scholarly journals Protein-Energy Synchronization Index of Various Energy Source of Feed Concentrate for Ruminants

2021 ◽  
Vol 21 (2) ◽  
pp. 172-177
Author(s):  
Afduha Nurus Syamsi ◽  
Hermawan Setyo Widodo ◽  
Harwanto Harwanto
Keyword(s):  
Energy Source ◽  
Rice Bran ◽  
Rumen Fluid ◽  
Proximate Analysis ◽  
In Vitro Digestibility ◽  
Synchronization Index ◽  
High Category ◽  
Protein Energy ◽  
Energy Synchronization

ABSTRACT. The study aimed to determine the protein-energy synchronization (PES) index of the various energy source of feed concentrate in vitro, as a database for the preparation of ruminant rations based on the PES index. The research was conducted from May to August 2020. The research was carried out experimentally through three stages: proximate analysis, in vitro digestibility test, and index calculation. The materials used were rumen fluid of three Jawa Randu Goats, taken shortly after being slaughtered at Sokaraja Goat Slaughterhouse, and seven types of energy sources of feed concentrate. Each feedstuff was duplicated for 3 replications, then the digestibility data for organic matter and protein of each feedstuff were collected at the 2nd, 4th, 6th, 8th, 12th, 24th, and 48th hour by in vitro fermentation. The data collection results were then regressed and the results were included in the PES index equation. The variable measured was the PES synchronization index. The results showed that the PES index of rice bran was 0.54, rice polish 0.50, pollard 0.57, corn 0.87, dried cassava 0.94, dried cassava dreg 0.90, and bread flour 0.94. The study concluded that the energy source of feed concentrate are potential as the medium to the high category of PES index values in the preparation of ruminant rations, where corn, dried cassava, dried cassava dreg, and bread flour have a PES index in the high category, while rice bran, rice polish, and pollard in the medium category.  (Indeks sinkronisasi protein-energi berbagai bahan pakan konsentrat sumber energi bagi ruminansia) ABSTRAK. Tujuan penelitian ini adalah menginventarisasi indeks sinkronisasi protein-energi (SPE) berbagai bahan pakan konsentrat sumber energy, sebagai basis data penyusunan ransum ruminansia berbasis indeks SPE secara in vitro. Penelitian dilaksanakan mulai bulan Mei hingga Agustus 2020. Penelitian dilaksanakan secara eksperimental melalui tiga tahap yaitu analisis proksimat, kecernaan in vitro, dan perhitungan indeks. Materi yang digunakan adalah cairan rumen 3 Kambing Jawa Randu yang diambil sesaat setelah disembelih di Rumah Potong Hewan Sokaraja, serta 7 jenis bahan pakan konsentrat sumber energi. Masing-masing bahan pakan diduplikasi sebanyak 3 ulangan, kemudian masing-masing diukur kecernaan bahan organik dan protein pada waktu fermentasi in vitro ke 2, 4, 6, 8, 12, 24 dan 48 jam. Data yang dikoleksi selanjutnya di uji regresi dan hasilnya dimasukkan dalam persamaan indeks SPE. Variabel yang diukur adalah indeks SPE. Hasil penelitian menunjukkan bahwa indeks SPE dedak sebesar 0,54, bekatul 0,50, pollard 0,57, jagung 0,87, gaplek 0,94, onggok kering 0,90 dan tepung roti 0,94. Penelitian menyimpulkan bahwa, konsentrat sumber energi memiliki potensi nilai indeks sinkronisasi protein-energi pada kategori sedang hingga tinggi dalam penyusunan ransum ruminansia. Jagung, gaplek, onggok kering dan tepung roti memiliki indeks sinkronisasi protein-energi pada kategori tinggi, sedangkan dedak, bekatul dan pollard pada kategori sedang.

scholarly journals Preservation Method of Rumen Fluid Collected From Harvested Cattle Alters in vitro Digestibility of Reference Feedstuffs

2021 ◽  
Vol 2 ◽  
Author(s):  
Taylor J. Garcia ◽  
Nichole M. Cherry ◽  
Kimberly A. Guay ◽  
Jeffrey A. Brady ◽  
James P. Muir ◽  
...  
Keyword(s):  
Rice Bran ◽  
Rumen Fluid ◽  
In Vitro Digestibility ◽  
Viable Option ◽  
Liquid Samples ◽  
Coastal Bermudagrass ◽  
Preservation Method ◽  
True Digestibility ◽  
Design Factors

The objective of our experiment was to evaluate preservation and revitalization strategies for rumen inoculum anticipating research and veterinary applications. Rumen fluid samples were collected from 12 harvested cattle. Liquid samples were divided into five 500-mL aliquots which were randomly allocated to one of five treatments in a 2 × 2 + 1 augmented factorial design. Factors included preservation method [freezing (FZN) or lyophilization (LYO)] and preservative (glycerol; + or –). A fresh control (CON) was maintained from each sample. Feedstuffs used in this experiment were alfalfa hay, Coastal bermudagrass hay, cracked maize, rice bran, and soybean meal. Reference feedstuffs were subjected to batch culture in vitro true digestibility (IVTD) and in vitro NDF digestibility (IVNDFD) assays using inoculum from each of the five treatments. There was an effect (P < 0.05) of preservation method, preservative, and their interaction for both IVTD and IVNDFD of each of the five references feedstuffs. Freezing or lyophilization of rumen inoculum reduced (P < 0.05) IVTD and IVNDFD of reference feeds relative to the CON. Despite lower degradation of feeds when frozen or lyophilized rumen fluid was used rather than fresh, differences between them in IVTD and IVNDFD suggest that, in the absence of fresh inoculum, preserved rumen fluid may be a viable option for veterinary applications, such as transfaunation, but likely will not be viable for research applications.

scholarly journals Synchronization Protein-Energy Index of Various Forages for Dairy Livestock: an In Vitro Study

2020 ◽  
Vol 22 (2) ◽  
pp. 92-97
Author(s):  
Afduha Nurus Syamsi ◽  
Hermawan Setyo Widodo
Keyword(s):  
Rumen Fluid ◽  
In Vitro Study ◽  
Coefficient Of Determination ◽  
In Vitro Techniques ◽  
Protein Energy ◽  
Degradation Rates ◽  
Medium Level ◽  
Energy Synchronization ◽  
High Level

The present study inventoried the protein-energy synchronization (PES) index of various types of grass. The study was conducted using in vitro techniques with material 5 types of grass (elephant, king, dwarf elephant, guinea, and setaria) and rumen fluid of the Friesian Holstein (FH). The PES index is calculated based on the average degradation of g protein and Kg organic matter (OM) per hour at the time of observation of 2nd, 4th, 6th, 8th, 12th, 24th, 48th, and 72nd hours. The hourly degradation rates were analyzed by linear regression to obtain the average hourly degradation rate. The results were included in the PES index calculation formula and discussed descriptively. This study found that all five grass had almost uniform nutritional value. Regression analysis results show a different equation, but with a high coefficient of determination (> 90%) on all types of grass. The PES index obtained in elephant, king, dwarf elephant, guinea, and setaria grass are 0.72, 0.66, 0.69, 0.58, and 0.68. The study concluded that elephant grass has a PES index at a high level, while king, dwarf elephant, guinea, and setaria grass at medium level for compiling dairy rations.

scholarly journals Evaluation of nutritive values and digestibility’s cacao( Theobroma cacao L.) pod husk fermented with lingzhi mushroom (Ganoderma lucidum) at different concentration and incubation time

2022 ◽  
Vol 951 (1) ◽  
pp. 012024
Author(s):  
T Hidayat ◽  
F Al-Majid ◽  
S Wajizah ◽  
Y Usman ◽  
Samadi
Keyword(s):  
Organic Matter ◽  
Incubation Time ◽  
Animal Feed ◽  
Rumen Fluid ◽  
Proximate Analysis ◽  
In Vitro Digestibility ◽  
Nutritive Values ◽  
Two Factors ◽  
Buffer Mixture

Abstract Cacao pod husk has been widely utilized as animal feed. The purpose of this study is to evaluate nutritive values and digestibility of fermented cacao pod husk with different concentration and incubation time. A completely randomized factorial design consisting two factors; lingzhi mushroom concentration (K1 = 7,5%; K2 = 15%) and incubation time (L1 = 15 d; L2 =30 d; L3 = 45 d) was employed in this study (n=3 replicates). Proximate analysis was performed to determine nutritive values of fermented cacao pod husk. Fermented samples were then subjected to an in vitro digestibility with rumen fluid and McDougall’s buffer mixture. The results of study reveal that cacao pod husk fermented with the concentration of 15% at 15d and 30 d significantly increased (P<0.05) crude protein content but not for other parameters. Dry and organic matter digestibility of cacao pod husk fermented with 7.5% of lingzhi mushroom at 45 d significantly improved (P<0.05) in vitro dry and organic matter of treatment. In conclusion, cacao pod husk fermented with different concentration and incubation time was able to improve the nutritive values and in vitro digestibility of cacao pod husk.

scholarly journals Volatile fatty acids and methane production in dairy cow ration based on protein-energy synchronization index with a meals protein source

2021 ◽  
Vol 31 (2) ◽  
pp. 114-120
Author(s):  
Afduha Nurus Syamsi ◽  
Lastriana Waldi
Keyword(s):  
Fatty Acids ◽  
Soybean Meal ◽  
Volatile Fatty Acids ◽  
Protein Source ◽  
In Vitro Techniques ◽  
Synchronization Index ◽  
Ch4 Production ◽  
Protein Energy ◽  
Energy Synchronization

The study was aimed to examine the production of volatile fatty acids (VFA) and methane (CH4) in rations based on protein-energy synchronization index (PES) using different meals protein sources (MPS). The study was conducted experimentally with in vitro techniques. The factorial completely randomized design (CRD) was used to examine six types of treatment rations originating from a combination of 3 PES index and two types of MPS, each repeated four times so that there were a total of 24 experimental units. The results showed that the interaction of the SPE index had no significant effect on the production of partial VFA, but had a significant effect on the acetate: propionate (A: P) ratio and CH4 production. The analysis of honestly significant different (HSD) results showed that the lowest A: P ratio and CH4 were found in R1. The orthogonal test shows that coconut and soybean meal had a quadratic effect on the A: P ratio and CH4. It concluded that the best PES index to optimize VFA and minimize CH4 production was at the index 0.6-0.61 with soybean meal as protein source supplementation.

scholarly journals 49 Effects of in-vitro digestibility in cannulated steers when supplemented different levels of glycerol

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 55-56
Author(s):  
Noheli Gutierrez ◽  
Jamie A Boyd
Keyword(s):  
Dry Matter ◽  
Cold Water ◽  
Rumen Fluid ◽  
Latin Square ◽  
Optimal Level ◽  
Nutrient Digestibility ◽  
In Vitro Digestibility ◽  
Study Results ◽  
Different Levels

Abstract A study was conducted to evaluate effects of increasing concentration of food grade glycerol on rumen environment and nutrient digestibility. Three ruminally cannulated Jersey steers were used in this study. The study was conducted from March to May 2019. Experimental design was a 3x3 Latin square with a 2wk adjustment period followed by a 1wk collection period. Diet was coastal bermudagrass hay based. Different forage types were introduced in the incubation process to evaluate digestibility. Glycerol was administered once a day at 0, 15, or 20% of DMI (dry matter intake). dNDF (digestible NDF) and dDM (digestible dry matter) was determined using an ANKOM Daisy II incubator inoculated with 200g fresh rumen fluid and incubated for 12, 24, 48 and 72 h at 39°C. Each vessel contained ground forage samples in filter bags in triplicate. After incubation, filter bags were rinsed with cold water and dried for 24h in a 55°C forced air oven. Data were analyzed using the Proc MIXED procedure of SAS version 9.4. There was no difference dNDF in effect of different levels of glycerol between forage types by diet. But a numerical tendency was observed that dNDF was decreased at 20% inclusion rates in comparison to 0 and 15% inclusion of glycerol in the diet. Neither steer nor run was significantly different in the study. However as expected digestibility over time was significantly different (P &lt; 0.001). A significant increase was observed in DMI with the increased levels of glycerol in the diet (P = 0.003), both the 15% and 20% levels of glycerol increased in DMI in comparison to the control (0%). It appears based on these study results that digestibility may be inhibited, as levels of dietary glycerol increase in the diet and more work needs to be done to find the optimal level of glycerol supplementation.

scholarly journals Produk Fermentasi Rumen dan Produksi Protein Mikroba Sapi Lokal yang Diberi Pakan Jerami Amoniasi dan Beberapa Bahan Pakan Sumber Energi

2011 ◽  
Vol 11 (2) ◽  
pp. 29-34 ◽  
Author(s):  
Novita Hindratiningrum ◽  
Muhamad Bata ◽  
Setya Agus Santosa
Keyword(s):  
Protein Synthesis ◽  
Rice Bran ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Gas Production ◽  
Metabolic Energy ◽  
Energy Sources ◽  
Microbial Protein ◽  
Microbial Protein Synthesis

Products of rumen fermentation and protein microbial of dairy cattle feed with rice bran ammonization and some feedstuffs as an energy sourcesABSTRACT. This study aims to examine the energy sources of feed ingredients that can increase the production of Volatile Fatty Acids (VFA), N-NH3, microbial protein synthesis, total gas production and metabolic energy. The material used is as a source of rumen fluid inoculum from Frisian Holstein cows (FH) females, amoniasi rice straw, salt, mineral mix brand "Ultra Minerals' production Eka Farma Semarang, onggok wet and dry, corn, and rice bran. Observed variable is the concentration of (VFA), N-NH3, rumen microbial protein synthesis, and total gas production. Based on the analysis of diversity seen any significant effect (P0.05) on total VFA concentration, N-NH3 and total gas but had no effect (P0.05) on microbial protein synthesis. Conclusion of research is the provision of energy sources with rice bran treatment, onggok wet and dry corn flour can be used as fermentable carbohydrates on feed hay amoniasi in vitro.

scholarly journals Nutrient Quality and in Vitro Digestibility of Bio-Ammoniated Rice Straw Bales

2020 ◽  
Vol 44 (3) ◽  
Author(s):  
Cuk Tri Noviandi ◽  
Dibya Ratnopama ◽  
Ali Agus ◽  
Ristianto Utomo
Keyword(s):  
Rice Straw ◽  
Crude Protein ◽  
Production Method ◽  
Proximate Analysis ◽  
Gas Production ◽  
Crude Fiber ◽  
In Vitro Digestibility ◽  
Nutrient Quality ◽  
Randomized Design

This study was done to determine the effects of bale sizes of bio-ammoniated rice straw on its nutrient quality and in vitro digestibility. Rice straw were bio-ammoniated by adding 2 g urea and 1 g probiotic/kg DM. By following a completely randomized design, rice straw was baled in 3 different weights (15, 25, and 35 kg) with six replications for each treatment, and then stored for 3 weeks. In the end of the week 3, bales were opened, aired, and then sampled for proximate analysis (dry matter, organic matter, crude protein, and crude fiber) and digestibility by in vitro gas production method. Using analysis of variance method, the proximate data showed that greater the bales size (15, 25, and 35 kg) increased crude protein (7.59, 7.86, and 9.95%, respectively; P<0.05) and decreased crude fiber contents (24.1, 22.1, and 18.8%, respectively; P<0.05). By increasing the size of bales also increased a, b, and c fractions (-0.79, 0.26, and 0.82 mL/100 mg DM; 20.2, 18.2, and 17.6 mL/100 mg DM; 0.012, 0.014, and 0.019 mL/h, respectively; P<0.05) as well as gas production (11.4, 11.5, and 13.8 mL/100 mg DM, respectively; P<0.05). It can be concluded that packing bio-ammoniated rice straw in 35 kg bale is the most effective way in increasing nutrient quality and digestibility of rice straw.

scholarly journals 190 Reduce Reuse ReRumen: Preservation method of rumen fluid collected from slaughtered cattle affects in vitro digestibility

2019 ◽  
Vol 97 (Supplement_1) ◽  
pp. 66-67
Author(s):  
Taylor J Garcia ◽  
Jeffrey A Brady ◽  
Kimberly A Guay ◽  
James P Muir ◽  
William B Smith
Keyword(s):  
Batch Culture ◽  
Rumen Fluid ◽  
Chemical Constituents ◽  
Rumen Content ◽  
In Vitro Digestibility ◽  
Constant Weight ◽  
Preservation Methods ◽  
Preservation Method ◽  
Pass Through

Abstract Microbes and chemical constituents in the rumen fluid play an important role when re-establishing the rumen microbial population. Identification of a viable preservation method could have lasting implication of veterinary and research applications. Our objective was to determine the efficacy of preservation methods on rumen liquor. Twelve paunch samples were collected from slaughtered cattle at the Tarleton State University Meat Laboratory, Stephenville, TX. Rumen content was collected from different locations within the rumen, strained through eight layers of cheesecloth, and divided into five 500-mL aliquots. Aliquots were randomly allocated to one of five preservation methods (freezing or lyophilisation) and preservative (glycerol; yes or no). A fresh control was maintained from each sample. Three reference feeds and two roughage sources were used in the batch culture procedure. Reference feeds were dried to a constant weight at 55°C and ground to pass through a 2-mm screen. Ground samples were subjected to batch culture in vitro true digestibility (IVTD) assays using each of the four ruminal fluids (plus the control) for digestibility estimates. Original samples and incubated residues were assayed for DM, CP, NDF, and ADF. Data were analyzed in a 2 × 2 + 1 augmented factorial design using GLIMMIX procedure of SAS. There were no differences (P > 0.05) in IVTD when using protein or energy feeds; however, there were differences in IVTD for all treatments for fiber concentrates and forage reference feeds. Despite lower degradation of feeds when frozen or lyophilized rumen fluid was used, preserved rumen fluid may be a viable option in the absence of fresh inoculum for veterinary and research applications

scholarly journals PENGARUH DAUN TURI (Sesbania grandiflora) DAN LAMTORO (Leucaena leucocephala) DALAM RANSUM SAPI BERBASIS INDEKS SINKRONISASI PROTEIN - ENERGI TERHADAP SINTESIS PROTEIN MIKROBA RUMEN

Pastura ◽  
2019 ◽  
Vol 6 (2) ◽  
pp. 47
Author(s):  
Afduha Nurus Syamsi ◽  
Fransisca Maria Suhartati ◽  
Wardhana Suryapratama
Keyword(s):  
Protein Synthesis ◽  
Rumen Fluid ◽  
Microbial Protein ◽  
In Vitro Techniques ◽  
Microbial Protein Synthesis ◽  
Sesbania Grandiflora ◽  
Protein Energy ◽  
Randomized Design ◽  
Better Than

An experiment was aimed to assess the use of the legume leaf as a source of protein feedstuff and levels of synchronization protein-energy (SPE) index in the diet of cattles on ammonia (N-NH3) and microbial protein synthesis (MPS). In vitro techniques was done. The research was used a completely randomized design (CRD), with factorially pattern (2x3), the first factor was the two species of legume (Sesbania leaves and Leucaena leaves) and the second factor was the three level of the SPE index (0.4, 0.5, and 0.6), there were 6 treatment combinations and each was 4 replicates. The results showed that no interaction between legume with SPE index, but each factor was significantly effect (P<0.05) on N-NH3 of rumen fluid and MPS. The research concluded that Leucaena leaf is a legume that is better than Sesbania leaf in terms of their ability toincrease MPS. SPE index is the best in producing MPS at level 0.6. Key words: Legume, synchronization of protein and energy index, ammonia, microbial protein synthesis

Differences between the in vitro digestibility of extrusa collected from oesophageal fistulated steers and the forage consumed

2009 ◽  
Vol 49 (7) ◽  
pp. 563 ◽  
Author(s):  
David B. Coates ◽  
Robert J. Mayer
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
In Vitro Digestibility ◽  
In Vitro Techniques ◽  
Pasture Legumes ◽  
Dry Matter Digestibility ◽  
Significant Difference ◽  
C3 Grasses

In a study that included C4 tropical grasses, C3 temperate grasses and C3 pasture legumes, in vitro dry matter digestibility of extrusa, measured as in vitro dry matter loss (IVDML) during incubation, compared with that of the forage consumed, was greater for grass extrusa but not for legume extrusa. The increase in digestibility was not caused by mastication or by the freezing of extrusa samples during storage but by the action of saliva. Comparable increases in IVDML were achieved merely by mixing bovine saliva with ground forage samples. Differences were greater than could be explained by increases due to completely digestible salivary DM. There was no significant difference between animals in relation to the saliva effect on IVDML and, except for some minor differences, similar saliva effects on IVDML were measured using either the pepsin–cellulase or rumen fluid–pepsin in vitro techniques. For both C4 and C3 grasses the magnitude of the differences were inversely related to IVDML of the feed and there was little or no difference between extrusa and feed at high digestibilities (>70%) whereas differences of more than 10 percentage units were measured on low quality grass forages. The data did not suggest that the extrusa or saliva effect on digestibility was different for C3 grasses than for C4 grasses but data on C3 grasses were limited to few species and to high digestibility samples. For legume forages there was no saliva effect when the pepsin–cellulase method was used but there was a small but significant positive effect using the rumen fluid–pepsin method. It was concluded that when samples of extrusa are analysed using in vitro techniques, predicted in vivo digestibility of the feed consumed will often be overestimated, especially for low quality grass diets. The implications of overestimating in vivo digestibility and suggestions for overcoming such errors are discussed.

Sign in / Sign up

Export Citation Format

Share Document