scholarly journals Epidemiology of Theileria equi in Persian Arab horses from Iran

2014 ◽  
Vol 59 (No. 9) ◽  
pp. 409-414 ◽  
Author(s):  
S. Bahrami ◽  
AR Ghadrdan ◽  
M. Pourmahdi Borujeni ◽  
M. Vafayi Salarpur
Keyword(s):  
Geographical Variation ◽  
Regulatory Agencies ◽  
Theileria Equi ◽  
Blood Samples ◽  
Khuzestan Province ◽  
Horse Breeding ◽  
Pcr Method ◽  
Horse Industry ◽  
And Training ◽  
Positive Results

The Khuzestan province in south-western Iran is the centre of Persian Arab horse breeding and training. The present study was aimed at determining the prevalence of Theileria equi in the equids of this province. A total of 165 blood samples from healthy Persian Arab horses from twenty four stables were examined for the presence of T. equi infection using molecular methods. For detection of T. equi, primers targeting the 18SrRNA gene were selected. The PCR method gave 47 (28.5%) positive results. Age (P = 0.68), sex (P = 0.88), contact with cattle (P = 0.26) and type of activity (P = 0.06) were not determined as risk factors for T. equi infection in this study. However, there was a significant geographical variation in the prevalence of T. equi infection ranging from 8.3% (2/24) in Shushtar to 55.6% (10/18) in Ramhormoz (CI, 2.46–76.82) (P = 0.003). In conclusion, equine theileriosis has the potential of posing a significant problem for Iran’s Persian Arab horse industry and should remain a major concern to the horse community and regulatory agencies.  

Diagnosis of helicobacter pylori infection: problems and prospects

2020 ◽  
pp. 54-59
Author(s):  
A. S. Molostova ◽  
N. S. Gladyshev ◽  
A. V. Svarval ◽  
R. S. Ferman ◽  
A. B. Karasyova ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Urease Activity ◽  
Diagnostic Methods ◽  
Study Group ◽  
Biochemical Method ◽  
Antimicrobial Drugs ◽  
Non Invasive ◽  
Number Of Patients ◽  
Pcr Method ◽  
Positive Results

(HP) infection was performed using invasive and non-invasive methods. The study group consisted of 95 patients with dyspepsia. HP infection was detected in 47 patients (49.4 %). The expediency of using a set of diagnostic methods for detecting HP (PCR, immunochromatographic, bacteriological and method for determining urease activity) is proved. Most often (100 %) in patients HP infection was detected in biopsies using the PCR method. Somewhat less frequently it was detected when examining biopsies with an invasive biochemical method (AMA RUT Reader) (82 %) and fecal immunochromatographic method (83 %). Despite the fact that helicobacteriosis was detected bacteriologically in a small number of patients (24 %), this method is of particular value, since it allows you to assess the sensitivity to antimicrobial drugs and probiotics, and does not give false positive results.

Prevalence of SHV-extended spectrum β-lactamase producing carbapenem –resistantKlebsiellapneumoniae among patients with lower respiratory tractinfections in Babylon Province-Iraq.

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Fatima Moeen Abbas
Keyword(s):  
Resistance Rate ◽  
Combination Method ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Carbapenem Resistant ◽  
Extended Spectrum ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Tract Infections ◽  
Positive Results

This study was carried out to screen the prevalence of Klebsiella pneumoniae isolated from patients with lower respiratory tract infections in Babylon province.From December,2015 to the end of March,2016,a total of 100 sputum samples were collected from patients visited or hospitalized Merjan Teaching Hospital and Al- Hashimya General Hospital. Fifteenth (65%) isolates were identified as Klebsiellapneumoniae. All bacterial isolates were evaluated for extended spectrum β-lactamase (ESBL) production phenotypically using disk combination method. Eleven (73.3%) isolates were detected as ESBL-producers. Kirby-Bauer disk diffusion method was employed to determine resistance profile of ESBLs-positive isolates. Higher rates of resistance were observed for ampicillin and piperacillin antibiotics with (81.8%) and (72.7%) resistance rate, respectively, while the lowest rate was noticed for imipenem antibiotic (14.28%). Carbapenem-resistant isolates were investigated for blaSHV gene by Polymerase Chain Reaction (PCR) method, 2 (100%) isolates gave positive results.

scholarly journals Apicomplexan Protozoa Responsible for Reproductive Disorders: Occurrence of DNA in Blood and Milk of Donkeys (Equus asinus) and Minireview of the Related Literature

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 111
Author(s):  
Stefania Perrucci ◽  
Lisa Guardone ◽  
Iolanda Altomonte ◽  
Federica Salari ◽  
Simona Nardoni ◽  
...  
Keyword(s):  
Animal Species ◽  
Neospora Caninum ◽  
Reproductive Disorders ◽  
Theileria Equi ◽  
Blood Samples ◽  
Related Literature ◽  
Milk Samples ◽  
Equus Asinus ◽  
Time Periods ◽  
Blood Specimens

Donkeys may be susceptible to many pathological agents and may act as carriers of pathogens for other animal species and humans. This study evaluated the occurrence of potentially abortifacient apicomplexan protozoa DNA in blood and milk samples collected at different time periods during lactation (1, 6, and 10 months) from 33 healthy dairy jennies. A total of 73 blood and 73 milk samples were used for DNA extraction and analysis. Blood specimens from 11/33 (33%) jennies scored positive for Theileria equi, while milk samples scored negative. Blood and milk of 3/33 jennies yielded DNA of Toxoplasma gondii at 6 months (n. 1) and 10 months (n. 2) after parturition. Neospora caninum DNA was found in four milk and in five blood samples only at one month after parturition. This study is the first report about the presence of N. caninum DNA in milk of naturally infected jennies. Moreover, the excretion of N. caninum DNA in some of these jennies at 30 days from the parturition may suggest a possible occurrence of an endogenous cycle, while the presence of T. gondii DNA in the milk collected at 6 and 10 months after parturition may be suggestive of a discontinuous excretion.

Identification of Bacteroides forsythus in Subgingival Dental Plaque with the Aid of a Rapid PCR Method

1997 ◽  
Vol 76 (7) ◽  
pp. 1376-1380 ◽  
Author(s):  
J.H. Meurman ◽  
J. Wahlfors ◽  
A. Korhonen ◽  
P. Alakuijala ◽  
P. Väisänen ◽  
...  
Keyword(s):  
Dental Plaque ◽  
Subgingival Plaque ◽  
Chain Reaction ◽  
Rapid Pcr ◽  
Microbiological Methods ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Pre Treatment ◽  
Culture Positive ◽  
Positive Results

Bacteroides forsythus has been shown to be prevalent among patients with periodontitis. Conventional microbiological methods used to identify this bacterium, however, are laborious and time-consuming and are therefore not well-suited for screening purposes. We have developed a polymerase chain-reaction (PCR) method which is rapid, specific, and simple to perform and does not require other sample pre-treatment except a brief centrifugation. This method was applied to the detection of B. forsythus in subgingival plaque of 58 periodontitis patients. When compared with the results of conventional culturing, the PCR method always confirmed the culture-positive results, while none of the PCR negative samples was shown to be culture-positive. The PCR method appeared to give more than double the number of samples positive for B. forsythus than culturing (89.7% vs. 37.9%). The analysis requires less than 4 hrs to perform, and is specific only to B. forsythus and sensitive enough to detect fewer than 5 bacteria.

scholarly journals Seroprevalence of Hepatitis E Virus Infection among Blood Donors in Bulgaria

Viruses ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 492
Author(s):  
Magdalena Baymakova ◽  
Krasimira Terzieva ◽  
Rumen Popov ◽  
Elisaveta Grancharova ◽  
Todor Kundurzhiev ◽  
...  
Keyword(s):  
Virus Infection ◽  
Hepatitis E Virus ◽  
Blood Donors ◽  
Hepatitis E ◽  
Elisa Test ◽  
Blood Samples ◽  
Wild Boars ◽  
Domestic Pigs ◽  
Specific Questionnaire ◽  
Positive Results

Hepatitis E virus (HEV) infection is widespread among domestic pigs, industrial swine, and wild boars in Bulgaria. The aim of the current research was to present the HEV seroprevalence among blood donors in Bulgaria. In the present study, 555 blood donors (479 males and 76 females) were enrolled from five districts in the country (Shumen, Pleven, Stara Zagora, Plovdiv, and Sofia districts). All blood samples were tested for anti-HEV IgG using the recomWell HEV IgG ELISA test (Mikrogen GmbH, Neuried, Germany). Each participating donor completed a short, structured, and specific questionnaire to document data on the current study. Anti-HEV IgG positive results were detected in 144 (25.9%) blood donors, including 129 (26.9%) males and 15 (19.7%) females. The established HEV seropositivity was 28.8% (23/80) in Shumen district, 23.2% (22/95) in Pleven district, 27.1% (38/140) in Stara Zagora district, 27.5% (44/160) in Plovdiv district, and 21.3% (17/80) in Sofia district. A high HEV seroprevalence was found for persons who declared that they were general hunters (48.7%; 19/39; p = 0.001) and hunters of wild boars (51.6%; 16/31; p = 0.001). We present the first seroprevalence rates of HEV infection in blood donors from Bulgaria. The results of our research showed high HEV seropositivity among blood donors.

scholarly journals Theoretical Justification of the Meaning and Specifications of the Linguistic Competence in NGU Officers

2020 ◽  
pp. 35-50
Author(s):  
Volodymyr Liutyi ◽  
Keyword(s):  
Law Enforcement ◽  
Foreign Language ◽  
National Guard ◽  
Law Enforcement Officers ◽  
Armed Forces ◽  
Professional Activity ◽  
Language Competence ◽  
Security Forces ◽  
And Training ◽  
Positive Results

The relevance of the article is due to the European integration aspirations of Ukraine and the prospects of joining its security forces, including the National Guard of Ukraine, to the NATO. So, the formation of language competence in modern conditions is an integral part of the officer’s professional development and training. Theoretical analysis of scientific sources on the language competence development in servicemen of the Armed Forces of Ukraine and law enforcement officers of the Ministry of Internal Affairs of Ukraine revealed that this problem has not been studied and needs additional research. The essence of the language competence in the NGU officer is revealed as a personal integrative formation, which allows the specialist (an officer) effectively to communicate in foreign language, to achieve positive results in his professional activity. It is studied that the requirements of the NATO STANAG-6001, as well as various aspects of the NGU's professional activity should be taken into account when developing the language competence in NGU officers.

scholarly journals Development of multiplex real-time RT-PCR assay for the detection of SARS-CoV-2

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0250942
Author(s):  
Huseyin Tombuloglu ◽  
Hussein Sabit ◽  
Ebtesam Al-Suhaimi ◽  
Reem Al Jindan ◽  
Khaled R. Alkharsah
Keyword(s):  
Real Time ◽  
Single Gene ◽  
False Negative ◽  
Diagnostic System ◽  
Virus Detection ◽  
Current Method ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Positive Results ◽  
Good Agreement

The outbreak of the new human coronavirus SARS-CoV-2 (also known as 2019-nCoV) continues to increase globally. The real-time reverse transcription polymerase chain reaction (rRT-PCR) is the most used technique in virus detection. However, possible false-negative and false-positive results produce misleading consequences, making it necessary to improve existing methods. Here, we developed a multiplex rRT-PCR diagnostic method, which targets two viral genes (RdRP and E) and one human gene (RP) simultaneously. The reaction was tested by using pseudoviral RNA and human target mRNA sequences as a template. Also, the protocol was validated by using 14 clinical SARS-CoV-2 positive samples. The results are in good agreement with the CDC authorized Cepheid`s Xpert® Xpress SARS-CoV-2 diagnostic system (100%). Unlike single gene targeting strategies, the current method provides the amplification of two viral regions in the same PCR reaction. Therefore, an accurate SARS-CoV-2 diagnostic assay was provided, which allows testing of 91 samples in 96-well plates in per run. Thanks to this strategy, fast, reliable, and easy-to-use rRT-PCR method is obtained to diagnose SARS-CoV-2.

scholarly journals Short Communication: Molecular characterization and blood hematology profile of dogs infected by Ehrlichia canis in Yogyakarta, Indonesia

2020 ◽  
Vol 21 (7) ◽  
Author(s):  
Yustinus oswin primajuni Wuhan ◽  
Aris Haryanto ◽  
Ida Tjahajati
Keyword(s):  
Molecular Characterization ◽  
Microscopic Examination ◽  
Short Communication ◽  
Pcr Amplification ◽  
Ehrlichia Canis ◽  
Infected Blood ◽  
Blood Samples ◽  
Glta Gene ◽  
Vector Borne ◽  
Positive Results

Abstract. Wuhan YOP, Haryanto A, Tjahajati I. 2020. Short Communication: Molecular characterization and blood hematology profile of dogs infected by Ehrlichia canis in Yogyakarta, Indonesia. Biodiversitas 21: 3242-3248. Ehrlichia canis is Gram-negative intracellular obligate bacteria that cause ehrlichiosis, a companion vector-borne disease is a potentially fatal disease that attacks dogs. The purpose of this study was to molecular characterize and determine the features of Ehrlichia-infected blood based on the amplification of the gltA gene in Ehrlichia infected dogs from Yogyakarta, Indonesia. Blood samples were collected from 51 dog patients from the Prof. Dr. Soeparwi Animal Hospital, animal clinics, and pet shops based on the anamnesis, clinical sign, and physical examination, followed by microscopic examination, routine hematology, PCR amplification, and DNA sequencing were carried out on the blood samples. Based on positive PCR amplification and blood hematology profile examination ehrlichiosis-positive in dogs showed that thrombocytopenia case was 82.3%, anemia was 70.5%, eosinopenia was 70.5%, neutropenia was 29.4%, monocytopenia was 23%, leukopenia was 17% and lymphopenia was 11.7%. Morulae of Ehrlichia sp.was not found in microscopic examination. Molecularly, detected of E. canis using the gltA gene showed that 34% of samples were positive results. Then 5 of positive Ehrlichia samples were DNA sequenced, they showed a high homology of 100% with Hat Yai isolates (KU765199.1). There was no genetic diversity between E. canis samples in Yogyakarta.

scholarly journals Come from away: Best practices in mini-sabbaticals for the development of young investigators: a White Paper by the SEQUIN (mini-Sabbatical Evaluation and QUality ImprovemeNt) Group

2019 ◽  
Vol 3 (1) ◽  
pp. 37-44 ◽  
Author(s):  
Michael H. Pillinger ◽  
Stephenie C. Lemon ◽  
Martin S. Zand ◽  
P. Jeffrey Foster ◽  
Jeanne S. Merchant ◽  
...  
Keyword(s):  
Best Practice ◽  
Research Unit ◽  
White Paper ◽  
National Institutes Of Health ◽  
Regulatory Agencies ◽  
Limited Data ◽  
Non Profit ◽  
History Of ◽  
Training Institutions ◽  
And Training

AbstractMini-sabbaticals are formal short-term training and educational experiences away from an investigator’s home research unit. These may include rotations with other research units and externships at government research or regulatory agencies, industry and non-profit programs, and training and/or intensive educational programs. The National Institutes of Health have been encouraging training institutions to consider offering mini-sabbaticals, but given the newness of the concept, limited data are available to guide the implementation of mini-sabbatical programs. In this paper, we review the history of sabbaticals and mini-sabbaticals, report the results of surveys we performed to ascertain the use of mini-sabbaticals at Clinical and Translational Science Award hubs, and consider best practice recommendations for institutions seeking to establish formal mini-sabbatical programs.

scholarly journals Effects of Sociality Level on Companion Dog Training through Food Reinforcement

Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2413
Author(s):  
Ok-Deuk Kang
Keyword(s):  
Contact Time ◽  
Food Reinforcement ◽  
Active Phase ◽  
Companion Dogs ◽  
Dog Training ◽  
First Contact ◽  
And Training ◽  
Positive Results

The purpose of this study was to investigate the effect of sociality level in dogs using food reinforcement. The companion dogs living mostly inside (IS) and those living mostly outside (OS) groups were further classified into high sociality (HS) and low sociality (LS) groups using a social test. The data were analyzed by observing videos of the dogs and recording nine categories of sociality. Passive; activity; and communication tasks were measured in terms of the time the dog first contacted the trainer; the time it took for the dog to approach within 1 m of the trainer, and the time the dog remained in contact. The IS and OS groups showed no significant differences in any category; except for close to trainer within 1 m of the active phase. However, in a comparison between the overall HS and LS groups; significant positive changes were observed in all items except for the first contact time to the trainer of the passive and active phase. This is an important result of the sociality level; as positive results can be predicted not only in a dog’s ability to adapt to the environment but also in the communication and training exchanges between dogs and humans

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