scholarly journals Fluoride Concentration in SDF Commercial Products and Their Bioavailability with Demineralized Dentine

2020 ◽  
Vol 31 (3) ◽  
pp. 257-263
Author(s):  
Aline Laignier Soares-Yoshikawa ◽  
Jaime Aparecido Cury ◽  
Cínthia Pereira Machado Tabchoury
Keyword(s):  
Fluoride Concentration ◽  
In Vitro Study ◽  
Test Strip ◽  
T Test ◽  
The Other ◽  
Alkaline Ph ◽  
Commercial Products ◽  
Silver Diamine Fluoride ◽  
Direct Technique

Abstract The aim of this in vitro study was to determine the fluoride concentration in silver diamine fluoride (SDF) products and their bioavailability with demineralized dentine. The products evaluated (expected fluoride concentrations) were: I: Saforide 38% (45,283 ppm F); II: Advantage Arrest 38.3 to 43.2% (45,283 to 51,013 ppm F); III: Ancárie 12% (14,100 ppm F); IV: Ancárie 30% (35,400 ppm F), V: Cariestop 12% (14,100 ppm F) and VI: Cariestop 30% (35,400 ppm F). The fluoride concentration was evaluated using an ion-specific electrode (ISE) by direct technique, which was confirmed after microdiffusion. The pH of the products was determined with a pH test strip. For the bioavailability test, demineralized dentine slabs were treated with one of the products for 1 min. Loosely (CaF2-like) and firmly-bound fluoride (FAp) were determined. The fluoride concentration found in the products (mean±SD; ppm F) by the ISE direct technique was: I:53,491±554; II:57,249±1,851; III:4,814±268; IV:5,726±43; V:10,145±468; VI:11,858±575; these values were confirmed after microdiffusion (t-test; p>0.05) and disagree with the declared by the manufacturers. The pH of Ancárie 12 and 30% was 6.0 and 4.5, respectively, in disagreement with the alkaline pH expected for SDF solution and found in the other products evaluated. There was no correlation between either CaF2-like (r=0.221; p=0.337) or FAp (r=-0.144; p=0.830) formed in demineralized dentine and fluoride concentration found in the products. The problems of pH and fluoride concentration found in available professional commercial SDF products suggest that they are not under sanitary surveillance.

scholarly journals An in-vitro study to evaluate the accuracy of dies obtained from dual arch impression trays using addition silicone impression material and tray combinations

2015 ◽  
Vol 12 (2) ◽  
pp. 111-117
Author(s):  
P K Parajuli ◽  
S George ◽  
V Shenoy
Keyword(s):  
In Vitro Study ◽  
Dimensional Accuracy ◽  
Acrylic Resin ◽  
Simultaneous Recording ◽  
T Test ◽  
Impression Material ◽  
Tooth Preparation ◽  
Made In ◽  
Plastic Trays

Background: Dual-arch impression technique allows the simultaneous recording of  tooth preparation, opposing anatomic tooth and maxillomandibular relationship. The  accuracy of reproduction of this easy and quick technique, however, has not been  studied in detail in past. Objective: To compare the accuracy of the impressions  made by using the same impression material in dual arch plastic trays, dual-arch  metal trays and acrylic resin custom trays. Methods: The dies obtained from the  addition silicone impressions made in dual-arch plastic trays, dual-arch metal trays  and full arch acrylic resin custom trays were compared for the dimensional accuracy  with the prepared typodont tooth as a control. Student’s paired t-test and unpaired  t-test were used for the data analyses using the Statistical Package for Social Studies  (SPSS) version 11.5. Results: The dies obtained from all the impression combinations  showed increased dimension (acrylic resin custom trays 9.4 mm±0.048, dual-arch  plastic trays 9.5 mm±0.035, dual-arch metal trays 9.41 mm±0.017) as compared to  the dimension of control (9.39 mm±0.007). Conclusion: All the tray-impression  material combinations showed variable accuracies. Full arch acrylic resin trays  resulted in greatest accuracy whereas dual-arch plastic trays the least accuracy.Health Renaissance 2014;12(2):  pp: 111-117

scholarly journals Intratubular Enterococcus faecalis viability assessment following root canal instrumentation with rotary and reciprocating systems via fluorescence microscopy

2020 ◽  
Vol 14 (4) ◽  
pp. 214-217
Author(s):  
Jamileh Ghoddusi ◽  
Ehsan Arian ◽  
Maryam Golmohammadi ◽  
Maryam Gharechahi ◽  
Siavash Moushekhian
Keyword(s):  
Enterococcus Faecalis ◽  
In Vitro Study ◽  
Sampling Procedure ◽  
T Test ◽  
Root Canals ◽  
Paired Samples ◽  
Viable Bacteria ◽  
Root Canal Instrumentation ◽  
Reciprocating Systems

Background. The present in vitro study aimed to compare the effectiveness of the WaveOne and ProTaper Gold systems in removing the Enterococcus faecalis biofilm. Methods. Thirty-eight mandibular premolars were selected. The root canals were assigned to standard control (canals serially enlarged with ProTaper Gold S1-S2-F1-F2, n=15) and experimental (canals enlarged with Primary WaveOne file, n=15) groups. Following the instrumentation procedure, the root canals underwent a sampling procedure, and the colonyforming unit (CFU) counts were determined. The samples were also evaluated under a fluorescent microscope to evaluate viable bacteria. The data were analyzed using independent samples t test and paired samples t test. Results. The results showed that, compared with the ProTaper group, the WaveOne group exhibited the least viable bacteria (P=0.004). Conclusion. It was concluded that comparison with the ProTaper Gold rotary system, the WaveOne reciprocating file is more successful in reducing intratubular viable bacteria counts.

An In-Vitro Investigation into the Cement Pressurization Achieved During Insertion of Four Different Femoral Stems

2005 ◽  
Vol 219 (6) ◽  
pp. 407-413 ◽  
Author(s):  
C Gozzard ◽  
S Gheduzzi ◽  
A W Miles ◽  
I D Learmonth
Keyword(s):  
Revision Surgery ◽  
In Vitro Study ◽  
The Other ◽  
Stem Volume ◽  
Factors Associated ◽  
In Vitro Investigation ◽  
Femoral Stems ◽  
Stem Shape ◽  
Distal Stem

Adequate cement pressurization during stem insertion improves the interdigitation of cement into bone. This increases the strength of the cement-bone interface, thus contributing to the reduction of the incidence of aseptic loosening, the commonest cause of revision surgery. This in-vitro study compared the cement pressurization achieved during insertion of four different stems of equivalent sizes: the Elite Plus (DePuy, UK), C-Stem (DePuy, UK), Exeter (Stryker, USA), and CPS-Plus (Plus Orthopedics, Switzerland). The maximum pressures attained at the time of stem insertion were recorded at proximal, mid and distal stem levels. The Elite Plus generated significantly higher distal pressures than the other stems. The CPS-Plus generated significantly greater proximal cement pressures than the Elite Plus, C-Stem, and Exeter prostheses. The triple taper of the C-Stem increased the cement pressurization medial to the stem. The stem shape and the presence or absence of a proximal stem centralizer affect cement pressurization. The presence of a proximal stem centralizer, a large stem volume, and a lateral-medial taper are all factors associated with increased cement pressurization during stem insertion.

scholarly journals A COMPARATIVE EVALUATION OF MICROLEAKAGE, COMPRESSIVE STRENGTH, FLEXURAL STRENGTH AND FLUORIDE RELEASE OF ZIRCONOMER AND KETAC SILVER: AN IN VITRO STUDY.

2020 ◽  
Vol 9 (4) ◽  
Author(s):  
CHARANTEJA VEMAGIRI ◽  
Uloopi KS ◽  
Vinay Chandrappa ◽  
Anusha Ch
Keyword(s):  
Compressive Strength ◽  
Flexural Strength ◽  
Testing Machine ◽  
In Vitro Study ◽  
Biological Properties ◽  
T Test ◽  
Fluoride Release ◽  
Restorative Material ◽  
Glass Ionomer

Background: A successful restorative material forms a better adhesion, resist the microleakage and releases fluoride. However, existing glass ionomer cements cannot be used as a posterior restorative material in stress bearing areas. A new ionomer called Zirconomer, zirconia reinforced glass ionomer claims to exhibit high physical and biological properties. Aim: To assess and compare the microleakage, compressive strength, flexural strength and fluoride release from zirconomer with ketac silver. Materials & Methods: On twenty caries free premolar teeth (10 per each group), a class v cavity was restored with zirconomer and ketac silver. The microleakage was assessed using dye penetration test and stereomicroscope. The compressive and flexural strengths of these materials were measured using Instron Universal testing machine. The amount of fluoride released from the modified ionomers at pH 5 and pH 7 was estimated using Orion fluoride specific electrode. The obtained data was then subjected to statistical analysis. Results: Data was analysed using paired t-test for intergroup comparisons and unpaired t-test for intragroup comparisons. The overall microleakage (0.5±0.48) of zirconomer was significantly less (p=0.000) compared to ketac silver (1.9±0.83). Zirconomer demonstrated a significant higher compressive strength (330.25±60.14), flexural strength (33.058±2.36) than ketac silver (p= 0.000). Zirconomer demonstrated high fluoride release from day 1 to day 7 at both pH 5 and pH 7. Conclusion: Zirconomer demonstrated better physical and biological properties compared with ketac silver.

scholarly journals Hypochlorite solution for root canal irrigation that lacks a chlorinated odor

2017 ◽  
Vol 11 (02) ◽  
pp. 221-225
Author(s):  
La-ongthong Vajrabhaya ◽  
Vanida Sangalungkarn ◽  
Ratchapin Srisatjaluk ◽  
Suwanna Korsuwannawong ◽  
Chareerut Phruksaniyom
Keyword(s):  
Root Canal ◽  
Antibacterial Effect ◽  
In Vitro Study ◽  
Commercial Products ◽  
Canal Irrigation ◽  
Hypochlorite Solution ◽  
Root Canal Irrigation ◽  
Chlorine Gas ◽  
Trichloroisocyanuric Acid

ABSTRACT Objectives: This is an in vitro study to develop a formulation of a hypochlorite solution for root canal irrigation that lacks a chlorinated odor. The antibacterial effect, tissue dissolution efficacy, and the cytotoxicity of the solution were assessed in cell culture and were compared with those of commercial sodium hypochlorite (NaOCl) solutions. Materials and Methods: Trichloroisocyanuric acid (TCA) was used as the source of hypochlorite ions in solution. All required properties of the NaOCl irrigant were evaluated and compared with those of original 2.5% NaOCl solutions currently in use. Results: Our results revealed that a TCA 3.5% + 1/6 Buffer-1 solution passed the short-term stability test. Moreover, no odor of chlorine gas was detected by three independent observers. The hypochlorite ion content and pH were stable over an incubation period of 4 weeks. The new solution did not differ from commercial products in terms of the dissolution property on bovine pulpal tissue (P > 0.05). Moreover, the antibacterial effect of this solution on Enterococcus faecalis did not differ from that of the commercial products (P > 0.05). In addition, our biocompatibility analysis demonstrated no difference among the tested solutions (P > 0.05). Conclusions: According to the results of all properties tested, TCA 3.5% + 1/6 Buffer-1 could be considered an option for NaOCl irrigation with the benefit of no detectable chlorine odor.

scholarly journals Inhibition of Demineralization at Restoration Margins of Z100 and Tetric EvoCeram Bulk Fill in Dentin and Enamel

2019 ◽  
Vol 6 (2) ◽  
pp. 36
Author(s):  
Cristina Leon-Pineda ◽  
Kevin Donly
Keyword(s):  
Light Microscopy ◽  
Polarized Light ◽  
In Vitro Study ◽  
The Other ◽  
Solution Ph ◽  
Class V ◽  
The Mean ◽  
Student’S T ◽  
Student’S T Test

Recurrent caries is still considered the main reason restorations need to be replaced. There are different materials available now that promise to reduce the possibility of recurrent caries by releasing fluoride and inhibiting restoration marginal caries. The purpose of this in vitro study was to evaluate the demineralization inhibition potential of a non-fluoride-releasing resin (Z100TM 3M, St. Paul, MN, USA) and a glass containing resin-based composite (Tetric EvoCeram Bulk Fill, Ivoclar/Vivadent AG, Schaan, Liechtenstein), which contains fluoride. Class V preparations were placed on 22 premolars; the gingival margin was below the cementoenamel junction and the occlusal margin was placed above the cemento-enamel junction. Ten teeth were randomly selected to be restored with Z100 while the other 10 were restored with Tetric EvoCeram Bulk Fill. Both groups were restored following manufacturer’s instructions. All teeth had an acid resistant varnish placed within one millimeter of the preparation margins. Both groups were placed in artificial caries challenge solution (pH 4.4). At the end of the 4 days; 100 µm buccolingual sections were obtained for each tooth; these were photographed under polarized light microscopy and the demineralized areas adjacent to the restorations were measured and quantified. The mean (±S.D.) area (µm2) of demineralization from the occlusal margin (enamel) and dentin margin were: Z100 2781.889 ± 1045.213; 3960.455 ± 705.964 and for Tetric EvoCeram Bulk Fill 1541.545 ± 1167.027; 3027.600 ± 512.078. Student’s t-test indicated that there was significantly less enamel and dentin demineralization adjacent to Tetric EvoCeram Bulk Fill compared to Z100; there was significantly less demineralization in enamel compared to dentin in both Tetric EvoCeral Bulk Fill and Z100. Tetric EvoCeram Bulk Fill performed better inhibiting demineralization at restoration margins when compared to Z100 and provided better demineralization inhibition in enamel than cementum/dentin.

Effect of Different Intermediary Bases on Microleakage of a Restorative Material in Class II Box Cavities of Primary Teeth

2017 ◽  
Vol 40 (2) ◽  
pp. 82-87 ◽  
Author(s):  
Faika Y. Abdelmegid ◽  
Fouad S. Salama ◽  
Waleed M. Al-Mutairi ◽  
Saud K. Al-Mutairi ◽  
Sultan O. Baghazal
Keyword(s):  
Primary Teeth ◽  
In Vitro Study ◽  
Class Ii ◽  
Positive Control ◽  
Negative Control ◽  
The Other ◽  
Control Groups ◽  
Significant Difference ◽  
The Mean

Introduction The aim of this in vitro study was to assess and compare the effect of different intermediary bases on microleakage between tooth and a nanocomposite interface in Class II box cavities in primary teeth. Methods Standard Class II box cavities were prepared in 52 primary molars and randomly divided into 9 groups according to the intermediary base used (Multicore Flow, Fuji II LC, SDR, Smart Dentin Replacement, and Biodentine). All specimens were subjected to thermocycling and prepared for microleakage testing and evaluation. Results There was significant difference in the mean ranks of microleakage between the 9 groups, which was observed in the gingival side (p<0.0001) and the occlusal side (p<0.0001). The mean ranks microleakage was significantly higher with experimental SDR, experimental Multicore Flow, and positive control materials when compared with the other 6 groups. The microleakage mean ranks were statistically significantly lower in experimental Fuji II LC, experimental Biodentine, and all negative control groups when compared with the other 3 groups. Conclusions Microleakage is affected by the application of intermediate material. Experimental Biodentine and Fuji II LC showed the lowest microleakage while experimental SDR and experimental Multicore Flow showed the highest microleakage.

pH of Medicated Syrups–Does It Really Matter? – An in-vitro Study: Part-II

2008 ◽  
Vol 33 (2) ◽  
pp. 137-142 ◽  
Author(s):  
KL Girish Babu ◽  
Kavita Rai ◽  
Amitha Hegde
Keyword(s):  
Atomic Absorption ◽  
In Vitro Study ◽  
Vitro Study ◽  
Atomic Absorption Spectrometer ◽  
Alkaline Ph ◽  
Time Intervals ◽  
Neutral Ph ◽  
Absorption Spectrometer ◽  
Dissolution Potential

The aim of the present in vitro study was to quantify the endogenous erosive potential of the various pediatric liquid medicaments by measuring the calcium dissolution of primary enamel after immersing it for different time intervals (1min, 10 min and 8 hours) using atomic absorption spectrometer (AAS). The eight commonly used pediatric liquid medicaments which were selected had definitive endogenous calcium dissolution potential. All the medicaments showed calcium dissolution of primary enamel at alkaline pH and near neutral pH. The calcium dissolution potential of pediatric liquid medicaments when compared with their respective pH showed no statistically significant correlation. In conclusion, there was evidence of calcium dissolution from all the pediatric liquid medicaments.

Effect of Operator Experience on the Outcome of Fiber Post Cementation With Different Resin Cements

2013 ◽  
Vol 38 (5) ◽  
pp. 555-564 ◽  
Author(s):  
GM Gomes ◽  
OMM Gomes ◽  
A Reis ◽  
JC Gomes ◽  
AD Loguercio ◽  
...  
Keyword(s):  
Bond Strength ◽  
In Vitro Study ◽  
T Test ◽  
Fiber Post ◽  
Operator Experience ◽  
Fiber Posts ◽  
Glass Fiber Posts ◽  
Adhesive Cement ◽  
Push Out

SUMMARY Objectives To evaluate the influence of operator experience (dentist vs student) and cementation system (Adper Scotchbond Multi-Purpose [SBMP] + RelyX ARC [1]; Adper Single Bond 2 [SB] + RelyX ARC [2] and RelyX U100 [3]) on the push-out bond strength (BS) of fiber post to radicular dentin. Materials and Methods The roots of 48 extracted human maxillary central incisors were prepared and divided into six groups (n=8), according to combination of the above factors. Glass fiber posts were cemented in accordance with the instructions of the manufacturer of each cementation system. After water storage at 37°C for one week, the roots were cross-sectioned into six 1-mm thick slices and the push-out test was performed (0.5 mm/min). Data were statistically analyzed by two-way analysis of variance and Tukey tests (α=0.05). The BS results obtained by dentist and student for each cementation system were compared using the Student t-test (α=0.05). Results Higher BS means were observed for the expert operators, irrespective of the cementation system used (p=0.006). RelyX U100 showed the highest bond strength, but it did not differ from SBMP + RelyX ARC. The Student t-test revealed that only RelyX U100 was not affected by the operator's experience. Conclusion Within the limitations of this in vitro study, it can be concluded that the self-adhesive cement RelyX U100 showed the highest bond strength to the root canal in the student's group, and its performance was not affected by the operator's experience.

A Fluoride-containing Composite Resin—An In Vitro Study of a New Material for Orthodontic Bonding

1989 ◽  
Vol 16 (3) ◽  
pp. 207-212 ◽  
Author(s):  
P. A. Cook ◽  
C. C. Youngson
Keyword(s):  
Bond Strength ◽  
Composite Resin ◽  
Glass Ionomer Cement ◽  
In Vitro Study ◽  
The Other ◽  
Glass Ionomer ◽  
New Material ◽  
Bond Strengths ◽  
Ionomer Cement

The shear/peel bond strength of a new material, a ‘hybrid’ of a composite resin and a glass ionomer cement, was compared in vitro with the bond strengths of a composite resin and of a glass ionomer cement. The new material had a significantly greater bond strength than the other materials tested and its properties were very similar to the composite resin. Unlike the glass ionomer cement, etching of the enamel before applying the adhesive is required. The clinical indications for using this new cement are discussed.

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