scholarly journals Evaluation of oxidative stress in mice subjected to aerobic exercise

2012 ◽  
Vol 27 (8) ◽  
pp. 544-551 ◽  
Author(s):  
Mônica Cruvinel de Lima ◽  
Guido Marks ◽  
Iandara Schettert Silva ◽  
Baldomero Antonio Kato da Silva ◽  
Lourdes Zélia Zanoni Cônsolo ◽  
...  

PURPOSE: To evaluate the influence of aerobic exercise on oxidative stress in mice. METHODS: The study included twenty female mice Mus musculus-Swiss divided into two groups: sedentary control (GA) and exercise (GB), each containing ten animals. All animals underwent an adaptation period of seven days isolated in individual boxes. After this period, the animals in the exercise group (GB) were trained in angled running wheel with circumference of 25 cm assembled on an articulated axle during five minutes for three consecutive days. On the fourth day, they underwent an exercise program of one session lasting 45 minutes. The evaluation of oxidative stress was performed by determining the levels of malondialhyde derived of lipid peroxidation by the TBA method. The samples were read in a spectrophotometer at 535 nm. RESULTS: No significant difference was observed in the intergroup comparison of MDA levels in the tissues evaluated. A significant difference was observed in the intragroup comparison of MDA levels in the control group (p = 0.0201).The Tukeys' post hoc test indicated significantly lower values of MDA in the smooth muscle in relation to plasma. In the analysis of variance in the exercise group, a significant difference between tissues (p = 0.0009), with significantly lower values in the smooth muscle in relation to plasma (p<0.001) and higher in striated muscle in relation to smooth muscle (p<0.05) was observed. CONCLUSION: There was no change in the analysis of oxidative stress in mice which were undergone a single session of aerobic exercise.

2021 ◽  
Vol 42 (Supplement_1) ◽  
Author(s):  
S Lopes ◽  
J Mesquita-Bastos ◽  
M Teixeira ◽  
D Figueiredo ◽  
J Oliveira ◽  
...  

Abstract Introduction Resistant hypertension is a major challenge of modern cardiovascular medicine, as it is a puzzling problem without a clear solution. Exercise training clearly reduces blood pressure (BP) and oxidative stress in patients with hypertension, however evidence is limited regarding resistant hypertension. Purpose To determine the effect of an aerobic exercise training program in BP, angiotensin II and oxidative stress in patients with resistant hypertension. Methods EnRicH is a prospective, two-center, single-blinded, randomized controlled trial with a parallel two-arm group. Sixty patients with resistant hypertension were randomly assigned in a 1:1 ratio to undergo a 12-week aerobic exercise training program (exercise) or usual care (control). The powered primary efficacy measure was 24-hour ambulatory systolic BP change from baseline. Secondary outcome measures included daytime and nighttime ambulatory BP, office BP, cardiorespiratory fitness, and oxidative stress and inflammatory biomarkers: Interferon-gamma (IFN-y), Angiotensin II, vascular endothelial growth factor (VEGF), and superoxide dismutase (SOD). Results Fifty-three patients (exercise n=26, control n=27) completed the study. Patients were mainly women (54.7%), with an office BP of 140.7±15.9/84.2±9.4 mm Hg and taking an average of 4.6 antihypertensive medications (median, 5; range, 3 to 7). At baseline, no differences were found between groups for the study outcomes and patient characteristics. Ambulatory systolic BP was reduced −7.1 mm Hg (95% CI, −12.8 to −1.4; P=0.015) in the exercise group (127.4±12.2 to 121.2±12.2, p=0.007) compared to control group (126.1±17.2 to 126.9±15.2, p=514) over 24-hour. In addition, 24-hour ambulatory diastolic BP (−5.1 mm Hg, −7.9 to −2.3, P=0.001), daytime ambulatory systolic (−8.4 mm Hg, −14.3 to −2.5, P=0.006), and diastolic BP (−5.7 mm Hg, −9.0 to −2.4, P=0.001) were also reduced in the exercise group compared to the control group. There were no differences in the change of nighttime ambulatory BP between groups. Cardiorespiratory fitness improved in the exercise group by 14% (4.7 ml.kg-1.min-1, P&lt;0.001), while it remained unchanged in the control group (−0.37 ml.kg-1.min-1, P=0.442). A significant between-group difference in favor of exercise group was found for IFN-y (−4.3 pg/mL, 95% CI: −7.1 to −1.5; P=0.003), Angiotensin II (−157.0 pg/mL, 95% CI: −288.1 to −25.9; P=0.020), VEGF (10.53 pg/mL, 95% CI: 0.60 to 22.54; P=0.035), and SOD (0.35 pg/mL, 95% CI: 0.10 to 0.58; P=0.009). Conclusions A 12-week moderate intensity aerobic exercise program reduced ambulatory BP, angiotensin II and oxidative stress in patients with resistant hypertension. The antihypertensive effects of exercise in patients with resistant hypertension may be mediated by positive changes in oxidative stress and inflammatory biomarkers. FUNDunding Acknowledgement Type of funding sources: Foundation. Main funding source(s): European Union through European Regional Development Fund – Operational Competitiveness Factors Program (COMPETE)Portuguese Government through FCT - Foundation for Science and Technology


2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Muhammed Emre Karaman ◽  
Cengiz Arslan ◽  
Mehmet Ferit Gürsu

Abstract Objectives The number of studies examining the circulating level change of betatrophin and cartonectin in metabolic syndrome applying different loads of exercise is limited. The purpose of the present study was to investigate how different loads of exercises regulate the betatrophin and cartonectin levels in metabolic syndrome induced rats. Methods A total of 24 Wistar-Albino male rats were used in the study. Rats were divided into four groups as follows; G1: control group (fed with standard diet and tap water), G2: metabolic syndrome group (without exercise application), G3: metabolic syndrome + aerobic exercise group (aerobic exercise applied), G4: metabolic syndrome + anaerobic exercise group (anaerobic exercise applied). Betatrophin and Cartonectin levels were determined by ELISA method in serum samples. Results There was a statistically significant difference in betatrophin levels between the groups and this differentiation was caused by G2 (p <0.05). Cartonectin levels were not significantly different between groups (p> 0.05). Conclusions It can be concluded that anaerobic exercises have more positive effects on glucose balance in metabolic syndrome than aerobic exercises, and by regulating betatrophin levels, anaerobic exercises indicate this effect.


2018 ◽  
Vol 39 (06) ◽  
pp. 413-418 ◽  
Author(s):  
Guanghui Liu ◽  
Jiasheng Zhao ◽  
Yang Cao

AbstractPhysical exercise increase is confirmed as a fundamental treatment for hypercholesterolemia patients, but the effect on the arterial stiffness and oxidative stress is still unclear. 74 hypercholesterolemia patients were divided into 2 groups. The exercise group received dietary recommendations and a 3-month exercise program, prescribed according to their first cardiopulmonary exercise tests(CPET), while the control group only received dietary recommendations. All patients underwent blood tests, CPET and brachial-ankle pulse wave velocity (baPWV) studies at enrollment and at 3 months’ follow-up. At the end of 3 months, there was no change in total cholesterol (TC) level in either group. However, in the exercise group, we found maximal exercise parameters increased and baPWV values declined. Meanwhile, there were significant changes in the level of malondialdehyde, 8-isoprostane-F2α and superoxide dismutase. Moreover, the change of baPWV was positively correlated with the change of 8-isoprostane-F2α (r=0.36, P<0.01). In the control group, no change in baPWV or oxidative biomarker was observed. Our findings suggested that regular aerobic exercise could lessen arterial stiffness in hypercholesterolemia patients, even in the context of no obvious TC decrease. During this process, favorable adjustment in oxidative stress might be an important pathway, which remains to be further explored.


2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Walid Kamal Abdelbasset ◽  
Shereen H. Elsayed ◽  
Gopal Nambi ◽  
Saud M. Alrawaili ◽  
Tamer E. Elnegamy ◽  
...  

Objective. Limited studies have assessed the effect of moderate-intensity continuous aerobic exercise on hepatic fat content and visceral lipids in hepatic patients with diabesity. This study was designed to evaluate hepatic fat content and visceral lipids following moderate-intensity continuous aerobic exercise in hepatic patients with diabesity. Design. A single-blinded randomised controlled trial. Methods. Thirty-one diabetic obese patients with nonalcoholic fatty liver disease were recruited into this study. The patients were randomly classified into exercise and control groups, fifteen patients in the exercise group and sixteen patients in the control group. The exercise group received an 8-week moderate-intensity continuous aerobic exercise program with standard medical treatment, while the control group received standard medical treatment without any exercise program. Hepatic fat content and visceral lipids were assessed before and after intervention at the end of the study. Results. Baseline and clinical characteristics showed a nonsignificant difference between the two groups (p>0.05). At the end of the intervention, the aerobic exercise showed significant improvements (serum triglycerides and low-density lipoproteins (LDLs), p≤0.002, total cholesterol, p=0.004, visceral fats, p=0.016, glycated hemoglobin (HbA1C), p=0.022, high-density lipoproteins (HDLs), p=0.038, alanine transaminases (AL), p=0.044, intrahepatic triglyceride and HOMA-IR, p=0.046, and body mass index (BMI), p=0.047), while the control group showed a nonsignificant difference (p>0.05). The postintervention analysis showed significant differences in favor of the aerobic exercise group (p<0.05). Conclusions. Moderate-intensity continuous aerobic exercise reduces the hepatic fat content and visceral lipids in hepatic patients with diabesity. Recommendations should be prescribed for encouraging moderate-intensity aerobic exercise training, particularly hepatic patients with diabesity.


2014 ◽  
Vol 17 (2) ◽  
pp. 214-221 ◽  
Author(s):  
Yueh-Min Liu ◽  
Yu-Chu Chung ◽  
Jung-San Chang ◽  
Mei-Ling Yeh

Previous studies have concluded that exercise training is beneficial to patients on hemodialysis (HD). Results, however, have shown that differences in the type, intensity, and frequency of physical exercise lead to variability in its effects on physical functional performance and depression. Further research is thus warranted. Our aim was to evaluate the effects of aerobic exercise on physical functional performance and depression during HD. Using a pretest–posttest control group design, we recruited HD patients and nonrandomly assigned them to an exercise group ( n = 13) that completed a 12-week aerobic exercise program during HD or a control group ( n = 11) that did no exercise during HD. The primary outcome measures were physical functional performance, as evaluated by the 6-min walk test and the sit-to-stand test, and depression, as evaluated by the Beck Depression Inventory II. The secondary outcome measures were albumin and triglyceride levels and hematocrit. Results revealed significant between-group differences in physical functional performance and depression but not in albumin level, hematocrit, or triglyceride level. Findings suggest that exercise may play a critical role in physical functional performance and may decrease depression. Exercise should be encouraged and performed during HD in HD centers.


2021 ◽  
Vol 12 (3) ◽  
pp. 296-303
Author(s):  
Alisha-Akbar-Dossa ◽  
Parag-Shrinivas-Ranade ◽  
Rahul-Nagendrasingh-Bisen

Leprosy, also known as Hansen’s disease is caused by Mycobacterium Leprae. Despite being curable, it continues to be a significant health problem in many parts of the world. The prevalence of psychiatric disorders is higher in the leprosy affected population than in the general population. Physical activity has been associated with reduced symptoms of depression and anxiety. It is also associated with improved life satisfaction and psychological well-being. Mental Imagery holds belief as an intervention in the treatment of psychological disorders. This is attributable to its harmless, time and cost effective nature. The study aimed to assess the effect of mental imagery on Depression, Anxiety and Stress in institutionalised Leprosy patients using the DASS-21 (Depression, Anxiety and Stress Scale -21). It was carried out in 34 subjects divided equally in the control and experimental groups. The control group received an aerobic exercise program whereas the experimental group received an aerobic exercise program with mental imagery. It was thus concluded that a statistically significant difference was obtained in the scores of Depression in the experimental group. However, only clinically significant differences could be obtained in the scores of Anxiety and Stress. Thus, mental imagery can be used as an effective adjunct with conventional aerobic exercises for reducing Depression, Anxiety and Stress in institutionalised Leprosy patients.


2016 ◽  
Vol 12 (1) ◽  
pp. 61
Author(s):  
Huldani Huldani

Abstract: Aerobic exercise with mild to moderate intensity will improve health and fitness. Aerobic exercise stimulates an increase in the concentration of IL-8 and will attract neutrophils out of circulation and infiltrate into the damaged tissue for physical activity. To determine the effect of mild and moderate aerobic exercise on levels of interleukin 8 and the number of neutrophils in adolescents, semi-experimental research laboratory with the entire population of male students of SMAN I Banjarbaru. Determination of the sample with inclusion and exclusion criteria obtained 31 samples (9 mild aerobic exercise, moderate aerobic exercise 12 and 10 as a control). The implementation of data collection (blood plasma) immediately after the sample is finished doing aerobic exercise. Blood plasma samples were analyzed by flowcytometri to see the number of neutrophils and elisa to see the levels of Interleukin 8 (IL-8). The results showed that mean plasma levels of IL-8 in mild aerobic group (605.69 ± 123.28) and moderate (718.75 ± 132.55) is lower than the control group (720.80 ± 213.11). Kruskal Wallis statistic test in the three groups no significant difference with p = 0.320. The average number of neutrophils mild aerobic exercise group (52.42 ± 8.29) and moderate (63.60 ± 8.73) was higher than the control group (50.11 ± 5.55), which means that there is an increase in the number of neutrophils after aerobic exercise , with one-way ANOVA statistical test showed different significant with p = 0.001. LSD test found significant differences between mild and moderate aerobic group with p = 0.003, 95% CI. There is no difference between the control group mild aerobic with p = 0.519. There are differences between the groups of moderate aerobic and control with p = 0.000. It can be concluded that mild and moderate aerobic exercise did not affect the increased plasma levels of interleukin-8 in adolescents. Mild aerobic exercise had no effect on the increase in the number of neutrophils in adolescents. Moderate aerobic exercise influence on the increase in the number of neutrophils in adolescents.Keywords :  Aerobic exercise, Interleukin 8, Neutrophyl Abstrak: Latihan aerobik dengan intensitas ringan sampai sedang akan meningkatkan kesehatan dan kebugaran tubuh. Latihan aerobik merangsang peningkatan konsentrasi IL-8 dan akan menarik netrofil dari sirkulasi dan menyusup kejaringan yang rusak karena aktivitas fisik.  Untuk mengetahui pengaruh latihan aerobik ringan dan sedang terhadap kadar interleukin 8 dan jumlah netrofil pada remaja, dilakukan penelitian semi eksperimental laboratorik dengan populasi seluruh pelajar laki-laki SMAN I Banjarbaru. Penentuan besarnya sampel dengan kriteria inklusi dan ekslusi didapatkan 31 sampel (9 latihan aerobik ringan, 12 latihan aerobik sedang, dan 10 sebagai kontrol). Pelaksanaan pengambilan data (plasma darah) segera setelah sampel selesai melakukan latihan aerobik. Plasma darah sampel dianalisa dengan flowcytometri untuk melihat jumlah netrofil dan pemeriksaan elisa untuk melihat kadar Interleukin 8 (IL-8). Hasil penelitian menunjukkan bahwa rerata kadar IL-8 plasma pada kelompok aerobik ringan (605,69 ± 123,28) dan sedang (718,75 ± 132,55) lebih rendah dari kelompok kontrol (720,80 ± 213,11). Secara uji statistik Kruskal Wallis ketiga kelompok tidak ada perbedaan bermakna dengan nilai p = 0,320. Jumlah rerata netrofil kelompok latihan aerobik ringan (52,42 ± 8,29)  dan sedang (63,60 ±  8,73) lebih tinggi daripada kelompok kontrol (50,11 ± 5,55), artinya ada peningkatan jumlah netrofil setelah latihan aerobik. Dengan uji statistik ANOVA satu arah didapatkan hasil yang bermakna berbeda dengan nilai p = 0.001. Uji LSD didapatkan  perbedaan bermakna antara kelompok aerobik ringan dan sedang dengan nilai p = 0,003, IK 95 % . Tidak terdapat perbedaan antara kelompok aerobik ringan dengan kontrol dengan nilai p = 0,519. Terdapat perbedaan antara kelompok aerobik sedang dan kontrol dengan nilai p = 0,000. Dapat disimpulkan bahwa latihan aerobik ringan dan sedang tidak berpengaruh terhadap peningkatan kadar Interleukin-8 plasma pada remaja. Latihan aerobik ringan tidak berpengaruh terhadap peningkatan jumlah netrofil pada remaja. Latihan aerobik sedang berpengaruh terhadap peningkatan jumlah netrofil pada remaja. Kata Kata Kunci : Latihan Aerobik, Interleukin 8, Netrofil


Author(s):  
Vahid Aari Sarraf ◽  
Akram Ameghani ◽  
Alireza Ostadrahymy ◽  
Naser Aghamommadzadeh

Background: Limited energy intake with physical activity is effective to reduce health problems associated with obesity. It has been shown that PYY3-36 level increased after intense exercise. This study investigated effect of exercise therapy on appetite suppression and weight loss among obese and/or overweight women. Methods: There was a quasi-experimental with a pretest-posttest design, two groups (control-exercise) study. Study population was overweight or obese women with 20-34 years old and body mass index 27-34 kg/m2. 13 women were randomly assigned in 2 groups (exercise group with 8 weeks of aerobic exercise training (n=7) and control group (n=6)). Weight, waist circumference, hip circumference, lipid profile, cortisol and PYY3-36 were measured at baseline and end of the study. Results: In baseline of study, there was no significant difference across the groups in factors mean (P>0.05).In control group, after 8 weeks, there was no significant difference in variables.(P>0.05) However, In exercise group significant decrease was observed in BMI, WHR, PYY3-36, TG, CHOLESTROL(TC) and LDL, compared to control group (P<0.05). Conclusion: Aerobic exercise within the 80% of heart rate reserve is beneficial in lipid profile, appetite management and modified anthropometric factors. These studies demonstrate that a cortisol change is not along with PYY3-36 changes. We suggest the longer exercise training for this purpose.


2018 ◽  
Vol 1 (5) ◽  
Author(s):  
Yaya Xiong ◽  
Jia Jia ◽  
Xuanming Hao

Objective In recent years, aerobic exercise has been considered as a method of anti-aging. The aging mechanism in nervous system is closely related to increased activation of neuroglia cells and progressing neuroinflammation with aging.This study tried to shed some light on relationship between chronic inflammation accumulation and aerobic exercise so as to understand how exercise intervened nervous aging through inflammation. In this study, we used D- galactose aging models, applying two intervention ways: aerobic exercise during aging process, after that, to explore the changes of astrocytes, microglia and TNF-α expression. This study aims to provide certain evidence that sport plays a role in anti-aging. Methods Fifty male Sprague-Dawley rats at age of eight weeks, are randomly divided into five groups: control group(A), Aging group(S), Aging while exercising group(YS), After aging Quiet group(SA), After aging exercise group(SY). The aging groups were subcutaneously injected with D- galactose 150mg / kg / d for six weeks. Group YS do swimming exercise three times a week,once 60 minutes in the first six weeks. SY had same exercise intervention in the 7-12 week after injection. We took the hippocampus of rats in A, S, YS at the end of the sixth week, and of those in SA, SY at the end of twelfth week. tested the expression of GFAP, CD11b and TNF-α by immunohistochemical staining. Results 1.GFAP immunohistochemistry: Compared with group A,The expression of GFAP in group S, YS, SA and SY was significantly increased (P<0.01). Compared with group S, The expression of GFAP in group YS significantly decreased by 20.5% (P<0.01). There is no significant difference between group SA and SY(P > 0.05). 2.CD11b immunohistochemistry: Compared with group A, The expression of CD11b in group S, YS, SA and SY was significantly increased (P<0.01). Compared with group S, The expression of CD11b in group YS decreased by 18.4% (P < 0.05).There is no significant difference between group SA and SY(P > 0.05). 3.TNF-α Immunohistochemistry: Compared with group A,The expression of TNF-α in group S, YS, SA and SY was significantly increased (P<0.01). Compared with group S, The expression of TNF-α in group YS decreased by 30.1% (P < 0.01).There is no significant difference between group SA and SY(P > 0.05). Conclusions Exercise could effectively delay aging progression through improving neuroinflammation, but hard to reverse it, so the earlier in age to exercise, the better to delay aging.  


2014 ◽  
Vol 2 (11_suppl3) ◽  
pp. 2325967114S0028
Author(s):  
Şeyda Toprak Çelenay ◽  
Derya Özer Kaya

Objectives: The study aimed to investigate effects of thoracic stabilization exercises on postural back pain, spinal alignment and inclination in university students who had postural back pain. Methods: University students who had minor to moderate postural back pain (18-25 years) were randomly allocated into Exercise (n = 28) and Control (n = 25) Groups. The programs were carried out 8 weeks and 3 days/week. The postural back pain intensity was assessed by Visual Analogue Scale. Spinal alignment and spinal inclination were evaluated with the Spinal Mouse® (Idiag, Fehraltorf, Switzerland). Assessments were applied before (BP) and after program (AP). Wilcoxon and Mann Whitney U tests were utilized. Results: The significant differences were observed for postural back pain (BP: 1.33 (1.16); AP: 0.33 (1.16)), thoracic curvature (BP: 44.50 (11.75); AP: 36.50 (13.25)), lumbar curvature (BP: -29.00 (10.00); AP: -7.00 (19.50)) and spinal inclination (BP: 5.00 (5.25); AP: 0.00 (4.00)) in Exercise Group between BP and AP (p<0.05). No significant differences were observed in Control group for all the parameters (p>0.05). The intergroup comparison showed that postural back pain, thoracic curvature, lumbar curvature and spinal inclination decreased in Exercise Group in comparison to Control Group (p<0.05). Conclusion: The exercise program with eight weeks regular practice was effective on improving postural back pain, spinal alignment and inclination in university students with postural back pain.


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