Possible circadian periodicity of foetal prolactin secretion late in gestation

1981 ◽  
Vol 98 (1) ◽  
pp. 106-111 ◽  
Author(s):  
Tsutomu Yaginuma

Abstract. Prolactin levels were determined in samples of umbilical venous blood at vaginal delivery after 38–42 weeks of uncomplicated gestation. The mean level of prolactin for all samples was 328.4 ± 7.4 (se) ng/ml. The levels did not vary significantly during these weeks of gestation, nor correlate with the duration of labour. The means of levels combined every 2 h varied significantly throughout the 24 h period with a maximum value of 460.3 ± 85.1 ng/ml at 2–4 a.m. and a minimum value of 241.4 ± 27.2 ng/ml at 6–8 a.m. and remained relatively constant between 2–4 and 10–12 p.m. These results suggest that a circadian periodicity may exist in the secretion of prolactin by the human foetus, and that this is not affected by labour and delivery.

Diagnostics ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 94 ◽  
Author(s):  
Mariella Dipalo ◽  
Cecilia Gnocchi ◽  
Paola Avanzini ◽  
Roberta Musa ◽  
Martina Di Pietro ◽  
...  

Our laboratory performs procalcitonin (PCT) assays on a Brahms KRYPTOR analyzer with the Brahms PCT sensitive Kryptor kit. In this study, we wanted to compare the assays obtained in this way with the ones performed on the LIAISON® XL. From January to May 2017, 171 samples were analyzed, of which 65 from female patients (age: 22–98 years) and 106 from male patients (age: 16–97 years). The PCT determination was performed using the LIAISON® XL and KRYPTOR analyzers, by chemiluminescence (Chemiluminescence immunoassay—CLIA) (LIAISON® BRAHMS PCT® II GEN) and immunofluorescence (Brahms PCT sensitive Kryptor) assay, respectively. For the LIAISON® BRAHMS PCT® II GEN, 52% of the results were placed between 0.0 and 0.5 ng/mL, 18% between 0.5 and 2.0 ng/mL, and 30% between 2.0 and 100 ng/mL; the mean was 4.09 ng/mL, the median 0.456 ng/mL, the maximum value 97.2 ng/mL, and the minimum value 0.02 ng/mL. For the Brahms PCT sensitive Kryptor, 55% of the results were positioned between 0.0 and 0.5 ng/mL, 21% between 0.5 and 2.0 ng/mL, and 24% between 2.0 and 100 ng/mL; the mean was 3.72 ng/mL, the median 0.39 ng/mL, the maximum value 103 ng/mL, and the minimum value 0.01 ng/mL. The mean of the results obtained with the two methods showed no significant differences (3.717 for Kryptor and 4.094 for LIAISON®). PCT assay with Brahms reagents, both on the Kryptor and LIAISON®XL platforms, offers excellent performance in terms of sensitivity and specificity.


Author(s):  
Mulia Mayangsari

The research design used was Experimental with Kunsekutive Sampling technique. The population in this study were patients who had migraine in Noreh village. The working area of Puskesmas Sreseh was 18 respondents and the result was analyzed using Shapiro Wilk and Wilcoxon test. The results of the study in migraine patients can be seen that the mean pretest scale of migraine pain scale is 4.67 whereas, for the mean posttest of migraine pain scale is 2.22. In the pretest of migraine pain scale the minimum value found is 4 whereas, the maximum value is 6. While in posttest skalanyeri migraine the minimum value found is 1 whereas, the maximum value is 3. In the Shapiro Wilk analysis showed that there were differences in the scale of migraine pain before and after wet bruise therapy. In the Wilcoxon analysis comparing migraine patients, a p-value of 0,000 indicated that there was an effect of wet-brushing therapy on changes in the pain scale. To health workers and educational institutions are expected to use wet brewing therapy as a complementary alternative and as a non-pharmacological treatment that can reduce the scale of pain.


Author(s):  
Ya. I. Sheiko ◽  
D. A. Zhmoidyak

The article presents the results of comparison of the biochemical composition of the muscles of two-year carp of two-way crosses and the breeds and lines of different-origin carp and Amur sazan. The combinations characterized by an increased level of the dry basis content, fat and protein are established in comparison with a mean-population value, a mean level of the Belarusian collection lines, import breeds and Amur sazan bred under the same conditions. The mean mass of two-year carp selected for investigation was 531.3 g within the range from 370.2 to 645.0 g mainly with a low and mean degree ofvariation. The mean level of the dry basis content of all examined crosses was 25.85 %, the maximum value of this parameter of German × three prim was 27.92 %, and the minimum value of Germany ×Lahvin scaleless was 24.08 %. The low values of the variation coefficient (1.0–9.8 %) point to a slight variation of this parameter of different mixed bred crosses.


2003 ◽  
Vol 58 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Luiz Airton Saavedra de Paiva ◽  
Marco Segre

OBJECTIVE: The purpose of this study was to evaluate the significance for sex determination of the measurement of the area formed by the xerographic projection of 3 craniometric points related to the mastoid process: the porion, asterion, and mastoidale points. METHOD: Sixty skulls, 30 male and 30 female, were analyzed. A xerographic copy of each side of the skull was obtained. On each xerographic copy, the craniometric points were marked to demarcate a triangle. The area (mm²) of the demarcated triangle for each side of the skull (right (D) and left (E) sides) was determined, and the total value of these measures (T) was calculated. RESULTS: Concerning the right area of the male and female skulls, 60% of the values overlapped; for the left area, 51.67% overlapped, and for the total area, 36.67% overlapped. The analysis of the differences between the sexes in the areas studied was significant for the 3 areas. Regarding the total area, which is the preferred measurement because of the asymmetry between the sides of the skull, the value of the mean was 1505.32 mm² for male skulls, which was greater than the maximum value obtained in the female skulls. The value of the mean for female skulls was 1221.24 mm², less than the minimum value obtained for the male skulls. CONCLUSIONS: This study demonstrates a significant result in the 3 studied areas, (D), (E), and (T). The total area values show less overlapping of values between the sexes, and therefore can be used for sexing human skulls. For the population studied, values of the total area that were greater than or equal to 1447.40 mm² belonged to male crania (95% confidence). Values for this area that were less than or equal to 1260.36 mm² belonged to female crania (95% confidence).


Author(s):  
Shaymaa H. Jasim, Wisam A. Radhi, Riyadh M. Ramadhan, Raed M

The extinction of X-rays (radiation attenuation) was studied using the low-density samples of polyethylene polymer to which the rockwool fibers powder is added as filled filler. This latter was blended with (weight percent) and with a micro-filler (filler particle) the sizes equal to or less than<212 μm. Furthermore, the free path average and linear attenuation coefficient were calculated.  Experimental results showed that the rockwool fibers powder act to reduce the spaces between polymer chains particularly when the weight percent is more than (10%), which implies the capability of the polymer/filler to make, the X-rays applied to the samples; disappear at these rates used in this study. The experimental work was conducted by applying a radiation beam having an energy of 30 kV based on the use of the X-ray unit with two tubes which are; X-ray generating tube and G-M detector with an energy of VG.M =600. The magnitudes of the mean free path are inversely proportional to the weight percent of the compound material whereas the proportionality of these percentages which are particularly the high ones which occur at experimental values of the linear attenuation coefficient. The value of the mean free path of 1.28 cm is the maximum value obtained at a weight percent of 1 %, whereas the minimum value of the mean path was 0.877 cm at a weight percent of 10 %. In addition, the maximum value of the attenuation coefficient obtained is 4.754 cm-1 at a weight percent of 10% and its minimum value at a weight percent of 1% was 0.7 cm-1. The maximum value of transmittance and the minimum value of absorbance were obtained at a weight percent of 10%, are (31.8) and (68.2) respectively. Through the practical results that we obtained that are better suited to high percentages more additive proportions can be used than the percentages used in this research to shielding X-rays more.


Author(s):  
Mahmoud Showky Mohammed Ahmed, Rasha Nabil Sami, Noha Adel E Mahmoud Showky Mohammed Ahmed, Rasha Nabil Sami, Noha Adel E

The aim of the study was to assess fracture resistance under cyclic loading of Rosetta®SM CAD vs. IPS e.max CAD (control) vonlays restoring maxillary premolars. According to the sample size calculation, a total of 20 samples will be selected and then divided into two equal groups; 10 sample each based on the material type: Group (A): Ten vonlays (n=10) manufactured from IPS e. max CAD blocks (control). Group ( B): Ten vonlays (n=10) manufactured from Rosetta®SM CAD blocks (intervention). A natural tooth presenting an upper first premolar was prepared in accordance with the ceramic onlay restorations preparation guidelines with an occlusal box with half of the bucco-lingual distance and a 2 mm depth from the cusp tip to pulpal floor, gingival seat with 1 mm depth from pulpal floor, occlusal reduction of 2 mm of the functional cusp with extending the preparation at the palatal surface by 2 mm in the cervical direction, occlusal reduction of 1.5 mm of non-functional cusp and 12º divergence angle. The preparation was then extended to the labial surface, with a 0.5 mm chamfer finish line. All line angles were finally rounded, and all margins were finished. For duplication of the natural tooth; the epoxy dies were constructed. Base and catalyst of the epoxy resin material were mixed as instructed by the manufacturer at a rate of 200r/min and then was poured into the silicon mould under vibration to eliminate any air voids, then was allowed to set completely following the manufacturer instructions for 24 hours, this procedure was repeated 20 times for creating 20 epoxy resin dies which replicating the prepared natural tooth. The die was then scanned by optical scanning using Medit i500 oral scanner. Milling of Vonlays was achieved using Sirona MCX5 milling machine using IPS e. max CAD blocks and Rosetta SM CAD blocks: 10 IPS e.max CAD blocks with block size C14 and 10 Rosetta®SM CAD blocks with block size C14 were used. Vonlays were then seated on their corresponding dies and cemented with dual cured self-adhesive resin cement (Variolink, Ivoclar Vivadent). Cementation procedures were performed by keeping each sample under a constant pressure of 5Kg using a custom designed cementation device for standardization the applied load during cementation process. All samples were mechanically aged (cyclic loading) to simulate 6 months of clinical use. Following that, all samples were subjected to fracture resistance testing under a compressive load to the center of their long axis using computer controlled universal testing machine with a load cell of 5 Kilo newton (K N) with a computer software used for data recording (Instron® Bluehill Lite Software). The mean values ± SDs values for e.max group were (502.39 ±102.89 N) with minimum value (338.16 N) and maximum value (721.29 N). The mean values ± SDs values. Rosetta group were (468.76 ± 67.54 N) with minimum value (367.11 N) and maximum value (648.48 N). Failure modes were determined under stereomicroscope and classified into repairable and catastrophic in both groups and showed that 60% of e.max group exhibited repairable failure while 40% of specimens showed catastrophic failure while in Rosetta group, 90% of the specimens exhibited repairable failure. Only 10% displayed catastrophic failure. Then a fractured part within each group was examined under scanning electron microscope (SEM) to reveal and compare their microstructure.


2020 ◽  
Vol 16 (2) ◽  
pp. 125-133
Author(s):  
Zahra Rezaieyazdi ◽  
Sima Sedighi ◽  
Masoumeh Salari ◽  
Mohammadreza H. Fard ◽  
Mahmoud R. Azarpazhooh ◽  
...  

Background: The relationship between SLE and traditional risk factors for cardiovascular events was evaluated. Methods: The data regarding sixty patients with SLE and 30 healthy controls (age and sex matched) were gathered using SLEDAI forms. Venous blood (10mL) from all the participants was examined for hs-CRP, homocysteine, VCAM1, CBC, anti-DNA antibody, C3, C4, low-density lipoprotein (LDL), cholesterol, FBS and triglyceride. : The IMT of carotid arteries was determined bilaterally by ultrasound. Other measurements included insulin levels via Elisa (Linco/Millipore Corp) and the HOMA-IR index for insulin resistance. Results: The mean age (in years) in the test and control groups was 28.8±10.3 (18-52) and 33.8±9.13 (18-48), respectively. Results: The mean age (in years) in the test and control groups was 28.8±10.3 (18-52) and 33.8±9.13 (18-48), respectively. : The average IMT in the test group was directly related to serum levels of VCAM1 (p<0.001), homocysteine (p<0.001), cholesterol (p<0.009), LDL (p<0.001), TG (p<0.001), and FPG (p=0.004). The association between other risk factors, insulin resistance, carotid IMT and SLEDAI, was nonexistent. Mean insulin and insulin resistance levels in all the participants were 0.43±2.06 µU/mL and 0.09±0.44, respectively. There was no significant difference between the test and control groups regarding serum insulin and insulin resistance levels (p=0.42 and p=0.9, respectively). None of the risk factors, such as hsCRP, VCAM1, or homocysteine, were shown to be related to insulin resistance (p=0.6, p=0.6, p=0.09, respectively). Conclusion:: Our findings did not show an increase in the prevalence of atherosclerosis in patients with SLE. There was no association between IMT and insulin resistance. However, the former was associated with FPG, total cholesterol, LDL, TG, homocystein and VCAM1.


2020 ◽  
Vol 7 (04) ◽  
Author(s):  
SATYA NARAYAN SINGH ◽  
RAJESH G BURBADE ◽  
HITESH SANCHAVAT ◽  
P S PANDIT

The cereals of today are more nutritious and healthful than ever before. Cereals processing is one of the oldest and the most essential part of all food technologies. Pasta products and noodles have been staple foods since ancient times in many countries all over the world. In this study pasta formulation was substituted with blending sapota powder in different proportions (4 levels i.e. 0%, 10%, 20%, 30%) into semolina and maida flour separately. Pasta products were prepared using eight different formulations and adding water (approximately 31% of total weight) in DOLLY pasta extruder machine. All the samples were evaluated for physical properties: specific length (mm/g), bulk density (kg/m3), specific density (kg/m3) and porosity (%); functional properties: water absorption index (%), water solubility index (%) and oil absorption capacity (ml/g) and nutritional compositions: moisture (%), crude protein (%), fat (%) and carbohydrate (%). Highest specific length 36.20 mm/g was observed for T5 treatment, low bulk density 368.10 kg/m3 was observed for T5 and highest porosity 9.24% was found for T1 treatment. The maximum WAI, WSI values 325.83%, 17.33% respectively was observed for T1 treatment and minimum value of oil absorption capacity 1.06 ml/g for T8 treatment. The moisture content of dried pasta products was found in the range of 6 to 7%. The maximum value of crude protein 13.07% was found for T5 and minimum value 8.81% for T4 treatments. The fat contents were varied from 1.02% to 1.28 %. The maximum value of carbohydrate was 76.20% for T1 and minimum value 65.41% for T8.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xiaoling Zhang ◽  
Jingjing Zhang ◽  
Jiamei Li ◽  
Ya Gao ◽  
Ruohan Li ◽  
...  

AbstractEvidence indicates that glucose variation (GV) plays an important role in mortality of critically ill patients. We aimed to investigate the relationship between the coefficient of variation of 24-h venous blood glucose (24-hVBGCV) and mortality among patients with acute respiratory failure. The records of 1625 patients in the Multiparameter Intelligent Monitoring in Intensive Care II (MIMIC II) database were extracted. The 24-hVBGCV was calculated as the ratio of the standard deviation (SD) to the mean venous blood glucose level, expressed as a percentage. The outcomes included ICU mortality and in-hospital mortality. Participants were divided into three subgroups based on tertiles of 24-hVBGCV. Multivariable logistic regression models were used to evaluate the relationship between 24-hVBGCV and mortality. Sensitivity analyses were also performed in groups of patients with and without diabetes mellitus. Taking the lowest tertile as a reference, after adjustment for all the covariates, the highest tertile was significantly associated with ICU mortality [odds ratio (OR), 1.353; 95% confidence interval (CI), 1.018–1.797] and in-hospital mortality (OR, 1.319; 95% CI, 1.003–1.735), especially in the population without diabetes. The 24-hVBGCV may be associated with ICU and in-hospital mortality in patients with acute respiratory failure in the ICU, especially in those without diabetes.


Author(s):  
Gomathi Ramaswamy ◽  
Kashish Vohra ◽  
Kapil Yadav ◽  
Ravneet Kaur ◽  
Tripti Rai ◽  
...  

Abstract Introduction Globally around 47.4% of children and in India, 58% of children aged 6–59 months are anemic. Diagnosis of anemia in children using accurate technologies and providing adequate treatment is essential to reduce the burden of anemia. Point-of-care testing (POCT) devices is a potential option for estimation of hemoglobin in peripheral and field settings were the hematology analyzer and laboratory services are not available. Objectives To access the validity of the POCTs (invasive and non-invasive devices) for estimation of hemoglobin among children aged 6–59 months compared with hematology analyzer. Methods The study participants were enrolled from the pediatric outpatient department in Haryana, India, from November 2019 to January 2020. Hemoglobin levels of the study participants were estimated in Sahli’s hemoglobinometer and invasive digital hemoglobinometers (DHs) using capillary blood samples. Hemoglobin levels in non-invasive DH were assessed from the finger/toe of the children. Hemoglobin levels measured in POCTs were compared against the venous blood hemoglobin estimated in the hematology analyzer. Results A total of 120 children were enrolled. The mean (SD) of hemoglobin (g/dl) estimated in auto-analyzer was 9.4 (1.8), Sahli’s hemoglobinometer was 9.2 (1.9), invasive DH was 9.7 (1.9), and non-invasive DH was 11.9 (1.5). Sahli’s hemoglobinometer (95.5%) and invasive DH (92.2%) had high sensitivity for the diagnosis of anemia compared with non-invasive DH (24.4%). In contrast, non-invasive DH had higher specificity (96.7%) compared with invasive DH (83.3%) and Sahli’s hemoglobinometer (70%). Invasive DH took the least time (2–3 min) for estimation of hemoglobin per participant, followed by Sahli’s (4–5 min) and non-invasive DH (5–7 min). Conclusion All three POCT devices used in this study are reasonable and feasible for estimating hemoglobin in under-5 children. Invasive DHs are potential POCT devices for diagnosis of anemia among under-5 children, while Sahli’s can be considered as a possible option, where trained and skilled technicians are available. Further research and development are required in non-invasive DH to improve accuracy. Lay summary In India, anemia is a serious public health problem, where 58% of the children aged 6–59 months are anemic. Point-of-care testing (POCT) using digital hemoglobinometers (DHs) has been recommended as one of the key interventions by the Anemia Mukt Bharat program since 2018 in India. These POCT devices are easy to use, less invasive, can be carried to field, require minimal training and results are available immediately. Therefore this study assessed the validity of POCT devices—invasive DH, non-invasive DH and Sahli’s hemoglobinometer among 6–59 months children in facility setting compared with the gold standard hematology analyzer. A total of 120 children under 6–59 months of age were enrolled from the pediatric outpatient department in Haryana, India, from November 2019 to January 2020. The (mean hemoglobin in g/dl) invasive (9.7) and non-invasive DH (11.9) overestimated hemoglobin value, while Sahli’s (9.2) underestimated hemoglobin compared with hematology analyzer (9.4). Invasive DH (92.2%) and Sahli’s hemoglobinometer (95.5%) reported high ability to correctly identify those with anemia compared with non-invasive DH (24.4%). In contrast, non-invasive DH (96.73%) had higher ability to correctly identify those without the anemia compared with invasive DH (83.3%) and Sahli’s (70%).


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