scholarly journals Industrial and culinary practice effects on biologically active polyamines level in turkey meat

2021 ◽  
Vol 13 (2) ◽  
pp. 67-78
Author(s):  
Moein Bashiry ◽  
Hedayat Hoseini ◽  
Abdoreza Mohammadi ◽  
Ehsan Sadeghi ◽  
Nader Karimian-Khosroshahi ◽  
...  

Polyamines, including putrescine, spermidine, and spermine, are biological compounds present in nearly all food items. Their desirable physiological effects include cell division and growth. Hence, are undesirable in the diet of patients with tumor. This study aimed to assess the impact of curing agents (sodium chloride (0–2 g), sodium nitrite (0–200 ppm), sodium polyphosphate (0–0.5 g), and ascorbic acid (0–500 ppm)), cooking (frying (180°C), and boiling (100°C)) on polyamine contents in turkey breast meat using response surface methodology based on central composite design and dispersive liquid-liquid microextraction. Postprocessing changes were investigated using a high-performance liquid chromatography equipped with an ultraviolet detector. Study outcomes showed the presence of sodium chloride, sodium nitrite, and sodium polyphosphate in turkey meat reduced the putrescine and spermine content significantly (P < 0.0001). The addition of ascorbic acid as a curing agent slightly increased the concentration of polyamines, while no significant linear effects were associated with the thermal processes. The study observed that curing agents like sodium chloride, sodium nitrite, sodium polyphosphate, and ascorbic acid at 2 g, 200 ppm, 0.5 g, and 382 ppm, respectively, in frying mode minimized spermine and putrescine content with more than 96% desirability. In conclusion, curing additives and cooking are promising procedures for polyamine reduction in turkey breast meat.

2017 ◽  
Vol 737 ◽  
pp. 248-255 ◽  
Author(s):  
Tae Hee Kim ◽  
Dae Yeon Kim ◽  
Choong Sun Lim ◽  
Bong Kuk Seo

The preparation of high performance epoxy composites for industrial applications has been extensively researched. In this report, we study the change in physical properties and reaction kinetics between epoxy resin and curing agents of similar geometry. For the experiments, celloxide 2021P, an epoxy resin having low viscosity, was blended with three different curing agents: methylhexahydropthalic acid, methyltetrahydropthalic acid, and 5-norbornene-2, 3-dicarboxylic anhydride. The amount of 1, 2-dimethylimidazole catalyst was controlled, and the highest heat flow temperature (Tpeak) was observed at around 145 °C. The impact on reaction kinetics relative to the change in heating rate was studied with differential scanning calorimetry (DSC) for each of the curing agents. The glass transition temperature (Tg) of each composition was measured with a second DSC cycle. The prepared epoxy compositions were thermally cured in a metallic mold to provide pure epoxy resins without fillers. Finally, the flexural strengths of these resins were compared to each other. The authors believe that insights into choosing an appropriate epoxy binder are useful when it comes to the overall preparation of high performance polymer composites.


10.5219/1655 ◽  
2021 ◽  
Vol 15 ◽  
pp. 575-584
Author(s):  
Miroslava Kačániová ◽  
Simona Kunová ◽  
Peter Haščík ◽  
Karol Pietrzyk ◽  
Maciej Kluz ◽  
...  

The research was aimed to study the impact of sous vide thermal treatment on the microbiological quality of fresh turkey breast meat after treatment with thyme and rosemary EOs and the survival of Listeria monocytogenes on the turkey meat samples. The samples were vacuum-packed and cooked at 55 °C, 60 °C, and 65 °C for 5, 15, 30 and, 60 min. There was an amount of 5 g (5 ±0.2 g) of the sample placed in PA/PE film bags and inoculated with 100 μL of L. monocytogenes inoculum. The sample was incubated at 37 °C for 18 h after bag sealing. The samples were tested on the 1st and 3rd days of experiments. The microbiological quality of fresh turkey breast meat was assessed by the detection of total microbial counts and meat microbiota was identified by mass spectrometry using MALDI-TOF MS Biotyper (Bruker Daltonics, Germany). Microbial counts differed significantly depending on temperature and time and the microbial counts ranged from 2.21 log cfu.g-1 to 8.26 log cfu.g-1 on the 1st and 3rd day of the experiment. The study shows that the sous vide method with essential oils combination is an effective method and it can be used to protect the microbiota of turkey meat and L. monocytogens survival, however, the quality of raw material is crucial.


2021 ◽  
Vol 10 (3) ◽  
pp. 167-175
Author(s):  
D. A. Zhdanov ◽  
V. A. Kurkin ◽  
V. B. Braslavsky ◽  
A. I. Agapov

Introduction. Currently, herbal medicines are becoming increasingly popular. In this regard, of particular interest are medicinal herbal preparations based on rosehip fruits, which have a tonic, choleretic and wound healing effect due to the presence of ascorbic acid, flavonoids and carotenoids. According to the State Pharmacopoeia of the Russian Federation (SP RF) of the XIV edition, the determination of the main groups of biologically active substances (BAS) by thin-layer chromatography (TLC) is carried out only by the presence of ascorbic acid, while the sensitivity of the method does not allow it to be detected in rosehip fruits of low-vitamin species.Aim. Aim is the development of new approaches to control quality and standardization of Rosehip fruits with using of modern pharmacognostic analysis methods.Materials and methods. Prepared in Samara Region, the Republic of Mari El Rosehip fruits samples in 2020 and commercial samples from various manufacturers. TLC, UV/Vis-spectroscopy and high-performance liquid chromatography (HPLC) methods were used for determination the main BAS groups.Results and discussion. The introduction of flavonoids as one of the definitioned groups of BAS in rosehip fruits along with ascorbic acid was justified. A technique for the identification of ascorbic acid by UV/Vis-spectroscopy (wavelength 264 ± 2 nm) was developed and shown the determination possibility of this substance by HPLC.Conclusion. So phytochemical study results the expediency of the using of modern approaches to quality control and standardization of rosehip fruits, consisting in the qualitative determination of ascorbic acid by UV/Vis-spectroscopy and HPLC, as well as rutin by TLC, was justified.


2016 ◽  
Vol 24 (2) ◽  
Author(s):  
D. Serova ◽  
O. Taran ◽  
O. Dyomshina

One important reason for non-use of medicinal drugs is their hepatotoxicity. The search for new natural biologically active substances with hepatoprotective properties and which are cost-effective is an important task today. This article presents the data on the impact of the fodder-dietary supplement Humilid alone and in combination with ascorbic acid, and Eco-Impulse on the liver of Mongolian gerbils (Meriones unguiculatus Milne-Edwards, 1867). Hepatomodulation of properties of humic substances in the Humilid confirmed that stimulation of the activity of the enzyme transaminase increases the total amount of protein, especially in combination with ascorbic acid. There was inhibition of accumulation of oxidized products in the cell due to activation of catalase and superoxidedismutase. Intensification of catalase is regarded as one of the important mechanisms of adaptation of the cell under the influence of xenobiotics. Addition of ascorbic acid to the solution Humilid increased the antioxidant action of its active ingredients both through its own antioxidant properties and protection of humic acids from oxidation. Formation of response in liver cells of gerbils receiving active ingredients of the drug Eco-Impulse was accompanied by an increase in the number of TBA-active products and cytochrome C, which indicates the development of oxidative stress, which is accompanied by increased permeability of mitochondrial membranes. The results indicate the hepatoprotective properties of the active ingredients of the drug Humilid, alone and in combination with ascorbic acid, and the need for correcting the concentration of the drug Eco-Impulse for use in clinical practice. 


2020 ◽  
pp. 195-202
Author(s):  
Dmitriy Konstantinovich Gulyaev ◽  
Valentina Dmitriyevna Belonogova ◽  
Dmitriy Olegovich Bokov ◽  
Vladimir Vladimirovich Bessonov

The spruce Picea abies (L.) (Pinaceae) roots are logging residues and are not used nowadays. However, spruce roots are a source of valuable biologically active substances that have potential for medical use. The purpose is to research the composition of phenol compounds and antioxidative activity of spruce roots water extract. The raw materials for the research were the spruce roots collected on a cutting area on the day of sawing a tree. Water extract was obtained from the roots, and then polysaccharides were removed from it. Composition of the extract phenol compounds was determined using a high performance liquid chromatography on the chromatograph Agilent 1100. Compounds identification was based on retention times. Extract’s antioxidative activity was determined by the reaction with the stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH), the EC50 value computed. Ascorbic acid and trolox (a water-soluble form of vitamin E) were used as standard substances. As a result, it was found that flavonoids, hydroxycinnamic acids, phenolcarboxylic acids are present in the spruce roots water extract. Ferulic acid has the highest amount amongst hydroxycinnamic acids in the spruce roots water extract, hyperoside – amongst flavonoids. It was found that the spruce roots water extract, without polysaccharides, has a pronounced antioxidative activity similar in strength to ascorbic acid, which allows us to talk about the prospects for further researches and the medicinal drugs manufacturing.


1997 ◽  
Vol 77 (03) ◽  
pp. 504-509 ◽  
Author(s):  
Sarah L Booth ◽  
Jacqueline M Charnley ◽  
James A Sadowski ◽  
Edward Saltzman ◽  
Edwin G Bovill ◽  
...  

SummaryCase reports cited in Medline or Biological Abstracts (1966-1996) were reviewed to evaluate the impact of vitamin K1 dietary intake on the stability of anticoagulant control in patients using coumarin derivatives. Reported nutrient-drug interactions cannot always be explained by the vitamin K1 content of the food items. However, metabolic data indicate that a consistent dietary intake of vitamin K is important to attain a daily equilibrium in vitamin K status. We report a diet that provides a stable intake of vitamin K1, equivalent to the current U.S. Recommended Dietary Allowance, using food composition data derived from high-performance liquid chromatography. Inconsistencies in the published literature indicate that prospective clinical studies should be undertaken to clarify the putative dietary vitamin K1-coumarin interaction. The dietary guidelines reported here may be used in such studies.


2014 ◽  
Vol 1 (3) ◽  
pp. 3-7
Author(s):  
O. Zhukorskyy ◽  
O. Hulay

Aim. To estimate the impact of in vivo secretions of water plantain (Alisma plantago-aquatica) on the popula- tions of pathogenic bacteria Erysipelothrix rhusiopathiae. Methods. The plants were isolated from their natural conditions, the roots were washed from the substrate residues and cultivated in laboratory conditions for 10 days to heal the damage. Then the water was changed; seven days later the selected samples were sterilized using fi lters with 0.2 μm pore diameter. The dilution of water plantain root diffusates in the experimental samples was 1:10–1:10,000. The initial density of E. rhusiopathiae bacteria populations was the same for both experimental and control samples. The estimation of the results was conducted 48 hours later. Results. When the dilution of root diffusates was 1:10, the density of erysipelothrixes in the experimental samples was 11.26 times higher than that of the control, on average, the dilution of 1:100 − 6.16 times higher, 1:1000 – 3.22 times higher, 1:10,000 – 1.81 times higher, respectively. Conclusions. The plants of A. plantago-aquatica species are capable of affecting the populations of E. rhusiopathiae pathogenic bacteria via the secretion of biologically active substances into the environment. The consequences of this interaction are positive for the abovementioned bacteria, which is demon- strated by the increase in the density of their populations in the experiment compared to the control. The intensity of the stimulating effect on the populations of E. rhusiopathiae in the root diffusates of A. plantago-aquatica is re- ciprocally dependent on the degree of their dilution. The investigated impact of water plantain on erysipelothrixes should be related to the topical type of biocenotic connections, the formation of which between the test species in the ecosystems might promote maintaining the potential of natural focus of rabies. Keywords: Alisma plantago-aquatica, in vivo secretions, Erysipelothrix rhusiopathiae, population density, topical type of connections.


2016 ◽  
pp. 37-40
Author(s):  
S.I. Zhuk ◽  
◽  
K.K. Bondarenko ◽  

Most recent studies show the impact of violations in the metabolism of folate and metin period in the pathogenesis of neural tube defects (NTD) of the fetus. Metafolin has a number of advantages, which primarily includes direct intake of substances in biologically active form and the optimum effect, even in the case when the patient homozygote and/or heterozygote genotype 677С T polymorphism in MTHFR. With the aim of prevention and treatment of various pathological conditions related to folate deficiency during pregnancy, it is advisable to apply vitamin-mineral complexes, containing metafolin - active form of folate with high bioavailability. Key words: MTHFR, metafolin, folic acid, pregnancy.


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