Acetone-water mixture is a competent solvent to extract phenolics and antioxidants from four organs of Eucalyptus camaldulensis

2019 ◽  
Vol 44 (3) ◽  
pp. 231-239
Author(s):  
Alyaa Nasr ◽  
Xinxin Zhou ◽  
Tingting Liu ◽  
Jing Yang ◽  
Guo-Ping Zhu
Keyword(s):  
Phenolic Compounds ◽  
Biological Activities ◽  
Great Influence ◽  
Eucalyptus Camaldulensis ◽  
Total Phenolic ◽  
Water Mixture ◽  
Plant Seed ◽  
Acetone Water ◽  
Phenolic Constituents ◽  
Wide Range

Abstract Objective Eucalyptus camaldulensis is recognized to be one of the major introduced eucalypts, the plant extracts possess a wide range of phytochemicals, which are accountable for its antioxidant and pharmacological activities. The extraction efficiency of such chemical compounds is influenced by the applied extraction conditions. To test the effect of solvent type on the yield of phenolics and antioxidants from E. camaldulensis, seeking for an optimization of the extraction process. Method Dry powders of four plant organs were extracted by some organic solvents besides water. The total phenolic content was detected in the crude extracts of leaf, bud, empty capsule and seed, and was further characterized by GC-MS. Results Acetone-water mixtures yielded high amounts of phenolic compounds with parallel antioxidant activities, correlation coefficients were significant (0.696–0.998). Bud and capsule are first accredited for their antioxidant potentials. The GC-MS analysis revealed the abundance of most detected phenolic constituents in the plant seed. Discussion Acetone-water is a successful and appropriate mixture for the extraction of phenolic compounds from E. camaldulensis. It could give better yields and enhance the biological activities of its corresponding extracts. Conclusion The accurate choice of an extraction solvent has a great influence on the yields of phenolics.

Comparison Among Five Eucalyptus Species Based on Their Leaf Contents of Some Primary and Secondary Metabolites

2019 ◽  
Vol 20 (7) ◽  
pp. 573-587
Author(s):  
Alyaa Nasr ◽  
Tehmina Saleem Khan ◽  
Shi-Ping Huang ◽  
Bin Wen ◽  
Jian-Wen Shao ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Protocatechuic Acid ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Renewable Resource ◽  
Total Phenolic ◽  
Eucalyptus Species ◽  
Primary And Secondary Metabolites ◽  
Acetone Water ◽  
Phenolic Constituents

Background: Eucalyptus belongs to the Myrtaceae family. It is the most planted hardwood forest crop worldwide, representing a global renewable resource of fiber, pharmaceuticals and energy. Objective: To compare the five species, E. maidenii, E. robusta, E. citriodora, E. tereticornis and E. camaldulensis, seeking for the richest source of nutrients and pharmaceuticals. Methodology: Eucalyptus samples were subjected to some chemical determinations for both primary and secondary metabolites to verify their nutritional and pharmaceutical importance related to different extracts. GC-MS analysis was applied to detect the presence of some individual phenolic constituents in their leaves. Results: E. robusta recorded the maximum contents of carbohydrates (40.07%) and protein (31.91%). While E. camaldulensis contained the highest contents of total phenolic compounds (46.56 mg/g), tannins (40.01 mg/g) and antioxidant activities assayed by the phosphomolybednum method (57.60 mg/g), followed by E. citridora. However, E. tereticornis exhibited the highest reducing power ability (151.23 mg/g). The GC-MS highlighted 20 phenolic constituents and antioxidants which varied in their abundance in Eucalyptus leaves, 8 individual phenolics (hydroquinone, hesperitin, pyrogallol, resorcinol, protocatechuic acid, naringenin, chlorogenic acid and catechin) were maximally recorded with E. camaldulensis and secondly, with E. citridora in case of at least 5 components. Nevertheless, gallic and quinic acids were more abundant in the leaves of E. tereticornis, which may explain its high corresponding reducing powers. Conclusion: Acetone-water combination has enhanced phenolics extraction from Eucalyptus tissues. This is the first report aiming to compare between the aforementioned Eucalyptus species highlighting either their nutritional or medicinal importance.

scholarly journals Impact of Foliar Application of Amino Acids on Total Phenols, Phenolic Acids Content of Different Mints Varieties under the Field Condition

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 599
Author(s):  
Živilė Tarasevičienė ◽  
Aloyzas Velička ◽  
Aurelija Paulauskienė
Keyword(s):  
Amino Acids ◽  
Phenolic Compounds ◽  
Phenolic Acids ◽  
Foliar Application ◽  
Mentha Piperita ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Individual Response ◽  
Total Phenols ◽  
Wide Range

Phenolic compounds have a number of benefits to human health and can be used as preventive compounds for the development of some chronic diseases. Mentha plants are not only a good source of essential oils, but also contain significant levels of wide range of phenolic compounds. The aim of this research was to investigate the possibility to increase phenols content in Mentha plants under the foliar application with L-phenylalanine, L-tryptophan, L-tyrosine at two concentrations (100 mg L−1 and 200 mg L−1) and to create preconditions for using this plant for even more diverse purposes. Quantitative and qualitative analyses of phenols in mints were performed by HPLC method. Foliar application of amino acids increased the total phenol content from 1.22 to 3.51 times depending on the treatment and mint variety. The most pronounced foliar application to total phenols content was tryptophane especially in Mentha piperita “Swiss”. Mentha piperita “Swiss” was affected most by foliar application and the amount of total phenolic acids depending on the treatment ranged from 159.25 to 664.03 mg 100 g−1 (DW), respectively, non-sprayed and sprayed with tryptophane 100 mg L−1. Our results suggest that the biophenol content varies according to such factors as foliar application and variety, and every single mint variety has individual response to different applications of amino acids.

scholarly journals Plant Growth Modulates Metabolites and Biological Activities in Retama raetam (Forssk.) Webb

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2177 ◽  
Author(s):  
Mariem Saada ◽  
Hanen Falleh ◽  
Marcelo Catarino ◽  
Susana Cardoso ◽  
Riadh Ksouri
Keyword(s):  
Phenolic Compounds ◽  
Plant Growth ◽  
Biological Activities ◽  
Reducing Power ◽  
Vegetative Stage ◽  
Total Phenolic ◽  
Human Pathogens ◽  
Retama Raetam ◽  
Physiological Stage ◽  
Main Class

This work focuses on the variability of Retama raetam (Forssk.) Webb bioactive compounds as a function of the plant cycle. The main results showed that it exhibited the highest percentage of polyunsaturated fatty acids, along with superior levels of vitamin C and total phenolic compounds (66.49%, 645.6 mg·100 g−1 FW and 23.9 mg GAE·g−1, respectively) at the vegetative stage. Instead, at the flowering and mature fruiting stages, R. raetam (Forssk.) Webb exhibited notable contents of proline (25.4 μmol·g−1 DW) and carotenoids (27.2 μg·g−1 FW), respectively. The gathered data concerning the antioxidant activity highlighted the effectiveness of the vegetative stage in comparison to the other periods. Actually, IC50 and EC50 values of the hydromethanolic extract obtained from the plant shoots at the vegetative stage were of 23, 380, 410, 1160 and 960 μg·mL−1 (DPPH• and ABTS•+ radicals scavenging activity, reducing power, chelating power and β-carotene bleaching activity, respectively). Furthermore, the four studied stages showed appreciable antibacterial capacities against human pathogens with a higher efficiency of the vegetative stage extract. Finally, the LC-DAD-ESI/MSn analysis revealed the predominance of isoflavonoids as main class of phenolic compounds and demonstrates that individual phenolic biosynthesis was clearly different as a function of plant growth. These findings highlight that reaching the optimum efficiency of R. raetam (Forssk.) Webb is closely linked to the physiological stage.

scholarly journals Effect of physicochemical properties and phenolic compounds of bifloral propolis on antioxidant and antimicrobial capacity

Nova Scientia ◽  
2020 ◽  
Vol 12 (24) ◽  
Author(s):  
Rey David Vargas Sánchez ◽  
Evelin Martínez Benavidez ◽  
Javier Hernández ◽  
Gastón Ramón Torrescano Urrutia ◽  
Armida Sánchez Escalante
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Phenolic Compounds ◽  
Physicochemical Properties ◽  
Phenolic Content ◽  
Biological Activities ◽  
Sensory Characteristics ◽  
Total Phenolic ◽  
Gram Positive ◽  
Phenolic Components

In this study the effect of pollen source (mesquite and catclaw) on the sensory characteristics (appearance, color, aroma, taste, consistency and visible impurities), and physicochemical properties of raw propolis, and the phenolic content and biological activities of propolis extracts (PEs) was determined. The phenolic composition of PEs was determined by the total phenolic (TPC), flavone and flavonol (FFC), and flavanone and dihydroflavonol content (FDC). The individual phenolic components were analyzed by HPLC-DAD. The antioxidant activity was determined by the ferric-reducing power (FRAP) and free-radical scavenging activity (FRS). The antibacterial activity was evaluated against Gram-positive (Staphylococcus aureus and Listeria innocua) and Gram-negative (Echerichia coli and Salmonella thyphimurium) bacteria. The results showed that sensory characteristic and physicochemical properties of mesquite and catclaw propolis complied with international quality regulations. Fifteen phenolic compounds were identified, of which pinocembrin, naringenin, galangin, chrysin and quercetin were found a high concentration (> 3 mg/g). Mesquite propolis had the highest phenolic content (TFC and FDC), as well as antioxidant activity (> 2.5 mg Fe (II) equivalent/g; > 40% of DPPH radical inhibition) and antibacterial activity against Gram-positive bacterias in the order S. aureus > L. innocua (> 50% of inhibition for both bacterias at 500 µg/mL). These results indicating that pollen source affect the sensory characteristics and physicochemical properties of propolis, as well as the biological activity of their extracts.

Antioxidant and Antimicrobial Activities of the Fresh aerial parts of Ammi visnaga L. from Algeria

2016 ◽  
Vol 6 (2) ◽  
pp. 70-76
Author(s):  
Soumia Keddari ◽  
Narimen Benaoum ◽  
Yasmina Mokhtaria Boufadi ◽  
Mansouria Belhocine ◽  
Ali Riazi
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Phenolic Compounds ◽  
Biological Activities ◽  
Antibacterial Properties ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
E Coli ◽  
Ammi Visnaga ◽  
Wide Range

Medicinal plants have been used for countries as cures for human diseases because they contain components of therapeutic value. Among these medi-cinal plants, Ammi visnage which have an immense reservoir of potential compounds attributed to the secondary metabolites which have the advan-tage of being of great diversity of chemical structure and have a very wide range of biological activities. The objectives of the present work were to stu-dy the antioxidant and antimicrobial activity of phenolic compounds ex-tracted from A. visnaga L. Its extraction is performed by two methods, etha-nol extraction and water extraction. The results showed that A. visnaga L.. ethanolic extract contains a mixture of phytochemical classes as polyphenol, flavonoids and revealed that this plant has high antioxidant activity (IC50 0.069 mg/ml). Regarding the antimicrobial activity results expressed by the diameter of the inhibition zones by diffusion method AWDT, the most signifi-cant inhibition was observed against to Staphylococcus aureus (12 mm) to the ethanol extract at concentration of 100mg / ml. Thus the aqueous ex-tract had a significant inhibitory activity against on the strains Staphylococ-cus aureus (8 mm), E. coli ATCC 10536 (8 mm) to a concentration of 100 mg / ml. The results for the antibacterial properties have shown that Gram-positive bacteria (Staphylococcus aureus, Listeria monocytogenes and M. luteus.) were more sensitive than gram-negative (Pseudomonas aeruginosa, E. coli ATCC 10536) against from the action of phenolic compounds of the Ammi visnaga ethanolic extract.

scholarly journals Bioactive Compounds of Selected Fruit Juices

2009 ◽  
Vol 4 (5) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Aneta Wojdyłto ◽  
Jan Oszmiański
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Fruit Juice ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Sea Buckthorn ◽  
Quercetin Derivatives ◽  
Reducing Ability ◽  
Wide Range

Fruits are one of the most important sources of phenolic compounds in our diet. Many of these compounds exhibit a wide range of biological activities, especially antioxidant activity. The objective of this study was to determine the composition and antioxidant activity of phenolic compounds found in fruit juice obtained from buckthorn, flowering quince, rowanberry and hawthorn. Hydroxycinnamic acids (neochlorogenic and chlorogenic acid) and procyanidins were the main phenolics in rowanberry juice. The highest level of flavonols (quercetin derivatives) was found in sea buckthorn. All juices showed strong free-radical scavenging activity against ABTS•+ radicals and ferric reducing ability measured by the FRAP method. The strongest antioxidant activity was found in rowanberry juice, but the lowest was measured in hawthorn and sea buckthorn juices.

scholarly journals UPLC-MS/MS characterization of phenolic constituents in fennel seed extracts (Foeniculum vulgare Mill.)

2020 ◽  
Vol 79 (OCE2) ◽  
Author(s):  
Zoran Zorić ◽  
Maja Repajić ◽  
Valentina Kruk ◽  
Branka Levaj ◽  
Danijela Bursać Kovačević ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Extraction Procedure ◽  
Extraction Process ◽  
Flavonoid Glycosides ◽  
Foeniculum Vulgare ◽  
Cycle Number ◽  
Methanol Extracts ◽  
Phenolic Constituents ◽  
Wide Range ◽  
Pharmaceutical Industries

AbstractReports in the recent literature indicate that consumption of phytochemicals (e.g. plant polyphenols) has been associated with a wide range of health benefits. Fennel (Foeniculum vulgare Mill.) is common Mediterranean plant that has been widely used as medicinal and aromatic herb. Numerous studies reported the use of fennel extracts (plant or seed) due to its diversity of phenolic compounds to whom inhibitory effects against several diseases are prescribed. Hence, phenolic compounds isolated from fennel are of the great interest for the use in food, chemical and pharmaceutical industries.In order to obtain the highest quality of the fennel extracts, the selection of an effective extraction procedure is required. Nowadays, innovative extraction techniques have been introduced for isolation of bioactive compounds from plant material, among which pressurized liquid extraction (PLE) was reported as automated extraction procedure that combines effects of elevated temperature and pressure to increase the efficiency of the extraction process. Improved efficiency is also achieved by selecting the right solvent and by optimizing processing conditions (temperature, static time, cycle number).Thus, PLE was applied for the extraction of phenolic compounds from fennel seeds. The effects of exhaustive extraction (acetone followed by methanol) as well as extraction temperatures (75 and 100 °C), static time (5 and 10 min) and cycle number (1,2,3) on the phenolic compounds concentrations were evaluated. A total of 27 phenolic compounds were identified and characterized by UPLC-ESI-MS/MS whereas 6 different categories are distinguished: (1) simple phenolic acid derivatives, (2) hydroxycinnamoylquinic acid derivatives, (3) flavonoid diglycosides, (4) flavonoid O-rhamnoglucosides, (5) flavonoid glucuronides and (6) flavonoid glycosides. Phenolic compounds were identified by comparing retention time and m/z values obtained by MS and MS/MS with the mass spectra from corresponding standards whereas the quantification was made on the basis of their peak areas and comparison with calibration curve obtained with the corresponding standards. Obtained results indicated that hydroxycinnamoylquinic acid derivatives (10 compounds) and flavonoid glucuronides (3 compounds) were found as the most abundant phenolics in acetone and methanol extracts, respectively. Statistical analysis showed that optimized PLE conditions for the most effective extraction of phenolic compounds were as follows: 100 °C/10 min/3 cycles for acetone and 100 °C/5 min/3 cycles for methanol extracts. In conclusion, PLE might be considered as a rapid and effective extraction procedure that could be useful in production of fennel seeds extracts.

scholarly journals Phenolic Compounds from An Algerian Endemic Species of Hypochaeris laevigata var. hipponensis and Investigation of Antioxidant Activities

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 514 ◽  
Author(s):  
Nabila Souilah ◽  
Zain Ullah ◽  
Hamdi Bendif ◽  
Kamel Medjroubi ◽  
Tahar Hazmoune ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Quinic Acid ◽  
Total Phenolic ◽  
Antioxidant Action ◽  
Endemic Plant ◽  
Aerial Parts ◽  
Phenolic Constituents ◽  
First Time ◽  
Phenolic And Flavonoid

Hypochaeris laevigata var. hipponensis (Asteraceae) is an endemic plant from Algeria. In the current study, we analyzed for the first time its chemical composition, especially phenolic constituents of dichloromethane (DCM), ethyl acetate (EA), and n-butanol (BuOH) fractionsof the aerial parts of Hypochaeris laevigata var. hipponensis by liquid chromatography-mass spectrometry (LC-MS/MS). The number of phenolic compounds detected in DCM, EA, and BuOH fractions were found to be 9, 20, and 15, respectively. More specifically, 12 phenolic acids were detected. Among them, quinic acid, chlorogenic acid, and caffeic acid were the most abundant ones. Meanwhile, only seven flavonoids were detected. Among them, rutin, apigetrin, and isoquercitrin were the major ones. We also determined the total phenolic and flavonoid contents, and fraction EA showed the highest values, followed by BuOH, and DCM fractions. Furthermore, the antioxidant action was dictated by five methods and the tested plant fractions demonstrated a noteworthy antioxidant action.

scholarly journals Microbiological and Antioxidant Activity of Phenolic Compounds in Olive Leaf Extract

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 5946
Author(s):  
Dragana Borjan ◽  
Maja Leitgeb ◽  
Željko Knez ◽  
Maša Knez Hrnčič
Keyword(s):  
Phenolic Compounds ◽  
Leaf Extract ◽  
Biological Activities ◽  
Olive Leaf ◽  
Olive Leaves ◽  
Olive Leaf Extract ◽  
Antimicrobial Potential ◽  
Isolation And Characterization ◽  
Wide Range

According to many reports, phenolic compounds isolated from olive leaves have very good biological activities, especially antimicrobial. Presently, the resistance of microorganisms to antibiotics is greater than ever. Therefore, there are numerous recent papers about alternative solutions for inhibiting their influence on human health. Olive leaf is studied as an important source of antimicrobials with low cost and used in medicine. Numerous publications on involving green technologies for isolation of active compounds from olive leaves have appeared over the past few decades. The present review reports on current knowledge of the most isolated phenolic compounds from olive leaf extract as well as methods for their isolation and characterization. This paper uses recent research findings with a wide range of study models to describe the antimicrobial potential of phenolic compounds. It also describes the vast range of information about methods for determination of antimicrobial potential focusing on effects on different microbes. Additionally, it serves to highlight the role of olive leaf extract as an antioxidants and presents methods for determination of antioxidant potential. Furthermore, it provides an overview of presence of enzymes. The significance of olive leaves as industrial and agricultural waste is emphasized by means of explaining their availability, therapeutic and nutritional effects, and research conducted on this field.

scholarly journals Comparison of Bioactive Phenolic Compounds and Antioxidant Activities of Different Parts of Taraxacum mongolicum

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3260 ◽  
Author(s):  
Li Duan ◽  
Chenmeng Zhang ◽  
Yang Zhao ◽  
Yanzhong Chang ◽  
Long Guo
Keyword(s):  
Phenolic Compounds ◽  
Caffeic Acid ◽  
Antioxidant Activities ◽  
Pearson Correlation ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Caftaric Acid ◽  
Phenolic Constituents ◽  
Leaves And Roots ◽  
Different Parts

Herbs derived from Taraxacum genus have been used as traditional medicines and food supplements in China for hundreds of years. Taraxacum mongolicum is a famous traditional Chinese medicine derived from Taraxacum genus for the treatment of inflammatory disorders and viral infectious diseases. In the present study, the bioactive phenolic chemical profiles and antioxidant activities of flowers, leaves, and roots of Taraxacum mongolicum were investigated. Firstly, a high performance liquid chromatography method combined with segmental monitoring strategy was employed to simultaneously determine six bioactive phenolic compounds in Taraxacum mongolicum samples. Moreover, multivariate statistical analysis, including hierarchical clustering analysis, principal component analysis, and partial least squares discriminant analysis were performed to compare and discriminate different parts of Taraxacum mongolicum based on the quantitative data. The results showed that three phenolic compounds, caftaric acid, caffeic acid, and luteolin, could be regarded as chemical markers for the differences of flowers, leaves, and roots of Taraxacum mongolicum. In parallel, total phenolic contents, total flavonoid contents and antioxidant activities of different parts of Taraxacum mongolicum were also evaluated and compared. It is clear that Taraxacum mongolicum had antioxidant properties, and the antioxidant capacities of different parts of Taraxacum mongolicum in three antioxidant assays showed a similar tendency: Flowers ≈ leaves > roots, which revealed a positive relationship with their total phenolic and flavonoid contents. Furthermore, to find the potential antioxidant components of Taraxacum mongolicum, the latent relationships of the six bioactive phenolic compounds and antioxidant activities of Taraxacum mongolicum were investigated by Pearson correlation analysis. The results indicated caftaric acid and caffeic acid could be the potential antioxidant ingredients of Taraxacum mongolicum. The present work may facilitate better understanding of differences of bioactive phenolic constituents and antioxidant activities of different parts of Taraxacum mongolicum and provide useful information for utilization of this herbal medicine.

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