Proximate biochemical composition and mineral content of edible species from the genus Cystoseira in Portugal

AbstractMacroalgae are valuable resources for human consumption in many countries. This work reports for the first time a comparative evaluation of the nutritional properties of five edible macroalgae from the genus

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Hemp (Cannabis sativa L.) Flour-Based Wheat Bread as Fortified Bakery Product

Plants ◽

10.3390/plants10081558 ◽

2021 ◽

Vol 10 (8) ◽

pp. 1558

Author(s):

Iulian Eugen Rusu ◽

Romina Alina Marc (Vlaic) ◽

Crina Carmen Mureşan ◽

Andruţa Elena Mureşan ◽

Vlad Mureşan ◽

...

Keyword(s):

Mineral Content ◽

Unsaturated Fatty Acids ◽

Cannabis Sativa ◽

New Products ◽

Wheat Bread ◽

Human Consumption ◽

Nutritional Properties ◽

Bakery Product ◽

Nutritional Characteristics ◽

Yeasts And Molds

Hemp flour from Dacia Secuieni and Zenit varieties was added to bread in different proportions (5%, 10%, 15% and 20%) to improve its nutritional properties. The purpose of this paper was to present the advanced nutritional characteristics of these bread samples. The selected varieties of hemp, accepted for human consumption, met the requirements for the maximum accepted level of THC in seeds. The protein content of new products increased from 8.76 to 11.48%, lipids increased from 0.59 to 5.41%, mineral content from 1.33 to 1.62%, and fiber content from 1.17 to 5.84%. Elasticity and porosity decreased from 95.51 to 80% and 78.65 to 72.24%, respectively. K, Mg, Ca, P, Mn and Fe are the main mineral substances in bread with addition of hemp flour from the Dacia Secuieni and Zenit varieties. The total amount of unsaturated fatty acids in the bread samples with hemp flour ranged from 67.93 g/100 g and 69.82 g/100 g. Eight amino acids were identified, of which three were essential (lysine, phenylalanine, histidine). Lysine, the deficient amino acid in wheat bread, increased from 0.003 to 0.101 g/100 g. Sucrose and fructose decreased with the addition of hemp flour, and glucose has not been identified. The amount of yeasts and molds decreased in the first 3 days of storage. Regarding the textural profile, the best results were obtained for the samples with 5% addition. In conclusion, bread with the addition of hemp flour has been shown to have superior nutritional properties to wheat bread.

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Insight into the Secondary Metabolites of Geum urbanum L. and Geum rivale L. Seeds (Rosaceae)

Plants ◽

10.3390/plants10061219 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1219

Author(s):

Marek Bunse ◽

Peter Lorenz ◽

Florian C. Stintzing ◽

Dietmar R. Kammerer

Keyword(s):

Fatty Acids ◽

Phenolic Compounds ◽

Unsaturated Fatty Acids ◽

Asiatic Acid ◽

Cosmetic Products ◽

Nutritional Properties ◽

First Time ◽

Derivatives Of ◽

Geum Urbanum ◽

Insight Into

The present study aimed at the identification and quantitation of phenolic compounds, fatty acids, and further characteristic substances in the seeds of Geum urbanum L. and Geum rivale L. For this purpose, individual components of extracts recovered with MeOH, CH2Cl2, and by cold-pressing, respectively, were characterized by HPLC-DAD/ESI-MSn and GC/MS and compared with reference compounds. For both Geum species, phenolic compounds, such as flavonoids and gallic acid derivatives, and triterpenes, such as saponins and their aglycones, were detected. Surprisingly, both Geum species revealed the presence of derivatives of the triterpenoid aglycons asiatic acid and madecassic acid, which were characterized for the first time in the genus Geum. Furthermore, the fatty acids of both species were characterized by GC–MS after derivatization. Both species showed a promising fatty-acid profile in terms of nutritional properties because of high proportions of unsaturated fatty acids. Linoleic acid and linolenic acid were most abundant, among other compounds such as palmitic acid and stearic acid. In summary, the present study demonstrates the seeds of G. urbanum and G. rivale to be a valuable source of unsaturated fatty acids and bioactive phenolics, which might be exploited for nutritional and cosmetic products and for phytotherapeutic purposes.

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Isolation, Characterization and Biotechnological Potentials of Thraustochytrids from Icelandic Waters

Marine Drugs ◽

10.3390/md17080449 ◽

2019 ◽

Vol 17 (8) ◽

pp. 449 ◽

Cited By ~ 2

Author(s):

Magnús Örn Stefánsson ◽

Sigurður Baldursson ◽

Kristinn P. Magnússon ◽

Arnheiður Eyþórsdóttir ◽

Hjörleifur Einarsson

Keyword(s):

Biodiesel Production ◽

Human Consumption ◽

Northern Coast ◽

Omega 3 ◽

Marine Oils ◽

Media Formulation ◽

Omega 6 ◽

First Time ◽

18S Rdna Sequence ◽

Icelandic Waters

The following study reports on the first thraustochytrid isolates identified from Iceland. They were collected from three different locations off the northern coast of the country (Location A, Skagaströnd; Location B, Hveravík; and Location C, Eyjafjörður). Using 18S rDNA sequence analysis, isolates from Locations A and B were identified within the Thraustochytrium kinnei species while other isolates within the Sicyoidochytrium minutum species when compared to other known strains. Cells isolated from Locations A ( 2 . 10 ± 0 . 70 g/L) and B ( 1 . 54 ± 0 . 17 g/L) produced more biomass than the ones isolated from Location C ( 0 . 43 ± 0 . 02 g/L). This study offers the first-time examination of the utility of byproducts from fisheries as a nitrogen source in media formulation for thraustochytrids. Experiments showed that isolates produced more biomass (per unit of substrate) when cultured on nitrogen of marine ( 2 . 55 ± 0 . 74 g/L) as compared to of commercial origin ( 1 . 06 ± 0 . 57 g/L). Glycerol ( 2 . 43 ± 0 . 56 g/L) was a better carbon source than glucose ( 1 . 84 ± 0 . 57 g/L) in growth studies. Fatty acid (FA) profiles showed that the isolates from Location C (S. minutum) had low ratios of monounsaturated ( 4 . 21 ± 2 . 96 % ) and omega-6 ( 0 . 68 ± 0 . 59 % ) FAs. However, the isolates also had high ratios of docosahexaenoic acid (DHA; 35 . 65 ± 1 . 73 % ) and total omega-3 FAs ( 40 . 39 ± 2 . 39 % ), indicating that they could serve as a source of marine oils for human consumption and in aquaculture feeds. The T. kinnei isolates from Location A could be used in biodiesel production due to their high ratios of monounsaturated ( 18 . 38 ± 6 . 27 % ) long chain ( 57 . 43 ± 8 . 27 % ) FAs.

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Mineral content of different bottled water available in Bangladesh: Assessment of their compliance with current regulations

Journal of the Asiatic Society of Bangladesh Science ◽

10.3329/jasbs.v38i1.15316 ◽

2013 ◽

Vol 38 (1) ◽

pp. 7-15 ◽

Cited By ~ 3

Author(s):

Mohammad Arifur Rahman ◽

Md Abdus Salam ◽

Abdus Salam ◽

Madhusudan Roy ◽

Nargis Jahan Ara ◽

...

Keyword(s):

Heavy Metals ◽

Mineral Content ◽

Bottled Water ◽

Human Consumption ◽

Distilled Water ◽

Mineral Contents ◽

Lead And Cadmium ◽

Council Directive ◽

Guideline Values ◽

The University

Nine bottled water brands collected from retail shops and food shops beside the University of Dhaka were analyzed for their major and trace element constituents to ascertain their suitability for human consumption. The results obtained were compared with parametric values (PVs) set by European Community Council Directive 98/83/EC and the guideline values (GVs) recommended by WHO. It was found that the majority of the brands tested were low in mineral contents. Moreover, lower values of hardness, TDS and conductivity than the prescribed limits of WHO showed that water was deficient in essential minerals. Minerals like magnesium, potassium, calcium were present in some cases in such a low concentration that water seemed to be as good as distilled water. In case of heavy metals, only lead and cadmium were found to be below the detection limit (0.001 mg/L) which indicates bottled water brands are better for drinking. DOI: http://dx.doi.org/10.3329/jasbs.v38i1.15316 J. Asiat. Soc. Bangladesh, Sci. 38(1): 7-15, June 2012

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Comparative Evaluation of Commercial Concentration Procedures for Human Intestinal Parasite Detection

Laboratory Medicine ◽

10.1093/labmed/lmy072 ◽

2018 ◽

Vol 50 (3) ◽

pp. 243-248 ◽

Cited By ~ 1

Author(s):

Denis Leméteil ◽

Gilles Gargala ◽

Romy Razakandrainibe ◽

Jean Jacques Ballet ◽

Loic Favennec ◽

...

Keyword(s):

Organic Solvent ◽

Comparative Evaluation ◽

Intestinal Parasite ◽

Solvent Free ◽

Parasite Detection ◽

Parasite Infections ◽

Stool Specimens ◽

Concentration Factors ◽

Commercial Kits ◽

First Time

AbstractBackgroundEffective stool concentration is essential in microscopically based diagnosis of human intestinal parasite infections.ObjectiveTo compare the performances of 4 concentration commercial kits and 1 homemade procedure in 96 clinical stool specimens that tested positive for the detection of 9 helminth and 8 protozoa parasites.MethodsThe presence or absence of parasite forms was microscopically determined under conditions of standard practice. Also, we established the accuracies, concentration factors, and extraction yields.ResultsNo difference was observed between procedures for preconcentration specimens that tested positive. However, for preconcentration specimens that tested negative, we discovered that the homemade procedure was the most effective, and 2 of the 4 commercial kits were discovered to be satisfactory for routine applications.ConclusionsFor all parasites, procedures with biphasic solvents exhibited higher performances than organic solvent-free procedures. For the first time, the effectiveness of commercial concentration kits has been evaluated on several common stool parasites, and the results suggest that improvement of commercial procedures is possible.

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Characterization of Bottled Waters by Multielemental Analysis, Stable and Radiogenic Isotopes

Water ◽

10.3390/w12092454 ◽

2020 ◽

Vol 12 (9) ◽

pp. 2454 ◽

Cited By ~ 1

Author(s):

Tea Zuliani ◽

Tjaša Kanduč ◽

Rok Novak ◽

Polona Vreča

Keyword(s):

Carbonic Acid ◽

Water Source ◽

Major Elements ◽

Mineral Waters ◽

Human Consumption ◽

Geochemical Processes ◽

Soil Zone ◽

Low Concentrations ◽

First Time

Multi-elemental (Ca, Mg, Na, K, Al, As, Ba, Cd, Co, Cr, Cu, Fe, Li, Mn, Mo, Ni, P, Pb, Sb, Se, Tl, V, and Zn) and stable isotope (i.e., δ2H, δ18O, and δ13CDIC) analyses were performed on 13 (8 Slovenian and 5 imported) bottled mineral and spring waters from the Slovenian market. In addition, 87Sr/86Sr isotope ratios were determined for the first time. In all analyzed bottled waters, the majority of elements were present although in low concentrations, and according to EU legislation, all were suitable for human consumption. Also, concentrations of major elements (Ca, Mg, Na, and K) were in general agreement with the values reported on the bottle labels, and any differences were the consequence of the natural variability of the water source used for bottling. The exception was one spring water, for which the source location changed, which was confirmed by the δ2H, δ18O, and δ13CDIC data. Two mineral waters had distinctive elemental compositions due to the particular geology of their recharge areas. The δ13CDIC was also investigated to decipher the carbonate contribution in the bottled waters. The results suggest that dissolution of carbonates and non-equilibrium carbonate dissolution by carbonic acid produced from soil zone CO2 are the predominant geochemical processes influencing the δ13CDIC values of bottled water.

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A Simple and Efficient Method for the Partial Synthesis of Pure (3R,3’S)-Astaxanthin from (3R,3’R,6’R)-Lutein and Lutein Esters via (3R,3’S)-Zeaxanthin and Theoretical Study of Their Formation Mechanisms

Molecules ◽

10.3390/molecules24071386 ◽

2019 ◽

Vol 24 (7) ◽

pp. 1386 ◽

Cited By ~ 2

Author(s):

Eloy Rodríguez-deLeón ◽

J. Jiménez-Halla ◽

José Báez ◽

M. Bah

Keyword(s):

Health Risks ◽

Density Functional ◽

Human Consumption ◽

Formation Mechanisms ◽

Chemical Transformations ◽

Partial Synthesis ◽

One Pot ◽

Commercial Source ◽

The One ◽

First Time

Carotenoids are natural compounds that have important roles in promoting and maintaining human health. Synthetic astaxanthin is a highly requested product by the aquaculture industry, but natural astaxanthin is not. Various strategies have been developed to synthesize this carotenoid. Nonetheless, these approaches have not only provided limited global yields, but its main commercial source also carries several health risks for humans. In this contribution, the one-pot base-catalyzed reaction of (3R,3’R,6’R)-lutein (1) esters has resulted in a successful isomerization process to easily obtain up to 95% meso-zeaxanthin (2), which in turn is oxidized to (3R,3’S)-astaxanthin (3) with a global yield of 68%. The same oxidation performed with UV irradiation (365 nm) for 5 min provided the highest global yield (76%). These chemical transformations have also been achieved with a significant reduction of the health risks associated with its potential human consumption. Furthermore, this is the first time only one of the configurational isomers has been obtained semisynthetically. The poorly understood formation mechanisms of these two compounds were also investigated using Density-Functional Theory (DFT) calculations. These theoretical studies revealed that the isomerization involves a base-catalyzed deprotonation at C-6’, followed by C-4’ protonation, while the oxidation occurs via free radical mechanisms.

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Co-infection with filarial nematodes in Sapajus macrocephalus and Cebus albifrons (Primates: Cebidae) from the Peruvian Amazon

Journal of Helminthology ◽

10.1017/s0022149x18000287 ◽

2018 ◽

Vol 93 (3) ◽

pp. 375-378 ◽

Cited By ~ 1

Author(s):

D.F. Conga ◽

P. Mayor ◽

A.P. Furtado ◽

E.G. Giese ◽

J.N. Santos

Keyword(s):

Original Description ◽

Human Consumption ◽

Peruvian Amazon ◽

New Host ◽

Cebus Albifrons ◽

Filarial Nematodes ◽

New Geographical Record ◽

Thoracic And Abdominal ◽

First Time ◽

New Host Records

AbstractDipetalonema caudispina (Molin, 1858) and D. gracile (Rudolphi, 1809) (Filarioidea: Onchocercidae) are two of six known species of filarial nematodes that parasitize Neotropical non-human primates. Adult filariae were collected from the thoracic and abdominal cavities of 38 of 44 specimens of Sapajus macrocephalus (Spix, 1823) and nine of ten specimens of Cebus albifrons (Humboldt, 1812) (Primates: Cebidae), distributed in the Yavarí-Mirín river basin and used locally for human consumption. Co-occurrence of D. caudispina and D. gracile is reported for the first time, with a prevalence of 18.5% (10 of 54 hosts examined). Our finding of D. caudispina and D. gracile in cebids from the Peruvian Amazon constitutes a new geographical record for both filariae, two new host records for D. caudispina, and the first report of D. gracile in S. macrocephalus. In addition, we provide morphometric data for D. caudispina, complementing the original description, as well as scanning electron microscopy details on the structure of the area rugosa and number of caudal papillae in males.

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Brachylaima spp. (Trematoda) parasitizing Cornu aspersum (Gastropoda) in France with potential risk of human consumption

Parasite ◽

10.1051/parasite/2020012 ◽

2020 ◽

Vol 27 ◽

pp. 15

Author(s):

Claudia Gérard ◽

Armelle Ansart ◽

Nolwenn Decanter ◽

Marie-Claire Martin ◽

Maxime Dahirel

Keyword(s):

North Africa ◽

Intestinal Parasite ◽

Health Hazard ◽

Land Snail ◽

Human Consumption ◽

Geographic Spread ◽

Great Body ◽

Cornu Aspersum ◽

Second Intermediate Host ◽

First Time

The edible land snail Cornu aspersum, native to the Mediterranean coastlines of North Africa, is widely distributed on most continents and often invasive in areas where introduction is recent. This species could contribute to the geographic spread of parasites as demonstrated for Brachylaima spp. These cosmopolitan trematodes may represent a threat to human health, like in Australia where Brachylaima cribbi infects humans. In this study, we demonstrate for the first time the occurrence of Brachylaima spp. in two French populations of C. aspersum, Thorigné-Fouillard (Ille-et-Vilaine), and Arçais (Deux-Sèvres), with an overall prevalence of 10.4% (Thorigné-Fouillard) and 73.3% (Arçais), respectively and a metacercarial intensity on average three times higher in Thorigné-Fouillard (37) than in Arçais (11). Cornu aspersum may act as a first and second intermediate host, as demonstrated in Arçais. The morphometrics of metacercariae, particularly the great body length about 2 mm, discriminate our Brachylaima species from those already described in C. aspersum (B. cribbi in Australia, and B. aspersae, B. llobregatensis and B. mascomai in Europe). Molecular analysis, based on 28S and COI, suggests the occurrence of two species in our study, one of which is probably Brachylaima mesostoma, an intestinal parasite of passeriform birds described in Central Europe. We underline the need for further research to identify species of Brachylaima in France and measure the health hazard of consuming field-collected snails.

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Resistance Profiling and Molecular Characterization of Extended-Spectrum/Plasmid-Mediated AmpC β-Lactamase-Producing Escherichia coli Isolated from Healthy Broiler Chickens in South Korea

Microorganisms ◽

10.3390/microorganisms8091434 ◽

2020 ◽

Vol 8 (9) ◽

pp. 1434 ◽

Cited By ~ 1

Author(s):

Hyun-Ju Song ◽

Dong Chan Moon ◽

Abraham Fikru Mechesso ◽

Hee Young Kang ◽

Mi Hyun Kim ◽

...

Keyword(s):

Escherichia Coli ◽

Phylogenetic Analysis ◽

Resistance Gene ◽

Broiler Chickens ◽

Human Consumption ◽

E Coli ◽

Extended Spectrum ◽

First Time ◽

Pfge Profiles

We aimed to identify and characterize extended-spectrum β-lactamase (ESBL)-and/or plasmid-mediated AmpC β-lactamase (pAmpC)-producing Escherichia coli isolated from healthy broiler chickens slaughtered for human consumption in Korea. A total of 332 E. coli isolates were identified from 339 cloacal swabs in 2019. More than 90% of the isolates were resistant to multiple antimicrobials. ESBL/pAmpC-production was noted in 14% (46/332) of the isolates. Six of the CTX-M-β-lactamase-producing isolates were found to co-harbor at least one plasmid-mediated quinolone resistance gene. We observed the co-existence of blaCMY-2 and mcr-1 genes in the same isolate for the first time in Korea. Phylogenetic analysis demonstrated that the majority of blaCMY-2-carrying isolates belonged to subgroup D. Conjugation confirmed the transferability of blaCTX-M and blaCMY-2 genes, as well as non-β-lactam resistance traits from 60.9% (28/46) of the ESBL/pAmpC-producing isolates to a recipient E. coli J53. The ISECP, IS903, and orf477 elements were detected in the upstream or downstream regions. The blaCTX-M and blaCMY-2 genes mainly belonged to the IncI1, IncHI2, and/or IncFII plasmids. Additionally, the majority of ESBL/pAmpC-producing isolates exhibited heterogeneous PFGE profiles. This study showed that healthy chickens act as reservoirs of ESBL/pAmpC-producing E. coli that can potentially be transmitted to humans.

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