scholarly journals Effects of different exercise loads on serum betatrophin (ANGPTL-8/lipasin) and cartonectin (CTRP-3) levels in metabolic syndrome

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Muhammed Emre Karaman ◽  
Cengiz Arslan ◽  
Mehmet Ferit Gürsu
Keyword(s):  
Metabolic Syndrome ◽  
Aerobic Exercise ◽  
Tap Water ◽  
Exercise Group ◽  
Anaerobic Exercise ◽  
Male Rats ◽  
Control Group ◽  
Standard Diet ◽  
Positive Effects ◽  
Significant Difference

Abstract Objectives The number of studies examining the circulating level change of betatrophin and cartonectin in metabolic syndrome applying different loads of exercise is limited. The purpose of the present study was to investigate how different loads of exercises regulate the betatrophin and cartonectin levels in metabolic syndrome induced rats. Methods A total of 24 Wistar-Albino male rats were used in the study. Rats were divided into four groups as follows; G1: control group (fed with standard diet and tap water), G2: metabolic syndrome group (without exercise application), G3: metabolic syndrome + aerobic exercise group (aerobic exercise applied), G4: metabolic syndrome + anaerobic exercise group (anaerobic exercise applied). Betatrophin and Cartonectin levels were determined by ELISA method in serum samples. Results There was a statistically significant difference in betatrophin levels between the groups and this differentiation was caused by G2 (p <0.05). Cartonectin levels were not significantly different between groups (p> 0.05). Conclusions It can be concluded that anaerobic exercises have more positive effects on glucose balance in metabolic syndrome than aerobic exercises, and by regulating betatrophin levels, anaerobic exercises indicate this effect.

scholarly journals β-endorphin response to aerobic and anaerobic exercises in Wistar male rats

2020 ◽  
Vol 29 (3) ◽  
pp. 245-9
Author(s):  
Rostika Flora ◽  
Mohammad Zulkarnain ◽  
Sukirno
Keyword(s):  
Physical Exercise ◽  
Aerobic Exercise ◽  
Brain Tissue ◽  
Exercise Group ◽  
Anaerobic Exercise ◽  
Treadmill Running ◽  
Male Rats ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Aerobic And Anaerobic

BACKGROUND Physical exercise is strongly associated with the release of β-endorphin. It is assumed that the type and intensity of physical exercise contributes to the release of β-endorphin. This study aimed to compare levels of β-endorphin in brain tissue in response to aerobic and anaerobic physical exercise. METHODS This study was an experimental laboratory study using 35 male Wistar rats divided into one control group and two physical exercise treatment groups: aerobic and anaerobic. Physical exercise was conducted on an animal treadmill running at aspeed of 20 m/min for 30 min of aerobic exercise and 35 m/min with 1-min intervals every 5 min for 20 min for anaerobic exercises. Each aerobic and anaerobic exercise group was furtherly classified into three subgroups (1×/week, 3×/week, and 7×/week). β-endorphin levels were determined using enzyme-linked immunosorbent assay. The data were analyzed using independent t-test and one-way analysis of variance. RESULTS The highest mean of β-endorphin level was found in the weekly exercise (54.45 [1.41] pg/ml) of aerobic exercise group and daily exercise (70.50 [11.67] pg/ml) of anaerobic exercise group. Mean of β-endorphin level in control group was 33.34 (3.54) pg/ml. A significant increased of β-endorphin mean level (p<0.001) was found in all aerobic and anaerobic exercise groups except the aerobic exercise 7×/week group(37.37 [6.30] pg/ml) compared to control. CONCLUSIONS Both aerobic and anaerobic physical exercise conducted for 6 weeks could increase the level of β-endorphin in brain tissue.

scholarly journals Evaluation of oxidative stress in mice subjected to aerobic exercise

2012 ◽  
Vol 27 (8) ◽  
pp. 544-551 ◽  
Author(s):  
Mônica Cruvinel de Lima ◽  
Guido Marks ◽  
Iandara Schettert Silva ◽  
Baldomero Antonio Kato da Silva ◽  
Lourdes Zélia Zanoni Cônsolo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Smooth Muscle ◽  
Aerobic Exercise ◽  
Striated Muscle ◽  
Exercise Program ◽  
Exercise Group ◽  
Control Group ◽  
Adaptation Period ◽  
Intergroup Comparison ◽  
Significant Difference

PURPOSE: To evaluate the influence of aerobic exercise on oxidative stress in mice. METHODS: The study included twenty female mice Mus musculus-Swiss divided into two groups: sedentary control (GA) and exercise (GB), each containing ten animals. All animals underwent an adaptation period of seven days isolated in individual boxes. After this period, the animals in the exercise group (GB) were trained in angled running wheel with circumference of 25 cm assembled on an articulated axle during five minutes for three consecutive days. On the fourth day, they underwent an exercise program of one session lasting 45 minutes. The evaluation of oxidative stress was performed by determining the levels of malondialhyde derived of lipid peroxidation by the TBA method. The samples were read in a spectrophotometer at 535 nm. RESULTS: No significant difference was observed in the intergroup comparison of MDA levels in the tissues evaluated. A significant difference was observed in the intragroup comparison of MDA levels in the control group (p = 0.0201).The Tukeys' post hoc test indicated significantly lower values of MDA in the smooth muscle in relation to plasma. In the analysis of variance in the exercise group, a significant difference between tissues (p = 0.0009), with significantly lower values in the smooth muscle in relation to plasma (p<0.001) and higher in striated muscle in relation to smooth muscle (p<0.05) was observed. CONCLUSION: There was no change in the analysis of oxidative stress in mice which were undergone a single session of aerobic exercise.

scholarly journals Effects of equine-assisted activities on attention and quality of life in children with cerebral palsy in a randomized trial: examining the comorbidity with attention-deficit/hyperactivity disorder

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Bomi Ahn ◽  
Yoo-Sook Joung ◽  
Jeong-Yi Kwon ◽  
Dong Ik Lee ◽  
Soohwan Oh ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Cerebral Palsy ◽  
Exercise Group ◽  
Control Group ◽  
Commission Error ◽  
Positive Effects ◽  
Significant Difference ◽  
Children With Cerebral Palsy ◽  
Equine Assisted

Abstract Background Attention problems and decreased quality of life are frequently accompanied in Cerebral Palsy (CP), which can negatively affect rehabilitation of physical disability. However, the majority of affected children remain untreated in the aspects of attention or psychosocial factors. Equine-Assisted Activities and Therapies (EAAT) use horse as a therapeutic modality including grooming as well as mounted riding activities in which patients exercise and experience mounted stimulation. It is known to help improve attention in children with ADHD, so that it can be an exercise therapy that is expected to improvement of attention as well as rehabilitating effects in CP patients. EAA may be a promising strategy to address the unmet need for CP patients. This study aims to investigate the efficacy of EAA for children with CP, those with both CP and ADHD and confirm the comorbidity between CP and ADHD. Methods Forty-six children with cerebral palsy participated in this study. For the exercise group, they participated in a 40-min session twice a week for a 16-week period, while the control group engaged in daily life without any special treatments. Each children individually were assessed on attention and psychological wellbeing at baseline and post-treatment. Comorbidity were identified based on the Diagnostic and Statistical Manual of Mental Disorder 5th edition (DSM-5) and confirmed by Korean Kiddie-Schedule for Affective Disorders and Schizophrenia Present and Lifetime Version (K-SADS-PL). Results Perseveration rated using the Conner’s Performance Test (CPT) showed a significant decrease only in the exercise group (p < .024). However, no significant improvement in children’s quality of life was observed after EAA program compared with control group. Among the total participants, fifteen children (31.91%) were diagnosed with ADHD. When conducting an additional analysis with the subsample of CP patients diagnosed with ADHD, the d’, commission error and perseveration showed a significant decrease only in the exercise group. Children with CP and ADHD reported an improvement in quality of life both in exercise and control group, but only in the exercise group social functioning exhibited a significant difference. Conclusion The positive effects of the EAA on attention and quality of life were confirmed. Children with CP in the exercise group were more capable to sustain their attention longer. Those with CP and ADHD showed an increase in attention and perceived to have better social skills after receiving 16 weeks of EAA compared to those in the control group. Considering high comorbidity of CP and ADHD, it seems that the EAA program could be the better alternative treatment for CP with attentional problem. The results of this study will contribute to growing evidence for the efficacy of EAA in children especially with CP and ADHD. Trial registration This trial was registered on ClinicalTrials.gov (NCT03870893). Registered 26 July 2017.

scholarly journals Pengaruh Latihan Aerobik Ringan dan Sedang terhadap Kadar Interleukin 8 dan Jumlah Netrofil pada Remaja

2016 ◽  
Vol 12 (1) ◽  
pp. 61
Author(s):  
Huldani Huldani
Keyword(s):  
Blood Plasma ◽  
Aerobic Exercise ◽  
Plasma Levels ◽  
Exercise Group ◽  
Interleukin 8 ◽  
Male Students ◽  
Moderate Intensity ◽  
Control Group ◽  
Significant Difference ◽  
And Control

Abstract: Aerobic exercise with mild to moderate intensity will improve health and fitness. Aerobic exercise stimulates an increase in the concentration of IL-8 and will attract neutrophils out of circulation and infiltrate into the damaged tissue for physical activity. To determine the effect of mild and moderate aerobic exercise on levels of interleukin 8 and the number of neutrophils in adolescents, semi-experimental research laboratory with the entire population of male students of SMAN I Banjarbaru. Determination of the sample with inclusion and exclusion criteria obtained 31 samples (9 mild aerobic exercise, moderate aerobic exercise 12 and 10 as a control). The implementation of data collection (blood plasma) immediately after the sample is finished doing aerobic exercise. Blood plasma samples were analyzed by flowcytometri to see the number of neutrophils and elisa to see the levels of Interleukin 8 (IL-8). The results showed that mean plasma levels of IL-8 in mild aerobic group (605.69 ± 123.28) and moderate (718.75 ± 132.55) is lower than the control group (720.80 ± 213.11). Kruskal Wallis statistic test in the three groups no significant difference with p = 0.320. The average number of neutrophils mild aerobic exercise group (52.42 ± 8.29) and moderate (63.60 ± 8.73) was higher than the control group (50.11 ± 5.55), which means that there is an increase in the number of neutrophils after aerobic exercise , with one-way ANOVA statistical test showed different significant with p = 0.001. LSD test found significant differences between mild and moderate aerobic group with p = 0.003, 95% CI. There is no difference between the control group mild aerobic with p = 0.519. There are differences between the groups of moderate aerobic and control with p = 0.000. It can be concluded that mild and moderate aerobic exercise did not affect the increased plasma levels of interleukin-8 in adolescents. Mild aerobic exercise had no effect on the increase in the number of neutrophils in adolescents. Moderate aerobic exercise influence on the increase in the number of neutrophils in adolescents.Keywords :  Aerobic exercise, Interleukin 8, Neutrophyl Abstrak: Latihan aerobik dengan intensitas ringan sampai sedang akan meningkatkan kesehatan dan kebugaran tubuh. Latihan aerobik merangsang peningkatan konsentrasi IL-8 dan akan menarik netrofil dari sirkulasi dan menyusup kejaringan yang rusak karena aktivitas fisik.  Untuk mengetahui pengaruh latihan aerobik ringan dan sedang terhadap kadar interleukin 8 dan jumlah netrofil pada remaja, dilakukan penelitian semi eksperimental laboratorik dengan populasi seluruh pelajar laki-laki SMAN I Banjarbaru. Penentuan besarnya sampel dengan kriteria inklusi dan ekslusi didapatkan 31 sampel (9 latihan aerobik ringan, 12 latihan aerobik sedang, dan 10 sebagai kontrol). Pelaksanaan pengambilan data (plasma darah) segera setelah sampel selesai melakukan latihan aerobik. Plasma darah sampel dianalisa dengan flowcytometri untuk melihat jumlah netrofil dan pemeriksaan elisa untuk melihat kadar Interleukin 8 (IL-8). Hasil penelitian menunjukkan bahwa rerata kadar IL-8 plasma pada kelompok aerobik ringan (605,69 ± 123,28) dan sedang (718,75 ± 132,55) lebih rendah dari kelompok kontrol (720,80 ± 213,11). Secara uji statistik Kruskal Wallis ketiga kelompok tidak ada perbedaan bermakna dengan nilai p = 0,320. Jumlah rerata netrofil kelompok latihan aerobik ringan (52,42 ± 8,29)  dan sedang (63,60 ±  8,73) lebih tinggi daripada kelompok kontrol (50,11 ± 5,55), artinya ada peningkatan jumlah netrofil setelah latihan aerobik. Dengan uji statistik ANOVA satu arah didapatkan hasil yang bermakna berbeda dengan nilai p = 0.001. Uji LSD didapatkan  perbedaan bermakna antara kelompok aerobik ringan dan sedang dengan nilai p = 0,003, IK 95 % . Tidak terdapat perbedaan antara kelompok aerobik ringan dengan kontrol dengan nilai p = 0,519. Terdapat perbedaan antara kelompok aerobik sedang dan kontrol dengan nilai p = 0,000. Dapat disimpulkan bahwa latihan aerobik ringan dan sedang tidak berpengaruh terhadap peningkatan kadar Interleukin-8 plasma pada remaja. Latihan aerobik ringan tidak berpengaruh terhadap peningkatan jumlah netrofil pada remaja. Latihan aerobik sedang berpengaruh terhadap peningkatan jumlah netrofil pada remaja. Kata Kata Kunci : Latihan Aerobik, Interleukin 8, Netrofil

Natural mineral-rich water ingestion improves hepatic and fat glucocorticoid-signaling and increases sirtuin 1 in an animal model of metabolic syndrome

2015 ◽  
Vol 21 (2) ◽  
Author(s):  
Cidália D. Pereira ◽  
Milton Severo ◽  
Delminda Neves ◽  
António Ascensão ◽  
José Magalhães ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Animal Model ◽  
Water Intake ◽  
Tap Water ◽  
Plasma Corticosterone ◽  
Sirtuin 1 ◽  
Male Rats ◽  
Standard Diet ◽  
Natural Mineral ◽  
Glucocorticoid Signaling

AbstractWe recently reported protection against metabolic syndrome (MetSyn) induction and endothelial dysfunction by natural mineral-rich water intake in fructose-fed Sprague-Dawley rats. As glucocorticoids are critical to MetSyn development, we aimed to further characterize the beneficial effects of mineral-rich water intake in that animal model, by assessing relevant effectors in glucocorticoid-signaling in liver and subcutaneous (SCAT) and visceral (VAT) adipose tissues, sites with a central role in metabolic (dys)regulation.Adult male rats had free access to standard diet and different drinking solutions (8 weeks): a) tap water (CONT), b) 10% fructose/tap water (FRUCT) or c) 10% fructose/mineral-rich water (FRUCTMIN). 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1), glucocorticoid receptor (GR), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1-α) and sirtuin 1 (Sirt1) tissue protein expressions were evaluated by Western blot. Plasma corticosterone (ELISA) and non-esterified fatty acids (NEFA) levels were quantified spectrophotometrically.Expectedly, Sirt1 and PGC1-α significantly decreased in liver, 11β-HSD1 tended to increase in VAT and tended to decrease in liver and SCAT, and plasma corticosterone tended to increase in FRUCT vs. CONT. Mineral-rich water showed a trend towards a reduction of these fructose effects and significantly increased hepatic Sirt1 vs. CONT and FRUCT. GR significantly increased in VAT and plasma NEFA strongly tended to increase in FRUCTMIN vs. CONT and FRUCT.Glucocorticoid-signaling was different among SCAT and VAT and also in liver. Mineral-rich water modulation of fructose effects on glucocorticoid-signaling and Sirt1 underlines the better metabolic profile found earlier.

scholarly journals Comparison of Nitric Oxide Changes in Hippocampal Tissue and Pain Sensation in Male Rats Parkinson's Model Following 6 Weeks of Continuous and Intermittent Training

2020 ◽  
Author(s):  
Saeid Moradi ◽  
Abdol Hamid Habibi ◽  
Mohammad Reza Tabande ◽  
Saeid Shakerian
Keyword(s):  
Nitric Oxide ◽  
Aerobic Exercise ◽  
Interval Training ◽  
Male Rats ◽  
Control Group ◽  
Pain Sensation ◽  
Interval Group ◽  
Model Following ◽  
Hippocampal Tissue ◽  
Significant Difference

Introduction: Despite the beneficial roles of nitric oxide, its excessive synthesis causes neurodegenerative diseases. Therefore, the aim of the present study was to compare the nitric oxide changes in the hippocampal tissue and the pain sensation of male Parkinson's rats after 6 weeks of continuous and interval training. Methods: In this experimental study, 24 Wistar male rats were used. Rats were treated with Parkinson's using reserpine, and then divided into four groups (n=6). Aerobic exercise was performed for 6 weeks and 5 sessions per week. The duration of training increased from the first to the sixth week, it increased from 15 minutes on the first day to 40 minutes in the sixth week. The interval group performed the specified duration of the exercise twice in the first week, four times in the second to fourth weeks, and six times in the fifth to sixth weeks. At the end of 6 weeks, pain sensation was measured using formalin test and then rats were sacrificed. The tissue of the hippocampus was separated, and nitric oxide was measured using the ELISA method and special kits. The results were analyzed using one-way analysis of variance and LSD follow-up. Results:The results showed that Parkinson's disease increased nitric oxide levels (p ≤0.05). Continuous and interval aerobic exercise reduced nitric oxide (p ≤0.05). There was no significant difference between the two training groups (p ≤0.68). In addition, Parkinson's control group showed a significant increase in pain compared to the control group (p ≤0.01). Two types of exercise reduced pain (p ≤0.05), but there was no significant difference between the two groups (p≤0.23). Conclusion:According to the findings of this study, continuous and periodic aerobic exercise reduced nitric oxide levels and pain in the rats of the Parkinson's model.

scholarly journals PENGARUH PEMBERIAN CUKA APEL (Malus sylvestris Mill) VARIETAS MANALAGI TERHADAP KADAR TRIGLISERIDA DARAH TIKUS PUTIH (Rattus norvegicus) JANTAN GALUR WISTAR YANG DIBERI DIET TINGGI LEMAK DAN DIINDUKSI ALOKSAN

2019 ◽  
Vol 20 (2) ◽  
pp. 70-75
Author(s):  
Yuliyanti Yuliyanti ◽  
A. Sareh Arjono Tjandra ◽  
Herin Setianingsih
Keyword(s):  
Rattus Norvegicus ◽  
High Fat Diet ◽  
White Male ◽  
Male Rats ◽  
Control Group ◽  
Standard Diet ◽  
High Fat ◽  
Malus Sylvestris ◽  
Wistar Strain ◽  
Significant Difference

Hypertriglyceridemia is one of the risk factors for cardiovascular disease and acute pancreatitis (Pejic and Lee, 2006; Brahm and Hegele, 2013; Afari et al., 2015). One way to treat it by using the apple vinegar (Malus sylvestris Mill) of manalagi varieties which contained high antioxidants and acetic acid as well as more durable than the fresh fruit. The study aimed to know the effect of the Apple vinegar of manalagi varieties (Malus sylvestris Mill) to blood triglyceride levels of white male rats (Rattus norvegicus) wistar strain with high-fat diet and induced by alloxan. The study used the post test only control group design, with 24 rats samples which were divided into three groups. The negative control group (K0) only received standard diet for 24 days, the positive control group (K1) received a high-fat diet for 7 days and induced by alloxan with doses of 90 mg/kgBW of rats, and treatment group (K2) was given a high-fat diet for 7 days, induced by alloxan and received the apple vinegar (Malus sylvestris Mill) of manalagi varieties with a dose of 0,27 ml/100 g BW of rats for 14 days. The results by using one way ANOVA showed the significance value of the data p = 0,974 (p>0,05) so that there is no significant difference of the mean of blood triglycerides levels between K0, K1 and K2. This study concluded that apple vinegar (Malus sylvestris Mill) of manalagi varieties can not significantly lower the blood triglyceride levels of white male rats (Rattus norvegicus) wistar strain with high-fat diet and induced by alloxan. Further study about apple vinegar of manalagi varieties is required to define its effects.  Keywords—: High-Fat Diet, Alloxan, Triglyceride, Apple Vinegar (Malus sylvestris Mill) of Manalagi Varieties

scholarly journals Effect of 8-week aerobic training on lipid profile, PYY3-36, and cortisol levels among obese women

2020 ◽  
Vol 42 (5) ◽  
pp. 511-519
Author(s):  
Vahid Aari Sarraf ◽  
Akram Ameghani ◽  
Alireza Ostadrahymy ◽  
Naser Aghamommadzadeh
Keyword(s):  
Exercise Training ◽  
Aerobic Exercise ◽  
Lipid Profile ◽  
Exercise Group ◽  
Aerobic Exercise Training ◽  
Control Group ◽  
Obese Women ◽  
Study Results ◽  
Significant Difference ◽  
Quasi Experimental

Background: Limited energy intake with physical activity is effective to reduce health problems associated with obesity. It has been shown that PYY3-36 level increased after intense exercise. This study investigated effect of exercise therapy on appetite suppression and weight loss among obese and/or overweight women. Methods: There was a quasi-experimental with a pretest-posttest design, two groups (control-exercise) study. Study population was overweight or obese women with 20-34 years old and body mass index 27-34 kg/m2. 13 women were randomly assigned in 2 groups (exercise group with 8 weeks of aerobic exercise training (n=7) and control group (n=6)). Weight, waist circumference, hip circumference, lipid profile, cortisol and PYY3-36 were measured at baseline and end of the study. Results: In baseline of study, there was no significant difference across the groups in factors mean (P>0.05).In control group, after 8 weeks, there was no significant difference in variables.(P>0.05) However, In exercise group significant decrease was observed in BMI, WHR, PYY3-36, TG, CHOLESTROL(TC) and LDL, compared to control group (P<0.05). Conclusion: Aerobic exercise within the 80% of heart rate reserve is beneficial in lipid profile, appetite management and modified anthropometric factors. These studies demonstrate that a cortisol change is not along with PYY3-36 changes. We suggest the longer exercise training for this purpose.

scholarly journals Hurdle Aerobic Exercise Increases Angiogenesis and Neuroplasticity in the Hippocampus and Improves the Spatial Memory Ability of Middle-aged Mice

2020 ◽  
Vol 8 (A) ◽  
pp. 395-402
Author(s):  
Irfannuddin Irfannuddin ◽  
Minarma Siagian ◽  
Sri Jusman ◽  
Jan Purba ◽  
Ermita Ilyas ◽  
...  
Keyword(s):  
Cognitive Function ◽  
Spatial Memory ◽  
Aerobic Exercise ◽  
Blood Vessels ◽  
Morris Water Maze Test ◽  
Control Group ◽  
Memory Ability ◽  
Positive Effects ◽  
Significant Difference ◽  
The Brain

BACKGROUND: Complex aerobic exercise is believed to induce positive effects on neuron structure and cognitive function. Long-term and continual cognitive stimulation increases neuroplasticity by stimulating the synthesis of neuronal growth proteins and the formation of new synapses. Exercise also increases the ability of neurons to survive and improves brain vascularization. Further investigations should be conducted to explore what types of aerobic exercise are beneficial for cognitive function. AIM: This study investigated the effects of hurdle aerobic exercise on developmentally regulated brain protein-A (Drebrin-A) as a neuroplasticity indicator, and on vascular endothelial growth factor (VEGF) as an angiogenesis marker in the hippocampus. METHODS: This study was an experimental study with post-test only control group design. Thirty-three adult mice were divided into control, hurdle aerobic runner (HAR), and plain aerobic runner (PAR) groups (n = 11 for each group). Fiberglass running wheels were originally designed and modified to assemble hurdles inside with adjustable speed. Speed adaptation was intended to achieve aerobic intensity. The experiment was performed 5 times a week for 8 weeks. The Morris water maze test (MWMT) was used to assess spatial memory ability. One day after the last running exercise and final MWMT, the mice were sacrificed and the right side of the hippocampus was obtained for Drebrin-A analysis by enzyme-linked immunosorbent assay (ELISA). The entire right side brain tissue after hippocampus was removed then used for the neuroglobin ELISA assay. To analyze VEGF expression and calculation of blood vessel, the left side of the brain was prepared for hematoxylin eosin and immunohistochemistry staining. To assess the effect of exercise on vascular widening, the analysis of the slides was performed by calculating the percentage of blood vessels with diameters more than 15 μm. One-way ANOVA and Fisher’s least significant difference test was used for statistical analysis. RESULTS: There was a significant difference in the levels of Drebrin-A between the HAR and PAR groups. Both exercise groups had higher levels of Drebrin-A than the control group. HAR and PAR groups exhibited significantly higher percentages of blood vessels expressing VEGF in hippocampus compared to control. HAR and PAR groups had the higher percentages of larger vessels compare to control. There was no significant difference of neuroglobin levels among the three groups. Both the HAR and PAR groups exhibited better spatial memory than the control group. CONCLUSION: Both aerobic exercises induced positive effects on brain angiogenesis, while the intensity of aerobic exercises did not result in high hypoxic stress in the brain.

scholarly journals PO-241 Effects of Aerobic Exercise on Activation of Neuroglia Cell and Expression of Inflammatory Factors in Aging Rats

2018 ◽  
Vol 1 (5) ◽  
Author(s):  
Yaya Xiong ◽  
Jia Jia ◽  
Xuanming Hao
Keyword(s):  
Aerobic Exercise ◽  
Exercise Intervention ◽  
Exercise Group ◽  
Inflammatory Factors ◽  
Control Group ◽  
Sprague Dawley ◽  
Tnf Α ◽  
Significant Difference ◽  
Group A ◽  
Aging Models

Objective In recent years, aerobic exercise has been considered as a method of anti-aging. The aging mechanism in nervous system is closely related to increased activation of neuroglia cells and progressing neuroinflammation with aging.This study tried to shed some light on relationship between chronic inflammation accumulation and aerobic exercise so as to understand how exercise intervened nervous aging through inflammation. In this study, we used D- galactose aging models, applying two intervention ways: aerobic exercise during aging process, after that, to explore the changes of astrocytes, microglia and TNF-α expression. This study aims to provide certain evidence that sport plays a role in anti-aging. Methods Fifty male Sprague-Dawley rats at age of eight weeks, are randomly divided into five groups: control group(A), Aging group(S), Aging while exercising group(YS), After aging Quiet group(SA), After aging exercise group(SY). The aging groups were subcutaneously injected with D- galactose 150mg / kg / d for six weeks. Group YS do swimming exercise three times a week,once 60 minutes in the first six weeks. SY had same exercise intervention in the 7-12 week after injection. We took the hippocampus of rats in A, S, YS at the end of the sixth week, and of those in SA, SY at the end of twelfth week. tested the expression of GFAP, CD11b and TNF-α by immunohistochemical staining. Results 1.GFAP immunohistochemistry: Compared with group A,The expression of GFAP in group S, YS, SA and SY was significantly increased (P<0.01). Compared with group S, The expression of GFAP in group YS significantly decreased by 20.5% (P<0.01). There is no significant difference between group SA and SY(P > 0.05). 2.CD11b immunohistochemistry: Compared with group A, The expression of CD11b in group S, YS, SA and SY was significantly increased (P<0.01). Compared with group S, The expression of CD11b in group YS decreased by 18.4% (P < 0.05).There is no significant difference between group SA and SY(P > 0.05). 3.TNF-α Immunohistochemistry: Compared with group A,The expression of TNF-α in group S, YS, SA and SY was significantly increased (P<0.01). Compared with group S, The expression of TNF-α in group YS decreased by 30.1% (P < 0.01).There is no significant difference between group SA and SY(P > 0.05). Conclusions Exercise could effectively delay aging progression through improving neuroinflammation, but hard to reverse it, so the earlier in age to exercise, the better to delay aging.  

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