scholarly journals Can regular moderate exercise lead to changes in miRNA-146a and its adapter proteins in the kidney of streptozotocin-induced diabetic male rats?

2017 ◽  
Vol 51 (3) ◽  
pp. 145-152 ◽  
Author(s):  
H Oghbaei ◽  
N Ahmadi Asl ◽  
F Sheikhzadeh
Keyword(s):  
Mrna Expression ◽  
Interleukin 1 ◽  
Rna Isolation ◽  
Diabetic Rats ◽  
Male Rats ◽  
Adapter Proteins ◽  
Moderate Exercise ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Male Wistar Rats

AbstractObjective.The aim of this study was to assess whether microRNA-146a and its adapter proteins TNF receptor associated factor6 (TRAF6) and interleukin-1 receptor-associated kinase-1 (IRAK1) may be changed in the kidney of streptozotocin-induced diabetic rats, following regular moderate exercise.Methods.Forty adult male Wistar rats were allocated randomly into four groups (n=10), including sedentary control (SC), sedentary diabetic (SD), healthy sixty-day exercise (H60E), and diabetic sixty-day exercise (D60E) groups. Diabetes was induced by an intraperitoneal injection of 60 mg/kg streptozotocin. After 48 h, blood glucose levels >250 mg/dl was included to diabetic rats. After 2 days of diabetes induction, the exercise protocol began. Animals were exposed to 5 days of consecutive treadmill exercise for 60 min/day with the 22 m/min speed for 60 days. The kidneys of the rats were removed and microRNA was extracted from them using the miRCURYTM RNA isolation kit.Results.In diabetic rats, statistical analysis revealed a significant decrease in miR-146a expression, non-significant decrease in IRAK1 mRNA expression, and non-significant increase in TRAF6 and NF-kB mRNA expression compared to the SC group. Exercise led to a non-significant increase in the expression of miR-146a and NF-kB mRNA in the kidneys of the diabetic group as compared to the SD group, significant increase in TRAF6 and IRAK1 mRNA expression compared to the H60E group, and significant increase in TRAF6 mRNA expression compared to the SD group.Conclusion.The present data indicate that exercise might be able to help in the prevention in the diabetic nephropathy development.

scholarly journals Effects of Apocynin on Heart Muscle Oxidative Stress of Rats with Experimental Diabetes: Implications for Mitochondria

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 335
Author(s):  
Estefanía Bravo-Sánchez ◽  
Donovan Peña-Montes ◽  
Sarai Sánchez-Duarte ◽  
Alfredo Saavedra-Molina ◽  
Elizabeth Sánchez-Duarte ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Insulin Sensitivity ◽  
Cardiac Tissue ◽  
Diabetic Rats ◽  
Oxidase Inhibitor ◽  
Beneficial Effects ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Transport Chain ◽  
Male Wistar Rats

Diabetes mellitus (DM) constitutes one of the public health problems today. It is characterized by hyperglycemia through a defect in the β-cells function and/or decreased insulin sensitivity. Apocynin has been tasted acting directly as an NADPH oxidase inhibitor and reactive oxygen species (ROS) scavenger, exhibiting beneficial effects against diabetic complications. Hence, the present study’s goal was to dissect the possible mechanisms by which apocynin could mediate its cardioprotective effect against DM-induced oxidative stress. Male Wistar rats were assigned into 4 groups: Control (C), control + apocynin (C+A), diabetes (D), diabetes + apocynin (D+A). DM was induced with streptozotocin. Apocynin treatment (3 mg/kg/day) was applied for 5 weeks. Treatment significantly decreased blood glucose levels and insulin resistance in diabetic rats. In cardiac tissue, ROS levels were higher, and catalase enzyme activity was reduced in the D group compared to the C group; the apocynin treatment significantly attenuated these responses. In heart mitochondria, Complexes I and II of the electron transport chain (ETC) were significantly enhanced in the D+A group. Total glutathione, the level of reduced glutathione (GSH) and the GSH/ oxidized glutathione (GSSG) ratio were increased in the D+A group. Superoxide dismutase (SOD) and the glutathione peroxidase (GSH-Px) activities were without change. Apocynin enhances glucose uptake and insulin sensitivity, preserving the antioxidant defense and mitochondrial function.

scholarly journals Evaluation of ameliorative effect of sodium nitrate in experimental model of streptozotocin-induced diabetic neuropathy in male rats

2019 ◽  
Vol 53 (1) ◽  
pp. 14-25 ◽  
Author(s):  
Hajar Oghbaei ◽  
Mohammad Reza Alipour ◽  
Gisou Mohaddes ◽  
Gholam Reza Hamidian ◽  
Rana Keyhanmanesh
Keyword(s):  
Serum Insulin ◽  
The Body ◽  
Male Rats ◽  
Diabetes Group ◽  
Insulin Levels ◽  
Nitrate Treatment ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Ameliorative Effect ◽  
Male Wistar Rats

AbstractObjective. Diabetes induces sensory symptoms of neuropathy as positive (hyperalgesia), negative (hypoalgesia), or both.Methods. In the present study, fifty male Wistar rats were allocated to five groups: control, control+nitrate, diabetes, diabetes+insulin, and diabetes+nitrate. Thirty days after diabetes confirmation, insulin (2–4 U/day) was injected subcutaneously in diabetes+insulin group and nitrate (100 mg/l) was added into drinking water of the control+nitrate and diabetes+nitrate groups for a period of 2 months. In order to assess the mechanical and thermal algesia, tail immersion, hot plate, and von Frey tests were performed. The serum insulin levels were determined with insulin ELISA Kit. Serum level of NOx was determined by the Griess method.Results. Both thermal and mechanical nociceptive thresholds showed a significant decrease (p<0.05) which was followed by a significant increase (p<0.01) in the thermal nociceptive threshold in the diabetes group. Chronic nitrate or insulin treatment led to a significant decrease (p<0.01) in blood glucose levels, as well as a significant (p<0.05) increase in the body weight and serum NOx. Moreover, nitrate treatment significantly increased serum insulin levels (p<0.001) compared to the other groups.Conclusion. Chronic nitrate treatment modified the thermal and mechanical sensitivities in diabetic animals.

Hypoglycemic properties of pectine from pumpkin (Cucurbita maxima d.) in a model of alloxan-induced type 1 diabete mellitus

2018 ◽  
pp. 82-89
Author(s):  
Т.В. Федорова ◽  
А.А. Торкова ◽  
К.В. Лисицкая ◽  
И.Б. Алчинова
Keyword(s):  
Antioxidant Properties ◽  
Diabetic Rats ◽  
Hypoglycemic Agents ◽  
Initial Symptom ◽  
Cucurbita Maxima ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Male Wistar Rats ◽  
Alloxan Monohydrate ◽  
Experimental Group

Биохимическим маркером сахарного диабета и его тяжелейших осложнений является гипергликемия. В качестве новых средств растительного происхождения, обладающих гипогликемической активностью, в настоящее время рассматривают пектины. Цель исследования - оценка функциональных свойств пектина, полученного из тыквенного жома с использованием кавитационно-мембранной технологии. Методика. Использована модель аллоксан-индуцированного сахарного диабета. Эксперимент проводили на крысах - самцах Wistar (инъекции раствора аллоксана моногидрата из расчета 43 мг/кг массы). Развитие диабета подтверждалось уровнем глюкозы (>10 мМ) в периферической крови. Для тестирования гипогликемической активности тыквенного пектина опытной группе крыс внутрижелудочно с помощью металлического зонда (Kent Scientific, США) вводили 5% раствор пектина в дистиллированной воде в дозировке 25 мг/100 г живой массы в сутки в течение 3 нед. Пектин, полученный из тыквенного жома с использованием кавитационно-мембранной технологии, имел следующие характеристики: диапазон молекулярных масс 90-120 кДа, содержание полигалактуроновой кислоты в среднем около 75% и степень этерификации 72%, что позволяет его отнести к пектинам с высокой степенью этерификации. Результаты. У животных 2-й и 3-й групп по истечении 1-3 нед. после воспроизведении диабета выявлена гипергликемия - уровень глюкозы в цельной крови был значимо выше. Статистический анализ «size effect» показал, что в начале эксперимента различия в уровне глюкозы в крови животных 2-й и 3-й групп незначительны (d = 0,39). Через 3 нед. они достигают среднего эффекта (d = 0,50). Этот факт можно расценивать как тенденцию к нормализации уровня глюкозы на фоне приема тыквенного пектина. Значимое снижение сывороточной концентрации фруктозамина в опытной группе показало гипогликемический эффект тыквенного пектина. Введение пектина животным также снижало содержание холестерина в печени и сывороточную концентрацию неэстерефицированных жирных кислот (НЭЖК), демонстрируя его гипохолестеринемические свойства. Антиоксидантные свойства пектина проявлялись нормализацией уровня ТБК-реактивных продуктов в сыворотке крови опытной группы животных. Заключение. Подтверждены гипогликемический, гипохолестеринемический и антиоксидантный эффекты пектина из тыквы (Cucurbita maxima D.) при аллоксан-индуцированном сахарном диабете. Hyperglycemia is a biochemically defined initial symptom of diabetes and its serious complications (atherosclerosis, retinopathy, kidney damage). Pectins are currently considered as novel plant-produced hypoglycemic agents. The aim of this study was to evaluate bio-functional properties of pumpkin pectin obtained from pumpkin pulp using cavitation-membrane technologies in a model of alloxan-induced diabetes. Methods. Male Wistar rats were used in the experiments. Diabetes was modeled by injections of 4.3% alloxan monohydrate solution. The development of diabetes was confirmed by glucose concentration in peripheral blood (glucose levels of >10 mM in whole blood was consistent with diabetes). To test the hypoglycemic activity of pumpkin pectin, a 5% pectin solution in distilled water was administered to rats of the experimental group (25 mg/100 g body weight, daily, for three weeks) through a metal gastric tube (Kent Scientific, USA). Results. A significant decrease of fructosamine concentration observed in the experimental group indicated a hypoglycemic effect of pectin. Administration of pectin to animals also reduced concentrations of cholesterol in liver and non-esterified fatty acids (NEFAs) in blood serum, which demonstrated cholesterol-lowering properties of pectin. Antioxidant properties of pectin provided a decrease in serum TBA-reactive products to the level observed in non-diabetic rats, in pectin-treated diabetic animals compared to the untreated diabetic group.

Dioscorea dumetorum-Fed Rats Exhibited Decreased Body Weight, Blood Glucose, and Insulin in STZ-induced Diabetes

2014 ◽  
Vol 4 (2) ◽  
pp. 87 ◽  
Author(s):  
Henrietta Aritetsoma Ogbunugafor ◽  
Emmanuel Emeka Ilodigwe ◽  
Daniel Lotanna Ajaghaku ◽  
Chinwe Nonyelum Ezekwesili ◽  
Chike Samuel Okafor ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Feed Intake ◽  
Weight Reduction ◽  
Blood Glucose Concentration ◽  
Insulin Level ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Male Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels

Background: Preventive measures that could slow down the rising incidences of diabetes mellitus are essential. The use of neglected local foods, which have effects on this chronic disease beyond basic nutrition as dietary controls, is desirable.Objective: The effect of Dioscorea dumetorum (Kunth) Pax (Dioscoreaceae) feed on satiety, weight, blood glucose, and insulin levels were investigated in streptozotocin-induced diabetic rats.Methods: Twenty adult male rats in four groups of five were used for the experiment. Three groups – D. dumetorum, glibenclamide, and standard pellet-fed rats were induced with diabetes by i.p. administration of 50mg kg-1 streptozotocin, while the fouth group (?) served as a non-diabetic control. D. dumetorum was fed at 15g daily for ten days before induction, and after induction, feeding continued. Glibenclamide was orally administered 5mg kg-1 daily. Both the untreated and non-diabetic rats were kept on standard rat pellets. Feed intake, weight, and blood glucose concentration were monitored daily, while insulin level was measured on day two and day six after inductions. Results: Average feed intake for non-diabetic rats was 15g for D. dumetorum per day, which dropped to 10.3g after induction of diabetes. Weight of normal non-diabetic rats consistently increased (142.61 ± 4.37g – 169.43 ± 8.61g) for the duration (17 days) of the experiment. The D. dumetorum-fed rats showed weight reduction of 5.4%, glibenclamide 4.0%, and untreated diabetic 6.15%. Non-diabetic rats blood glucose levels ranged between 70 to 100mg dL-1. Streptozotocin (STZ) (i.p.) administration increased blood glucose levels from 370% to 626% in the rats. D. dumetorum-fed rats showed reduced (p<0.05) blood glucose levels of 22.6%. Glibenclamide had 5.5% reduction (p<0.05). Insulin was absent in D. dumetorum-fed rats, whereas 0.95ng ml-1 of insulin was detected in glibenclamide-administered rats. These quantities were lower (p<0.001) than 1.40ng ml-1 in the non-diabetic rats. Conclusion: This study revealed that D. dumetorum tuber caused decreased hunger, weight reduction, and displayed hypoglycemic property in diabetic rats, even after heat treatment. Its probable mechanism of anti-hyperglycemic activity might not be through increased insulin secretion.Key words: D. dumetorum, streptozotocin-induced diabetes, weight, blood glucose, insulin.

Modulation of GLUT4 expression by oral administration of Mg2+ to control sugar levels in STZ-induced diabetic rats

2014 ◽  
Vol 92 (6) ◽  
pp. 438-444 ◽  
Author(s):  
Haniah Solaimani ◽  
Nepton Soltani ◽  
Kianoosh MaleKzadeh ◽  
Shahla Sohrabipour ◽  
Nina Zhang ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Magnesium Sulfate ◽  
Mrna Expression ◽  
Insulin Level ◽  
Diabetic Rats ◽  
In Vivo Studies ◽  
Glucose Levels ◽  
Blood Glucose Levels

It has been previously shown that oral magnesium administration decreases the levels of glucose in the plasma. However, the mechanisms are not fully understood. The aim of this study was to determine the potential role of GLUT4 on plasma glucose levels by orally administering magnesium sulfate to diabetic rats. Animals were distributed among 4 groups (n = 10 rats per group): one group served as the non-diabetic control, while the other groups had diabetes induced by streptozotocin (intraperitoneal (i.p.) injection). The diabetic rats were either given insulin by i.p. injection (2.5 U·(kg body mass)–1·day–1), or magnesium sulfate in their drinking water (10 g·L–1). After 8 weeks of treatment, we conducted an i.p. glucose tolerance test (IPGTT), measured blood glucose and plasma magnesium levels, and performed in-vitro and in-vivo insulin level measurements by radioimmunoassay. Gastrocnemius (leg) muscles were isolated for the measurement of GLU4 mRNA expression using real-time PCR. Administration of magnesium sulfate improved IPGTT and lowered blood glucose levels almost to the normal range. However, the insulin levels were not changed in either of the in-vitro or in-vivo studies. The expression of GLU4 mRNA increased 23% and 10% in diabetic magnesium-treated and insulin-treated groups, respectively. Our findings suggest that magnesium lowers blood glucose levels via increased GLU4 mRNA expression, independent to insulin secretion.

scholarly journals Profil Antioksidan Darah Tikus Diabetes dengan Asupan Beras Merah yang Diperkaya Kappa-Karagenan dan Ekstrak Antosianin

2017 ◽  
Vol 37 (1) ◽  
pp. 82 ◽  
Author(s):  
Nurhidajah Nurhidajah ◽  
Mary Astuti ◽  
Sardjono Sardjono ◽  
Agnes Murdiati
Keyword(s):  
Blood Glucose ◽  
Diabetic Rats ◽  
Red Rice ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Reducing Ability ◽  
Male Wistar Rats ◽  
Kappa Carrageenan ◽  
Plasma Antioxidant

This study aimed to analyze the effect of red rice enriched-kappa-carrageenan and anthocyanin extracts on blood antioxidant profile in diabetic rats. Variables analyzed in this research were blood glucose, malondialdehyde (MDA) level, and plasma antioxidant by Ferric Reducing Ability of Plasma (FRAP) method. This study was conducted in vivo on male Wistar rats aged 2.5 months using completely randomized design. Rats divided into 6 groups based on types of feed, standard feed (normal and DM), red rice (BM), red rice enriched kappa-carrageenan (BMK), red rice enriched extracts of anthocyanin (BMA) and red rice enriched with kappa-carrageenan and extract anthocyanin (BMKA). Experiments were carried out for 6 weeks. Rats feed with red rice showed decreased in blood glucose levels from 234.26 to 84.78 mg/dL (p = 0.000), MDA diabetic group compared to BMKA 2.175 and 0.530 μmol/L (p = 0.000) respectively, and the rate of FRAP in DM and BMKA 69 and 216 nmol/mL (p = 0.000) respectively. This study showed that red rice enriched with kappa-carrageenan and anthocyanin extract was able to decrease blood glucose levels and increase plasma antioxidant of diabetic rats which characterized by decreased MDA value and increased FRAP value. ABSTRAKPenelitian ini bertujuan mengkaji pengaruh pemberian beras merah yang diperkaya kappa-karagenan dan ekstrak antosianin terhadap profil antioksidan darah pada tikus Diabetes Melitus (DM). Indikator penelitian adalah penurunan glukosa darah dan angka Malondialdehid (MDA) serta peningkatan antioksidan plasma dengan metode Ferric Reducing Ability of Plasma (FRAP). Penelitian ini dilakukan secara in vivo pada hewan coba tikus Wistar usia 2,5 bulan dengan desain penelitian rancangan acak lengkap (RAL). Tikus dibagi 6 kelompok pakan, yaitu standar negatif dan positif (normal dan DM), beras merah (BM), beras merah ditambah kappa-karagenan (BMK), beras merah ditambah ekstrak antosianin (BMA), dan beras merah ditambah kappa-karagenan dan ekstrak antosianin (BMKA). Percobaan dilakukan selama 6 minggu. Hasil penelitian menunjukkan bahwa kelompok BMKA setelah intervensi terjadi penurunan kadar glukosa darah dari 234,26 menjadi 84,78 mg/dL (p = 0,000), MDA kelompok DM dibandingkan BMKA masingmasing 2,175 dan 0,530 μmol/L (p = 0,000) serta FRAP pada kelompok DM dan BMKA masing-masing 69 dan 216 nmol/mL (p = 0,000). Kesimpulannya adalah beras merah dengan pengkayaan kappa-karagenan dan ekstrak antosianin mampu menurunkan kadar glukosa darah dan meningkatkan antioksidan plasma tikus diabetes yang ditandai dengan penurunan nilai MDA dan peningkatan nilai FRAP.

scholarly journals Potential of karamunting (Rhodomyrtus tomentosa) fraction against kidney damage in diabetic rats

2021 ◽  
Vol 67 (2) ◽  
pp. 9-16
Author(s):  
Mgs Irsan Saleh ◽  
Rachmat Hidayat ◽  
Geri Febriyanto ◽  
Nita Parisa
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Diabetic Rats ◽  
Kidney Damage ◽  
Male Rats ◽  
Native Plant ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
The Impact ◽  
Rhodomyrtus Tomentosa

Summary Introduction: Karamunting (Rhodomyrtus tomentosa) is Indonesian native plant that contains high anti-oxidant compounds with the potential to suppress oxidative stress activity in cells. This study is the first research to specifically explore the role of the karamunting fraction in protecting kidney damage due to the formation of AGEs in the renal glomerulus in diabetes mellitus. Objective: This study aims to assess the impact of karamunting on blood glucose profile, HbA1c, insulin profile and kidney condition (AGEs expression, TNF-α and kidney histology) in diabetic rats. Methods: The karamunting extraction process was carried out by maceration with ethanol. Induction of diabetes mellitus was carried out using streptozotocin. Thirty male rats (Rattus norvegicus), Wistar strain weighing between 200–250 grams were the subject in this study. HbA1c, insulin, TNF-α, AGEs levels were evaluated in the kidney tissue. Histological assessment of the kidney was carried out. Results: Karamunting can improve blood glucose levels in diabetic rats. This improvement in blood glucose levels causes an improvement in the level of HbA1c. Administration of karamunting fraction can prevent AGEs deposition in renal tissue, which in turn will inhibits the inflammation and prevents further damage of kidneys. Conclusion: Karamunting fraction can improve blood glucose regulation so that it can prevent further damage and complications of kidney organs in diabetic rats.

scholarly journals The effect of Ferulago angulata (Schelchet) Boiss on blood glucose levels and suppression of diabetes in rats

2019 ◽  
Vol 88 (3) ◽  
pp. 349-354 ◽  
Author(s):  
Rahil Parsamehr ◽  
Somayeh Bohlouli
Keyword(s):  
Diabetes Mellitus ◽  
Enzyme Activity ◽  
Blood Glucose ◽  
Intraperitoneal Injection ◽  
Diabetic Rats ◽  
Male Rats ◽  
Hydroalcoholic Extract ◽  
Glucose Levels ◽  
Liver Enzyme Activity ◽  
Blood Glucose Levels

Diabetes mellitus is a metabolic disease characterized by elevated blood glucose levels. Medicinal plant Ferulago angulata has anti-oxidant properties. The aim of the present research was to evaluate the effect of a hydroalcoholic extract of F. angulata on the blood glucose level and liver enzyme activity in diabetic male rats. Forty-eight male rats were randomly divided into eight groups, including normal and diabetic groups. Diabetes was induced by a single intraperitoneal injection of streptozotocin at 40 mg·kg-1 body weight. Rats were treated with an intraperitoneal injection of the extract for three weeks. At the end of the experimental period, fasting blood samples were collected, and blood glucose and liver enzyme activity were measured. This study demonstrated that serum glucose levels in diabetic groups treated with F. angulata were significantly lower than those in diabetic rats (P < 0.001). Alanine aminotransferase levels in diabetic rats treated with F. angulata were significantly decreased compared to diabetic rats (P < 0.001). Aspartate aminotransferase levels in diabetic rats treated with 200 and 400 mg·kg-1 F. angulata were significantly decreased compared to diabetic rats (P < 0.001). No significant differences were observed in the serum levels of blood glucose and liver enzymes in the normal group treated with different doses of the extract. These results show that the hydroalcoholic extract of F. angulata might be effective in the treatment of diabetes and consequently alleviate the liver damage caused by streptozotocin-induced diabetes mellitus. This effect might be due to the presence of flavonoids and their antioxidant features.

scholarly journals The effects of Commiphora mukul extract on spermatogenesis and testosterone levels in male diabetic rats

2020 ◽  
Vol 47 (1) ◽  
pp. 34-41
Author(s):  
Ali Akbar Rezaei ◽  
Iraj Salehi ◽  
Seyed Asaad Karimi ◽  
Mehdi Rahnama
Keyword(s):  
Sperm Count ◽  
Serum Testosterone ◽  
Diabetic Rats ◽  
Sperm Parameters ◽  
Epididymal Sperm ◽  
Testosterone Levels ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Commiphora Mukul ◽  
Male Wistar Rats

Objective: The strong antioxidant activity of <i>Commiphora mukul</i> prompted us to conduct the present study to explore whether treatment with <i>C. mukul</i> extract (CME) would have any protective influence on sperm parameters, testosterone levels, and plasma glucose levels in streptozotocin (STZ)-induced diabetic rats.Methods: Male Wistar rats were randomly divided into four groups: control, control animals treated with CME, diabetic animals, and diabetic animals treated with CME. CME extract (300 mg/kg) was administered for 60 days by daily gavage. Diabetes was induced by an intraperitoneal injection of 50 mg/kg STZ. The epididymal sperm count, weight, motility, morphology, viability, and serum testosterone and glucose levels were determined.Results: In the diabetic animals, CME decreased blood glucose levels (<i>p</i><0.05), increased the total sperm count (<i>p</i><0.05), and decreased the proportion of sperm with abnormal morphology (<i>p</i><0.05). Diabetes reduced sperm motility (<i>p</i><0.001), and CME supplementation partially reversed this effect of diabetes (<i>p</i>=0.003). Furthermore, in diabetic animals, CME decreased the proportion of immotile sperm (<i>p</i><0.001). In rats, diabetes caused a significant decrease (<i>p</i><0.05) in serum testosterone levels (F[3, 28]=3.283, <i>p</i>=0.035), but treatment of diabetic animals with CME increased serum testosterone levels.Conclusion: The present study demonstrated that C. mukul possesses proandrogenic activity and exerts a beneficial effect on sperm parameters in diabetic rats.

scholarly journals Effect of tamsulosin on blood glucose levels in euglycemic and alloxan induced diabetic rats and its interaction with glibenclamide

2018 ◽  
Vol 7 (8) ◽  
pp. 1599
Author(s):  
Sumit Satpute ◽  
Rajan Nerurkar ◽  
Manish Kokne
Keyword(s):  
Blood Glucose ◽  
Normal Saline ◽  
Repeated Measures ◽  
Diabetic Rats ◽  
Significant Rise ◽  
T Test ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Increase Blood Glucose Level ◽  
Male Wistar Rats

Background: To evaluate the effect of tamsulosin on blood glucose levels in euglycaemic rats and to investigate the effect of glibenclamide, tamsulosin and their combination on alloxan induced diabetic rats.Methods: Albino male wistar rats were randomly assigned into 6 groups (2 euglycaemic and 4 alloxan induced diabetic rats groups). In Euglycaemic rats either normal saline (0.5ml P.O) or tamsulosin (0.072mg/kg P.O) were given and blood glucose levels was estimated at 0 hr, 30min, 1hr, 2hr, 4hr on day 1 and at 0hr and 1hr on day 3 and day 7. Four groups of diabetic rats were given normal saline (0.5ml P.O), glibenclamide (5mg/kg P.O), tamsulosin (0.072mg/kg P.O), combination of glibenclamide and tamsulosin respectively and blood glucose levels were estimated on day 1, 3 and 7. Repeated measures ANOVA or paired ‘t ‘test were used for within group comparison and one way ANOVA or unpaired ‘t’ test were used for between group comparison.Results: In euglycaemic rats tamsulosin caused significant rise in blood glucose levels at 1 hr on all days and in diabetic rats tamsulosin itself did not cause any significant alteration in blood glucose levels. However, its combination with glibenclamide delayed the onset of hypoglycemic effect of glibenclamide & also reduced its hypoglycemic effect.Conclusions: Tamsulosin significantly increase blood glucose level in euglycaemic rats and it interact with Glibenclamide to reduce its hypoglycemic activity in diabetic rats.

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