Hypoglycemic properties of pectine from pumpkin (Cucurbita maxima d.) in a model of alloxan-induced type 1 diabete mellitus

2018 ◽  
pp. 82-89
Author(s):  
Т.В. Федорова ◽  
А.А. Торкова ◽  
К.В. Лисицкая ◽  
И.Б. Алчинова
Keyword(s):  
Antioxidant Properties ◽  
Diabetic Rats ◽  
Hypoglycemic Agents ◽  
Initial Symptom ◽  
Cucurbita Maxima ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Male Wistar Rats ◽  
Alloxan Monohydrate ◽  
Experimental Group

Биохимическим маркером сахарного диабета и его тяжелейших осложнений является гипергликемия. В качестве новых средств растительного происхождения, обладающих гипогликемической активностью, в настоящее время рассматривают пектины. Цель исследования - оценка функциональных свойств пектина, полученного из тыквенного жома с использованием кавитационно-мембранной технологии. Методика. Использована модель аллоксан-индуцированного сахарного диабета. Эксперимент проводили на крысах - самцах Wistar (инъекции раствора аллоксана моногидрата из расчета 43 мг/кг массы). Развитие диабета подтверждалось уровнем глюкозы (>10 мМ) в периферической крови. Для тестирования гипогликемической активности тыквенного пектина опытной группе крыс внутрижелудочно с помощью металлического зонда (Kent Scientific, США) вводили 5% раствор пектина в дистиллированной воде в дозировке 25 мг/100 г живой массы в сутки в течение 3 нед. Пектин, полученный из тыквенного жома с использованием кавитационно-мембранной технологии, имел следующие характеристики: диапазон молекулярных масс 90-120 кДа, содержание полигалактуроновой кислоты в среднем около 75% и степень этерификации 72%, что позволяет его отнести к пектинам с высокой степенью этерификации. Результаты. У животных 2-й и 3-й групп по истечении 1-3 нед. после воспроизведении диабета выявлена гипергликемия - уровень глюкозы в цельной крови был значимо выше. Статистический анализ «size effect» показал, что в начале эксперимента различия в уровне глюкозы в крови животных 2-й и 3-й групп незначительны (d = 0,39). Через 3 нед. они достигают среднего эффекта (d = 0,50). Этот факт можно расценивать как тенденцию к нормализации уровня глюкозы на фоне приема тыквенного пектина. Значимое снижение сывороточной концентрации фруктозамина в опытной группе показало гипогликемический эффект тыквенного пектина. Введение пектина животным также снижало содержание холестерина в печени и сывороточную концентрацию неэстерефицированных жирных кислот (НЭЖК), демонстрируя его гипохолестеринемические свойства. Антиоксидантные свойства пектина проявлялись нормализацией уровня ТБК-реактивных продуктов в сыворотке крови опытной группы животных. Заключение. Подтверждены гипогликемический, гипохолестеринемический и антиоксидантный эффекты пектина из тыквы (Cucurbita maxima D.) при аллоксан-индуцированном сахарном диабете. Hyperglycemia is a biochemically defined initial symptom of diabetes and its serious complications (atherosclerosis, retinopathy, kidney damage). Pectins are currently considered as novel plant-produced hypoglycemic agents. The aim of this study was to evaluate bio-functional properties of pumpkin pectin obtained from pumpkin pulp using cavitation-membrane technologies in a model of alloxan-induced diabetes. Methods. Male Wistar rats were used in the experiments. Diabetes was modeled by injections of 4.3% alloxan monohydrate solution. The development of diabetes was confirmed by glucose concentration in peripheral blood (glucose levels of >10 mM in whole blood was consistent with diabetes). To test the hypoglycemic activity of pumpkin pectin, a 5% pectin solution in distilled water was administered to rats of the experimental group (25 mg/100 g body weight, daily, for three weeks) through a metal gastric tube (Kent Scientific, USA). Results. A significant decrease of fructosamine concentration observed in the experimental group indicated a hypoglycemic effect of pectin. Administration of pectin to animals also reduced concentrations of cholesterol in liver and non-esterified fatty acids (NEFAs) in blood serum, which demonstrated cholesterol-lowering properties of pectin. Antioxidant properties of pectin provided a decrease in serum TBA-reactive products to the level observed in non-diabetic rats, in pectin-treated diabetic animals compared to the untreated diabetic group.

scholarly journals Effects of Apocynin on Heart Muscle Oxidative Stress of Rats with Experimental Diabetes: Implications for Mitochondria

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 335
Author(s):  
Estefanía Bravo-Sánchez ◽  
Donovan Peña-Montes ◽  
Sarai Sánchez-Duarte ◽  
Alfredo Saavedra-Molina ◽  
Elizabeth Sánchez-Duarte ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Insulin Sensitivity ◽  
Cardiac Tissue ◽  
Diabetic Rats ◽  
Oxidase Inhibitor ◽  
Beneficial Effects ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Transport Chain ◽  
Male Wistar Rats

Diabetes mellitus (DM) constitutes one of the public health problems today. It is characterized by hyperglycemia through a defect in the β-cells function and/or decreased insulin sensitivity. Apocynin has been tasted acting directly as an NADPH oxidase inhibitor and reactive oxygen species (ROS) scavenger, exhibiting beneficial effects against diabetic complications. Hence, the present study’s goal was to dissect the possible mechanisms by which apocynin could mediate its cardioprotective effect against DM-induced oxidative stress. Male Wistar rats were assigned into 4 groups: Control (C), control + apocynin (C+A), diabetes (D), diabetes + apocynin (D+A). DM was induced with streptozotocin. Apocynin treatment (3 mg/kg/day) was applied for 5 weeks. Treatment significantly decreased blood glucose levels and insulin resistance in diabetic rats. In cardiac tissue, ROS levels were higher, and catalase enzyme activity was reduced in the D group compared to the C group; the apocynin treatment significantly attenuated these responses. In heart mitochondria, Complexes I and II of the electron transport chain (ETC) were significantly enhanced in the D+A group. Total glutathione, the level of reduced glutathione (GSH) and the GSH/ oxidized glutathione (GSSG) ratio were increased in the D+A group. Superoxide dismutase (SOD) and the glutathione peroxidase (GSH-Px) activities were without change. Apocynin enhances glucose uptake and insulin sensitivity, preserving the antioxidant defense and mitochondrial function.

scholarly journals EVALUATION OF ANTIDIABETIC AND ANTIOXIDANT EFFECTS OF ETHANOLIC LEAF EXTRACT OF ERYTHRINA ABBYSINICA LAM, EX DC

2018 ◽  
Vol 11 (8) ◽  
pp. 300
Author(s):  
Kamadyaapa Davie Rexon ◽  
Gondwe Mavuto Masopera ◽  
Shauli Mathulo ◽  
Sewani Rusike Constance ◽  
Nkeh Chungag Benedicta
Keyword(s):  
Blood Glucose ◽  
Leaf Extract ◽  
Antioxidant Properties ◽  
Diabetic Rats ◽  
Control Group ◽  
Oral Glucose ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Ethanolic Leaf Extract ◽  
Antioxidant Effects

  Objective: This study was conducted to scientifically evaluate the antidiabetic and antioxidant effects of ethanolic leaf extract of Erythrina abbysinica (EEA).Methods: Acute and sub-chronic effects of EEA at 100, 200, and 400 mg/kg/bwt and glibenclamide (GL) at 5 mg/kg/bwt. were evaluated in both normal and streptozotocin (STZ)-induced diabetic male Wistar rats (250–300 g). The acute studies were performed using oral glucose tolerance test (OGTT). In sub-chronic studies, animals were orally administered with EEA and GL daily for 6 w. Brine shrimp assay was used to determine the toxicity of EEA. 1, 1-diphenyl-2-picrylhydrazyl, ferric reducing capacity of plasma, and thiobarbituric acid reactive substances assays were used to determine antioxidant properties of EEA.Results: Following OGTT, EEA significantly (p<0.05) and dose-dependently (100, 200, and 400 mg/kg/bwt) decreased blood glucose levels in both normal and STZ-induced diabetic rats when compared with positive and negative control counterparts at all-time points, whereas GL significantly (p<0.05) decreased blood glucose only in normal rats but not in diabetic rats. Daily, oral administration of EEA for 6 w significantly (p<0.05) and dose-dependently (100, 200, and 400 mg/kg/bwt) decreased blood glucose levels in STZ-induced diabetic rats when compared with the diabetic control group. EEA revealed weak toxicity with a lethal concentration50 value of 997 μg/ml). Furthermore, EEA showed significant free radical scavenging, total antioxidant, and anti-lipid peroxidative capacities.Conclusion: The study has shed more light on the scientific basis for the use of E. abbysinica in management of diabetes in some communities of Eastern Cape of South Africa.

scholarly journals ANTIDIABETIC EFFECT OF MIKANIA SCANDENS ON ALLOXAN-INDUCED RAT MODEL

2018 ◽  
Vol 11 (3) ◽  
pp. 109
Author(s):  
Rimashree Baishya ◽  
Anjan Adhikari ◽  
Sharmistha Biswas ◽  
Soma Banerjee
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Ethanolic Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Standard Drug ◽  
Glucose Levels ◽  
Male Wistar Rats ◽  
Histopathological Studies ◽  
Alloxan Monohydrate

 Objective: The objective was to study the evaluation of antidiabetic and antioxidant activity of different doses ethanolic extract of Mikania scandens leaves.Methods: The rats were divided into five groups of six animals each (non-diabetic control, diabetic control, standard drug-treated, and plant treated groups (250 and 500 mg/kg body weight)). Diabetes was induced in the healthy male Wistar rats (150–200 g body weight, 4–6 weeks old) by the administration of alloxan monohydrate (150 mg/kg, i.p.). On the 0th, 3th, 7th, 11th, and 15th days, the blood samples were analyzed for blood glucose. The antioxidant and antidiabetic parameters were evaluated by standard protocol. The liver tissue was used for histopathological assessment of liver damage.Results: The ethanolic extract of leaves of M. scandens showed a significant reduction (p<0.001) in the blood glucose levels and the antioxidant levels, with 500 mg/kg of body weight in the alloxan-induced diabetic rats as compared to the controls. Histopathological studies provided evidence that the leaf extract possesses antidiabetic activity.Conclusions: The ethanolic extract of leaves of M. scandens showed potent antidiabetic and antioxidant abilities against alloxan-induced rats, which indicate the presence of hypoglycemic constituents in the plant.

scholarly journals Effect Of Withania Coagulans And Liraglutide On Serum Glp-1, Postprandial And Fasting Blood Glucose In Streptozotocin Induced Diabetic Rats

2019 ◽  
Vol 09 (02) ◽  
pp. 120-123
Author(s):  
Abdul Samad ◽  
Noor Nasir Rajpoot ◽  
Hira Ayaz ◽  
Noman Sadiq
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Postprandial Blood Glucose ◽  
Controlled Study ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Withania Coagulans ◽  
Group B ◽  
Experimental Group

Objective: To evaluate effect of Withania coagulans and liraglutide on serum Glucagon like peptide-1, Postprandial and Fasting Blood Glucose levels in streptozotocin induced diabetic rat. Study Design and Setting: This randomize control trile was conducted at Islamic International Medical College in collaboration with National Institute of Health Islamabad. Methodology: This randomized controlled study was performed on a total of forty male Sprague dawly rats, which were initially divided into two groups; Group A (n=10) and Experimental Group (n=30). Diabetes in the Experimental group B was induced by intraperitoneal administration of streptozotocin for 5 days (30mg/kg/day). Diabetes was checked in experimental group by measuring fasting blood glucose (mg/dl) on day 6. Experimental group was further divided into Group B (Diabetic control), Group C (Withania coagulans-treated) and Group D (Liraglutide-treated). Blood sampling was done at day 30 and serum GLP-1, postprandial and fasting blood glucose levels were measured and compared in all groups. Results: Fasting and postprandial blood glucose levels of group C and D were significantly reduced as compared to group B. Serum GLP-1 levels were significantly increased in group C and D as compared to group B. Conclusion: Withania coagulans reduces hyperglycemia in diabetic rats through increasing GLP-1 hormone.

scholarly journals Effect of Sorghum-tigernut Ibyer (A Traditional Gruel) on the Fasting Blood Glucose Levels of Alloxan-induced Diabetic Rats

2019 ◽  
pp. 260-268
Author(s):  
Shalem Shiekuma ◽  
Moses Ukeyima ◽  
Msendoo, Janet Ahuah ◽  
Idoko Blessing ◽  
Terzungwe Tughgba
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Saline Water ◽  
Fasting Blood Glucose ◽  
Sensory Attributes ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Sorghum Flour ◽  
Blood Glucose Levels ◽  
Alloxan Monohydrate

Background: There is growing interest in the use of natural foods in the management of chronic diseases like diabetes. Ibyer is a fibre rich gruel consumed amongst the Tiv people of Benue State made from whole sorghum or millet flours. Aim: The aim of the study was to evaluate the effect of sorghum-tigernut ibyer on the fasting blood glucose levels and body weight of alloxan monohydrate-induced diabetic rats. Methods: Sorghum flour (SF) and tigernut flour (TNF) were blended at different proportions (100:00; 90:10; 80:20; 70:30) for the purpose of ibyer production. The flour samples were subjected to proximate analysis using standard analytical procedures, the sensory attributes of ibyer produced from the different flour samples was evaluated on a 9-point hedonic scale. Thirty (30) male Wistar rats (100–180 g body weight) were grouped into five (1-5) each group containing six rats. They were induced with diabetes by injecting them with 150ml/kg of body weight with alloxan monohydrate dissolved in saline water (0.9% NaCl) except for group 1. Blood samples were collected from the tail of the rats, prior to induction, 48hrs after induction and 72 hrs after three days of continuous feeding with test diet. Fasting blood glucose was measured using a standard glucometer and test strips. Results: The sensory attributes indicated that ibyer produced from the flour samples were generally acceptable. Fasting blood glucose levels after 72 hrs of feeding were found to be lowered more in groups giving flours with a higher proportion of Tigernut. Conclusion: The results indicated that sorghum-tigernut ibyer exerted hypoglycaemic effect on the experimental animals.

scholarly journals Profil Antioksidan Darah Tikus Diabetes dengan Asupan Beras Merah yang Diperkaya Kappa-Karagenan dan Ekstrak Antosianin

2017 ◽  
Vol 37 (1) ◽  
pp. 82 ◽  
Author(s):  
Nurhidajah Nurhidajah ◽  
Mary Astuti ◽  
Sardjono Sardjono ◽  
Agnes Murdiati
Keyword(s):  
Blood Glucose ◽  
Diabetic Rats ◽  
Red Rice ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Reducing Ability ◽  
Male Wistar Rats ◽  
Kappa Carrageenan ◽  
Plasma Antioxidant

This study aimed to analyze the effect of red rice enriched-kappa-carrageenan and anthocyanin extracts on blood antioxidant profile in diabetic rats. Variables analyzed in this research were blood glucose, malondialdehyde (MDA) level, and plasma antioxidant by Ferric Reducing Ability of Plasma (FRAP) method. This study was conducted in vivo on male Wistar rats aged 2.5 months using completely randomized design. Rats divided into 6 groups based on types of feed, standard feed (normal and DM), red rice (BM), red rice enriched kappa-carrageenan (BMK), red rice enriched extracts of anthocyanin (BMA) and red rice enriched with kappa-carrageenan and extract anthocyanin (BMKA). Experiments were carried out for 6 weeks. Rats feed with red rice showed decreased in blood glucose levels from 234.26 to 84.78 mg/dL (p = 0.000), MDA diabetic group compared to BMKA 2.175 and 0.530 μmol/L (p = 0.000) respectively, and the rate of FRAP in DM and BMKA 69 and 216 nmol/mL (p = 0.000) respectively. This study showed that red rice enriched with kappa-carrageenan and anthocyanin extract was able to decrease blood glucose levels and increase plasma antioxidant of diabetic rats which characterized by decreased MDA value and increased FRAP value. ABSTRAKPenelitian ini bertujuan mengkaji pengaruh pemberian beras merah yang diperkaya kappa-karagenan dan ekstrak antosianin terhadap profil antioksidan darah pada tikus Diabetes Melitus (DM). Indikator penelitian adalah penurunan glukosa darah dan angka Malondialdehid (MDA) serta peningkatan antioksidan plasma dengan metode Ferric Reducing Ability of Plasma (FRAP). Penelitian ini dilakukan secara in vivo pada hewan coba tikus Wistar usia 2,5 bulan dengan desain penelitian rancangan acak lengkap (RAL). Tikus dibagi 6 kelompok pakan, yaitu standar negatif dan positif (normal dan DM), beras merah (BM), beras merah ditambah kappa-karagenan (BMK), beras merah ditambah ekstrak antosianin (BMA), dan beras merah ditambah kappa-karagenan dan ekstrak antosianin (BMKA). Percobaan dilakukan selama 6 minggu. Hasil penelitian menunjukkan bahwa kelompok BMKA setelah intervensi terjadi penurunan kadar glukosa darah dari 234,26 menjadi 84,78 mg/dL (p = 0,000), MDA kelompok DM dibandingkan BMKA masingmasing 2,175 dan 0,530 μmol/L (p = 0,000) serta FRAP pada kelompok DM dan BMKA masing-masing 69 dan 216 nmol/mL (p = 0,000). Kesimpulannya adalah beras merah dengan pengkayaan kappa-karagenan dan ekstrak antosianin mampu menurunkan kadar glukosa darah dan meningkatkan antioksidan plasma tikus diabetes yang ditandai dengan penurunan nilai MDA dan peningkatan nilai FRAP.

scholarly journals The effects of Commiphora mukul extract on spermatogenesis and testosterone levels in male diabetic rats

2020 ◽  
Vol 47 (1) ◽  
pp. 34-41
Author(s):  
Ali Akbar Rezaei ◽  
Iraj Salehi ◽  
Seyed Asaad Karimi ◽  
Mehdi Rahnama
Keyword(s):  
Sperm Count ◽  
Serum Testosterone ◽  
Diabetic Rats ◽  
Sperm Parameters ◽  
Epididymal Sperm ◽  
Testosterone Levels ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Commiphora Mukul ◽  
Male Wistar Rats

Objective: The strong antioxidant activity of <i>Commiphora mukul</i> prompted us to conduct the present study to explore whether treatment with <i>C. mukul</i> extract (CME) would have any protective influence on sperm parameters, testosterone levels, and plasma glucose levels in streptozotocin (STZ)-induced diabetic rats.Methods: Male Wistar rats were randomly divided into four groups: control, control animals treated with CME, diabetic animals, and diabetic animals treated with CME. CME extract (300 mg/kg) was administered for 60 days by daily gavage. Diabetes was induced by an intraperitoneal injection of 50 mg/kg STZ. The epididymal sperm count, weight, motility, morphology, viability, and serum testosterone and glucose levels were determined.Results: In the diabetic animals, CME decreased blood glucose levels (<i>p</i><0.05), increased the total sperm count (<i>p</i><0.05), and decreased the proportion of sperm with abnormal morphology (<i>p</i><0.05). Diabetes reduced sperm motility (<i>p</i><0.001), and CME supplementation partially reversed this effect of diabetes (<i>p</i>=0.003). Furthermore, in diabetic animals, CME decreased the proportion of immotile sperm (<i>p</i><0.001). In rats, diabetes caused a significant decrease (<i>p</i><0.05) in serum testosterone levels (F[3, 28]=3.283, <i>p</i>=0.035), but treatment of diabetic animals with CME increased serum testosterone levels.Conclusion: The present study demonstrated that C. mukul possesses proandrogenic activity and exerts a beneficial effect on sperm parameters in diabetic rats.

scholarly journals Phytochemical compositions, and hypoglycemic effect of methanol leaf extract of Telfairia Occidentalis in alloxan-induced diabetic rats

2021 ◽  
Vol 01 (01) ◽  
pp. 52-60
Author(s):  
Asmau N. Abubakar ◽  
Fatimah O. Badmos ◽  
Abubakar N. Saidu ◽  
Ibrahim O. Yunus ◽  
Bashir Lawal
Keyword(s):  
Blood Glucose ◽  
Leaf Extract ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Hypoglycemic Effect ◽  
Appreciable Amount ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Telfairia Occidentalis ◽  
Alloxan Monohydrate

Background: The use of medicinal plants for the treatment of diabetes is increasing due to several reasons such as safety, affordability and efficacy. In the present study, the phytochemical compositions and hypoglycemic effect of the methanol leaf extract of Telfairia occidentalis (ML-TO) were investigated. Methods: Phytochemical screening was conducted using standard methods. Fifteen rats were divided into 5 groups (n=3). Diabetes was induced in rats allocated to groups A-D using alloxan monohydrate (120 mg/kg BW) and was treated with 200 and 400 mg/kg BW ML-TO, 5 mg/kg BW glibenclamide and 5 ml/kg BW normal saline respectively. All treatments were administered orally, once daily for 29 days. Results: The results revealed that the ML-TO contains phenols, alkaloids, tannins, steroids, glycosides while flavonoids and saponins were not detected. Quantitatively, total phenols (9570±13.24 µg/mg) was the most abundant phytochemicals identified while tannins (3000.56±23.45 µg/mg) and alkaloids (0.2378±0.013µg/mg) were also present in appreciable amount in ML-TO. The extract produced a significant (p <0.05) and dose-dependent hypoglycemic effect with maximum activities occurring at 400 mg/kg BW having suppressed the fasting blood glucose levels from 334±33.62 to 137±37.31 mg/dL while the rats treated with 200 mg/kg BW lowered blood glucose level from 386±171 to 174±108.55mg/dL. The standard antidiabetic drug (Glibenclamide) produced a comparable hypoglycemic effect and suppressed the FBS from 350±169.74 to125±76.35 mg/dL. Conclusion: ML-To had a significant hypoglycemic effect and was able to ameliorate the weight loss in the diabetic rats, hence it could serve as a source of potential hypoglycemic agent

scholarly journals Effect of tamsulosin on blood glucose levels in euglycemic and alloxan induced diabetic rats and its interaction with glibenclamide

2018 ◽  
Vol 7 (8) ◽  
pp. 1599
Author(s):  
Sumit Satpute ◽  
Rajan Nerurkar ◽  
Manish Kokne
Keyword(s):  
Blood Glucose ◽  
Normal Saline ◽  
Repeated Measures ◽  
Diabetic Rats ◽  
Significant Rise ◽  
T Test ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Increase Blood Glucose Level ◽  
Male Wistar Rats

Background: To evaluate the effect of tamsulosin on blood glucose levels in euglycaemic rats and to investigate the effect of glibenclamide, tamsulosin and their combination on alloxan induced diabetic rats.Methods: Albino male wistar rats were randomly assigned into 6 groups (2 euglycaemic and 4 alloxan induced diabetic rats groups). In Euglycaemic rats either normal saline (0.5ml P.O) or tamsulosin (0.072mg/kg P.O) were given and blood glucose levels was estimated at 0 hr, 30min, 1hr, 2hr, 4hr on day 1 and at 0hr and 1hr on day 3 and day 7. Four groups of diabetic rats were given normal saline (0.5ml P.O), glibenclamide (5mg/kg P.O), tamsulosin (0.072mg/kg P.O), combination of glibenclamide and tamsulosin respectively and blood glucose levels were estimated on day 1, 3 and 7. Repeated measures ANOVA or paired ‘t ‘test were used for within group comparison and one way ANOVA or unpaired ‘t’ test were used for between group comparison.Results: In euglycaemic rats tamsulosin caused significant rise in blood glucose levels at 1 hr on all days and in diabetic rats tamsulosin itself did not cause any significant alteration in blood glucose levels. However, its combination with glibenclamide delayed the onset of hypoglycemic effect of glibenclamide & also reduced its hypoglycemic effect.Conclusions: Tamsulosin significantly increase blood glucose level in euglycaemic rats and it interact with Glibenclamide to reduce its hypoglycemic activity in diabetic rats.

scholarly journals Can regular moderate exercise lead to changes in miRNA-146a and its adapter proteins in the kidney of streptozotocin-induced diabetic male rats?

2017 ◽  
Vol 51 (3) ◽  
pp. 145-152 ◽  
Author(s):  
H Oghbaei ◽  
N Ahmadi Asl ◽  
F Sheikhzadeh
Keyword(s):  
Mrna Expression ◽  
Interleukin 1 ◽  
Rna Isolation ◽  
Diabetic Rats ◽  
Male Rats ◽  
Adapter Proteins ◽  
Moderate Exercise ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Male Wistar Rats

AbstractObjective.The aim of this study was to assess whether microRNA-146a and its adapter proteins TNF receptor associated factor6 (TRAF6) and interleukin-1 receptor-associated kinase-1 (IRAK1) may be changed in the kidney of streptozotocin-induced diabetic rats, following regular moderate exercise.Methods.Forty adult male Wistar rats were allocated randomly into four groups (n=10), including sedentary control (SC), sedentary diabetic (SD), healthy sixty-day exercise (H60E), and diabetic sixty-day exercise (D60E) groups. Diabetes was induced by an intraperitoneal injection of 60 mg/kg streptozotocin. After 48 h, blood glucose levels >250 mg/dl was included to diabetic rats. After 2 days of diabetes induction, the exercise protocol began. Animals were exposed to 5 days of consecutive treadmill exercise for 60 min/day with the 22 m/min speed for 60 days. The kidneys of the rats were removed and microRNA was extracted from them using the miRCURYTM RNA isolation kit.Results.In diabetic rats, statistical analysis revealed a significant decrease in miR-146a expression, non-significant decrease in IRAK1 mRNA expression, and non-significant increase in TRAF6 and NF-kB mRNA expression compared to the SC group. Exercise led to a non-significant increase in the expression of miR-146a and NF-kB mRNA in the kidneys of the diabetic group as compared to the SD group, significant increase in TRAF6 and IRAK1 mRNA expression compared to the H60E group, and significant increase in TRAF6 mRNA expression compared to the SD group.Conclusion.The present data indicate that exercise might be able to help in the prevention in the diabetic nephropathy development.

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