The features of the composition of the gut microbiota in patients with hypertension and abdominal obesity

The aim of this work was to study the features of the composition of the gut microbiota (GM) in patients with arterial hypertension (AH), which occurs against the background of abdominal obesity (AO). Materials and methods. The paper presents the data on examination of 70 patients with AH of 2–3 degrees (46 patients with AO and 24 patients without obesity – with normal body weight (NBW)). The control group included 20 apparently healthy individuals with NBW. The patients underwent detailed standard clinical, laboratory and instrumental examination. Determination of the quantitative composition of GM was carried out by the method of polymerase chain reaction with hybridization-fluorescence detection of results in real time using the test system “COLONOFLOR-16 (biocenosis)” (“ALFA-LAB”). In this work, a statistical analysis of the obtained data was carried out using standard methods and Microsoft Excel 17.0. Results. A significant change in the composition of GM were found in patients with AH, which proceeds against the background of AO, in comparison with apparently healthy individuals and with patients with AH without obesity (with NBW). In the group of AH patients with the presence of AO, significant decrease in the number of Lactobacillus spp., Bifidobacterium spp., Faecalibacterium prausnitzii and Аkkermansia muciniphila was found in comparison with apparently healthy individuals. The number of Faecalibacterium prausnitzii and Аkkermansia muciniphila in these patients was also significantly lower than in hypertensive patients with NBW. At the same time, the ratio of Bacteroides fragilis/Faecalibacterium prausnitzii was significantly higher than in the control group and the group of hypertensive patients without obesity – with NBW. Conclusions. The results of the study indicate a possible role for the deficiency of such representatives of GM as Lactobacillus spp., Bifidobacterium spp., Faecalibacterium prausnitzii and Аkkermansia muciniphila and an increase in the ratio of Bacteroides fragilis/Faecalibacterium prausnitzii in the pathogenesis of AH, which occurs against the background of AO.

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Effect of Vitamin A-Fortified Rice on the Gut Microbiota of Thai Lactating Women and Their Exclusively Breastfed Infants

Chiang Mai University Journal of Natural Sciences ◽

10.12982/cmujns.2021.079 ◽

2021 ◽

Vol 20 (4) ◽

Author(s):

Lukman Azis ◽

◽

Siwaporn Pinkaew ◽

Santad Wichienchot ◽

◽

...

Keyword(s):

Randomized Controlled Trial ◽

Vitamin A ◽

Gut Microbiota ◽

Controlled Trial ◽

Control Group ◽

Lactating Women ◽

Breastfed Infants ◽

Randomized Controlled ◽

Lactobacillus Spp ◽

Clostridium Spp

Abstract The optimal vitamin A (VA) status of lactating women is important for mothers and their breastfed infants, especially in protecting against infectious diseases. Vitamin A fortified rice is one of the food-base intervention strategy which has the potential to improve VA status. Vitamin A and gut microbiota are interrelated in their effect on human health and immunity however no specific relationship has been proved in these groups of population. This study aimed to determine the effect of VA fortified rice on the gut microbiota changes of lactating woman-exclusively breastfed infant pairs. A double-blind, randomized controlled trial (RCT) of VA fortified rice was conducted in 70 lactating women-infants pairs for 14 weeks. Gut microbiota was measured using the fluorescent in situ hybridization (FISH) and next generation sequencing (NGS) technique. Based on the FISH technique, the numbers of Clostridium spp. /Enterobacter spp. were significantly lower (P < 0.05) in mothers fed VA-fortified rice at the end of the study. In contrast, the abundance of Bifidobacterium spp. and Lactobacillus spp. of infants whose mothers fed with VA-fortified rice was significantly higher (P < 0.05) than the control group. The NGS technique confirmed that results with the increasing of Lactobacillus, B. longum and B. Choerinum in the infant of intervention group. In conclusion, VA-fortified rice was efficacious in decreasing Clostridium spp. /Enterobacter spp. in lactating women and raising the number of Bifidobacterium spp. and Lactobacillus spp. in their breastfed infants. Keywords: Breastfeeding, Gut microbiota, Lactating woman-infant pairs, Randomized controlled trial, Vitamin A

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Study of the Relationship between Microbiome and Colorectal Cancer Susceptibility Using 16SrRNA Sequencing

BioMed Research International ◽

10.1155/2020/7828392 ◽

2020 ◽

Vol 2020 ◽

pp. 1-17 ◽

Cited By ~ 6

Author(s):

Wanxin Liu ◽

Ren Zhang ◽

Rong Shu ◽

Jinjing Yu ◽

Huan Li ◽

...

Keyword(s):

Colorectal Cancer ◽

Gut Microbiota ◽

Cancer Susceptibility ◽

Precancerous Lesions ◽

Bacterial Community Composition ◽

Intestinal Flora ◽

Control Group ◽

Healthy Controls ◽

Healthy Individuals

A lot of previous studies have recently reported that the gut microbiota influences the development of colorectal cancer (CRC) in Western countries, but the role of the gut microbiota in Chinese population must be investigated fully. The goal of this study was to determine the role of the gut microbiome in the initiation and development of CRC. We collected fecal samples of 206 Chinese individuals: 59 with polyp (group P), 54 with adenoma (group A), 51 with colorectal cancer (group CC), and 42 healthy controls (group HC).16S ribosomal RNA (rRNA) was used to compare the microbiota community structures among healthy controls, patients with polyp, and those with adenoma or colorectal cancer. Our study proved that intestinal flora, as a specific indicator, showed significant differences in its diversity and composition. Sobs, Chao, and Ace indexes of group CC were significantly lower than those of the healthy control group (CC group: Sobs, Chao, and Ace indexes were 217.3 ± 69, 4265.1 ± 80.7, and 268.6 ± 78.1, respectively; HC group: Sobs, Chao, and Ace indexes were 228.8 ± 44.4, 272.9 ± 58.6, and 271.9 ± 57.2, respectively). When compared with the healthy individuals, the species richness and diversity of intestinal flora in patients with colorectal cancer were significantly reduced: PCA and PCoA both revealed that a significant separation in bacterial community composition between the CC group and HC group (with PCA using the first two principal component scores of PC1 14.73% and PC2 10.34% of the explained variance, respectively; PCoA : PC1 = 14%, PC2 = 9%, PC3 = 6%). Wilcox tests was used to analyze differences between the two groups, it reveals that Firmicutes (P=0.000356), Fusobacteria (P=0.000001), Proteobacteria (P=0.000796), Spirochaetes (P=0.013421), Synergistetes (P=0.005642) were phyla with significantly different distributions between cases and controls. The proportion of microorganism composition is varying at different stages of colon cancer development: Bacteroidetes (52.14%) and Firmicutes (35.88%) were enriched in the healthy individuals; on the phylum level, the abundance of Bacteroidetes (52.14%-53.92%-52.46%–47.06%) and Firmicutes (35.88%-29.73%-24.27%–25.36%) is decreasing with the development of health-polyp-adenomas-CRC, and the abundance of Proteobacteria (9.33%-12.31%-16.51%–22.37%) is increasing. PCA and PCOA analysis showed there was no significant (P<0.05) difference in species similarity between precancerous and carcinogenic states. However, the composition of the microflora in patients with precancerous lesions (including patients with adenoma and polyp) was proved to have no significant disparity (P<0.05). Our study provides insights into new angles to dig out potential biomarkers in diagnosis and treatment of colorectal cancer and to provide scientific advice for a healthy lifestyle for the sake of gut microbiota.

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ENDOTOXINEMIA IN PATIENTS WITH OBESITY AND NON-ALCOHOLIC FATTY LIVER DISEASE

PROBLEMS OF ENDOCRINE PATHOLOGY ◽

10.21856/j-pep.2021.3.11 ◽

2021 ◽

Vol 77 (3) ◽

pp. 76-82

Author(s):

Galyna Fadieienko ◽

Oleksiy Gridnyev ◽

Inna Kushnir

Keyword(s):

Bacteroides Fragilis ◽

Control Group ◽

Quantitative Composition ◽

Gram Negative Bacteria ◽

Alcoholic Fatty Liver ◽

E Coli ◽

Bacteroides Fragilis Group ◽

Polymerase Chain ◽

Lactobacillus Spp ◽

Alcoholic Fatty Liver Disease

Objective — to study the features of endotoxinemia and its relationship with changes in the intestinal microbial spectrum in obese patients with non-alcoholic fatty liver disease (NAFLD). Materials and methods. 84 patients with obesity and NAFLD were examined. The control group consisted of 20 apparently healthy individuals. The concentration of endotoxin (ET) in the blood serum was determined using the LAL Chromogenic Endpoint Assay kit manufactured by Hycult Biotech (Netherlands), relative quantitative composition of the microbiota at the level of basic phylotypes — by real-time polymerase chain reaction (CFX96Touch (Bio-Rad, USA)) using universal primers for the 16SpPHK gene and taxon-specific primers), quantitative composition of microbiota — using test of the «Colonoflor-16» system (Alfalab, RF) by real-time polymerase chain reaction.Results. Among patients with comorbidity of obesity and NAFLD, there were 40.48 % of males and 59.52 % of females, whose mean age was 53.72 ± 4.61 years. The ET level in the examined patients with comorbidity of obesity and NAFLC was significantly (p < 0.001) higher (1.01 ET/ml) than in the control group (0.60 ET/ml), was significantly (p < 0,05) is higher in women than in men (1.06 and 0.92 UE/ml, respectively) and increased with increasing age of patients (r = 0.30, p < 0.05). The ET level correlated with the relative content of Firmicutes (r = 0.39, p < 0.05) and their ratio in Bacteroidetes (r = 0.29, p < 0.05) and the level of Bifidobacterium spp. (r = 0 .37, p < 0 .05) a nd h ad a n i nverse r elationship w ith t he r elative c ontent o f B acteroidetes ( r = – 0.42, p < 0.01), including Bacteroides fragilis group (r = – 0.43, p < 0.01), Escherichia coli (r = – 0.41, p < 0.01) and total bacterial mass (r = – 0.39, p < 0.05). In the intestinal microbiota of the examined patients, a decrease in the representatives of the Lactobacillus spp. and Bifidobacterium spp. in 84.5 % and 30.9 % of patients, respectively, as well as Bacteroides thetaiotaomicron (88.0 %), Akkermansia muciniphila (79.8 %) and Faecalibacteriumprausnitzii (33.3 %). Whereas the number of gram-negative bacteria increased primarily due to Enterobacter spp, Citrobacter spp (45.2 %), Escherichia coli (19 %), Bacteroides fragilis group (29.8 %). It should be noted that the decrease in the level of A. muciniphila was often accompanied by an increase in the content of enterobacteria, while the excess content of B. fragilis group was accompanied by an increase above the upper limit of E. coli and the presence of anaerobic imbalance in the microbiota. In turn, increasing the number of Enterobacter spp. / Citrobacter spp. It was often accompanied by an increase in the content of E. coli and representatives of the B. fragilis group, against the background of a reduced number of A. muciniphila. Conclusions. The above indicates the presence of metabolic endotoxinemia in patients with comorbidity of obesity and NAFLD against the background of a decrease in the number of gram-positive anaerobic Lactobacillus spp. i Bifidobacterium spp. and bacteria involved in providing the intestinal barrier function (Bacteroides Thetaiotaomicron, Akkermansia muciniphila, Faecalibacterium prausnitzii), which creates conditions for an increased amount of ET into the blood and is especially important against the background of an increase in the content of gram-negative bacteria (representatives of gammaproteobacteria and bacteria of the Bacteroides fragilis), which are the source of ET.

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ARG16GLY POLYMORPHISM IN THE Β2-ADRENOCEPTOR GENE IN PATIENTS WITH BRONCHIAL ASTHMA

Wiadomości Lekarskie ◽

10.36740/wlek202105128 ◽

2021 ◽

Vol 74 (5) ◽

pp. 1200-1203

Author(s):

Vladyslava V. Kachkovska ◽

Anna V. Kovchun ◽

Iryna O. Moyseyenko ◽

Iryna O. Dudchenko ◽

Lyudmyla N. Prystupa

Keyword(s):

Bronchial Asthma ◽

Fragment Length Polymorphism ◽

Control Group ◽

Polymorphism Analysis ◽

Healthy Individuals ◽

Significant Difference ◽

Polymorphic Variants ◽

Polymerase Chain ◽

Arg16gly Polymorphism ◽

Apparently Healthy

The aim: The objective of the study was to analyze the frequency of Arg16Gly polymorphism in the β2 -adrenoceptor (β2 -АR) gene in patients with bronchial asthma (BA) and to assess the association of the polymorphism with BA risk. Materials and methods: We examined 553 BA patients and 95 apparently healthy individuals. Arg16Gly polymorphism in the β2 -АR gene (rs1042713) was determined using polymerase chain reaction-restriction fragment length polymorphism analysis. Statistical analysis of obtained results was performed using SPSS–17 program. Results: It was established that distribution of Arg/Arg, Arg/Gly, and Gly/Gly genotypes for Arg16Gly polymorphism in the β2 -АR gene was 44.2%, 40.0%, 15.8% in the control group vs. 31.3%; 45.7% and 23.0 among BA patients, respectively (χ2 = 6.59; р = 0.037). No significant difference was observed with regards to the distribution of genotypes for Arg16Gly polymorphism in the β2 -АR gene in men and women controls (χ2 = 4.05; р = 0.13) and BA patients (χ2 = 4.34; р = 0.11). BA risk was 1.74 times higher in the minor allele carriers (Arg/Gly + Gly/Gly genotypes) for Arg16Gly polymorphism in the β2 -АR gene. Conclusions: Analysis of Arg16Gly polymorphic variants in the β2-AR gene showed a statistically significant difference in the distribution of Arg/Arg, Arg/Gly, and Gly/Gly genotypes in patients with BA and apparently healthy individuals due to the higher frequency of Arg/Arg genotype in controls and higher frequency of Gly/Gly genotype in patients with asthma. No difference with regard to gender was found in the distribution of genotypes.

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Estimación de la tasa de filtración glomerular en personas con y sin síndrome metabólico

Revista Facultad de Ciencias de la Salud UDES ◽

10.20320/rfcsudes.v3i1.102 ◽

2016 ◽

Vol 3 (1) ◽

pp. 22

Author(s):

María E Lizardo ◽

María del Pilar Navarro ◽

Marqjuly Camacho ◽

Melvimar Alejandra Magallanes-Hernández ◽

Rubsy Gabriela Pacheco-Gutiérrez ◽

...

Keyword(s):

Abdominal Obesity ◽

Cross Sectional Study ◽

Strongly Correlated ◽

Healthy Individuals ◽

Sectional Study ◽

Cross Sectional ◽

Glomerular Function ◽

Síndrome Metabólico ◽

System Risk ◽

Apparently Healthy

Introduction: Metabolic syndrome (MS) is an independent risk factor, which affects the development of chronic kidney disease, so the glomerular filtration rate (GFR) as an indicator of glomerular function in patients with and without MS who attended the outpatient clinic “los Grillitos, sector Caña de Azucar”. Materials and Methods: A comparative, correlational, cross-sectional study was conducted in a non-probability sample of convenience consisting of 60 patients with MS diagnosed according to the criteria Panel ATP III, and 60 apparently healthy individuals, whom the GFR was determined by the Cockcroft-Gault as well as clinical and biochemical parameters for the diagnosis of MS. Results: Out of the total patients evaluated, 37 (30.7%) showed alterations that put them in grades G2 and G3 system risk stratification of CKD, of these 18 and 19 corresponded to patients with and without MS respectively. Glomerular Hyperfiltration (> 120 mil / min) it was found in both groups 28 (46.7%) and 24 (40%) cases of patients with and without MS respectively. The glomerular function was strongly correlated with abdominal obesity and high levels of stress arterial. As for the number of criteria and its relationship to the level of kidney damage present, not a firm to increase the latter with respect to the first (p=0.385) trend was observed. Conclusion: The change in the glomerular function is not directly related to the MS but with its components, specifically abdominal obesity and hypertension.

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The relationship between job enrichment and abdominal obesity: A longitudinal field study of apparently healthy individuals.

Journal of Occupational Health Psychology ◽

10.1037/a0033730 ◽

2013 ◽

Vol 18 (4) ◽

pp. 458-468 ◽

Cited By ~ 9

Author(s):

Yitzhak Fried ◽

Gregory A. Laurence ◽

Arie Shirom ◽

Samuel Melamed ◽

Sharon Toker ◽

...

Keyword(s):

Field Study ◽

Abdominal Obesity ◽

Longitudinal Field ◽

Healthy Individuals ◽

Job Enrichment ◽

The Relationship ◽

Apparently Healthy

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Subfractional Spectrum of Serum Lipoproteins and Gut Microbiota Composition in Healthy Individuals

Microorganisms ◽

10.3390/microorganisms9071461 ◽

2021 ◽

Vol 9 (7) ◽

pp. 1461

Author(s):

Daria A. Kashtanova ◽

Natalia S. Klimenko ◽

Olga N. Tkacheva ◽

Irina D. Strazhesko ◽

Victoria A. Metelskaya ◽

...

Keyword(s):

Gut Microbiota ◽

Abdominal Obesity ◽

Hdl Cholesterol ◽

Microbiota Composition ◽

Healthy Individuals ◽

Serum Lipoproteins ◽

Metabolic Markers ◽

Low Hdl ◽

Microbiota Diversity ◽

Gut Microbiota Composition

Aim: To reveal the relationship between gut microbiota composition and subfractional spectrum of serum lipoproteins and metabolic markers in healthy individuals from Moscow. Methods: The study included 304 participants (104 were men), who underwent thorough preclinical assessment to exclude any chronic disease as well as cardiovascular pathology. Lipoprotein subfractional distribution was analyzed by Lipoprint LDL System (Quantimetrix, Redodno Beach, CA, USA). Gut microbiota composition was assessed by 16S rRNA sequencing of V3-V4 regions. Results: High gut microbiota diversity was positively associated with HDL-cholesterol (C) level and negatively associated with abdominal obesity, BMI, and dyslipidemia. According to selbal analysis, excessive representation of Prevotella spp. was positively associated with IDL-C and LDL-2-C. VLDL-C correlated with Ruminococcus_u/Faecalibacterium_prausnitzii balance. An unexpected positive relationship between LDL-C level and Bifidobacteriaceae_u/Christensenellaceae_u to Bifidobacterium_u balance was found, which may reflect the importance of the integrative microbiota assessment. Low microbiota diversity was associated with obesity, abdominal obesity and low HDL-C level. Conclusions: Gut microbiota imbalance may be one of the components involved in metabolic disorders. The balance of microorganisms and the microbiota diversity may play a more significant role in human health than individual bacterial genera.

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Features of adipokine status in hypertensive patients with chronic kidney disease

KIDNEYS ◽

10.22141/2307-1257.10.3.2021.239590 ◽

2021 ◽

Vol 10 (3) ◽

pp. 137-142

Author(s):

Ya.M. Filenko ◽

O.M. Korzh

Keyword(s):

Blood Pressure ◽

Chronic Kidney Disease ◽

Kidney Disease ◽

Dispersion Analysis ◽

Left Ventricular ◽

Control Group ◽

Myocardial Mass ◽

Hypertensive Patients ◽

Left Ventricular Myocardial Mass ◽

Apparently Healthy

The study aimed at optimization of diagnosis and evaluation of chronic kidney disease (CKD) in hypertensive patients by studying the role of adipokines (leptin, omentin, visfatin, resistin) in patients with hypertension combined with chronic kidney disease. Materials and methods. The study enrolled 100 patients with hypertension of II and III degrees of Stage 2, of which 51 patients were diagnosed with chronic kidney disease. The control group consisted of 20 apparently healthy people. Results. Our study showed that leptin, omentin, resistin, and visfatin levels were significantly higher in patients with essential hypertension (EH) combined with CKD, in contrast to patients with EH without CKD and in the control group. The results of the Kraskel-Wallis dispersion analysis demonstrated that in patients with EH combined with CKD, adipokines significantly correlated with systolic blood pressure (BP), diastolic blood pressure, hypertension degree, body mass index, low-density lipoproteins, thyroglobulin, glomerular filtration rate, creatinine, end-diastolic size, relative wall thickness index, left ventricular myocardial mass, left ventricular myocardial mass index, presence of diastolic dysfunction, type of diastolic function. Conclusions. Hypertensive patients with CKD presented with a significant increase in adipokine levels (leptin, omentin, resistin, visfatin) in the blood compared to patients with EH without CKD (p < 0.05) and apparently healthy individuals (p < 0.05). The data obtained indicate that adipokines (leptin, omentin, resistin, visfatin) have a significant pathogenetic role in patients with hypertension combined with chronic kidney disease.

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Clinical and metabolic parameters, Ca2+, parathormone depending on serum 25(OH)D concentration in hypertensive patients in the West-Ukrainian population

Journal of Education, Health and Sport ◽

10.12775/jehs.2021.11.12.010 ◽

2021 ◽

Vol 11 (12) ◽

pp. 150-157

Author(s):

Marianna Semianiv ◽

Larysa Sydorchuk

Keyword(s):

Vitamin D ◽

Clinical Symptoms ◽

Hypovitaminosis D ◽

Metabolic Parameters ◽

Total Serum ◽

Control Group ◽

Healthy Individuals ◽

The West ◽

Hypertensive Patients ◽

Lipid Panel

Introduction. Low plasma levels of 25-OH vitamin D (25(OH)D) have been associated with an increased prevalence of hypertension. The purpose of the research was to establish the association of clinical and metabolic parameters, Ca2+, parathormone and serum 25(OH)D concentration in hypertensive patients in the West-Ukrainian population. Material and method. 100 subjects with essential arterial hypertension (EAH) and target-organ damaging (2nd stage), moderate, high, very high cardiovascular risk were involved in the case-control study. Among them, 70,84% females, 29,16% males, mean age 57,86±7,81 yo. The control group consisted of 60 healthy individuals. All recruited patients were examined for heart rate, systolic and diastolic blood pressure (SBP, DBP); SCORE, BMI, waist and hip circumference (WC, HC); lipid panel, serum glucose and ionized calcium, 25(OH)D, parathormone levels were studied. Patients were divided into groups depending on total serum 25(OH)D concentration. Results. Low 25(OH)D concentration (<30 ng/ml) in patients with EAH was associated with arrhythmia, headache, sleep disturbance, weakness, fatigue, and increased total 10-year risk for fatal CVD SCORE>5,0%. We found higher SBP and DBP by 9,12% (р=0,014) and 5,17% (р=0,047), higher BMI in men and women – by 20-28,11% (р<0,05), as well as higher values of WC and HC by 8,69-11,92% (р<0,05) in hypertensive patients with hypovitaminosis D. In addition, obesity (BMI≥30,0 kg/m2), hyperglycemia, hypertriglyceridemia was more frequent – by 43% (p<0,05), 43,13% (p<0,001) and 59,37% (p=0,023), respectively. The level of ionized Ca2+ was slightly higher in healthy individuals (p=0,047) with low vitamin D concentration. PTH was compensatory increased by 31,85% (p=0,048) in hypertensive patients with hypovitaminosis D. Conclussions. Hypovitaminosis D is associated with more severe metabolic, hemodynamic disorders and clinical symptoms in hypertensive patients in the West-Ukrainian population.

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Estimation of Nitric Oxide, Malondialdehyde, and Adenosine Deaminase in Serum of Hypertensive Patients and Normotensive Individuals in Erbil City

Tikrit Journal of Pure Science ◽

10.25130/j.v24i3.813 ◽

2019 ◽

Vol 24 (3) ◽

pp. 17

Author(s):

Gulzar I. Ibrahim1 ◽

Saman M. Abdulkareem2 ◽

Lutfiya M. Hasan1

Keyword(s):

Oxidative Stress ◽

Nitric Oxide ◽

Serum Level ◽

Adenosine Deaminase ◽

Control Group ◽

Healthy Individuals ◽

Hypertensive Patients ◽

And Gender

This study was aimed to estimate nitric oxide (NO), malondialdehyde (MDA), and adenosine deaminase (ADA) in the serum of hypertensive patients. Fifty patients (25 males and 25 females) age 40-70 diagnosed with hypertension involved in the study. Fifty healthy individuals, who had no hypertension in the last year, were identified as the control group. NO, MDA and ADA have performed accordingly. MDA was higher with aging and gender in hypertensive patients. Serum level of MDA was higher in females compared with male due to oxidative stress more in female than a male with aging. ADA was higher among hypertensive with aging, though no significant differences among gender. Serum level of NO was lower with aging with no significant differences among gender. http://dx.doi.org/10.25130/tjps.24.2019.043

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