Detection of Alpha-1 antitrypsin and Interleukin-8 in meningitis patients

Meningitis is a meninges inflammation and the subarachnoid cerebrum spinal fluid that surrounds the spinal cord and brain. Infantile meningitisis a major cause of morbidity & mortality in various parts of the world; it is earnest disorder owing to the nearness of the location to the brain andspinal cord. It can be caused by virtually any organism; in general viral infections are more common than bacterial, fungal and parasitic infectionsrespectively. Objective: investigation of the Interleukin-8 (IL-8) and Alaph-1 Antitrypsin in the cerebrum spinal fluid of meningitis patients.Material and Method: A study was done at the Medical city and Al-kadimia hospitals included 47 and 20 beings of Patients and Control groupswith an overall age range between 11 days to 6 years old. According to the causative agents of the disease, they were divided into three groups:bacterial, viral and control groups. All subjects were monitored for the IL-8 by Enzyme Linked Immunosorbent Assay (ELISA) and Alaph-1Anti-trypsin by Single Radial Immunodiffusion assay (SRIA) in the cerebrospinal fluid the data was analyzed via using SPSS (chi-squire test)program (version 22). Results and discussion: The results revealed the presence of an elevation concentration of IL-8 among meningitis diseasepatients (484.0695 pg/ml ), high concentration of IL-8 seemed in bacterial meningitis (825.3250pg/ml) when compared with its level in viralmeningitis (142.8143pg ml) ( p= 0.002) and control group ( 56.3409 pg/ml) (p=0.00) but no significant difference was found between viral andcontrol groups (p= 0.679). Alpha 1 antitrypsin results appeared high concentration of (21.6462 mg/ dl) and (16.4000 mg/ dl) in bacterial and viralmeningitis groups in sequentially, and that concentrations showed differences arrived significantly. The statistical analysis of this research showeda correlation between IL-8 levels and both of neutrophil and lymphocyte in meningitis patients. IL-8 had significant correlation with lymphocyte(p=0.02) in viral group while no significant correlation was appeared between IL-8 and neutrophil. In bacterial group, lymphocytes with IL-8 hadweak correlation, in contrary the correlation between neutrophil and IL-8 revealed the significantly correlation (p=0.01). No correlation was establish concerning the Alpha-1 antitrypsin and the cell’s numbers and types. Conclusion: A study indicated that Alpha 1 antitrypsin and IL-8 playedan important role in meningitis disease.

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P187 The significance of netrin-1 level in the clinical activity of ulcerative colitis, its association between TNF-α and IL-6

Journal of Crohn s and Colitis ◽

10.1093/ecco-jcc/jjz203.316 ◽

2020 ◽

Vol 14 (Supplement_1) ◽

pp. S232-S232

Author(s):

H Korkmaz ◽

K Fidan

Keyword(s):

Ulcerative Colitis ◽

Proinflammatory Cytokines ◽

Activity Index ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Clinical Activity ◽

Tnf Α ◽

Significant Difference ◽

And Control ◽

Control Study

Abstract Background In this study, we investigated the importance of netrin-1 levels in ulcerative colitis (UC) in clinical activity of the disease, and its association with other proinflammatory cytokines IL-6 and TNF-α. Methods This study is a type of case–control study. Sixty-seven patients with UC (36 of them activation, 31 of remission) and 50 healthy controls were included in the study. UC patients; ‘Truelove Witts clinical activity index by remission (n = 31), mild activation (n = 21), moderate activation (n = 6) and severe activation (n = 9) were divided into groups. Netrin, IL-6 and TNF-α measurements in plasma samples were performed using enzyme-linked immunosorbent assay kit. Results Between the patient group and the control group; there was a statistically significant difference between netrin-1, IL-6, TNF-α, neutrophil, platelet (p < 0.05 for all). The plasma netrin-1 mean of UC with severe activation group (139.21 ± 48.09 pg/ml) was statistically significantly higher than that of the mild activation (p = 0,037), remission group (p = 0,001) and control group(p = 0,011). The plasma netrin-1 mean of UC with moderate activation group was statistically significantly higher than that of the mild activation(p = 0,045) and remission group(p = 0,004). Conclusion Our results reveal that plasma netrin-1 levels have been shown to be associated with UC activation, similar to proinflammatory cytokines such as TNF-α and IL-6, in UC.

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Evaluation of Alpha-1 Antitrypsin Level in the Serum of Children With Idiopathic Bronchiectasis

ACTA MEDICA IRANICA ◽

10.18502/acta.v59i11.7775 ◽

2021 ◽

Author(s):

Maryam Babaei ◽

Zahra Kanannejad ◽

Soheila Alyasin

Keyword(s):

Serum Level ◽

Respiratory Infections ◽

Statistical Significance ◽

Clinical Syndrome ◽

Control Group ◽

Case Group ◽

Significant Difference ◽

Alpha 1 Antitrypsin ◽

One Year ◽

And Control

Bronchiectasis is a clinical syndrome characterized by chronic cough, sputum production, recurrent respiratory infections, and permanent bronchial dilation. The association between the level of alpha-1 antitrypsin (AAT) and bronchiectasis is controversial. In this study, we aimed to investigate this association in children with idiopathic bronchiectasis. The study was conducted on 20 patients with idiopathic bronchiectasis as the case group (mean age 15.9±2.1) and 20 healthy individuals as the control group (mean age 14.9±2.6). Serum AAT level was measured using nephelometric analysis (g/L). Other criteria including sex, parent consanguinity, number of hospitalizations, age of the first symptom were evaluated in both groups related to AAT level. The mean serum level of AAT in the case and control groups were 1.3±0.29; 1.5±0.59, respectively, with statistical significance (P=0.001). There was a significant difference between the two groups in the AAT level distribution, according to AAT normal range (P=0.01). The case group had a more positive attitude toward consanguinity than the control group (66.7% versus 33.3%; P<0.001). The results showed that 80% of patients had the first symptom of disease under one year of age, 6.6% 1-5 years, 6.6% 5-10 years, and 6.6% in more than ten years old. In the case group, 53.3% had a history of medical hospitalization for one time, 26.7% two times, while 20% of the patients had no medical hospitalization. Decreased AAT serum level and high consanguinity rates may be considered as two risk factors for idiopathic bronchiectasis occurrence in children.

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Important Role of B2-Glycoprotien in Females with Recurrence Abortion

International Journal of Pharmaceutical Quality Assurance ◽

10.25258/ijpqa.v9i01.11356 ◽

2018 ◽

Vol 9 (01) ◽

Author(s):

Ahmed Abbas Hasan ◽

Radhia Hussain Fadel ◽

Saeed Hilal Khudhair ◽

Doaa Hashim Jawad

Keyword(s):

Pearson Correlation ◽

Mean Value ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

P Value ◽

Recurrent Abortion ◽

Significant Difference ◽

Medical City ◽

And Gender ◽

Age Range

Recurrent abortion consider as one of the most common problem in world. There are different causes associated with recurrent abortion, these may be occur due to complication of microorganism infection or associated with immunological disturbance like abortion caused by Anti-phospholipid syndrome, or associated with SLE. The anti-b2glucoprotien antibodies have a role in abortion. In this study, we wish to determine whether there is a relationship between the levels of these anti-b2glucoprotien antibodies and abortion. Thirty females patients with spontaneous abortion (10 of them with Antiphospholipid syndrome,10 of them with systemic lupus erthymatosus and final 10 with toxoplasmosis with ages ranged between (20-46) where taken from (Al-Hussein Medical City/Kerbala). Control group consisted of 20 healthy people who were free from history of abortion who matched in age and gender with patients. B2gp(IgMandIgG) EASIA Kit, generic assay) and was studied using the enzyme-linked immunosorbent assay (ELISA) method. T-test and ANOVA and Pearson correlation used to analyze results by using SPSS version 24. P-value ≤ 0.05 was considered significant. The patients with age range blow 30 years show high percent(63.3%) among the other groups .most of the patients have high percent of 3 times abortion were reported in all different groups .The mean value of anti-B2gP-I IgG (45.354) show highly a significant difference results in aborted patients with APS and significant difference in patients with SLE when compared with controls groups. the Anti-B2gPI IgM not give statistically significant difference when camper to health cases. we conclude that the Anti-B2GPI IgG levels were increased significantly in aborted females with APS and SLE .

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Pengaruh Latihan Aerobik Ringan dan Sedang terhadap Kadar Interleukin 8 dan Jumlah Netrofil pada Remaja

Berkala Kedokteran ◽

10.20527/jbk.v12i1.357 ◽

2016 ◽

Vol 12 (1) ◽

pp. 61

Author(s):

Huldani Huldani

Keyword(s):

Blood Plasma ◽

Aerobic Exercise ◽

Plasma Levels ◽

Exercise Group ◽

Interleukin 8 ◽

Male Students ◽

Moderate Intensity ◽

Control Group ◽

Significant Difference ◽

And Control

Abstract: Aerobic exercise with mild to moderate intensity will improve health and fitness. Aerobic exercise stimulates an increase in the concentration of IL-8 and will attract neutrophils out of circulation and infiltrate into the damaged tissue for physical activity. To determine the effect of mild and moderate aerobic exercise on levels of interleukin 8 and the number of neutrophils in adolescents, semi-experimental research laboratory with the entire population of male students of SMAN I Banjarbaru. Determination of the sample with inclusion and exclusion criteria obtained 31 samples (9 mild aerobic exercise, moderate aerobic exercise 12 and 10 as a control). The implementation of data collection (blood plasma) immediately after the sample is finished doing aerobic exercise. Blood plasma samples were analyzed by flowcytometri to see the number of neutrophils and elisa to see the levels of Interleukin 8 (IL-8). The results showed that mean plasma levels of IL-8 in mild aerobic group (605.69 ± 123.28) and moderate (718.75 ± 132.55) is lower than the control group (720.80 ± 213.11). Kruskal Wallis statistic test in the three groups no significant difference with p = 0.320. The average number of neutrophils mild aerobic exercise group (52.42 ± 8.29) and moderate (63.60 ± 8.73) was higher than the control group (50.11 ± 5.55), which means that there is an increase in the number of neutrophils after aerobic exercise , with one-way ANOVA statistical test showed different significant with p = 0.001. LSD test found significant differences between mild and moderate aerobic group with p = 0.003, 95% CI. There is no difference between the control group mild aerobic with p = 0.519. There are differences between the groups of moderate aerobic and control with p = 0.000. It can be concluded that mild and moderate aerobic exercise did not affect the increased plasma levels of interleukin-8 in adolescents. Mild aerobic exercise had no effect on the increase in the number of neutrophils in adolescents. Moderate aerobic exercise influence on the increase in the number of neutrophils in adolescents.Keywords : Aerobic exercise, Interleukin 8, Neutrophyl Abstrak: Latihan aerobik dengan intensitas ringan sampai sedang akan meningkatkan kesehatan dan kebugaran tubuh. Latihan aerobik merangsang peningkatan konsentrasi IL-8 dan akan menarik netrofil dari sirkulasi dan menyusup kejaringan yang rusak karena aktivitas fisik. Untuk mengetahui pengaruh latihan aerobik ringan dan sedang terhadap kadar interleukin 8 dan jumlah netrofil pada remaja, dilakukan penelitian semi eksperimental laboratorik dengan populasi seluruh pelajar laki-laki SMAN I Banjarbaru. Penentuan besarnya sampel dengan kriteria inklusi dan ekslusi didapatkan 31 sampel (9 latihan aerobik ringan, 12 latihan aerobik sedang, dan 10 sebagai kontrol). Pelaksanaan pengambilan data (plasma darah) segera setelah sampel selesai melakukan latihan aerobik. Plasma darah sampel dianalisa dengan flowcytometri untuk melihat jumlah netrofil dan pemeriksaan elisa untuk melihat kadar Interleukin 8 (IL-8). Hasil penelitian menunjukkan bahwa rerata kadar IL-8 plasma pada kelompok aerobik ringan (605,69 ± 123,28) dan sedang (718,75 ± 132,55) lebih rendah dari kelompok kontrol (720,80 ± 213,11). Secara uji statistik Kruskal Wallis ketiga kelompok tidak ada perbedaan bermakna dengan nilai p = 0,320. Jumlah rerata netrofil kelompok latihan aerobik ringan (52,42 ± 8,29) dan sedang (63,60 ± 8,73) lebih tinggi daripada kelompok kontrol (50,11 ± 5,55), artinya ada peningkatan jumlah netrofil setelah latihan aerobik. Dengan uji statistik ANOVA satu arah didapatkan hasil yang bermakna berbeda dengan nilai p = 0.001. Uji LSD didapatkan perbedaan bermakna antara kelompok aerobik ringan dan sedang dengan nilai p = 0,003, IK 95 % . Tidak terdapat perbedaan antara kelompok aerobik ringan dengan kontrol dengan nilai p = 0,519. Terdapat perbedaan antara kelompok aerobik sedang dan kontrol dengan nilai p = 0,000. Dapat disimpulkan bahwa latihan aerobik ringan dan sedang tidak berpengaruh terhadap peningkatan kadar Interleukin-8 plasma pada remaja. Latihan aerobik ringan tidak berpengaruh terhadap peningkatan jumlah netrofil pada remaja. Latihan aerobik sedang berpengaruh terhadap peningkatan jumlah netrofil pada remaja. Kata Kata Kunci : Latihan Aerobik, Interleukin 8, Netrofil

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Tearfilm immunoglobulin E (IgE) level in vernal keratoconjunctivitis by ELISA

Kathmandu University Medical Journal ◽

10.3126/kumj.v7i2.2700 ◽

1970 ◽

Vol 7 (2) ◽

pp. 104-108 ◽

Cited By ~ 1

Author(s):

S Pokharel ◽

DN Shah ◽

SN Joshi ◽

M Choudhary

Keyword(s):

Hypersensitivity Reaction ◽

Immunoglobulin E ◽

Vernal Keratoconjunctivitis ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Female Group ◽

Type I ◽

High Concentration ◽

Type Iv ◽

Significant Difference

Background: Vernal keratoconjunctivitis (VKC) is recurrent chronic allergic conjunctivitis occurring in the prepubertal age-group with secondary involvement of the cornea and is self-limiting in character. The disease is prevalent worldwide but it shows predominance in the areas with dry and warm climate including the South Asia. VKC represents about 3% of the serious ophthalmic disease in some parts of the world where the prevalence is rate is high.Type I hypersensitivity reaction which is IgE-dependent and type IV hypersensitivity reaction have been implicated for the pathogenesis VKC. Objective: To determine level of immunoglobulin E (IgE) in the tear film of patients with Vernal Keratoconjunctivitis (VKC) attending outpatient department of BP Koirala Lions Centre for Ophthalmic Studies (BPKLCOS). Materials and methods: Thirty-four VKC patients and thirty-four controls were included in this study. Tear samples were collected using micro- capillary glass tube method and tear IgE levels were measured using an enzyme - linked immunosorbent assay (ELISA). Results: There was high concentration of tear IgE level in VKC (95.09IU/ml) than in controls (1.63IU/ml) though the difference was not statistically significant (p=0.16). No statistically significant difference was observed in male and female gender within VKC group and when compared with control group (in male group, p=0.21 and in female group, p=0.26). There was no statistically significant difference observed in tear IgE level in different age groups within VKC group and when compared with control group (p=0.30). The result did not show any significant difference in tear IgE level with respect to the duration of the disease (p=0.23).There was no statistically significant difference in tear IgE level with different episodes of VKC (p=0.69). No statistically significant difference of IgE concentration in tear was seen among different types of VKC (p=0.53) and grades of tarsal and limbal papillae (p= 0.72). Conclusion: There was high concentration of tear IgE level observed in VKC. Key words: Vernal keratoconjunctivitis; Tearfilm; IgE level; Type I hypersensitivity reaction; Type IV hypersensitivity reaction. DOI: 10.3126/kumj.v7i2.2700 Kathmandu University Medical Journal (2009) Vol.7, No.2 Issue 26, 104-108

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Association Between Cytomegalovirus Infection and Bad Obstetric Outcomes in Women from Kirkuk

International Journal of Public Health Science (IJPHS) ◽

10.11591/ijphs.v3i1.4672 ◽

2014 ◽

Vol 3 (1) ◽

pp. 29

Author(s):

Hala Mohamed Majeed Hassan ◽

Abdulghani Mohamed Alsamarai ◽

Zainab Khalil Aljumaili ◽

Amina Hamed Alobaidi ◽

Ferah Ghali Alsalihi

Keyword(s):

Viral Infections ◽

Obstetric Outcomes ◽

Control Group ◽

Serum Samples ◽

Previous Pregnancy ◽

Obstetric History ◽

Significant Difference ◽

Animal Exposure ◽

Age Range ◽

And Control

The human cytomegalovirus (CMV) is one of the common viral infections worldwide that represent a major causes of congenital infections. To determine the seroprevalence of CMV in women with bad obstetric history and sociodemographic characteristics that may influence the seropositivity, a case control descriptive prospective study was conducted in Kirkuk, Iraq. A 838 women with age range from 14 to 48 were included in the study. Of the total, 547 women were with bad obstetric history(BOH) and 291 women with normal previous pregnancy as control group. All the serum samples collected from the study and control groups were tested for CMV IgM and IgG antibodies by ELISA kits. CMV IgM seroprevalence was higher in women with BOH. CMV IgG seroprevalence was with no significant difference between BOH and control. CMV IgG seroprevalence significantly influenced by age, education, smoking, and family size. However, CMV IgM seroprevalence significantly associated with pregnancy, residence, and animal exposure. Odd ratio confirmed the association between CMV IgG and age, crowding index, residence, smoking, and number of abortion in women with BOH. In addition, current CMV infection significantly associated with residence in women with BOH.

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The concentration of testosterone, pituitary adenylate cyclase-activating polypeptide, and protamine 1 in the serum of male chicken following administration of epididymis and testicular extracts and their combination

Veterinary World ◽

10.14202/vetworld.2019.1101-1107 ◽

2019 ◽

Vol 12 (7) ◽

pp. 1101-1107 ◽

Cited By ~ 4

Author(s):

Gholib Gholib ◽

Rinidar Rinidar ◽

Fitriani Fitriani ◽

T. Zahrial Helmi ◽

Sugito Sugito ◽

...

Keyword(s):

Adenylate Cyclase ◽

Reproductive Organs ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Treatment Groups ◽

Significant Difference ◽

Pituitary Adenylate Cyclase ◽

Male Chicken ◽

And Control ◽

Protamine 1

Background and Aim: Testis and epididymis are male reproductive organs that play an important role in spermatogenesis. These two organs are rich in the content of hormones and other molecules needed in the process of spermatogenesis which affect the quality of the spermatozoa. The objective of this study was to examine the effect of the administration of epididymis and testicular extracts and their combination on testosterone, pituitary adenylate cyclase-activating polypeptide (PACAP), and protamine 1 (PRM1) concentrations in the serum of male chicken. Materials and Methods: Twenty male chickens (broiler strain Cp707), aged 3 weeks and weighing 800-1000 g, were randomly divided into four different groups including a control group (T0) = injected with 1 ml normal saline and treatment groups: T1 = injected with 1 ml epididymis extract, T2 = injected with 1 ml testicular extract, and T3 = injected with a combination of 1 ml epididymis + 1 ml testicular extract. The experiment was conducted for 13 days and at the end of the study (day 14), the chickens were sacrificed to obtain the serum. Furthermore, the concentrations of testosterone, PACAP, and PRM1 were then measured by using an enzyme-linked immunosorbent assay technique. Results: The concentrations of PACAP and PRM1 did not show a significant difference between treatment groups (T1, T2, and T3) and control group (T0) (p>0.05). However, the concentration of testosterone showed a significantly higher difference in a group injected with a combination of 1 ml epididymis and 1 ml testicular extracts (T3) compared to the control group (T0) (p<0.05). Conclusion: The administration of epididymis and testicular extracts and their combination did not affect the increase of PACAP and PRM1 concentration. However, a combination of these extracts significantly affects the increase of testosterone concentration in the serum of male chicken.

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Effect of Air Pollution on Salivary Interleukin-8 Levels in Children

World Journal of Dentistry ◽

10.5005/jp-journals-10015-1390 ◽

2016 ◽

Vol 7 (4) ◽

pp. 175-178

Author(s):

Solmaz Pourzare Mehrbani ◽

Zohreh Babaloo ◽

Zahra Jamali ◽

Tahmoores Abdollahian ◽

Hosein Eslami ◽

...

Keyword(s):

Air Pollution ◽

Interleukin 8 ◽

Assay Method ◽

Pollution Level ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

P Value ◽

Case Group ◽

Group I ◽

Significant Difference

ABSTRACT Background Air pollution in cities has always been a permanent and serious threat for the health of society and the environment, influencing the health of different body organs and systems both acutely and chronically. Therefore, the aim of this study is to investigate the effect of air pollution in saliva Interleukin-8 (IL-8) levels in children. Materials and methods A total of 88, 8- to 10-year-old children were included in this descriptive cross-sectional study. Group I, the case group, included 44 children from Tabriz (Iranian Azerbaijan), and group II, the control group, included 44 children from Kalibar Town (of Tabriz). Both groups in this study had a similar economic status. Following sampling and selection of individuals, for measuring the levels of saliva IL-8, ELISA (enzyme-linked immunosorbent assay) method was used. To investigate the difference between the means of the groups, independent t test or its nonparametric equivalent, i.e., Man—Whitney test and Statistical Package for the Social Sciences (SPSS) 21 were used. In this study, p-value lower than 0.05 was considered statistically significant. Results The results of this study indicate a significant difference in the levels of saliva IL-8 between the two groups, where the mean level of saliva IL-8 is greater in children residing in the region with higher air pollution level than in the children dwelling in the region with a greater air pollution level (p = 0.001). Conclusion The results obtained from this study indicated that the level of saliva IL-8 increases in regions with air pollution, potentially causing several mouth problems in children. How to cite this article Mehrbani SP, Babaloo Z, Jamali Z, Abdollahian T, Eslami H, Sobhani N. Effect of Air Pollution on Salivary Interleukin-8 Levels in Children. World J Dent 2016;7(4):175-178.

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Aqueous Levels of Pigment Epithelium-Derived Factor and Macular Choroidal Thickness in High Myopia

Journal of Ophthalmology ◽

10.1155/2015/731461 ◽

2015 ◽

Vol 2015 ◽

pp. 1-6 ◽

Cited By ~ 4

Author(s):

Wei Chen ◽

Yubo Guan ◽

Guanghui He ◽

Zhiwei Li ◽

Hui Song ◽

...

Keyword(s):

High Myopia ◽

Choroidal Thickness ◽

Pigment Epithelium ◽

Serum Levels ◽

Enhanced Depth Imaging ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Pigment Epithelium Derived Factor ◽

Significant Difference ◽

And Control

Purpose. To investigate the correlation between aqueous and serum levels of pigment epithelium-derived factor (PEDF) and macular choroidal thickness in high myopia patients, both with and without choroidal neovascularization (CNV).Methods. Serum and aqueous levels of PEDF were measured by enzyme-linked immunosorbent assay in 36 high myopia patients (36 eyes) with no CNV (non-CNV group), 14 high myopia patients (14 eyes) with CNV (CNV group), and 42 nonmyopia patients (42 eyes) (control group). Macular choroidal thickness was measured by enhanced-depth imaging optical coherence tomography.Results. Aqueous levels of PEDF were significantly higher in CNV group compared with non-CNV (P<0.001) and control (P<0.001) groups. Macular choroidal thicknesses were significantly decreased in the non-CNV and CNV groups compared with the control (P<0.001) group. A statistically significant difference (P=0.012) was found between the CNV and non-CNV groups. There was a positive correlation between aqueous PEDF and macular choroidal thickness in the non-CNV group (P=0.005), but no correlation with the CNV group. No correlation between serum PEDF and macular choroidal thickness was detected in the three groups.Conclusion. Variations in aqueous PEDF levels coincide with changes in macular choroidal thickness in high myopia patients with no CNV, while no such relationship exists in high myopia patients with CNV.

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