Leaves of Cordia boissieri A. DC. as a potential source of bioactive secondary metabolites for protection against metabolic syndrome-induced in rats

2017 ◽  
Vol 72 (3-4) ◽  
pp. 107-118 ◽  
Author(s):  
Asmaa I. Owis ◽  
Amira M. Abo-youssef ◽  
Ahmed H. Osman
Keyword(s):  
Metabolic Syndrome ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Herbal Remedy ◽  
Hispanic Women ◽  
Potent Effect ◽  
Standard Drug ◽  
Potential Source ◽  
Southwestern Usa ◽  
Significant Protective Effect

Abstract Cordia boissieri A. DC. (Boraginaceae) is traditionally used as an herbal remedy for diabetes by Hispanic women in Southwestern USA. A recent investigation showed the significant protective effect of ethyl acetate extract against metabolic syndrome (MS). However, the corresponding active principles responsible for this effect and relations between their structure and biological actions remain unclear. Thus, ethyl acetate extract was subjected to column chromatography, which yielded seven compounds identified on the basis of spectroscopic data as rutin, hesperidin, kaempferol-3-O-β-d-glucopyranoside, rosmarinic acid, β-sitosterol-3-O-β-d-glucopyranoside, quercetin, and kaempferol. The isolated compounds (5 mg/kg/day) were tested in a fructose enriched-diet rat model using metformin as a standard drug. Blood samples were withdrawn for estimation of MS-associated biomarkers and liver samples were subjected to histopathological and immunohistochemical examination. The isolated compounds impaired most of the changes associated with MS as evidenced by improved insulin sensitivity, glucose tolerance, kidney function, lipid profiles and reduced oxidative stress and inflammation by different degrees. It is worth noting that quercetin and kaempferol showed the most potent effect. Structure-activity relationship study revealed that the presence of 2,3-double bond in ring C and ortho-hydroxylation in ring B increases the flavonoids activity while glycosylation or methylation decreased this activity.

scholarly journals ANTIHYPERLIPIDEMIC ACTIVITY OF COMMIPHORA MUKUL AGAINST ATHEROGENIC DIET-INDUCED HYPERLIPIDEMIA IN EXPERIMENTAL RATS

2018 ◽  
Vol 11 (6) ◽  
pp. 386
Author(s):  
Jaffar Shaik ◽  
Zeenath Khan
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Positive Control ◽  
Atherogenic Diet ◽  
Normal Diet ◽  
High Density Lipoproteins ◽  
Albino Rats ◽  
Standard Drug ◽  
Antihyperlipidemic Activity ◽  
Commiphora Mukul

Objective: The aim of the present study was to investigate the effect of antihyperlipidemic activity of ethyl acetate extract of Commiphora mukul (EECM) gum resin and compares its efficacy with that of atorvastatin against atherogenic diet-induced hyperlipidemia model using rat as an experimental animal.Methods: Wistar albino rats were divided into five groups. Control animals received normal diet, hyperlipidemic control; rats treated with EECM 100 and 200 mg/kg and positive control, receiving standard drug atorvastatin 2.70 mg/kg.Results: Increase the level of serum cholesterol, phospholipids, high-density lipoproteins, low-density lipoproteins, and triglycerides causing hyperlipidemia further leading to the development of atherosclerosis. On the other hand, oral administration of ethyl acetate extract of C. mukul at dose of 200 mg/kg for 15 days resulted in the prevention of above abnormalities.Conclusion: The results suggest that EECM could be beneficial in the treatment of atherosclerosis, characterized by atherogenic lipoprotein profile and abnormalities in lipid metabolism.

The Ethyl Acetate Extract of the Marine Edible Gastropod Haliotis tuberculata coccinea: a Potential Source of Bioactive Compounds

2021 ◽  
Author(s):  
Emiliana Tortorella ◽  
Rosa Giugliano ◽  
Marleen De Troch ◽  
Bruno Vlaeminck ◽  
Gercende Courtois de Viçose ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Ethyl Acetate Extract ◽  
Potential Source ◽  
Haliotis Tuberculata

scholarly journals Persea americana Leaf Ethyl Acetate Extract Phytochemical, In-vitro Antioxidant and In-vivo Potentials to Mitigate Oxidative Stress in Alloxan-induced Hyperglycaemic Rats

2019 ◽  
pp. 1-11
Author(s):  
J. A. Mashi ◽  
A. M. Sa’id ◽  
R. I. Idris ◽  
I. Aminu ◽  
A. A. Muhammad ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Diabetic Rats ◽  
Persea Americana ◽  
Albino Rats ◽  
Standard Drug

The purpose of this study was to investigate the in-vivo and in-vitro potentials of ethyl acetate extract of P. americana leaf in alloxan-induced diabetic rats. Quantitative phytochemicals analyzed includes; flavonoids, saponins, tannins, alkaloids and phenolics. Measurement of antioxidant activity using 1,1-Diphenyl-2-picrylhydrazyl, total antioxidant capacity, hydroxyl radical, hydrogen peroxide, superoxide radical and ferric reducing activity of the extract was carried out. Hyperglycemia was induced by intraperitoneal injection of alloxan monohydrate to albino rats. In-vivo anti-oxidant potentials of the extract were evaluated by measuring liver homogenate activity of superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase and malondyaldehyde in alloxan-induced diabetic rats administered with the extract.  A total of 30 Albino rats were used for this experiment and they were divided into six groups of 5 rats each. Group A; normal control, Group B; diabetic control, Groups C-E; experimental groups administered with different doses (100, 200 and 400 mg/kg body weight respectively); of the extract and Group F; glucophage (84 mg/kg body weight, standard drug) for 4 weeks. This study was conducted in the Department of Biochemistry, Bayero University, Kano, in August, 2018. Data was analyzed using one-way ANOVA with P=.05 value considered as significant. Results of the quantitative phytochemical investigation shows that the extract is rich in phenolics (184.1±0.6), flavonoids (115.8±2.1), alkaloids (41.5±1.8), with least concentration of tannis (21.2±0.8) and saponins (15.2±2.3). The extract exhibited high radical scavenging activity against synthetic free radicals (DPPH), reactive oxygen species (peroxide, superoxide and hydroxyl acid) and high ability to reduce Fe3+ to Fe2+ (FRAP). The activities of antioxidant enzymes of the treated rats were increased significantly (P=.05) while the level malondyaldehyde was significantly decreased (P=.05) in the treated groups. Ethyl acetate leaf extract of Persea americana contains phytochemical substances which improved antioxidant status and can be use as herbal therapy for the management of oxidative stress induced by diabetes mellitus and associated complications.

scholarly journals In-vitro antimicrobial activity of ethyl acetate extract of two common edible mushrooms

2016 ◽  
Vol 5 (2) ◽  
pp. 79-82
Author(s):  
Debendra Nath Roy ◽  
◽  
A. K. Azad ◽  
Farzana Sultana ◽  
A.S.M. Anisuzzaman ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Pleurotus Ostreatus ◽  
Ganoderma Lucidum ◽  
Ethyl Acetate Extract ◽  
Diffusion Method ◽  
Standard Drug ◽  
Gram Positive Bacteria ◽  
Growth Inhibitory ◽  
Growth Inhibitory Activity

Ethyl acetate extracts of the reishi (Ganoderma lucidum) and oyster (Pleurotus ostreatus) mushrooms were tested for their in vitro growth inhibitory activity against a panel of microorganisms of reference strains. Antimicrobial effects of both mushrooms were monitored in the dose of 400 micro gm /disc by disc diffusion method using five Gram-positive bacteria, five Gram-negative bacteria and three fungi as well. Present study showed that ethyl acetate extract of Pleurotus ostreatus has moderate and Ganoderma lucidum has only mild antibacterial effect in comparison with standard Kanamycin (30 gm. /disc). However, both mushrooms had no antifungal activity on the experimental fungi compared with standard drug Nystatin at the dose of 30 gm. /disc.

scholarly journals In vitro antioxidant and antityrosinase activities of Manilkara kauki

2021 ◽  
Vol 71 (1) ◽  
pp. 153-162 ◽  
Author(s):  
Sirinada Srisupap ◽  
Chanya Chaicharoenpong
Keyword(s):  
Ethyl Acetate ◽  
Crude Extract ◽  
Ethyl Acetate Extract ◽  
Total Phenolic ◽  
Tropical Plant ◽  
Potential Source ◽  
Antityrosinase Activity ◽  
Phenolic And Flavonoid ◽  
Different Parts

AbstractManilkara kauki L. Dubard is a tropical plant in the genus Manilkara of family Sapotaceae. This study investigated the total phenolic and flavonoid contents, and antioxidant and antityrosinase activities in different parts of M. kauki (fruits, leaves, seeds, stem barks and woods) and in fractions of stem barks. The total phenolic and flavonoid contents of the methanol and aqueous crude extracts of different parts of M. kauki ranged from 10.87 to 176.56 mg GAE (gallic acid equivalents) per gram of crude extract and 14.33 to 821.67 mg QE (quercetin equivalents) per gram of crude extract, resp. Leaves and stem barks exhibited higher total phenolic and flavonoid contents and antioxidant and anti-tyrosinase activities than fruits, seeds and woods. Stem barks were sequentially extracted with n-hexane, ethyl acetate, methanol and water and then the fractionated extracts were subjected to antioxidant and antityrosinase activities testing. The ethyl acetate and methanol extracts of stem barks exhibited higher total phenolic and flavonoid contents and antioxidant and antityrosinase activities than the n-hexane and aqueous extracts. Moreover, ethyl acetate extract of M. kauki stem exhibited the highest antityrosinase activity. It may be a potential source of tyrosinase inhibitors for pharmaceutical and cosmetic applications.

scholarly journals EVALUATION OF THE ANTIMALARIAL POTENTIAL OF STREPTOMYCES SP.

2018 ◽  
Vol 11 (2) ◽  
pp. 354
Author(s):  
Sweetline C ◽  
Usha R
Keyword(s):  
Ethyl Acetate ◽  
Tamil Nadu ◽  
Ethyl Acetate Extract ◽  
Antimalarial Activity ◽  
Antiplasmodial Activity ◽  
Control Group ◽  
Standard Drug ◽  
Streptomyces Sp ◽  
Mangrove Soil ◽  
Dose Dependent

Objective: Isolate, screen and identify Streptomyces sp. from mangrove soil from pichavaram, Tamil Nadu, India, and study the molecular identification of selected Streptomyces sp. and check the antimalarial activity for the purified compound.Methods: The16SrRNA secondary structure and the restriction sites of KMA08 were predicted using Genbank online software, respectively. Antiplasmodial activity of the 80% Ethyl acetate extract of Streptomyces sp. against chloroquine-sensitive Plasmodium berghei in mice using the 4 days suppression test was conducted. A total of 30 mice assigned to 5 groups of 6 animals each were infected with chloroquine-sensitive (P. berghei) intraperitoneally. The Ethyl acetate extract (10, 20, and 30 ml/kg), standard drug (chloroquine, 10 mg/kg) and distilled water were administered orally daily for the treatment period. Percent Parasitemia was determined on the 5th day from Giemsa stained smears obtained from tail vein and percent parasitemia suppression was calculated. Daily measurement of rectal temperature was also taken while body weight and packed cell volume were recorded on day 0 and 5.Results: Results showed the extract produced a dose-dependent reduction in parasite density compared to the control group. Percept parasitemia calculation revealed 21.3, 65.3, and 80.5% inhibition at 10, 20, and 30 ml/kg of the extract, respectively.Conclusion: The study revealed the present work indicated that Streptomyces sp. has as promising antiplasmodial activity against chloroquine sensitive P. berghei in a dose-dependent. As part of the drug discovery process, these promising finding may contribute to the medicinal and pharmaceutical field for malarial treatment.

scholarly journals An Evaluation of The Antifungal and Antioxidant Activity of Pinus merkusii Bark Ethyl Acetate Extract

2021 ◽  
Vol 15 (1) ◽  
pp. 102-110
Author(s):  
Masendra Masendra ◽  
Brandon Aristo Verick Purba Purba ◽  
Ganis Lukmandaru
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Growth Inhibition ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Antioxidant Compounds ◽  
Potential Source ◽  
Antioxidant Agents ◽  
Pinus Merkusii ◽  
Ethyl Acetate Extracts

Pinus merkusii is the only species in the Pinaceae family to grow in Southeast Asia, including Indonesia, and serves as a source of wood and oleoresins. During the utilization of this pine, the bark is discarded as residue, however, this residue is possibly a potential source of antifungal and antioxidant agents, due to the polyphenol contents. This study, therefore, aims to investigate the antifungal and antioxidant compounds present in the bark of P. merkusii. The ethyl acetate extracts of Pinus merkusii were fractionated to obtain five fractions (Fr. A- Fr. E) and the bioactivity of each fraction was observed. Subsequently, the growth inhibition against Phanerochaete chrysosporium, was evaluated for antifungal activity, while DPPH (1,1-diphenyl-2-picrylhydrazyl) method was used to ascertain antioxidant activity. According to the GC-MS analysis, the ethyl acetate fractions contain triterpenoids and steroids as the dominant compounds as well as minor amounts of mono-sesquiterpenoids and hydrocarbons. The highest growth inhibition exhibited by Fr. C- Fr. E (>60%) indicates triterpenoids and steroids act as a fungal inhibitors. Meanwhile, the five fractions exhibited low antioxidant activity because the steroids and triterpenoids present are possibly ineffective DPPH inhibitors. Also, the antifungal and antioxidant activity R2 values were discovered to correlate 0.3, indicating a low positive correlation.

scholarly journals The Chemical Composition of Gracilaria verrucosa Extract and its Utilization on Survival and Growth Litopenaeus vannamei

Omni-Akuatika ◽  
2018 ◽  
Vol 14 (3) ◽  
Author(s):  
Yudiana Jasmanindar ◽  
Sukenda Sukenda ◽  
Alimuddin Alimuddin ◽  
Muhammad Zairin Junior ◽  
Nur Bambang Priyo Utomo
Keyword(s):  
Chemical Composition ◽  
Ethyl Acetate ◽  
Litopenaeus Vannamei ◽  
Crude Protein ◽  
Ethyl Acetate Extract ◽  
Gracilaria Verrucosa ◽  
Test Diet ◽  
Potential Source ◽  
Diet Control ◽  
Survival And Growth

The Gracilaria genus is a potential source of natural and environmentally-friendly alternatives in improving the survival and growth of shrimp. This study aims to identification immunostimulant molecules extract G. verrucosa and evaluate the utilization of G verrucosa extract as an immunostimulant in improving survival and growth of L. vannamei. Seaweed extraction used ethyl acetate then formulated in the diets. The immunostimulant molecule in the G. verrucosa was analysis. The shrimp were fed a test diet containing extract G. verrucosa at a dose of 2 g kg-1 or extract G. verrucosa-free control diets for 42 days. Shrimps were fed diets containing extract with a specific duration. The observation on the survival and growth of L. vannamei was performed after maintenance at the Laboratory for six weeks. Following, diets containing extract was tested in the field (pond shrimp farm) at the same dose of extract for 58 days. Shrimp was feed diets containing extract once a week, once in the early culture, and diet control, then the survival and growth shrimp were analysis. Concentrations of sulfates and carbohydrates in G. verrucosa ethyl acetate-extract were 24.21% and 13.41%, and crude protein 3.64%. GC-MS pyrolysis results show that G. verrucosa polysaccharide is similar to immunostimulant molecules. The survival shrimp gave diets containing G. verrucosa extract formulation was higher than that of shrimps fed controls diet. The Shrimp fed diets extracts have higher growth than shrimp given control dietsKeywords: Gracilaria, extract, polysaccharides, immunostimulant

Antinociceptive profile of ethyl acetate extract of Hyptis fruticosa Salzm. ex Benth. (Labiatae)

Planta Medica ◽  
2006 ◽  
Vol 72 (11) ◽  
Author(s):  
RM Marçal ◽  
DM Ptak ◽  
RR Krempser ◽  
MR Krempser ◽  
SV Floresta ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract

Phytochemical screening for identification of bioactive compounds of Lantana camara Linn. (Verbenaceae) responsible for larvicidal action against Aedes triseriatus Say, 1823 (Diptera : Culicidae)

ENTOMON ◽  
2019 ◽  
Vol 44 (2) ◽  
pp. 117-126
Author(s):  
Rhitayu Chakraborti ◽  
Probir Kumar Bandyopadhyay
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Active Ingredient ◽  
Crude Extract ◽  
Ethyl Acetate Extract ◽  
Lantana Camara ◽  
Mosquito Larvae ◽  
Aedes Triseriatus ◽  
Phytochemical Screening ◽  
Lc50 Value

Study to assess the larvicidal property of Lantana camara leaves against Aedes triseriatus larvae found that the ethyl acetate extract had profound larvicidal action with the crude extract having a LC50 value of 409.831ppm. GC-MS analysis of the ethyl acetate extract confirmed the presence of twenty-one compounds out of which beta-caryophyllene covered the highest percentage of the chromatogram area. Further tests with beta-caryophyllene against the mosquito larvae proved it to be the active ingredient of L. Camara with a LC50 value of 104.243ppm.

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