scholarly journals Molecular hydrogen as a possible therapeutic factor in complex rehabilitation therapy in patients with muscular skeletal disorders (literature review)

2021 ◽  
pp. 92-97
Author(s):  
Vasyl Makolinets ◽  
Tamara Grashenkova ◽  
Volodymyr Moseichuk ◽  
Kyrylo Makolinets ◽  
Vladyslav Moseichuk
Keyword(s):  
Free Radicals ◽  
Molecular Hydrogen ◽  
The Body ◽  
Matrix Proteins ◽  
Cartilage Tissue ◽  
Joint Diseases ◽  
Chain Reactions ◽  
Hydrogen Water ◽  
Normal Metabolism ◽  
The Brain

The paper presents an analysis of foreign scientific and medical data on the therapeutic factor — molecular hydrogen. The effectiveness of its application in the complex therapy of many diseases is revealed. The effect is achieved due to the small size of the mo­lecule, which passes through biological membranes and inhibits dangerous free radicals in the mitochondria, as well as in the nuc­leus, which reduces the possibility of DNA damaging. Molecular hydrogen neutralizes oxidants in the brain due to its ability to cross the blood-brain barrier. It normalizes the functions and metabolic processes in the body and, as an antioxidant, is selective: it does not affect the useful free radicals involved in important metabo­lic processes and selectively eliminates only the most dangerous oxidants — hydroxyl radicals. Interacting with them, hydrogen converts them into water molecules without the formation of by-products and chain reactions. Unlike other known antioxidants, molecular hydrogen does not disrupt normal metabolism, does not cause negative changes in cells, activates the body’s own anti­oxidant systems. The possibility and expediency of the use of molecular hydrogen in the case of pathology of the musculoskeletal system has been confirmed. The peculiarities of its effect on bone and cartilage tissue in the experiment are shown. It has been determined that the use of molecular hydrogen is a new pharmacological strategy aimed at the selective removal of ONOO—, and can be an effective method in the treatment of joint diseases. Because cartilage receives nutrients through a diffusion-loading mechanism, and molecular hydrogen penetrates rapidly into tissues, it can be useful for the prevention of diseases of joints of degenerative origin. It reduces oxidative stress and slows down the reduction of matrix proteins and inhibition of proteinase degradation. Its effectiveness has been proven after injuries to the spinal cord, muscles and tendons, comorbid diseases such as hypertension, coronary heart disease, diabetes and metabolic syndrome. Key words. Molecular hydrogen, hydrogen water, hydrogen inhalations, joint diseases, consequences of musculoskeletal injuries, comorbid pathology.

Investigating Benzene and Metabolite Mediated Alterations in Nrf2 Signaling in Murine Fetal Liver Cells

2018 ◽  
Author(s):  
Enoch Lam
Keyword(s):  
Free Radicals ◽  
Cell Cultures ◽  
Childhood Leukemia ◽  
Fetal Liver ◽  
White Blood Cells ◽  
Chemical Exposure ◽  
The Body ◽  
Chain Reactions ◽  
Additional Cell ◽  
Other Substances

Leukemia is a cancer of the blood, where the body produces an overabundance of immature white blood cells. It is the most common childhood cancer and possibly originates from chemical exposure during fetal development. Understanding the molecular mechanism and causes of childhood leukemia will help the development of therapeutic and preventative strategies to reduce and treat occurrences of this cancer. Benzene is a non-polar aromatic ring that has been confirmed to cause leukemia in adults. Benzene metabolites can generate free radicals that could potentially be involved in the development of childhood leukemia through in utero exposures. It is found in tobacco smoke, gasoline, industrial solvents and many other substances. Pregnant mothers can be chronically exposed to these substances through daily activities. In order to help determine the mechanism of benzene toxicity, a study was performed to determine the presence of free radicals in the livers of fetal mice at gestation day 14. Fetal liver cell cultures were exposed to varying concentrations of benzene and additional cell cultures were exposed to various concentrations of a benzene metabolite mixture, composed of hydroquinone and benzoquinone. RNA was extracted from these cells and converted into complementary DNA. cDNA was then used in quantitative polymerase chain reactions to analyze a set of genes that are regulated by an antioxidant pathway. This pathway can be activated in response to oxidative stress, and a change in gene expression would indicate the presence of benzene or benzene metabolite toxicity in the liver at gestation day 14.

scholarly journals Antioxidant Drug Design: Historical and Recent Developments

2021 ◽  
pp. 36-56
Author(s):  
Melford C. Egbujor ◽  
Samuel A. Egu ◽  
Vivian I. Okonkwo ◽  
Alifa D. Jacob ◽  
Pius I. Egwuatu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radicals ◽  
Drug Design ◽  
The Body ◽  
Antioxidant Defenses ◽  
Cell Aging ◽  
High Demand ◽  
Chain Reactions ◽  
Single Piece ◽  
Recent Developments

The sustained interest in the design of potent antioxidants drugs over the years can be attributed to the indispensable roles antioxidants play in the mitigation of oxidative stress and its concomitant diseases. The high demand for exogenous antioxidants has been ascribed to the prevalence of oxidative stress-mediated diseases such as cancer, diabetes, stroke, cell aging, arteriosclerosis and central nervous system disorders occasioned by a biochemical disequilibrium between the production of free radicals and the body’s ability to eliminate these reactive species from the biological system. COVID-19 severity and death have been linked to a free radical generating process known as the cytokine storm. In an attempt to maintain optimal body function, antioxidant supplementation has increasingly become a wide spread practice because of antioxidants’ ability to directly scavenge free radicals, inhibit oxidative chain reactions thereby increasing the antioxidant defenses of the body. Recent data showed that researchers had made significant efforts to demonstrate the importance and timeliness of antioxidant therapy based on drug design from natural and synthetic sources. Therefore this review presents antioxidant drug design methodologies, identifying the lead and hits to provide a historical and up-to-date collection of research briefs on antioxidant drug design into a single piece in order to ensure easy accessibility, motivate readership and inspire future researches.

Pengembangan Potensi Ekstrak Belut (Monopterus albus) dalam Menangkal Radikal Bebas

2021 ◽  
Vol 8 (1) ◽  
pp. 88
Author(s):  
Havizur Rahman ◽  
Putri Maya Sari ◽  
Fitrianingsih Fitrianingsih ◽  
Ai Kurniati ◽  
Fitri Kurniawati
Keyword(s):  
Free Radicals ◽  
Freeze Drying ◽  
Cell Damage ◽  
Radical Scavenging ◽  
The Body ◽  
Meat Sample ◽  
Strong Activity ◽  
Monopterus Albus ◽  
Chain Reactions ◽  
Albumin Content

Penelitian ini bertujuan untuk mengetahui aktifitas antioksidan dari ekstrak belut (Monopterus albus) yang hidup di perairan provinsi jambi. Radikal bebas cenderung menimbulkan reaksi berantai yang apabila terjadi di dalam tubuh akan dapat menimbulkan kerusakan sel yang berlanjut dan terus menerus terutama pada penyakit kronis seperti diabetes, hipertensi dan hiperkolesterol. Pencegahan dapat dilakukan dengan memberikan seyawa antioksidan, salah satunya asam amino. Di dalam al-Quran disebutkan bahwa bangkai yang halal untuk dimakan adalah ikan dan belalang. Belut merupakan salah satu jenis ikan yang memiliki kandungan albumin yang tinggi. Sampel daging belut yang telah difillet dan dipisahkan dari kepalanya, dipotong kecil-kecil dengan ukuran 1,5 cm2 dan dibalut dengan kain tipis dalam sebuah mangkuk, lalu dikukus, dipress, dan disentrifus.  diambil fase air dan minyak dan dibuang pengotornya dengan cara disaring, lalu dikeringkan menggunakan freeze drying sehingga diperoleh ekstrak belut dalam bentuk serbuk. Metode yang digunakan pada penelitian ini adalah Radical Scavenging method menggunakan senyawa kimia DPPH (2,2- difenil-1-pikrilhidrazil). Dari hasil penelitian ditemukan bahwa nilai IC50 ekstrak belut sebesar 29,0816 ppm. Ekstrak belut memiliki potensi menangkal radikal bebas dengan aktifitas kuat. Kata Kunci: Ektstrak Belut, Monopterus albus, Penangkal Radikal Bebas This study aims to determine the antioxidant activity of eel (Monopterus albus) extracts that live in the waters of the province of Jambi. Free radicals tend to cause chain reactions that occur in the body and will cause ongoing and continuous cell damage, especially in chronic diseases such as diabetes, hypertension and hypercholesterolemia. Prevention can be done by providing antioxidants, one of which is amino acids. In the Koran, it is stated that the carcasses that are lawful to eat are fish and grasshoppers. Eel is a type of fish that has a high albumin content. The eel meat sample that has been filled and given from its head, is cut into small pieces with a size of 1.5 cm2 and wrapped in a thin cloth in a container, then steamed, pressed, and centrifuged. the air and oil phases are taken and the impurities are removed by filtering, then they are dried using freeze drying in order to obtain the eel extract in powder form. The method used in this study is the Radical Scavenging Method using the chemical compound DPPH (2,2- diphenyl-1-pikrilhidrazil). From the research results it was found that the IC50 value of eel extract was 29.0816 ppm. Eel extract has the potential to ward off free radicals with strong activity.

scholarly journals Molecular Targets of Natural Products for Chondroprotection in Destructive Joint Diseases

2020 ◽  
Vol 21 (14) ◽  
pp. 4931
Author(s):  
Thanasekaran Jayakumar ◽  
Periyakali Saravana Bhavan ◽  
Joen-Rong Sheu
Keyword(s):  
Natural Products ◽  
Proteolytic Enzymes ◽  
Biological Activities ◽  
Biological Effects ◽  
The Body ◽  
Matrix Proteins ◽  
Joint Diseases ◽  
Therapeutic Drugs ◽  
Therapeutic Value ◽  
Long Time

Osteoarthritis (OA) is the most common type of arthritis that occurs in an aged population. It affects any joints in the body and degenerates the articular cartilage and the subchondral bone. Despite the pathophysiology of OA being different, cartilage resorption is still a symbol of osteoarthritis. Matrix metalloproteinases (MMPs) are important proteolytic enzymes that degrade extra-cellular matrix proteins (ECM) in the body. MMPs contribute to the turnover of cartilage and its break down; their levels have increased in the joint tissues of OA patients. Application of chondroprotective drugs neutralize the activities of MMPs. Natural products derived from herbs and plants developed as traditional medicine have been paid attention to, due to their potential biological effects. The therapeutic value of natural products in OA has increased in reputation due to their clinical impact and insignificant side effects. Several MMPs inhibitor have been used as therapeutic drugs, for a long time. Recently, different types of compounds were reviewed for their biological activities. In this review, we summarize numerous natural products for the development of MMPs inhibitors in arthritic diseases and describe the major signaling targets that were involved for the treatments of these destructive joint diseases.

scholarly journals STATE AND PROSPECTS USING MOLECULAR HYDROGEN FOR ATHLETES

2020 ◽  
Vol 1 ◽  
pp. 443-450
Author(s):  
Oleg Pokotylo ◽  
◽  
Ivan Zakharchuk ◽  
Borys Vykhovanets ◽  
◽  
...  
Keyword(s):  
Sports Medicine ◽  
Molecular Hydrogen ◽  
The Body ◽  
Alternative Mechanism ◽  
Physically Active ◽  
Hydrogen Water ◽  
Positive Effects ◽  
Advantages And Disadvantages ◽  
The Individual ◽  
Effective Use

Introduction. The study of molecular hydrogen as the latest therapeutic and prophylactic corrector of metabolism has been successfully tested on more than 170 models of pathological conditions. Its effective antioxidant, cytoprotective, anti-inflammatory effect on the body has been proven. Separate studies of the effects of molecular hydrogen have been conducted on athletes. The aim of the study - to investigate the level of research and efficiency of using molecular hydrogen in sports medicine and to predict the algorithm of its further research and practical use. Research Methods: Analysis, generalization of data of professional scientific literature for researching the effect of molecular hydrogen in physical culture and sports and identify opportunities and criteria for its use for athletes and physically active people Results. An analytical study based on the results of professional publications about the effective use of molecular hydrogen for athletes in the world was conducted. The advantages and disadvantages of using molecular hydrogen for the organism are reasoned. Ways and methods of entry of molecular hydrogen into the body are described. Inventions of molecular hydrogen generators of Ukrainian scientists, their advantages over foreign analogues and possibilities of their application in sports are shown. Conclusions. Today in view of the analysis of the situation with the use of molecular hydrogen for therapeutic and prophylactic purposes in medicine and sports medicine in particular, the algorithm of prospects for its further research and practical application follows. Firstly, to study the optimal protocol for the intake of molecular hydrogen, depending on the intensity of physical activity, body weight, age, sex, comorbidities, etc.; secondly, to develop personalized antioxidant strategies for the using molecular hydrogen, taking into account the individual redox characteristics of individuals; thirdly, to investigate the consequences of long-term intake of H2 during exercise; fourthly, to reveal the alternative mechanism underlying the antioxidant action and other positive effects of molecular hydrogen. Key words: sports medicine, hydrogen water, «TIG «Liwing Water»», redox potential, oxidative stress.

scholarly journals Drinking Molecular Hydrogen Water Is Beneficial to Cardiovascular Function in Diet-Induced Obesity Mice

Biology ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 364
Author(s):  
Haruchika Masuda ◽  
Atsuko Sato ◽  
Kumiko Miyata ◽  
Tomoko Shizuno ◽  
Akira Oyamada ◽  
...  
Keyword(s):  
Molecular Hydrogen ◽  
Left Ventricular ◽  
Heart Weight ◽  
Therapeutic Effects ◽  
Protective Effects ◽  
Radical Chain ◽  
Chain Reactions ◽  
Hydrogen Water ◽  
Diet Induced Obesity ◽  
Brown Adipose

Molecular hydrogen (MH) reportedly exerts therapeutic effects against inflammatory diseases as a suppressor of free radical chain reactions. Here, the cardiovascular protective effects of the intake of molecular hydrogen water (MHW) were investigated using high-fat diet-induced obesity (DIO) mice. MHW was prepared using supplier sticks and degassed water as control. MHW intake for 2 weeks did not improve blood sugar or body weight but decreased heart weight in DIO mice. Moreover, MHW intake improved cardiac hypertrophy, shortened the width of cardiomyocytes, dilated the capillaries and arterioles, activated myocardial eNOS-Ser-1177 phosphorylation, and restored left ventricular function in DIO mice. MHW intake promoted the histological conversion of hypertrophy to hyperplasia in white and brown adipose tissues (WAT and BAT) with the upregulation of thermogenic and cardiovascular protective genes in BAT (i.e., Ucp-1, Vegf-a, and eNos). Furthermore, the results of a colony formation assay of bone-marrow-derived endothelial progenitor cells (EPCs) indicated that MHW activated the expansion, differentiation, and mobilization of EPCs to maintain vascular homeostasis. These findings indicate that the intake of MHW exerts cardiovascular protective effects in DIO mice. Hence, drinking MHW is a potential prophylactic strategy against cardiovascular disorders in metabolic syndrome.

Anti-Aging Effects of Deoxyschizandrin in D-Galactose-Induced Aging Rats

2013 ◽  
Vol 423-426 ◽  
pp. 373-377 ◽  
Author(s):  
Xiao Dong Huang ◽  
Jian Ying ◽  
Yan Chun Wang ◽  
Kuang Ren
Keyword(s):  
Free Radicals ◽  
Water Maze ◽  
The Body ◽  
High Dose ◽  
Aging Effects ◽  
Moderate Dose ◽  
Aging Rats ◽  
Inhibit Lipid Peroxidation ◽  
The Brain ◽  
Brain Tissues

Objective: To investigate anti-aging effects of deoxyschizandrin in rats and its mechanisms. Methods: D-galactose (120mg • kg-1 • d-1) was subcutaneously injected daily for 6 weeks to build a rat aging model, and deoxyschizandrin (50,100 and 200 mg • kg-1 • d-1) was consecutively administered daily for six weeks from the second day. Morris water maze was used for the observation of learning and memorizing abilities of the rats; spectrophotometry was applied to detect malondialdehyde (MDA) contents, superoxide dismutase (SOD) activities, glutathione peroxidase (GSH-Px) activities, Na+-K+-ATPase and Ca2+-ATPase activities in the rats’ brain tissues. Results: The results showed that moderate-dose and high-dose deoxyschizandrin could improve learning and memorizing abilities of D-galactose-induced aging rats, enhance SOD, GSH-Px, Na+-K+- and Ca2+-ATPase activities, and reduce MDA levels in the rats’ brain tissues significantly (P <0.05 or P <0.01). Conclusion: deoxyschizandrin can improve rats’ learning and memorizing abilities, and its mechanisms may be associated with the increase in antioxidant enzymes such as SOD and GSH-Px in the body, the decrease in the production of MDA, the enhancement of ability to scavenge free radicals and inhibit lipid peroxidation to protect the brain cells from the damage by free radicals.

scholarly journals MOLECULAR HYDROGEN GENERATOR GVCH LIFE

2021 ◽  
pp. 65-68
Author(s):  
Volodymyr Moseichuk ◽  
Vladyslav Moseichuk ◽  
Vasyl Makolinets
Keyword(s):  
Musculoskeletal System ◽  
Molecular Hydrogen ◽  
Water Concentration ◽  
Hydrogen Gas ◽  
The Body ◽  
Water Volume ◽  
Antioxidant Systems ◽  
Pure Hydrogen ◽  
Hydrogen Generator ◽  
Hydrogen Water

Molecular hydrogen is one of the effective antioxidants, which not only does not disrupt normal metabolism in the body, but also activates its antioxidant systems. Hydrogen-saturated water has  antioxidant, anti-inflammatory, anti-allergic, anti-apoptotic properties, stimulates energy metabolism and contributes to the systemic recovery of the body. It is used as a therapeutic factor for the treatment of patients with various pathologies: arterial hypertension, coronary heart disease, diabetes, obesity, metabolic disorders, disorders of the musculoskeletal system. The article discusses the various methods of obtaining molecular hydrogen and hydrogen water (direct and indirect saturation). Technical characteristics are described and features of the hydrogen generator GVCh Life (manufacturer LTD «Chemtest Ukraine+», Kharkiv, Ukraine), which produces molecular hydrogen (purity of which is 99.99 %, productivity — 100 ml/min) and saturates water with it (https://chemtest.com.ua/generator_vodorodnoy_vodi_i_dihanie_gvch_life). In contrast to the problems of most known generators in the device GVChLife is completely no contact of the electrodes with water, so it is not subject to electrolysis and is not saturated with metal ions. Water saturated in this way has the following characteristics: redox potential 560 mV, hydrogen concentration 1.0–1.15 ppm(water volume 1 l, saturation duration 10 min). The generator can be used for both hydrogen saturation and hydrogen inhalation. In the case of therapeutic use of hydrogen water to obtain it, you can use any drinking water (spring, prepared or non-carbonated bottled), hydrogen inhalation using nasal cannulas. Inhalation of pure hydrogen gas (99.99 %) for 30 minutes is equal to the use of 15 liters of hydrogen water (concentration 1.1–1.2 ppm). Conclusions. The developed MoHC Life molecular hydrogen generator is safe to use, without special requirements during operation. It can be successfully used in the complex therapy of patients with various diseases, including musculoskeletal system.

EXPERIMENTAL ASSESSMENT OF THE NANOPARTICLES TOXICITY OF NICKEL OXIDE IN TWO SIZES IN THE SUBCHRONIC EXPERIMENT

2018 ◽  
pp. 30-35
Author(s):  
M.P. Sutunkova ◽  
B.A. Katsnelson ◽  
L.I. Privalova ◽  
S.N. Solovjeva ◽  
V.B. Gurvich ◽  
...  
Keyword(s):  
Nickel Oxide ◽  
Nervous Activity ◽  
Equal Mass ◽  
The Body ◽  
Subchronic Toxicity ◽  
Physiological Mechanisms ◽  
Nickel Oxide Nanoparticles ◽  
The Relationship ◽  
The Brain ◽  
Nanoparticles Toxicity

We conducted a comparative assessment of the nickel oxide nanoparticles toxicity (NiO) of two sizes (11 and 25 nm) according to a number of indicators of the body state after repeated intraperitoneal injections of these particles suspensions. At equal mass doses, NiO nanoparticles have been found to cause various manifestations of systemic subchronic toxicity with a particularly pronounced effect on liver, kidney function, the body’s antioxidant system, lipid metabolism, white and red blood, redox metabolism, spleen damage, and some disorders of nervous activity allegedly related to the possibility of nickel penetration into the brain from the blood. The relationship between the diameter and toxicity of particles is ambiguous, which may be due to differences in toxicokinetics, which is controlled by both physiological mechanisms and direct penetration of nanoparticles through biological barriers and, finally, unequal solubility.

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