scholarly journals Effect of seed bacterization with plant growth-promoting bacteria on wheat productivity and phosphorus mobility in the rhizosphere

2019 ◽  
Vol 65 (No. 6) ◽  
pp. 313-319 ◽  
Author(s):  
Tatiana Arkhipova ◽  
Nailya Galimsyanova ◽  
Ludmila Kuzmina ◽  
Lidiya Vysotskaya ◽  
Ludmila Sidorova ◽  
...  
Keyword(s):  
Seed Treatment ◽  
Field Experiments ◽  
Plant Root ◽  
Bacterial Species ◽  
Wheat Crop ◽  
Plant Growth Promoting Bacteria ◽  
Maximum Increase ◽  
Wheat Roots ◽  
Auxin Content

Bacterization of the seeds of spring durum wheat with the strains of gram-positive aerobic spore-forming bacteria Bacillus subtilis IB-21 and B. subtilis IB-22 and gram-negative bacteria Advenella kashmirensis IB-К1and Pseudomonas extremaustralis IB-К13-1А was performed to study its effect on the productivity of plants, their hormonal content and rhizosphere phosphorus (P) status in the field experiments. A. kashmirensis IB-К1 andP. extremaustralis IB-К13-1А were the most capable of mobilizing hardly soluble phosphates in vitro, while P. extremaustralis IB-К13-1А produced the greatest concentration of auxins. All the studied strains successfully colonized the plant root system, the level of colonization detected during the second leaf stage being the highest in the case of A. kashmirensis IB-К1 and B. subtilis IB-22. Seed treatment with all the tested bacterial species resulted in an increase in phosphate mobility in the rhizosphere. Auxin content in wheat roots was increased by bacterization of seeds with P. extremaustralis IB-К13-1 and B. subtilis IB-22. The maximum increase in components of wheat crop yield (the mass of grains in the main and axillary spikes) was detected during 3 vegetative periods (2016, 2017 and 2018) in the case of seed treatment with the strains inducing a significant increase in auxin content in the roots of the treated plants related to either the highest bacterial capacity of producing this hormone in vitro (in the case of P. extremaustralis IB-К13-1А) or root colonization (in the case of B. subtilis IB-22).

scholarly journals Pathogenicity of Fusarium species associated with crown and root rot of wheat (Triticum aestivum) under different condition

2019 ◽  
Vol 7 (1) ◽  
pp. 1-9
Author(s):  
Bareen Sidqi Shareef Al-Tovi ◽  
Raed Abduljabbar Haleem
Keyword(s):  
Disease Severity ◽  
Root Rot ◽  
Field Experiments ◽  
Fusarium Species ◽  
Test Tube ◽  
Spore Suspension ◽  
Wheat Crop ◽  
Pathogenicity Test ◽  
Crown And Root Rot

This study was conducted to test the pathogenicity of Fusarium species, the causes of crown and root rot disease of wheat crop, under three different conditions (Laboratory, Greenhouse and Field) and to show the best method for pathogenicity among different conditions. Pathogenicity test of six isolates of Fusarium species (F. graminearum, F. oxysporum, F. avenaceum, F. nivale, F. solani and F. udum) was tested on durum (Simeto) cultivar of wheat by test tube method in the laboratory, the tested fungi had substantial effect on seed germination. F. oxysporum showed the highest germination failure (44.44%) which significantly differed with other species. In the greenhouse, seedlings were inoculated by spore suspension at the base of each plant stem. The most virulent fungus after 35 days of inoculation was F. oxysporum (0.78) followed by F. solani (0.70) and F. graminearum (0.66), while the lowest disease severity was recorded by F. udum (0.16). Also in the field pathogenicity experiments of three Fusarium species (F. graminearum, F. oxysporum and F. solani) were performed on a durum (Simeto) and soft (Cham6) cultivars. Spore suspension was applied at the 2- to 3-leaf Zadoks’s growth stage. Disease severity was calculated at two stages of wheat growth (Booting and Ripening).The most virulent fungus was F. graminearum (0.42) that was significantly different from  other fungi. This work indicated that F. graminearum, F. oxysporum and F. solani showed higher infection than remaining tested species under threeconditions. Pathogenicity test in laboratory by test tube method (In-vitro) appeared more effective than greenhouse and field experiments

scholarly journals Evaluation of Multiple Impacts of Furfural Acetone on Nematodes In Vitro and Control Efficiency against Root-Knot Nematodes in Pots and Fields

Antibiotics ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 605
Author(s):  
Wanli Cheng ◽  
Xue Yang ◽  
Li Zeng ◽  
Dian Huang ◽  
Minmin Cai ◽  
...  
Keyword(s):  
Field Experiments ◽  
Plant Root ◽  
Reduction Rate ◽  
Disease Index ◽  
Economic Losses ◽  
Egg Hatching ◽  
Root Knot Nematodes ◽  
Control Efficiency ◽  
The Impact

Root-knot nematodes (RKNs) seriously endanger agricultural development and cause great economic losses worldwide. Natural product furfural acetone (FAc) is a promising nematicide with strong attractant and nematicidal activities, but baseline information about the impact of FAc on the reproduction, egg hatching, feeding, and growth of nematodes and its pest control efficiency in field are lacking. Here, the inhibition effects of FAc on nematodes in vitro and its RKN control efficiency in pot and field were investigated. FAc inhibited the egg hatching of Meloidogyne incognita by 91.7% at 200 mg/L after 2 days and suppressed the reproduction, feeding, and growth of Caenorhabditis elegans in vitro. In pot experiments, FAc in various dosages reduced the disease index of plant root significantly. In field experiments, FAc exhibited control effect on RKNs equivalent to commercial nematicides avermectin and metam sodium, with a reduction in disease index by 36.9% at a dose of 50 mg/plant. FAc also reduced the population density of RKNs in soil, with a reduction rate of 75.3% at the dose of 750 mg/m2. No adverse effect was detected on plant growth after FAc application. These results provide compelling evidence for development of FAc as an appropriate alternative for current nematicides.

scholarly journals Use of Trichoderma in Biological Control of Collar Rot of Soybean and Chickpea

2020 ◽  
pp. 25-31
Author(s):  
Mahbuba Kaniz Hasna ◽  
Md. Abul Kashem ◽  
Farid Ahmed
Keyword(s):  
Seed Treatment ◽  
Field Experiments ◽  
Disease Incidence ◽  
Sclerotium Rolfsii ◽  
Antagonistic Activity ◽  
Root Weight ◽  
Dual Culture ◽  
Collar Rot ◽  
Rot Disease

An in vitro and field experiments for two consecutive years were conducted at Bangladesh Institute of Nuclear Agriculture, Mymensingh, aiming to investigate the efficacy of Trichoderma harzianum against Sclerotium rolfsii causing collar rot disease of soybean and chickpea. In in vitro the antagonistic activity of T. harzianum against S. rolfsii was observed through dual culture. In field experiment Trichoderma was applied as soil treatment and seed treatment. The percent inhibition of S. rolfsii induced by T. harzianum was found upto 78.9% in in vitro. The maximum reduction of collar rot disease incidence over control was 82.4% in soybean and 77.6% in chickpea which was recorded in the plot where T. harzianum was applied in the soil. The highest seed germination: 86.3% in soybean and 84.8% in chickpea, maximum fresh shoot weight: 94.5 g plant-1 in soybean, 62.5 g plant-1 in chickpea, maximum fresh root weight: 10.7 g plant-1 in soybean, 9.3 g plant-1 in chickpea and the highest yield: 2830 kg ha-1 in soybean, 1836 kg ha-1 in chickpea were obtained by the application of Trichoderma in soil. The study indicated that the tested isolate of T. harzianum had potential in controlling collar rot disease of soybean and chickpea. For the reduction of collar rot incidence application of T. harzianum in soil was found more effective than seed treatment. 

Response of a Chlorsulfuron-Resistant Biotype ofKochia scopariato Sulfonylurea and Alternative Herbicides

Weed Science ◽  
1993 ◽  
Vol 41 (1) ◽  
pp. 100-106 ◽  
Author(s):  
Lyle F. Friesen ◽  
Ian N. Morrison ◽  
Abdur Rashid ◽  
Malcolm D. Devine
Keyword(s):  
Field Experiments ◽  
Good Control ◽  
Acetolactate Synthase ◽  
In Vitro Assays ◽  
Shoot Biomass ◽  
Wheat Crop ◽  
Enzyme Assays ◽  
Dry Matter Accumulation ◽  
Severe Stunting

Kochia growing on an industrial site where chlorsulfuron was applied repeatedly over several seasons was confirmed to be resistant to chlorsulfuron and several other acetolactate synthase (ALS) -inhibiting herbicides. In growth room experiments, resistant (R) plants were 2 to >180 times more resistant to five sulfonylurea herbicides and one imidazolinone herbicide (imazethapyr) than susceptible (S) plants, as measured by the ratio of dosages required to inhibit shoot dry matter accumulation by 50% (GR50R/S). Similarly, in vitro assays of ALS activity indicated that from 3 to 30 times more herbicide was required to inhibit the enzyme from R plants than from S plants. Results of ALS enzyme assays indicated that R kochia was approximately equally resistant to metsulfuron, triasulfuron, and thifensulfuron, and 2.5 times more resistant to tribenuron than thifensulfuron. However, the response of R kochia growing in a spring wheat crop in the field was not consistent with results of the ALS enzyme assays. In field experiments, thifensulfuron at 32 g ai ha−1had little effect on R kochia. In contrast, metsulfuron, triasulfuron, and tribenuron at 8 g ha−1did not reduce R kochia seedling densities, but caused severe stunting such that 2 mo after treatment the shoot biomass of plants in untreated plots was four times greater than in sprayed plots. Herbicides with alternative modes of action including fluroxypyr, bromoxynil/MCPA ester, dichlorprop/2,4-D ester, and 2,4-D ester provided good control of R kochia in the field. Quinclorac did not reduce kochia densities, but surviving plants were stunted. To delay or avoid development of ALS inhibitor-resistant kochia populations, these alternative herbicides applied alone or in tank mixtures could be incorporated into a herbicide rotation.

scholarly journals Evaluation of the New Compound Oxathiapiprolin for Control of Downy Mildew in Basil

2015 ◽  
Vol 16 (4) ◽  
pp. 165-172 ◽  
Author(s):  
Jaimin S. Patel ◽  
Maria I. Costa de Novaes ◽  
Shouan Zhang
Keyword(s):  
Downy Mildew ◽  
Seed Treatment ◽  
Field Experiments ◽  
Foliar Application ◽  
Tea Tree Oil ◽  
Baseline Sensitivity ◽  
Tea Tree ◽  
In Vitro Sensitivity ◽  
Potassium Phosphite

This study was conducted to evaluate a new chemical, oxathiapiprolin, as a seed treatment and foliar application for control of downy mildew of basil, caused by Peronospora belbahrii, and to assess the in vitro sensitivity of P. belbahrii to oxathiapiprolin. In the greenhouse, both foliar application and seed treatment with oxathiapiprolin significantly (P < 0.05) reduced downy mildew severity on basil and increased width of cotyledons compared to the non-treated control. In field experiments, foliar applications of oxathiapiprolin alone, as a tank mixture, or in alternation with azoxystrobin, potassium phosphite, or tea tree oil significantly reduced downy mildew severity and increased the basil yield compared to the non-treated control. In the laboratory test, EC50 values of sensitive P. belbahrii isolates ranged from 0.00033 μg/ml to 0.00040 μg/ml oxathiapiprolin, providing important information on baseline sensitivity of P. belbahrii to oxathiapiprolin. The results indicate that oxathiapiprolin can be applied as foliar applications in combination or in alternation with azoxystrobin or potassium phosphite to control the disease effectively. Accepted for publication 1 November 2015. Published 13 November 2015.

scholarly journals Evaluation of plant growth-promoting and bioprotecting rhizobacteria on wheat crop

2001 ◽  
Vol 26 (3) ◽  
pp. 597-600 ◽  
Author(s):  
WILMAR C. da LUZ
Keyword(s):  
Plant Growth ◽  
Chemical Treatment ◽  
Seed Treatment ◽  
Field Experiments ◽  
Seedling Emergence ◽  
Wheat Crop ◽  
Chemical Treatments ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Biotype B

Experiments were carried out under laboratory, growth chamber, and field conditions to evaluate the effect of Plant growth-promoting and bioprotecting rhizobacteria (PGPBR) seed treatment on seed pathogens, seed germination, plant growth, and grain yield of wheat (Triticum aestivum). Most of the PGPBR strongly reduced the recovery of the pathogens from infected wheat seeds. All treatments, except the chemical iprodione + thiram, significantly promoted plant growth over the nontreated control. Psudomonas putida biotype A (11) and P. agglomerans (14) showed the greatest effects. Field experiments, carried out at two locations, indicated that all treatments, except P. chlororaphis (42), significantly increased seedling emergence of wheat . In Pato Branco, PR, P. putida biotype A (11) and P. putida biotype B (44) presented the best results, both being superior to fungal biological and chemical treatments. In Passo Fundo P. putida biotype A (11) and P. putida biotype B (17 and 44) significantly improved yield over the nontreated control. Yield increases of these three PGPBR were similar to the chemical treatment iprodione + thiram. In Pato Branco, P. putida biotype A (11) and P. putida biotype B (17), as well as the chemical treatment, provided significant increase over the nontreated control. Yield increases by the PGPBR varied from 18% to 22% in Passo Fundo and from 27% to 28% in Pato Branco.

Evaluation of Suitable IPM Module for Management of YMV Disease in Mungbean under West Central Table Land Zone of Odisha

2021 ◽  
Author(s):  
Dipankar Mandal ◽  
Rini Pal ◽  
Atanu Seni ◽  
Ashok K. Mohanty
Keyword(s):  
Seed Treatment ◽  
Field Experiments ◽  
Cost Benefit ◽  
Pooled Analysis ◽  
Insect Vector ◽  
Maximum Increase ◽  
Vector Management ◽  
Transfer Station ◽  
Cost Benefit Ratio ◽  
Yellow Sticky Trap

Background: YMV disease in mungbean is one of the major disease causing heavy losses annually throughout the country. As the disease is transmitted by insect vector, management of vector is important to check the YMV disease that can minimise the losses. The present investigation was aimed to evaluate different IPM modules for management of YMV disease of mungbean. Methods: Field experiments were carried out during Rabi season of 2016-17 and 2017-18 at the Research Farm of Regional Research and Technology Transfer Station, Chiplima, Sambalpur, Odisha, India. Population of whitefly was recorded on three leaves selected from top, middle and bottom canopy of the plant. Disease severity was recorded by using 0-9 scale. Result: The IPM module i.e. seed treatment with Thiamethoxam 25 WG @ 5 gm kg-1 of seed, installation of yellow sticky trap @ 50 ha-1 and spraying of Acetamiprid 20 SP @ 0.3 gm l-1 of water was found as the most effective among all other modules. Pooled analysis of two years data revealed that 65.5% YMV disease control and 59.3% reduction of white fly population over control were performed by the said IPM module. A maximum increase in yield (84.8%) and highest cost benefit ratio (1.77) were also achieved with the module.

Production of Novel Bioactive Compounds by the Bacteria Associated with Some Marine Sponges of Gulf of Mannar and Palk Bay, Southeast Coast of India

2018 ◽  
Vol 6 (8) ◽  
pp. 183
Author(s):  
V. Ramadas ◽  
G. Chandralega
Keyword(s):  
Bioactive Compounds ◽  
Bacterial Species ◽  
Marine Sponges ◽  
Marine Organisms ◽  
Gulf Of Mannar ◽  
Bacterial Symbionts ◽  
Palk Bay ◽  
Excellent Source

Sponges, exclusively are aquatic and mostly marine, are found from the deepest oceans to the edge of the sea. There are approximately 15,000 species of sponges in the world, of which, 150 occur in freshwater, but only about 17 are of commercial value. A total of 486 species of sponges have been identified in India. In the Gulf of Mannar and Palk Bay a maximum of 319 species of sponges have been recorded. It has been proved that marine organisms are excellent source of bioactive secondary metabolites and number of compounds of originated from marine organisms had been reported to possess in-vitro and in-vivo immuno stimulatory activity. Extracts from 20 sponge species were tested for bacterial symbionts and bioactive compounds were isolated from such associated bacterial species in the present study.

Characterization of the Reproductive Mode in Guayule In Vitro

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 483a-483
Author(s):  
Roy N. Keys ◽  
Dennis T. Ray ◽  
David A. Dierig
Keyword(s):  
Field Experiments ◽  
Reproductive Mode ◽  
Dichlorophenoxyacetic Acid ◽  
Initial Field ◽  
Breeding Lines ◽  
Reproductive Modes ◽  
Desert Southwest ◽  
2,4 Dichlorophenoxyacetic Acid

Guayule (Parthenium argentatum Gray, Asteraceae) is a latex-producing perennial desert shrub that is potentially of economic importance as an industrial crop for the desert Southwest. It is known to possess complex reproductive modes. Diploids are predominantly sexual and self-incompatible, while polyploids show a range of apomictic potential and self-compatibility. This paper describes the development of a relatively rapid and simple technique for characterizing reproductive modes of breeding lines of P. argentatum. Initial field experiments were based on an auxin test used successfully to characterize reproductive mode in the Poaceae. The application of 2,4-dichlorophenoxyacetic acid inhibited embryo formation in P. argentatum, but this was not the case with other auxins tested. Results of field experiments were ambiguous because: 1) the floral structure of P. argentatum is such that auxins might not have penetrated to the ovules, and 2) there was potential self-fertilization by pollen released within isolation bags. Therefore, in vitro culture of flower heads was tested because it provided much better control of environmental conditions, growth regulator application, and pollen release. Auxin alone, or in combination with gibberellic acid or kinetin, inhibited parthenogenesis in vitro. Embryo production did not vary using two substantially different nutrient media. In vitro flower head culture using a (Nitsch and Nitsch) liquid nutrient medium without growth regulators, enabled characterization of the reproductive mode of seven breeding lines, ranging from predominantly sexual to predominantly apomictic. The results of this technique were substantiated using RAPD analyzes of progeny arrays from controlled crosses.

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