scholarly journals Morphological characteristics of experimental ischemic wound healing after application of autoand hetero-fibroblasts and dermal equivalent

2018 ◽  
Vol 99 (1) ◽  
pp. 53-59
Author(s):  
E Yu Shapovalova ◽  
T A Boyko ◽  
Yu G Baranovskiy ◽  
I A Lugin
Keyword(s):  
Wound Healing ◽  
Morphological Characteristics ◽  
Dermal Equivalent

Цель. Изучить морфологическое строение, коллагенообразование и ангиогенез в биоптатах новообразованного эпидермиса и дермы на 19-е сутки их восстановления в экспериментальной ишемизированной ране после введения ауто- и гетерофибробластов, а также после трансплантации дермального эквивалента с гетерофибробластами. Методы. Исследование выполнено на 28 белых половозрелых мышах линии С57/В1 в возрасте до 1 года. Вокруг и в дно хирургической экспериментальной кожной раны в лопаточной области вводили 0,4 мл взвеси фибробластов и дермальный эквивалент в количестве 1,33 млн клеток. Биоптат заливали в парафин, окрашивали гематоксилином и эозином и по Вейгерту-Ван-Гизону. Результаты. Наиболее существенно на регенераторный гистогенез влияет введение аутофибробластов, когда фиксируется наибольшая толщина эпидермиса, наиболее активны ангиогенез и коллагенообразование. Вместе с тем, самым дифференцированным эпидермис становится после трансплантации в рану дермального эквивалента с гетерофибробластами. Вывод. Благоприятное воздействие дермального эквивалента с гетерофибробластами отличается от воздействия взвеси аутофибробластов всего на несколько процентов: толщина эпидермиса - на 4,29%, площадь коллагеновых волокон - на 2,66%, площадь кровеносных сосудов - на 4,04%, что делает такие различия недостоверными.

Comparative efficiency of physiotherapeutic and medicinal treatment of microelements during reparative skin regeneration

2021 ◽  
pp. 120-126
Author(s):  
М.М. Шарипова
Keyword(s):  
Wound Healing ◽  
Comparative Study ◽  
Morphological Characteristics ◽  
Skin Wound ◽  
Skin Regeneration ◽  
Wound Surface ◽  
Herbal Preparations ◽  
Comparative Efficiency ◽  
Medicinal Treatment ◽  
Positive Effect

Проведено сравнительное изучение влияния двух богатых микроэлементами растительных препаратов и облучения лампами полого катода (ЛПК), содержащими спектры магния и меди - ЛПК Mn&Cu в экспериментальной модели репаративной регенерации кожи. Наносимая на поверхность раны смесь препаратов Метосепт и Витанорм и их пероральное введение в дозах 40-60 мг/крысу на 30% ускоряет репаративную регенерацию кожи за счёт увеличения количества дендритных клеток и макрофагов в очаге воспаления. Ежедневное 2-минутное облучение ЛПК Mn&Cu приводило к положительному изменению динамики морфологических характеристик процесса регенерации кожных ран, ускорению дифференцировки эпителиальных клеток и также к 30%-му ускорению заживления ран. Сравнительное изучение эффективности излучения пяти фототерапевтических аппаратов показало, что уменьшение площади раневой поверхности быстрее всего происходило под влиянием магнитолазерного воздействия аппаратом «Орион+», 2-е место по этому показателю разделили аппараты «Минитаг» и ЛПК Zn&Mn, 3-е - ЛПК Zn и «Биоптрон». A comparative study was conducted of the effect of herbal preparations and irradiation with hollow cathode lamps (HCL) producing magnesium and copper spectra, i.e., the Mg & Cu HCL, in an experimental model of reparative skin regeneration. A mixture of two micronutrient-rich drugs, Metosept and Vitanorm, were applied to the surface of the wound. Their oral administration in doses of 40-60 mg/rat accelerated the reparative skin regeneration by 30% by increasing the number of dendritic cells and macrophages in the focus of inflammation. Daily 2-min irradiation of the Mg & Cu HCL had a positive effect on the dynamics of the morphological characteristics of the skin wound regeneration process. There occurred an acceleration of epithelial cell differentiation, and also a 30% acceleration of wound healing. A comparative study of the radiation efficiency of five phototherapy devices showed that the wound surface reduction most quickly occurred during treatment with the magnetic laser “Orion+” apparatus. The 2nd most effective treatment was Minitag and HCL Zn & Mg, and the 3rd most effecivet was HCL Zn and Bioptron.

Electrospinning of chitosan-based nanofibers: from design to prospective applications

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Alexandru Anisiei ◽  
Florin Oancea ◽  
Luminita Marin
Keyword(s):  
Drug Delivery ◽  
Wound Healing ◽  
Renewable Resources ◽  
Food Packaging ◽  
Morphological Characteristics ◽  
Active Surface ◽  
Advantages And Disadvantages ◽  
Electrospinning Method ◽  
Attractive Candidate ◽  
Controlled Morphology

Abstract Chitosan is a biopolymer originating from renewable resources, with great properties which make it an attractive candidate for plenty of applications of contemporary interest. By manufacturing chitosan into nanofibers using the electrospinning method, its potential is amplified due to the enhancement of the active surface and the low preparation cost. Many attempts were made with the aim of preparing chitosan-based nanofibers with controlled morphology targeting their use for tissue engineering, wound healing, food packaging, drug delivery, air and water purification filters. This was a challenging task, which resulted in a high amount of data, sometimes with apparent contradictory results. In this light, the goal of the paper is to present the main routes reported in the literature for chitosan electrospinning, stressing the advantages and disadvantages of each of them. Special emphasis is placed on the influence of various electrospinning parameters on the morphological characteristics of the fibers and their suitability for distinct applications.

Silver sulfadiazine loaded breathable hydrogel sponge for wound healing

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Anamika Pandey ◽  
Munira Momin ◽  
Anita Chando
Keyword(s):  
Wound Healing ◽  
Drug Release ◽  
Ex Vivo ◽  
Release Kinetics ◽  
Antimicrobial Property ◽  
Morphological Characteristics ◽  
Silver Sulfadiazine ◽  
Scanning Calorimetry ◽  
Casting Technique

AbstractObjectivesPatients with serious injury need special care and treatment to control the infection, as wound sepsis is one of the major causes of death. Silver sulfadiazine (SSD) is widely used as an antimicrobial agent which promotes healing and re-epithelialization. However, due to certain drawbacks such as inflammation and cytotoxicity, the need for novel drug delivery modality emerges. The objective of this study was to develop natural polymeric (chitosan and gelatin) hydrogel sponges containing SSD and evaluate its efficacy in wound healing using animal models.MethodsSSD containing hydrogel sponges were prepared by solvent casting technique. Scanning electron microscopy (SEM) and Differential scanning calorimetry (DSC) were used to evaluate morphological characteristics of the hydrogel sponges. Anti-thrombogenic property, drug release studies, drug release kinetics, antimicrobial property, and wound healing effect were also studied in detail.ResultsThe optimized batch of hydrogel sponges (CG4) consists of 1% SSD wt., 10% wt. Gelatin, 1% wt. Chitosan and honey 7.5% wt. as plasticizer. At the 12th hour, in vitro and ex vivo drug release was found to be 76.994±0.67% and 24.22±0.57% respectively. CG4 batch had enhanced in vitro antimicrobial activity as compared to conventional marketed cream. The developed SSD hydrogel sponges showed a faster rate of wound healing as compared to a marketed cream. Animals treated with CG4 formulation showed complete angiogenesis and re-epithelialization by 8th day, whereas 12 days were required for complete wound healing with marketed cream.ConclusionsThe prepared hydrogel sponges can serve as a potential alternative for wound healing dressing as compared to the marketed product.

scholarly journals Clinical and morphological characteristics of wound healing in diabetic foot syndrome

10.14341/7704 ◽  
2017 ◽  
Vol 20 (2) ◽  
pp. 135-141 ◽  
Author(s):  
Elena Yur'evna Komelyagina ◽  
Eugenia Alexandrovna Kogan ◽  
Mikhail Borisovich Antsiferov
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Diabetic Foot ◽  
Morphological Characteristics ◽  
Glycated Haemoglobin ◽  
Late Complications ◽  
Ki 67 ◽  
Fibrotic Tissue ◽  
Significant Difference ◽  
Time Of Admission

Aim. To assess the clinical and morphological characteristics of neuropathic diabetic foot ulcers (DFUn) Materials and Methods.Forty-three specimens of DFUn were analysed. Depending on the outcome, samples were divided into 2 groups: group 1healed ulcers (30 samples), group 2unhealed ulcers (13 samples). The following characteristics were analysed: age, sex, glycated haemoglobin (HbA1c), ulcer duration, time of admission to the specialised clinic, severity of neuropathy, presence of late complications, plantar localisation, percentage of necrotic tissue, granulation and fibrotic tissue and, Ki-67expression. Results. Patients were similar in age, HbA1c, severity of late complications and plantar localisation. There was a significant difference in the time of admission to the specialised clinic [50 vs 132 days, p = 0.03], percentage of granulation [61 25 vs 32 21 %, р = 0.001] and fibrotic tissue [24 24 vs 49 22 %, р = 0.002], the granulation/fibrosis coefficient [7.5 8.1 vs 1.9 4.6 (%), р = 0,02] and expression of Ki-67 [15 8 vs 21 (%), р = 0.001] between groups. A multidimensional model revealed granulation tissue as the main parameter influencing healing. The probability of healing was 1.0 if the percentage of granulation tissue was 50%. If the percentage of granulation tissue was 50%, the prognosis of healing was determined by the expression of Ki-67. When Ki-67 levels were 7%, the probability of healing was 0.75. For Ki-67 levels 7%, the probability of healing was 0.17 and the probability of not healing was 0.83. Conclusion. The time at which a patient was admitted to the specialised clinic and the percentage of granulation tissue were key factors affecting the prognosis of wound healing in DFUn.

scholarly journals Quantity Differences of Secondary Metabolites (Saponins, Tannins, and Flavonoids) from Binahong Plant Extract (Anredera cordifolia (Ten.) Steenis) treated and untreated with Colchicines that play a Role in Wound Healing

2017 ◽  
Vol 8 (4) ◽  
pp. 296-299
Author(s):  
Olivia A Hanafiah ◽  
Diana S Hanafiah ◽  
Eva S Bayu ◽  
Syafrudin Ilyas ◽  
Marline Nainggolan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Secondary Metabolites ◽  
Plant Extract ◽  
Leaf Extract ◽  
Healing Process ◽  
Morphological Characteristics ◽  
Plant Morphology ◽  
Phytochemical Analysis ◽  
Collagen Formation ◽  
Speed Up

ABSTRACT Aim The aim of this study is to compare whether there are differences in the value content of secondary metabolites (saponins, tannins, and flavonoids) between the group treated with Binahong leaves (administration of colchicines) and the group treated with Binahong leaves without the application of colchicines. Materials and methods The design of this study is an experimental research laboratory study. This research was done in four different places in which colchicines were applied to improve the quality of Binahong, conducted in the USU Agricultural Faculty's Greenhouse, whereas no application of colchicines was done in the Desa Simpang Pergendangan Kelurahan Tiga Binanga Kabupaten Karo harvesting Binahong plant. The pharmacy faculty of USU was chosen as a place to obtain the extract and the Laboratory of the Research Institute for Spices and Medicinal Plants (Balitro) was where the phytochemical analysis was conducted. Results The results showed that there were differences in the descriptive values of secondary metabolites in the Binahong leaf extract, such as saponins, tannins, and flavonoids, wherein the group with colchicines was higher than groups without colchicines, but there was no statistical differences between the groups treated with colchicines and those not treated with colchicines (value p > 0.05). Conclusion Administration of colchicines can improve the content of secondary metabolites in Binahong leaf extract and the plant morphology without affecting the content of the plant extract. Clinical significance Secondary metabolites in herbal plants, such as saponins, flavonoids, and tannins are able to speed up the healing process. One of the plants that have all three of these active compounds is Binahong (Anredera cordifolia). Binahong leaf extract can stimulate the process of fibroblasts and collagen formation that will accelerate the process of wound healing. Colchicine as a reagent for the mutation is able to affect the number of leaves and morphological characteristics of the Binahong plant. How to cite this article Hanafiah OA, Hanafiah DS, Bayu ES, Abidin T, Ilyas S, Nainggolan M, Syamsudin E. Quantity Differences of Secondary Metabolites (Saponins, Tannins, and Flavonoids) from Binahong Plant Extract (Anredera Cordifolia (Ten.) Steenis) treated and untreated with Colchicines that play a Role in Wound Healing. World J Dent 2017;8(4):296-299.

Cell Wall Ultrastructure in Three Strains of a Cariogenic Streptococcus

1971 ◽  
Vol 29 ◽  
pp. 338-339
Author(s):  
M. J. Kramer ◽  
Alan L. Coykendall
Keyword(s):  
Cell Wall ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Genetic Heterogeneity ◽  
Morphological Characteristics ◽  
Electron Microscopic ◽  
Dna Reassociation ◽  
Wall Ultrastructure

During the almost 50 years since Streptococcus mutans was first suggested as a factor in the etiology of dental caries, a multitude of studies have confirmed the cariogenic potential of this organism. Streptococci have been isolated from human and animal caries on numerous occasions and, with few exceptions, they are not typable by the Lancefield technique but are relatively homogeneous in their biochemical reactions. An analysis of the guanine-cytosine (G-C) composition of the DNA from strains K-1-R, NCTC 10449, and FA-1 by one of us (ALC) revealed significant differences and DNA-DNA reassociation experiments indicated that genetic heterogeneity existed among the three strains. The present electron microscopic study had as its objective the elucidation of any distinguishing morphological characteristics which might further characterize the respective strains.

Aberrant Type C Virus Production in a Cell Line Derived from Balb/3T3

1972 ◽  
Vol 30 ◽  
pp. 286-287
Author(s):  
N. Savage ◽  
A. Hackett
Keyword(s):  
Electron Microscopy ◽  
Cell Line ◽  
Leukemia Virus ◽  
Morphological Characteristics ◽  
Virus Production ◽  
Oncogenic Viruses ◽  
Endogenous Virus ◽  
Virus Particles ◽  
Moloney Leukemia Virus ◽  
A Cell

A cell line, UC1-B, which was derived from Balb/3T3 cells, maintains the same morphological characteristics of the non-transformed parental culture, and shows no evidence of spontaneous virus production. Survey by electron microscopy shows that the cell line consists of spindle-shaped cells with no unusual features and no endogenous virus particles.UC1-B cells respond to Moloney leukemia virus (MLV) infection by a change in morphology and growth pattern which is typical of cells transformed by sarcoma virus. Electron microscopy shows that the cells are now variable in shape (rounded, rhomboid, and spindle), and each cell type has some microvilli. Virtually all (90%) of the cells show virus particles developing at the cell surface and within the cytoplasm. Maturing viruses, typical of the oncogenic viruses, are found along with atypical tubular forms in the same cell.

DNA nick end labeling: a reliable marker for apoptosis?

1995 ◽  
Vol 53 ◽  
pp. 962-963
Author(s):  
Anne F. Bushnell ◽  
Sarah Webster ◽  
Lynn S. Perlmutter
Keyword(s):  
Cell Death ◽  
Cultured Cells ◽  
Apoptotic Cell ◽  
Morphological Characteristics ◽  
Detection Methods ◽  
Tissue Preparation ◽  
Preparation Methods ◽  
Dna Laddering ◽  
Disease States ◽  
Reliable Marker

Apoptosis, or programmed cell death, is an important mechanism in development and in diverse disease states. The morphological characteristics of apoptosis were first identified using the electron microscope. Since then, DNA laddering on agarose gels was found to correlate well with apoptotic cell death in cultured cells of dissimilar origins. Recently numerous DNA nick end labeling methods have been developed in an attempt to visualize, at the light microscopic level, the apoptotic cells responsible for DNA laddering.The present studies were designed to compare various tissue processing techniques and staining methods to assess the occurrence of apoptosis in post mortem tissue from Alzheimer's diseased (AD) and control human brains by DNA nick end labeling methods. Three tissue preparation methods and two commercial DNA nick end labeling kits were evaluated: the Apoptag kit from Oncor and the Biotin-21 dUTP 3' end labeling kit from Clontech. The detection methods of the two kits differed in that the Oncor kit used digoxigenin dUTP and anti-digoxigenin-peroxidase and the Clontech used biotinylated dUTP and avidinperoxidase. Both used 3-3' diaminobenzidine (DAB) for final color development.

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