scholarly journals Morphological and morphometric adaptations of testes in broilers induced by glucocorticoid

2021 ◽  
Author(s):  
R Islam ◽  
N Sultana ◽  
U Ayman ◽  
A Akter ◽  
M Afrose ◽  
...  
Keyword(s):  
Seminiferous Tubule ◽  
Seminiferous Epithelium ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Treatment Groups ◽  
Commercial Broiler ◽  
Capsule Thickness ◽  
Morphology And Morphometry ◽  
Testicular Capsule ◽  
Tubule Diameter

Glucocorticoids (GCs) cause excess fat accumulation, which leads to fertility dysfunction in broilers. The study investigated alterations in the morphology and morphometry of the testes of broilers in response to GC and dexamethasone (DEX). Male day-old chicks were randomly divided into a control group and three experimental groups (E1, E2, and E3). The control group was fed a commercial broiler ration. The experimental groups were fed a commercial broiler ration containing GC (i.e. DEX 3, 5, and 7 mg/kg, respectively). The testes were collected and stained with haematoxylin and eosin to count the number of testicular seminiferous tubules. An increase in the seminiferous tubule count was initially seen, which declined as both the age of the broilers and the dose of DEX increased. Morphometric measurements, i.e., the testicular capsule thickness, seminiferous tubule diameter, and seminiferous epithelium height, were performed. The initial thickening of the testicular capsule was evident. There was a depletion of the interstitial (Leydig) cell population in the experimental groups with the age and increased with the dose advancement. The diameter of the seminiferous tubules and testicular capsule thickness remained upregulated in the treatment groups with the increased dose of DEX. The initial height of the seminiferous epithelium increased in the experimental groups of broilers. The study suggests that DEX greatly alters the morphological architecture of broiler testes; as a result, it could be said that DEX has the effect on the infertility of the broiler by affecting the morphology as well as the functionality of the testes.

scholarly journals Protective effects of curcumin against methotrexate-induced testicular damage in rats by suppression of the p38-MAPK and nuclear factor-kappa B pathways

2021 ◽  
Author(s):  
Leyla Kilinc ◽  
Yesim Hulya Uz
Keyword(s):  
Body Weight ◽  
P38 Mapk ◽  
Seminiferous Tubule ◽  
Nuclear Factor Kappa B ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Nuclear Factor ◽  
Testicular Damage ◽  
Space Width ◽  
Tubule Diameter

Objective: Methotrexate (MTX), is a commonly used chemotherapeutic agent. This study aimed to investigate the possibility that curcumin (CMN) protects against MTX-induced testicular damage by affecting the phospho (p)-p38 mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-kB) signaling pathways. Methods: Male Wistar albino rats were subdivided into three groups. The control group was given an intragastric (ig) administration of dimethyl sulfoxide (DMSO) daily for 14 days, the MTX group was given a single intraperitoneal (ip) dose of MTX (20 mg/kg) on the 11th day, and the MTX+CMN group was given ig CMN (100 mg/kg/day, dissolved in DMSO) for 14 days and a single ip dose of MTX (20 mg/kg) on the 11th day. Results: The animal weights, the seminiferous tubule diameter, and germinal epithelium height significantly decreased in the MTX group compared to the control group. The testes weight and the ratio of the testes to body weight did not change, whereas the number of seminiferous tubules and the interstitial space width increased significantly in the MTX group. The number of phospho-p38 (p-p38) MAPK immunopositive cells and the immunoreactivity of NF-kB also increased in the MTX group compared to the control group. Conclusion: CMN prevented the MTX-induced decreases in the body weight, seminiferous tubule diameter, and the germinal epithelium height, while significantly reducing the number of histologically damaged seminiferous tubules and the interstitial space width changes due to MTX. CMN also reduced the number of p-p38 MAPK immunopositive cells and the NF-kB immunoreactivity.

scholarly journals Cyclic Expression of Endothelin-converting Enzyme-1 Mediates the Functional Regulation of Seminiferous Tubule Contraction

1999 ◽  
Vol 145 (5) ◽  
pp. 1027-1038 ◽  
Author(s):  
Antonella Tripiciano ◽  
Carmelina Peluso ◽  
Anna Rita Morena ◽  
Fioretta Palombi ◽  
Mario Stefanini ◽  
...  
Keyword(s):  
Sertoli Cell ◽  
Sertoli Cells ◽  
Seminiferous Tubule ◽  
Seminiferous Epithelium ◽  
Seminiferous Tubules ◽  
Converting Enzyme ◽  
Myoid Cells ◽  
Endothelin 1 ◽  
Endothelin Converting Enzyme ◽  
Cyclic Expression

The potent smooth muscle agonist endothelin-1 (ET-1) is involved in the local control of seminiferous tubule contractility, which results in the forward propulsion of tubular fluid and spermatozoa, through its action on peritubular myoid cells. ET-1, known to be produced in the seminiferous epithelium by Sertoli cells, is derived from the inactive intermediate big endothelin-1 (big ET-1) through a specific cleavage operated by the endothelin-converting enzyme (ECE), a membrane-bound metalloprotease with ectoenzymatic activity. The data presented suggest that the timing of seminiferous tubule contractility is controlled locally by the cyclic interplay between different cell types. We have studied the expression of ECE by Sertoli cells and used myoid cell cultures and seminiferous tubule explants to monitor the biological activity of the enzymatic reaction product. Northern blot analysis showed that ECE-1 (and not ECE-2) is specifically expressed in Sertoli cells; competitive enzyme immunoassay of ET production showed that Sertoli cell monolayers are capable of cleaving big ET-1, an activity inhibited by the ECE inhibitor phosphoramidon. Microfluorimetric analysis of intracellular calcium mobilization in single cells showed that myoid cells do not respond to big endothelin, nor to Sertoli cell plain medium, but to the medium conditioned by Sertoli cells in the presence of big ET-1, resulting in cell contraction and desensitization to further ET-1 stimulation; in situ hybridization analysis shows regional differences in ECE expression, suggesting that pulsatile production of endothelin by Sertoli cells (at specific “stages” of the seminiferous epithelium) may regulate the cyclicity of tubular contraction; when viewed in a scanning electron microscope, segments of seminiferous tubules containing the specific stages characterized by high expression of ECE were observed to contract in response to big ET-1, whereas stages with low ECE expression remained virtually unaffected. These data indicate that endothelin-mediated spatiotemporal control of rhythmic tubular contractility might be operated by Sertoli cells through the cyclic expression of ECE-1, which is, in turn, dependent upon the timing of spermatogenesis.

scholarly journals Effects of a standardized 40% ellagic acid pomegranate (Punica granatum L.) extract on seminiferous tubule histopathology, diameter, and epithelium thickness in albino Wistar rats after heat exposure

2019 ◽  
Vol 12 (8) ◽  
pp. 1261-1265
Author(s):  
Budi Utomo ◽  
Nurfitri Rahmah Daningtia ◽  
Gandul Atik Yuliani ◽  
Wiwik Misaco Yuniarti
Keyword(s):  
Ellagic Acid ◽  
Seminiferous Tubule ◽  
Wistar Rats ◽  
Punica Granatum ◽  
Histological Evaluation ◽  
Seminiferous Tubules ◽  
Acid Extract ◽  
Epithelium Thickness ◽  
Punica Granatum L ◽  
Tubule Diameter

Background and Aim: It has long been known that the spermatogenic tissue is very sensitive to temperatures higher than its physiologic temperature and causing cessation of activity and resulting in sterility. This study investigated the effect of a standardized 40% ellagic acid extract of pomegranate on the histopathology, diameter, and epithelial thickness of seminiferous tubules in albino rats exposed to heat. Materials and Methods: Twenty-five male albino Wistar rats were randomized at 7-8 months of age to five treatment groups. Group C was not treated; Group T0 was treated with 0.5% of Na carboxymethyl cellulose (CMC) 2 ml/day and exposed to heat. T1, T2, and T3 were treated with 75, 150, and 300 mg/kg/day of a standardized 40% ellagic acid extract of pomegranate (Punica granatum L.), respectively. The animals were orally administered Na CMC or pomegranate extract and were exposed to sunlight for 15 min at 40°C-42°C for 14 days. The animals were sacrificed on day 15 and the testes were removed for histological evaluation and measurement of seminiferous tubule diameter and epithelium thickness. Results: The diameter of seminiferous tubules from rats exposed to heat and treated with 300 mg/kg/day pomegranate extract was larger and the epithelia thicker than those in the other groups (p= <0.05). The protective effects of the standardized 40% ellagic acid extract may have been mediated by its antioxidant activity. Conclusion: Compared with controls, administration of 300 mg/kg/day of a standardized 40% ellagic acid extract of P. granatum L. for 14 days increased seminiferous tubule diameter and epithelium thickness in albino Wistar rats exposed to heat. Keywords: diameter, epithelium thickness, heat, pomegranate extract, seminiferous tubule.

scholarly journals Effect of Laserpuncture Shoot on Reproduction Point of Male Mojosari Duck (Anas Platyrhynchos) on The Numbers of Spermatogonium Cells and Seminiferous Tubules Diameter

2017 ◽  
Vol 3 (6) ◽  
pp. 718
Author(s):  
Melyandari Ayu Qomar ◽  
Rimayanti Rimayanti ◽  
Tri Nurhajati
Keyword(s):  
Seminiferous Tubule ◽  
Anas Platyrhynchos ◽  
Research Result ◽  
Seminiferous Tubules ◽  
Group Iii ◽  
Randomized Design ◽  
Group I ◽  
Histological Slide ◽  
Completely Randomized Design ◽  
Tubule Diameter

The Objective of this research was to know the effect of laserpuncture on reproduction point of male Mojosari duck on the number of spermatogonium cells and seminiferous tubule diameter of the testes. This research using completely randomized design with eighteen male Mojosari ducks which divided into three group randomly. Group I (P0) without any treatment of laserpuncture, Group II (P1) with 0.2 J dose of laserpuncture, and Group III (P2) with 0.4 J dose of laserpuncture. Laserpuncture shooting was done on reproduction point with three days interval in one month. On the thirty-first day, the duck was dissected so that the testes can be taken then made by the histological slide of testes. The obtained data processed by Analysis of Variant (ANOVA) followed with Duncan. Research result showed that laserpuncture shoot on the reproduction point of five-month-old male Mojosari duck decreases the number of spermatogonium cells and seminiferous tubule diameter of the testes. Keyword: laserpuncture, Mojosari duck, spermatogonium, seminiferous tubule

scholarly journals Effect of Nickel Administration in vivo on the Testicular Structure in Male Mice

2007 ◽  
Vol 76 (2) ◽  
pp. 223-229 ◽  
Author(s):  
P. Massányi ◽  
N. Lukáč ◽  
J. Zemanová ◽  
A. V. Makarevich ◽  
P. Chrenek ◽  
...  
Keyword(s):  
Body Mass ◽  
Seminiferous Tubule ◽  
Basal Membrane ◽  
Relative Volume ◽  
The Body ◽  
Germinal Epithelium ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Group B

The aim of this study was to describe the effects of nickel (NiCl2) on murine testicular structure. Experimental animals were injected intraperitoneally with a single dose of 20 mg NiCl2 per kg of body mass (group A, n = 5) and 40 mg NiCl2 per kg b. m. (group B, n = 5). The group without injection (n = 5) was the control (C). Animals were killed 48 hours after administration of nickel. The body mass of animals, the mass of testes and the testes : body mass ratio were not significantly affected. In both experimental groups a significant (p < 0.001) decrease of germinal epithelium in comparison with control group was observed. The relative volume of the interstitium was increased but not significantly in both experimental groups. An increase in the relative volume of the lumen was registered in both experimental groups in comparison with the control group. The qualitative analysis detected a dilatation of blood vessels in the interstitium, undulation of the basal membrane and several empty spaces in the germinal epithelium. The diameter (n = 150) of the seminiferous tubule was markedly (p < 0.05) decreased in both experimental groups (A, B) compared to control group. The height of the germinal epithelium showed a significant decrease (p < 0.05 - 0.001) after nickel administration. Evaluation of the lumen diameter in the seminiferous tubule showed a significant increase in both experimental groups. The data of the perimeter of seminiferous tubules corresponded with those of the seminiferous tubule diameter. TUNEL assay detected a higher frequency of localized apoptosis in the interstitium of nickel-administered animals compared to control group. Our findings clearly suggest a negative effect of nickel on the structure as well as on the function of the seminferous epithelium at the site of spermatozoa production.

scholarly journals The Effect of Inhibin Immunization in Seminiferous Epithelium of Yangzhou Goose Ganders: A Histological Study

Animals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2801
Author(s):  
Muhammad Faheem Akhtar ◽  
Ejaz Ahmad ◽  
Ilyas Ali ◽  
Muhammad Shafiq ◽  
Zhe Chen
Keyword(s):  
Germ Cells ◽  
Marked Reduction ◽  
Histological Study ◽  
Seminiferous Epithelium ◽  
Histological Evaluation ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Cell Numbers ◽  
Tubular Diameter ◽  
Reciprocal Expression

The current study investigated the effect of inhibin immunization on germ cell numbers (spermatogonia, spermatocytes, round, and elongated spermatids), seminiferous tubules (ST) diameter, Johnsen’s score, epithelial height (μm), luminal tubular diameter (μm), and number of ST per field (ST/field) of Yangzhou goose ganders. Histological evaluation showed apoptosis and regression of testes after inhibin (INH) immunization, with a concomitantly marked reduction in the round and elongated spermatids in the experiment (INH) group compared to the control group. The diameter of seminiferous tubules (ST) and epithelial height (EH) were positively correlated at 181, 200, and 227 days of age. In comparison, luminal tubular diameter (LD) was negatively correlated on day 227 to ST diameter and epithelial height. On day 227, many seminiferous tubules per field (ST/field) were negatively correlated to ST diameter, EH, and LD. INH immunization elevated ST diameter, EH, and LD, while Johnsen’s score and number of ST/field had reciprocal expression. In conclusion, the concomitant effect of INH immunization and seasonality in breeding regressed germ cells and damaged spermatogenesis in seminiferous epithelium Yangzhou ganders.

EFFECT OF SUPPLEMENTATION OF DIFFERENT LEVELS OF DRIED TAMARIND PULP ON MEAT CHOLESTEROL, CARCASS YIELD, DRESSING PERCENTAGE AND ABDOMINAL FAT OF BROILER CHICKEN

2016 ◽  
Vol 12 (1) ◽  
Author(s):  
P.V. Patil ◽  
G.B. Shinde ◽  
A.B. Kanduri ◽  
P.H. Pawar
Keyword(s):  
Broiler Chicken ◽  
Control Group ◽  
Broiler Chicks ◽  
Carcass Yield ◽  
Treatment Groups ◽  
Group I ◽  
Commercial Broiler ◽  
Dressing Percentage ◽  
Different Levels ◽  
Control Group Group

The present study was conducted to investigate the effect of supplementation of different levels of dried Tamarind pulp on meat cholesterol, carcass yield and dressing percentage of broiler chicken. 300 healthy day old commercial broiler chicks of Vencob’ strain were selected and divided into five groups of 60 chicks in each group, three replicates of 20 birds in each group. The chicks of control group (Group I) were fed with standard broiler diet without dried Tamarind pulp, whereas dried Tamarind pulp was supplemented @250 gm, 500 gm, 1000 gm and 1500 gm per 100 kg of broiler ration respectively to groups -II, III, IV and V.The meat cholesterol was found to be significantly lower in treatments groups than in control group. However, carcass yield and dressing percentage of treatment groups were significantly higher than control Group.The carcass yield and dressing percentage were increased and meat cholesterol level was decreased on supplementation of dried Tamarind pulp in broiler rations.

scholarly journals Visualization of Golgi complexes and spermatogonial cohorts of viable, intact seminiferous tubules.

1993 ◽  
Vol 41 (2) ◽  
pp. 299-306 ◽  
Author(s):  
K J Johnson ◽  
K Boekelheide
Keyword(s):  
Sertoli Cell ◽  
Golgi Complex ◽  
Seminiferous Tubule ◽  
Brefeldin A ◽  
Vital Staining ◽  
Staining Technique ◽  
Seminiferous Epithelium ◽  
Seminiferous Tubules ◽  
Simple Method ◽  
Peritubular Cells

Golgi complexes of cells within the intact, viable seminiferous tubule were examined by light microscopy using the vital Golgi stain C6-NBD-ceramide. This staining technique provided a quick and simple method to visualize all Golgi complexes of cells within the seminiferous tubule that are directly accessible to the basal compartment. Thus, peritubular, spermatogonial, and Sertoli cell Golgi complexes were visualized. Peritubular cells contained simple Golgi complexes which did not change with the stage of the seminiferous epithelium. Both solitary spermatogonial Golgi complexes which varied in size and number with the stage of the seminiferous epithelium and cohorts of spermatogonia connected by intercellular bridges were easily visualized. The Golgi complexes of Sertoli cells were located in the basal, perinuclear cytoplasm except in Stages VII-VIII, when they extended towards the lumen. Exposure of isolated seminiferous tubules to the fungal metabolite brefeldin A caused the Sertoli cell Golgi complex staining pattern to become diffuse or to co-localize with heads of elongate spermatids. The Golgi complexes of the peritubular cells and spermatogonia were resistant to brefeldin A. The C6-NBD-ceramide vital staining method should be useful for studying stage-dependent Sertoli cell Golgi complex movement and spermatogonial maturation.

scholarly journals Effects of Ribavirin on Thickness of Testicular Capsule in Albino Rats

BioMedica ◽  
2021 ◽  
Vol 37 (2) ◽  
pp. 123-129
Author(s):  
Alvia Batool ◽  
Maryam Fatima ◽  
Fozia Farzana
Keyword(s):  
Seminiferous Tubules ◽  
Control Group ◽  
Albino Rats ◽  
High Dose ◽  
Transient Effects ◽  
Mature Adult ◽  
Time Points ◽  
Mutagenic Property ◽  
Testicular Capsule ◽  
Different Doses

<p><strong>Background and Objective:</strong> Ribavirin (RBV), due to its mutagenic property, exerts cytotoxic effects on the testicular seminiferous tubules and its interstitium in various experimental animals. However, no study was done on the morphology of testicular capsule. Therefore, the present experimental study is designed to observe the transient effects of RBV in different doses on the testicular capsule thickness in albino rats.<br /><strong>Methods:</strong> A total of 72 sexually mature adult male albino rats were divided into four groups: A (control) and, B, C, D where RBV was administered intraperitoneally for 5 days in different doses (20, 100, and 200 mg/kg body weight, respectively). Group received distilled water intraperitoneally. Each group was further divided into three subgroups according to three sacrificial time points that were 20th, 40th, and 60th day from the last exposure to drug. Randomly selected rats from each group were sacrificed on every time point. Histological slides were prepared and changes were observed microscopically.<br /><strong>Results: </strong>On 20th sacrificial day, the increase in the thickness of capsules was seen in all groups with subcapsular edema in comparison to control group (p &lt; 0.001). After discontinuation of drug, histological evidence of recovery in the form of decrease in the thickness of capsule with decrease in subcapsular edema were observed in low dose groups on 40th and 60th sacrificial days as compared to high dose groups which showed late recovery on both time points (p &lt; 0.001).<br /><strong>Conclusion:</strong> The testicular capsular thickening induced by RBV is reversible after cessation of treatment.</p> <p>&nbsp;</p>

scholarly journals Effects of Experimental Hyperthyroidism on Collagen IV and Laminin-α5 Gene Expression in Balb/C Mouse Seminiferous Tubules

2019 ◽  
Vol 8 ◽  
Author(s):  
Saeed Sadeghi ◽  
Mahdi Jalali ◽  
Mohammad Reza Nikravesh ◽  
Mojtaba Sankian
Keyword(s):  
Gene Expression ◽  
Seminiferous Tubule ◽  
Evolutionary Process ◽  
Collagen Iv ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Weak Reaction ◽  
Type Iv ◽  
And Control ◽  
Experimental Group

Background: Hyperthyroidism is one of the disorders of the thyroid gland, an organ that controls the cellular and molecular behaviors of the seminiferous tubule basement membrane (BM), and ultimately, influences its evolutionary process. We aimed to investigate the effects of hyperthyroidism on immunohistochemical characteristics and gene expression levels of collagen IV and laminin-α5 in seminiferous tubules BM of Balb/C mice. Materials and Methods: Twenty male Balb/C mice were divided into experimental and control groups. The experimental group received 500 mg/l of levothyroxine (L-thyroxine) diluted in drinking water for two months to inducing hyperthyroidism, which was confirmed by radioimmunoassay. At the end of the study, the mice were sacrificed, and their testes were extracted for immunohistochemistry and real-time polymerase chain reaction assays. Results: Although a weak reaction was observed in the experimental specimens, no significant enhancement was noted in color intensity of type IV collagen in the seminiferous tubules BM of the experimental group as compared to the control group (P>0.05). Collagen IV gene expression results in the experimental group were not significantly different from the controls (P>0.05). Thus, there was a significant increase in laminin α5 gene expression compared to the control group (P=0.016). Conclusion: Considering the key role of collagen IV and laminin-α5 in the seminiferous tubule BM in the testes, the results of this study indicated that hyperthyroidism has important effects on both structures and functions of these two components. [GMJ.2019;8:e1369]

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