scholarly journals Impact of gibberelic acid and tebuconazole on formation of the leaf system and functioning of donor – acceptor plant system of solanaceae vegetable crops

2017 ◽  
Vol 8 (2) ◽  
pp. 162-168 ◽  
Author(s):  
V. H. Kuryata ◽  
V. V. Rogach ◽  
O. I. Buina ◽  
O. V. Kushnir ◽  
O. V. Buinyi
Keyword(s):  
Mass Fraction ◽  
Leaf Surface ◽  
Photosynthetic Apparatus ◽  
Crop Productivity ◽  
Vegetable Crops ◽  
Tomato Plants ◽  
Vegetative Organs ◽  
Net Productivity ◽  
Donor Acceptor ◽  
Gibberelic Acid

We studied the comparable effect of gibberelic acid and tebuconazole on morphogenesis, mesostructure formation and redistribution of flows in sweet peppers and tomatoes. It has been found that the use of gibberelic acid and tebuconazole retardant during budding leads to increased plant productivity due to optimization of the structure and operation of the plants’ leaf apparatus. It was established that both gibberelic and antigibberelic tebuconazole drug stimulated the formation and functioning of the photosynthetic apparatus of peppers and tomatoes, but the mechanisms of this regulation were different. Increased photosynthetic activity of plants under the influence of gibberellin was determined primarily by the formation of more leaves and total leaf surface. When using tebuconazole retardant there was a significant restructuring of the organization of leaf mezostructure: the leaves were thickened by chlorenchyma proliferation, there was an increase in the volume of columnar parenchyma cells and linear dimensions of spongy parenchyma leaf cells. The surface density of leaves significantly increased, the chlorophyll content and nitrogen content (especially protein) also increased, compared with control variants and variants using gibberelin. Such a profound restructuring of the photosynthetic apparatus in plants under the actions of tebuconazole led to a significant increase in donor leaves function of peppers and tomatoes, which is an indicator of the growth of net productivity of photosynthesis – the highest among all the variants of the experiment. The results also show that increasing the chlorophyll phytocenotic index was more significant than the increase of leaf index: the tomatoes under the action of tebuconazole had a lower leaf index than in control options, but due to a higher chlorophyll index the crop productivity increased.Since during the fruiting period the costs of assimilates to the growth of vegetative organs are greatly reduced, optimization of photosynthetic apparatus in pepper and tomato plants led to the laying of more fruit per plant and increasing crop yield. The analysis of the mass ratio of the researched vegetative and fruit plants shows that the mass fraction of fruit (an acceptor sphere of plants during fruiting) under the action of both drugs increased. Thus in both variants of the experiment both the mass fraction and donor assimilates of leaves were higher. Apart from the main source of assimilates – the processes of photosynthesis, which intensified due to the formation of a larger area of leaf surface (variant with gibberelin) or optimization of mesostructure (variant with tebuconazole) it is probable that the formation and growth of the embryo occurred in part due to reutilization of carbohydrates from the vegetative plant organs in carpogenesis processes. 

scholarly journals Dynamic of accumulation and redistribution of various carbohydrate forms and nitrogen in organs of tomatoes under treatment with retardants

2018 ◽  
Vol 9 (2) ◽  
pp. 293-299 ◽  
Author(s):  
V. V. Rogach ◽  
O. O. Kravets ◽  
O. I. Buina ◽  
V. G. Kuryata
Keyword(s):  
Nitrogen Content ◽  
Total Nitrogen ◽  
Reducing Sugars ◽  
Sugar Content ◽  
Total Nitrogen Content ◽  
Average Weight ◽  
Growth Processes ◽  
Tomato Plants ◽  
Vegetative Organs ◽  
Donor Acceptor

The regulation of plant growth and development under application of antigibberellin compounds is accompanied by changes in the donor-acceptor system that in turn affects the accumulation and redistribution of carbohydrates and nitrogen-containing compounds. We studied the influence of tebuconazole 0.025% (EW-250) and 0.05% and 0.07% esphon (2-CEPA) on growth processes, leaf apparatus and content of various carbohydrate forms and total nitrogen content in vegetative organs of tomato hybrids Bobkat and Solerosso. A field-based micro-trial setup was established from 2013 to 2017. The plants were treated via foliar spraying with aqueous solution of retardants at the time of initiation of budding. Phytometric measurements were determined every 10 days, the content of various carbohydrate forms and total nitrogen in organs of plants was determined by using biochemical methods in a fixed dry material (the content of sugars and starch – by iodometric method, and the total nitrogen content – by Kjeldahl). It was found that both retardants reduced the linear growth of tomato plants. The results indicate that 2-CEPA treatment significantly decreased the plant height of both hybrids. Application of drugs increased the number of leaves per plant. The leaf dry matter weight increased, the most significant effect was created by EW-250. The leaf area of the retardant-treated tomatoes hybrid Solerosso increased and decreased in the hybrid Bobcat. Application of antigibberellin compounds intensified the outflow of sugars and remobilization of starch in roots and stems of both tomato hybrids. The content of starch, reducing and non-reducing sugars in leaves of hybrid Solerosso was higher after EW-250 treatment. 2-CEPA increased the content of reducing form of sugars, and EW-250 increased non-reducing form in the hybrid Bobkat. EW-250 intensified the accumulation of carbohydrates in fruits due to reducing sugars in both tomato hybrids, whereas 2-CEPA reduced their content compared with control. Growth inhibitors increased the starch content in fruits at the beginning of vegetation and intensified its reutilization at the end. This process was most intensive was in variant of EW-250-treated plants regardless of hybrids. We proved that there was a clear tendency to decrease the ratio of sugar content to starch in almost all organs of both tomato hybrids during the growing season. The most significant was the reduction of this ratio after EW-250 treatment. At the fruitification phase, the proportion of starch in retardant-treated plants increased compared to the control. The increased accumulation of carbohydrates in tomato fruits treated with EW-250 caused an increase in the average weight of the fruit. We have found that the total nitrogen content in vegetative organs decreased in the control and in the experimental variant of both tomato hybrids during vegetation. EW-250 accelerated the outflow of total nitrogen from roots and stems and increased its content in leaves. This drug retarded the accumulation of this element in fruit. Such changes in the dynamics of total nitrogen under the influence of tebuconazole resulted in enhanced formation of fruit on tomato plants. We concluded that the antigibberellin compounds EW-250 and 2-CEPA due the action on growth processes and leaf apparatus of tomato plants, changed the character of donor-acceptor relation through an increase in outflow of plastic substances from vegetative organs to fruits, which optimized the crop production.

scholarly journals Features of morphogenesis, accumulation and redistribution of assimilate and nitrogen containing compounds in tomatoes under retardants treatment

2018 ◽  
Vol 8 (1) ◽  
pp. 356-362 ◽  
Author(s):  
V. G. Kuryata ◽  
O. O. Kravets
Keyword(s):  
Morphological Changes ◽  
Crop Productivity ◽  
Tomato Crop ◽  
Tomato Plants ◽  
Vegetative Organs ◽  
Palisade And Spongy Parenchyma ◽  
Source Sink ◽  
Sphere Formation ◽  
Nitrogen Containing Compounds

<p>We studied the influence of ethylene product Esphon® and triazole derivative Folicur® on the morphogenesis and productivity of tomatoes. The obtained results explained the significant role of morphological and mesostructural components of “source-sink” relation system of tomatoes in the formation of crop productivity. We established that the Folicur application resulted in more significant anatomical and morphological changes in the formation of leaf apparatus in comparison with the ethylene producer: the measurement of number, weight, area of leaf surface, and leaf index were higher in this variant of experiment. We also noted the appropriate changes in the mesostructure measurement of leaves: thickness of leave – main photosynthetic tissue of chlorenchyma, assimilatory cells size of palisade and spongy parenchyma. Analysis of depositing possibilities of plants vegetative organs at the fruitification stage indicates the importance of temporary postponement of nonstructural carbohydrates and nitrogen containing compounds in them followed by reutilization for carpogenesis needs. Application of Folicur resulted on the formation of a more powerful donor sphere and in the early fruit growth and formation stages are postponed more carbohydrates in vegetative organs of tomato plants compared to control. The content of sugars and starch in the roots, stems and leaves of plants under Folicur treatment was highest in all organs of the plant throughout the fruitification phase compared to control and variant with Esphon application. Our results also testify to the possibilities of nitrogen compounds remobilize from vegetative organs to carpogenesis needs. After Folicur treatment, the most intense decrease in the content of nitrogen containing compounds was observed during the transition from the fruit formation stage to green fruits stage in roots and stem of plant during the period of the most intense tomatoes growth. We concluded that a significant increase the yield of tomato crop was caused by the more powerful donor sphere formation and reutilization of assimilates and nitrogen containing compounds after Folicur application.</p>

scholarly journals АНАТОМІЧНА БУДОВА РОСЛИН GLYCINE MAX MOENCH. ЗА ДІЇ ШТАМУ BRADYRHIZOBIUM JAPONICUM ТА РЕТАРДАНТУ

2020 ◽  
Vol 80 (3-4) ◽  
pp. 98-104
Author(s):  
L. A. Golunova
Keyword(s):  
Growth Regulators ◽  
Bradyrhizobium Japonicum ◽  
Leaf Surface ◽  
Mutual Effect ◽  
Crop Productivity ◽  
Reproductive Organ ◽  
Nodule Bacteria ◽  
Vegetative Organs ◽  
Chlorophyll Accumulation ◽  
Leaf Surface Area

The effect of nodule bacteria strain Bradyrhizobium japonicum and the integrated use of the strain and retardant of the triazole class - tebuconazole on linear growth, anatomical structure of Glycine plants and their influence on crop productivity were studied in the field experiment. It was found that both the aplication of Bradyrhizobium japonicum M 8 strain solely and the complex use of the preparations (treatment of the plants with retardant at the beginning of reproductive organ formation, against presowing inoculation of soybean seeds) affected experimental plants’ height, histological parameters of the vegetative organs in comparison with those of untreated control. The use of growth regulators caused changes in the leaf surface area, mesostructural organization of the leaves and accumulation of photosynthetic pigments in them. There were also indicated observable changes in the size of the bark and mechanical tissues of the stem. The effect of the restrictive growth regulators was apparant in the following: the strain of nodule bacteria caused an increase in the linear size of the stem and leaf surface of plants against control. The mutual use of tebuconazole- retardant and strain Bradyrhizobium japonicum led to a decrease in apical dominance of plants, leaf thickness due to increased cell size of wall and spongy parenchymas and intensification of chlorophyll accumulation. Treated plants were characterized by changes in the size of their bark and structural tissues of the stem (collenchyma and sclerenchyma), which is a prerequisite for higher resistance to lodging of plants. The mutual effect of Bradyrhizobium japonicum strain and the triazole preparation proved to be most efficacious among the preparations used. Exogenous application of tebuconazole against the strain of nodule- forming bacteria contributes essentially to the strength of the stem, improves donor functions of Glycine hispida Max, which further secures growth and development of generative organs towards improving yield capasity.

scholarly journals Development and Yield Traits Indicate That the Constitutive Wound Response Phenotype of Prosystemin Overexpressing Tomato Plants Entails No Fitness Penalty

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1148
Author(s):  
Mariela Luna Martínez ◽  
Norma Martínez-Gallardo ◽  
Kena Casarrubias-Castillo ◽  
Simona M. Monti ◽  
Mariangela Coppola ◽  
...  
Keyword(s):  
Intrinsically Disordered Protein ◽  
Titratable Acidity ◽  
Peptide Hormone ◽  
Defense Responses ◽  
Crop Productivity ◽  
Photosynthetic Performance ◽  
Wound Response ◽  
Tomato Plants ◽  
Intrinsically Disordered ◽  
Environmentally Sound

Systemin is a peptide hormone that regulates the wound response in tomato plants. Consequently, the overexpression of its prosystemin (ProSys) precursor protein leads to a resource-demanding constitutive activation of tomato’s wound-response. According to the growth vs. defense resource allocation premise, ProSys overexpression should negatively affect the physiological fitness of tomato plants. The present study was performed to explore why the opposite effect was steadily observed, instead. It was based on the premise that a better understanding of this unexpected outcome could help establish improved wound and related defense responses without negatively affecting crop productivity. To this effect, an experimental strategy was deployed to measure various physiological, biochemical and molecular parameters associated with either development, productivity, defense or in combination in untransformed (WT) and ProSys overexpressing (ProSys-OE) tomato plants. Thus, the chlorophyll fluorescence data obtained from plants grown under greenhouse experiments indicated that photosynthetic performance was not affected in ProSys-OE plants which also grew 7–14% taller than WT plants. Moreover, they showed accelerated flowering and yielded fruits of increased size (7–16% taller and wider) and weight (16–58% heavier), with modified fruit quality in terms of firmness (28% higher), titratable acidity (27–32% higher) and chemical composition. These findings suggest two complementary possibilities: (i) systemin is able to modulate both the wound response and plant development through the activation of jasmonic acid biosynthesis and signaling, and (ii) ProSys, an intrinsically disordered protein, acts as a signaling hub to regulate development and defense programs. These results shed light on the understanding of this plant regulatory mechanism and further suggest that systemin/ProSys-based regulation is central to control the defense-development balance in tomato. This knowledge could eventually lead to improved and more environmentally sound agricultural production practices.

scholarly journals Powdery mildew of Allium species caused by Oidiopsis taurica in Brazil

2004 ◽  
Vol 22 (4) ◽  
pp. 758-760 ◽  
Author(s):  
Ailton Reis ◽  
Leonardo S. Boiteux ◽  
Milton L. Paz-Lima ◽  
Patrícia P. Silva ◽  
Carlos A. Lopes
Keyword(s):  
Powdery Mildew ◽  
Allium Sativum ◽  
Leaf Surface ◽  
Federal District ◽  
Sweet Pepper ◽  
Host Specialization ◽  
Vegetable Crops ◽  
Allium Species ◽  
Powdery Mildew Disease ◽  
Fungal Colony

Oidiopsis taurica Salmon (Syn. Oidiopsis sicula Scalia) was identified as the causal agent of a powdery mildew disease occurring on distinct Allium species in Brazil. This disease was initially observed in plastic house and field-grown garlic (Allium sativum) and leek (A. porrum) accessions in Brasília (Federal District) and in field-grown and greenhouse onion (A. cepa) cultivars in Belém do São Francisco (Pernambuco State) and Brasília, respectively. Typical symptoms consisted of chlorotic areas on the leaf surface corresponding to a fungal colony. These lesions turned to a brownish color with the progress of the disease. Fungi morphology was similar to that described for O. taurica. Endophytic mycelium emerging through estomata, light pale conidia were dimorphic (lanceolate primary conidia and somewhat cylindrical secondary conidia), fibrosin bodies were absent, conidia formed predominantly single (not in chains), and appressoria were non-lobed. Its sexual stage, Leveillula taurica (Lev.) Arnaud, was not observed. Inoculations were performed with the O. taurica isolates from distinct Allium hosts. These isolates were also pathogenic to sweet pepper and tomato, indicating an apparent absence of host specialization. One bunching onion (A. fistulosum) accessions was not infected by O. taurica suggesting that this species might carry useful resistance alleles to this pathogen. This is the first formal report of a powdery mildew disease on species of the genus Allium in Brazil. This disease might become important on these vegetable crops especially in hot and dry areas such as those in the Central and Northeast regions of Brazil.

scholarly journals Stachybotriaceae on Cucurbits Demystified: Genetic Diversity and Pathogenicity of Ink Spot Pathogens

Plant Disease ◽  
2020 ◽  
Vol 104 (8) ◽  
pp. 2242-2251
Author(s):  
Gabriel Rennberger ◽  
Anthony P. Keinath
Keyword(s):  
South Carolina ◽  
Leaf Spot ◽  
Sequence Data ◽  
Spatial Scales ◽  
Fungal Species ◽  
Vegetable Crops ◽  
Tomato Plants ◽  
Predominant Species ◽  
The Family ◽  
The Common

Recently, the incidence of Myrothecium leaf spot, a foliar disease of watermelon, has increased in South Carolina. However, the identity of the fungal species responsible for outbreaks of this disease has not been determined. Sequence data from four partial gene regions were used to conduct Bayesian inference in order to identify 95 isolates of Stachybotriaceae. Isolates were collected in South Carolina between July 2015 and May 2018. In total, six species of Stachybotriaceae were identified on watermelon and two other cucurbits: Albifimbria verrucaria, Gregatothecium humicola, Paramyrothecium foliicola, P. humicola, Xenomyrothecium tongaense, and Xepicula leucotricha. Two species, G. humicola and P. foliicola, were the predominant species found. Within these two species, genetic differences within small spatial scales were detected. Five species (all except Xenomyrothecium tongaense) were tested in experiments to determine their pathogenicity and relative virulence on three hosts grown in rotation in South Carolina. Southern pea plants were less susceptible than watermelon and tomato plants, which were equally susceptible. This constitutes the first reliable report of pathogenicity of any of the five tested species of Stachybotriaceae on these three vegetable crops. Another important finding was that none of the isolates were identified as P. roridum, the species considered to be the only causal agent of Myrothecium leaf spot on cucurbits. We propose the common name “ink spot” for the foliar phase of diseases caused by genera within the family Stachybotriaceae. This name is descriptive and likely to be accepted by growers. To prevent further loss incurred by ink spot, watermelon and tomato crops should be monitored for this disease.

scholarly journals Sulfite Oxidase Activity Level Determines the Sulfite Toxicity Effect in Leaves and Fruits of Tomato Plants

Agronomy ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 694
Author(s):  
Umanath Sharma ◽  
Aizat Bekturova ◽  
Yvonne Ventura ◽  
Moshe Sagi
Keyword(s):  
Sulfite Oxidase ◽  
Activity Level ◽  
Vegetable Crops ◽  
Plant Tolerance ◽  
Wild Type ◽  
Biotic And Abiotic Stresses ◽  
Tomato Plants ◽  
Leaf Discs ◽  
Toxicity Effect ◽  
Treated Leaf

Increasing plant tolerance to sulfites/SO2 can lead to the development of tolerant crops to biotic and abiotic stresses. Plant sulfite oxidase (SO) is a molybdo-enzyme that oxidizes excess SO2/sulfite into non-toxic sulfate. The effect of toxic sulfite on leaves and fruits was studied in tomato plants with different SO expression: wild-type, SO overexpression (OE) and SO RNA interference (Ri). Sulfite-dipped ripe-fruits and sulfite treated leaf discs of Ri plants impaired in SO activity were more susceptible, whereas OE plants were more resistant, as revealed by remaining chlorophyll and tissue damage levels. Application of molybdenum further enhanced the tolerance of leaf discs to sulfite by enhancing SO activity in OE lines, but not in wild-type or Ri plants. Notably, incubation with tungsten, the molybdenum antagonist, overturned the effect of molybdenum spray in OE plants, revealed by remaining chlorophyll content and SO activity. The results indicate that SO in tomato leaves and ripe fruits determines the resistance to sulfite and the application of molybdenum enhances sulfite resistance in OE plants by increasing SO activity. Overall, the results suggest that SO overexpression can be employed, with or without molybdenum application, for developing fruit and vegetable crops tolerant to sulfite/SO2 containing pre- and postharvest treatments.

Changes in photosynthetic parameters and antioxidant activities following heat-shock treatment in tomato plants

2006 ◽  
Vol 33 (2) ◽  
pp. 177 ◽  
Author(s):  
Daymi Camejo ◽  
Ana Jiménez ◽  
Juan José Alarcón ◽  
Walfredo Torres ◽  
Juana María Gómez ◽  
...  
Keyword(s):  
Heat Shock ◽  
Antioxidant Activities ◽  
Dark Respiration ◽  
Photosynthetic Apparatus ◽  
Photosynthetic Parameters ◽  
Tomato Plants ◽  
Shock Temperature ◽  
Oxidative Processes ◽  
Gas Exchange Characteristics ◽  
Chlorophyll Photooxidation

Seedlings of two tomato genotypes, Lycopersicon esculentum Mill. var. Amalia and the wild thermotolerant type Nagcarlang, were grown under a photoperiod of 16 h light at 25°C and 8 h dark at 20°C. At the fourth true leaf stage, a group of plants were exposed to a heat-shock temperature of 45°C for 3 h, and measurements of chlorophyll fluorescence, gas-exchange characteristics, dark respiration and oxidative and antioxidative parameters were made after releasing the stress. The heat shock induced severe alterations in the photosynthesis of Amalia that seem to mitigate the damaging impact of high temperatures by lowering the leaf temperature and maintaining stomatal conductance and more efficient maintenance of antioxidant capacity, including ascorbate and glutathione levels. These effects were not evident in Nagcarlang. In Amalia plants, a larger increase in dark respiration also occurred in response to heat shock and the rates of the oxidative processes were higher than in Nagcarlang. This suggests that heat injury in Amalia may involve chlorophyll photooxidation mediated by activated oxygen species (AOS) and more severe alterations in the photosynthetic apparatus. All these changes could be related to the more dramatic effect of heat shock seen in Amalia than in Nagcarlang plants.

scholarly journals ЭФФЕКТИВНОСТЬ НЕКОРНЕВОЙ ПОДКОРМКИ МИКРОЭЛЕМЕНТНЫМИ УДОБРЕНИЯМИ НА УРОЖАЙНОСТЬ И КАЧЕСТВО ЗЕРНА ЯРОВОЙ ТРИТИКАЛЕ

2020 ◽  
Author(s):  
A.N. Kshnikatkina ◽  
A.N. Dolzhenko
Keyword(s):  
Grain Yield ◽  
Grain Quality ◽  
Leaf Surface ◽  
Technological Properties ◽  
Net Productivity ◽  
Maximum Value ◽  
Spring Triticale ◽  
Photosynthetic Potential ◽  
Grain Unit

Представлены результаты исследований по влиянию микроэлементных удобрений на формирование урожайности и качество зерна яровой тритикале сорта Укро. Установлено, что микроэлементные удобрения Азосол 36 Экстра, Мегамикс-Азот, Мегамикс-Профи, Цитовит и НаноКремний обеспечили увеличение параметров фотосинтеза агроценозов яровой тритикале. Максимальное значение листовой поверхности 42,5 тыс. м2/га, фотосинтетического потенциала 1,03 млн. м2 дн./га, чистой продуктивности фотосинтеза 6,29 г/м2 в сутки отмечены при двукратной фолиарной подкормке в фазы кущения и колошения препаратом Азосол 36 Экстра. Вследствие применения некорневой подкормки растений микроэлементными удобрениями в хелатной форме наблюдалось повышение урожайности яровой тритикале сорта Укро в совокупности с улучшением технологических свойств зерна. По вариантам опыта, в среднем за три года, урожайность зерна была 3,84-4,43 т/га. Прибавка по отношению к контролю колебалась в пределах 0,42-1,27 т/га (13,3-40,3). Наибольшая урожайность высококачественного зерна 4,43 т/га получена при двукратной подкормке в фазы кущения и колошения препаратом Азосол 36 Экстра, прибавка урожая зерна 1,27 т/га (40,3), натура зерна 796 г/л, стекловидность 63, сырая клейковина 26,2, белка 15,1.The results of studies on the influence of microelement fertilizers on the formation of productivity and grain quality of spring triticale of the Ukro variety are presented. It was found that microelement fertilizers Azosol 36 Extra, Megamix-Azot, Megamix-Profi, Tsitovit and NanoKremny provided an increase in the parameters of photosynthesis of agrocenoses of spring triticale.The maximum value of the leaf surface of 42.5 thousand m2/ha, the photosynthetic potential of 1.03 million m2 d/ ha, the net productivity of photosynthesis of 6.29 g/m2 per day were noted with double foliar dressing during the tillering and heading phases with Azosol 36 Extra. Due to the application of foliar dressing with microelement fertilizers in chelated form, an increase in the yield of spring triticale of the Ukro variety was observed, together with an improvement in the technological properties of grain. According to the experimental variants, on average for three years, the grain yield was 3.84-4.43 t/ha. The increase in relation to the control ranged from 0.42-1.27 t/ha (13.3-40.3). The highest yield of high-quality grain of 4.43 t/ha was obtained by double top dressing at the tillering and heading phases with the use of Azosol 36 Extra an increase in grain yield was 1.27 t/ha (40.3), grain unit - 796 g/l, glassiness - 63, raw gluten - 26.2, protein - 15.1.

scholarly journals Role of the HSP70 Homologue from Chloroplasts in the Assembly of the Photosynthetic Apparatus

1993 ◽  
Author(s):  
Rachel Nechushtai ◽  
Parag Chitnis
Keyword(s):  
Protein Complexes ◽  
Photosynthetic Apparatus ◽  
Crop Productivity ◽  
Cdna Clones ◽  
Light Harvesting Complex ◽  
Complex Protein ◽  
Chlorophyll Protein Complexes ◽  
Chlorophyll Protein

The major goal of the proposed research was to study the role of a 70-kDa heat shock cognate protein from chloroplasts (ct-HSP70) in the assembly of chlorophyll-protein complexes. The latters are mostly important in allowing photosynthesis to occur. Photosynthesis is at the heart of crop productivity and the knowledge of the biogenesis of the photosynthetic apparatus is essential to manipulate the efficiency of photosynthesis. The characterization of the function of the ct-HSP70 was planned to be studied in vitro by assaying its capability to physically interact with the thylakoid proteins and to assist their assembly into thylakoid membranes. We planned to identify regions in the light-harvesting complex protein (LHCP) that interact with the ct-HSP70 and characterize the interaction between them. We also intended to isolate cDNA clones encoding ct-HSP70, sequence them, express one of them in E. coli and use the purified protein for functional assays. The research in this BARD proposal aimed at providing insights and aid in understanding the mechanism by which plants may respond to the heat stress. Since plants often experience increased temperatures.

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