scholarly journals STUDY OF ACUTE AND SUB-ACUTE TOXICITY OF Boehmeria virgata (FORST) GUILL LEAF EXTRACT IN WISTAR RATS

2021 ◽  
Vol 9 (Spl-2-ICOPMES_2020) ◽  
pp. S248-S258
Author(s):  
Marianti Manggau ◽  
◽  
Endang Kusuma Intan ◽  
Maghfirah . ◽  
Wira Bahari Nurdin ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Acute Treatment ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Organ Weight ◽  
Control Group ◽  
Histopathological Study ◽  
Hydropic Degeneration ◽  
The People ◽  
Kidney Glomerulus

This study was carried out to evaluate the acute and sub-acute toxicity of the standardized Boehmeria virgata leaf extract (BLVE) in Wistar rats. B. virgata is a traditional herb utilized by the people of Makassar, Indonesia to cure inflammation and cancer. In the current study, thirty (30) Wistar rats were divided into 6 groups (3 groups of males and 3 groups of females) for acute treatments. Similarly, for investigating sub-acute treatment forty (40) Wistar rats were split into 8 groups (4 groups of males and 4 groups of females). For acute toxicity treatment, selected rats received 2000 mg and 5000 mg/kg of BVLE by oral administration for 14 days while for the estimation of sub-acute toxicity, experimental rats were treated with 250, 500, and 1000 mg/kg BVLE for 28 days. After 14 days of treatment, the rats were monitored for any form of changes in behavior, weight, food, water intake, and histopathology. The treated animals underwent hematological, biochemical, histopathological, and organ weight analysis after 28 days. Results of the study revealed no significant differences in body and organ weight, intake of food, and water after acute BVLE treatment in rats compared with those in the control group. The histopathological study suggested a general hydropic degeneration of the liver after acute treatment with 5000 mg/kg; such degeneration did not occur in the kidneys and kidney glomerulus of BVLE treated rats. Further, no significant toxicity was shown in hematological, biochemical, organ weight, and histopathological data in the sub-acute BVLE group after comparing treated rats with the control group. Results of the study can be concluded that BVLE is not toxic at doses of up to 1000 mg/kg. Therefore B. virgate fulfilled a preclinical criterion that is necessary for its further establishment as a clinically useful extract.

scholarly journals Effect of Ethanol Leaf Extract of Khat (Catha edulis forsk) on Prefrontal Cortex Oxidative Markers and Hematological Profile of Wistar Rats

2021 ◽  
pp. 149-162
Author(s):  
Solomon Adomi Mbina ◽  
Sanusi Bello Mada ◽  
Ejike Daniel Eze ◽  
Clement Olusoji Ajayi ◽  
Josiah Eseoghene Ifie ◽  
...  
Keyword(s):  
Prefrontal Cortex ◽  
Acute Toxicity ◽  
Study Design ◽  
Crude Extract ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Lethal Dose ◽  
Control Group ◽  
Catha Edulis ◽  
Oxidative Markers

Aim: This study evaluated the effect of ethanol leaf extract of Catha edulis on the prefrontal cortex oxidative markers and hematological profile of Wistar rats. Study Design: The study design was experimental. Place and Duration of Study: Department of Biochemistry, Kampala International University, Uganda and Faculty of Medicine, Department of Pharmacy, Mbarara University of Science and Technology, Mbarara, Uganda. Between January and May, 2021. Methodology: Fresh C. edulis leaves were collected after on-spot identification, and authenticated before being chopped into small pieces and pulverized mechanically. The powdered material was extracted through cold maceration using 70% ethanol for 72 h. The extract was concentrated in vacuo to dryness at 40oC using rotary evaporator. The lethal dose (LD50) of the crude extract was determined using acute toxicity test. The sub-acute toxicity of the crude extract was evaluated by orally administering daily doses of 250, 500, and 1000 mg/kg to the male Wistar rats for 28 days. The animals were monitored for mortality, behavioral and physiological signs of toxicity. Superoxide dismutase (SOD), Catalase (CAT) activities and Malondialdehyde (MDA) levels were estimated using standard methods. The blood samples were analyzed using the automated hematology analyzer: Mindray BC-6000. Shenzhen, China. Results: The median lethal dose (LD50) of the C. edulis leaf extract was greater than 5000mg/kg b w. The SOD and CAT activities of C. edulis treated groups compared to the control group were significantly (p < 0.05) reduced, the malondialdehyde (MDA) levels were increased in C. edulis treated groups compared to the control group. There was no significant change in the hematological parameters, however, there were slight variations in the WBC and RBC counts in some C. edulis treated groups. Conclusion: Long term chewing of C. edulis could result in oxidative stress. It is therefore important for chronic C. edulis leaf chewers to take caution.

acute and sub-acute toxicity studies of aqueous leaf extract of launaea taraxacifolia (willd.) in wistar rats

2021 ◽  
Vol 12 (1) ◽  
pp. 95
Author(s):  
Millicent Umaru ◽  
Kabiru Abubakar ◽  
Gabriel Uyaiabasi ◽  
Giaze Tijjani ◽  
Yusuf Alkali ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Toxicity Studies ◽  
Aqueous Leaf Extract

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals Histopathological Assessment of the Effect of Pregabalin Toxicity on Cerebrum and Cerebellum of Adult Albino Rats

2021 ◽  
Author(s):  
Wael Abdou Hassan ◽  
Shaimaa Shehata ◽  
Ahmad ElBana
Keyword(s):  
Cerebral Cortex ◽  
Acute Toxicity ◽  
Chronic Toxicity ◽  
Control Group ◽  
Albino Rats ◽  
Histopathological Study ◽  
Addictive Behaviors ◽  
Group 3 ◽  
Group 2 ◽  
Cerebral Cortex Tissue

Abstract Background: Pregabalin (PGB) used as analgesic in treatment of neurogenic pains of chronic diseases, is considered as one of the most abused anti-epileptic drugs worldwide and it has been proved that it induces addictive behaviors. The present histopathological study aimed to identify the effect of PGB administration on cerebral cortex and cerebellar cortex, in both acute and chronic toxicity. Seventy-two male and non-pregnant female adult albino rats’ 6- to 8-week-old divided into 3 main groups of 24 rats each were studied. Group 1 represented the control group and group 2 represented the acute toxicity group, in which rats were given a single dose of PGB (5000 mg/kg) orally by gavage and after 24 hours, rats were sacrificed and examined. Group 3 represented the chronic toxicity group; were given PGB 500 mg/kg orally by gavage for 12 weeks, after which rats were sacrificed and examined. Result: Cerebral cortex tissue of acute toxicity group displayed astrocytosis and dystrophic changes, while in chronic group showed degeneration, necrosis and cellular infiltrates. The cerebellum of chronic groups showed degeneration and shrunken of Purkinje cells. Conclusion: Acute and chronic intoxication with pregabalin adversely altered the structure of cerebral cortex and cerebellum.

scholarly journals GAMBARAN HISTOLOGIK AORTA TIKUS WISTAR DENGAN DIET LEMAK BABI SETELAH PEMBERIAN EKSTRAK DAUN PEPAYA

2013 ◽  
Vol 5 (1) ◽  
Author(s):  
Friska W. F. Panjaitan ◽  
Marie M. Kaseke ◽  
George N. Tanudjaja
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Foam Cells ◽  
Group Iv ◽  
Control Group ◽  
Histological Features ◽  
Group I ◽  
Group Ii ◽  
Lard Diet ◽  
Control Group Group

Abstract: Papaya leaves contain several antioxidants (flavonoid, tanin, and vitamin C) which have antiatherogenic effects that may inhibit the progression of an atherosclerotic lesion. One of the risk factors causing atherosclerosis is the consumption of food containing highly saturated fatty acids e.g. lard. Low density lipoproteins (LDL) accumulate within the intima and then are oxidized (LDL-ox). This LDL-ox is ingested by macrophages, resulting in foam-cell formation (early lesion of atherosclerosis). This study aimed to find out the histological features of the aorta of wistar rats having lard diets without the addition of the papaya leaf extract; having lard diets along with the papaya leaf extract; and having lard diets followed by papaya leaf extract. This was an experimental study on 16 wistar rats divided into 4 groups: group I without treatment (negative control group), group II lard diet for 14 days (positive control group), group III lard diet with papaya leaf extract for 14 days (treatment group I), and group IV lard diet for 14 days, and then followed by papaya leaf extract for 14 days (treatment group II). It was found that the aorta of group I showed adipose cells in the intima and media layers; group II and III showed foam cells in both layers; and group IV showed foam cells in fewer numbers than group II. Conclusion: The aorta histological features of wistar rats given lard diets for 14 days, with or without papaya leaf extract, showed foam cells in the intima and media layers. Papaya leaf extraxt added to lard diets had no effect on decreasing foam cells (no protective effect), meanwhile papaya leaf extract following lard diets showed a reduction of foam cells (therapeutic effect). Keywords: papaya leaf, lard dietary, foam cells, wistar rat.   Abstrak: Daun pepaya mengandung antioksidan (flavonoid, vitamin C) yang berefek anti-aterogenik, sehingga diharapkan dapat menghambat perkembangan lesi aterosklerosis. Salah satu faktor risiko penyebab aterosklerosis yaitu makanan yang berkandungan tinggi asam lemak jenuh, antara lain lemak babi. Konsumsi lemak jenuh berlebihan dapat mengganggu fungsi sel endotel, sehingga lipoprotein berdensitas rendah (LDL) dapat masuk dan menjadi LDL teroksidasi (LDL-oks). Makrofag menangkap LDL-oks dan menjadi sel busa (lesi dini aterosklerosis). Penelitian ini bertujuan untuk mengetahui gambaran histologik aorta tikus wistar dengan diet lemak babi tanpa pemberian ekstrak daun pepaya, diet lemak babi bersamaan pemberian ekstrak daun pepaya, dan setelah diet lemak babi dilanjutkan pemberian ekstrak daun pepaya. Penelitian ini bersifat eksperimental. Subyek penelitian terdiri dari 16 ekor tikus wistar yang dibagi menjadi empat kelompok: kelompok I tanpa perlakuan (kelompok kontrol negatif); kelompok II dengan diet lemak babi selama 14 hari (kelompok kontrol positif); kelompok III dengan diet lemak babi serta pemberian ekstrak daun pepaya selama 14 hari (kelompok perlakuan I); dan kelompok IV dengan diet lemak babi selama 14 hari, dilanjutkan pemberian ekstrak daun pepaya selama 14 hari (kelompok perlakuan II). Hasil penelitian memperlihatkan gambaran histologi aorta kelompok I tampak perlemakan; pada kelompok II terdapat sel-sel busa; pada kelompok III masih terdapat sel-sel busa; dan pada kelompok IV terdapat sel-sel busa, namun dalam jumlah yang lebih sedikit dibandingkan pada gambaran kelompok II. Simpulan: Tikus wistar dengan diet lemak babi selama 14 hari, baik dengan maupun tanpa ekstrak daun pepaya, memperlihatkan gambaran histologik adanya sel-sel busa pada tunika intima dan tunika media aorta. Pemberian ekstrak daun pepaya bersamaan dengan diet lemak babi tidak berefek menurunkan jumlah sel busa (tidak ada efek protektif) sedangkan pemberian ekstrak daun pepaya setelah diet lemak babi berefek mengurangi jumlah sel-sel busa yang terbentuk (efek terapi). Kata kunci: daun pepaya, diet lemak babi, sel-sel busa, tikus wistar.

scholarly journals THE COMPARISON OF RAMANIA (Bouea macrophylla Griff) AND BINJAI (Mangifera caesia) LEAVES EXTRACT GEL EFFECT ON COLLAGEN DENSITY

2021 ◽  
Vol 6 (2) ◽  
pp. 159
Author(s):  
Nabilah Nabilah ◽  
Irham Taufiqurrahman ◽  
Nolista Indah Rasyid
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Total Sample ◽  
Control Group ◽  
Control Group Design ◽  
Gel Effect ◽  
Index Measurement ◽  
Male Wistar Rats ◽  
Collagen Density ◽  
Post Hoc

Background: The extract of ramania Bouea macrophylla Griff) and binjai (Mangifera caesia) leaf have flavonoid compounds that function as antioxidants to balance the amount of Reactive Oxygen Species (ROS) in tissues and optimize wound healing by helping synthesis of hydroxyproline which is used as a collagen synthesis material. Objective: To compare the effect of 15% concentration of ramania leaf extract gel, 15% concentration of binjai leaf extract gel on collagen density in back incision wounds of male Wistar rats on day 7 and day 14. Methods: This study used a true experimental design with a posttest-only with control group design. The study sample used male wistar rats that were healthy and active, aged 2-3 months with a body weight of 250-300 grams. The total sample was 18 rats divided into 6 groups. The 15% concentration of ramania and binjai leaves was given topically, then the rats were euthanized on the 7th and 14th day. Collagen index measurement was using hydroxyproline concentration. Results: Two-way Anova data analysis showed a significant value of 0.00 (p<0.05), which means that there was a difference in effect between the treatment gel and wound day. Bonferroni Post Hoc test showed a significant value in all treatment gel groups. Conclusion: There is a difference in the effect of 15% concentration of ramania leaf extract gel and 15% concentration of binjai extract gel on collagen density. Binjai leaf extract gel at 15% concentration is more effective for collagen density than Ramania leaf extract at 15% concentration and placebo. Keywords : Binjai Leaf Extract Gel, Collagen, Hydroxyproline, Ramania Leaf Extract Gel.

scholarly journals Hypoglycemic and Toxic Effect of Morus mesozygia Leaf Extract on the Liver and Kidneys of Alloxan-Induced Hyperglycemic Wistar Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Michael Tirwomwe ◽  
Isaac Echoru ◽  
Richard Maseruka ◽  
Kyobe Ronald Kimanje ◽  
Wilson Byarugaba
Keyword(s):  
Toxic Effect ◽  
Total Protein ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Statistical Significance ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Histological Damage ◽  
Alloxan Monohydrate ◽  
Antidiabetic Effects

Purpose. We investigated the hypoglycemic and toxic effect of Morus mesozygia leaf extract on the liver and kidneys of alloxan-induced hyperglycemic wistar rats. Method. Phytochemical analysis was done. Diabetes was induced by the use of alloxan monohydrate in six groups of rats, i.e., 200 mg/kg, 400 mg/kg, 800 mg/kg, glibenclamide, normal saline, and normal control group. Blood glucose was measured at the time of inoculation, then at 1, 2, 3, and 4 hours after. After 14 days, rats were killed under anesthesia; blood collected for measurement of total protein, albumin, TAGs, cholesterol, AST, ALT, urea, and creatinine; and whole tissue of liver and kidneys used for histological studies. Results. The extract possessed antidiabetic effects between 400 mg/kg and 800 mg/kg doses, which we attributed to the presence of flavonoids, tannins, terpenoids, and amino acids. There was a drop in total protein and albumin with no statistical significance (P≥0.05). The changes in levels of ALT, TAGs, cholesterol, AST, creatinine, and urea were not statistically different from the standard diabetic drug. The extract was protective against histological damage as there were no significant lesions suggestive of toxicities in the liver and kidneys at doses below 800 mg/kg. Conclusion. We established credible evidence that Morus mesozygia leaf extract has hypoglycemic effects between 400 mg/kg and 800 mg/kg and that it is safe on the liver and kidneys of wistar rats at doses less than 800 mg/kg.

scholarly journals The Effect of Ethanolic Extract of Myrmecodia pendans on Gentamicin Induced Nephrotoxicity in Wistar Rats

2021 ◽  
Vol 10 (2) ◽  
pp. 96-101
Keyword(s):  
Serum Creatinine ◽  
Wistar Rats ◽  
Ethanolic Extract ◽  
Control Group ◽  
Hydropic Degeneration ◽  
Tubular Necrosis ◽  
Vascular Congestion ◽  
Nephroprotective Activity ◽  
The Mean ◽  
Nephroprotective Effect

The purpose of the study was to investigate the nephroprotective effect of the Myrmecodia pendans extract on Wistar rats. The nephrotoxicity of the animals was induced through intra-peritoneal administration of 100mg/kg/day of gentamicin for ten days. The effect of M. pendans extract at a dose of 250mg/kg/day was concurrently monitored in some rats by assessing their serum creatinine levels, blood urea nitrogen (BUN) and the histopathological parameters. The mean of serum creatinine in control group was 1.17±0.18mg/dl and in nephrotoxic group it was 2.22±0.29 mg/dl, while in group given the extract were 1.38±0.19 and 1.27±0.15mg/dl, respectively. In that group also sequentially showed BUN levels of 24.27±2.6, 108.13±11.58, 30.70±4.23 and 27.28±2.84mg/dl, respectively. Nephrotoxicity was induced in the animals injected with gentamicin, which showed a significant (P<0.05) increase in creatinine and BUN compared with control group. The results also showed that the M. pendans ethanolic extracts significantly (P<0.05) prevented the increase in the levels of serum creatinine, BUN and reduced renal histopathological damage such as hydropic degeneration, vascular congestion and tubular necrosis. Therefore, the ethanolic extract of M. pendans exhibits a nephroprotective activity.

scholarly journals Uji Efek Analgesik Ekstrak Daun Jarak Pagar (Jatropha Curcas L.) terhadap Tikus Wistar (Rattus Norvegicus)

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Oras B.M. Yensenem ◽  
Jane Wuisan ◽  
Henoch Awaloei
Keyword(s):  
Jatropha Curcas ◽  
Rattus Norvegicus ◽  
Analgesic Effect ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Negative Control ◽  
Control Group ◽  
Tropical Country ◽  
Mean Values ◽  
Jatropha Curcas L

Abstract: Indonesia is a tropical country with the second largest biodiversity in the world after Brazil. Jatropha curcas (Jatropha curcas L.) was believed as a pain reliever (analgesic). This study was aimed to prove the analgesic effect of Jatropha curcas leaf extract on Wistar rats (Rattus norvegicus). This was an experimental study using hot stimulus method in the form of temperature of 65°C. Subjects were 15 male Wistar rats divided into 5 groups. Group 1, the negative control group; group 2, the positive control group given paracetamol 40 mg/kgBW; and 3 treatment groups (groups 3, 4, and 5) given Jatropha curcas extract in several doses (300 mg/kgBW, 600 mg/kgBW, and 1200mg/kgBW). Observation of Wistar rat respons (licking and jumping) was performed for 1 minute at minutes -30, 60, 90, and 120 after treatment. The results showed that the mean values of response against heat decreased in accordance to time and reached the lowest values at minute 120, except group 5 (the extract dose of 1200mg/kg BW) which reached the lowest value at minute 90. Overall, the lowest mean value was found in group 4 (the extract dose of 600mg/kg BW). Conclusion: Jatropha curcas (Jatropha curcas L.) leaf extract had an analgesic effect on Wistar rats (Rattus norvegicus).Keywords: Jatropha curcas L., Rattus norvegicus, analgesic Abstrak: Indonesia merupakan negara beriklim tropis dengan keaneka ragaman hayati terbesar kedua di dunia setelah Brazil. Penelitian ini bertujuan untuk mengetahui efek analgesik pemberian ekstrak daun jarak pagar (Jatropha curcas L.) pada tikus Wistar (Rattus norvegicus). Jenis penelitian ini ialah eksperimental dengan mengguna-kan metode rangsang panas berupa suhu 65°C. Subjek penelitian mengunakan hewan uji berupa tikus Wistar (Rattus norvegicus) jantan sebanyak 15 ekor yang dibagi menjadi 5 kelompok, yaitu: Kelompok kontrol negatif; kelompok kontrol positif yang diberikan parasetamol 40 mg/kgBB; dan 3 kelompok perlakuan yang diberikan ekstrak jarak pagar (Jatropha curcas L.), dengan dosis 300 mg/kgBB, 600 mg/kgBB, dan 1200mg/kgBB. Pengamatan terhadap respon tikus Wistar berupa jilat dan lompat dilakukan selama 1 menit pada menit ke-30, 60, 90, dan 120 setelah pemberian ekstrak jarak pagar. Hasil penelitian mendapatkan bahwa terdapat penurunan nilai rerata respons terhadap panas yang sesuai dengan perlangsungan waktu kecuali pada kelompok 5 (dosis ekstrak daun jarak pagar 1200mg/kg BB) yang mencapai nilai terendah pada menit ke-90. Secara keseluruhan, nilai rerata respons yang terendah didapatkan pada kelompok 4 (dosis ekstrak daun jarak pagar 600mg/kg BB) pada menit ke-120. Simpulan: Ekstrak daun jarak pagar (Jatropha curcas L.) memiliki efek analgesik terhadap tikus Wistar (Rattus norvegicus)Kata kunci: Jatropha curcas L., Rattus norvegicus, analgesic

scholarly journals PENGARUH EKSTRAK DAUN GEDI (Abelmoschus manihot L.) TERHADAP GAMBARAN HISTOPATOLOGI AORTA TIKUS WISTAR DENGAN DIET ATEROGENIK

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Nadya Nadiratika Papodi
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Foam Cells ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Blood Cholesterol ◽  
Control Group ◽  
Pork Lard ◽  
Pork Fat

Abstract: High blood cholesterol is a major risk factor for atherosclerosis. Gedi plants contain polyphenolic compounds that can lowering blood cholesterol levels. This study aimed to find out the effect of gedi (Abelmoschus manihot L) leaves extract on histopatologic features of aorta of those wistar rats, which had been administered an atherogenic diet. This was an experimental study on animal using 20 Wistar rats as samples. The rats were divided into 4 different groups, 5 rats for each group. Negative control group was fed only pelet, positive control group was fed pork fat as much as 2 ml/day, group C was fed on pork fat and gedi leaf extract, and group D was fed on pork fat then continued with gedi leaf extract. The results indicated that administration of 30 mg gedi leaf extract showed a histopathologic feature which is decreasing of foam cells. Conclusion: Microscopically, aorta of those rats fed on pork lard diet showed a number of foam cells in tunica intima and media whereas the rats that were fed on pork lard along with gedi leaf extract, generally there were no foam cells, but there aresome parts which contain few foam cells. On the group of Wistar rats which were fed on pork lard, continued with gedi leaf extract showed less foam cells compared to the positive control group.Keywords: Gedi leaf extract, pork lard, foam cell.Abstrak: Kadar kolesterol darah yang tinggi merupakan faktor risiko utama terjadinya aterosklerosis. Tumbuhan gedi mengandung senyawa polifenol yang dapat menurunkan kadar kolesterol darah. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak daun gedi terhadap gambaran histopatologi aorta tikus wistar dengan diet aterogenik. Hewan uji yang digunakan berupa 20 ekor tikus Wistar yang dibagi ke dalam 4 kelompok, masing-masing kelompok terdiri dari 5 ekor tikus. Kelompok kontrol negatif hanya diberikan pelet biasa, kelompok kontrol positif diberikan diet lemak babi 2ml/hari, kelompok C diberikan diet lemak babi bersamaan dengan ekstrak daun gedi, dan kelompok D diberikan lemak babi kemudian dilanjutkan dengan pemberian ekstrak daun gedi. Hasil penelitian memperlihatkan bahwa pemberian ekstrak daun gedi 30 mg mampu memberikan gambaran histopatologi aorta berupa berkurangnya jumlah sel busa. Simpulan: Secara mikroskopik, aorta tikus wistar yang diberi lemak babi menunjukkan adanya penumpukan sel-sel busa pada tunika intima dan media, sedangkan pada aorta tikus wistar yang diberi lemak babi bersamaan dengan ekstrak daun gedi maupun yang dilanjutkan dengan ekstrak daun gedi ditemukan berkurangnya jumlah sel-sel busa.Kata Kunci : Ekstrak daun gedi, Lemak babi, Sel busa.

Sign in / Sign up

Export Citation Format

Share Document