scholarly journals Development of wheat plants coinoculated with rhizobium strains

Author(s):  
Wellington F. Roque ◽  
Salomão L. Guimarães ◽  
Edna M. Bonfim-Silva

ABSTRACT This study aimed to evaluate the efficiency of diaztrophic bacteria coinoculation in wheat cultivars grown in Cerrado Oxisol. A randomized block design was used, with a 13 x 3 factorial scheme and four replicates. The treatments consisted of inoculation with Azospirillum brasilense (strains AbV5 and AbV6 strains combined) and coinoculation with Bradyrhizobium japonicum (strains SEMIA 5079 and SEMIA 5080 combined, and strain BR3267), as well as Rhizobium tropici (strains MT08 and MT15) and R. leguminosarum (strain MT16) combined or in isolation, tested in wheat cultivars BRS 394, BRS 264 and BRS 254. The variables analyzed were grain nitrogen concentration and accumulation and crude protein content, 100-grain weight and total grain mass. The treatment containing the commercial cowpea inoculant showed a higher total grain mass (5.8766 g). Interaction was observed for grain nitrogen concentration, particularly for A. brasilense + MT 15 (R. tropici) and MT 15 in wheat cultivar BRS 264. Coinoculation with diazotrophic bacteria isolated from leguminous plants shows potential for use in wheat cultivars.

2017 ◽  
Vol 39 (2) ◽  
pp. 137
Author(s):  
Marielly Maria Almeida Moura ◽  
Daniel Ananias de Assis Pires ◽  
Renê Ferreira Costa ◽  
Daniella Cangussú Tolentino ◽  
João Paulo Sampaio Rigueira ◽  
...  

 This study aimed to evaluate the nutritional characteristics and quality of silages of five sorghum genotypes, namely: Volumax, AG2005E, Qualimax, BRS610 and AG2501. The study was conducted at the Experimental Farm Unimontes, in the municipality of Janaúba, state of Minas Gerais. The treatments were distributed in a randomized block design with four replicates. Data were tested by analysis of variance andmeans were compared by Scott-Knott test at a significance level of 5%. The pH values ranged from 3.93 and 4.10. Genotypes differed in all studied nutritional characteristics (p < 0.05). For the acid detergent fiber, differences were detected between the genotypes, AG2501 (35.66%), Volumax (34.89%), AG2005E (34.53%), Qualimax (32.39%) and BRS610 (33.60%). The high participation of lignin was verifiedin all genotypes except for Volumax (4.14%). All silages were within the recommended range 50-65% for in vitro dry matter digestibility and classified as having good quality, however only the AG2005E genotype met nitrogen requirements for microbial fermentation, with 7.06% crude protein. 


Author(s):  
M Alvin Rifqi Fadilla, Erwanto, Muhtarudin, dan Agung Kusuma Wijaya

The purpose of this research was to determine the effect of addition of Multinutrient Sauce (MNS) in the ration on digestibility of crude protein and crude fiber in sheep.  This research was conducted in May—July 2019 at the People's Farm of Kebagusan Village, Gedong Tataan District, Pesawaran Regency.  The digestibility analysis of crude protein and crude fiber was carried out at the Laboratory of Animal Nutrition and Feed, Animal Husbandry Department, Faculty of Agriculture, University of Lampung.  This study used a randomized block design (RBD) based on sheep body weight with six groups and three treatments, namely R0 (ration without MNS); R1 (R0 + MNS 5%); and R2 (R0 + MNS 10%).  The data obtained were analyzed using ANOVA at a 5% significance level or 1% significance, and continued with further test using Least Significant Difference Test (LSD) if there is significant results.  The results showed that the addition of 10% MNS in the ration had a significant effect (P<0.05) on increasing the digestibility of crude protein and crude fiber in sheep.   Keywords: multi nutrient sauce (MNS), digestibility of crude protein, digestibility of crude fiber, sheep.


2021 ◽  
Vol 34 (3) ◽  
pp. 692-701
Author(s):  
PAULA FABRINY MAUÉS DA SILVA ◽  
SARAH OLIVEIRA SOUSA PANTOJA ◽  
FELIPE NOGUEIRA DOMINGUES ◽  
ANIBAL COUTINHO DO RÊGO ◽  
CRISTIAN FATURI

ABSTRACT Coconut meal can be an affordable low-cost alternative to noble ingredients such as corn and soybean meal in sheep diets. The objective of this study was to evaluate the effects of coconut meal inclusions, when used as a corn and soybean meal replacement, on the intake, digestibility, and nitrogen balance of sheep diets. Twenty-four male Santa Inês lambs with body weights (BW) of 26.6 ± 3.2 kg were used in the study. The animals were housed in metabolic cages and distributed in a completely randomized block design, with three treatments and eight replications. The animals received a total mixed ratio of 30% roughage (elephant grass) and 70% concentrate. The animals were fed on two experimental diets with 11% and 22% coconut meal inclusion and a control diet (without coconut meal). Dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), and non-fibrous carbohydrate (NFC) intake as a percentage of BW were negatively influenced by the inclusion of coconut meal. The ether extract (EE) intake increased but did not differ between the 11% and 22% coconut meal diets. Furthermore, the digestibility of DM, CP, EE, and ADF did not differ between the two evaluated diets. The inclusion of coconut meal reduced NDF digestibility and increased that of NFC. The level of inclusion of coconut meal in the diet must be based on the EE content in the meal; keeping the EE intake below 0.16% of the BW helped to avoid limited intake of feed.


2018 ◽  
Vol 18 (4) ◽  
pp. 610
Author(s):  
Redempta Wea ◽  
I Gusti Komang Oka Wirawan ◽  
Bernadete Barek Koten

A research aimed to evaluate nutrients digestibility in growing local male pig fed spontaneous bioconverted-tamarind seed has been done in animal feed technology laboratory of Kupang State Agricultural Polytechnic.The research used 25 local male grower pigs with body weight around 3-6 kg. The study used a randomized block design with five treatments, i.e. R0 = ration using tamarind seeds without bioconversion, R1 = ration using spontaneous bioconversion tamarinds for 24 hours, R2 = rations using spontaneous bioconversion tamarinds for 48 hours, R3 = rations using bioconversion tamarinds spontaneous for 72 hours, and R4 = rations using spontaneous bioconversion tamarinds for 96 hours. Each treatment was repeated five times in which each of them used five pigs. Parameters observed were nutrient digestibility (dry matter, crude protein, crude fat, crude fiber, and ash). The data were analyzed by using variance and continued by Duncan’s multiple range test to determine the differences between treatments. The results showed that the time of spontaneous bioconversion of tamarind seeds increased the digestibility of tamarind seed nutrients and it was concluded that the best time of spontaneous bioconversion was for 72 hours.


Agriculture ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 154 ◽  
Author(s):  
Samantha J. Grimes ◽  
Timothy D. Phillips ◽  
Volker Hahn ◽  
Filippo Capezzone ◽  
Simone Graeff-Hönninger

The combination of consumer’s ongoing demand for chia (Salvia hispanica L.) alongside the increased demand for regionally produced food products provided the impetus for this study. Its aim was to test if a regional cultivation of new chia genotypes, which were adapted to day lengths greater than 12 hours, is feasible under Central European conditions. Therefore, three early flowering chia genotypes (Sahi Alba 914, W13.1, G8) were cultivated in a randomized block design at two experimental stations in Southwestern Germany (Ihinger Hof, Eckartsweier) over the course of two years (2015, 2016). Mean yields ranged from 100 to 1290 kg ha−1. Mucilage content ranged from 9.5% to 12.2%, while the crude protein content ranged from 17.2% to 25.0%. Crude oil content fell in the range of 30.9–33.7% and the PUFA:SAT ratio ranged from 4.0 to 9.4, whereas the omega6:omega3 ratio varied from 0.27 to 0.5. As chia seed yields surpassed yield levels obtained by their countries of origin and as quality parameters obtained, were in line with the genotypes cultivated in their countries of origin, it can be assumed that a regional chia production in Southwestern Germany offers great potential, being ecologically and economically profitable.


2017 ◽  
Vol 38 (5) ◽  
pp. 3283
Author(s):  
Doglas Batista Lazzeri ◽  
Leandro Dalcin Castilha ◽  
Patrícia Barcellos Costa ◽  
Ricardo Vianna Nunes ◽  
Magali Soares dos Santos Pozza ◽  
...  

This study aimed to determine the SID isoleucine (Ile) requirement of starting barrows fed low crude protein. Two experiments were carried out. Experiment 1: Ten crossbred barrows were used in order to determine the SID AA of the basal diet (treatment with the lowest SID Ile level used in the growth performance experiment), averaging 15.00 ± 0.27 kg of initial weight, individually housed in metabolic cages and allotted in a complete randomized design, with two treatments, five replicates and one animal per experimental unit. Treatments consisted of a basal (14.13% CP and 0.450% of SID Ile) and a free protein diet. Experiment 2: A performance experiment was carried out to determine the SID Ile requirement when using low crude protein diets. Forty crossbred barrows were used, averaging 15.00 ± 0.87 kg of initial weight and distributed in a randomized block design with five treatments (0.450, 0.520, 0.590, 0.660 and 0.730% of SID Ile) and two animals per experimental unit. The average daily gain (ADG) (P=0.049) and protein deposition (P=0.01) were affected by the studied SID Ile levels. The daily need of SID Ile was estimated at 5.9 g when considering 0.61% as the optimum level of SID Ile in the diet for an improved ADG and protein deposition.


2018 ◽  
Vol 39 (6) ◽  
pp. 2517
Author(s):  
Armindo Neivo Kichel ◽  
Luis Carlos Ferreira de Souza ◽  
Roberto Giolo de Almeida ◽  
José Alexandre Agiova da Costa

This study aimed to assess productivity and nutritional value of the tropical grasses Brachiaria brizantha cv. Piatã, Xaraés, and Marandu, Panicum maximum cv. Mombaça, and B. ruziziensis cv. Kennedy in the interseason of an integrated crop-livestock (ICL) system since alternatives are needed for forage production for animal grazing in Autumn and Winter. The experimental design was a randomized block design in a split-split plot scheme with four replications. The treatments of plots consisted of five grasses, subplots consisted of three cropping systems (monoculture, intercropping with corn and unsuppressed grass, and intercropped with corn and suppressed grass), and sub-subplots consisted of four cutting intervals of grasses (50, 90, 125, and 195 days after emergence - DAE). The experiment was carried out from February to September 2014. Dry matter (DM) productivity, obtained at 195 DAE for the three cropping systems (monoculture grass, unsuppressed and suppressed grass in intercropping), were 18.45, 7.15, and 3.05 t ha?1, respectively, and average crude protein contents of leaf blades of grasses decreased linearly between the cutting intervals of 50 to 195 DAE from 19.95 to 9.70%, respectively. Under integrated systems, the studied grasses showed better yields and nutritional quality when compared to traditional grazing systems. Panicum maximum cv. Mombaça and Brachiaria brizantha cv. Xaraés and Piatã had the highest leaf and crude protein yields when compared to Brachiaria ruziziensis cv. Kennedy and Brachiaria brizantha cv. Marandu. In terms of nutritional value, Brachiaria ruziziensis cv. Kennedy was superior to Mombaça and Xaraés grasses but had lower total dry matter yield. Finally, Xaraés, Piatã and Mombaça grasses are recommended choices ICL systems when fodder grass production is the goal.


2018 ◽  
Vol 28 (3) ◽  
pp. 213
Author(s):  
Eko Marhaeniyanto ◽  
Sri Susanti

The aim of this research was to study the in vitro ruminal fermentability of supplementation of <em>Gliricidia sepium, Moringa oleifera</em>, Lamk (<em>MOL</em>), <em>Ceiba pentandra</em> and <em>Paraserianthes falcataria </em>leaf meal in concentrate feed to be tested on sheep.<strong> </strong>This research with the experimental methods was arranged in Randomized Block Design (RBD) with 6 treatments and 3 groups. The treatment feed tested consisted of concentrate without leaves and concentrate with leaf meal supplementation. Crude protein content (CP) concentrates were prepared 16%, 18% and 20%. Supplementation uses a mixture of <em>Gliricidia sepium, MOL</em>, <em>Ceiba pentandra</em> and <em>Paraserianthes falcataria </em>leaf meal in concentrate feed (1: 1: 1: 1) as much as 10%, 20% and 30%. The measured variables were  degradation of dry matter (DDM) and degradation of organic matter (DOM), gas production rate, microbial biomass and NH<sub>3</sub> concentration. The use of mixed leaf meal in concentrate feed resulted in in vitro DDM as well as concentrate feed without leaf meal supplementation. Supplementation of leaf meal in concentrate feed as much as 30% with CP 20% resulted in a decrease in the value of fermentability. The supplementation of leaf meal in concentrate feed as much as 20% in concentrate feed with CP 18% produced the best fermentability value. Suggested for in-vivo trials on sheep using 18% protein concentrate feed by utilizing <em>Gliricidia sepium, MOL</em>, <em>Ceiba pentandra</em> and <em>Paraserianthes falcataria </em>leaf meal in concentrate feed as cheap protein sources.


Author(s):  
Rijanto Hutasoit ◽  
Simon Ginting ◽  
Juniar Sirait ◽  
Andi Tarigan

The cultivation of mulberry as forages is scarce in Indonesian feeding system for ruminant production, although many studies have indicated  its nutritional potential as animal feed especially ruminants. This study was aimed to investigate the effects spacing of plant and cutting age on productivity and chemical composition of some species mulberry plants. The experiment was conducted in a randomized block design with factorial treatments. The factors species were (M. indica cv kanva, M. nigra, M. cathyana, and M. Multicaulis), the spacing of plants (1 x 0.5 m, 1 x 1 m and 1 x 1.5 m) and cutting ages (210, 240, and 270 days after planting). The results showed that species, M. multicaulis and M. cathyana are the highest growth, using a spacing of 1 x 0.5 m with cutting age 270 days after planting. Dry matter production highest (P < 0,05) in M. Cathyana at spacing of 1 x 0.5 with cutting age 270 days after planting. The ratio of leaves and stems of plants are affected by age, the highest proportions of leaf  is obtained at the cutting age of 210 days. Chemical composition were highest in M.multicaulis at the age of 270 days. The highest content of  organic matter (OM) were found in M.Kanva and not different with M. Nigra at the age of 270 day after planting. In treatment of cutting ages have no effect (P < 0.05) on the Crude protein (CP), ADF, NDF, lignin and tannin content. The average content of CP obtained gained 20.69%, 32.38% ADF, NDF 33.99%, 12.45% lignin and tannin 0.3%. Concluded that mulberry plants with a plant spacing of plant 1 x 0.5 and 270 days after plant have more advantages for use as animal feed.


2020 ◽  
Vol 3 (2) ◽  
pp. 48-60
Author(s):  
Billy Andreas Putra ◽  
Tengku Boumedine Hamid Zulkifli ◽  
Yunida Berliana ◽  
Octanina Sari Sijabat

Introduction: The purpose of this study was to determine the dosage of inorganic fertilizers, soaking time of the inoculation of the legume bacteria strains which were suitable for the growth and yield of peanut in tidal lowland. Materials and Methods: This research was conducted at tidal lowland in Sei Ular Village, Secanggang sub-District, Langkat District, North Sumatra from September to December 2019. The research used randomized block design in factorial. The first factor is the dose of inorganic fertilizers consisting of P0 = un-fertilizer (control), P1= urea 50 kg.ha-1 + TSP 180 kg.ha-1 + KCl 50 kg.ha-1, P2= urea 75 kg.ha-1 + TSP 205 kg.ha-1 + KCl 75 kg.ha-1, P3= urea 100 kg.ha-1 + TSP 230 kg.ha-1 + KCl 100 kg.ha-1. The second factor was the soaking time of the Bradyrhizobium japonicum bacterial strain consisting of T0= un-soaking time, T1= soaking time for 3 minutes, T2= soaking time for 6 minutes, and T3= soaking time for 9 minutes. Parameters were analyzed using ANOVA and BNJ analysis 5%.· Results: The application of urea 50 kg.ha-1 + TSP 180 kg.ha-1 + KCl 50 kg.ha-1 significantly increased the plant height, number of leaves, and root volume and showed the highest number of active nodules, dry pods.plot-1 and seed weight.plot-1 compared to other doses. The seed soaking time with the Bradyrhizobium japonicum strain and their interactions showed insignificant differences in all parameters of peanut plants in tidal lowland.


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