Antibacterial activity of a microemulsion loaded with cephalosporin

Biologia ◽  
2016 ◽  
Vol 71 (7) ◽  
Author(s):  
Mayson H. Alkhatib ◽  
Magda M. Aly ◽  
Ohud A. Saleh ◽  
Hana M. Gashlan
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Activities ◽  
Distilled Water ◽  
Gram Negative Bacteria ◽  
Food Technology ◽  
Cremophor El ◽  
Gram Negative ◽  
Transmission Electron ◽  
Pharmaceutical Industries ◽  
Ethyl Decanoate

AbstractMicroemulsions (MEs), isotropic mixture of water, oil, surfactant and most frequently cosurfactant, have gained great interest in the pharmaceutical industries and food technology due to their great potential to act as antimicrobials as well as nanocarriers for antibiotics. In this study, the antibacterial activities of a ME, composed of 23.5% Cremophor EL, 12.5% transcutol, 30% ethyl decanoate and 34% distilled water, against Gram-positive and Gram-negative bacteria and its potential as a nanocarrier for cephalosporine (CEPH/ME) were assessed. The morphological structures of the ME and CEPH/ME, revealed by transmission electron microscopy, were spherical and their droplet diameters were 15.55 ± 3.17 nm and 10.56 ± 2.32 nm, respectively. ME was found to have great antibacterial activity against

scholarly journals Antibacterial and Cytotoxic Activities of the Sesquiterpene Lactones Cnicin and Onopordopicrin

2011 ◽  
Vol 6 (2) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Sandra M. Bach ◽  
Mario A. Fortuna ◽  
Rodgoun Attarian ◽  
Juliana T. de Trimarco ◽  
César A. N. Catalán ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Sesquiterpene Lactones ◽  
Antibacterial Activities ◽  
Chloroform Extract ◽  
Cytotoxic Activities ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
High Cytotoxicity

The antimicrobial and cytotoxic activities of chloroform extracts from the weeds Centaurea tweediei and C. diffusa, and the main sesquiterpene lactones isolated from these species, onopordopicrin and cnicin, respectively, were assayed. Results show that the chloroform extracts from both Centaurea species possess antibacterial activities against a panel of Gram-positive and Gram-negative bacteria. Remarkable antibacterial activity against methicillin-resistant Staphylococcus aureus was also measured. Both the extracts and the purified sesquiterpene lactones show high cytotoxicity against human-derived macrophages. Despite this cytotoxicity, C. diffusa chloroform extract and cnicin are attractive candidates for evaluation as antibiotics in topical preparations against skin-associated pathogens.

Antibacterial Activity and Chemical Composition of the Essential Oil of Grammosciadium platycarpum Boiss. from Iran

2005 ◽  
Vol 60 (1-2) ◽  
pp. 30-34 ◽  
Author(s):  
Ali Sonboli ◽  
Fereshteh Eftekhar ◽  
Morteza Yousefzadi ◽  
Mohammad Reza Kanani
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Antibacterial Activities ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Two Samples

The chemical composition of the essential oils obtained from two samples (GP1 and GP2) of Grammosciadium platycarpum Boiss. was analyzed by GC and GC-MS. The analysis of the oils resulted in the identification of twenty-two constituents. Linalool (79.0% - GP1, 81.8% - GP2) and limonene (10.0%, 5.8%) were found to be the major components, respectively. The in vitro antibacterial activities of these oils and their main compounds against seven Gram-positive and Gram-negative bacteria were investigated. The results exhibited that the total oils and their major components possess strong to moderate activities against all the tested bacteria except for Pseudomonas aeruginosa.

scholarly journals Antibacterial Activities of Xanthium Strumarium L.

2018 ◽  
Vol 29 ◽  
pp. 70-77 ◽  
Author(s):  
Anjana Devkota ◽  
Ritu Kumari Das
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Xanthium Strumarium ◽  
Distilled Water ◽  
Leaf Extracts ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria

Antibacterial activities of Xanthium strumarium L. (Asteraceae) was carried out in laboratory. Distilled water and methanol extracts of the leaves of plant was prepared. The antibacterial activity was studied against six pathogenic bacteria, three gram negative: Klebsiella pneumoniae (ATCC 15380), Proteus mirabilis (ATCC 49132), Escherichia coli (ATCC 25922) and three gram positive: Bacillus subtilis (ATCC 6633), Enterococcus faecalis (ATCC 29212), Staphylococcus aureus (ATCC 25932) at different concentrations (50 mg/ml, 100 mg/ml, 150 mg/ml, 200 mg/ ml, 250 mg/ml) of leaf extracts of X. strumurium. The phytochemical screening depicted the presence of terpenoids, saponins, flavonoids, tannins and alkaloids. The antibacterial activity of extracts was determined by disc diffusion method and zone of inhibition (ZOI) was measured. Gram negative bacteria was found more resistant than gram positive bacteria. The most susceptible bacterium was S. aureus while the most resistant bacterium was E. coli. Methanolic extract was found more effective than distilled water. These findings suggest that extracts obtained from leaves of X. strumurium possess biobactericidal potential, which can suitably be exploited for making antibacterial drugs.J. Nat. Hist. Mus. Vol. 29, 2015, Page: 70-77

scholarly journals 5-Bromo-1-(4-chlorobenzyl)-1H-indole-2-carboxamides as new potent antibacterial agents

2018 ◽  
Vol 24 (6) ◽  
pp. 327-332 ◽  
Author(s):  
Yogesh D. Mane ◽  
Smita S. Patil ◽  
Dhanraj O. Biradar ◽  
Bhimrao C. Khade
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Antibacterial Agents ◽  
Inhibitory Concentration ◽  
Antibacterial Activities ◽  
Gram Negative Bacteria ◽  
Internal Standards ◽  
Gram Negative ◽  
E Coli ◽  
High Antibacterial Activity

Abstract Ten 5-bromoindole-2-carboxamides were synthesized, characterized and evaluated for antibacterial activity against pathogenic Gram-negative bacteria Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Salmonella Typhi using gentamicin and ciprofloxacin as internal standards. Compounds 7a–c, 7g and 7h exhibit high antibacterial activity with a minimum inhibitory concentration (MIC) of 0.35–1.25 μg/mL. Compounds 7a–c exhibit antibacterial activities that are higher than those of the standards against E. coli and P. aeruginosa.

scholarly journals Synergistic Effects of Anionic/Cationic Dendrimers and Levofloxacin on Antibacterial Activities

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 2894 ◽  
Author(s):  
Natalia Wrońska ◽  
Jean Pierre Majoral ◽  
Dietmar Appelhans ◽  
Maria Bryszewska ◽  
Katarzyna Lisowska
Keyword(s):  
Antibacterial Activity ◽  
Biomedical Applications ◽  
Synergistic Effects ◽  
Antibacterial Activities ◽  
Drug Dosage ◽  
General Interest ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Fluoroquinolone Antibiotics ◽  
Proteus Hauseri

Despite the numerous studies on dendrimers for biomedical applications, the antibacterial activity of anionic phosphorus dendrimers has not been explored. In our research, we evaluated the antibacterial activity of modified polycationic and polyanionic dendrimers in combination with levofloxacin (LVFX) against Gram-negative (Escherichia coli ATCC 25922, Proteus hauseri ATCC 15442) and Gram-positive (Staphylococcus aureus ATCC 6538) bacteria. In the case of Gram-negative bacteria, we concluded that a combination of dendrimers and antibiotic gave satisfactory results due to a synergistic effect. The use of fluoroquinolone antibiotics, such as LVFX, not only caused resistance in disease-causing microorganisms but also increased environmental pollution. Therefore, reduction of drug dosage is of general interest.

The Effects of Natural Dopant Acids on Morphologies and Antibacterial Activity of Polyaniline

2013 ◽  
Vol 650 ◽  
pp. 249-252 ◽  
Author(s):  
Jia Li Mu ◽  
Wen Jun Fan ◽  
Shao Yun Shan ◽  
Ting Wei Hu ◽  
Ya Ming Wang ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Salicylic Acid ◽  
Citric Acid ◽  
Rosin Acid ◽  
Antibacterial Activities ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Bacillus Coli

Polyaniline(PANI) doped by natural acid(citric acid, salicylic acid or rosin acid)solutions was synthesized using aniline as monomer and ammonium peroxydisulfate ((NH4)2S2O8) as oxidant. The antibacterial activities of the PANI against Gram positive bacteria(Bacillus megatherium, Bacillus subtilis, Bacillus cereus and Staphylo-coccus aureus) and Gram negative bacteria(Bacillus coli) were investigated. It was shown that PANI doped by citric acid possessed the better antibacterial activity against the selected species of Gram positive bacteria and Gram negative bacteria than PANI doped by rosin acid. PANI doped by salicylic acid exhibited ascendant antibacterial activities against Gram positive bacteria, but it has little antibacterial activity against the Gram negative bacteria.

scholarly journals Antibacterial Activities of Actinomycete Isolates Collected from Soils of Rajshahi, Bangladesh

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Md. Ajijur Rahman ◽  
Mohammad Zahidul Islam ◽  
Md. Anwar Ul Islam
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Inhibitory Activity ◽  
Pure Culture ◽  
Agar Medium ◽  
Antibacterial Activities ◽  
Soil Samples ◽  
Indicator Bacteria ◽  
Gram Negative Bacteria ◽  
Gram Negative

This study was performed to isolate actinomycete colonies having antibacterial activity from soil samples collected from different places around Rajshahi, Bangladesh. Thirty actinomycete colonies were isolated in pure culture from five soil samples using Starch-casein-nitrate-agar medium. The isolates were grouped in five color series based on their aerial mycelia color and screened for their antibacterial activity against a range of test bacteria. Sixteen isolates (53.3%) were found to have moderate to high activity against four gram-positive and four gram-negative bacteria. Since many isolates showed inhibitory activity against indicator bacteria, it is suggestive that Bangladeshi soil could be an interesting source to explore for antibacterial secondary metabolites.

scholarly journals Antibacterial activity of cobalt(II) complexes with some benzimidazole derivatives

2008 ◽  
Vol 73 (12) ◽  
pp. 1153-1160 ◽  
Author(s):  
S.O. Podunavac-Kuzmanovic ◽  
V.M. Leovac ◽  
D.D. Cvetkovic
Keyword(s):  
Antibacterial Activity ◽  
Chemical Structure ◽  
Antibacterial Activities ◽  
Benzimidazole Derivatives ◽  
Wall Structure ◽  
Gram Negative Bacteria ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative

The antibacterial activities of cobalt(II) complexes with two series of benzimidazoles were evaluated in vitro against three Gram-positive bacterial strains (Bacillus cereus, Staphylococcus aureus, and Sarcina lutea) and one Gram-negative isolate (Pseudomonas aeruginosa). The minimum inhibitory concentration was determined for all the complexes. The majority of the investtigated complexes displayed in vitro inhibitory activity against very persistent bacteria. They were found to be more active against Gram-positive than Gram-negative bacteria. It may be concluded that the antibacterial activity of the compounds is related to the cell wall structure of the tested bacteria. Comparing the inhibitory activities of the tested complexes, it was found that the 1-substituted- -2-aminobenzimidazole derivatives were more active than complexes of 1-substituted- 2-amino-5,6-dimethylbenzimidazoles. The effect of chemical structure on the antibacterial activity is discussed.

scholarly journals Antibacterial Activity of Non-Cytotoxic, Amino Acid-Modified Polycationic Dendrimers against Pseudomonas aeruginosa and Other Non-Fermenting Gram-Negative Bacteria

Polymers ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 1818 ◽  
Author(s):  
Anna Maria Schito ◽  
Silvana Alfei
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Antibacterial Agents ◽  
Antibacterial Activities ◽  
Multidrug Resistant ◽  
Eukaryotic Cells ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Resistance Rates ◽  
New Compounds

Due to the rapid increase of antimicrobial resistance with ensuring therapeutic failures, the purpose of this study was to identify novel synthetic molecules as alternatives to conventional available, but presently ineffective antibiotics. Variously structured cationic dendrimers previously reported have provided promising outcomes. However, the problem of their cytotoxicity towards eukaryotic cells has not been completely overcome. We have now investigated the antibacterial activities of three not cytotoxic cationic dendrimers (G5Ds: G5H, G5K, and G5HK) against several multidrug-resistant (MDR) clinical strains. All G5Ds displayed remarkable activity against MDR non-fermenting Gram-negative species such as P. aeruginosa, S. maltophilia, and A. baumannii (MICs = 0.5–33.2 µM). In particular, very low MIC values (0.5–2.1 µM) were observed for G5K, which proved to be more active than the potent colistin (2.1 versus 3.19 µM) against P. aeruginosa. Concerning its mechanism of action, in time-killing and turbidimetric studies, G5K displayed a rapid non-lytic bactericidal activity. Considering the absence of cytotoxicity of these new compounds and their potency, comparable or even higher than that provided by the dendrimers previously reported, G5Ds may be proposed as promising novel antibacterial agents capable of overcoming the alarming resistance rates of several nosocomial non-fermenting Gram-negative pathogens.

In-vitro Antioxidant and Antibacterial Activity of Methanolic Extract of Shorea robusta Gaertn. F. Resin

2016 ◽  
Vol 6 (4) ◽  
pp. 68
Author(s):  
Sushma Vashisht ◽  
Manish Pal Singh ◽  
Viney Chawla
Keyword(s):  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Gram Positive ◽  
Gram Negative ◽  
Shorea Robusta ◽  
Dose Dependent ◽  
Resin Extract

The methanolic extract of the resin of Shorea robusta was subjected to investigate its antioxidant and antibacterial properties its utility in free radical mediated diseases including diabetic, cardiovascular, cancer etc. The methanol extract of the resin was tested for antioxidant activity using scavenging activity of DPPH (1,1-diphenyl-2-picrylhydrazil) radical method, reducing power by FeCl3 and antibacterial activity against gram positive and gram negative bacteria using disc diffusion method. The phytochemical screening considered the presence of triterpenoids, tannins and flavoniods. Overall, the plant extract is a source of natural antioxidants which might be helpful in preventing the progress of various oxidative stress mediated diseases including aging. The half inhibition concentration (IC50) of resin extract of Shorea robusta and ascorbic acid were 35.60 µg/ml and 31.91 µg/ml respectively. The resin extract exhibit a significant dose dependent inhibition of DPPH activity. Antibacterial activity was observed against gram positive and gram negative bacteria in dose dependent manner.Key Words: Shorea robusta, antioxidant, antibacterial, Disc-diffusion, DPPH.

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