scholarly journals Quantity Differences of Secondary Metabolites (Saponins, Tannins, and Flavonoids) from Binahong Plant Extract (Anredera cordifolia (Ten.) Steenis) treated and untreated with Colchicines that play a Role in Wound Healing

2017 ◽  
Vol 8 (4) ◽  
pp. 296-299
Author(s):  
Olivia A Hanafiah ◽  
Diana S Hanafiah ◽  
Eva S Bayu ◽  
Syafrudin Ilyas ◽  
Marline Nainggolan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Secondary Metabolites ◽  
Plant Extract ◽  
Leaf Extract ◽  
Healing Process ◽  
Morphological Characteristics ◽  
Plant Morphology ◽  
Phytochemical Analysis ◽  
Collagen Formation ◽  
Speed Up

ABSTRACT Aim The aim of this study is to compare whether there are differences in the value content of secondary metabolites (saponins, tannins, and flavonoids) between the group treated with Binahong leaves (administration of colchicines) and the group treated with Binahong leaves without the application of colchicines. Materials and methods The design of this study is an experimental research laboratory study. This research was done in four different places in which colchicines were applied to improve the quality of Binahong, conducted in the USU Agricultural Faculty's Greenhouse, whereas no application of colchicines was done in the Desa Simpang Pergendangan Kelurahan Tiga Binanga Kabupaten Karo harvesting Binahong plant. The pharmacy faculty of USU was chosen as a place to obtain the extract and the Laboratory of the Research Institute for Spices and Medicinal Plants (Balitro) was where the phytochemical analysis was conducted. Results The results showed that there were differences in the descriptive values of secondary metabolites in the Binahong leaf extract, such as saponins, tannins, and flavonoids, wherein the group with colchicines was higher than groups without colchicines, but there was no statistical differences between the groups treated with colchicines and those not treated with colchicines (value p > 0.05). Conclusion Administration of colchicines can improve the content of secondary metabolites in Binahong leaf extract and the plant morphology without affecting the content of the plant extract. Clinical significance Secondary metabolites in herbal plants, such as saponins, flavonoids, and tannins are able to speed up the healing process. One of the plants that have all three of these active compounds is Binahong (Anredera cordifolia). Binahong leaf extract can stimulate the process of fibroblasts and collagen formation that will accelerate the process of wound healing. Colchicine as a reagent for the mutation is able to affect the number of leaves and morphological characteristics of the Binahong plant. How to cite this article Hanafiah OA, Hanafiah DS, Bayu ES, Abidin T, Ilyas S, Nainggolan M, Syamsudin E. Quantity Differences of Secondary Metabolites (Saponins, Tannins, and Flavonoids) from Binahong Plant Extract (Anredera Cordifolia (Ten.) Steenis) treated and untreated with Colchicines that play a Role in Wound Healing. World J Dent 2017;8(4):296-299.

scholarly journals Syzygium Samarangense Leaves Ointment Enhances Wound Healing Process Of Skin Burn Based On Collagen

2017 ◽  
Vol 3 (3) ◽  
pp. 30
Author(s):  
Ain Yuanita Insani ◽  
Mega Citra Prameswari ◽  
Novail Alif Muharrom ◽  
Toyibatul Hidayati ◽  
Arista Prima Nugrahani ◽  
...  
Keyword(s):  
Wound Healing ◽  
Leaf Extract ◽  
Healing Process ◽  
Multiple Organ ◽  
World Health ◽  
Wound Healing Process ◽  
Skin Damage ◽  
Collagen Formation ◽  
Organ Failures

Background: The World Health Organization (WHO) estimates the number of death in 2014 caused by burns is 265,000. Burns can caused skin damage as well as other complication problems such as dehydration, infection, and other multiple organ failures. Syzygium samarangense leaves contain flavonoids and saponins that can increase the activation of macrophages and TGF-B which is important to accelerate the process of collagen formation and wound healing process. Objective: Knowing the effect of Syzygium samarangense leaf extract on the healing process of burn based on collagen. Method: This in vivo study use true experimental design. We made burns by placing a coin that already heated in oven at 70ºC for 10 seconds. Rattus Wistar as experimental animals dividedinto 6 groups (n = 4) with details of Group A (normal), B (positive), C (negative). Groups D, E, and F were the groups that given ointment extract topically in doses of 15%, 30%, and 45% each day’s. Termination is done on day 7. Test statistics by using Anova and Kruskal Wallis. Results and discussion: From this research, Syzygium samarangense leaf extract can reduce the wound area (p <0,05) and increase the amount of collagen (p <0,05). Conclusion: Syzygium samarangense leaf extract ointment can accelerate the healing process of burns. Keywords : Burns, Syzygium samarangense Leaves ointment, Saponin, Flavonoid, Collagen

scholarly journals Wound-Healing potential of Sebastiania hispida (Mart.) Pax (Euphorbiaceae) ointment compared to low power laser in rats

2016 ◽  
Vol 77 (3) ◽  
pp. 480-489 ◽  
Author(s):  
E. S. Rizzi ◽  
D. M. Dourado ◽  
R. Matias ◽  
J. A. I. Muller ◽  
J. F. Guilhermino ◽  
...  
Keyword(s):  
Wound Healing ◽  
Plant Extract ◽  
Healing Process ◽  
Controlled Study ◽  
Epithelial Tissue ◽  
Phytochemical Analysis ◽  
Fiber Types ◽  
Impaired Wound Healing ◽  
Inflammatory Phase ◽  
Muscle Fascia

Abstract Impaired wound healing represents a serious complication in some pathologies and the use of plant extracts has proved to improve tissue repair. The objectives of this study were to evaluate the healing potential of the ointment of Sebastiana hispida compared with Aluminum-Gallium Indium-Phosphide Laser (InGaAlP) in surgically induced wounds in rats and to perform the phytochemical analysis. The phytochemical analysis was performed in the classic way and also by HPLC. A controlled study was developed with 80 rats (200-250 g) in which a linear excision was performed in the dorsal region after shaving, measuring 2 cm × 1 cm (epidermis and dermis) exposing the muscle fascia. The rats were randomly divided into four groups of twenty animals each. The experimental groups (n = 5) were G1 (Saline); G2 (crude methanol plant extract 2% + Carbopol Gel 98%); G3 (crude methanol plant extract 2% + lanolin/vaseline) and G4 (laser). The incision healing processes were monitored during 3, 7, 14 and until 21 days after excision. The histologic parameters evaluated were Collagen fiber types, microscopic examination and neovascularization. There was a significant increase in the deposition of collagen fibers, as evidenced by a better organized epithelial tissue, keratinized and showing greater proliferation of new blood vessels in the inflammatory phase in the group treated with both the extract and laser. The results were correlated to the phenolic derivatives found after qualitative and quantitative analysis. These compounds were considered responsible for the healing process. The topical treatment with S. hispida leaves, in the two different formulations, was more effective than the application of the laser (Ingan ALP) 660 nm in the model used.

scholarly journals Pharmacognostical, Phytochemical, Antioxidant and Antimicrobial Activity of Costus woodsonii

2019 ◽  
pp. 53-59
Author(s):  
Mukesh S Sikarwar ◽  
Chew Khai Szeek ◽  
Neeraj Paliwal
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Plant Extract ◽  
Total Phenolic Content ◽  
Leaf Extract ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Concentration Test ◽  
Antioxidant And Antimicrobial Activity

Background: Herbal medicine mostly contains wide range of chemical compounds responsible for medicinal therapeutic use. Costus woodsonii is commonly called as Red Button Ginger and synonyms of the botanical name are Costus spiralis, Alpinia spiralis and Costus pisonis. In Malay, it is known as Setawar Halia Merah. In Chinese, it is known as Hong Bi Qiao Jiang. Objective: This research was conducted to study the pharmacognostical, phytochemical, antioxidant and antimicrobial activity of C. woodsonii leaf extracts. Method: Macroscopy, microscopy, phytochemical analysis, thin layer chromatography, antioxidant activity and antimicrobial activity of C. woodsonii leaf were carried out. Total flavonoids were estimated in the leaf extract. The total phenolic content of C. woodsonii leaf was determined using Folin-Ciocalteu reagent. The antioxidant activity of leaf extract of C. woodsonii was determined by performing DPPH radical scavenging. The microbial activity was determined by Well diffusion test, MIC (Minimum Inhibitory Concentration) test and MBC (Minimum Bactericidal Concentration) test. Result and Discussion: C. woodsonii belongs to costaceae with elliptical green leaves. Till now are no extensive studies on C. woodsonii. Preliminary phytochemical analysis revealed the presence of flavonoid, steroid, fat, phenol, tannin and mucilage in leaf extract. Physicochemical studies further revealed the ash value of leaf as 8.7%. Among the three extractions, alcohol extractive values showed the highest as 13%. Loss on drying at 105 degree Celsius in leaf was found to be 12.67%. The plant extract showed total phenolic content of 7.941 mg GAE/g at concentration of 5µg/ml. As for flavonoids content, plant extract showed 21.7 mg RE/g at concentration of 200µg/ml and 43.4 mg RE/g at concentration of 400µg/ml. For antioxidant activity, the plant extract showed weak antioxidant activity in DPPH scavenging activity assay. For antimicrobial test, the leaf extract of C. woodsonii showed weak antimicrobial activity. Conclusion: From this study, it can conclude that C. woodsonii leaf extract possess weak antioxidant activity and weak antimicrobial activity which need to be further validated by using more antioxidant assays and antimicrobial tests.

scholarly journals PHYTOCHEMICAL INVESTIGATION, GC-MS PROFILE AND ANTIMICROBIAL ACTIVITY OF A MEDICINAL PLANT RUTA GRAVEOLENS L. FROM ETHIOPIA

2017 ◽  
Vol 9 (6) ◽  
pp. 29 ◽  
Author(s):  
Henok Gulilat Azalework ◽  
Sahabjada . ◽  
Asif Jafri ◽  
Md Arshad ◽  
Tabarak Malik
Keyword(s):  
Secondary Metabolites ◽  
Leaf Extract ◽  
Micrococcus Luteus ◽  
Biological Activities ◽  
Antimicrobial Effect ◽  
Phytochemical Analysis ◽  
Ruta Graveolens ◽  
Zone Of Inhibition ◽  
Ms Analysis

Objective: This study was designed to screen the phytochemicals present in various solvents extracts of Ruta graveolens (Rue) and furthermore to investigate their antimicrobial activity.Methods: The leaves, stems and seeds of Rue were extracted using four different solvents viz. ethanolic, methanolic, chloroform, and aqueous of varying polarity. The phytochemical screening was carried out qualitatively and Gas Chromatography-Mass Spectroscopy (GC-MS) analysis was performed to identify major phytoconstituents present in the methanolic leaf extract. The antimicrobial effect of extracts was evaluated against six microbial strains namely Bacillus subtillis, Escherichia coli, Proteus vulgaris, Candida albicans, Candida tropicalis and Micrococcus luteus with disc diffusion method.Results: Phytochemical analysis revealed the presence of various secondary metabolites such as flavonoids, alkaloids, terpenoids, saponins and carotenoid. The methanolic leaf extract showed the presence of both tannin and phenolic contents in the higher amount, whereas aqueous extract displayed in the least amount. GC-MS analysis of methanolic leaf extract revealed the presence of approximately 26 phytochemical constituents. The antimicrobial assay revealed that B. subtilis showed a high zone of inhibition (20 mm) at 200 mg/ml of methanolic extract. However, E. coli and C. tropicalis did not show any zone of inhibition against each solvent extract.Conclusion: In conclusion, secondary metabolites present in the extracts have biological activities which warrant further to evaluate in vivo pharmacological studies.

Phytochemical Analysis and Wound-Healing Activity of Noni (Morinda Citrifolia) Leaf Extract

2020 ◽  
Vol 26 (4) ◽  
pp. 379-393
Author(s):  
Hai Trieu Ly ◽  
Minh Trang Pham Nguyen ◽  
Thi Kim Oanh Nguyen ◽  
Thi Phuong Quynh Bui ◽  
Xu Ke ◽  
...  
Keyword(s):  
Wound Healing ◽  
Leaf Extract ◽  
Morinda Citrifolia ◽  
Phytochemical Analysis ◽  
Wound Healing Activity

scholarly journals Antibacterial Test of Binahong Leaf Extract Ointment (Anredera cordifolia) Against Staphylococcus aureus Bacteria from Diabetic Wounds

2020 ◽  
Vol 5 (01) ◽  
pp. 1-10
Author(s):  
Risa Umami ◽  
Riwayati Malika
Keyword(s):  
Staphylococcus Aureus ◽  
Wound Healing ◽  
Leaf Extract ◽  
Wound Closure ◽  
Healing Process ◽  
The Body ◽  
Wound Healing Process ◽  
Antibacterial Test ◽  
Glucose Levels ◽  
Diabetic Wounds

Diabetes mellitus (DM) is characterized by an increase in glucose levels in the blood due to disorders of glucose metabolism in the body. The pancreas organ of people with DM has a weakness in producing the hormone insulin. As a result, the distribution of blood glucose to other organs of the body is inhibited so that glucose levels in the blood increase which causes DM sufferers to experience longer wound healing than normal humans. Binahong leaves contain alkaloids, saponins and flavonoids which have antibacterial activity which can accelerate the wound healing process. The purpose of this study was to determine the antibacterial effect of binahong leaf extract ointment (Anredera cordifolia) against Staphylococcus aureus bacteria from diabetic wounds. This research includes antibacterial test for binahong leaf extract ointment (Anredera cordifolia) with variations in the concentration of 10%, 20%, and 30% of the concentration of binahong leaf extract of 25% which resulted in a wound coverage percentage of up to 100%, namely at 30% ointment concentration. The conclusion of this study is that there was no wound closure for negative control and positive control in the form of oxytetracycline, the percentage of wound closure was 85%.

scholarly journals An assessment of the wound healing potential of a herbal gel containing an Azadirachta indica leaf extract

2021 ◽  
Vol 66 (No. 3) ◽  
pp. 99-109
Author(s):  
M Munir ◽  
SNH Shah ◽  
U Almas ◽  
FA Khan ◽  
A Zaidi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Leaf Extract ◽  
Healing Process ◽  
Ethanolic Extract ◽  
Physicochemical Characteristics ◽  
Wound Healing Process ◽  
E Coli ◽  
Carbopol 940 ◽  
Gel Formulations

The objective of this study was to produce a Carbopol 940 based gel formula containing an Azadirachta indica leaf extract and evaluate its wound healing potential. The ethanolic extract was derived from the dried leaves of Azadirachta indica and was subjected to a phytochemical evaluation. Three gel formulations of Carbopol 940 containing an Azadirachta indica extract in three different concentrations, i.e., 1, 2, and 3% w/w were prepared. These gels were evaluated for their physical appearance, stability, antimicrobial activity, extrudability, skin irritability, pH, spreadability, and viscosity. The prepared formulas were stable, greenish and homogeneous. None of them showed irritation to the skin. The spreadability (g.cm/sec), viscosity (cps), and pH of all three formulations was 34.68, 53 270–65 400, and 6–7, respectively. Gel-III exhibited the highest antimicrobial potential against E. coli and P. aeruginosa with a zone of inhibition of 16.2 ± 0.6 mm and 15.6 ± 0.6 mm, respectively. It was revealed from the wound healing studies that the epithelialisation time for the Albino rabbits treated with Gel-III was 23 days. The Albino rabbits treated with Gel-I, Gel-II, a standard gel, and those with the untreated one (control), epithelialised in 27, 25, 26, and 34 days, respectively. A formulation containing 3% w/w extract showed better antimicrobial activity, physicochemical characteristics, and pharmacological parameters than the other formulations. It can be concluded that the wound healing process was faster with the gel formulation containing 3% w/w of the Azadirachta indica extract, proposing that this formulation is a promising candidate for wound healing.

scholarly journals COMPARISON OF THE EFFECTIVENESS OF GIVING BINAHONG LEAF (Anredera cordifolia (Ten) Steenis) AND PAPAYA LEAF (Carica papaya) ON SKIN WOUND HEALING IN WHITE RAT (Rattus novergicus)

2021 ◽  
Vol 3 (2) ◽  
pp. 78-85
Author(s):  
Givenchy Winarjo ◽  
◽  
Fransiscus Arifin ◽  
Dave Oenarta ◽  
◽  
...  
Keyword(s):  
Wound Healing ◽  
Leaf Extract ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
P Value ◽  
World Population ◽  
White Rats ◽  
Significant Difference ◽  
The World

Introduction: The incidence of acute and chronic injuries increases every year. Most injuries in the world population, as many as 48%, are caused by trauma or surgery. the highest incidence of wounds in the world based on the etiology was from surgery, which was 110.30 million cases. One of the handlings of wounds on the skin can be done by giving herbal medicines, which can be easily accessed by the public, such as binahong leaves and papaya leaves. Purpose: To analyze the effectiveness of giving 40% binahong leaf extract cream and 40% papaya leaf extract cream on the wound distance in the white rat wound healing process. Method: this study is an experimental study with post-test only control group design approach. The sampling technique used in this study was a random sampling method where the number of samples was calculated using the Federer formula, with a total sample of 28 white rats. The distance of the wound was measured using a micrometer. Results: Twenty-eight wound distance on rats were measured as subjects of the study and then treated with binahong leaf extract cream and papaya leaf extract cream, then the wound distance of each treatment was measured using a micrometer. According to the results, 40% binahong leaf extract cream and 40% papaya leaf extract cream showed better effectiveness in reducing the wound distance of white rat incision than the negative control group (cream base) and positive control group (gentamicin ointment). According to the Mann Whitney test comparing the P1 group (40% binahong leaf extract cream) and P2 (40% papaya leaf extract cream), p-value >0.05, which is 0.543, which means that P1 has no significant mean difference with P2. Conclusion: There was no significant difference in effectiveness between the treatment of 40% binahong leaf extract cream and 40% papaya leaf extract cream.

scholarly journals Using tilapia skin (Oreochromis niloticus) as an occlusive biological curative in equine wounds:

2020 ◽  
Vol 56 (4) ◽  
pp. e154079 ◽  
Author(s):  
Sofia Cicolo da Silva ◽  
Vívian Fratti Penna Ríspoli ◽  
Cesar Graner ◽  
Lilian Rose Marques de Sá ◽  
Carla Bargi Belli ◽  
...  
Keyword(s):  
Wound Healing ◽  
Chronic Wound ◽  
Healing Process ◽  
Biological Factor ◽  
Histopathological Analysis ◽  
Environment Pollution ◽  
Wound Area ◽  
Area Reduction ◽  
Speed Up ◽  
Complete Wound Healing

Tilapia skin is being already use in humans and wild animals present burning wounds and showed a great result. The objective is to evaluate if tilapia skin used as an occlusive curative improves equine wound healing in two horses present chronic wound. Both animals are males, adults, both of breed Mangalarga Marchador South America. Every seven days wound we measured, photographed, biopsied for histopathological analysis, cleaned and tilapia curative was changed. Image J software was used to measure wound area. Tilapia skin as an occlusive biological factor seemed to improve healing process, wounds present an area reduction and clinical improvement during 35 days treatment, even though is still waiting for complete wound healing. In equine tilapia skin curative seemed to speed up healing process and allowed reduced curative change from every two days to once a week. This implies in decrease animal´s stress, less pain and treatment cost reduction since we used less bandage amount. Beside that tilapia skin industrial waste. Furthermore, it allowed avoid using antibiotics, which reduces environment pollution and there´s no antibiotic resistance issues.

scholarly journals Triticum aestivum in open skin wounds: cytotoxicity and collagen histopathology

2018 ◽  
Vol 39 (4) ◽  
pp. 1547
Author(s):  
Mariana Teixeira Tillmann ◽  
Cláudia Beatriz De Mello Mendes ◽  
Geferson Fischer ◽  
Antonio Sergio Varela Júnior ◽  
Cristina Gevehr Fernandes ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Control Cell ◽  
Negative Control ◽  
In Vitro Test ◽  
Skin Wounds ◽  
In Vivo Test ◽  
Collagen Formation

Phytoterapic compounds have been used in wound healing for many centuries. Nowadays, scientific evidences of phytotherapeutics is a requirement of the legislation. The scientific literature notes the need for healing topics yielding scars that are both aesthetically appealing and resistant. We aimed to evaluate the cytotoxicity of several doses of T. aestivum extract (2 mg mL-1, 4 mg mL-1, 6 mg mL-1, 8 mg mL-1 and 10 mg mL-1) in a fibroblast cell line and the healing process in an in vivo experimental model (New Zealand rabbits). For this, MTT test in 3T6 cells was performed in duplicates using MEM (0 mg ml-1) as negative control. Cell viability was calculated as: absorbance average in treatments/absorbance average in controls x 100. In vivo test was performed in 78 skin wounds in rabbits that were treated with 2 mg ml-1and 10 mg ml-1 of T. aestivum and non-ionic cream for 21 days. After this period, it was evaluated the histology using picrosorius and Gomori’s trichrome staining. Statistical analysis was evaluated using T test (Graphpad) for cytotoxicity assay, Fischer test for the gomori trichrome test (Grahpad) and Kruskal-Wallis (Statistic 9.0) for picrosirius test. The in vitro test resulted in cytotoxicity observed at 2mg mL-1 whereas cells were viable at higher doses. On the other hand, it was observed that collagen formation of wounds was more uniform with this dose than with 10mg mL-1 extract in the in vivo study. Thus, we conclude that the 2mg mL-1 T. aestivum aqueous extract dose was more efficient in the in vivo wound healing study, despite its cytotoxic effects in vitro.

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