Keefektifan beberapa senyawa kimia sebagai agen penginduksi resistensi tanaman sawi terhadap penyakit bercak daun Curvularia

Penyakit bercak daun Curvularia merupakan penyakit baru pada tanaman sawi. Pengendalian penyakit dengan fungisida sintetik sangat berbahaya karena daun sawi dikonsumsi sebagai sayuran mentah atau pengolahan minimal, untuk itu diperlukan alternatif cara pengendalian yang lebih aman bagi kesehatan seperti menginduksi resistensi tanaman menggunakan bahan kimia. Penelitian ini bertujuan untuk mengetahui keefektifan beberapa bahan penginduksi resistensi seperti asam salisilat, kitin, K2HPO4, dan vitamin B1. Sementara, Bion M1/48, bahan penginduksi komersial, digunakan sebagai pembanding. Bahan penginduksi diaplikasikan melalui cara perendaman benih dan disemprotkan. Pada perlakuan perendaman, benih direndam dalam suspensi bahan penginduksi selama 12 jam, sedangkan pada perlakuan penyemprotan, bahan penginduksi disemprotkan satu kali yaitu dua hari sebelum inokulasi dilakukan. Inokulasi patogen yang digunakan memiliki suspensi konidia kerapatan 5 x 105 konidia ml-1. Penelitian ini menggunakan Rancangan Acak Kelompok dengan empat ulangan. Pengamatan dilakukan terhadap masa inkubasi dan intensitas penyakit. Hasil pengujian menunjukkan bahwa perendaman benih asam salisilat, kitin dan K2HPO4, mampu menunda kemunculan gejala sakit lebih lama dibandingkan Bion M 1/48. Terhadap intensitas penyakit, asam salisilat, kitin, dan K2HPO4, juga efektif menginduksi resistensi tanaman sawi terhadap penyakit bercak daun Curvularia. Ketiga senyawa kimia ini potensial untuk digunakan sebagai pengganti fungisida sintetis dalam mengendalikan penyakit bercak daun Curvularia pada tanaman sawi.ABSTRACTCurvularia leaf spot is a new disease on mustard green (Brassica juncea L.). The diseases control with synthetic fungicide is dangerous since mustard green leaves are freshly consumed or with minimum treatments. Therefore, a safer alternative method must be sought such inducing plant resistance using chemical inducers. This research aimed to evaluate effectiveness of salicylic acid, chitin, K2HPO4, vitamin B1, as resistance inducers. Meanwhile, Bion M 1/48, a commercial inducer, was used as the check treatment. These substances were applied as either seed soaking or spraying. In the soaking treatmet, the seeds were soaked for 12 hours duration, whereas in the spraying treatment, chemicals were applied once, at two days before inoculation with conidial suspension of 5 x 105 conidia ml-1. Experimental plants were arranged in a Randomized Block Design with four replicates. Observation was carried out on incubation period and disease intensity. Results showed that soaking the seed in salicylic acid, chitin and K2HPO4 was able to delay the appearance of symptoms, better than of Bion M 1/48. Towards disease intensity, salicylic acid, chitin, and K2HPO4 were also effective in inducing resistance of mustard green leaves against Curvularia leaf spot. The three substances were potential to be applied as an alternative to synthetic fungicide in managing Curvularia leaf spot on the mustard green.

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PENGARUH KOTORAN AYAM DAN PACLOBUTRAZOL TERHADAP JUMLAH ANAKAN DAN TINGGI BATANG PADI HITAM (Oryza sativa L.) KABUPATEN TAPANULI SELATAN

J-PEN Borneo : Jurnal Ilmu Pertanian ◽

10.35334/jpen.v2i3.1526 ◽

2020 ◽

Vol 2 (2) ◽

Author(s):

Romaya Sitha Silitonga ◽

Jumaria Nasution

Keyword(s):

Vitamin E ◽

Rice Plant ◽

Oryza Sativa L ◽

Block Design ◽

Vitamin B1 ◽

Brown Rice ◽

Tiller Number ◽

Chicken Manure ◽

Black Rice ◽

Randomized Block Design

ABSTRACT Black rice is very potential to be expanded because the content of anthocyanin, vitamin B1, and vitamin E black rice is higher than brown rice or white rice. At present, black rice producers are in Java, whereas in Sumatra, South Tapanuli District, Angkola Sub-District is a new type of black rice for rice farmers, so it is necessary to introduce and cultivate. The aims of the research was to determine the effect of chicken manure and paclobutrazol concentration on the tiller number and stems height of the rice plant in Batang Angkola District, South Tapanuli. This research method used was Randomized Block Design Factorial with two factors. The first factor was application of chicken manure (C) with dosage 0 g/pot 3 g/pot (C1), 6 g/pot (C2) and 9 g/pot (P3). The second factor was application paklobutrazol (P) with concentrations of 0 ppm (P0), 25 ppm (P1), 50 ppm (P2) and 75 ppm (P3). Statistical analysis used Anova 5%. If there is a difference followed by DMRT. The result showed that application of chicken manure at 9 g/pot (C3) and paclobutrazol 75 ppm (P3) were produce more tiller number and able to decrease height of black rice plant. Key words : Black Rice, Chicken manure, Paklobutrazol, South Tapanuli District, Tiller number, ABSTRAK Padi hitam sangat potensial dikembangkan karena kandungan antosianin, vitamin B1, dan vitamin E padi hitam lebih tinggi dari beras merah maupun beras putih. Adapun daerah penghasil beras hitam saat ini masih terdapat di daerah-daerah Pulau Jawa, sementara di Pulau Sumatera, khususnya di Kabupaten Tapanuli Selatan, Kecamatan Batang Angkola beras hitam masih tergolong tanaman padi jenis baru bagi para petani beras sehingga perlu dilakukan upaya perkenalan dan budidaya di daerah ini. Penelitian ini bertujuan mengetahui pengaruh konsentrasi kotoran ayam dan paclobutrazol terhadap jumlah anakan dan tinggi batang padi hitam yang tumbuh di Kecamatan Batang Angkola, Tapanuli Selatan. Metode penelitian yang digunakan adalah Rancangan Acak Kelompok Faktorial dengan 2 faktor. Faktor pertama, aplikasi Kotoran ayam (C) dengan dosis 0 g/pot (C0), 3 g/pot (C1), 6 g/pot (C2) dan 9 g/pot. Faktor kedua, paklobutrazol (P) dengan konsentrasi 0 ppm (P0), 25 ppm (P1), 50 ppm (P2) dan 75 ppm (P3). Data dianalisis menggunakan statistik analisis variansi pada taraf kesalahan 5%. Apabila ada beda nyata dilanjutkan dengan DMRT. Hasil penelitian aplikasi Kotoran ayam 9 g/pot (C3) dan paclobutrazol 75 ppm (P3) menghasilkan jumlah anakan lebih banyak dan dapat menekan pertumbuhan tinggi batang padi hitam yang ditanam. Kata Kunci : Jumlah anakan, Kabupaten Tapanuli Selatan, Kotoran Ayam, Paclobutrazol, Padi hitam.

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Calcium and potassium contents in nutrient solution on Phoma leaf spot intensity in coffee seedlings

Revista CERES ◽

10.1590/0034-737x201663040008 ◽

2016 ◽

Vol 63 (4) ◽

pp. 486-491 ◽

Cited By ~ 1

Author(s):

Aricléia de Moraes Catarino ◽

Edson Ampélio Pozza ◽

Adélia Aziz Alexandre Pozza ◽

Leone Stabile dias Santos ◽

Gabriel Brandão Vasco ◽

...

Keyword(s):

Nutrient Solution ◽

Leaf Spot ◽

Block Design ◽

Fungal Diseases ◽

Growth Chamber ◽

Spot Intensity ◽

Controlled Conditions ◽

Progress Curve ◽

Randomized Block Design ◽

Nutrient Solutions

ABSTRACT Coffee is one of the main export commodities of Brazilian agribusiness. Phoma leaf spot [Phoma tarda (Stewart) Boerema & Bollen] is one of the most important coffee fungal diseases in Brazil. Therefore, this study aimed to evaluate the intensity of Phoma leaf spot in coffee seedlings supplied with different rates of Ca+2 and K+. The study was conducted under controlled conditions in a growth chamber, at the Department of Phytopathology - UFLA, from February 2010 to December 2011. The assay was repeated twice under the same conditions. The nutrient solutions consisted of five concentrations of K+ (3, 4, 5, 6, 7 mmol L-1) and Ca+2 (2, 4, 6, 8 and 10 mmol L-1). The experiment was arranged in a randomized block design, with 25 treatments and three replicates, with two plants per plot. The areas under incidence progress curve (AUIPC) and severity (AUSPC) were calculated. At the lowest rate of Ca2+ (2 mmol L-1) and highest K+ (6 and 7 mmol L-1), approximately, the AUIPC was the smallest. For the AUSPC, the lowest rates of Ca+2 and K+ resulted in the lowest severities. Supply of Ca+2 and K+ in nutrient solution reduced AUIPC and AUSPC of Phoma leaf spot, and these nutrients can be recommended for the management of the disease.

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KEEFEKTIFAN Trichoderma sp. DAN Fusarium NON PATOGENIK DALAM MENGENDALIKAN PENYAKIT BUSUK PUCUK VANILI BERWAWASAN LINGKUNGAN / Effectiveness of Trichoderma sp. and non-pathogenic Fusarium to Environmentally Control Vanilla Shoot Rot Disease

Jurnal Penelitian Tanaman Industri ◽

10.21082/littri.v23n1.2017.18-25 ◽

2017 ◽

Vol 23 (1) ◽

pp. 18

Author(s):

Efi Taufiq ◽

Hasim Hasim ◽

Bonny PW Soekarno ◽

M. Surahman

Keyword(s):

Block Design ◽

Phytophthora Capsici ◽

Combine Application ◽

Conidial Suspension ◽

Randomized Block Design ◽

Disease Occurrence ◽

Trichoderma Sp ◽

Rot Disease ◽

The One ◽

Important Disease

ABSTRAKPenyakit busuk pucuk vanili (BPV) merupakan salah satu penyakit penting vanili yang berpotensi mengurangi produksi vanili di Indonesia. Penyakit BPV di Indonesia umumnya merusak pembibitan, namun akibat perubahan iklim yang ekstrim, serangan penyakit BPV pada tanaman vanili dewasa di kebun mengalami peningkatan. Penelitian dilakukan untuk mengetahui keefektifan Trichoderma sp. dan Fusarium non patogenik (FusNP) dalam mengendalikan penyakit BPV di kebun vanili. Penelitian lapangan dilakukan di KP Sukamulya, Sukabumi mulai November 2015 - Juli 2016. Pengujian residu fungisida sintetik dilakukan di Laboratorium Residu Bahan Agrokimia Balai Penelitian Lingkungan Pertanian, Laladon Bogor. Penelitian terdiri atas lima perlakuan yaitu pemberian substrat Trichoderma sp. (T), penyemprotan suspensi konidia FusNP (F), kombinasi Trichoderma sp. dan FusNP (TF), fungisida sintetik mancozeb (M) dan kontrol (K). Perlakuan disusun dalam rancangan acak kelompok (RAK), tiap perlakuan diulang sebanyak lima kali. Variabel yang diamati adalah gejala dan keparahan penyakit, curah hujan, dan residu fungisida sintetik. Data dianalisis dengan Uji Tukey pada selang kepercayaan 95%. Hasil penelitian menunjukkan bahwa Trichoderma sp. dan FusNP cukup efektif mengendalikan penyakit BPV di lapangan, setara dengan keefektifan fungisida sintetik yaitu kejadian penyakit berkisar 2 - 5%, sedangkan kontrol mencapai 32%. Perkembangan penyakit BPV dipengaruhi oleh curah hujan, semakin tinggi curah hujan semakin tinggi intensitas serangan penyakit BPV. Penggunaan fungisida sintetik secara intensif menyebabkan pencemaran lingkungan berupa residu pestisida pada daun, buah, dan tanah rizosfer vanili..Kata kunci: busuk pucuk vanili, Phytophthora. capsici, agens hayati. ABSTRACTVanilla shoot rot disease (VSR) is one of important disease that potentially reduces Indonesia’s vanilla production. The VSR disease is prevalently developing in the nursery, but due to the extreme climate change, the disease occurrence in the garden has increased recently. A present study was aimed to assess the effectiveness of Trichoderma sp. and non-pathogenic Fusarium (NPF) in controlling the VSR disease in the garden. An experiment was conducted in a vanilla garden at KP Sukamulya, Sukabumi November 2015 - July 2016. The study consisted of five treatments that were application of Trichoderma sp. substrate (T) onto the vanilla tips, spraying the conidial suspension of FusNP (F), a combine application of Trichoderma sp. and FusNP (TF), synthetic fungicide mancozeb (M) and the control (K). The treatments were arranged in a randomized block design, replicated five times each. The variables measured were the incidence and severity of VSR diseases monthly, residue of synthetic fungicides and rainfall. The results showed that application of Trichoderma sp. sp. and NPF reduced the disease severity of VSR 3 % and 5 % respectively than the one of control. While the fungicide application was 3% lower than the control. The VSR disease progress is affected significantly by rainfall period. Keyword: vanilla shoot rot disease, Phytophthora capsici. Bioagent.

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First Report of Fusarium ipomoeae Causing Peanut leaf Spot in China

Plant Disease ◽

10.1094/pdis-01-21-0226-pdn ◽

2021 ◽

Author(s):

Manlin Xu ◽

Xia Zhang ◽

Jing Yu ◽

zhiqing Guo ◽

Ying Li ◽

...

Keyword(s):

Leaf Spot ◽

Genomic Dna ◽

Block Design ◽

Management Strategies ◽

Morphological Characteristics ◽

Ethanol Solution ◽

Distilled Water ◽

Conidial Suspension ◽

Internal Transcribed Spacer Region ◽

First Report

Peanut (Arachis hypogaea L.) is one of the most economically important crops as an important source of edible oil and protein. In August 2020, circular to oval-shaped brown leaf spots (2-6 mm in diameter) with well-defined borders surrounded by a yellow margin were observed on peanut plant leaves in Laixi City, Shandong Province, China. Symptomatic plants randomly distributed in the field, the incidence was approximately 5%. Leave samples were collected consisted of diseased tissue and the adjacent healthy tissue. The samples were dipped in a 70% (v/v) ethanol solution for 30 s and then soaked in a 0.1% (w/v) mercuric chloride solution for 60 s. The surface-sterilized tissues were then rinsed three times with sterile distilled water, dried and placed on Czapek Dox agar supplemented with 100 μg/ml of chloramphenicol. The cultures were incubated in darkness at 25 °C for 3–5 days. Fungal colonies were initially white and radial, turning to orange-brown in color, with abundant aerial mycelia. Macroconidia were abundant, 4 to 7 septate, with a dorsiventral curvature, and were 3.3–4.5 × 18.5–38.1 μm (n=100) in size; microconidia were absent; chlamydospores were produced in chains or clumps, ellipsoidal to subglobose, and thick walled. The morphological characteristics of the conidia were consistent with those of Fusarium spp. To identify the fungus, an EasyPure Genomic DNA Kit (TransGEN, Beijing, China) was used to extract the total genomic DNA from mycelia. The internal transcribed spacer region (ITS rDNA) and the translation elongation factor 1-α gene (TEF1) were amplified with primers ITS1/ITS4 (White et al. 1990) and EF1/EF2 (O’Donnell et al. 1998), respectively. Based on BLAST analysis, sequences of ITS (MT928727) and TEF1 (MT952337) showed 99.64% and 100% similarity to the ITS (MT939248.1), TEF1 (GQ505636.1) of F. ipomoeae isolates. Sequence analysis confirmed that the fungus isolated from the infected peanut was F. ipomoeae (Xia et al. 2019). The pathogenicity of the fungus was tested in the greenhouse. Twenty two-week-old peanut seedlings (cv. Huayu20) grown in 20-cm pots (containing autoclaved soil) were sprayed with a conidial suspension (105 ml−1) from a 15-day-old culture. Control plants were sprayed with distilled water. The experiment was conducted as a randomized complete block design, and placed at 25 °C under a 12-h photoperiod with 90% humidity. Symptoms similar to those in the field were observed on leaves treated with the conidial suspension ten days after inoculation, but not on control plants. F. ipomoeae was re-isolated from symptomatic leaves but not from the control plants. Reisolation of F. ipomoeae from inoculated plants fulfilled Koch's postulates. To our knowledge, this is the first report of F. ipomoeae causing peanut leaf spot in China. Our report indicates the potential spread of this pathogen in China and a systematic survey is required to develop effective disease management strategies.

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Regulation of flowering in acid lime (Citrus aurantifolia Swingle)

Indian Journal of Agricultural Research ◽

10.18805/ijare.v51i04.8428 ◽

2017 ◽

Vol 51 (04) ◽

Author(s):

M. Prabhu ◽

S. Parthiban ◽

A. Ramesh Kumar ◽

B. Usha Rani ◽

A. Jayasamundeeswari

Keyword(s):

Ascorbic Acid ◽

Salicylic Acid ◽

Plant Growth ◽

Vitamin C ◽

Block Design ◽

Ascorbic Acid Content ◽

Randomized Block Design ◽

The World ◽

Acid Lime ◽

Juice Content

India is the largest producer of acid lime in the world and they are used as fresh fruit or for the preparation of pickles and beverages. They are rich in vitamin C, minerals and salts. The seasonality of production leads to market glut which results in poor returns to the farmers. Hence, an experiment was conducted to manipulate and regulate flowering by using various plant growth regulators viz., GA3 and cycocel, chemicals viz., KNO3 and salicylic acid at 11 different treatment combinations. The experiment was conducted in randomized block design (RBD) and each treatment was replicated thrice. The age of the trees was seven years and the variety used was PKM1. The experiment conducted in four consecutive years. The experimental results showed that there were significant differences among the treatments. Acid lime trees sprayed with GA3 50 ppm in June + cycocel 1000 ppm in September + KNO3 2% in October recorded the highest number of fruits tree-1 (1003), weight of fruits (48.60 g) and average fruit yield (28.96 kg tree-1). The same treatment registered the highest values for juice content (32.13 %), TSS (7.29o Brix) and ascorbic acid content (32.56 mg / 100ml).

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Genetic control of resistance to gray leaf spot of maize in tropical germplasm

Cropp Breeding and Applied Biotechnology ◽

10.1590/s1984-70332012000200008 ◽

2012 ◽

Vol 12 (2) ◽

pp. 145-150 ◽

Cited By ~ 2

Author(s):

André Humberto de Brito ◽

Livia Maria Chamma Davide ◽

Renzo Garcia Von Pinho ◽

Rafael Pelloso de Carvalho ◽

Matheus Costa dos Reis

Keyword(s):

Genetic Control ◽

Leaf Spot ◽

Genetic Model ◽

Block Design ◽

Gray Leaf Spot ◽

Cercospora Leaf Spot ◽

Gene Effects ◽

Randomized Block Design ◽

Tropical Germplasm ◽

Plant Level

The main goal of this study was to assess the nature and magnitude of gene effects for resistance to Cercospora leaf spot. A randomized block design with three replications was used. The data were obtained at the plant level by assessing the disease severity. The data were analyzed per experiment, using the average data per plot. A dominant-additive genetic model without epistasis was considered, with estimation of the components of means and variance. The genetic control of resistance to gray leaf spot is polygenic with predominance of the additive effects. Dominance was observed in a few small-effect loci and high heritability values.

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Reaction of melon accessions to crater rot and resistance inheritance

Horticultura Brasileira ◽

10.1590/s0102-05362012000300017 ◽

2012 ◽

Vol 30 (3) ◽

pp. 459-465 ◽

Cited By ~ 3

Author(s):

Ítala JB Nascimento ◽

Glauber HS Nunes ◽

Rui Sales Júnior ◽

Katchen JP Silva ◽

Izabel M Guimarães ◽

...

Keyword(s):

Block Design ◽

Northeastern Brazil ◽

Distilled Water ◽

Conidial Suspension ◽

Randomized Block Design ◽

Injured Area ◽

Complex Inheritance ◽

Polyethylene Bags ◽

Myrothecium Roridum ◽

Susceptibility And Resistance

The aims of the present study were to assess the reaction of melon accessions to crater rot, caused by Myrothecium roridum, and study the inheritance of resistance. In the first experiment, 30 melon accessions from northeastern Brazil were assessed in a randomized block design with two replicates. Each plot was formed by a row of 12 plants, from which three fruits were sampled and used for inoculation. Each fruit was inoculated at four equidistant sites on the surface. Ten wounds were made at each site to an approximate depth of 3.0 mm, to which 0.05 mL of conidial suspension was placed at a concentration of 10(6) spores mL-1 of sterilized distilled water with the aid of a pipette. The fruits were placed on Petri dishes and enveloped in polyethylene bags containing hydrophilic cotton, both of which were moistened with sterilized distilled water. Five days after inoculation, the injured area (in mm²) was assessed. Accessions A-9, A-14 and A-30 were resistant to the CMM-609 isolate of M. roridum. In the second experiment, the parents AF-682 and UFERSA-05 were used as standards of susceptibility and resistance, respectively. From these plants, generations F1, F2, RC11 and RC12 were obtained. The six populations were grown under field conditions in a randomized block design. Resistance to crater rot on the melon fruits exhibited complex inheritance, with six loci and the involvement of additive and dominance effects.

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KAJIAN MACAM PUPUK HAYATI TERHADAP INTENSITAS PENYAKIT BERCAK DAUN Cercospora sp PADA TANAMAN JAGUNG HITAM

Innofarm:Jurnal Inovasi Pertanian ◽

10.33061/innofarm.v22i1.3534 ◽

2020 ◽

Vol 22 (1) ◽

pp. 50

Author(s):

Nining Saputri ◽

Sartono Joko Santosa ◽

Saiful Bahri

Keyword(s):

Leaf Spot ◽

Block Design ◽

Leaf Spot Disease ◽

Spot Disease ◽

Corn Husk ◽

Central Java ◽

Leaf Spots ◽

Randomized Block Design ◽

Disease Attack ◽

Biological Fertilizer

This study is titled Study of Biological Fertilizers on the intensity of Leaf Spots (Cercospora sp.) On Black Corn (Black Aztec) with the aim to study the types of biological fertilizers on the intensity of Cercospora sp. which began on November 9, 2019, in Jembangan Village, Gagaksipat Village, Ngemplak District, Boyolali District, Central Java, with a height of 150m (asl). This study uses a single factor Complete Randomized Block Design (RCBD) consisting of 10 treatments and 3 replications. The data from the results of this study were analyzed with the Duncan at the 5% level. The parameters observed include: intensity of leaf spot disease, weight of cob with corn husk per plant (gram), weight of cob without corn husk per plant (gram), weight of 100 corn seeds (gram). The results showed that: (1) Symptoms of leaf spot disease appear evenly on all biological fertilizers, symptoms of disease attack began to appear at the age of 30 days after planting. (2) In the treatment of biological fertilizer Megharizo on black corn concertration 10 ml/1,2 l (B2) can reduce the intensity of leaf spot disease. (3) The application of biological fertilizer from three doses has not been able to increase the yield of black corn to the weight of corn with corn husk, weight of corn without corn husk, and weight of 100 seeds.

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Mengembangkan skenario panen porang satu musim melalui manipulasi tanam lebih awal dan perlambatan waktu dorman fase generatif dengan pemberian asam salisilat organik alami di lahan kering Lombok Utara

Agromix ◽

10.35891/agx.v12i2.2596 ◽

2021 ◽

Vol 12 (2) ◽

pp. 74-78

Author(s):

Suparman ◽

Suwardji ◽

Kusnarta ◽

Sukartno

Keyword(s):

Salicylic Acid ◽

Block Design ◽

Slowing Down ◽

Randomized Block Design ◽

Randomized Design ◽

Breaking Dormancy ◽

Cultivation Technology ◽

Breaking Seed Dormancy ◽

Generative Phase ◽

Control Application

The research on the scenario of one-season porang harvest through crop manipulation and delaying dormancy time aims to determine the effect of salicylic acid in breaking the dormancy of porang bulbil seeds during seeding and to determine the effect of slowing down the dormant period of the generative phase, so it is expected to be an alternative porang cultivation technology that is profitable for farmers The method used in this study used a completely randomized design experiment on breaking dormancy and a randomized block design in an experiment with slowing down the dormancy time of the generative phase with four salicylic acid concentrations (0%, 0.5%, 1%, and 2%) with eight replicates were planted under rainfed conditions. The results showed that the concentration of salicylic acid (C7H6O3) 2% was the best treatment in increasing tuber weight (52.25%) compared to the control. Application of Salicylic Acid (2%) was significantly able to increase the vegetative growth of porang plants, which was shown by increasing plant height by 50.38% and breaking seed dormancy 14 days faster than the control (without the addition of Salicylic Acid). Furthermore, the delay in dormancy time occurred 1 month later than the control so that it could prolong the vegetative phase. The concentration of 2% salicylic acid is the best treatment, which can be recommended in the development of one-season porang cultivation technology that can increase farmers' profits.

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EFFECT OF SALICYLIC AND HUMIC ACID ON VINCRISTINE AND N, P, K CONTENT IN MADAGASCAR PERIWINKLE LEAVES

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v47i2.600 ◽

2016 ◽

Vol 47 (2) ◽

Author(s):

Jaddo & Rabee

Keyword(s):

Salicylic Acid ◽

Humic Acid ◽

Catharanthus Roseus ◽

Foliar Application ◽

Block Design ◽

Experimental Unit ◽

Madagascar Periwinkle ◽

Randomized Block Design ◽

Two Factors ◽

Four Levels

An Experiment carried out in Al-Yarmook Area in Baghdad for the growing season 2014 to study the effects of foliar application of Salicylic acid and Humic acid on Vincristine and NPK content in Catharanthus roseus. A factorial experiment was applied including two factors according to The Complete Randomized Block Design, the first factor included four levels of salicylic acid 0, 25, 50, and 100 mg.L-1 which were represented with S0, S1, S2, and S3 sequentially, the second factor included three levels of Humic acid 0, 0.75, and 1.5 ml.L-1 and were represented with O0, O1, and O2 sequentially to make up 12 factors in total that were distributed randomly upon three replicates and each experimental unit included 8 plants. The results were analyzed using Genestat and the differences were tested according to (LSD) with a level of probability 5% and the results were as follows: Vincristine levels increased in the leaves of Catharanthus roseus as a result of foliar application of Salicylic acid and Humic as well NPK levels in leaves. Vinblastine was not detected in all treatments. The interaction effect between 100 mg.L-1 Salicylic acid and 1.5 ml.L-1Humic acid had an exceeding effect for all the studied parameters. It may be concluded that Salicylic and Humic acid may be used to increase Vincristine alkaloid content in Periwinkle plants at the levels 100 ml.L-1 and 1.5 mg.L-1 subsequently.

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