Antimicrobial effect of essential oil isolated from Eucalyptus globulus Labill. from Montenegro

Chemical composition of the essential oil of Eucalyptus globulus Labill., grown in Montenegro, was analysed by gas chromatography-mass spectrometry and its antimicrobial activity was evaluated against 17 microorganisms, including food poisoning and spoilage bacteria and human pathogens.The Eucalyptus essential oil yield was 1.8% (w/w) on the fresh weight basis, whereas the analysis resulted in the identification of a total of 11 constituents, 1.8 cineole (85.8%), α-pinene (7.2%), and β-myrcene (1.5%) being the main components. Other compounds identified in the oil were β-pinene, limonene, α-phellandrene, γ-terpinene, linalool, pinocarveol, terpinen-4-ol, and α-terpineol. The results of the antimicrobial activity tests revealed that the essential oil of E. globulus has rather a strong antimicrobial activity, especially against Streptococcus pyogenes, Escherichia coli, Candida albicans, Staphylococcus aureus, Acinetobacter baumannii, and Klebsiella pneumoniae. Minimum inhibitory concentration revealed the lowest activity against Pseudomonas aeruginosa and Salmonela infantis (3.13 mg/ml) while the highest activity was against S. aureus, E. coli, and S. pyogenes (0.09 mg/ml).

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Comparison of the Chemical Composition and Antimicrobial Activity of Thymus serpyllum Essential Oils

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha4329899 ◽

2015 ◽

Vol 43 (2) ◽

pp. 432-438 ◽

Cited By ~ 5

Author(s):

Aneta WESOŁOWSKA ◽

Monika GRZESZCZUK ◽

Dorota JADCZAK ◽

Paweł NAWROTEK ◽

Magdalena STRUK

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Micrococcus Luteus ◽

Gas Chromatography Mass Spectrometry ◽

E Coli ◽

Thymus Serpyllum ◽

Main Components ◽

Total Oil

The chemical composition of the essential oils obtained by hydrodistillation from the aerial parts of Thymus serpyllum and Thymus serpyllum‘Aureus’ has been investigated by gas chromatography-mass spectrometry (GC-MS). Forty-seven compounds (99.67% of the total oil) wereidentified in the essential oil of T. serpyllum. The main components found in the oil were carvacrol (37.49%), -terpinene (10.79%), -caryophyllene (6.51%), p-cymene (6.06%), (E)--ocimene (4.63%) and -bisabolene (4.51%). Similarly, carvacrol (44.93%), -terpinene(10.08%), p-cymene (7.39%) and -caryophyllene (6.77%) dominated in the oil of T. serpyllum ‘Aureus’. A total of forty three compounds wereidentified in this oil, representing 99.49% of the total oil content. On the basis of the obtained data it was proved that the content of 1-octen-3-ol,eucalyptol, (Z)--ocimene, (E)--ocimene, -terpinene, carvacrol methyl ether, germacrene D and -bisabolene was significantly higher for T.serpyllum while T. serpyllum ‘Aureus’ was characterized by a significantly higher content of 3-octanone, 3-octanol, p-cymene, borneol andcarvacrol. The isolated essential oils were evaluated for their antimicrobial activity against nine reference strains (Escherichia coli, Staphylococcusaureus, Staphylococcus epidermidis, Streptococcus agalactiae, Enterococcus faecalis, Bacillus cereus, Micrococcus luteus, Proteus vulgaris and Candidaalbicans) by the microdilution technique. Based on this test, the minimum inhibitory concentrations (MIC) of essential oil were calculated. Thevolatile oil obtained from T. serpyllum showed the highest antimicrobial activity relative to the strain of E. coli (MIC=0.025 μL/mL) and to theyeast C. albicans (MIC=0.05 μL/mL). Similarly, a significant antimicrobial activity exhibited T. serpyllum ‘Aureus’ essential oil, although the MICvalues obtained in that case for E. coli and C. albicans strains were twice as high and were respectively 0.05 μL/mL and 0.1 μL/mL.

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GC/MS Analysis and Antimicrobial Activity of the Essential Oil of Fresh Leaves of Eucalytus Globulus, and Leaves and Stems of Smyrnium Olusatrum from Constantine (Algeria)

Natural Product Communications ◽

10.1177/1934578x1000501031 ◽

2010 ◽

Vol 5 (10) ◽

pp. 1934578X1000501 ◽

Cited By ~ 5

Author(s):

Habiba Daroui-Mokaddem ◽

Ahmed Kabouche ◽

Mabrouka Bouacha ◽

Boudjemaa Soumati ◽

Aida El-Azzouny ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Essential Oils ◽

Eucalyptus Globulus ◽

Bacterial Strains ◽

Ms Analysis ◽

Wide Range ◽

Main Components ◽

Type Apparatus

The essential oils of fresh leaves of Eucalyptus globulus L. (Myrtaceae), and leaves and stems of Smyrnium olusatrum L. (Apiaceae), obtained by hydrodistillation in a Clevenger-type apparatus, were analyzed by GC/MS. Twenty compounds were characterized, representing 98.3% of the essential oil of E. globulus, with 1,8-cineole (48.6%), globulol (10.9%), trans-pinocarveol (10.7%), and α-terpineol (6.6%) as main components, while thirty-three compounds were characterized, representing 94.3% of the essential oil of S. olusatrum, with sabinene (27.1%), curzerene (13.7%), methyl-1-benzyl-2-oxocyclooctane carboxylate (12.3%), α-pinene (7.2%), cryptone (7.1%) and β-pinene (5.7%) as the major components. The essential oils of E. globulus and S. olusatrum were tested against a wide range of fungal and bacterial strains. Both oils showed significant antimicrobial activity.

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Monitoring of Antimicrobial Activity of Essential Oils Using Molecular Markers

The Open Biotechnology Journal ◽

10.2174/1874070700903010103 ◽

2009 ◽

Vol 3 (1) ◽

pp. 103-107 ◽

Cited By ~ 3

Author(s):

Hend A. Hamedo

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Essential Oils ◽

Antimicrobial Agents ◽

Random Amplified Polymorphic Dna ◽

Antimicrobial Effect ◽

E Coli ◽

Specific Effects ◽

And Staphylococcus Aureus ◽

Rosemary Essential Oil

Technological application of essential oils, as natural antimicrobial agents, to reduce the effect of pathogenic microorganisms, requires new methods of detection. The present work evaluated the parameters of antimicrobial activity of the essential oils of rosemary (Rosmarinus officinalis) on two pathogenic strains Escherichia coli and Staphylococcus aureus. The MBC and MIC values were of 2.5, 25 μl ml-1, and values of 1.25 and 5 μl ml-1 for the two strains respectively. In this study, an attempt has been made to evaluate randomly amplified polymorphic DNA (RAPD) analysis for its potential to establish antimicrobial effect of rosemary essential oil. For the preliminary assessment, this study compared the effects occurring at molecular levels in E. coli and Staph. aureus exposed to rosemary essential oil at the MIC concentrations for the two organisms. The qualitative modifications arising in random amplified polymorphic DNA (RAPD) profiles as a measure of DNA effects were compared with control which showed many differences. In conclusion, the measurement of parameters at molecular levels is valuable for investigating the specific effects of agents interacting with DNA.

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Chemical Composition and Antimicrobial Activity of the Essential Oil From Euphorbia helioscopia L.

Natural Product Communications ◽

10.1177/1934578x20953249 ◽

2020 ◽

Vol 15 (9) ◽

pp. 1934578X2095324

Author(s):

Qing Zhu ◽

Mei-Lin Jiang ◽

Feng Shao ◽

Guang-Qiang Ma ◽

Qiang Shi ◽

...

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Shigella Dysenteriae ◽

Gas Chromatography Mass Spectrometry ◽

E Coli ◽

Aerial Parts ◽

Euphorbia Helioscopia ◽

Total Oil ◽

Strong Antimicrobial Activity

The chemical composition and antimicrobial activity of the essential oil from the aerial parts of Euphorbia helioscopia L. were investigated by gas chromatography-mass spectrometry (GC-MS), GC, and microdilution methods. Thirty-five compounds, representing 83.51% of the total oil, were identified. 1,6-Dihydrocarveol (31.39%), carvone (16.79%), menthol (8.23%), and trans-dihydrocarvone (5.53%) were the principal constituents of the oil. The essential oil exhibited strong antimicrobial activity against strains of the bacteria, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Shigella dysenteriae, and a strain of the fungus Candida albicans with a minimal inhibitory concentration value of 31.25 μg/mL and minimum bactericidal concentration values of 31.25 μg/mL ( S. aureus), 62.50 μg/mL ( E. faecalis), >62.50 μg/mL ( E. coli), >62.50 μg/mL ( S. dysenteriae), and >62.50 μg/mL ( C. albicans). These results indicated that the essential oil from the aerial parts of E. helioscopia could be used to control diseases caused by these microbes.

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Antimicrobial Activity of Essential Oil from Seeds of Carum carvi and Its Composition

Bangladesh Journal of Microbiology ◽

10.3329/bjm.v25i2.4867 ◽

1970 ◽

Vol 25 (2) ◽

pp. 85-89 ◽

Cited By ~ 7

Author(s):

Jaripa Begum ◽

M Nazrul Islam Bhuiyan ◽

Jasmin Uddin Chowdhury ◽

M Nuzmul Hoque ◽

M Nural Anwar

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Pathogenic Bacteria ◽

Inhibitory Concentration ◽

Antimicrobial Activities ◽

Phytopathogenic Fungi ◽

Gas Chromatography Mass Spectrometry ◽

Minimum Fungicidal Concentration ◽

Carum Carvi ◽

Main Components

The essential oil of Carum carvi L. seeds was screened for its antimicrobial activity against ten pathogenic bacteria and six phytopathogenic fungi. The essential oil showed promising inhibitory activity against all the test bacteria, even at 2 ìl/disc. The minimum inhibitory concentration (MIC, 100-300 ppm) and minimum bactericidal concentration (MBC, 200-400 ppm) values of essential oil were determined. The antifungal screening of the essential oil showed 100% inhibition of radial mycelial growth of all the test fungi at 100 ppm. The MIC and minimum fungicidal concentration (MFC) values were found to vary from 50-300 ppm and 200-400 ppm respectively. The essential oil extracted by hydrodistillation from seeds of C. carvi was analyzed by gas chromatography-mass spectrometry (GC-MS). About 10 compounds had been identified in the seeds oils, accounting for more than 99.7% of the oils. The main components of the seeds oil were thymol (48.20%), o-cymene (19.29%), ϒ-terpinen (17.61%) and trimethylene dichloride (8.81%). Keywords: Antimicrobial activities; Carum carvi; Essential oil; GC-MS; ThymolDOI: http://dx.doi.org/10.3329/bjm.v25i2.4867 Bangladesh J Microbiol, Volume 25, Number 2, December 2008, pp 85-89

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Chemical composition and biological activity of Peucedanum dhana A. Ham essential oil

Scientific Reports ◽

10.1038/s41598-021-98717-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Sarunpron Khruengsai ◽

Teerapong Sripahco ◽

Narawadee Rujanapun ◽

Rawiwan Charoensup ◽

Patcharee Pripdeevech

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Membrane Integrity ◽

Enterobacter Aerogenes ◽

Inhibition Zone ◽

Gas Chromatography Mass Spectrometry ◽

E Coli ◽

Murine Fibroblast ◽

Pure Compounds ◽

Lung Adenocarcinoma A549

AbstractThe essential oil was extracted from Peucedanum dhana A. Ham, which grows in Thailand, using a Clevenger apparatus, resulting in an oil yield of 0.76% w/w. Forty-two compounds were identified using gas chromatography-mass spectrometry. The major compounds were trans-piperitol (51.23%), β-pinene (11.72%), o-cymene (11.12%), γ-terpinene (9.21%), and limonene (4.91%). The antimicrobial activity of the P. dhana essential oil was investigated by measuring the inhibition zone diameter, minimum inhibitory concentration (MIC), and minimum microbicidal concentration (MMC). The inhibition zone diameters of P. dhana essential oil (1000 µg/mL) against tested pathogens ranged from 10.70 to 40.80 mm. Significant antimicrobial activity against tested pathogens was obtained, with MIC and MMC values of 62.50–250 µg/mL and 250–1000 µg/mL, respectively. Escherichia coli, Pseudomonas aeruginosa, and Enterobacter aerogenes exposed to P. dhana essential oil at the MIC were analysed by flow cytometry using propidium iodide (PI) and SYTO9 to assess membrane integrity compared to trans-piperitol and β-pinene. After 24 h, treatments with trans-piperitol resulted in the most significant cell membrane alteration and depolarization followed by P. dhana essential oil and β-pinene, respectively. It was demonstrated that the P. dhana essential oil presented antibacterial action against E. coli, P. aeruginosa, and E. aerogenes. The antioxidant activity of P. dhana essential oil was measured using 2,2-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium (ABTS) scavenging activity assays. The IC50 values obtained from the DPPH and ABTS methods were 9.13 and 9.36 mg/mL, respectively. The cytotoxic effect of P. dhana oil was tested against human colonic adenocarcinoma (SW480), human lung adenocarcinoma (A549), cervical cancer (Hela), and murine fibroblast (3T3L1) cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The essential oil had cytotoxicity against all cancer cells, with significant cytotoxicity towards SW480 cells. As a control experiment, two pure compounds—trans-piperitol and β-pinene, were also tested for their antimicrobial, antioxidant, and cytotoxic activity. Both compounds showed varied activity in all assays. The results indicate that P. dhana essential oil could be used as a source of functional ingredients in food and pharmaceutical applications.

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Extracts and essential oil of two medicinal plants as a candidate against urinary tract infection caused by multi-drug resistance E. coli

10.21203/rs.3.rs-81445/v1 ◽

2020 ◽

Author(s):

Mohammad Ahmadian ◽

Mohammad Javad Mehdipour Moghaddam

Keyword(s):

Antimicrobial Activity ◽

Urinary Tract ◽

Essential Oil ◽

High Performance ◽

Gas Chromatography Mass Spectrometry ◽

Multi Drug Resistance ◽

Food Preservatives ◽

E Coli ◽

Tract Infections ◽

Eryngium Caucasicum

Abstract Background: The antimicrobial activity of ethanolic and aqueous extracts of the two plants, including Eryngium caucasicum Trautv (EtEc, AqEc) and Froriepia subpinnata (EtFs, AqFs), as well as the essential oil of Eryngium caucasicum (EoFs) on MDR E. coli spp. isolated from the urine samples were examined. Methods: Microdilution and agar well diffusion methods were used to determine the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and inhibition zone diameter of the extracts and essential oil. Reverse-phase High- performance liquid chromatography (RP-HPLC) and gas chromatography-mass spectrometry (GC-mass) were applied for phytochemical analyses of extracts and essential oil ingredients. Results: The inhibitory rate based on MIC of AqEc, EtEc, AqEc, EtEc and EoEs on total isolates were 37.6%, 43%, 31.2%, 40.9% and 53.8%, respectively. The killing rate based on MBC of AqEc, EtEc, AqEc, EtEc and EoFs on total isolates were 15.1%, 20.4%, 12.9%, 24.7% and 34.4%, respectively. EoFs, with the exception of cephalothin, chloramphenicol, pipiracillin and carbapenems, can be used as an alternative to 12 of the 18 antibiotics used (P<0.01). Compounds with antimicrobial activity including quercetin, catechin, terpenes, alcohols and aromatic molecules in extracts and essential oils have been identified and measured, but chlorogenic acid polyphenol could not be identified in any of the plant products. Conclusion: It seems that the extracts and essential oil studied have the potential to be used as native materials to treat urinary tract infections as well as food preservatives or other products.

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AKTIVITAS ANTIMIKROBA MINYAK ESENSIAL JAHE MERAH (Zingiber ofﬁcinale var. Rubrum) DAN LENGKUAS MERAH (Alpinia purpurata K. Schum) TERHADAP BAKTERI PATOGEN DAN PERUSAK PANGAN

Jurnal Agritech ◽

10.22146/agritech.9418 ◽

2015 ◽

Vol 35 (01) ◽

pp. 43 ◽

Cited By ~ 5

Author(s):

Tita Rialita ◽

Winiati Pudji Rahayu ◽

Lilis Nuraida ◽

Budi Nurtama

Keyword(s):

Antimicrobial Activity ◽

Refractive Index ◽

Essential Oil ◽

Essential Oils ◽

Optical Rotation ◽

Acid Number ◽

Food Spoilage ◽

E Coli ◽

Spoilage Bacteria ◽

Red Ginger

The aims of this study was to determine the characteristics, composition and antimicrobial activity of essential oils of local Indonesian red ginger and red galangal against four pathogenic and food spoilage bacteria, which were B.cereus ATCC 10876, E. coli ATCC 25922, S. typhimuriumATCC 14028, and P. aeruginosa ATCC 27853. Analysis of physicochemical characteristics was carried outin accordance with ISO7355:1985. The chemical compositionwas analyzed using aGC-MS. The antimicrobial activity was determined by disc diffusion method and broth microdillution method was used for determine MIC and MBC values. Red ginger essential oil characteristic was brownish yellow, speciﬁc gravity 0.883, refractive index 1.480, optical rotation -8.45o, clear soluble (1:1) in 90 % alcohol, 2.06 acid number and 42.45 ester number. Redgalangal essential oil had a characteristic bright yellow color, speciﬁc gravity 0.895, refractive index 1.496, optical rotation -9.15o, clear soluble (1:1) in 90 % alcohol, 1.95 acid number and 140.15 ester number. The major component of red ginger essential oils were trimethyl-heptadien-ol, ar-curcumene, camphene, carbaldehyde, -sesquiphellandrene, and nerol; while the major component of red galangal essential oil were 1.8-cineole, chavicol, 9-desoxo-9-xi-hydroxy-3-pentaacetate-3,5,7,8,9,12-Ingol,- caryophyllene and -selinene. The essential oil of red ginger and red galangal hadmoderate antibacterial activity against pathogenic and food spoilage bacteria with the average inhibition zone 7.17-10.33 and 7.25-11.17mm.Red ginger essential oils could inhibit the growth of tested bacteria with MIC values of 2.65-3.97 mg/mL and MBC value of 3.10-5.29 mg/mL, while the red galangal essential oil could inhibit the growth of tested bacteria with MIC values of 1.79-4.03 mg mL and MBC values of 1.79-4.92 mg/mL. Based on the MIC and MBC values,all tested bacteriasensitivity to essential oils of red ginger and galangal red decline in a row B.cereus > E. coli > S. typhimurium> P. aeruginosa. Sensitivity of Gram positive and Gram negative bacteria to both essential oils demonstrate the potential of the oils to be used as a natural preservative in the food industry.Keywords: Antimicrobial, essential oil, red ginger, red galangal ABSTRAKPenelitian ini bertujuan untuk mengetahui karakteristik, komposisi dan aktivitas antimikroba minyak esensial jahe merah dan lengkuas merah lokal Indonesia terhadap empat spesies bakteri patogen dan perusak pangan, yaitu B.cereus ATCC 10876, E.coli ATCC 25922, S. typhimurium ATCC 14028, dan P. aeruginosa ATCC 27853. Analisis karakteristikﬁsika-kimia dilakukan sesuai standar ISO 7355:1985. Komposisi kimia dianalisis menggunakan alat GC-MS. Pengujian aktivitas antimikroba dilakukan dengan metode difusi cakram untuk menentukan zona hambat, sertabroth microdillution untuk menentukan nilai Minimum Inhibitory Concentration (MIC) dan Minimum Bactericidal Concentration (MBC).Karakteristik minyak esensial jahe merah yang dihasilkan yaitu kuning kecoklatan, berat jenis 0,883, indeks bias 1,480, putaran optik -8.45, larut jernih (1:1) dalam alkohol 90%, bilangan asam 2,06, dan bilangan ester 42,45. Minyak esensial lengkuas merah memiliki karakteristik warna kuning terang, berat jenis 0,895, indeks bias 1,496, putaran optik-9.15, larut jernih (1:1) dalam alkohol 90%, bilangan asam 1,95 dan bilangan ester 140,15. Komponen mayor minyak esensial jahe merah terdiri dari trimethyl-heptadien-ol, ar-curcumene, camphene, carbaldehyde, -sesquiphellandrene, dan nerol; sedangkan komponen mayor minyak esensial lengkuas merah terdiri dari 1.8-cineole, chavicol,9-desoxo9-xi-hydroxy-3,5,7,8,9,12-pentaacetat-ingol, -caryophyllenedan -selinene. Minyak esensial jahe merah dan lengkuas merah memiliki aktivitas antibakteri yang bersifat moderat terhadap bakteri patogen dan perusak pangan, dengan kisaran zona hambat rata-rata 7,17-10,33 mm dan 7,25-11,17 mm. Minyak esensial jahe merah dapat menghambatpertumbuhan bakteri uji pada nilai MIC 2,65-3,97 mg/mL dan nilai MBC 3,10-5,29 mg/mL, sedangkan minyak esensial lengkuas merah dapat menghambat bakteri uji dengan nilai MIC 1,79-4,03 mg/mL dan nilai MBC 1,79-4,92 mg/mL. Berdasarkan nilai MIC dan MBC, sensitivitas bakteri uji terhadap minyak esensial jahe merah dan lengkuas merah menurun berturut-turut dari B. cereus > E. coli > S. typhimurium > P. aeruginosa. Sensitivitas bakteri Gram positif dan Gram negatif terhadap kedua minyak esensial ini menunjukkan potensi minyak esensial jahe merah dan lengkuas merah untuk digunakan sebagai pengawet alami di industri pangan.Kata kunci: Antimikroba, jahe merah, lengkuas merah, minyak esensial

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Amtimicrobial activity of essential oil of Melissa officinalis L, Lamiaceae

Hemijska industrija ◽

10.2298/hemind0510243a ◽

2005 ◽

Vol 59 (9-10) ◽

pp. 243-247 ◽

Cited By ~ 2

Author(s):

Nada Anicic ◽

Suzana Dimitrijevic ◽

Mihailo Ristic ◽

Slobodan Petrovic ◽

Slobodan Petrovic

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Bacterial Species ◽

Antimicrobial Properties ◽

Dilution Method ◽

Melissa Officinalis ◽

E Coli ◽

Diffusion Technique ◽

Main Components ◽

Broth Dilution

The antimicrobial activity of the essential oil of Melissa officinalis was investigated in this paper. The essential oil was obtained by the principle of water and steam and analyzed by GC and GC-MS using FID and MSD. The main components of the oil of Melissa officinalis were geranial (17.30%), neral (14.70%) and citronellal (10.70%). The antimicrobial properties were tested against the following bacterial species: B subtilis, B.cereus Bifidobacterium sp., Corynobacterium sp., E. coli, Klebsiella sp., L. acidophilus, L. fermentum, L. plantarum, L. rhamnosus, Listeria monocytogenes, P. vulgaris, P. aerugenosa, S. enteritidis, Shigella sp., S. aureus, and fungi Candida albicans, Alternarija sp. and Aspergillus niger. The diffusion technique was used for testing: the antimicrobial activity, and the MIC was determined by the broth dilution method. The essential oil of M. officinalis showed high antimicrobial activity.

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ANTIMICROBIAL ACTIVITY OF OIL-BEARING PLANTS LAMIACEAE LINDL. TOWARDS ESCHERICHIA COLI

Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University ◽

10.15421/201612 ◽

2016 ◽

Vol 6 (01) ◽

pp. 216-236

Author(s):

L.A. Kotyuk

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Essential Oil ◽

Antimicrobial Properties ◽

Biologically Active ◽

E Coli ◽

Twofold Increase ◽

Ethanol Extracts ◽

Main Components ◽

Active Substances

The paper relates to study of biological activity of 40% ethanol extracts of Dracocephalum moldavica, Hyssopus officinalis, Satureja hortensis, Lophanthus anisatus and Monarda diduma, grown in Ukrainian Polissya, against a pathogenic agent Escherichia coli UCM – B (ATCC 25922).The research proves that ethanol extracts of H. officinalis, D. moldavica, S. hortensis, L. anisatus exert antimicrobial activity as the extracted substances provided a twofold increase in minimum bactericidal concentration (MBC) values against E. coli. Likewise, a twofold increase was observed in minimum inhibitory concentration (MIC) of L. anisatus ethanol extracts. As to M. diduma ethanol extracts, their inhibitory and bactericidal influence on E. coli was not registered.Oil-bearing plants (family Lamiaceae), grown in Zhytomyr Polissya, are characterized by antimicrobial properties, attributed to biologically active substances that are formed and accumulated in the plant material. The main components of hyssop essential oil are isopinocamphone (44.43%), pinocamphone (35.49%), myrtenol (5.26 %), germacrene D (3.15 %), pulegone (2.93 %), bicyclogermacrene (1.35 %). In mint anise essential oil prevailed pulegone (59.19%), izomenton (14.34%), bicyclogermacrene (3,21 %), β-kariofilen (2,99 %), menton (2.21 %), 1,6-germacradien-5-ol (1.5 %), isopulegone (1.4 %), in summer savory – carvacrol (89.07%), g-terpinene (3.53%), α-thujone (1.7 %), camphor (1.48 %). The dominant components of moldavian dragonhead essential oil were geranial (26.19%) and neral (22.36%), 2-(1-hydroxy-1-isopropyl)-cyklopentanon (8.29 % ), 2,3-dehydro-1,8-cineole (6.87 %), 3-(1-hydroxy-1-isopropyl) cyklopentanon (6,51 %), nerol (4.74 %), 3-methyl-2-cyclohexane 1-on (2.13 %).The paper draws attention to further more detailed study of ethanol extracts of hyssop, moldavian dragonhead, summer savory, mint anise with the aim of producing antibacterial herbal preparations.

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