scholarly journals Cytotoxicity of separation orthodontic elastics

2012 ◽  
Vol 17 (4) ◽  
pp. 110-114
Author(s):  
Rogério Lacerda dos Santos ◽  
Matheus Melo Pithon ◽  
Fernanda Otaviano Martins ◽  
Maria Teresa Villela Romanos
Keyword(s):  
Culture Medium ◽  
Blue Color ◽  
Dye Uptake ◽  
Time Intervals ◽  
Cytotoxic Effects ◽  
Green Color ◽  
Significant Difference ◽  
Group A ◽  
Uptake Test ◽  
Human Laryngeal Carcinoma

OBJECTIVE: To test the hypothesis that there is no difference in cytotoxicity between separating elastics of different manufacturers. METHODS: The present article compared latex elastics (4.0 mm, 4.4 mm and 4.8 mm) of four different manufacturers. The sample was allocated to seven groups of 9 elastics: Group A (American Orthodontics, green color, modules), Groups M1 and M2 (Morelli, blue color, modules and free in pack respectively), Groups M3 and M4 (Morelli, green color, modules and free in pack respectively), Group U (Uniden, blue color, free in pack) and Group T (Tecnident, blue color, free in pack) regarding their possible cytotoxic effects on oral tissues. Cytotoxicity assays were performed using cell culture medium containing epithelioid-type cells (Hep-2 line) derived from human laryngeal carcinoma and submitted to the methods for evaluating the cytotoxicity by the "dye-uptake" test, at time intervals 24, 48, 72 and 168 h. Data were compared by analysis of variance (ANOVA) and Tukey's test (p < 0.05). RESULTS: Results showed statistically significant difference (p < 0.05) between group U and all the other Groups (A, M1, M2, M3, M 4 and T) at 24 and 48 hours. CONCLUSIONS: Uniden elastics evoked more cell lysis at 24 and 48 h, although, all brands showed biocompatibility from 72 h onwards.

Embryo sHLA-G secretion is related to pregnancy rate

Zygote ◽  
2019 ◽  
Vol 27 (02) ◽  
pp. 78-81
Author(s):  
R. Rodríguez Díaz ◽  
R. Blanes Zamora ◽  
R. Vaca Sánchez ◽  
J. González Pérez ◽  
J.C. Alberto Bethencourt
Keyword(s):  
Pregnancy Rate ◽  
Culture Medium ◽  
Embryo Quality ◽  
Statistical Significance ◽  
Preimplantation Embryos ◽  
Enzyme Linked Immunosorbent Assay ◽  
Significant Difference ◽  
Invasive Method ◽  
Group A

SummaryHLA-G expression has been detected in early preimplantation embryos and it has been postulated that a relationship between embryonic expression of this factor and successful pregnancy may exist. Forty-six patients were prospectively selected from our centre ‘Unidad de Reproducción Humana, Hospital Universitario de Canarias’ for conducting this study. In all cases, metaphase II (MII) oocytes were fertilized using intracytoplasmic sperm injection 2–4 h after retrieval. Embryos were cultured individually in 20 µl droplets of G-1 medium (VitroLife) under oil at 37°C and a 6% CO2environment. Fertilization was assessed at 18 h postinsemination and all oocytes fertilized were passed into a new culture plaque individually in 300 µl culture medium until day 3 of culture. The culture medium was examined for the expression and secretion of sHLA-G with a sandwich enzyme-linked immunosorbent assay kit (BioVendor, Heidelberg, Germany) according to the manufacturer’s instructions. We found statistical significance between higher levels of sHLA-G secretion and pregnancy rate. When both groups were compared there was no difference in embryo quality of transferred embryos, but a significant difference in the number of oocytes and the embryo quality of the cohort existed that was greater in the pregnant group. A standardized sHLA-G assay with a specifically defined range and standard units provides a non-invasive method to identify the most competent embryos for transfer.

scholarly journals Cytotoxic effects of White-MTA and MTA-Bio cements on odontoblast-like cells (MDPC-23)

2010 ◽  
Vol 21 (1) ◽  
pp. 24-31 ◽  
Author(s):  
Fernanda Campos Rosetti Lessa ◽  
Andreza Maria Fábio Aranha ◽  
Josimeri Hebling ◽  
Carlos Alberto de Souza Costa
Keyword(s):  
Culture Medium ◽  
Cell Metabolism ◽  
Mineral Trioxide Aggregate ◽  
Negative Control ◽  
Control Group ◽  
Cytotoxic Effects ◽  
Significant Difference ◽  
Cell Organization ◽  
New Formulation ◽  
And Control

This study evaluated the cytotoxic effects of 2 mineral trioxide aggregate (MTA) cements - White-MTA-Angelus and a new formulation, MTA-Bio - on odontoblast-like cell (MDPC-23) cultures. Twenty-four disc-shaped (2 mm diameter x 2 mm thick) specimens were fabricated from each material and immersed individually in wells containing 1 mL of DMEM culture medium for either 24 h or 7 days to obtain extracts, giving rise to 4 groups of 12 specimens each: G1 - White-MTA/24 h; G2 - White-MTA/7 days; G3 - MTA-Bio/24 h; and G4 - MTA-Bio/7 days. Plain culture medium (DMEM) was used as a negative control (G5). Cells at 30,000 cells/cm² concentration were seeded in the wells of 24-well plates and incubated in a humidified incubator with 5% CO2 and 95% air at 37ºC for 72 h. After this period, the culture medium of each well was replaced by 1 mL of extract (or plain DMEM in the control group) and the cells were incubated for additional 2 h. Cell metabolism was evaluated by the MTT assay and the data were analyzed statistically by ANOVA and Tukey's test (α=0.05). Cell morphology and the surface of representative MTA specimens of each group were examined by scanning electron microscopy. There was no statistically significant difference (p>0.05) between G1 and G2 or between G3 and G4. No significant difference (p>0.05) was found between the experimental and control groups either. Similar cell organization and morphology were observed in all groups, regardless of the storage periods. However, the number of cells observed in the experimental groups decreased compared to the control group. MTA-Bio presented irregular surface with more porosities than White-MTA. In conclusion, White-MTA and MTA-Bio presented low cytotoxic effects on odontoblast-like cell (MDPC-23) cultures.

scholarly journals Evaluation of the Efficacy of Mineralized Dentin Graft in the Treatment of Intraosseous Defects: An Experimental In Vivo Study

Medicina ◽  
2022 ◽  
Vol 58 (1) ◽  
pp. 103
Author(s):  
Nuray Özkahraman ◽  
Nilüfer Bölükbaşı Balcıoğlu ◽  
Merva Soluk Tekkesin ◽  
Yusuf Altundağ ◽  
Serdar Yalçın
Keyword(s):  
Bone Defects ◽  
The Other ◽  
Time Intervals ◽  
Autogenous Graft ◽  
Significant Difference ◽  
Different Types ◽  
Group A ◽  
Materials Used ◽  
Group D

Background and Objectives: Dentin grafts have osteoinductive and osteoconductive properties and are considered as an alternative to autogenous graft. This study evaluates the efficacy of autogenous mineralized dentin graft (AMDG) alone or with xenograft and compares it with those of various graft materials used in the treatment of intraosseous bone defects. Materials and Methods: The third incisor teeth of six sheep (2–3 years old) were extracted and AMDG was obtained. Six defects were prepared on each tibia of these six sheep: empty defect (group E); autogenous graft (group A), dentin graft (group D), xenograft (group X), autogenous + xenograft (group A + X) and dentin + xenograft (group D + X). Three sheep in each group were sacrificed in the post-operative 3rd and 6th week and the histologic analyses were performed. Results: The D and D + X groups showed histological features similar to the other groups in the 3rd and 6th weeks. No statistically significant difference was found regarding the rates of new bone formation between the D and D + X groups (p = 1.0) and the other groups at both time intervals (p > 0.05). Conclusions: Similar results observed in this study between groups A, D, X, A + X and D + X demonstrate that AMDG can be successfully used in the treatment of intraosseous bone defects. Further experimental and clinical studies are needed to be able to evaluate the effectiveness of dentin grafts in different types of indications.

SP1.2.5Robot-assisted Colorectal Surgery: Short-term Outcome of First 70 cases at Different Time-intervals along the Learning Curve

2021 ◽  
Vol 108 (Supplement_7) ◽  
Author(s):  
Sandeep Singh ◽  
William Rea ◽  
Shaun Nai ◽  
Tamas Sollei ◽  
Neil Kukreja ◽  
...  
Keyword(s):  
Colorectal Surgery ◽  
Learning Curve ◽  
Operating Time ◽  
Total Duration ◽  
Time Intervals ◽  
Term Outcome ◽  
Female Ratio ◽  
Significant Difference ◽  
Group A ◽  
Group B

Abstract Aim To analyse the evolution of robot-assisted colorectal surgery (RACS). Methods All the patients who underwent RACS between June 2018 and December 2020 were selected. Study period was divided into 3 intervals along the learning curve; group A represents the 1st 10 months, group B 2nd 10 and group C last 11. Results Over the 31-month study period, 70 RACS were performed. Male to female ratio was 4:3, with mean age of 66 years and BMI 28.7. ASA score was reported as 1-2 in 65% patients and 35% as 3. Indication was malignancy for 89% patients and the remainder benign pathology. High or low anterior resection performed were 72%, right hemicolectomies 21%, Hartmann’s 3%, abdominoperineal resections 3%, and left hemicolectomy 1%. RACS performed in group A was 20 compared to 19 in B and 31 C. Type of RACS performed, BMI, ASA, complications, anastomotic leaks, R0 resections and harvested lymph nodes were unrelated to selected time-intervals along learning curve. Mean total duration of procedure dropped down to 247 minutes in group C from 375 in group A (p = &lt;0.001). No significant difference in mean length of stay and readmission rate was observed in 3 groups (p = 0.7, p = 0.59). Conclusion The study demonstrates that introduction of this new surgical technique is safe even in the early phase of adoption with no significant difference in pre-and post-operative morbidities. Significant difference in time taken for surgery was observed with reduction of 88 minutes in average operating time between the start and end of the study.

scholarly journals Comparative Evaluation of Triphala and Ela Decoction With 0.2% Chlorhexidine as Mouthwash in the Treatment of Plaque-Induced Gingivitis and Halitosis: A Randomized Controlled Clinical Trial

2016 ◽  
Vol 22 (3) ◽  
pp. 468-472 ◽  
Author(s):  
Pratibha Mamgain ◽  
Abhishek Kandwal ◽  
Ravindra K. Mamgain
Keyword(s):  
Group Analysis ◽  
T Test ◽  
Plaque Index ◽  
Controlled Clinical Trial ◽  
Base Line ◽  
Time Intervals ◽  
Intergroup Comparison ◽  
Significant Difference ◽  
Group A ◽  
Group B

Present study evaluates efficacy of Trifala and Ela as plaque controlling agent and compares it with chlorhexidine. Aim. To evaluate Antigingivitis, Antiplaque and Antihalitosis effect of Triphala and Ela decoction. A randomized sample of 60 patients with plaque induced gingivitis was enrolled and equally divided into two groups group A and group B. Group A was given Trifala and Ela decoction and Group B Chlorehexidine mouthwash for 21 days twice daily. Gingival inflammation index, plaque index and Organoleptic scoring scale was recorded at baseline, 14th day and 21st day. Comparing the plaque index for Group A with group B the reduction in from baseline to 14 day was 42.59 % and 38.62% respectively while from baseline to 21 day was 56.20% and 68.57% respectively. On comparing Gingival index for group A with group B the reduction from baseline to 14 day was 31.95% and 38.62 % respectively while from baseline to 21 day was 69.95 % and 68.57% respectively. Halitosis Percentage reduction at 14th day from base line was 33.33% and 38.18%; at 21 day from baseline 66.66% and 72.72% respectively for group A and group B. No statistical significant difference for intergroup comparison was found using paired t test. Intra group analysis using unpaired t test was significant for all the indices at different time intervals. Triphala and Ela decoction is organic, easy to prepare economical and equally effective as compared to chlorhexidine mouthwash.

scholarly journals Osteogenesis of osteogenic matrix cell sheets preserved in culture medium in a rat model

2018 ◽  
Vol 27 (8) ◽  
pp. 1281-1288
Author(s):  
Tsutomu Kira ◽  
Manabu Akahane ◽  
Noriko Ouji-Sageshima ◽  
Takamasa Shimizu ◽  
Tadanobu Onishi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Culture Medium ◽  
Stem Cell Markers ◽  
Cell Sheets ◽  
Cytotoxic Effects ◽  
Matrix Cell ◽  
Significant Difference ◽  
Preservation Conditions ◽  
Ldh Release ◽  
Stromal Stem Cells

Osteogenic matrix cell sheets (OMCSs) are ideal for bone regeneration. Transportation of OMCSs may be necessary, during which their osteogenic ability must be maintained. Here, we evaluated different media and temperatures for OMCS preservation. Bone marrow stromal/stem cells (BMSCs) were obtained from Fischer rats and analyzed for stem cell markers by flow cytometry. OMCSs were prepared from BMSCs by treatment with dexamethasone and ascorbic acid phosphate. After OMCS collection, they were stored in minimum essential medium (MEM) or Hank’s balanced salt solution (HBSS) at 37, 22, or 4°C for 24 hours. Cell viability and cytotoxic effects in the preservation conditions were determined by adenosine triphosphate (ATP) contents and lactate dehydrogenase (LDH) release, respectively. Osteogenesis was assessed by subcutaneously implanting preserved OMCSs around β-tricalcium phosphate ceramic disks into syngeneic rats. Implants were evaluated by alkaline phosphatase (ALP) activities, osteocalcin contents, and histology. Mesenchymal stem cells comprised 51% of primary cultured BMSCs. ATP contents were significantly different in OMCSs stored in MEM or HBSS at 22°C and 4°C. LDH release was significantly different in OMCSs stored in HBSS at 22°C and 4°C. The highest LDH release was observed in OMCSs stored in HBSS at 37°C. ALP activities and osteocalcin contents were the lowest in implanted OMCSs stored in HBSS at 37°C at four weeks after subcutaneous implantation. There was a significant difference in the osteocalcin levels of implanted OMCSs stored in MEM at 37°C and HBSS at 4°C. Abundant bone tissue around and inside disks was found in histological sections of OMCSs stored in all preservation conditions except for MEM and HBSS at 37°C. Maintaining the osteogenic ability of OMCSs during transport is important, and preservation of OMCSs in MEM or HBSS at 4°C or 22°C is a simple and inexpensive method.

Correlation Of Haematological Parameters In Deep Vein Thrombosis

1981 ◽  
Author(s):  
D R Donaldson ◽  
M J Crow ◽  
G A Davies ◽  
S M Rajah ◽  
D A Watson
Keyword(s):  
Clot Lysis ◽  
Platelet Factor ◽  
Treatment Groups ◽  
Vein Thrombosis ◽  
Significant Difference ◽  
At Iii ◽  
Euglobulin Clot Lysis Time ◽  
Group A ◽  
Uptake Test ◽  
Group B

We have evaluated the response of certain haema to logical parameters in patients undergoing elective thoracotomy for pulmonary and oesophageal disease. These patients are part of a continuing DVT study in which prophylaxis is randomised to either i) Heparin 5000 iu 8 hourly s.c. (Group A) or ii) Heparin 2500 iu + Dihydroergotamine (DHE) 0.5 mg 8 hourly s.c. (Group B). Prophylaxis was commenced at the time of premedication and continued for 10 days. DVT was diagnosed by I125 Fibrinogen uptake test, with confirmatory venography if positive. The haema to logical parameters investigated were Antithrombin III (AT III), Euglobulin Clot Lysis Time (ECLT), plasma $-Thromboglobulin (β-Tg), platelet Factor 4 (PF4) and Heparin level. All investigations were performed pre-op. and day 6 post-op. (AT III and ECLT were also measured on day 3).A total of 50 patients have completed the study to date with 25 patients in each treatment group. Eleven patients have developed DVT - 5 in Group A and 6 in Group B. There was no significant difference between the pre-op. values in either group. The post-op. AT III values of patients developing DVT were lower than the two treatment groups. ECLT was significantly prolonged in the two treatment groups post-op. compared to the pre-op. values (p < 0.05 (A) and p < 0.01 (B)), but was not prolonged post-op. in the patients who developed DVT. Plasma β-Tg and PF4 were raised pre-op. with further increases post-op. in all groups. Plasma Heparin concentrations in Group B (0.122 iu/ml) were lower than for Group A (0.182 iu/ml) and the DVT group (0.176 iu/ml).These findings indicate that none of the haematological investigations were able to delineate ‘at risk’ patients. However the development of DVT was significantly correlated (p < 0.05) with the post-op. AT III and ECLT. The use of DHE in the dose cited above did not apparently inhibit the platelet release reaction

DeVega versus ring annuloplasty in severe functional tricuspid insufficiency and their impact on the right ventricle

2019 ◽  
Vol 1 (4) ◽  
pp. 133-139
Author(s):  
Yasser Hamdy ◽  
Mohammed Mahmoud Mostafa ◽  
Ahmed Elminshawy
Keyword(s):  
Right Ventricular ◽  
Tricuspid Valve ◽  
Tricuspid Regurgitation ◽  
Early Postoperative Period ◽  
Tricuspid Valve Repair ◽  
Ring Annuloplasty ◽  
Significant Difference ◽  
Group A ◽  
Group B ◽  
The Right

Background: Functional tricuspid valve regurgitation secondary to left-sided valve disease is common. DeVega repair is simple, but residual regurgitation with subsequent impairment of the right ventricular function is a concern. This study aims to compare tricuspid valve repair using DeVega vs. ring annuloplasty and their impact on the right ventricle in the early postoperative period and after six months. Methods: This is a prospective cohort study of 51 patients with rheumatic heart disease who underwent tricuspid valve repair for secondary severe tricuspid regurgitation. Patients were divided into two groups: group A; DeVega repair (n=34) and group B; ring annuloplasty repair (n=17). Patients were assessed clinically and by echocardiography before discharge and after six months for the degree of tricuspid regurgitation, right ventricular diameter and tricuspid annular plane systolic excursion (TAPSE). Results: Preoperative echocardiographic assessment showed no difference in left ventricular end-systolic diameter, end-diastolic diameter, ejection fraction and right ventricular diameter, however; group A had significantly better preoperative right ventricular function measured by TAPSE (1.96 ± 0.27 vs1.75 ± 0.31 cm; p=0.02). Group B had significantly longer cardiopulmonary bypass time (127.65 ± 13.56 vs. 111.74 ± 18.74 minutes; p= 0.003) and ischemic time (99.06 ± 11.80 vs. 87.15 ± 16.01 minutes; p= 0.009). Pre-discharge, there was no statistically significant difference in the degree of tricuspid regurgitation, but the right ventricular diameter was significantly lower in group B (2.66 ± 0.41 and 2.40 ± 0.48 cm; p=0.049). After six months of follow up, the degree of tricuspid regurgitation (p= 0.029) and the right ventricular diameter were significantly lower in the ring annuloplasty group (2.56 ± 0.39 and 2.29 ± 0.44 cm; p=0.029). Although there was a statistically significant difference in preoperative TAPSE, this difference disappeared after six months. Conclusion: Both DeVega and ring annuloplasty techniques were effective in the early postoperative period, ring annuloplasty was associated with lesser residual regurgitation and better right ventricular remodeling in severe functional tricuspid regurgitation than DeVega procedure after 6-months of follow up.

Effect of short-term use of oral contraceptive pills on intraocular pressure

2020 ◽  
Vol 35 (3) ◽  
Author(s):  
Tayyaba Gul Malik ◽  
Hina Nadeem ◽  
Eiman Ayesha ◽  
Rabail Alam
Keyword(s):  
Oral Contraceptive ◽  
Childbearing Age ◽  
Short Term ◽  
Oral Contraceptive Pills ◽  
Contraceptive Pills ◽  
Time Period ◽  
Significant Difference ◽  
Group A ◽  
Group B ◽  
Short Time

Objective: To study the effect of short-term use of oral contraceptive pills on intra-ocular pressures of women of childbearing age.   Methods: It was a comparative observational study, conducted at Arif memorial teaching hospital and Allied hospital Faisalabad for a period of six months. Hundred female subjects were divided into two groups of 50 each. Group A, included females, who had been taking oral contraceptive pills (OCP) for more than 6 months and less than 36 months. Group B, included 50 age-matched controls, who had never used OCP. Ophthalmic and systemic history was taken. Careful Slit lamp examination was performed and intraocular pressures (IOP) were measured using Goldman Applanation tonometer. Fundus examination was done to rule out any posterior segment disease. After collection of data, we analyzed and compared the intra ocular pressures between the two groups by using ANOVA in SPSS version 21.   Results: Average duration of using OCP was 14.9 months. There was no significant difference of Cup to Disc ratios between the two groups (p= 0.109). However, significant difference was noted between the IOP of OCP group and controls. (p=0.000). Conclusion: OCP significantly increase IOP even when used for short time period.

Maximum Temperature Through a Yttrium Stabilized Zirconia Ceramic After Er,Cr:YSGG Laser Irradiation

2020 ◽  
Vol 02 ◽  
Author(s):  
Laurel Stringer ◽  
Sarah Malley ◽  
Darrell M. Hutto ◽  
Jason A. Griggs ◽  
Susana M. Salazar Marocho
Keyword(s):  
High Speed ◽  
Potential Method ◽  
Maximum Temperature ◽  
Zirconia Ceramic ◽  
Tooth Pulp ◽  
Control Group ◽  
Stabilized Zirconia ◽  
Significant Difference ◽  
Power Setting ◽  
Group A

Background: The most common approach to remove yttria stabilized zirconia (YSZ) fixed-dental prostheses (FDPs) is by means of diamond burs attached to a high-speed handpiece. This process is time-consuming and destructive. The use of lasers over mechanical instrumentation for removal of FDPs can lead to efficient and predictable restoration retrievability. However, the heat produced might damage the tooth pulp (>42˚C). Objective: The purpose of this study was to determine the maximum temperature (T) reached during the use of different settings of the erbium, chromium:yttrium-scandium-gallium-garnet Er,Cr:YSGG laser through a YSZ ceramic. Methods: YSZ slices (1 mm thick) were assigned into 7 groups. For the control group, a diamond bur was used to cut a 1 mm groove into the YSZ slices. For the 6 experimental groups, the laser was operated at a constant combination of 33% water and 66% air during 30 s with two different power settings (W) at three frequencies (PPS), as follows (W/PPS): 2.5/20, 2.5/30, 2.5/45, 4.5/20, 4.5/30, 4.5/45. The T through the YSZ slice was recorded in degrees Celsius by using a digital thermometer with a K thermocouple. Results: The median T of the control group was 26.5˚C. The use of 4.5 W resulted in the median T (˚C) of 44.2 at 20 PPS, 53.3 at 30 PPS, and 58.9 at 45 PPS, while 2.5 W showed 34.6, 31.6, and 25.0 at 20, 30, and 45 PPS, respectively. KruskalWallis one-way ANOVA showed that within each power setting, the T was similar. The high power and lowest frequency (4.5/20) showed no significant difference from the 2.5 W settings and the control group. Conclusion: The lower power setting (2.5 W) is a potential method for the use of the Er,Cr:YSGG laser to debond YSZ structures. The higher power (4.5 W) with high frequencies (30 and 45 PPS) is unsuitable.

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