20.66 ug / mL.

scholarly journals Effect of Perilla frutescens aqueous extract on free radical production by human neutrophil leukocytes

Medicina ◽  
2008 ◽  
Vol 44 (9) ◽  
pp. 699 ◽  
Author(s):  
Gediminas Žekonis ◽  
Jonas Žekonis ◽  
Renata Šadzevičienė ◽  
Genovaitė Šimonienė ◽  
Egidijus Kėvelaitis
Keyword(s):  
Reactive Oxygen Species ◽  
Free Radical ◽  
Aqueous Extract ◽  
Antioxidant Properties ◽  
Reactive Oxygen ◽  
Perilla Frutescens ◽  
Oxygen Species ◽  
Free Radical Production ◽  
Radical Production ◽  
Neutrophil Leukocytes

Objective. The present study was intended to evaluate the antioxidant properties of aqueous extract of the Perilla frutescens (L.) Britton. Material and methods. The antioxidant properties of Perilla frutescens were analyzed employing neutrophil leukocytes stimulated by the nonopsonized Escherichia coli. The neutrophil leukocytes were affected by adding an aqueous extract of Perilla. The generation of the reactive oxygen species by neutrophil leukocytes was investigated using assessment of luminol- and lucigenin-dependent chemiluminescence. Results. We found out that the treatment of neutrophil leukocytes with the Perilla aqueous extract inhibited the release of reactive oxygen species, measured as luminol- and lucigenindependent chemiluminescence, by about 30% and more than 90%, respectively. Conclusion. The results of this study show that the aqueous extract of the Perilla frutescens inhibits significantly free radical production by neutrophil leukocytes, which was especially obvious when the lucigenin-dependent chemiluminescence assessment method was applied.

scholarly journals Quercitin as an antiviral weapon-A review

2021 ◽  
Vol 9 (1) ◽  
pp. 1-7
Author(s):  
Ramen Kalita ◽  
Kunal Bhattacharya ◽  
Amir Ali ◽  
Satyasish Sandilya
Keyword(s):  
Viral Infections ◽  
Cultured Cells ◽  
Antioxidant Properties ◽  
The Body ◽  
Herpes Simplex Virus 1 ◽  
Free Radical Damage ◽  
Dna And Rna ◽  
Unstable Molecules

Antioxidants are substances that can prevent cells from the damage caused by unstable molecules such as free radicals. Quercetin, a plant pigment present in many fruits, vegetables, grains, and one of the most beneficial antioxidants in the diet and plays an important role in helping the body and prevent free radical damage, which is linked to chronic diseases. The antioxidant properties of quercetin may help to reduce inflammation, allergy symptoms, blood pressure. A lot of studies have been done and experiments have been conducted both in vivo and in vitro and it has been found that in cultured cells many respiratory viruses were inhibited by quercetin. At a minimal inhibitory concentration of 0.03 to 0.5μg/ml in WI-38 or Hela cells, Cytopathic effects produced by echovirus type 7,11,12,19, rhinovirus, poliovirus, and coxsackievirus A21 and B1 were inhibited. The plaque formed by DNA and RNA viruses such as Herpes Simplex Virus-1, Polio type 1, and parainfluenza types 3 were effectively reduced demonstrating its anti-replicative properties. This article reviews effect of quercetin on different types of viral infections.

scholarly journals Factors Influencing the Level of Oxygen Free Radicals in Female Nursing Students

2019 ◽  
Vol 11 (7) ◽  
pp. 1
Author(s):  
Eun-Kyung Kim ◽  
Mi Ryeong Song
Keyword(s):  
Free Radicals ◽  
Free Radical ◽  
Nursing Students ◽  
Oxygen Free Radicals ◽  
Oxygen Free Radical ◽  
The Body ◽  
Eating Habit ◽  
Factors Associated ◽  
Lower Oxygen ◽  
Regression Tests

This study was conducted to investigate the levels of oxygen free radicals and related health factors in 201 female nursing students. The questionnaire was completed by the participants and their oxygen free radical levels were measured by urine test. In this study, an oxygen free radical analyzer was used to measure oxygen free radical levels. The oxygen free radical analyzer analyzes the amount of oxygen free radicals in the body by measuring urinary malondialdehyde (MDA). To determine factors associated with oxygen free radical levels, multiple regression tests were conducted. Of the participants, 89.6% exhibited normal levels of oxygen free radicals and 10.4% had elevated levels. In this study, the factors that affected oxygen free radical levels were eating habit (&beta; = .20, p =. 003), fatigue (&beta; = .20, p = .004), and detox necessity (&beta; = .18, p = .006). In order to lower oxygen free radical levels of female nursing students, the areas of eating habit, fatigue, and detox must receive increased focus.

Gender differences in biochemical measurement reflecting the state of free-radical oxidation and antioxidant protection among workers in metallurgical production

2019 ◽  
Vol 1 (10) ◽  
pp. 877-881
Author(s):  
I. A. Umnyagina ◽  
L. A. Strakhova ◽  
T. V. Blinova
Keyword(s):  
Oxidative Stress ◽  
Gender Differences ◽  
Free Radical ◽  
Biochemical Parameters ◽  
Free Radical Oxidation ◽  
The Body ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Metallurgical Production ◽  
Production Factors

Introduction. To date, age and sex differences have been established for many biochemical parameters. Gender differences in indicators for systems such as antioxidant, thiol-disulfide, oxidative stress and inflammation systems are absent or under study.The aim of the study was to identify gender differences in biochemical parameters reflecting the functioning of antioxidant systems of the body and free radical oxidation in workers of metallurgical production, in contact with harmful production factors.Materials and methods. The blood of men and women working at the metallurgical enterprise of the Nizhny Novgorod region (n=80) under the influence of a complex of physical and chemical production factors was studied. Total oxidative stress, total antioxidant capacity of serum, glutathione levels were studied by photometric biochemical methods. Levels of C-reactive protein and 8-hydroxy–2-deoxyguanosine were studied by ELISA.Results. The average amount of peroxides in the serum of women exceeded 1.6 times this figure in men. In the group of men, the content of 8-Ondg was higher by 26% (p=0.012), the level of GS-by 12% (p=0.019), the activity of SOD — by 1.5–2 times (p=0.0001), the level of CRP — by 2 times (p=0.008) compared to similar indicators in women.Conclusions. Studies of gender differences in workers under the influence of harmful production factors will allow more effective approach to the etiology, treatment and prognosis of production-related diseases. Indicators of oxidative stress and antioxidant protection can be indicators of the health of workers under the influence of harmful industrial factors and be important in the prevention of diseases associated with oxidative stress.

Flavones and Their Analogues as Bioactive Compounds – An Overview

2019 ◽  
Vol 16 (4) ◽  
pp. 377-391 ◽  
Author(s):  
B.S. Jayashree ◽  
H. Venkatachalam ◽  
Sanchari Basu Mallik
Keyword(s):  
Biological Activity ◽  
Free Radical ◽  
Bioactive Compounds ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Cost Effective ◽  
Free Radical Scavenging ◽  
Polyphenolic Compounds ◽  
Pharmacological Activities ◽  
Poor Solubility

Flavonoids constitute a large group of polyphenolic compounds that are known to have antioxidant properties, through their free radical scavenging abilities. They possess a chromone (γ- benzopyrone) moiety, responsible for eliciting many pharmacological activities. Even though, natural flavonoids are highly potent, owing to their poor solubility, they are less used. Therefore, attempts have been made to improve their stability, solubility, efficacy and kinetics by introducing various substituents on the flavone ring. For nearly the last two decades, flavones were synthesized in our laboratory by simple, convenient and cost-effective methods, with the knowledge of both synthetic and semi-synthetic chemistry. In this direction, it was considered worthwhile to present an overview on the synthesized flavonoids. This review creates a platform for highlighting various modifications done on the flavone system along with their biological activity.

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