The effect of pretreatment using hydrochloric acid on the characteristics of buffalo hide gelatin

The objective of the research was to identify the effect of the concentration of hydrochloric acid (HCl) on yield and the characteristics of gelatin from buffalo hide. A completely randomized design was used with various concentrations of HCl treatment 0.3M, 0.6M, 0.9 M, 1.2 M and 1.5M, and four times replication, followed by least significant difference test. The HCl concentration gave significantly effect (P<0.05) on yield, viscosity, pH, gel strength and redness of gelatin. The highest yield (29.17%) was obtained at a concentration of HCl 0.9M. The highest gel strength and viscosity at concentration of 1.2M, were 293.41g bloom and 22.17 cp, respectively. The pH of the gelatin were 5.08 to 5.52. The lightness level (L*) was 69.92-70.97, whereas the redness ranged (a*) from 0.55 to 1.54 and the yellowness ranged (b*) from 17.54 to 19.59. The moisture, protein and ash concentrations of gelatin were 7.05±0.07-8.92±0.06%, protein content of 83.38±0.06 -91.11±0.03%, and the ash content 0.53 ± 0.04 to 1.23 ± 0.09%, respectively. Based on the spectra absorption of infrared were detected four areas amides: amide A (3402.43-3448.72 cm-1), amide I (1635.65 cm-1), amide II (1527.62 cm-1) and amide III (1242.16 cm-1). The differences in the concentration of HCl had impact on yield,characteristics and secondary structure of buffalo hide gelatin. The HCl concentration at 0.9M was the optimum condition to obtain the highest yield. The characteristics of buffalo hide gelatin after pretreatment using hydrochloric acid had met the standards of GMIA (2012).

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UJI PENGGUNAAN BERBAGAI MEDIA TUMBUH DAN PEMBERIAN PUPUK GROWMORE PADA AKLIMATISASI TANAMAN ANGGREK Dendrobium

Jurnal Ilmiah Pertanian ◽

10.31849/jip.v15i2.1960 ◽

2019 ◽

Vol 15 (2) ◽

pp. 105-114

Author(s):

GUSTI MARLINA ◽

MARLINDA MARLINDA ◽

HENI ROSNETI

Keyword(s):

Plant Height ◽

Growth Media ◽

Growing Media ◽

Randomized Design ◽

Honestly Significant Difference ◽

Significant Difference ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Difference Test ◽

Honestly Significant Difference Test

The research objective was determine the effect of various growth media and growmore fertilizer on the acclimatization of Dendrobium orchids. study used a Completely Randomized Design (CRD) Factorial consisting of 2 factors, the first is (M) of growing media which consists of 3 levels, namely: M1 (medium fern root), M2 (wood charcoal medium), M3 (coir media coconut) and the second is (G) growmore fertilizer which consists of 4 levels, namely: G0 (without Growmore fertilizer), G1 (Growmore fertilizer 2 g / liter), G2 (fertilizer Growmore 4 g / liter), G3 (Growmore fertilizer 6 g / liter). Determine the growth of orchid plants, observations were made with the following parameters Growth Percentage (%), Plant Height (cm), Number of leaves (strands), and Number of tillers (stems). Data obtained were analyzed statistically, then continued with the Honestly Significant Difference Test (BNJ) at the level of 5%. The results showed that the single treatment of the use of growing media did not have a significant effect on the parameters of observation of growth percentage (%), plant height (cm), number of leaves (strands), and number of tillers (stems). The growth of growmore fertilizer also did not show a significant effect on the parameters of observation of growth percentage (%), plant height (cm), number of leaves (strands), and number of tillers (stems). Interaction of the use of various growth media and growmore fertilizer did not show a significant effect on the percentage of growth (%), plant height (cm), number of leaves (strands), and number of tillers (stems).

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Giving Lemna Minor and Mud to the Growth of Tubifex sp.

Budapest International Research in Exact Sciences (BirEx) Journal ◽

10.33258/birex.v1i3.339 ◽

2019 ◽

Vol 1 (3) ◽

pp. 23-27

Author(s):

Mahendra Mahendra ◽

Doni Damara ◽

Mursidatun Nufus ◽

Vira Riana Putri

Keyword(s):

Analysis Of Variance ◽

Lemna Minor ◽

Research Results ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design ◽

Difference Test

This study aims to determine the effect of the use of a combination of lemna minor substrates and mud on the biomass of the population of Tubifex sp. This research is experimental, with a completely randomized design (CRD), with five levels of treatment P1 = 100% (lemna minor ) + 2,000 gr (mud), P2 = 75% (lemna minor ) + 2,000 gr (mud), P3 = 50 % (lemna minor ) + 2,000 gr (mud), P4 = 25% (lemna minor) + 2,000 gr (mud), P5 = 0% (lemna minor) + 2,000 gr (mud). Data obtained from research results in analysis of variance (ANOVA) and in further testing with the Smallest Significant Difference test. The main parameters observed were the number of population and biomass of Tubifex sp. The results of the study showed that the combination of Lemna minor substrate and mud significantly (p <0.05) on the growth of biomass and the population of Tubifex sp. The best results were found in the treatment with the treatment formulation P1 = 100% (lemna minor ) + 2,000 gr (mud) which produced a population of 6,250 ind / gr and biomass 8.60 gr / m2.

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PENGARUH PROPORSI DAMI NANGKA TERHADAP KARAKTERISTIK KIMIA, FISIK DAN ORGANOLEPTIK SELAI LEMBARAN NANAS

Pro Food ◽

10.29303/profood.v6i2.156 ◽

2020 ◽

Vol 6 (2) ◽

pp. 685

Author(s):

Ni Made Neni Parmiutari ◽

Eko Basuki ◽

Rucitra Widyasari

Keyword(s):

Moisture Content ◽

Analysis Of Variance ◽

Chemical Properties ◽

Randomized Design ◽

Yellow Color ◽

Significant Difference ◽

L Value ◽

Completely Randomized Design ◽

Total Dissolved Solid ◽

Difference Test

ABSTRACT This study aims to determine the chemical properties (moisture content, pH and total dissolved solid), physical properties (texture and color) and organoleptics (taste, color, aroma, texture) in the different formulation of slice jam from mixture straw jackfruit and pineapple. This study used a Completely Randomized Design (CRD) with 6 treatments and 3 time replications and analyzed using Analysis of Variance at the 5% level and followed by Least Significant Difference test at the 5% level. These treatments include P0 (100 % pineapple), P1 (80% pineapple: 20% straw jackfruit), P2 (65% pineapple: 35% straw jackfruit), P3 (50% pineapple: 50% straw jackfruit), P4 (35% pineapple: 65% straw jackfruit), dan P5 (20% pineapple: 80% straw jackfruit). Observations made on moisture content, pH, total dissolved solid, texture, color and organoleptic taste, color, texture and aroma (scoring and hedonic). The results showed that the P3 treatment (50% straw jackfruit: 50% pineapple) was the preferred treatment for the panelists on the parameters of color, taste, and texture with the characteristics of moisture content 23.35%, pH 4.86, total dissolved solids 32,46oBrix, L value 44.79 and Hue value 70.67o and brownish yellow color, slightly pineapple taste, chewy texture and pineapple aroma and slightly jackfruit aroma. Keywords: pineapple, slice jam, straw jackfruit ABSTRAK Penelitian ini bertujuan untuk mengetahui sifat kimia (kadar air, pH dan total padatan terlarut), sifat fisik (tekstur dan warna) dan organoleptik (rasa, warna, aroma, tekstur) pada formulasi selai lembaran dari campuran dami nangka dan buah nanas yang berbeda. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dan 3 kali ulangan dan dianalisis menggunakan analisis keragaman (Analysis of Variance) pada taraf 5% dan dilanjutkan dengan uji Beda Nyata Terkecil pada taraf 5%. Perlakuan diantaranya P0 (100 % buah nanas), P1 (80% buah nanas: 20% dami nangka), P2 (65% buah nanas: 35% dami nangka), P3 (50% buah nanas: 50% dami nangka), P4 (35% buah nanas: 65% dami nangka), dan P5 (20% buah nanas: 80% dami nangka). Pengamatan dilakukan terhadap kadar air, pH, total padatan terlarut, tekstur, warna dan organoleptik rasa, warna, tekstur dan aroma (skoring dan hedonik). Hasil penelitian menunjukkan bahwa perlakuan P3 (50% dami nangka : 50% buah nanas) merupakan perlakuan yang agak disukai panelis pada parameter warna, rasa, dan tekstur dengan karakteristik kadar air 23,35%, pH 4,86, total padatan terlarut 32,46oBrix, nilai L 44,79 dan nilai Hue 70,67o serta warna kuning kecokelatan, agak berasa asam nanas, tekstur kenyal dan beraroma nanas dan sedikit beraroma nangka. Kata kunci: buah nanas, dami nangka, selai lembaran

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Pengaruh Waktu Pengomposan Media Tanam terhadap Pertumbuhan dan Hasil Jamur Tiram Putih (Pleorotus ostreatus)

Daun Jurnal Ilmiah Pertanian dan Kehutanan ◽

10.33084/daun.v8i1.2399 ◽

2021 ◽

Vol 8 (1) ◽

pp. 19-28

Author(s):

Heri Kusnayadi ◽

Indra Wira Pratama ◽

Ikhlas Suhada ◽

Nila Wijayanti

Keyword(s):

Vegetable Protein ◽

Fruit Weight ◽

Growth And Yield ◽

Emergence Time ◽

Reduce Body Weight ◽

Randomized Design ◽

Significant Difference ◽

Oyster Mushrooms ◽

Completely Randomized Design ◽

Difference Test

The cultivation of consumption mushrooms in Indonesia is showing an encouraging development. Currently, Indonesia is included as one of the world's major mushroom suppliers. Mushrooms are a source of vegetable protein that does not contain cholesterol and can prevent high blood pressure, heart disease, diabetes, and can reduce body weight. The research objective was to determine the effect of composting time on planting media on the growth and yield of white oyster mushrooms. The research was conducted in the Mapin Kebak Village, sub-district Alas Barat star from May to June 2020. The research was conducted using an experimental method and using a completely randomized design consisting of 5 treatments, where each treatment consisted of three replications. The research treatments consisted of M1 = composting for 6 hours; M2 = composting for 12 hours; M3 = composting for 24 hours; M4 = composting for 36 hours and M5 = composting for 48 hours. Furthermore, the data from the observations were analyzed by ANOVA at the real level of 5%. If the results of the data analysis show a significant difference, then a further test is carried out using the Least Significant Difference Test at the 5% real level. The results of the analysis showed that the fastest time to spread mycelium was in the M5 treatment, the composting time was 48 hours. The fastest primordia emergence time was 24 hours composting M3 treatment. The heaviest fruit weight was in treatment M3 and the lowest fruit weight was in treatment M1.

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14. PENGARUH LAMA PERENDAMAN LARVA DALAM HORMON METIL TESTOSTERON ALAMI TERHADAP PENJANTANAN IKAN LELE DUMBO (Clarias gariepinus) The Effect of Soaking Time of African Catfish Larvae (Clarias gariepinus) in Natural Methyl Testosterone Hormone on Masculinization

Jurnal Medika Veterinaria ◽

10.21157/j.med.vet..v10i2.4629 ◽

2016 ◽

Vol 8 (1) ◽

Author(s):

Rosmaidar R

Keyword(s):

Immersion Time ◽

Clarias Gariepinus ◽

African Catfish ◽

Soaking Time ◽

Experimental Animals ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design ◽

Difference Test ◽

Banda Aceh

The purpose of this study was to determine the effect of immersion time on African catfish (Clarias gariepinus) larvae in natural methyl testosterone hormone on masculinization. This study was conducted in Center of Fish Culture Lamnyong, Banda Aceh. Experimental animals used in this study were 270 African catfish larvae aged 10 days. The study was designed using completely randomized design (CRD) with 3 treatments and 3 replications. African catfish larvae aged 10 days in group P1, P2, and P3 were immersed in natural methyl testosterone hormone for 10 hours, 20 hours, and 30 hours, respectively. Observations were carried out until fish reach 50 days old. Data were analyzed by analysis of variance (Anova) followed by least significant difference test (LSD). Percentage of male catfish observed in P1, P2, and P3 were 64.24 %, 93.11%, dan 59.94% respectively. As a conclusion, immersion with natural methyl testosterone hormone can change the sex of catfish to be male. ____________________________________________________________________________________________________________________ Key words: african catfish, methyl testosterone natural, masculinization

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Isolasi dan Aplikasi Fungi Entomopatogen dari Larva Nyamuk Aedes aegypti L.

JURNAL BIOLOGI PAPUA ◽

10.31957/jbp.833 ◽

2019 ◽

Vol 11 (2) ◽

pp. 87-93

Author(s):

Wuri Artikasari ◽

Emantis Rosa ◽

Bambang Irawan

Keyword(s):

Aedes Aegypti ◽

Entomopathogenic Fungi ◽

Negative Impact ◽

Hemorrhagic Fever ◽

Mosquito Larvae ◽

Chemical Insecticide ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design ◽

Difference Test

DHF (Dengue Hemorrhagic Fever) is a serious problem in Indonesia. DHF disease control has been applied so far, one of which is the use of larvacide temephos (abate). However larvacide is a chemical insecticide that has a negative impact on human health and causes resistance. Therefore in this research, biological control is carried out by utilizing entomopathogenic fungi as a larvacide. The purpose of this study was to determine the effectiveness of entomopathogenic fungi isolated from Ae aegypti larvae. Against the death of Ae. Aegypti mosquito larvae with the moist chamber method. This study used a completely randomized design (CRD) with a factorial pattern and performed two repetitions. Factor A is a type of fungi with 3 levels, namely A1: Aspergillus sp1, A2: Aspergillus sp2, and A3: Syncephalastrum sp. Factor B is a dilution with 7 levels, namely B0: Control, B1: 100 (without dilution), B2: 10-1, B3: 10-2, B4: 10-3, B5: 10-4, B6: 10-5 with every treatment was applied in 2 repetitions. Observations were made 24 hours after treatment for 3 days. Data were analyzed using analysis of variances (anova) and continued with the Least Significant Difference Test (LSD) at 5%. The results indicate that fungi isolates are the most effective in killing Ae. Aegypti mosquito larvae is Aspergillus sp1 and Aspergillus sp2 on the treatment of spores without dilution. Key words: DHF; larvacide; Aedes aegypti; Entomopathogenic Fungi.

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The Effect of Cocopeat and Charcoal Combination in Growing Media of Green Mustard (Brassica rapa L.) Var. Parachinensis Growth

Jurnal Ilmiah Biologi dan Keanekaragaman Hayati ◽

10.23960/jbekh.v7i1.18 ◽

2020 ◽

Vol 7 (1) ◽

pp. 62-66

Author(s):

Masniar Vina Kesti ◽

Bambang Irawan ◽

Priyambodo Priyambodo ◽

Martha Lulus Lande

Keyword(s):

Dry Weight ◽

Growing Media ◽

Randomized Design ◽

Vigor Index ◽

Growing Medium ◽

Significant Difference ◽

Seedling Roots ◽

Completely Randomized Design ◽

Water Gas ◽

Difference Test

Green mustard is one type of vegetable that is easily cultivated and has good prospects to increase farmers' income and community nutrition. Growing media is a material that is useful as a place to stand upright seedlings, to develop seedling roots, to store water, gas, and nutrients needed for seedling growth. The purpose of this study is a). to determine the effect of using a combination of cocopeat and charcoal as a medium for planting mustard greens, b). to get the best dosage of the composition of the growing medium on the growth of mustard greens. The study was conducted in a completely randomized design (CRD) with 4 treatments and 6 replications. The data were analyzed by analysis of variance (ANOVA) and further tested with the Least Significant Difference test (LSD) = 5%. The results showed that the use of cocopeat growing media and charcoal influenced germination and vigor index. Cocopeat and charcoal have a significant effect on fresh and dry weight and chlorophyll content of mustard greens. The mixture of cocopeat growing media and charcoal on P1 did not affect the growth of green mustard on the dry weight. Fresh weight gave a significant effect on K1 and P1 was also significantly different from P2 and P3.

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SIFAT PRO-OKSIDAN SARI JERUK NIPIS (Citrus aurentifolia) TERHADAP AKTIFITAS ANTIOKSIDAN TEH HIJAU (Camellia sinensis)

AGROTECH : JURNAL ILMIAH TEKNOLOGI PERTANIAN ◽

10.37631/agrotech.v1i1.4 ◽

2019 ◽

Vol 1 (1) ◽

pp. 19-26

Author(s):

Sudjatini Sudjatini

Keyword(s):

Optimum Condition ◽

Green Tea ◽

Antioxidative Activity ◽

Radical Scavenging ◽

Optimum Temperature ◽

Randomized Design ◽

Significant Difference ◽

Treatment Factor ◽

Completely Randomized Design ◽

Range Test

The research was designed to understand the effect of addition of extract citrus on the antioxidative activity of green tea and to determine the optimum temperature at the process of green tea extraction to get the best antioxidative activity.Two treatment factors were arranged in completely randomized design with three replications. First treatment factor was the temperature extraction of green tea consisted of 30, 60 and 100 oC, whereas the second treatment factor was concentration of extract citrus consisted of 0.0, 0.2, 0.4, 0.6, 0.8 and 1 %. The contents of polyphenol, tannin, total acids were chemically determined, whereas antioxidative activity was determined by radical scavenging affect (RSA). Data were analyzed using analysis of variance (ANOVA) and Duncan’s Multiple Range Test (DMRT) if there was a significant difference in treatment effects.The research’s results show that there was insignificant interaction between temperature extraction of green tea and concentration of added extract citrus. Eventhough concentration of extract citrus did not significantly affect the contents of polyphenol and tannin but it affected significantly the contents of total acids and antioxidative activity of extract tea. The antioxidative activity at the concentration of extract citrus of 0.2 % was significantly greater than without extract citrus (as check treatment). The following increase in concentration of extract citrus until 1 % did not significantly increase in antioxidative activity. The temperature of tea extraction affected significantly on the contents of polyphenol, tannin, total acids, and antioxidative activity of extract green tea. The highest values were obtained at 100 oC and the the lowest at 30 oC. Temperature extraction of green tea of 100 oC and the addition of 0.2 % extract citrus was the optimum condition. At this condition, the extract green tea contained polyphenol, tannin, total acids, and antioxidative activity as high as 13.77, 12.77, 0.69 and 93.37 %, respectively.

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Growth Response of Soybean Varieties to Trichoderma Application on Acid Soils

E3S Web of Conferences ◽

10.1051/e3sconf/202131603007 ◽

2021 ◽

Vol 316 ◽

pp. 03007

Author(s):

Sutarman ◽

A. Miftahurrohmat

Keyword(s):

Growth Response ◽

Acid Soils ◽

Dry Weight ◽

Randomized Design ◽

Affected Plant ◽

Significant Difference ◽

Wet Weight ◽

Completely Randomized Design ◽

Difference Test ◽

Honest Significant Difference Test

This study aims to determine the growth response of the vegetative stadia of three soybean varieties which are widely used in East Java on acid soils that have been given biological fertilizers from various Trichoderma isolates. The experiment was arranged factorially using a completely randomized design. The first factor was the variety consisting of Detam 3, Detam 4, Gema, Dering 1, and Burangrang. The second factor was Trichoderma isolates formulated as bio fertilizers, consisting of Tc-Jjr-02, Tc-Pjn-01, and Tc-Jro-01. Overall this experiment has 60 experimental units. Data were analyzed by variance analysis (ANOVA) followed by an honest significant difference test at the level of 5%. The results showed that there was no interaction between soybean varieties and Trichoderma isolates formulated in bio fertilizer. Soybean varieties significantly affected plant height, wet weight, and dry weight of roots and stover at 21 days after planting. The Burangrang variety shows the best growing ability in acid soils. There was no interaction between soybean varieties and Trichoderma bio fertilizers. Trichoderma isolate Tc-Jjr-02 increased the wet weight and dry weight of the roots and roots by 48.2 and 54.5%, respectively, and 38.9 and 48.2% compared to without Trichoderma. Trichoderma application maintains soil acidity between pH 4.50-4.67.

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Pengaruh Suplementasi Vitamin C dan Jarak Transportasi Terhadap Penyusutan Bobot Badan Broiler

Jurnal Ilmu Peternakan Terapan ◽

10.25047/jipt.v2i1.1166 ◽

2018 ◽

Vol 2 (1) ◽

pp. 27-33

Author(s):

Nur Kholis ◽

Ujang Suryadi ◽

Faturrahman Roni

Keyword(s):

Heart Rate ◽

Weight Loss ◽

Experimental Design ◽

Vitamin C ◽

Significant Interaction ◽

Randomized Design ◽

Significant Difference ◽

Vitamin C Supplementation ◽

Completely Randomized Design ◽

Difference Test

The purpose of this study was to know the effect of vitamin C supplementation and distance transportation on broiler weight loss. The method used in this study using 128 days of unisexing broiler. The experimental design used a Factorial Completely Randomized Design. The experimental factor consisted of 2 factors, such as the distance factor with 2 levels, 24 km (a1) and 48 km (a2), and the sumplemetation factor of vitamin C with 4 levels such as 0 mg/L (b1), 250 mg/L (b2), 500 mg/L (b3), and 750 mg/L (b4). The experiment was 4 times replication. If obtained significantly result, continued by Least Significant Difference test (LSD). Vitamin C supplementation was significant to weight loss, heart rate, glucose, and mortality in broilers during transportation. The transportation mileage was significant to weight loss and heart rate in broilers. Supplementation of vitamin C and transportation mileage provided a significant interaction weight loss, heart rate, glucose, and mortality in broilers.

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