scholarly journals Soaking curve and effect of temperature on the germination of daisy seeds

2012 ◽  
Vol 30 (2) ◽  
pp. 312-316 ◽  
Author(s):  
Rogério G Pêgo ◽  
José Antônio S Grossi ◽  
José Geraldo Barbosa
Keyword(s):  
Seed Germination ◽  
Effect Of Temperature ◽  
Distilled Water ◽  
Germination Test ◽  
Tukey Test ◽  
Germination Process ◽  
Randomized Experimental Design ◽  
Chrysanthemum Leucanthemum ◽  
Triphasic Pattern

The soaking curve and the effect of temperature on the germination of daisy seeds (Chrysanthemum leucanthemum) were characterized in this study. To determine the soaking curve, four samples of 0.5 g of seeds were soaked in germitest paper moistened with distilled water and maintained in germinator at 25ºC. The seeds were weighed in periods of 0, 3, 6, 9, 12, 24, 36, 48, 60, 72, 84 and 96 hours using a precision digital balance of 0.0001 g. A triphasic pattern germination curve was adjusted, allowing the determination of the beginning and duration of the phase II of the germination process. The germination test was carried out with four replications of 50 seeds disposed in "Gerbox" boxes and placed in germinators at the temperatures of 20, 25, 30 or 20-30ºC. A completely randomized experimental design was used with four replications of 50 seeds. The data were submitted to the analysis of variance and the averages were compared by the Tukey test, at 5% of probability. For analysis of accumulated seed germination, regressions were adjusted based on period of experiment. The seeds presented a triphasic pattern of germination and the phases I and II lasted 12 and 48 hours, respectively. The best temperature for the germination of the seeds is 25ºC. The temperature of 30ºC promoted the thermoinhibition of germination and increased the dead and dormant seeds and abnormal seedlings.

scholarly journals Seed Germination and Physiological Response of Sunflower (Helianthus annuus L.) Cultivars under Saline Conditions

2018 ◽  
Vol 75 (1) ◽  
pp. 4
Author(s):  
Carmen BEINSAN ◽  
Renata SUMALAN ◽  
Giancarla VELICEVICI ◽  
Adriana CIULCA ◽  
Radu SUMALAN
Keyword(s):  
Seed Germination ◽  
Helianthus Annuus ◽  
Dry Matter ◽  
Saline Stress ◽  
Free Proline ◽  
Radicle Growth ◽  
Helianthus Annuus L ◽  
Control Seed ◽  
Germination Process

The purpose of the experiment was to highlight the germination of sunflower seeds affected by the presence of saline stress and the identification of tolerant genotypes. The biological material was represented by sunflower cvs. (Helianthus annuus L.): Coril, Select, Santiago and Fundulea-206. To simulate the saline conditions, germination solutions of sodium chloride (NaCl) were used with concentrations corresponding to the osmotic pressures -6 and -10 atm and the control seed hydration was performed with distilled water. Determination of seed germination, growth of seedling, percentage of plumules dry matter, chlorophyll content and free proline were performed. The experimental data obtained suppose the existence in the assimilation apparatus of sunflowers seedling subjected to stress a competitive chlorophyll/free proline biosynthesis processes. The experimental results regarding the effect of salinity on seed germination and seedling growth revealed important differences between genotypes. The radicle growth in the germination process were strongly affected by saline excess, with significant differences between cultivars. Saline stress results in significant reductions in the amount of chlorophyll, and high levels of free proline. It can be observed that with the increase of the stress level the percentage of the dry matter increases, indicating an accentuated water deficit.

Effect of Priming Techniques on Seed Germination Characteristics of C. Maxima Duch

2011 ◽  
Vol 474-476 ◽  
pp. 36-39 ◽  
Author(s):  
Yong Dong Sun ◽  
Xin Zheng Li ◽  
He Lian Yang ◽  
Li Sun
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Seed Priming ◽  
Distilled Water ◽  
Nacl Solution ◽  
Peg 6000 ◽  
Germination Process ◽  
Germination Characteristics ◽  
Successful Technique

The present study was conducted to investigate the effect of seed priming techniques on germination characteristics of C. maxima Duch. cultivar (Beiguan). Treatments were combinations of 3 levels of priming (distilled water, NaCl and PEG6000) and non-priming (control) with 3 replications. Concentrations of NaCl solution were 50, 100, 150, 200 mmol•L-1, and polyethylene glycol (PEG) 6000 were 10%, 20%, 30%, 40%, respectively. Seeds were primed using the above priming materials for 24 hours at 20°C in the dark, respectively. The results showed that different priming techniques could have various effects on germination of Beiguan seeds. Hydropriming (distilled water), NaCl priming and PEG6000 priming (10%) all improved the germination characteristics of Beiguan, compared to the control. NaCl priming was more effective than hydropriming and PEG6000 priming and was the most successful technique in this study. These findings indicated that seed priming techniques could accelerate germination process and were simple and cheap, we should propose these methods to farmers.

scholarly journals The Effect of Seed Sources Variation and Presowing Treatments on the Seed Germination ofAcacia catechuandElaeocarpus floribundusSpecies in Bangladesh

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Niamjit Das
Keyword(s):  
Seed Germination ◽  
Hot Water ◽  
Cow Dung ◽  
Germination Test ◽  
Rural Bangladesh ◽  
Seed Sources ◽  
Germination Process ◽  
Germination Success ◽  
Acacia Catechu ◽  
Source Variation

The seed germination of seed sources and presowing treatments ofAcacia catechuandElaeocarpus floribundusseeds were conducted in the nursery of Bangladesh Agricultural University. The seeds were collected from matured and healthy trees from four different locations in Bangladesh and treated with six presowing methods. The germination test was conducted in polybags with a mixture of topsoil and cow dung in a ratio of 3 : 1. The results of ANOVA showed no significant differences among seed sources but statistically significant differences among the presowing treatments for both species. Thus the presowing methods affected the germination process of seeds, and then the highest germination success was found to be 91.26% in hot water (80°C for 10 min), treatment inAcacia catechuand the highest germination success (89.81%) ofElaeocarpus floribunduswas found in H2SO4treatment followed by 86.35% and 78.42% in treatments with hot water (100°C for 12 min) and scarification. The study also revealed that the interactions between seed source variation and presowing methods effect significantly differed in seed germination percentages. Therefore, it is concluded that hot water treatment can be suggested on seed germination of both species for developing nurseries and rural Bangladesh.

scholarly journals Substrate but not seed size affects seed germination of Dypsis onilahensis (Jum. & H. Perrier) Beentje & J. Dransf

2015 ◽  
Vol 36 (6Supl2) ◽  
pp. 4193
Author(s):  
Petterson Baptista da Luz ◽  
Thalita Neves Marostega ◽  
Bruno Nicchio ◽  
Leonarda Grillo Neves
Keyword(s):  
Seed Size ◽  
Germination Percentage ◽  
Palm Tree ◽  
Tukey Test ◽  
Germination Process ◽  
Germination Speed ◽  
Randomized Experimental Design ◽  
Sowing Seed ◽  
Substrate Types ◽  
Germinated Seeds

Dypsis onilahensis, commonly known as sibara palm, is a palm tree with enormous ornamental potential. Commercial propagation occurs through seeds, but few studies have evaluated the factors that affect the germination process of this species. Thus, we aimed at evaluating the effects of seed size and substrate on D. onilahensis germination. We used a completely randomized experimental design and a 2 x 3 factorial scheme with two seed sizes (> 15 mm and < 15 mm in diameter) and three substrate types (sand, Plantmax®, and vermiculite), with four replications of 15 seeds. We analyzed germination percentage (G%), number of germinated seeds (NGS), and germination speed index (GSI). Data underwent variance analysis, and means were compared by the Tukey test at 5% probability. Germination began 73 days after sowing. Seed size had no effect on germination. Among substrates, vermiculite showed the best results for GSI, NGS, and G%, followed by sand and then by Plantmax®. Our study provides the first data on factors that affect the germination of this palm tree species.

Overcoming Dormancy and Influence of Light on the Physiological Quality of Senna cana (Fabaceae) Seeds

2019 ◽  
pp. 1-9
Author(s):  
Jordânia X. Medeiros ◽  
Ana L. P. Feliciano ◽  
Valderez P. Matos ◽  
Girlânio H. Silva ◽  
Yana S. Lopes ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Native Species ◽  
Red Light ◽  
Distilled Water ◽  
Light Regimes ◽  
Germination Process ◽  
Physiological Quality ◽  
Mean Germination Time

Environmental factors affect the germination process, like the presence of seed coat and the quality of light; these informations are still scarce for many native species from Brazil, especially for Senna cana, which there are no adequate standards and methodologies to be used in germination tests. The aim of this research was to recommend adequate pre-germinative treatment(s) to overcome seed dormancy, and determine the degree of influence of different light regimes in seed germination of S. cana. Two experiments were carried out: T1-evaluation of different methods of dormancy overcoming (intact seeds (control), T2-imbibition of the intact seeds for 24 hours (in distilled water), T3-scarified seeds with sandpaper n° 100 in the hilum opposite region, T4-scarified seeds with sandpaper n° 100 in the region the hilo opposite region and imbibition in water (in distilled water) for 24 hours; T5-imbibition in water at 80°C); 2-Influence of light quality on seed germination and vigor (white light, red light, far red light and absence of light). The evaluated parameters were: first germination count, percentage of germination, IVG (Germination speed index), MGT (Mean germination time). Treatments were compared by Tukey at 5% probability. The best method for overcoming seed dormancy was mechanical scarification with sandpaper nº 100. The germination of the seeds of S. cana can be classified as indifferent to the luminosity, despite germinative response be greater under white and red light.

scholarly journals STANDARDIZATION OF GERMINATION TEST AND RESPONSE TO NACL SALT STRESS IN Toona cliliata SEEDS

FLORESTA ◽  
2015 ◽  
Vol 45 (4) ◽  
pp. 845 ◽  
Author(s):  
Letícia Ramon De Medeiros ◽  
Manoela Andrade Monteiro ◽  
Patrícia Migliorini ◽  
Marilia Lazarotto ◽  
Lilian De Tunes
Keyword(s):  
Salt Stress ◽  
Sodium Chloride ◽  
Seed Germination ◽  
Dry Matter ◽  
Distilled Water ◽  
Germination Test ◽  
Light Regimes ◽  
Seedling Length ◽  
Toona Ciliata ◽  
Germinated Seeds

AbstractThe objective of this study was to evaluate the germination of Australian cedar seeds (Toona ciliata M. Roem) in three different substrates and two photoperiods and evaluate the effect of salt stress with sodium chloride (NaCl) on the viability and vigor of seeds, conducting two experiments. The first experiment was conducted in a constant temperature room at 25 C and two light regimes 12h 24h white light; and three substrates: sand, between paper and on paper. Evaluations were performed on the seventhand twenty-first day after sowing. Results were expressed as percentage of Normal Seedlings, Abnormal Seedlings and Dead Seeds, Number of True Leaves, Fresh, Dry Matter, Seedling Length and Root. The second experiment used constant 25 C and photoperiod of 12 hours light. With treatments T1 (0mM: distilled water); 25mMNaCl T2; T3 50mMNaCl; T4 and T5 100mMNaCl 75mMNaCl. We evaluated PCG, G, IVG, CP, CR. The photoperiod of 24h light, does not influence the final seed germination and the use of paper on substrate provided the highest percentage of germinated seeds. As to the effect of salt stress, the concentration of 50mMsalt, caused damage to the development of seedlings.ResumoPadronização do teste de germinação e resposta ao estresse salino por NaCl em sementes de Toona ciliata. O objetivo do trabalho foi avaliar a germinação de sementes de cedro australiano (Toona ciliata M. Roem) em três substratos com dois fotoperíodos e avaliar o efeito do estresse salino com cloreto de sódio (NaCl) na viabilidade e vigor de sementes. O experimento I foi conduzido em ambiente com temperatura constante a 25 ºC e dois regimes de luminosidade, 12h de 24h de luz branca; três substratos: areia, entre papel e sobre papel. As avaliações foram realizadas no sétimo e vigésimo primeiro dia após a semeadura. Os resultados expressos em porcentagem de Plântulas Normais, Plântulas Anormais e Sementes Mortas, número de Folhas Verdadeiras, Massa Fresca, Massa Seca, Comprimento de Plântula e Raiz. O experimento II utilizou temperatura constante a 25 ºC e fotoperíodo de 12 horas luz. Com os tratamentos T1 (0 mM: água destilada); T2 25 mMNaCl; T3 50 mMNaCl; T4 75 mMNaCl e T5 100 mMNaCl. Avaliando-se PCG, G, IVG, CP e CR. O fotoperíodo de 24h de luz, não influencia na germinação e o uso do substrato sobre papel proporcionou a maior germinação. Quanto ao efeito do estresse salino, a concentração de 20 mM de sal, prejudicou o desenvolvimento das plântulas.Palavras-chave: Sementes florestais; análise de sementes, vigor, salinidade.

scholarly journals Dynamics of the rangpur lime seed germination test conducted under different temperatures

2019 ◽  
Vol 41 (3) ◽  
pp. 344-351
Author(s):  
Marizangela Rizzatti Ávila ◽  
Juliana Barbosa ◽  
Nelson da Silva Fonseca Júnior ◽  
Getúlio Takashi Nagashima ◽  
Carolina Maria Gaspar de Oliveira ◽  
...  
Keyword(s):  
Experimental Design ◽  
Seed Germination ◽  
Standard Procedure ◽  
Time Interval ◽  
Germination Test ◽  
Rangpur Lime ◽  
Germination Process ◽  
Different Temperatures ◽  
Time Required ◽  
Seed Germination Test

Abstract: Adoption of a standard procedure to perform the germination test allows comparison of results between laboratories. Thus, the aim of this study was to evaluate the germination process of rangpur lime (Citrus limonia Osbeck) seeds conducted under different temperatures. The germination test was carried out in five seed lots under three temperatures (25, 30 and 35 °C). The dynamics of the germination process was studied through the following variables: germination (%), time to first germination (days), time to last germination (days), time interval between first and last germination (days), time required to reach 50% of germination (days), mean speed of germination (day-1), synchrony of the germination process (dimensionless), and uncertainty of the germination process (bits). The experimental design was completely randomized, and analysis of variance was performed in a 5 (lots) x 3 (temperatures) factorial arrangement, with four replicates of each treatment. Means were compared using Tukey’s test (p < 0.05). The germination process in rangpur lime seeds is slow, homogenous, and asynchronous; and the temperature of 30 °C is the most favorable for conducting the germination test, with the first count at 21 days and the last at 30 days.

scholarly journals DÉFICIT HÍDRICO E A GERMINAÇÃO DE SEMENTES DE HÍBRIDOS DE MILHO

Nativa ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 240-246
Author(s):  
Ivanildo Guilherme Henrique ◽  
Rafael Orlandini Bosqueiro ◽  
Rafael Massaru Kotsubo ◽  
Silvia de Carvalho Campos Botelho
Keyword(s):  
Zea Mays ◽  
Seed Germination ◽  
Water Deficit ◽  
Seed Vigor ◽  
Experimental Unit ◽  
Water Restriction ◽  
Maize Seed ◽  
Germination Process ◽  
Randomized Experimental Design ◽  
Influence Of Water

Considerando a importância da água no processo de germinação de sementes, as condições adversas de umidade afetam diretamente o processo germinativo. Para tanto, objetivou-se avaliar a influência do deficit hídrico na germinação e vigor de sementes de dois genótipos comerciais de milho, induzido por diferentes potenciais osmóticos. Utilizou-se delineamento experimental inteiramente casualizados (DIC), arranjado em esquema fatorial duplo 2x5, com quatro repetições, avaliando híbridos de milho (DKB255PRO3 e DKB390PRO2), submetidos a germinação sob condições de déficit hídrico, em cinco níveis de potencial osmótico (0, -0,6, -1,2, -1,8 e -2,4 MPa). Cada unidade experimental constituiu-se por um rolo de papel germitest contendo 50 sementes. Utilizou-se NaCl como agente osmótico indutor de deficiência hídrica as sementes. Avaliou-se a germinação, vigor de sementes e o desenvolvimento inicial das plântulas, em termos de comprimento e biomassa. O déficit hídrico, simulado com solução de cloreto de sódio, provoca redução no desempenho de sementes de híbridos de milho. Os genótipos de milho respondem diferentemente quanto a tolerância a condições de estresse provocado por indisponibilidade de água durante a germinação. O híbrido DKB390PRO2 apresenta germinação e crescimento de plântulas superior quando submetido a diferentes níveis de potencial osmótico de NaCl. Palavras-chave: viabilidade de sementes; restrição hídrica; Zea mays.   Effect of water deficit on the maize seed germination   ABSTRACT: Considering the importance of water in the seed germination process and knowing that conditions will not always be the most adequate to the germination process, the objective was to evaluate the influence of water deficit on germination and vigor in seeds of different commercial corn genotypes induced by different osmotic potentials. A completely randomized experimental design (D.I.C) was used, arranged in a 2x5 double factorial scheme, with four replications. Each experimental unit was constituted by a roll containing 50 seeds, made in germitest paper. Two maize seed hybrids (DKB255PRO3 and DKB390PRO2) were evaluated, submitted to germination under water deficit conditions, with levels 0; -0,6. -1,2; -1,8 and -2,4 MPa of osmotic potential. NaCl was used as osmotic agent inducing water deficiency in seeds. Germination, seed vigor and initial seedling development were evaluated in terms of length and biomass. The water deficit simulated with sodium chloride solution, causes a reduction in the performance of maize hybrids seeds. Corn genotypes respond differently to tolerance to stress conditions caused by unavailability of water during germination. The hybrid DKB390PRO2 presents superior germination and seedling growth when submitted to different levels of osmotic NaCl potential. Keywords: viability; water restriction; Zea mays.

scholarly journals DORMANCY OVERCOMING AND PRECONDITIONING IN Mimosa caesalpiniifolia Benth. SEEDS

2020 ◽  
Vol 33 (3) ◽  
pp. 720-727
Author(s):  
HOHANA LISSA DE SOUSA MEDEIROS ◽  
CLARISSE PEREIRA BENEDITO ◽  
NADJAMARA BANDEIRA DE LIMA DANTAS ◽  
JORGE RICARDO SILVA DO COUTO JÚNIOR ◽  
LUIRLA BENTO RAMALHO
Keyword(s):  
Sulfuric Acid ◽  
Experimental Design ◽  
Filter Paper ◽  
Dry Weight ◽  
Shoot Length ◽  
Hot Water ◽  
Distilled Water ◽  
Cost Of Treatment ◽  
Tukey Test

ABSTRACT Seeds of ‘sabiá’ (Mimosa caesalpiniifolia Benth.) have coat dormancy, which hampers the absorption of water and oxygen, thereby delaying germination. Thus, this study aimed to evaluate methods for overcoming dormancy associated with priming on germination and vigor of seeds of ‘sabiá’, considering the efficiency, practicality and cost of treatment. The experimental design was completely randomized, in a 4 x 5 factorial scheme (four methods for overcoming dormancy x five types of priming), constituting 20 treatments with four replicates of 25 seeds. The methods for overcoming dormancy used were tip removal, seed immersion in hot water at 100 °C for three minutes, immersion in sulfuric acid for 10 minutes and intact seeds. For the determination of physiological conditioning of seeds, the imbibition curve with the different priming agents was constructed. Priming was done on filter paper moistened with solutions of mannitol at the potentials of -0.2 MPa (16 hours), -0.4 MPa (24 hours) and -0.6 MPa (36 hours) and only with distilled water for the hydropriming (12 hours) and seeds without priming. The variables analyzed were first count of germination, germination, root length, shoot length and dry weight of seedlings. The data were subjected to analysis of variance by F test and Tukey test at 5% probability. The dormancy of M. caesalpiniifolia seeds should be overcome with the use of hot water (100 °C) for three minutes, without the need for priming.

scholarly journals Water absorption and improvement of methods for electrical conductivity test in acacia seeds

2016 ◽  
Vol 64 (4) ◽  
Author(s):  
Daniel Luiz de Oliveira ◽  
Oscar Jose Smiderle ◽  
Pollyana Priscila Schurtz Paulino ◽  
Aline Das Graças Souza
Keyword(s):  
Electrical Conductivity ◽  
Water Absorption ◽  
Distilled Water ◽  
Germination Test ◽  
Germination Speed ◽  
Water Container ◽  
The Times ◽  
Old Trees ◽  
Plastic Containers

It was intended in this work to characterize the water absorption standard after seed dormancy break and establishing the amount of water, container size and the need of breaking the tegument dormancy for the application of the electrical conductivity test in small and large seeds of A. mangium with different ages established in poor yielding-capacity soils on savannah areas of Roraima. The seeds were collected on trees 10, 8 and 6 years old, afterwards, classified into large and small, constituting six lots. Germination test with four replications of 50 seeds for each lot on germitest® paper maintained on gerbox at 25°C was carried out. Imbibition was verified with weighing in the times of 0, 2, 5, 8, 12, 16, 24, 36, 48, 60, 72, 84, 96 and 120 hours with the seeds placed between papers. The electrical conductivity test consisted of three experiments, distinguished by the amount of water utilized and by the size of the container in which the seeds were immersed. Seeds of A. mangium coming from trees over 10 years old presented increased germination percent and germination speed than seeds of six-year old trees. Small seeds present increased electrical conductivity and water absorption till 120 hours relative to large seeds. The immersion of seeds of A. mangium in 40 mL of distilled water into 180-mL plastic containers, after dormancy break, is indicated for the determination of electrical conductivity.

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