scholarly journals Association between polymorphism at the promotor region of NO-synthase gene and bronchial asthma development

2007 ◽  
pp. 52-55
Author(s):  
I. V. Petrova ◽  
D. V. Kozyritskaya ◽  
E. M. Kamaltynova ◽  
L. M. Ogorodova
Keyword(s):  
Bronchial Asthma ◽  
Molecular Genetic ◽  
No Synthase ◽  
Functional Characteristics ◽  
Basic Therapy ◽  
Asthmatic Children ◽  
Inherited Predisposition ◽  
Promotor Region ◽  
Children With Asthma ◽  
Polymorphic Variants

The purpose of this work was to study NO-synthase gene polymorphic variants in term of asthma occurrence. Clinical and functional characteristics of asthma with regards to different NO-synthase gene polymorphic variants were analyzed in 250 asthmatic children aged 7 to 14 years under the 12-wk standard basic therapy for asthma. We used the typical spectrum of tests for children with asthma and molecular genetic methods. Polymorphism at the promotor region of the NO-synthase gene was found to be associated with phenotype of pathogenic features of asthma and was an important part of inherited predisposition to asthma.

THE RELATIONSHIP BETWEEN PARENTAL AND CHILDREN'S SERUM IMMUNOGLOBULIN E (IgE) AND ASTHMA

PEDIATRICS ◽  
1996 ◽  
Vol 98 (2) ◽  
pp. 329-329
Author(s):  
Stanley P. Galant
Keyword(s):  
Bronchial Asthma ◽  
Immunoglobulin E ◽  
Skin Tests ◽  
Z Score ◽  
Significant Determinant ◽  
Positive Skin ◽  
Asthmatic Children ◽  
Children With Asthma ◽  
Z Scores ◽  
The Relationship

Purpose of the Study. To examine parental factors that influence bronchial asthma and immunoglobulin E (IgE) levels in their children. Methods. The data in this study were derived from the Tucson Epidemiological Study of Airways Obstructive Diseases since 1972. Eleven evaluation periods have been performed since that time. Asthma histories were established by questionnaire. IgE levels were obtained in 738 children, 1043 fathers, and 1261 mothers. IgE levels were determined by paper radioimmunosorbent (PRIST) technique. IgE Z scores were established. An IgE Z score is the number of standard deviations IgE differs from matched age and sex-matched subjects and log corrected. Skin tests were performed by prick technique to house dust, mold, grass, tree and weed mix and appropriate controls. Results. The percentage of children with bronchial asthma increased with the presence of asthma in parents so that 11.5% of children with asthma occurred in families with no parental asthma compared with 48.6% when both parents had asthma. The rate of childhood bronchial asthma was significantly related to parental IgE levels only when the mother and father had bronchial asthma (43% vs 20%) and much less so (22% vs 10%), when there was no parental asthma. In addition, asthma in the child proved to be a highly significant determinant of the child's IgE Z score, even after correcting for parental IgE Z score. The presence of atopy (ie, positive skin tests) was also not a significant determinant of IgE levels in asthmatic children. Discussion. The authors conclude that the inheritance of IgE is only one factor related to the development of asthma, and is limited as a predictor.

scholarly journals ROLE OF GENETIC PREDISPOSITION, GENE POLYMORPHISM OF GLUTATHIONE-S-TRANSFERASE (GSTT1, GSTM1, GSTP1) AND SOME ADVERSE FACTORS IN DEVELOPMENT OF BRONCHIAL ASTHMA IN CHILDREN – RESIDENTS OF RADIOACTIVELY CONTAMINATED AREAS

2021 ◽  
Vol 26 ◽  
pp. 449-463
Author(s):  
Ye. Stepanova ◽  
◽  
I. Kolpakov ◽  
V. Vdovenko ◽  
V. Zigalo ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Bronchial Asthma ◽  
Gene Deletion ◽  
Molecular Genetic ◽  
Glutathione S Transferase ◽  
Genetic Studies ◽  
Hereditary Predisposition ◽  
Gstm1 Gene ◽  
Polymorphic Variants ◽  
Asthma In Children

Objective: to determine the influence of hereditary predisposition, polymorphism of GSTT1, GSTM1, GSTP1 genes and environmental factors on the development of bronchial asthma in children – residents of radioactively contaminated areas. Materials and methods. School-age children-residents of radioactively contaminated areas with bronchial asthma, and those without clinical signs of respiratory pathology were examined. Genetic, medical, biological and social risk factors were determined based on the study of anamnestic data and medical records. Ventilation lung capacity was assessed by the method of computer spirometry. Molecular genetic studies were carried out using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) for further analysis. Results. Molecular genetic studies of the distribution of genotypes and frequencies of polymorphic variants of the genes GSTT1, GSTM1, GSTP1 were performed in children living under long-term intake of 137Cs by food chains. It was found that in children with BA the tendency to frequency of the deletion variant of the GSTT1 and GSTM1 genes in comparison with children without bronchial and pulmonary pathology was increased. The study of distributing the GSTP1 A313G gene polymorphic variants revealed in children with BA a significant increase in the frequency of AG-genotype, compared with the data of reference group. Adverse factors that increase the risk of developing bronchoobstructive disorders and the probability of their implementation in the form of bronchial asthma in children residents of RCA have been identified. It is established that among them the leading role is played by hereditary predisposition to this disease. On the part of the child, such negative factors were unfavorable conditions of fetal development, the presence of signs of exudative-catarrhal diathesis, manifestations of allergies and frequent respiratory diseases from the first months of life. It was found that the risk of developing BA was significantly increased in children with the GSTT1 and GSTM1 gene deletion genotypes; an increased risk of developing BA in children with a combination of the GSTP1 A313G gene polymorphism with deletion polymorphism of the GSTT1 or GSTM1 gene was determined. Сonclusion. Оne of the leading mechanisms, due to which there is a realization of hereditary predisposition to bronchial asthma in children living under constant intake of radionuclides with a long half-life, is the polymorphism of certain glutathione-S-transferase genes, namely, GSTT1, GSTM1 and A313G gene deletion polymorphism and GSTP1 gene polymorphism. Key words: children, radioactively contaminated areas, risk factors, bronchial asthma, glutathione-S-transferase gene polymorphism.

Chronic Low-Grade Systemic Inflammation in Children with Asthma and Obesity

2018 ◽  
Vol 11 (2) ◽  
pp. 128-134
Author(s):  
Niya A. Krasteva ◽  
Boiko R. Shentov ◽  
Adelaida L. Ruseva ◽  
Chaika K. Petrova ◽  
Simeon P. Petkov
Keyword(s):  
Bronchial Asthma ◽  
Systemic Inflammation ◽  
Inflammatory Markers ◽  
Serum Levels ◽  
Control Group ◽  
Low Grade ◽  
Obese Children ◽  
Asthmatic Children ◽  
Children With Asthma ◽  
Hs Crp

Summary The rising incidence of bronchial asthma and obesity in children raises the question of whether there is a link between them. Chronic low-grade systemic inflammation could be one of the linking mechanisms. We aimed to determine the serum concentrations of high-sensitive C-reactive protein (hs-CRP), interleukin 6 (IL-6) and tumour necrosis factor a (TNF-a) in children with asthma and obesity and to seek a relationship between these inflammatory markers and asthma control. We investigated 88 children aged 6 to 17 years - 25 asthmatic obese children (AsOb), 25 asthmatic non-obese children (AsNOb), 19 obese non-asthmatic children (ObNAs), and 19 non-obese non-asthmatic children as controls. Serum levels of IL-6 and hs-CRP were significantly increased in asthmatic obese and ObNAs compared to AsNOb and the control group. Serum TNF-a concentration was similar in the four studied groups. There were no statistically significant differences in serum levels of these inflammatory markers between controlled and partially controlled/uncontrolled asthmatics (obese and non-obese). Knowing the possible mechanisms of interaction between bronchial asthma and obesity would contribute to a more effective therapeutic approach in these patients.

Psychometric Testing and Refinement of the Support Needs Inventory for Parents of Asthmatic Children

2004 ◽  
Vol 12 (3) ◽  
pp. 179-193
Author(s):  
Christine Toye ◽  
Linda J. Kristjanson ◽  
Mardhie E. Coleman ◽  
Hendrika Maltby ◽  
Glenda Jackson
Keyword(s):  
Factor Analysis ◽  
Internal Consistency ◽  
Psychometric Testing ◽  
Internal Consistency Reliability ◽  
Support Needs ◽  
Clinical Screening ◽  
Asthmatic Children ◽  
Children With Asthma ◽  
Undue Burden ◽  
Over Time

This study refined the Support Needs Inventory for Parents of Asthmatic Children (SNIPAC) (Coleman, Maltby, Kristjanson, & Robinson, 2001) to produce a more parsimonious tool to assess the importance and meet the support needs of parents of children with asthma. The original tool was completed by 145 parents of 199 children with asthma, and 74 of these also provided test-retest responses. Internal consistency reliability, construct validity, and stability over time were assessed and refinements were made. Internal consistency reliability of the revised 20-item tool ranged from .77 to .95 for the three subscales of the Parent’s Priority Scale (PPS), and .92 for the full PPS. Cronbach’s alphas ranged from .74 to .90 for the three subscales of the Parent’s Fulfillment Scale (PFS) and was .91 for the full scale. Factor analysis results of the PPS were compatible with the tool’s conceptual framework. The revised 20-item tool demonstrated adequate psychometric properties in most areas. This tool may be used for research or clinical screening without imposing undue burden on parents. Further work is required to establish the tool’s stability over time.

Phenotypes of professional bronchial asthma from the standpoint of spirometry

2021 ◽  
pp. 43-54
Author(s):  
Antonina G. Baykova ◽  
Marina Yuryevna Vostroknutova ◽  
Natalia A. Ostryakova ◽  
Tatyana Mikhailovna Kiryushina
Keyword(s):  
Comparative Analysis ◽  
Bronchial Asthma ◽  
Clinical Stage ◽  
Molecular Genetic ◽  
Control Group ◽  
Forced Exhalation ◽  
Choice Of Treatment ◽  
Genetic Examination

The aim of the study was to conduct a comparative analysis of spirometric indicators of respiration in various phenotypes of occupational bronchial asthma. Materials and methods. At the clinical stage of the work, a comprehensive clinical, radiological, spirographic, echocardiographic, immunological and molecular genetic examination of 170 patients of the main groups and 50 individuals of the control group was carried out. The results of the study. Dynamic determination of the speed indicators of forced exhalation in various phenotypes of occupational bronchial asthma can improve the diagnosis of obstructive disorders in this pathology, optimize the choice of treatment tactics, and predict the course of this pathology.

scholarly journals The Effects of Climate Therapy on Cardiorespiratory Fitness and Exercise-Induced Bronchoconstriction in Children with Asthma

Atmosphere ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1486
Author(s):  
Carlo De Pieri ◽  
Michele Arigliani ◽  
Maria Pia Francescato ◽  
Maurizio Droli ◽  
Michael Vidoni ◽  
...  
Keyword(s):  
Urban Area ◽  
Positive Impact ◽  
Asthmatic Children ◽  
Children With Asthma ◽  
Z Scores ◽  
Exercise Induced ◽  
Run Test ◽  
Age Range ◽  
Cardiorespiratory Endurance ◽  
Shuttle Run

We investigated whether a 1-week stay in the mountains may have a positive impact on Exercise-Induced Bronchoconstriction (EIB) and cardiorespiratory endurance in asthmatic children from an urban area. Spirometry was performed before and 10 min after a 20 m shuttle run test (20mSRT) on the first and seventh day of a summer asthma camp in the Italian Alps at 900 m of altitude. Spirometry z-scores were derived from the Global Lung Initiative 2012 prediction equations, and percentiles of the 20mSRT performance were assigned according to De Miguel-Etayo’s and Tomkinson’s predictive equations. A FEV1 decrease ≥10% after the exercise was defined as EIB. Particulate matter pollution was monitored during the camp and in the urban area of provenience. Twenty-four subjects (age range 7–16 years) were included. Frequency of EIB decreased from 58% (14/24) at day-1 to 33% (8/24) at the end of the camp (p = 0.08). Most subjects with a 20mSRT in the lowest quartile at day 1 had EIB (9/11). The proportion of children with a 20mSRT <25° percentile decreased from 45% (11/24) at day-1 to 16% (4/24) at day-7 (p = 0.02). Conclusion: One-week climate therapy in the mountains improved both bronchial hyperreactivity and cardiorespiratory endurance in our cohort of asthmatic children.

scholarly journals Correlation between vitamin D serum level and total IgE in asthmatic children

2020 ◽  
Vol 12 (2) ◽  
pp. 37-45
Author(s):  
Vesna Micevska ◽  
Tatjana Jakovska Mareti ◽  
Ilija Kirovski ◽  
Olivera Jordanova
Keyword(s):  
Vitamin D ◽  
Serum Level ◽  
P Value ◽  
Healthy Children ◽  
Total Ige ◽  
Asthmatic Children ◽  
Significant Difference ◽  
Children With Asthma ◽  
The Mean ◽  
Chronical Disease

Asthma is a chronical disease of the airways characterized by reversible obstruction of the bronchi and airway inflammation. In recent decades, the scientific interest of the vitamin D system and its role in development of asthma and other alergic diseases has been increased. Aims of this study are to mesure and compare the serum level of 25 OHD in asthmatic and healthy children and corelate the level of 25OHD and total IgE in asthmatic children. This prospective study includes 70 children at age 2 to 14, of which 32 are children with diagnosed asthma and 38 healthy children. In both  of the groups the serum level of 25 OHD was measured  and by the results 18 % of the healthy children (C) and 28% of the asthma children (E) had 25OHD  deficiency, 45%  of C and 50% of E were insufficient and 37 % of C / 22% of E were with normal 25 OHD serum level. The mean level of 25OHD in C was 27,83 +/- 10,24 and in E 20,9 ng/ml +/- 10,72. The mean levels in both groups had statistic significant difference with p-value < 0,05. According to age no statistic significant difference was found in both of the groups. There was a statisticaly significant decreased serum level of 25 OHD in asthmatic females.In the examined group (children with asthma) there was a negative linear correlation (association) of the level of 25OHD and total IgE serum level with r=- 0,55  Vitamin D serum level measurements in asthma patients gives the possibility for discovering the connection between its deficiency and development of asthma symptoms.

scholarly journals The Differentiated Directions of Psychological Correction in Families with Children with Asthma

2017 ◽  
Vol 8 (4) ◽  
pp. 105-110
Author(s):  
Tatyana I. Nikolskaya ◽  
Elena Yu. Galitsyna
Keyword(s):  
Cluster Analysis ◽  
Family Functioning ◽  
Chronic Diseases ◽  
Bronchial Asthma ◽  
Effective Interaction ◽  
Healthy Children ◽  
Children With Asthma ◽  
Current Article ◽  
Families With Children

The current article analyzes the parental defensive styles, codes and types of upbringing in families with children diagnosed with asthma in comparison with families of healthy children. The study involved 80 families. Of these, 41 families with children with asthma (41 mothers and 37 fathers) and 39 families with children without chronic diseases (39 mothers and 38 fathers). It was revealed that the fathers of children with asthma differ in educational insecurity, and mothers have persistent educational stereotypes that interfere with effective interaction with the child. In mothers of children with asthma, non-adaptive protective styles predominate, and fathers distort, whereas in the parents of healthy children adaptive protective styles are expressed. With the cluster analysis, three groups of families with children with asthma are distinguished, differing in the level of effectiveness of family functioning. The conjugation of violations of family functioning and the severity of the asthma in a child was determined. Based on the data obtained, differentiated directions of psycho-correction for families with children with asthma are described, depending on the severity of the child's disease and the level of effectiveness of family functioning. A trial program of psychological correction was conducted, according to the described directions. The analysis of the results of the second study showed the effectiveness of the proposed program of psychological correction for families with children suffering from bronchial asthma.

scholarly journals Metabolites of L-ARG in Exhaled Breath Condensate and Serum Are Not Biomarkers of Bronchial Asthma in Children

2022 ◽  
Vol 11 (1) ◽  
pp. 252
Author(s):  
Joanna Połomska ◽  
Barbara Sozańska
Keyword(s):  
Bronchial Asthma ◽  
Pediatric Patients ◽  
Exhaled Breath Condensate ◽  
Mass Spectrometric ◽  
Mass Spectrometric Analysis ◽  
Spectrometric Analysis ◽  
Exhaled Breath ◽  
Asthmatic Children ◽  
Healthy Control ◽  
Breath Condensate

(1) Background: L-arginine (L-ARG) and its metabolites are involved in some aspects of asthma pathogenesis (airway inflammation, oxidative stress, bronchial responsiveness, collagen deposition). Published data indicate that lungs are a critical organ for the regulation of L-ARG metabolism and that alterations in L-ARG metabolism may be significant for asthma. The aim of this study was to assess the levels of L-ARG and its metabolites in pediatric patients with asthma in serum and exhaled breath condensate (EBC) by mass spectrometric analysis and compare them with non-asthmatic children. (2) Methods: Sixty-five children (37 pediatric patients with bronchial asthma and 28 healthy control subjects) aged 6–17 participated in the study. All participants underwent a clinical visit, lung tests, allergy tests with common aeroallergens, and serum and EBC collection. The levels of biomarkers were determined in both serum and EBC. Analytical chromatography was conducted using an Acquity UPLC system equipped with a cooled autosampler and an Acquity HSS T3 column. Mass spectrometric analysis was conducted using the Xevo G2 QTOF MS with electrospray ionization (ESI) in positive ion mode. (3) Results: Asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) levels in serum and EBC did not differ significantly in asthmatic children and healthy control subjects. We found no correlation between forced expiratory volume in one second (FEV1) and L-ARG and its metabolites, as well as between interleukin-4 (IL-4) serum level and L-ARG and its metabolites. Concentrations of ADMA, SDMA, citrulline (CIT), and ornithine (ORN) were higher in serum than EBC in asthmatics and non-asthmatics. By contrast, concentrations of dimethylarginine (DMA) were higher in EBC than serum. ADMA/L-ARG, SDMA/L-ARG, and DMA/L-ARG ratios were significantly higher in EBC than in serum in asthmatics and in non-asthmatics. (4) Conclusions: Serum and EBC concentrations of L-ARG and its metabolites were not an indicator of pediatric bronchial asthma in our study.

scholarly journals Respiratory function declines in children with asthma associated with chemical species of fine particulate matter (PM2.5) in Nagasaki, Japan

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Yoonhee Kim ◽  
Eun Ha Park ◽  
Chris Fook Sheng Ng ◽  
Yeonseung Chung ◽  
Kunio Hashimoto ◽  
...  
Keyword(s):  
Air Pollution ◽  
Lung Function ◽  
Urban Areas ◽  
Chemical Species ◽  
Mixed Effects ◽  
Linear Mixed Effects Model ◽  
Linear Mixed Effects ◽  
Asthmatic Children ◽  
Transboundary Air Pollution ◽  
Children With Asthma

Abstract Background The differential effects of PM2.5 fractions on children’s lung function remain inconclusive. This study aimed to examine whether lung function in asthmatic children was associated with increased PM2.5 fractions in urban areas in Nagasaki prefecture, Japan, where the air pollution level is relatively low but influenced by transboundary air pollution. Methods We conducted a multiyear panel study of 73 asthmatic children (boys, 60.3%; mean age, 8.2 years) spanning spring 2014–2016 in two cities. We collected self-measured peak expiratory flow (PEF) twice a day and daily time-series data for PM2.5 total mass and its chemical species. We fitted a linear mixed effects model to examine short-term associations between PEF and PM2.5, adjusting for individual and time-varying confounders. A generalized linear mixed effects model was also used to estimate the association for worsening asthma defined by severe PEF decline. Back-trajectory and cluster analyses were used to investigate the long-range transboundary PM2.5 in the study areas. Results We found that morning PEFs were adversely associated with higher levels of sulfate (− 1.61 L/min; 95% CI: − 3.07, − 0.15) in Nagasaki city and organic carbon (OC) (− 1.02 L/min; 95% CI: − 1.94, − 0.09) in Isahaya city, per interquartile range (IQR) increase at lag1. In addition, we observed consistent findings for worsening asthma, with higher odds of severe PEF decline in the morning for sulfate (odds ratio (OR) = 2.31; 95% CI: 1.12, 4.77) and ammonium (OR = 1.73; 95% CI: 1.06, 2.84) in Nagasaki city and OC (OR = 1.51; 95% CI: 1.06, 2.15) in Isahaya city, per IQR increase at lag1. The significant chemical species were higher on days that could be largely attributed to the path of Northeast China origin (for sulfate and ammonium) or both the same path and local sources (for OC) than by other clusters. Conclusions This study provides evidence of the differential effects of PM2.5 fractions on lung function among asthmatic children in urban areas, where the Japanese national standards of air quality have been nearly met. Continuous efforts to promote mitigation actions and public awareness of hazardous transboundary air pollution are needed to protect susceptible children with asthma.

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