scholarly journals Keefektifan Asap Cair dan Elektroterapi untuk Mengeliminasi Infeksi Xanthomonas oryzae pv. oryzae pada Benih Padi

2018 ◽  
Vol 14 (2) ◽  
pp. 54
Author(s):  
Rizky G S Purnama ◽  
Kikin Hamzah Mutaqin ◽  
Efi Toding Tondok
Keyword(s):  
Seed Germination ◽  
Bacterial Population ◽  
Seed Quality ◽  
Seed Vigor ◽  
Xanthomonas Oryzae ◽  
Rice Seed ◽  
Liquid Smoke ◽  
Rice Seeds ◽  
Time Required ◽  
Total Seed

The Effectiveness of Liquid Smoke and Electrotherapy to Eliminate Infection of Xanthomonas oryzae pv. oryzae in Rice SeedBacterial leaf blight caused by Xanthomonas oryzae pv. oryzae is a seed-borne disease that can reduce the productivity of rice. Alternative treatments that have not been widely developed is application of liquid smoke and electrotherapy. The aim of this research was to study the effectiveness of liquid smoke and electrotherapy and their combinations to eliminate X. oryzae pv. oryzae without damaging seed quality. The study consisted of three experimental stages: (1) Liquid smoke treatment on the viability of the bacteria and rice seeds; (2) Electrotherapy treatment on the viability of bacteria and rice seeds; (3) Combination of liquid smoke and electrotherapy treatment in bacteria-infected rice seed. The results showed that liquid smoke concentration (5% for 30 minutes) was able to reduce 83.33% population of X. oryzae pv.oryzae on rice seeds with 94.33% seed vigor, 98.00% germination, and time required to achieve 50% of total seed emergence (T50) of 3.13 days. Electrotherapy treatment (400 mA for 20 minutes) was not only eliminated X. oryzae pv. oryzae on rice seeds up to 100% but also maintained seed vigor 81.67%, germination 89.33%, and T50 3.47 days. Electrotherapy treatment (400 mA for 20 minutes) followed by immersion of infected rice seeds in liquid smoke concentration (1% for 30 minutes) could reduce 94.59% bacteria population while maintaining 86.00% seed germination. Treatment of electrotherapy (400 mA for 20 minutes) followed by immersion seeds into liquid smoke concentration (5% for 30 minutes) could reduce bacterial population up to 100%, but caused phytotoxicity effect to the seedling.

scholarly journals Combined Effects of Biological and Chemical Treatment on Rice Seed Physiological and Sanitary Quality

2019 ◽  
Vol 11 (15) ◽  
pp. 106
Author(s):  
Alana Emanoele Pereira ◽  
Weliton Lucas da Silva Benites ◽  
Luana de Carvalho Catelan ◽  
Ana Paula Silva ◽  
Nadia Graciele Krohn
Keyword(s):  
Seed Quality ◽  
Dry Weight ◽  
Seed Vigor ◽  
Rice Seed ◽  
Water Control ◽  
Randomized Design ◽  
Seed Health ◽  
Physiological Quality ◽  
Rice Seeds

This study aimed to evaluate the efficiency of integrated biological and chemical control of pathogens in rice seeds and their effects on seed quality. The experiment was conducted in a 2 × 5 factorial completely randomized design. Fungicide-treated (carboxin/thiram) and untreated rice seeds were inoculated with distilled water (control), Azospirillum brasilense, Bacillus subtilis, Pseudomonas fluorescens, or Trichoderma harzianum. Seed vigor and viability, shoot and root length, and seedling dry weight were determined. The blotter test was carried out to assess seed health. Fungicide treatment improved seed vigor and viability and reduced the incidence of fungi. Biological treatment did not enhance the physiological quality of seeds but was able to control fungi. A. brasilense, B. subtilis, P. fluorescens, and T. harzianum controlled Phoma sorghina; B. subtilis, P. fluorescens, and T. harzianum were effective against Aspergillus flavus; P. fluorescens and T. harzianum controlled Pyricularia oryzae; and T. harzianum was effective against Gerlachia oryzae.

scholarly journals Effect of Seed-Borne Sarocladium Oryzae, the Incitant of Rice Sheath Rot on Rice Seed Quality

2010 ◽  
Vol 50 (1) ◽  
pp. 98-102 ◽  
Author(s):  
Chellappan Gopalakrishnan ◽  
Ayyanar Kamalakannan ◽  
Veeramuthu Valluvaparidasan
Keyword(s):  
Seed Germination ◽  
Seed Quality ◽  
Sugar Content ◽  
Reducing Sugar ◽  
Total Sugar ◽  
Rice Seed ◽  
Seed Infection ◽  
Phenol Content ◽  
Rice Seeds ◽  
Sheath Rot

Effect of Seed-BorneSarocladium Oryzae, the Incitant of Rice Sheath Rot on Rice Seed QualityRice seeds collected fromSarocladium oryzaeinoculated plants produced more discoloured grain, chaffiness and recorded much lower seed germination than seeds collected from healthy plants in all the three cultivars tested. The germination, chaffiness and discolouration in healthy plants were found to be in the range of 70.50 to 93.50 per cent, 5.5 to 17.75 per cent and 4 to 18 per cent respectively. There was a progressive and significant reduction in total sugar, reducing sugar and non reducing sugar content of rice seeds with varying degree of seed infection caused byS. oryzaeThe increase in phenol content due toS. oryzaeinfection ranged from 15.74 to 70.78 per cent and increase being proportional to the per centS. oryzaeseed infection.

scholarly journals Identification of Metabolomic Biomarkers of Seed Vigor and Aging in Hybrid Rice

2021 ◽  
Author(s):  
Bingxian Chen ◽  
Jiadong Gao ◽  
Shijuan Yan ◽  
Yinxin Zhang ◽  
Qi Zhang ◽  
...  
Keyword(s):  
Gluconic Acid ◽  
Seed Quality ◽  
Hybrid Rice ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Primary Metabolism ◽  
Rice Seed ◽  
Seed Aging ◽  
Rice Seeds ◽  
Metabolite Markers

Abstract BackgroundSeed deterioration during rice seed storage will lead to seed vigor loss, which adversely affects agricultural production, the long-term preservation of germplasm resources, and the conservation of species diversity. However, the mechanisms underlying seed vigor maintenance remain largely unknown. ResultsIn this study, 16 hybrid rice combinations were selected from four sterile lines and four restorer lines. Following artificial aging and natural aging treatments, the metabolite markers that could accurately reflect the aging degree of the hybrid rice seeds were identified based on the germination percentage and metabolomics analysis by gas chromatography-mass spectrometry. Significantly differences in the degree of seed deterioration were observed among the 16 hybrid rice combinations tested, with each restorer and sterile lines after storage having the different germination percentage. The hybrid rice combination with the storage-resistant restorer line Guanghui122 exhibited the highest germination percentage under both natural and artificial storage. A total of 89 metabolic peaks and 56 metabolites were identified, most of which were related to primary metabolism. Interestingly, the content of galactose, gluconic acid, fructose and glycerol in the seeds increased significantly during the aging process. Absolute quantification indicated that galactose and gluconic acid were very significantly negatively correlated with the germination percentage of the seeds under the different aging treatments. The galactose content was significantly positively correlated with gluconic acid content. Additionally, while the relative content of raffinose did not change much during storage, a significant positive correlation between raffinose and the germination rate of the artificially aged seeds before storage was detected.ConclusionBased on the metabolomics, metabolite markers which could accurately reflect the aging degree of hybrid rice seeds were identified. Galactose and gluconic acid were very significantly negatively correlated with the germination percentage of the seeds which suggested that these metabolites could constitute potential metabolic markers of seed aging. These findings are of great significance for the rapid and accurate evaluation of seed aging, the determination of seed quality, and the development of molecular breeding approaches for high-vigor rice seeds.

scholarly journals Development of Liquid Enhancer for Germination of Drought-stressed Oryza sativa subsp. indica Seed cv. MR284

2019 ◽  
pp. 1-9 ◽  
Author(s):  
S. N. Mahadi ◽  
F. Zawawi ◽  
R. Nulit ◽  
M. H. Ibrahim ◽  
N. I. Ab. Ghani
Keyword(s):  
Oryza Sativa ◽  
Seed Germination ◽  
Germination Percentage ◽  
Leaf Length ◽  
Seed Vigor ◽  
Rice Seed ◽  
Combination Treatments ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Selection Of

Aim: This study was conducted to develop liquid enhancer containing KCl, TU, GA, and SA for germination of drought-stressed Oryza sativa subsp. indica cv. MR284 seed. Study Design: All experiments were conducted in a completely randomized design. Two steps were involved in the development process which are to select an ideal concentration for each KCl, TU, GA, and SA, and to find an ideal combination of chemicals from the selection of ideal concentrations acquired in step 1 to form liquid enhancer. There were 20 treatments for step 1 and 9 treatments for step 2. All of these treatments with 6 replicates. Place and Duration of Study: Department of Biology, Faculty of Science, University Putra Malaysia, between June 2018 and December 2018. Methodology: The sterilized rice seed cv. MR284 was stressed in the -1.2 Mpa PEG 6000 solution for three days and germinated in the KCl, TU, GA, and SA solution in a series of concentration for 10 days, in a controlled room. Seed germination was observed daily. Results: In the first step, drought-stressed rice seed showed the best germination performance in the 30 mM of KCl, 2.0 mM of TU, 0.24 mM GA, and 0.5 mM SA. Meanwhile, in the second step, the drought-stressed rice seed showed the best germination performance in the combination of 30 mM KCl + 2.0 mM TU + 0.24 mM GA + 0.5 mM SA. The best germination performance was evaluated by the highest germination percentage (%), germination index, seed vigor, leaf length, root length and biomass. Conclusion: Therefore, the combination treatments of 30 mM KCl + 2.0 mM TU + 0.5 mM SA was found to be the most effective and simplest liquid enhancer formula that has an ability to enhance seed germination of drought-stressed rice cv. MR284 seed.

scholarly journals Treatment of rice seeds with salicylic acid: seed physiological quality and yield

2014 ◽  
Vol 36 (3) ◽  
pp. 352-356 ◽  
Author(s):  
Lizandro Ciciliano Tavares ◽  
Cassyo Araújo Rufino ◽  
Sandro de Oliveira ◽  
André Pich Brunes ◽  
Francisco Amaral Villela
Keyword(s):  
Salicylic Acid ◽  
Seed Yield ◽  
Seed Treatment ◽  
Seed Quality ◽  
Rice Seed ◽  
Promising Alternative ◽  
Seed Industry ◽  
Physiological Quality ◽  
Rice Seeds

Seed treatment with growth regulators, especially salicylic acid, is a promising alternative to the seed industry because it is an important inducer of resistance to diseases and pests, as well as acting significantly on quality and seed yield. The objective of this study was to evaluate the performance of rice seed treated with different concentrations of salicylic acid, as well as assess the crop yield and seed quality. The treatments consisted of increasing levels of 0, 50, 100, 150 and 200 mg.L-1 salicylic acid. To this was prepared a stock solution of salicylic acid and the highest concentration by successive dilution in distilled water, the other concentrations were obtained. The physiological quality of seeds produced was treated and evaluated by tests of vigor and germination, and after harvest were evaluated seed yield. It follows that treatment of rice seeds with salicylic acid concentrations up to 130 mg.L-1 at a dose of 2 mL.kg-1 seed does not affect the germination and affects the strength, however provides substantial increases in the yield of seeds. The seed treatment with salicylic acid has no influence on seed quality produced.

Effect of High Temperature Intermittent Drying on Rice Seed Viability and Vigor

2017 ◽  
Vol 13 (10) ◽  
Author(s):  
Pan Wang ◽  
Dong Li ◽  
Li-jun Wang ◽  
Benu Adhikari
Keyword(s):  
High Temperature ◽  
Ambient Temperature ◽  
Seed Viability ◽  
Seed Vigor ◽  
Drying Rate ◽  
Rice Seed ◽  
Rice Cultivars ◽  
Moisture Contents ◽  
Rice Seeds ◽  
Tempering Process

AbstractThis work aimed at determining whether high temperature intermittent drying followed by tempering at ambient temperature could preserve the seed viability and vigor.JaponicaandIndicarice seeds with 21.2 % and 22.6 % wet moisture contents (w.b.) were dried at 50 ºC and 60ºC for either 5, 10, 15 or 20 minutes, followed by tempering at 25ºC for 45 minutes. Each drying cycle was repeated until the rice seeds were dried to 12.0 % (w.b.). The drying rate was improved and the total in-dryer time was reduced in the intermittent drying when compared to continuous drying, due to the tempering process. The seed vigor was significantly reduced by intermittent drying at 60 °C with all exposure times, although the seed still kept the ability to germinate for both rice cultivars. The intermittent drying at 50 ºC for 5 minutes per drying cycle preserved the seed vigor ofJaponicarice well, while inIndicarice, the intermittent drying at 50 ºC up to 20 minutes could still be able to maintain the seed vigor.

scholarly journals Treatment of rice seeds with thiamethoxam: reflections on physiological performance

2014 ◽  
Vol 36 (4) ◽  
pp. 458-464 ◽  
Author(s):  
Andreia da Silva Almeida ◽  
Cristiane Deuner ◽  
Carolina Terra Borges ◽  
Géri Eduardo Meneghello ◽  
Adilson Jauer ◽  
...  
Keyword(s):  
Defense Mechanisms ◽  
Seed Quality ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Cold Test ◽  
Rice Seed ◽  
Seedling Length ◽  
Rice Seeds ◽  
Growing Conditions ◽  
System Length

Thiamethoxam is a systemic insecticide that is transported within the plant through its cells and can activate various physiological reactions such as protein expression. These proteins interact with defense mechanisms against stress in adverse growing conditions. The objective of this study was to evaluate the effect of thiamethoxam in rice seeds and the potential benefits that it can provide. Two experiments were carried out and, in both, seeds were treated with commercial product containing 350 g of thiamethoxam active ingredient per liter of product, at doses 0, 100, 200, 300 and 400 mL.100 kg-1 of seeds: 1) it was conducted with three lots of IRGA BR 424 cultivar rice seeds, which were submitted to the following laboratory tests: germination, cold test, accelerated aging test, as well as field assessment: total seedling length, root system length, number of panicles and productivity; 2) four lots of IRGA BR 424 cultivar rice seeds, two high and two low-vigor, were subjected to the following tests: germination, cold test and greenhouse seedling emergence test. Thiamethoxam rice seed treatment positively favors the seed quality.

scholarly journals ELECTRICAL CONDUCTIVITY AND ETHANOL RELEASE TO ASSESS RED RICE SEED VIGOR1

2021 ◽  
Vol 34 (4) ◽  
pp. 791-798
Author(s):  
RAFAEL MARANI BARBOSA ◽  
MATHEUS ANDRÉ DE JESUS ◽  
RAFAELA ALVES PEREIRA ◽  
GEDEON ALMEIDA GOMES JUNIOR
Keyword(s):  
Electrical Conductivity ◽  
Efficient Method ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Rice Seed ◽  
Seed Sample ◽  
Red Rice ◽  
Germination Test ◽  
Rice Seeds

ABSTRACT To evaluate seed vigor, electrical conductivity and ethanol tests are fast and efficient methodologies. They have the potential to be used in several species, such as red rice. However, there are no protocols or information about their efficiency. Thus, the objective was to evaluate the efficiency, and define parameters of execution for electrical conductivity and ethanol tests, to evaluate the vigor of red rice seeds. The study was conducted using four lots of ‘BRS 901’ red rice, which was subjected to a germination test, as well as first count, accelerated aging, and field seedling emergence tests. The electrical conductivity test was performed with 25, 50, and 100 seeds soaked in 50 mL and 75 mL of water, at 25 °C and 30 °C, for 3, 6, 20, and 24 hours, respectively. The ethanol test was performed with 50 and 100 seeds soaked in a volume of water equivalent to 1.0, 1.5, 2.0, 2.5, and 3.0× the mass of the seed sample. To assess the vigor of red rice seeds, the electrical conductivity test is an efficient method when conducted with 50 seeds soaked in 50 mL of water at 25 °C for 20 hours. Meanwhile, the ethanol test is most effective when performed with 50 seeds, in a volume of water that is 2.5× the mass of the sample, at 40 °C for 24 hours.

scholarly journals Effects of Purple Seed Stain on Seed Quality and Composition in Soybean

Plants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 993
Author(s):  
Richard E. Turner ◽  
M. Wayne Ebelhar ◽  
Teresa Wilkerson ◽  
Nacer Bellaloui ◽  
Bobby R. Golden ◽  
...  
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Seed Germination ◽  
Seed Quality ◽  
Market Price ◽  
Soybean Seed ◽  
Seed Vigor ◽  
Seed Composition ◽  
Infected Seed ◽  
Purple Seed Stain

Purple seed stain disease, caused by (Cercospora kukuchii), is a major concern in soybean (Glycine max (L.)) in Mississippi, USA, due to its effects on seed quality, reducing soybean seed grade and potential market price at elevators. Therefore, investigating the effects of purple seed stain (PSS) on seed quality (germination and vigor) and seed composition (nutrition) is critical. The objective of this study was to investigate the effects of PSS on seed harvest index, seed germination, seed vigor, and seed composition components (protein, oil, fatty acids, and sugars). A field experiment was initiated in 2019 in Stoneville, MS, at the Delta Research and Extension Center (DREC) on a Commerce silt loam soil (fine-silty, mixed, superactive, nonacid, thermic Fluventic Epiaquepts). Soybean variety Credenz 4748 LL was used. The results showed that infected (symptomatic) seed had a 5.5% greater Seed Index (based on 100 seed weight) when compared to non-infected (non-symptomatic, as control) seed. Non-infected seed had greater percent germination and seedling vigor when compared to infected seed. Germination was 30.9% greater and vigor was 58.3% greater in non-infected seed. Also, the results showed that infected seed with PSS had higher protein content and some amino acids. No changes in total oil and fatty acids. Sucrose and stachyose were lower in infected seed than in non-infected seed. The research showed that PSS impacted seed health and seed quality (germination and vigor) and seed composition (protein, sugars, and some amino acids). Purple stained seed should be avoided when planting and should be managed properly as low germination is a potential risk. Planting population should be adjusted accordingly due to lack of germination and vigor if PSS is present. This research help growers for purple seed management, and scientists to further understand the potential negative impact on seed quality and nutrition. Further research is needed before conclusive recommendations are made.

scholarly journals Viability and Dormancy of Rice Seeds after Storage and Pre-Treatment with Dry Heat and Chemical Agents

2017 ◽  
Vol 9 (7) ◽  
pp. 175 ◽  
Author(s):  
Yvonne A. Mutinda ◽  
James W. Muthomi ◽  
John M. Kimani ◽  
George N. Cheminigw’wa ◽  
Florence M. Olubayo
Keyword(s):  
Gibberellic Acid ◽  
Seed Dormancy ◽  
Seed Treatment ◽  
Room Temperature ◽  
Seed Quality ◽  
Packaging Material ◽  
Rice Seed ◽  
Dry Heat ◽  
Rice Seeds ◽  
Pre Treatment

Inappropriate seed packaging and seed dormancy affects seed quality and consequently leads to reduced crop stand, low seedling vigour and reduced yields. The objective of the study was to determine the effect of seed packaging material and seed treatment on viability and dormancy in rice seeds. Effect of packaging material was evaluated by storing freshly harvested seeds of Basmati 370 and BW 196 varieties in polythene, khaki, cheese cloth, polypropylene (Pp) sack and gunny bag sat room temperature and at 6 oC for six months. Effect of seed treatment on dormancy was determined by pre-treatment of seeds with nitric acid at 0.1 M, 0.2 M, 0.5 M, 1 M and 2 M, gibberellic acid (GA3) at 1000 ppm, 2000 ppm and 5000 ppm, and hydrogen peroxide at 0.5 M, 1 M, 2 M, 3 M, 5 M and dry heat at 50 oC for 48 hours. The seeds were subjected to germination test on moist paper and data on percentage germination, seedling length and seedling dry weight were taken. Packaging material had a significant (P ≤ 0.05) effect on germination and vigour. At room temperature, seed stored in polythene bag had the highest germination 88.2% and 57.8% for BW 196 and Basmati 370, respectively. Basmati 370 in khaki bag stored in the cold room had the highest germination 48.3%, while BW 196 stored in polythene had the highest (86.0%). Pre-treatment of seeds reduced dormancy, with variety BW 196 showing the highest germination of up to 67%, while variety Basmati 370 had the lowest germination of up to 40%. Gibberellic acid (GA3) at 5000 ppm was the most effective in breaking seed dormancy resulting in up to 95.7% germination. The results of the study indicated that packaging rice seed in polythene bags for periods not exceeding six months and pre-treatment with GA3 maintains high seed viability and breaks dormancy, respectively, thus ensuring rapid and uniform germination of vigorous seedlings. This would reduce the period farmers have to wait after harvest before they can plant newly harvested rice seeds.

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