Chemical Composition and Biological Activity of Propolis from Brazilian Meliponinae

2000 ◽  
Vol 55 (9-10) ◽  
pp. 785-789 ◽  
Author(s):  
Milena Velikova ◽  
Vassya Bankova ◽  
Maria C. Marcucci ◽  
Iva Tsvetkova ◽  
Atanas Kujumgiev
Keyword(s):  
Biological Activity ◽  
Chemical Composition ◽  
Stingless Bees ◽  
Geographical Location ◽  
Artemia Salina ◽  
Cytotoxic Activities ◽  
Significant Activity ◽  
High Concentration ◽  
E Coli ◽  
High Cytotoxic Activity

Abstract Twenty-one propolis samples produced by 12 different Meliponinae species were analyzed by GC-MS. Several chemical types of stingless bees’ propolis could be grouped, according to the prevailing type of compounds like: “gallic acid”, “diterpenic” and “triterpenic” types. The results confirm that neither the bee species nor the geographical location determine the chemical composition of Meliponinae propolis and the choice of its plant source, respectively. This could be explained by the fact that Meliponinae forage over short distances (maximum 500 m) and thus use as propolis source the first plant exudate they encounter during their flights. The antibacterial, antifungal and cytotoxic activities of the samples were also investigated. Most samples had weak or no activity against E. coli, weak action against Candida albicans. Some of them showed significant activity against St. aureus., presumably connected to the high concentration of diterpenic acids. Samples rich in diterpenic acids possessed also high cytotoxic activity (Artemia salina test).

Synthesis and in vitro Biological Activity of New 4,6-Disubstituted 3(2H)-Pyridazinone-acetohydrazide Derivatives

2012 ◽  
Vol 67 (5-6) ◽  
pp. 257-265
Author(s):  
Murat Sukuroglu ◽  
Tijen Onkol ◽  
Fatma Kaynak Onurdağ ◽  
Gulsen Akalın ◽  
M. Fethi Şahin
Keyword(s):  
Biological Activity ◽  
Antimicrobial Activities ◽  
Cytotoxic Activities ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
E Coli ◽  
Preliminary Results ◽  
H Nmr ◽  
Pyridazinone Derivatives

New 3(2H)-pyridazinone derivatives containing a N’-benzyliden-acetohydrazide moiety at position 2 were synthesized. The structures of these newly synthesized compounds were confi rmed by IR, 1H NMR, and MS data. These compounds were tested for their antibacterial, antifungal, antimycobacterial, and cytotoxic activities. The compounds 2-[4-(4-chlorophenyl)- 6-(morpholin-4-yl)-3-oxo-(2H)-pyridazin-2-yl]-N’-(4-tert-butylbenzyliden)acetohydrazide and 2-[4-(4-chlorophenyl)-6-(morpholin-4-yl)-3-oxo-(2H)-pyridazin-2-yl]-N’-(4-chlorobenzyliden) acetohydrazide exhibited activity against both Gram-positive and Gram-negative bacteria. Most of the compounds were active against E. coli ATCC 35218. The preliminary results of this study revealed that some target compounds exhibited promising antimicrobial activities

scholarly journals Chemical Composition and Biological Activities of the Black Sea Algae Polysiphonia denudata (Dillw.) Kutz. and Polysiphonia denudata f. fragilis (Sperk) Woronich

2001 ◽  
Vol 56 (11-12) ◽  
pp. 1008-1014 ◽  
Author(s):  
Salvatore De Rosa ◽  
Zornitsa Kamenarska ◽  
Vassya Bankova ◽  
Kamen Stefanov ◽  
Stefka Dimitrova-Konaklieva ◽  
...  
Keyword(s):  
Biological Activity ◽  
Chemical Composition ◽  
Black Sea ◽  
Biological Activities ◽  
Artemia Salina ◽  
Sterol Composition ◽  
The Black Sea ◽  
Polar Components

Abstract The two investigated algae had almost identical sterol composition, but there were signifi­cant differences in the com position of the polar components and especially in the composi­tion of the volatiles. P. denudata f. fragilis extracts possessed a stronger biological activity (antibacterial, antifungal and toxicity against Artemia salina). Despite the minute morpholog­ical differences between the two algae, we recommend P. denudata f. fragilis to be regarded as P. denudata subsp. fragilis.

scholarly journals Larvicidal evaluation of the Origanum majorana L. Essential Oil against the larvae of the Aedes aegypti mosquito

2019 ◽  
Author(s):  
Renata do Socorro Barbosa Chaves ◽  
Rosany Lopes Martins ◽  
Alex Bruno Lobato Rodrigues ◽  
Érica de Menezes Rabelo ◽  
Ana Luzia Ferreira Farias ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Artemia Salina ◽  
High Cytotoxic Activity ◽  
Origanum Majorana ◽  
Main Component

ABSTRACTThis study evaluated the larvicidal activity of O. majorana essential oil, identified the chemical composition, evaluated the antimicrobial, cytotoxic and antioxidant potential. The larvicidal activity was evaluated against larvae of the third stage of Aedes aegypti, whereas the chemical composition was identified by gas chromatography coupled to mass spectrometer, the antimicrobial activity was carried out against the bacteria Pseudomonas aeruginosa, Escherichia coli and Staphylococcus auereus, the antioxidant activity was evaluated from of 2.2-diphenyl-1-picryl-hydrazila sequestration and Artemia salina cytotoxicity. Regarding to the results, the larvicidal activity showed that O. majorana essential oil caused high mortality in A. aegypti larvae. In the chromatographic analysis, the main component found in O. majorana essential oil was pulegone (57.05%), followed by the other components verbenone (16.92%), trans-p-menthan-2-one (8.57%), iso-menthone (5.58%), piperitone (2.83%), 3-octanol (2.35%) and isopulegol (1.47%). The antimicrobial activity showed that E. coli and P. aeruginosa bacteria were more sensitive to oil than S. aureus, which was resistant at all concentrations. Essential oil did not present antioxidant activity, but it has high cytotoxic activity against A. salina.

scholarly journals Chemical Composition and Biological Activity of Pulicaria vulgaris Essential Oil from Iran

2014 ◽  
Vol 9 (11) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Javad Sharifi-Rad ◽  
Abdolhossein Miri ◽  
Seyedeh Mahsan Hoseini-Alfatemi ◽  
Majid Sharifi-Rad ◽  
William N. Setzer ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Cell Lines ◽  
Chemotherapeutic Agents ◽  
Cytotoxic Activities ◽  
Hep G2 ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Aerial Parts ◽  
Mcf 7

The present study investigated the chemical composition of the essential oil (EO) from aerial parts (flowering stage) of Pulicaria vulgaris Gaertn. by GC–MS. Also, the antimicrobial activity of the EO against Gram-positive bacteria ( Bacillus cereus and Staphylococcus aureus), Gram-negative bacteria ( Escherichia coli and Pseudomonas aeruginosa) and fungi ( Aspergillus niger and Candida albicans) was tested. In total, 23 compounds were recognized, accounting for 98.08 % of the EO. The main compounds in the EO were thymol (50.22%), p-menth-6-en-2-one (carvotanacetone, 20.2%), thymol isobutyrate (16.88%), menthan-2-one (4.31%), 1-methyl-1,2-propanedione (4.13%), 2,5-dimethoxy- p-cymene (4.01%), myrtenol (1.22%), linalool (1.1%), and β-myrcene (1.9%). Results of antibacterial test of P. vulgaris essential oil showed that all assayed concentrations significantly inhibited the growth of B. cereus, S. aureus, E. coli, and P. aeruginosa at P < 0.05. MIC for B. cereus, S. aureus, E. coli, P. aeruginosa was 17.5, 25.2, 19.4 and 33.2 μg/mL respectively; antifungal screening of the essential oil of P. vulgaris showed that the oil significantly inhibited the growth of A. niger and C. albicans (MIC = 15.5 and 9.9 μg/mL, respectively). Results of cytotoxicity assay showed that the essential oil exhibited a significant cytotoxic activity against both cell lines. In case of MCF-7 and Hep-G2 cell lines, IC50 of the essential oil were 5.36 and 7.16 μμg/ml, respectively. The potent antimicrobial and cytotoxic activities of the EO may be attributed to its high contents of thymol, carvotanacetone and thymol isobutyrate. Antimicrobial and antitumor chemotherapies are showing diminishing effectiveness because of emergence of drug-resistance. Hence, using efficient natural chemotherapeutic agents such as Pulicaria vulgaris essential oil with fewer side effects is an encouraging approach to fight cancer and infectious diseases in medicine, agriculture, food science and related fields.

scholarly journals Preliminary Biological Activity Screening of Plectranthus spp. Extracts for the Search of Anticancer Lead Molecules

2021 ◽  
Vol 14 (5) ◽  
pp. 402
Author(s):  
Epole Ntungwe ◽  
Eva María Domínguez-Martín ◽  
Catarina Teodósio ◽  
Silvia Teixidó-Trujillo ◽  
Natalia Armas Capote ◽  
...  
Keyword(s):  
Biological Activity ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Human Colon ◽  
Artemia Salina ◽  
Significant Activity ◽  
Ultrasound Assisted Extraction ◽  
Microdilution Method ◽  
Ic50 Value ◽  
Dpph Method

Plectranthus species (Lamiaceae) have been employed in traditional medicine and this is now validated by the presence of bioactive abietane-type diterpenoids. Herein, sixteen Plectranthus acetonic extracts were prepared by ultrasound-assisted extraction and their biological activity was screened. The antimicrobial activity of each extract was screened against yeasts, and Gram-positive and Gram-negative bacteria. The P. hadiensis and P. mutabilis extracts possessed significant activity against Staphylococcus aureus and Candida albicans (microdilution method). Moreover, all extracts showed antioxidant activity using the DPPH method, with P. hadiensis and P. mutabilis extracts having the highest scavenging activities. Selected by the Artemia salina model, P. hadiensis and P.ciliatus possessed low micromolar anti-proliferative activities in human colon, breast, and lung cancer cell lines. Furthermore, the most bioactive extract of P. hadiensis leaves and the known abietane diterpene, 7α-acetoxy-6β-hydroxyroyleanone isolated from this plant, were tested against the aggressive type triple negative breast cancer (MDA-MB-231S). P. hadiensis extract reduced the viability of MDA-MB-231S cancer cell line cells, showing an IC50 value of 25.6 µg/mL. The IC50 value of 7α-acetoxy-6β-hydroxyroyleanone was 5.5 µM (2.15 µg/mL), suggesting that this lead molecule is a potential starting tool for the development of anti-cancer drugs.

scholarly journals Chemical profile and antimicrobial potential of essential oils of Cymbopogon citratus (DC.) Stapf, Ocimum basilicum Linn and Aniba rosaeodora Ducke

2020 ◽  
Vol 16 (6) ◽  
Author(s):  
Gustavo Oliveira Everton ◽  
Paulo Sérgio Santos Júnior ◽  
Rebecca Jemima Pereira Araújo ◽  
Aline Medeiro Ferreira ◽  
Paulo Roberto Barros Gomes ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Physicochemical Parameters ◽  
Artemia Salina ◽  
Chemical Profile ◽  
E Coli ◽  
Diffusion Technique ◽  
Mueller Hinton ◽  
Major Chemical

The study evaluated the chemical profile, antimicrobial activity and toxicity of essential oils (EOs) of C. citratus, O. basilicum and A. rosaeodora. The EOs were extracted by hydrodistillation, the chemical profile was determined using the physicochemical parameters and the chemical composition was obtained by gas chromatography coupled to mass spectrometry (GC-MS). The toxicity assay followed the Artemia salina Leach bioassay. To perform the antimicrobial activity, the Disc Diffusion technique and the Mueller Hinton Broth Dilution (MH) technique were used. The physicochemical parameters of the EOs showed satisfactory results. The major chemical composition of the EO of A. rosaeodora presented linalool (93.60%), the EO of C. citratus exhibited geranial (43.96%) and EO of O. basilicum found methyl chavicol. All EOs in this study had LC50 between 582 mg L-1 and 282 mg L-1. The antimicrobial activity of EO C. citratus demonstrated inhibition halos for S. aureus of 25 mm and 25 mm for E. coli, EO of O. basilicum exhibited inhibition halo of 18 mm for E.coli and 20 mm for S. aureus and EO of A. rosaeodora presented 11 mm for E. coli and 15 for S. aureus. As the values of MIC and MBC found, the EO of O. basilicum presented a more effective inhibitory action against S. aureus. It was concluded that the observed biological potentials encourage the application potentials in the efficiency of the control of pathogenic microorganisms.

scholarly journals THE COMPONENT COMPOSITION OF THE TRAGOPOGON ORIENTALIS VOLATILE CONSTITUENTS AND ITS BIOLOGICAL ACTIVITY

2019 ◽  
pp. 103-108
Author(s):  
Yerlan Melsuly Suleimen ◽  
Gulsana Galimzhankyzy Sissengaliyeva ◽  
Margarita Yulaevna Ishmuratova ◽  
Roza Ilemisovna Jalmakhanbetova
Keyword(s):  
Biological Activity ◽  
Chemical Composition ◽  
Cytotoxic Activity ◽  
Antiradical Activity ◽  
Component Composition ◽  
Artemia Salina ◽  
Raw Material ◽  
Plant Leaves ◽  
Volatile Constituents ◽  
Standard Drug

The purpose of this study is the chemical investigation of the composition of Tragopogon orientalis volatile constituents (VC) and studying of its biological activity. This article presents the results of research on the study of the chemical composition of species Tragopogon, as well as data of antiradical and cytotoxic activity. To study the dried and crushed aboveground part of the plant (leaves, flowers and stems) was taken. The raw material was collected in the flowering phase, growing in Northern Kazakstan. The VC was isolated from the plant of Tragopogon orientalis by hydrodistillation method on the Clevenger type  apparatus. The chemical composition of the VC was determined by GC/MS. The cytotoxic activity of the VC was determined by the survival rate of sea shrimp Artemia salina. VC of T. orientalis in all studied concentrations (1–10 mg ml-1) exhibit acute lethal toxicity - all larvae die. Antiradical activity was determined by the method of colorimetry of free radicals (DPPH). The experimental results showed that the VC of T. orientalis at all tested concentrations (0.1–1.0 mg ml-1) exhibit low antiradical activity compared with the butylhydroxyanisole standard drug.

Anticancer activity of leaves extracts of Convolvulus pluricaulis and Mimosa rubicaulis (Lam.) by Brine Shrimp Lethality Bioassay

2021 ◽  
pp. 5427-5432
Author(s):  
Ashpak. M. Tamboli ◽  
Kiran. A. Wadkar
Keyword(s):  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Biologically Active ◽  
Cytotoxic Activities ◽  
Significant Activity ◽  
Active Components ◽  
Brine Shrimp Lethality ◽  
Ethanolic Extracts ◽  
Convolvulus Pluricaulis

This study was subjected to investigate brine shrimp lethality bioassay of Convolvulus pluricaulis and Mimosa rubicaulis (Lam.) in different polarities of leaves extracts. Brine shrimp organisms were used to assess the cytotoxic activities of various polarities of leaves extracts while using brine shrimp lethality bioassay the cytotoxicity had been assessed and correlated with standard. Five concentrations (20, 50,100,200,500µg/ml) of each extract were used to find out the cytotoxicity. The Artemia salina lethality bioassay result was evaluated in terms of LC50 (lethality concentration).In each plant extracts into three replicates concentration naupli were added. After 24 h the surviving Artemia salina larvae were counted and LC50 was assessed. The cytotoxic activity of all polarities of extracts displayed activity within the value of LC50 (132.10–266.76 µg/ml) for Convolvulus pluricaulis and (213.86–279.47 µg/ml) for Mimosa rubicaulis (Lam.) Results revealed that the ethyl acetate extracts of Convolvulus pluricaulis and ethanolic extracts of Mimosa rubicaulis (Lam.) elicit significant activity compared to other extracts it indicated biologically active components are present in ethyl acetate and ethanolic extracts that could be accounted for its pharmacological effects.

scholarly journals SYNTHESIS, CHARACTERIZATION, AND EVALUATION OF TOXICITY AGAINST SALINE ARTEMIA OF 2,3-UNSATURATED GLYCOSIDES

2020 ◽  
Vol 17 (36) ◽  
pp. 251-262
Author(s):  
Edilma Elayne DA SILVA ◽  
Maria Verônica de Sales BARBOSA ◽  
Jucleiton José Rufino DE FREITAS ◽  
Jucarlos Rufino FREITAS ◽  
João Rufino DE FREITAS FILHO
Keyword(s):  
Biological Activity ◽  
Mortality Rate ◽  
1H Nmr ◽  
Organic Molecule ◽  
Structural Modification ◽  
Functional Group ◽  
Artemia Salina ◽  
The Other ◽  
Glycosidic Bond ◽  
Cytotoxic Activities

In the chemistry of carbohydrates, the 2,3-unsaturated O-glycoside is an organic molecule in which the saccharide unit is linked to another functional group through a glycosidic bond. Unsaturated carbohydrate derivatives are useful intermediates in synthetic transformations leading to a variety of compounds. The use of 2,3-unsaturated glycosides in glycosylation has grown exponentially during the last decade. On the other hand, there is little work in the literature that describes the synthesis and biological activity of 2,3-unsaturated Oglycoside containing the 1,2,4-oxadiazoles as aglycone. Therefore, the objective of the work is to synthesize, characterize, and evaluate its toxicity using Artemia salina. Initially, precursors, tri-O-acetyl-D-glical (7), and racemic 1,2,4-oxadiazoles (6a/b) were prepared. Then, the reaction between the tri-O-acetyl-D-glical (7) and the alcohols (R, S)-3-aryl-[1,2,4-oxadiazol-5-yl]-propan-2-ol (6a/b) was carried out using montmorillonite K-10 in dry dichloromethane as a catalyst, under reflux at a temperature of 45 °C, providing the compounds (8a/b) in excellent yields (96% compound 8a and 93% compound 8b). Compounds 8a/b synthesized were characterized based on their IR, 1H NMR, and 13C, and subsequently subjected to toxicity bioassays against the microcrustacean Artemia salina Leach. With cytotoxic activities, it was found that the mortality rate varied from 75-100% in all concentrations, which demonstrates that the compounds have potential for testing in other bioassays. The LD50 is observed at 77.21 (compound 8a) and 74.09 (compound 8b). These results can contribute to the deepening of knowledge about potential pharmacological and may be used in studies of structural modification studies to be possible pro-drugs.

Structural similarity of ribosomes from E. coli and A. salina

1978 ◽  
Vol 36 (2) ◽  
pp. 242-243
Author(s):  
M. Boublik ◽  
R.M. Wydro ◽  
W. Hellmann ◽  
F. Jenkins
Keyword(s):  
Ribosomal Proteins ◽  
Direct Evidence ◽  
Structural Similarity ◽  
Carbon Support ◽  
Artemia Salina ◽  
Uranyl Acetate ◽  
Biological Assays ◽  
E Coli ◽  
And Function ◽  
Fine Carbon

Ribosomes are ribonucleoprotein particles necessary for processing the genetic information of mRNA into proteins. Analogy in composition and function of ribosomes from diverse species, established by biochemical and biological assays, implies their structural similarity. Direct evidence obtained by electron microscopy seems to be of increasing relevance in understanding the structure of ribosomes and the mechanism of their role in protein synthesis.The extent of the structural homology between prokaryotic and eukaryotic ribosomes has been studied on ribosomes of Escherichia coli (E.c.) and Artemia salina (A.s.). Despite the established differences in size and in the amount and proportion of ribosomal proteins and RNAs both types of ribosomes show an overall similarity. The monosomes (stained with 0.5% aqueous uranyl acetate and deposited on a fine carbon support) appear in the electron micrographs as round particles with a diameter of approximately 225Å for the 70S E.c. (Fig. 1) and 260Å for the 80S A.s. monosome (Fig. 2).

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