scholarly journals Features of morphogenesis, accumulation and redistribution of assimilate and nitrogen containing compounds in tomatoes under retardants treatment

2018 ◽  
Vol 8 (1) ◽  
pp. 356-362 ◽  
Author(s):  
V. G. Kuryata ◽  
O. O. Kravets
Keyword(s):  
Morphological Changes ◽  
Crop Productivity ◽  
Tomato Crop ◽  
Tomato Plants ◽  
Vegetative Organs ◽  
Palisade And Spongy Parenchyma ◽  
Source Sink ◽  
Sphere Formation ◽  
Nitrogen Containing Compounds

<p>We studied the influence of ethylene product Esphon® and triazole derivative Folicur® on the morphogenesis and productivity of tomatoes. The obtained results explained the significant role of morphological and mesostructural components of “source-sink” relation system of tomatoes in the formation of crop productivity. We established that the Folicur application resulted in more significant anatomical and morphological changes in the formation of leaf apparatus in comparison with the ethylene producer: the measurement of number, weight, area of leaf surface, and leaf index were higher in this variant of experiment. We also noted the appropriate changes in the mesostructure measurement of leaves: thickness of leave – main photosynthetic tissue of chlorenchyma, assimilatory cells size of palisade and spongy parenchyma. Analysis of depositing possibilities of plants vegetative organs at the fruitification stage indicates the importance of temporary postponement of nonstructural carbohydrates and nitrogen containing compounds in them followed by reutilization for carpogenesis needs. Application of Folicur resulted on the formation of a more powerful donor sphere and in the early fruit growth and formation stages are postponed more carbohydrates in vegetative organs of tomato plants compared to control. The content of sugars and starch in the roots, stems and leaves of plants under Folicur treatment was highest in all organs of the plant throughout the fruitification phase compared to control and variant with Esphon application. Our results also testify to the possibilities of nitrogen compounds remobilize from vegetative organs to carpogenesis needs. After Folicur treatment, the most intense decrease in the content of nitrogen containing compounds was observed during the transition from the fruit formation stage to green fruits stage in roots and stem of plant during the period of the most intense tomatoes growth. We concluded that a significant increase the yield of tomato crop was caused by the more powerful donor sphere formation and reutilization of assimilates and nitrogen containing compounds after Folicur application.</p>

scholarly journals Experimental evidence pointing to rain as a reservoir of tomato phyllosphere microbiota

2021 ◽  
Author(s):  
Marco Enrique Mechan Llontop ◽  
Long Tian ◽  
Parul Sharma ◽  
Logan Heflin ◽  
Vivian Angelica Bernal Galeano ◽  
...  
Keyword(s):  
Relative Abundance ◽  
Microbial Population ◽  
Amplicon Sequencing ◽  
Crop Productivity ◽  
Sterile Water ◽  
Tomato Plants ◽  
Operational Taxonomic Units ◽  
16S Rrna Amplicon Sequencing ◽  
Plant Microbiota

Plant microbiota play essential roles in plant health and crop productivity. Comparisons of community composition have suggested seeds, soil, and the atmosphere as reservoirs of phyllosphere microbiota. After finding that leaves of tomato (Solanum lycopersicum) plants exposed to rain carried a higher microbial population size than leaves of tomato plants not exposed to rain, we experimentally tested the hypothesis that rain is a so far neglected reservoir of phyllosphere microbiota. Rain microbiota were thus compared with phyllosphere microbiota of tomato plants either treated with concentrated rain microbiota, filter-sterilized rain, or sterile water. Based on 16S rRNA amplicon sequencing, one-hundred and four operational taxonomic units (OTUs) significantly increased in relative abundance after inoculation with concentrated rain microbiota but no OTU significantly increased after treatment with either sterile water or filter-sterilized rain. Some of the genera to which these 104 OTUs belonged were also found at higher relative abundance on tomatoes exposed to rain outdoors than on tomatoes grown protected from rain in a commercial greenhouse. Taken together, these results point to precipitation as a reservoir of phyllosphere microbiota and show the potential of controlled experiments to investigate the role of different reservoirs in the assembly of phyllosphere microbiota.

scholarly journals Pseudomonas corrugata and Pseudomonas marginalis associated with the collapse of tomato plants in rockwool slab hydroponic culture

2010 ◽  
Vol 46 (No. 1) ◽  
pp. 1-11 ◽  
Author(s):  
V. Kůdela ◽  
V. Krejzar ◽  
I. Pánková
Keyword(s):  
Czech Republic ◽  
Pathogenic Bacteria ◽  
Soft Rot ◽  
Hydroponic Culture ◽  
Hypodermic Needle ◽  
Pseudomonas Corrugata ◽  
Tomato Crop ◽  
Tomato Plants ◽  
Vascular Pathogens

Plant pathogenic species Pseudomonas corrugata and P. marginalis were detected and determined in collapsed tomato plants in rockwool slab hydroponic culture in southern Moravia, Czech Republic. Surprisingly, P. marginalis was also determined before planting in apparently healthy grafted tomato transplants grown in hydroponic culture. Moreover, non-pathogenic P. fluorescens, P. putida, P. synxantha, and Stenotrophomonas malthophilia were identified. The Biolog Identification GN2&nbsp;MicroPlate&trade; System System (Biolog, Inc., Hayward, USA) was used for identification of bacterial isolates. Cultures of P. corrugata and P. marginalis were used in a greenhouse pathogenicity experiment. Seven weeks old tomato plants of cv. Moneymaker grown in sterilised perlite were inoculated into the stem with a hypodermic needle at one point above the cotyledon node. In inoculated tomato plants, disease symptoms were observed that included external and internal dark brown lesions around the inoculation site, watering and collapse of pith and sometimes also vascular browning and wilting of leaves. In comparison with P. marginalis, P. corrugata appeared to be a much stronger pathogen. Both tested Pseudomonas species were recovered from inoculated tomato plants. P. corrugata was found to move both upwards to the apex of the stem and downwards from the site of the inoculated stem into roots. When inoculated into potato tuber slices, some tomato strains of P. marginalis, P. fluorescens, P. synxantha, and Pseudomonas sp. produced soft rot. However, other strains of the same species were not able to macerate the potato tissue. It is concluded that P. corrugata and P. marginalis can be associated with the collapse of tomato crop in soilless culture grown in a greenhouse. This is the first report on P. corrugata in tomato plants in the Czech Republic. The role of plant pathogenic bacteria, fungal root rot and vascular pathogens and Pepino mosaic virus in the collapse of tomato plants is discussed.

scholarly journals Impact of gibberelic acid and tebuconazole on formation of the leaf system and functioning of donor – acceptor plant system of solanaceae vegetable crops

2017 ◽  
Vol 8 (2) ◽  
pp. 162-168 ◽  
Author(s):  
V. H. Kuryata ◽  
V. V. Rogach ◽  
O. I. Buina ◽  
O. V. Kushnir ◽  
O. V. Buinyi
Keyword(s):  
Mass Fraction ◽  
Leaf Surface ◽  
Photosynthetic Apparatus ◽  
Crop Productivity ◽  
Vegetable Crops ◽  
Tomato Plants ◽  
Vegetative Organs ◽  
Net Productivity ◽  
Donor Acceptor ◽  
Gibberelic Acid

We studied the comparable effect of gibberelic acid and tebuconazole on morphogenesis, mesostructure formation and redistribution of flows in sweet peppers and tomatoes. It has been found that the use of gibberelic acid and tebuconazole retardant during budding leads to increased plant productivity due to optimization of the structure and operation of the plants’ leaf apparatus. It was established that both gibberelic and antigibberelic tebuconazole drug stimulated the formation and functioning of the photosynthetic apparatus of peppers and tomatoes, but the mechanisms of this regulation were different. Increased photosynthetic activity of plants under the influence of gibberellin was determined primarily by the formation of more leaves and total leaf surface. When using tebuconazole retardant there was a significant restructuring of the organization of leaf mezostructure: the leaves were thickened by chlorenchyma proliferation, there was an increase in the volume of columnar parenchyma cells and linear dimensions of spongy parenchyma leaf cells. The surface density of leaves significantly increased, the chlorophyll content and nitrogen content (especially protein) also increased, compared with control variants and variants using gibberelin. Such a profound restructuring of the photosynthetic apparatus in plants under the actions of tebuconazole led to a significant increase in donor leaves function of peppers and tomatoes, which is an indicator of the growth of net productivity of photosynthesis – the highest among all the variants of the experiment. The results also show that increasing the chlorophyll phytocenotic index was more significant than the increase of leaf index: the tomatoes under the action of tebuconazole had a lower leaf index than in control options, but due to a higher chlorophyll index the crop productivity increased.Since during the fruiting period the costs of assimilates to the growth of vegetative organs are greatly reduced, optimization of photosynthetic apparatus in pepper and tomato plants led to the laying of more fruit per plant and increasing crop yield. The analysis of the mass ratio of the researched vegetative and fruit plants shows that the mass fraction of fruit (an acceptor sphere of plants during fruiting) under the action of both drugs increased. Thus in both variants of the experiment both the mass fraction and donor assimilates of leaves were higher. Apart from the main source of assimilates – the processes of photosynthesis, which intensified due to the formation of a larger area of leaf surface (variant with gibberelin) or optimization of mesostructure (variant with tebuconazole) it is probable that the formation and growth of the embryo occurred in part due to reutilization of carbohydrates from the vegetative plant organs in carpogenesis processes. 

scholarly journals Experimental evidence pointing to rain as a reservoir of tomato phyllosphere microbiota

2021 ◽  
Author(s):  
Marco E Mechan Llontop ◽  
Long Tian ◽  
Parul Sharma ◽  
Logan Heflin ◽  
Vivian A Bernal-Galeano ◽  
...  
Keyword(s):  
Relative Abundance ◽  
Microbial Population ◽  
Amplicon Sequencing ◽  
Crop Productivity ◽  
Sterile Water ◽  
Tomato Plants ◽  
Operational Taxonomic Units ◽  
16S Rrna Amplicon Sequencing ◽  
Plant Microbiota

Plant microbiota play essential roles in plant health and crop productivity. Comparisons of community composition have suggested seeds, soil, and the atmosphere as reservoirs of phyllosphere microbiota. After finding that leaves of tomato (Solanum lycopersicum) plants exposed to rain carried a higher microbial population size than leaves of tomato plants not exposed to rain, we experimentally tested the hypothesis that rain is a so far neglected reservoir of phyllosphere microbiota. Rain microbiota were thus compared with phyllosphere microbiota of tomato plants either treated with concentrated rain microbiota, filter-sterilized rain, or sterile water. Based on 16S rRNA amplicon sequencing, one-hundred and four operational taxonomic units (OTUs) significantly increased in relative abundance after inoculation with concentrated rain microbiota but no OTU significantly increased after treatment with either sterile water or filter-sterilized rain. Some of the genera to which these 104 OTUs belonged were also found at higher relative abundance on tomatoes exposed to rain outdoors than on tomatoes grown protected from rain in a commercial greenhouse. Taken together, these results point to precipitation as a reservoir of phyllosphere microbiota and show the potential of controlled experiments to investigate the role of different reservoirs in the assembly of phyllosphere microbiota.

scholarly journals Особливості функціонування донорно-акцепторної системи маку олійного за дії трептолему в зв’язку з продуктивністю культури

2018 ◽  
Vol 8 (1) ◽  
pp. 11-20 ◽  
Author(s):  
V. G. Kuryata ◽  
S. V. Polyvanyi
Keyword(s):  
Crop Yields ◽  
Morphological Changes ◽  
Morphological Structure ◽  
Nonstructural Carbohydrates ◽  
Practical Result ◽  
Vegetative Organs ◽  
Growth Stimulator ◽  
Treated Plant ◽  
Phosphorus And Potassium ◽  
Source Sink

<p>We studied the action of synthetic growth stimulator (treptolem) on the morphogenesis, leaf mesostructure, redistribution of assimilates, and nutrients between organs of oil poppy (<em>Papaver somniferum</em> <em>L.</em>) in ontogenesis, productivity and structure of crop. Optimization of production process of oil poppy under the influence of growth stimulator is realized through the source-sink relation system of the plant. Obtained results testify the essential role of morphological and mesostructural components in regulation of source-sink relation system of oil poppy. Appplcation of treptolem’s growth stimulating contributed the formation of more powerful donor sphere and increased the number and mass of leaves, the area of leaf surface and the leaf index. It was found that the reason of such morphological changes caused significant increase in branching of the stem by the action of treatment. The intensification of donor function poppy plants by treptolem also occurred as a result of mesostructural changes in the leaves. Treptolem application induced formation of a more powerful layer of chlorenchymes - the main photosynthetic tissues of plant, linear sizes of chlorenchymal cells and chlorophylls content increased in them. In that case, the intensification of growth processes at the beginning of vegetation under the influence of preparation led to forme a more powerful donor potential of the plant per unit of cenosis area, which created prerequisites to increase the productivity of culture. It was found that the consequence of such changes was an increase of the content of nonstructural carbohydrates (sugar + starch) in vegetative organs of treated variant in compared with the control of intense growth. Treatment showed apparent branching of the stems, that induced a larger number of flowers and fruitcases were laid an increase of acceptor capacity of plant. The result of these changes was determined by more intensive flow of nonstructural carbohydrates on the formation and growth of fruits in the variant with treptolem. In the flowering phase in the roots and leaves of plants, it was observed the highest content of nitrogen that during the formation and growth of the fruit was remobilized on the carpogenesis process. At the same time, the more intensively use of nitrogen by the vegetative organs of the poppy plants to form the fruitcases was in the variant with treptolem. It was noted the lower levels of phosphorus in the leaves and potassium in the vegetative organs of oil poppy in the variant of treptolem treated plant. It testifies that preparation treatment contributed to the more intensive reutilisation of nitrogen, phosphorus, and potassium compounds in fruit formation. The intensified flow of assimilates and nitrogen-containing compounds together with nutrients to a greater number of implanted fruits provided the growth of crop yields (by increase in the number of cases, the mass of seeds in case, and the mass of a single seed). It is confirmed that there is negative correlation between accumulation of oil and content of nitrogen. It was established that application of treptolem increased the content of unsaturated higher fatty acids in oil poppy. An important practical result of the research should be considered that treptolem treatment increased the content of alkaloids in the seeds of poppy plants, which could e useful in pharmacological industry. We proved that treptolem application during the budding period on the poppy seed leads to increase the yield of crop due to optimized morphological structure of plant, the mesosurface structure of leaf; it is also increased the potential trapping due to the laying and formation of a greater number of fruits.</p>

scholarly journals Role of endocannabinoid CB1 receptors in Streptozotocin-induced uninephrectomised Wistar rats in diabetic nephropathy

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Jayarami Reddy Medapati ◽  
Deepthi Rapaka ◽  
Veera Raghavulu Bitra ◽  
Santhosh Kumar Ranajit ◽  
Girija Sankar Guntuku ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Morphological Changes ◽  
Serum Levels ◽  
Cb1 Receptors ◽  
Protective Effects ◽  
Renal Hypertrophy ◽  
Necrosis Factor Alpha

Abstract Background The endocannabinoid CB1 receptor is known to have protective effects in kidney disease. The aim of the present study is to evaluate the potential agonistic and antagonistic actions and to determine the renoprotective potential of CB1 receptors in diabetic nephropathy. The present work investigates the possible role of CB1 receptors in the pathogenesis of diabetes-induced nephropathy. Streptozotocin (STZ) (55 mg/kg, i.p., once) is administered to uninephrectomised rats for induction of experimental diabetes mellitus. The CB1 agonist (oleamide) and CB1 antagonist (AM6545) treatment were initiated in diabetic rats after 1 week of STZ administration and were given for 24 weeks. Results The progress in diabetic nephropathy is estimated biochemically by measuring serum creatinine (1.28±0.03) (p < 0.005), blood urea nitrogen (67.6± 2.10) (p < 0.001), urinary microprotein (74.62± 3.47) (p < 0.005) and urinary albuminuria (28.31±1.17) (p < 0.0001). Renal inflammation was assessed by estimating serum levels of tumor necrosis factor alpha (75.69±1.51) (p < 0.001) and transforming growth factor beta (8.73±0.31) (p < 0.001). Renal morphological changes were assessed by estimating renal hypertrophy (7.38± 0.26) (p < 0.005) and renal collagen content (10.42± 0.48) (p < 0.001). Conclusions From the above findings, it can be said that diabetes-induced nephropathy may be associated with overexpression of CB1 receptors and blockade of CB1 receptors might be beneficial in ameliorating the diabetes-induced nephropathy. Graphical abstract

scholarly journals Light Emitting Diodes (LEDs) as Agricultural Lighting: Impact and Its Potential on Improving Physiology, Flowering, and Secondary Metabolites of Crops

2021 ◽  
Vol 13 (4) ◽  
pp. 1985
Author(s):  
Musa Al Murad ◽  
Kaukab Razi ◽  
Byoung Ryong Jeong ◽  
Prakash Muthu Arjuna Samy ◽  
Sowbiya Muneer
Keyword(s):  
Crop Production ◽  
Morphological Changes ◽  
Light Emitting Diode ◽  
Crop Productivity ◽  
Photon Flux ◽  
Flower Initiation ◽  
Nutrient Metabolism ◽  
Light Emitting ◽  
Cultivation Systems ◽  
Lighting Systems

A reduction in crop productivity in cultivable land and challenging environmental factors have directed advancement in indoor cultivation systems, such that the yield parameters are higher in outdoor cultivation systems. In wake of this situation, light emitting diode (LED) lighting has proved to be promising in the field of agricultural lighting. Properties such as energy efficiency, long lifetime, photon flux efficacy and flexibility in application make LEDs better suited for future agricultural lighting systems over traditional lighting systems. Different LED spectrums have varied effects on the morphogenesis and photosynthetic responses in plants. LEDs have a profound effect on plant growth and development and also control key physiological processes such as phototropism, the immigration of chloroplasts, day/night period control and the opening/closing of stomata. Moreover, the synthesis of bioactive compounds and antioxidants on exposure to LED spectrum also provides information on the possible regulation of antioxidative defense genes to protect the cells from oxidative damage. Similarly, LEDs are also seen to escalate the nutrient metabolism in plants and flower initiation, thus improving the quality of the crops as well. However, the complete management of the irradiance and wavelength is the key to maximize the economic efficacy of crop production, quality, and the nutrition potential of plants grown in controlled environments. This review aims to summarize the various advancements made in the area of LED technology in agriculture, focusing on key processes such as morphological changes, photosynthetic activity, nutrient metabolism, antioxidant capacity and flowering in plants. Emphasis is also made on the variation in activities of different LED spectra between different plant species. In addition, research gaps and future perspectives are also discussed of this emerging multidisciplinary field of research and its development.

scholarly journals The Role of Source‐sink Dynamics in the Assessment of Risk to Non‐target Arthropods from the Use of Plant Protection Products

2021 ◽  
Author(s):  
Gavin Lewis ◽  
Axel Dinter ◽  
Charlotte Elston ◽  
Michael Thomas Marx ◽  
Christoph Julian Mayer ◽  
...  
Keyword(s):  
Plant Protection ◽  
Plant Protection Products ◽  
Source Sink

Tissue Ki67 proliferative index expression and pathological changes in hemodialysis arteriovenous fistulae: Preliminary single-center results

2021 ◽  
pp. 112972982110154
Author(s):  
Raffaella Mauro ◽  
Cristina Rocchi ◽  
Francesco Vasuri ◽  
Alessia Pini ◽  
Anna Laura Croci Chiocchini ◽  
...  
Keyword(s):  
Morphological Changes ◽  
Hot Spot ◽  
Wall Thickening ◽  
Proliferating Cells ◽  
Ki 67 ◽  
Group A ◽  
The Mean ◽  
Set Up ◽  
Group B

Background: Arteriovenous fistula (AVF) for hemodialysis integrates outward remodeling with vessel wall thickening in response to drastic hemodynamic changes. Aim of this study is to determine the role of Ki67, a well-established proliferative marker, related to AVF, and its relationship with time-dependent histological morphologic changes. Materials and methods: All patients were enrolled in 1 year and stratified in two groups: (A) pre-dialysis patients submitted to first AVF and (B) patients submitted to revision of AVF. Morphological changes: neo-angiogenesis (NAG), myointimal thickening (MIT), inflammatory infiltrate (IT), and aneurysmatic fistula degeneration (AD). The time of AVF creation was recorded. A biopsy of native vein in Group A and of arterialized vein in Group B was submitted to histological and immunohistochemical (IHC) analysis. IHC for Ki67 was automatically performed in all specimens. Ki67 immunoreactivity was assessed as the mean number of positive cells on several high-power fields, counted in the hot spots. Results: A total of 138 patients were enrolled, 69 (50.0%) Group A and 69 (50.0%) Group B. No NAG or MIT were found in Group A. Seven (10.1%) Group A veins showed a mild MIT. Analyzing the Group B, a moderate-to-severe MIT was present in 35 (50.7%), IT in 19 (27.5%), NAG in 37 (53.6%); AD was present in 10 (14.5%). All AVF of Group B with the exception of one (1.4%) showed a positivity for Ki67, with a mean of 12.31 ± 13.79 positive cells/hot spot (range 0–65). Ki67-immunoreactive cells had a subendothelial localization in 23 (33.3%) cases, a myointimal localization in SMC in 35 (50.7%) cases. The number of positive cells was significantly correlated with subendothelial localization of Ki67 ( p = 0.001) and with NA ( p = 0.001). Conclusions: Native veins do not contain cycling cells. In contrast, vascular cell proliferation starts immediately after AVF creation and persists independently of the time the fistula is set up. The amount of proliferating cells is significantly associated with MIT and subendothelial localization of Ki67-immunoreactive cells, thus suggesting a role of Ki-67 index in predicting AVF failure.

scholarly journals Long non-coding RNA LINC00665 promotes gemcitabine resistance of Cholangiocarcinoma cells via regulating EMT and stemness properties through miR-424-5p/BCL9L axis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Min Lu ◽  
Xinglei Qin ◽  
Yajun Zhou ◽  
Gang Li ◽  
Zhaoyang Liu ◽  
...  
Keyword(s):  
Acquired Resistance ◽  
Transcriptional Regulators ◽  
First Line ◽  
Protein Coding ◽  
Gemcitabine Resistance ◽  
Non Coding Rna ◽  
Sphere Formation ◽  
Long Non Coding Rna ◽  
Line Chemotherapy

AbstractGemcitabine is the first-line chemotherapy drug for cholangiocarcinoma (CCA), but acquired resistance has been frequently observed in CCA patients. To search for potential long noncoding RNAs (lncRNAs) involved in gemcitabine resistance, two gemcitabine resistant CCA cell lines were established and dysregulated lncRNAs were identified by lncRNA microarray. Long intergenic non-protein coding RNA 665 (LINC00665) were found to rank the top 10 upregulated lncRNAs in our study, and high LINC00665 expression was closely associated with poor prognosis and chemoresistance of CCA patients. Silencing LINC00665 in gemcitabine resistant CCA cells impaired gemcitabine tolerance, while enforced LINC00665 expression increased gemcitabine resistance of sensitive CCA cells. The gemcitabine resistant CCA cells showed increased EMT and stemness properties, and silencing LINC00665 suppressed sphere formation, migration, invasion and expression of EMT and stemness markers. In addition, Wnt/β-Catenin signaling was activated in gemcitabine resistant CCA cells, but LINC00665 knockdown suppressed Wnt/β-Catenin activation. B-cell CLL/lymphoma 9-like (BCL9L), the nucleus transcriptional regulators of Wnt/β-Catenin signaling, plays a key role in the nucleus translocation of β-Catenin and promotes β-Catenin-dependent transcription. In our study, we found that LINC00665 regulated BCL9L expression by acting as a molecular sponge for miR-424-5p. Moreover, silencing BCL9L or miR-424-5p overexpression suppressed gemcitabine resistance, EMT, stemness and Wnt/β-Catenin activation in resistant CCA cells. In conclusion, our results disclosed the important role of LINC00665 in gemcitabine resistance of CCA cells, and provided a new biomarker or therapeutic target for CCA treament.

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