scholarly journals Study of Germination and Seedling Growth of Black Cumin (Nigella Sativa L.) Treated by Hydro and Osmopriming under Salt Stress Conditions

2015 ◽  
Vol 48 (2) ◽  
pp. 69-78 ◽  
Author(s):  
A. Ahmadian ◽  
Y. Shiri ◽  
M. Froozandeh
Keyword(s):  
Salt Stress ◽  
Water Potential ◽  
Seedling Growth ◽  
Nigella Sativa ◽  
Germination Percentage ◽  
Stress Conditions ◽  
Black Cumin ◽  
Osmotic Effect ◽  
Shoot Weight ◽  
Salt Toxicity

Abstract The objective of the study was to determine the responsible factors for germination and early seedling growth due to salt toxicity or osmotic effect and to optimize the best priming treatment for these stress conditions. To study the effect of osmopriming and hydropriming on germination and seedling growth of black cumin (Nigella sativa L.) under salt stress conditions this experiment was conducted at Torbat-Heydariyeh University, Torbat, Iran. The treated seeds (control, hydropriming and ZnSO4) of black cumin were evaluated at germination and seedling growth for tolerance to salt (NaCl and Na2SO4) conditions at the same water potentials of 0.0, -0.3, -0.6, -0.9 and -1.2MPa. Electrical conductivity (EC) values of the NaCl solutions were 0.0, 6.5, 12.7, 18.4 and 23.5 dSm-1, respectively. Results showed that hydropriming increased germination and seedling growth under salt stress. Germination delayed in both solutions, having variable germination with different priming treatments. In NaCl treatment, germination percentage, root and shoot weight, shoot and root length were higher but mean germination time and abnormal germination percentage were lower than Na2SO4, at the same water potential. The root / shoot weight and R/S length enhanced with increase of osmotic potential in both NaCl and Na2SO4 solutions. NaCl had less inhibitor effect on seedling growth than the germination. It was concluded that inhibition of germination at the same water potential of NaCl and Na2SO4 resulted from salt toxicity rather than osmotic effect. The findings of this experiment can be useful and applied to achieve best germination and uniform emergence under field conditions for farmers of medicinal plants.

scholarly journals Sensitivity of Seed Germination to Salt Stress in Black Cumin (Nigella sativa L.)

2018 ◽  
Vol 46 (1) ◽  
pp. 202-205 ◽  
Author(s):  
Panayiota PAPASTYLIANOU ◽  
Nikolia-Nonika BAKOGIANNI ◽  
Ilias TRAVLOS ◽  
Ioannis ROUSSIS
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Nigella Sativa ◽  
Germination Percentage ◽  
Oilseed Crop ◽  
Black Cumin ◽  
Germination Time ◽  
Randomized Design ◽  
Mean Germination Time ◽  
Major Factors

Black cumin is an annual oilseed crop, native to the Mediterranean region and widely used for nutritional and medicinal purposes. Reduced seed germination and early seedling growth under saline conditions are considered as major factors limiting the establishment of crops. The objective of this study was to investigate the effect of salinity due to NaCl on germination of black cumin seeds. A set of experiments was conducted under completely randomized design with four replications of 200 seeds at two different suboptimal germination temperatures (15 and 20 °C) in the dark for 16 days. In order to create salt stress conditions during the germination process, five solutions were used (0 as control, 80, 160, 240 and 320 mM NaCl). The germination performance was evaluated by final germination percentage and mean germination time. The effect of salinity on seed germination percentage and mean germination time was significant at p<0.01. Germination of the controls was 96% and 95% at 15 °C and 20 °C, respectively. The presence of NaCl, regardless of temperature, reduced germination. This reduction was more severe at 15 °C (15%, and 19% germination in cases of 80 and 160 mM concentration respectively) than at 20 °C (65% at 80 mM salinity level) and no germination was observed from 240 mM onwards. The mean germination time increased with increased NaCl levels, especially at the lower temperature of 15 °C. The overall results of this experiment showed the inhibitory effects of salt stress on seed germination parameters of black cumin.

Effect of exogenous nitric oxide (NO) supply on germination and seedling growth of mungbean (cv. Nm-54) under salinity stress

2017 ◽  
Author(s):  
Muhammad Salahuddin ◽  
Fahim Nawaz ◽  
Muhammad Shahbaz ◽  
Muhammad Naeem ◽  
Bilal Zulfiqar ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Seedling Growth ◽  
Dry Matter ◽  
Germination Percentage ◽  
Stress Conditions ◽  
Tolerance Index ◽  
Fresh Weight ◽  
Chlorophyll Contents ◽  
Exogenous Nitric Oxide

The present study evaluated the effects of exogenous NO supply, using sodium nitroprusside (SNP) as a source of NO, on germination and seedling growth of mungbean (Vigna radiata) under salt stress conditions. The results showed that the seeds treated with NO solution (0.2 mM SNP) exhibited 80% and 109% higher germination percentage and germination stress tolerance index (GSI) than untreated seeds (control) under salt stress conditions. Similarly, the seedlings fertigated with NO maintained the highest values of 77.8%, 84.3%, 77.2%, 60.5% and 100.3% for plant height stress tolerance index (PHSI), root length stress tolerance index (RLSI), shoot fresh weight stress tolerance index, root fresh weight stress tolerance index (RFSI) and dry matter stress tolerance index (DMSI), respectively. Moreover, the NO fertigated seedlings maintained 57% higher chlorophyll contents than control seedlings. It is concluded that exogenous NO supply is an effective approach to ensure uniform stand establishment in saline regions of the world.

Salinity Tolerance of Black Gram Cultivars During Germination and Early Seedling Growth

2018 ◽  
Vol 51 (3) ◽  
pp. 51-68 ◽  
Author(s):  
M.K. Hasan ◽  
M.S. Islam ◽  
M.R. Islam ◽  
H.N. Ismaan ◽  
A. El Sabagh
Keyword(s):  
Salt Stress ◽  
Salinity Tolerance ◽  
Seedling Growth ◽  
Negative Impact ◽  
Germination Rate ◽  
Dry Weight ◽  
Germination Percentage ◽  
Shoot Length ◽  
Sand Culture ◽  
Black Gram

Abstract A laboratory experiment regarding germination and seedling growth test was conducted with three black gram genotypes tested under three salinity levels (0, 75 and 150 mM), for 10 days, in sand culture within small plastic pot, to investigate the germination and seedling growth characteristics. Different germination traits of all black gram genotypes, like germination percentage (GP), germination rate (GR), coefficient of velocity of germination (CVG) greatly reduced, as well as mean germination time (MGT) increased with increasing salt stress. At high salt stress, BARI Mash-3 provided the highest GP reduction (28.58%), while the lowest was recorded (15.79% to control) in BARI Mash-1. Salinity have the negative impact on shoot and root lengths, fresh and dry weights. The highest (50.32% to control) and lowest reduction (36.39%) of shoot length were recorded in BARI Mash-2 and BARI Mash-1, respectively, under 150 mM NaCl saline conditions. There were significant reduction of root lengths, root fresh and dry weight, shoot length, shoot fresh and dry weight in all genotypes under saline condition. The genotypes were arranged as BARI Mash-1 > BARI Mash-3 > BARI Mash-2, with respect to salinity tolerance.

scholarly journals Role of L-Ornithine in Mitigation of Salt Stress In Allium Cepa L.

2021 ◽  
Vol 50 (4) ◽  
pp. 1165-1171
Author(s):  
Kürşat ÇAvuşoğlu ◽  
Di̇lek ÇAvuşoğlu
Keyword(s):  
Salt Stress ◽  
Mitotic Index ◽  
Seedling Growth ◽  
Chromosome Aberrations ◽  
Germination Percentage ◽  
Fresh Weight ◽  
Group Iii ◽  
Micronucleus Frequency ◽  
Group I ◽  
Allium Cepa L

Effects of L-ornithine (150 mg/l) on the germination, seedling growth, mitotic index, chromosome aberrations and micronucleus frequency of Allium cepa L. bulbs germinated at 0.125 M salinity were studied. The radicle number of the group III bulbs germinated in the medium with ornithine alone as compared to ones of the group I (control) bulbs which germinated in distilled water medium. But, their germination percentage, radicle length and fresh weight were statistically the same as ones of the group I bulbs. Besides, the micronucleus frequency and chromosomal abnormalities in the root-tip meristematic cells of the group III bulbs showed increased germination compared to ones of the group I bulbs. However, their mitotic index statistically showed the same value as the group I bulbs. Salt stress significantly inhibited the germination and seedling growth of A. cepa bulbs. Moreover, it reduced the mitotic index in the root-meristem cells of the bulbs and fairly increased the number of chromosome aberrations and micronucleus frequency. On the other hand, the inhibitive effect of salt on the germination, seedling growth, mitotic index and micronucleus frequency was dramatically alleviated in varying degrees by ornithine application. But, it was ineffective in reducing the detrimental effect of salinity on the chromosome aberrations. The germination percentage, radicle lenght, radicle number, fresh weight, mitotic index, micronucleus frequency and chromosomal aberrations of the group II seedlings grown in 0.125 M salinity were 27%, 13.5 mm, 18.4, 7.1 g, 5.5, 18.3 and 60.8%, respectively while these values became 68%, 16.4 mm, 16.4, 10.5 g, 15.6, 7.6 and 74.8% in the group IV seedlings treated with L-ornithine. Bangladesh J. Bot. 50(4): 1165-1171, 2021 (December)

scholarly journals Effects of NaCl on Germination and Seedling Growth in Macrotyloma uniflorum and Vigna mungo

2017 ◽  
Vol 5 (2) ◽  
pp. 169-176 ◽  
Author(s):  
SUMAN PANTOLA ◽  
KIRAN BARGALI ◽  
S.S. BARGALI ◽  
VIBHUTI VIBHUTI
Keyword(s):  
Salt Stress ◽  
Seedling Growth ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Vigna Mungo ◽  
Growth Stages ◽  
Tolerance Index ◽  
Abiotic Factor ◽  
Weight Percentage ◽  
Macrotyloma Uniflorum

Among abiotic stresses, drought and salinity are two major determinants due to high magnitude of their impact and wide occurrence. Salinity considerably limits the productivity of crops and thus, considered as the most destructive abiotic factor. In the present study, response of Macrotyloma uniflorum and Vigna mungo to salt (NaCl) stress imposed at germination and seedling growth stages was investigated. The aim of the study was identify the physiological and morphological responses of selected leguminous crop. Seeds were obtained from the healthy plants, surface sterilized and placed under six salt stress levels (0, 4, 8, 12, 16, 20 dsm-1). Complete randomized design with three replicates was used for this experiment and the experiments were conducted during the year 2015 in the glasshouse of Department of Botany, DSB, Campus, Kumaun University, Nainital. In comparison to Macrotyloma uniflorum (77%), higher germination percentage was observed in Vigna mungo (99%) at all salinity levels. In both the species, germination percentage and seedling growth decreased with the increase in salinity stress. Though Vigna mungo showed higher values for root (0.17 g) and shoot dry mass (0.27 g) the dry weight percentage reduction was higher in this species as compared to Macrotyloma uniflorum. Decrease in biomass of seedling with increasing salt stress indicated that the stress not only affected germination but also the growth of seedlings, which indicates that the synthetic ability of seed and biomass of the seedlings was also affected. Seed vigor index declined with the increase in salt concentrations. Outcomes from the study could be helpful in understanding the plant’s nature against different levels of salt stress and that could be economically exploited by various able agencies. At each salinity level, M. uniflorum showed higher salt tolerance index as compared to V. mungo so it can be cultivated in fields with salty soil.

scholarly journals Potasssium chloride as a nutrient seed primer to enhance salt‑tolerance in maize

2012 ◽  
Vol 47 (8) ◽  
pp. 1181-1184 ◽  
Author(s):  
Badar‑uz‑Zaman ◽  
Arshad Ali ◽  
Syed Ishtiaq Hyder ◽  
Muhammad Arshadullah ◽  
Saqib Umar Bhatti
Keyword(s):  
Salt Stress ◽  
Salinity Stress ◽  
Dry Matter ◽  
Germination Rate ◽  
Seed Priming ◽  
Germination Percentage ◽  
Stress Conditions ◽  
Maize Crop ◽  
Mean Germination Time ◽  
Muriate Of Potash

The objective of this work was to determine if KCl could be a useful nutrient primer for safe seed germination in maize crop under salt stress conditions. Seed priming was done using 50 mmol L‑1 of muriate of potash, and germination and seedling growth were evaluated after salt stress with NaCl up to 50 mmol L‑1. Another set of seeds was tested under the same salt stress conditions without priming. Under salinity stress, germination percentage, germination rate index, germination coefficient, and seedling vigor indexes were higher in primed seeds. In unprimed seeds, mean germination time increased, while the germination rate index and the fresh and dry matter mass decreased more sharply with salinity stress. The Na/K ratio was higher in unprimed seeds.

Chemical Priming with Salt and Urea Improves Germination and Seedling Growth of Black Cumin (Nigella sativa L.) under Osmotic Stress

2019 ◽  
Vol 38 (3) ◽  
pp. 1170-1178 ◽  
Author(s):  
Mahdi Ghiyasi ◽  
Sina Siavash Moghaddam ◽  
Reza Amirnia ◽  
Christos A. Damalas
Keyword(s):  
Osmotic Stress ◽  
Seedling Growth ◽  
Nigella Sativa ◽  
Black Cumin ◽  
Chemical Priming

scholarly journals Effects of rhizobial nod factors (Lipo chitooligosaccharide) on seedling growth of maize (Zea mays L.) under salt stress

2018 ◽  
Vol 47 (4) ◽  
pp. 831-837
Author(s):  
D. Udhaya Nandhini ◽  
E. Somasundaram
Keyword(s):  
Salt Stress ◽  
Seedling Growth ◽  
Reducing Sugars ◽  
Amylase Activity ◽  
Growth Parameters ◽  
Sugar Content ◽  
Seed Priming ◽  
Germination Percentage ◽  
Nod Factors ◽  
Nod Factor

Effects of nod factors (lipo chitooligosaccharide) on seedling growth of maize under salt stress have been studied. The study was framed with seed priming using lipo chitooligosaccharide (LCO) a nod factor @ 4 ml/kg of seeds and without priming on maize seedling growth which was exposed to different levels of salinity (0, 2, 4, 6, 8 and 10 dS/m). Salinity negatively influenced the seedling growth of maize. However nod factor treated maize seedlings had relatively higher germination percentage, root length, shoot length, reducing sugars and amylase activity. Correlation analysis revealed positive relationships between seedling growth parameters. Thus, seed priming with nod factor (4 ml/kg) improved the resistance to salinity at seedling stage. NaCl concentrations restricted amylase enzyme activity and reducing sugar content in the germinating seeds. Priming of nod factor/LCO mitigated the restricted effects of salinity on amylase activity and reducing sugars.

scholarly journals EFFECTS OF SALICYLIC ACID PRIMING FOR SALT STRESS TOLERANCE IN WHEAT

2020 ◽  
Vol 2020 (1) ◽  
Author(s):  
MF Ghafoor ◽  
Q Ali ◽  
A Malik
Keyword(s):  
Salicylic Acid ◽  
Salt Stress ◽  
Seedling Growth ◽  
Root Length ◽  
Wheat Variety ◽  
Foliar Spray ◽  
Seed Priming ◽  
Shoot Length ◽  
Stress Conditions ◽  
Wheat Seedlings

The present research experiment was conducted in the greenhouse of the Institute of Molecular Biology and Biotechnology, The University of Lahore for determining the possible involvement of salicylic acid (SA) in seed priming and affects on the seedling growth and development under NaCl treatments in wheat variety ANAJ-2017, Shafaq-2006 and Galaxy-2013. The data was collected for various seedling traits and statistically analyzed, which revealed the significance of results for treatments, salt applications, genotypes and the interactions between salt treatments and genotypes. The lower coefficient of variation was recorded for all studied traits which revealed that there was consistency among the results for salicylic acid applications and salt or NaCl treatments. It was concluded from our study that the application of salicylic acid (SA) under salt (NaCl) stress conditions helps wheat seedlings to withstand and compete with stressful conditions. The study revealed that the seed priming with salicylic acid helps to improve root length, shoot length, seedling moisture percentage and fresh seedling weights. The application of NaCl caused to increase the root length, number of roots and shoot length of wheat while salicylic acid (SA) was applied in foliar spray. The use of water priming shows medium effects for the seedling growth of wheat under salt stress environmental conditions. The wheat variety Galaxy-2013 has shown good performance for most of the studied traits of seedlings under salt stress conditions. It was suggested from our study that the variety Galaxy-2013 may be used under salt stress conditions or salt affected soils to improve grain yield of wheat.

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