The effectiveness of combined therapy of dyslipidemia by atorvastatin and biologycally active substances from marine hydrobionts

Introduction. The perspective direction in therapy of dyslipidemia (DLP) is a complex application of biologycally active substances from marine hydrobionts. Objective. The purpose of research - to compare the lipidcorrective action of preparates based on biologically active substances from marine hydrobionts - fukolam and maristim - individually and in combination with atorvastatin in patients with DLP. Materials and methods. 250patients with DLP and 40 healthy donors were included in research. As medicines atorvastatin, fukolam, maristim were used. In the blood serum the levels of lipid spectrum were determined. Results. Hypolipidemic action of fukolam in complex of basic therapy of patients with DLP was implemented by gradually reducing the total cholesterol, cholesterol of low-density lipoproteins, triglycerides levels to control values. The efficacy of fukolam was comparable with that of atorvastatin in a daily dose of 10 mg. Complex of «fukolam-maristim» showed hypolipidemic effect in severe hyperlipidemia. Conclusions. Combined therapy with use of bioactive substances of natural origin may be one of the way to improve the efficiency of hypolipidemic therapy. We have developed the algorithm of differential correction of DLP by inclusion the fukolam and maristim in complex therapy.

Download Full-text

COMPARATIVE ANALYSIS OF BIOACTIVE SUBSTANCES CONTENT IN THE LEAVES AND FRUITS OF A ROWAN-TREE (SORBUS AUCUPARIA FRUCTUS) AND ASSESSMENT OF PROSPECTS IN MEDICINE AND PHARMACY

Global problems of modernity ◽

10.26787/nydha-2713-2048-2020-1-10-11-12-58-62 ◽

2020 ◽

pp. 58-62

Author(s):

Chernichkina A.D.

Keyword(s):

Raw Materials ◽

Wide Distribution ◽

Biologically Active ◽

Bioactive Substances ◽

Plant Raw Materials ◽

Mountain Ash ◽

The Russian Federation ◽

Materials Used ◽

Active Substances ◽

Pectin Substances

A large number of biologically active substances, organic acids, tannins, and pectin substances were detected during the study of fruits, leaves, and pulp of the fruits of mountain Ash. The content of these substances in leaves and pulp will significantly expand the arsenal of medicinal plant raw materials used. Given the wide distribution of mountain Ash in the territory of the Russian Federation, harvesting leaves from the same plants after harvesting the fruit and using pulp will make it possible to obtain new phytopreparations.

Download Full-text

Biologically Active Constituents of Leaves and Roots of Aloe arborescens var. natalensis

Zeitschrift für Naturforschung C ◽

10.1515/znc-1977-9-1010 ◽

1977 ◽

Vol 32 (9-10) ◽

pp. 731-734 ◽

Cited By ~ 34

Author(s):

Toshifumi Hirata ◽

Takayuki Suga

Keyword(s):

Succinic Acid ◽

Inhibitory Action ◽

Biologically Active ◽

Bioactive Substances ◽

Active Constituents ◽

Gastric Juice Secretion ◽

Aloe Arborescens ◽

Aloe Emodin ◽

Active Substances ◽

Leaf Juice

Abstract Several biologically active substances, such as aloenin (1), magnesium lactate, aloe-emodin (4), barbaloin (5), and succinic acid, were found to be contained in the leaf juice of Aloe arborescens Mill. var. natalensis Berger, which has widely been used in domestic medicines. Aloenin (1) and magnesium lactate were elucidated to exhibit an inhibitory action on the gastric juice secretion of rats. Various constituents other than the above bioactive substances were found in the leaves and the roots of the plant.

Download Full-text

Study of the Properties of In Vitro Dactylorhiza maculata (L.) Soó (Family Orchidaceae) Extracts

Plants ◽

10.3390/plants10071330 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1330

Author(s):

Stanislav Sukhikh ◽

Svetlana Noskova ◽

Svetlana Ivanova ◽

Liubov Skrypnik ◽

Artem Pungin ◽

...

Keyword(s):

Antioxidant Properties ◽

Antimicrobial Properties ◽

Feed Additives ◽

Water Soluble ◽

Biologically Active Substances ◽

Biologically Active ◽

Bioactive Substances ◽

Limit Values ◽

Active Substances

The medicinal plant Dactylorhiza maculata (L.) Soó (family Orchidaceae) is used to treat gastritis, colic, gastrointestinal tract, and bladder diseases. This study aimed to investigate the properties and characteristics of the in vitro Dactylorhiza maculata extract. The recommended parameters for producing Dactylorhiza maculata extract were determined: temperature 60 °C, process duration 60 min, hydro module 1:10. It is recommended to carry out the extraction using an aqueous ethyl alcohol solution with a mass fraction of the parent substance of 70%. It was found that such biologically active substances as rutin, quercetin, 3,3’,4’,5,5’,7-hexahydroxyflavonone, 3,3’,4’,5,5’,7-hexahydroxyflavonone-3-O-glycoside, gallic acid, and ferulic acid were dominant in Dactylorhiza maculata. A high phosphorus content was noted (2410.8 mg/kg dry matter). The studied Dactylorhiza maculata samples contained a large number of organic acids and water-soluble vitamins. The tested extracts were safe in terms of the content of heavy metals, pesticides, aflatoxin B1, and radionuclides, as well as pathogenic and opportunistic microorganisms; the content was significantly lower than the threshold limit values. The studied complex of biologically active substances from Dactylorhiza maculata extract samples had antimicrobial properties. It was found that the antioxidant activity of the samples was 217.89 ± 10.89 mg AA/g (AA—ascorbic acid). The high content of bioactive substances and the antimicrobial and antioxidant properties of Dactylorhiza maculata extract samples determine the application potential of this plant as a substitute for growth stimulants and feed antibiotics in the production of feed additives, aiming to increase the physiological and immune status of livestock and poultry.

Download Full-text

Isothiazoles in the Design and Synthesis of Biologically Active Substances and Ligands for Metal Complexes

Synthesis ◽

10.1055/s-0039-1690688 ◽

2019 ◽

Vol 52 (02) ◽

pp. 159-188 ◽

Cited By ~ 7

Author(s):

Alexey V. Kletskov ◽

Nikolay A. Bumagin ◽

Fedor I. Zubkov ◽

Dmitry G. Grudinin ◽

Vladimir I. Potkin

Keyword(s):

Metal Complexes ◽

Plant Protection ◽

Cross Coupling ◽

Biologically Active Substances ◽

Biologically Active ◽

New Drugs ◽

Bioactive Substances ◽

High Biological Activity ◽

Wide Range ◽

Active Substances

The chemistry of isothiazoles is being intensively developed, which is evidenced by the wide range of selective transformations involving the isothiazole heterocycle and the high biological activity of its derivatives that can be used as effective new drugs and plant protection chemicals. Some representatives of isothiazoles have proven to be synergists of bioactive substances, which opens the way to lower the doses of drugs used and is especially important in cancer chemotherapy. In the framework of the present review, the accomplishments in the chemistry of isothiazoles over the past 18 years are examined, whilst current strategies for the synthesis of isothiazole-containing molecules and key directions of studies in this field of heterocyclic chemistry are discussed. Considerable attention is paid to chlorinated isothiazoles and strategies for their use in the synthesis of biologically active substances. In addition, a comprehensive review of existing literature in the field of metal complexes of isothiazoles is given, including the results and prospects for the practical use of isothiazole–metal complexes as catalysts for cross-coupling reactions in aqueous and aqueous–alcoholic media (‘green chemistry’).1 Introduction2 Synthesis by Ring-Forming Reactions2.1 Intramolecular Cyclization2.2 (4+1)-Heterocyclization2.3 (3+2)-Heterocyclization2.4 Syntheses by Ring Transformations3 Isothiazoles by Ring Functionalization Reactions: Nucleophilic Substitution, Cross-Coupling and Side-Chain Functionalization4 Selected Syntheses of Biologically Active Isothiazole Derivatives5 Isothiazoles in the Synthesis of Transition-Metal Complexes and in Metal-Complex Catalysis6 Conclusion

Download Full-text

Standardization of Dry Extract of Pumpkin Pulp Juice, Which Has a Hypolipidemic Effect on the Content of Β-Carotene

Medicina ◽

10.29234/2308-9113-2021-9-1-79-92 ◽

2021 ◽

Vol 9 (1) ◽

pp. 79-92

Author(s):

L. N. Landina ◽

Keyword(s):

Pharmacological Study ◽

Vascular Wall ◽

Biologically Active ◽

Hypolipidemic Effect ◽

Individual Substance ◽

Dry Extract ◽

Original Product ◽

Active Substances ◽

Β Carotene

The objective is to standardize the content of β-carotene – one of the principal active substances with sufficient hypolipidemic activity in the resultant original product. Search for natural biologically active substances having a hypolipidemic effect is one of the topical questions in modern pharmacy. Most of the research is carried out by means of isolating individual substances from the plant object. We propose to study a native phytocomplex obtained from pumpkin pulp – a dry extract of pumpkin pulp juice. The following materials and methods were used: laboratory-obtained dry extract of pumpkin pulp juice and methods used to determine the qualitative and quantitative content of β-carotene in an individual substance. It was possible to adapt the methods applied to the analysis of an individual substance in relation to the resulting phytocomplex containing both lipophilic and hydrophilic fractions. The rapid method of quantitative determination of β-carotene described in the scientific literature was corrected. An effective alternative method for spectrophotometric determination of β-carotene in the phytocomplex has been developed. Phytocomplexes standardized for the main active substances can be used to obtain effective drugs and biologically active additives with minimal side effects. In addition, the expansion of the search for alternative natural objects containing complexes haaving a hypolipidemic effect is currently relevant. Based on the literature data, in the future it is planned to conduct a pharmacological study to establish the anti-inflammatory effect of the resulting complex on the state of the vascular wall.

Download Full-text

CRYOPRESERVATION OF OOCYTE-CUMULUS COMPLEXES OF COWS WITH DIFFERENT BIOACTIVE SUBSTANCES

Animal Breeding and Genetics ◽

10.31073/abg.51.34 ◽

2018 ◽

Vol 51 ◽

pp. 255-260

Author(s):

P. A. Trotskiy

Keyword(s):

Chromosomal Abnormalities ◽

Experimental Studies ◽

The Body ◽

Biologically Active Substances ◽

Biologically Active ◽

Bioactive Substances ◽

Fetal Serum ◽

Vitrification Solution ◽

Active Substances

Implementation of biotechnological process in livestock should be considered not only in terms of selection process intensification (obtain of embryos in vitro, their transplantation) and to a greater extent as development of effective methods of freezing and long storage of mammalian cells, including ova and embryos. Application of biotechnology in livestock breeding increases rate of genetic progress, preservation of gene pool of breeds via banks of sperm, embryos and cryobank of oocytes, obtaining and regulation of progeny of the desired sex, providing genetic evaluation of gametes and embryos, and it will enable to use genetic potential of animals after culling by age, replicate and create new genotypes with desired properties repeatedly. Solution to this problem is to improve medium and conditions of gametes and embryos freezing. Although the overall development of cryopreservation method is through simplification of the equilibration and vitrification solutions which would be able to ensure the full development frozen-thawed gametes. Addition of biologically active substances to the solution for cryopreservation contributes to protection of gametes during freezing and thawing, and determination of the consistent patterns of these substances will improve procedures of frozen-thawed oocyte cultivation outside the body. So it is necessary to deepen the fundamental research on the mechanisms of formation of a mature ovum of cows obtained from frozen-thawed oocytes to obtain embryos. The aim of the research is to conduct comparative analysis of different biologically active substances in equilibration and vitrification solutions at cryopreservation of oocyte-cumulus complexes of cows. Material and methods of the research. The objects of experimental studies were oocyte-cumulus complexes of black-and-white cows. The oocytes with homogeneous fine-grained ooplasm, undamaged pellucid zone, thick or partially loosened cumulus were used for freezing. The gametes of cows were treated by equilibration solution before freezing and then were transferred into vitrification solution. All the equilibration (10% glycerol + 20% propanediol) and vitrification (25% glycerol + 25% propanediol) solutions for cryopreservation of cows’ oocyte-cumulus complexes were prepared in Dulbecco phosphatebuffered saline with addition of 20% fetal serum of cows, 1х10-4 M unithiol, 1х10-6 M acetylcholine and without addition of bioactive substances. The research on adding some biologically active substances (fetal serum of cows – version A, unithiol – version B, acetylcholine – version C, without addition of biologically active substances – version D and not frozen cells of the control group (K)) in equilibration and vitrification solutions at freezing the oocyte-cumulus complexes of cows was carried out. It was found by the results of experimental studies that introduction of the above-mentioned components into the equilibration solution for freezing cows’ oocyte-cumulus complexes increased their cryoresistance, as evidenced by increasing indicator of maturation of frozen-thawed gametes outside the body to metaphase-2 of meiosis after 27-hour cultivation by 5,4-23,0% and decreasing indicator of number of oocytes with chromosomal abnormalities by 2,9-15,3%. The introduction of biologically active substances into the vitrification solution and subsequent cultivation during 27 hours after freezing and thawing cows’ oocyte-cumulus complexes showed that the indicator of maturation of frozen-thawed gametes outside the body to metaphase-2 of meiosis increased by 3,9-16,4% and the indicator of number of oocytes with chromosomal abnormalities decreased by 2,9-8,4%. Comparative analysis of the results of in vitro fertilization of cows’ frozen-thawed ova which were frozen using fetal serum of cows (version A) and without it (version - B) has shown a positive effect of adding it to equilibration and vitrification medium at freezing gametes of cows; it led to increase of obtaining bovine embryos in vitro by 11,5%. Thus, the analysis of experimental results showed different efficiency of using fetal serum of cows, unithiol, acetylcholine in the equilibration solution for freezing cows’ oocyte-cumulus complexes. The advantage of using these biologically active substances in the vitrification solution for cryopreservation of cow’s gametes by the indicator of maturation of frozen-thawed oocyte-cumulus complexes outside the body to metaphase-2 of meiosis wasn’t established. Conclusions. Introduction of fetal serum into cryopreservation solution increases cryoresistance of cow’s oocytes to cooling leading to increasing the indicator of the matured frozen-thawed gametes outside the body to metaphase-2 of meiosis by 23,0% and the obtained bovine embryos in vitro by 11,5%.

Download Full-text

Study of the Biologically Active Properties of Medicinal Plant Cotinus coggygria

Plants ◽

10.3390/plants10061224 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1224

Author(s):

Stanislav Sukhikh ◽

Svetlana Noskova ◽

Artem Pungin ◽

Svetlana Ivanova ◽

Liubov Skrypnik ◽

...

Keyword(s):

Mass Fraction ◽

Antioxidant Status ◽

Point Of View ◽

Biologically Active ◽

Bioactive Substances ◽

E Coli ◽

Acid Methyl ◽

Pentagalloyl Glucose ◽

Cotinus Coggygria ◽

Active Substances

The results of the studies have shown that to obtain an extract of a complex of biologically active substances of Cotinus coggygria, ethyl alcohol (mass fraction of alcohol 70%) with a hydromodule of 1:5 should be used, and the extraction should be carried out for 60 min at a temperature of 60 °C. The investigated plant extracts with the complex of bioactive substances from the Cotinus coggygria leaves and flowers are safe from the point of view of the content of heavy metals, pesticides, aflatoxin B1, radionuclides, as well as pathogenic and opportunistic microorganisms. It has been established that the Cotinus coggygria extract contains rutin, hyperoside, ferulic acid, quercetin, kaempferol, disulphuretin, sulphurein, sulphurein, gallic acid, methyl gallate, pentagalloyl glucose, 3,3′,4′,5,6,7-hexahydroxyflavonone, 3,3′,4′,5,5′,7-hexahydroxyflavonone, 3-O-α-L-rhamnofuranoside, 3,3′,4′,5,5′,7-hexahydroxyflavulium(1+), 7-O-β-D glucopyranoside, and 3,3′,4′,7-tetrahydroxyflavonone. The tested extracts have anticancer, antigenotoxic, and antimicrobial (against E. coli, S. aureus, P. vulgaris, C. albicans, L. mesenteroides) properties. The high antioxidant status of the tested extracts was established; the antioxidant activity of the samples was 145.09 mg AA/g (AA—ascorbic acid).

Download Full-text

Neutral Lipids of Oats Fruit (Avena Sativa L.)

Drug development & registration ◽

10.33380/2305-2066-2020-9-4-40-43 ◽

2020 ◽

Vol 9 (4) ◽

pp. 40-43

Author(s):

N. K. Yuldasheva ◽

S. D. Gusakova ◽

D. Kh. Nurullaeva ◽

N. T. Farmanova ◽

R. P. Zakirova ◽

...

Keyword(s):

Avena Sativa ◽

Neutral Lipids ◽

The Body ◽

Biologically Active ◽

Absorption Bands ◽

Vital Functions ◽

The Republic ◽

Living Organisms ◽

Main Components ◽

Active Substances

Introduction. Lipids are a widespread group of biologically active substances in nature, making up the bulk of the organic substances of all living organisms. They accumulate in plants in seeds, as well as in fruits and perform a number of vital functions: they are the main components of cell membranes and the energy reserve for the body.Aim. Study of neutral lipids of sown oats (Avena sativa L.).Materials and methods. The objects of the study were fruits (grains) of oats of the sown variety "Tashkent 1," harvested in the Republic of Uzbekistan. Results and discussions. Neutral lipids of oat grains have been found to contain 13 fatty acids with a predominance of the sum of oleic, linolenic and linoleic acids. The total degree of unsaturation was almost 78%. Absorption bands characteristic of these substances were observed in the IR spectrum of MEGC.Conclusion. According to the results of the NL analysis, oat grains consisted of triacylglycerides and free LCDs, which were accompanied by hydrocarbons, phytosterols, triterpenoids and tocopherols.

Download Full-text