scholarly journals Antimicrobial Test of 1-(2.5-Dihydroxi Phenyl)-(3-Pyridine-2-Il) -Propanone Compound in Enterococcus Faecalis and Escherichia Coli Bacteria Using a Well Diffusion Method

2021 ◽  
Vol 1 (2) ◽  
Author(s):  
Andy Eko Wibowo ◽  
Rivaldy Rifai Hatala ◽  
Awalludin M Edang
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Melting Point ◽  
Thin Layer ◽  
Enterococcus Faecalis ◽  
Antibacterial Effect ◽  
Diffusion Method ◽  
Point Test ◽  
Escherichia Coli Bacteria ◽  
Layer Chromatography

1-(2.5-dihydroxy phenyl)-(3-pyridine-2-il)-propenone compound is a compound synthesized by reacting the pyridine-2-carbaldehyde and 2.5-dihydroxyacetophenone compound without solvent with K2CO3 (Potassium Carbonate) catalyst in the microwave. The 1-(2.5-dihydroxy phenyl)-(3-pyridine-2-il)-propenone compound is a chalcone derivative compound substituted by two hydroxy groups on ring A and has 2-pyridyl groups on ring B. Chalcone is a secondary metabolite compound from the flavonoid group, which has several activities as anti-platelet, anti-bacterial, immunomodulator, anti-hyperglycemic, and anti-inflammatory. This study aims to determine the antibacterial effect of 1-(2.5-dihydroxifenil)-(3-pyridine-2-il)-propenone compound against Enterococcus faecalis and Escherichia coli bacteria. This study used TLC (Thin Layer Chromatography) and Melting Point Test to analyze the purity of 1-(2.5-dihydroxy phenyl)-(3-pyridine-2-il)-propenone compound. Meanwhile, the test for antibacterial activity used a well diffusion method. Concentration variation for 1-(2.5-dihydroxifenyl)-(3-pyridine-2-il)-propenone compound as antibacterial in Escherichia coli were 0.25 mg/100 μl, 0.5 mg/200 μl, and 0.75 mg/300 μl. Meanwhile, the concentration variation for Enterococcus faecalis bacteria was 5%, 2.5%, 1.25% and was replicated three times. The results of the compound purity test using the melting point test and Thin Layer Chromatography (TCL) showed that the 1-(2.5-dihydroxy phenyl)-(3-pyridine-2-il)-propenone compound was pure. The results of the antibacterial activity test for 1-(2.5-dihydroxiphenyl)-(3-pyridine-2-il)-propenone compound showed no zone of inhibition at each test concentration. In conclusion, the 1-(2.5-dihydroxifenyl)-(3-pyridine-2-il)-propenone compound did not have an antibacterial effect on Enterococcus faecalis and Escherichia coli bacteria.

scholarly journals AKTIVITAS ANTIBAKTERI DAN ANALISIS KLT-BIOAUTOGRAFI DARI FRAKSI DAUN MENGKUDU (Morinda citrifolia L.)

PHARMACON ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 505
Author(s):  
Aprilia Aslah ◽  
Widya A. Lolo ◽  
Imam Jayanto
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Effect ◽  
Morinda Citrifolia ◽  
Diffusion Method ◽  
Methanol Fraction ◽  
Fractionation Method ◽  
Liquid Fractionation ◽  
Escherichia Coli Bacteria

ABSTRACT Noni is a nutritious plant because it has several ingredients that are important for health. Antibacterial components in noni leaves include glycosides, acubins, saponins and flavonoids. The purpose of this study was to determine the fraction of the extract of noni leaf ethanol whether it has an antibacterial effect and know the class of compounds indentifed as having antibacterial activity after TLC-Bioautografi testing was carried out. The samples were extracted using maceration method with 96% ethanol solvent and fractionation using liquid-liquid fractionation method with methanol, ethyl acetat and n-hexane solvents. Antibacterial activity testing was carried out with concentration variants of 20%, 30% and 40% with agar diffusion method (Kirby and Bauer). The results showed that methanol fraction, ethyl acetat fraction and Noni hexane leaf fraction effectively inhibited Staphylococcus aureus and Escherichia coli bacteria. The largest fraction of inhibiting zone was the concentration of 40% methanol which was categorized as strong. TLC monitoring was carried out using the mobile phase of chloroform : n-hexane (2:1). Antibacterial activity testing carried out by contact bioautography method showed that there were spots on the TLC choromatogram, which produced inhibitory zones. Characteristics of blotches were performed with AlCl3 spotting and it was thought that the spots were flavonoids.  Keywords : Noni, Antibacterial, Staphylococcus aureus, Escherichia coli, TLC-Bioautografi.  ABSTRAK Mengkudu merupakan tanaman yang berkhasiat karena mempunyai beberapa kandungan senyawa yang penting bagi kesehatan tubuh. Komponen yang berkhasiat antibakteri dalam daun mengkudu antara lain adalah glikosida, acubin, saponin dan flavonoid. Tujuan dari penelitian ini adalah untuk mengetahui fraksi dari esktrak etanol daun mengkudu apakah memiliki efek antibakteri dan mengetahui golongan senyawa yang teridentifikasi memiliki aktivitas antibakteri setelah dilakukan pengujian KLT-Bioautografi. Sampel diekstraksi menggunakan metode maserasi dengan pelarut etanol 96% dan fraksinasi dengan metode fraksinasi cair-cair dengan pelarut metanol, etil asetat dan n-heksan. Pengujian aktivitas antibakteri dilakukan dengan varian konsentrasi 20%, 30% dan 40% dengan metode difusi agar (Kirby dan Bauer). Hasil penelitian menunjukan bahwa fraksi metanol, fraksi etil asetat dan fraksi n-heksan daun Mengkudu efektif menghambat bakteri staphylococcus aureus dan Escherichia coli. fraksi yang paling besar zona hambatnya yaitu fraksi metanol konsentrasi 30% yang dikategorikan kuat, dilakukan pemantauan KLT menggunakan fase gerak kloroform : n-heksan (2:1). Pengujian aktivitas antibakteri dilakukan dengan metode bioautografi kontak hasilnya menunjukkan terdapat bercak pada kromatogram KLT yang menghasilkan zona hambat. Karakteristik bercak dilakukan dengan penampak bercak AlCl3 dan diduga bahwa bercak tersebut Flavonoid. Kata kunci : Mengkudu, Antibakteri, Staphylococcus aureus, Escherichia coli , KLT-Bioautografi

scholarly journals ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF BIXIN PIGMENT FROM ANNATTO (Bixa orellana L.) SEEDS

2010 ◽  
Vol 7 (1) ◽  
pp. 88-92 ◽  
Author(s):  
Pipin T. Kurniawati ◽  
H. Soetjipto ◽  
Leenawati Limantara
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Bixa Orellana ◽  
Isolation And Identification ◽  
Double Beam ◽  
Layer Chromatography

Research on Bixa orellana L. have been done to isolate, identify and determine bixin percentage, the antioxidant and antibacterial activities of bixin from B. orellana seed.  Isolation and identification of bixin was done by thin layer chromatography (TLC), column chromatography, chemical test of bixin and UV-Vis double beam spectroscopy. Percentage of bixin was calculated by JECFA method, the antioxidant activity was determined by DPPH (1-1 diphynilpicrylhidrazil) method while antibacterial activity was analyzed by the use of agar diffusion method. Thin layer chromatography (TLC) for the crude extract contained 5 spot, where spot 5th was bixin. Bixa orellana has 75±3% of bixin. Antioxidant activity of bixin had IC50 548.5±20.0 ppm. Whereas the antibacterial activity of bixin against the Escherichia coli and Staphylococus aureus could be classified as weak inhibition category at 500-750 μg and medium inhibition category at 1500 μg.   Keywords: Bixa orellana L., bixin, antioxidant, antibacteria

scholarly journals Synthesis of different α, β- unsaturated oxazolone derivatives

2019 ◽  
Vol 9 (1) ◽  
pp. 124-127
Author(s):  
Pallavi L Phalke
Keyword(s):  
Antibacterial Activity ◽  
Infrared Spectroscopy ◽  
Melting Point ◽  
Acetic Anhydride ◽  
Thin Layer ◽  
Sodium Acetate ◽  
E Coli ◽  
Nucleophilic Reagents ◽  
Formed Product ◽  
Layer Chromatography

Acetyl glycine were prepared from glycine acetic anhydride and then 2, 4-disubstituted Oxazol-5-one were prepared from acetyl glycine, substituted aldehydes, acetic anhydride, and sodium acetate as a catalyst. The formed product is evaluated and characterized by thin layer chromatography, infrared spectroscopy and melting point. The, β- unsaturated shows ability to react with various nucleophilic reagents for synthesis of new fused oxazole compounds. Keywords: glycine, acetic anhydride, aldehyde Sodium acetate, Oxazolone, E. coli, Antibacterial Activity.

scholarly journals UJI AKTIVITAS ANTIBAKTERI DAUN JERUK KALAMANSI (Citrofortunella microcarpa) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli

Alotrop ◽  
2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Deza Oktasila ◽  
Nurhamidah Nurhamidah ◽  
Dewi Handayani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Raw Material ◽  
Diffusion Method ◽  
Citrus Leaves ◽  
Escherichia Coli Bacteria

This study aims to examine the antibacterial activity of ethanol extract and essential oil of Kalamansi citrus leaves (Citrofortunella microcarpa) against Staphylococcus aureus and Escherichia coli bacteria. The raw material of Kalamansi citrus leaves is obtained from the Village of Pondok Kubang, Bengkulu Tengah (3.70060S, 102.35780E). Ethanol extract from Kalamansi citrus leaves was obtained by maceration using ethanol 96%, then ethanol extract was made dilution concentration 40 ; 20; , 10; and 5%. The essential oil of Kalamansi citrus leaves is obtained by water-vapor distillation, then made variations of concentration 20;, 15;, 10;  and 5%. The method used to test the antibacterial activity is the paper disc diffusion method , the antibacterial activity is shown by the diameter of the inhibiting  zone formed. The data of antibacterial test result were analyzed by using One Way Anova test which showed the effect of treatment on the growth of test bacteria seen from the value (P <0,01) and continued by Duncan test to know the effect of the treatment. The results showed that ethanol extract of Kalamansi citrus leaves had antibacterial activity against S. aureus and E. coli bacteria with moderate inhibitory diameter is  7.20 and 5.73 mm at concentration 40%, while antibacterial activity on essential oil of Kalamansi citrus leaves is categorized as strong with inhibition zone diameter is 14.83 and 13.00 mm at concentration 20%.

scholarly journals Antibacterial activity of cis- and trans-resveratrol isolated from Polygonum cuspidatum rhizome

2006 ◽  
pp. 131-136 ◽  
Author(s):  
Zoran Kukric ◽  
Ljiljana Topalic-Trivunovic
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Antibacterial Effect ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Polygonum Cuspidatum ◽  
Disk Diffusion Method ◽  
Cis And Trans

The work is concerned with the antibacterial effect of ethanolic solutions of cis- and trans-resveralrol (cis-.lrans-3,5.4'-trihydroxystilbene) obtained by the extraction (ethanol-water 1:1 v/v) of Polygonum cuspidatum rhizome. Antibacterial activity was tested by disk diffusion method on the following bacteria: Escherichia coli, Sarcina liitea. Bacillus subtilis and Staphylococcits sp., using extract concentrations of 5 mg/disk. All tests showed significant antimicrobial activity, whereby the extract with trans-resveratrol exhibited more significant effect than the extract of cis-resveratrol.

scholarly journals ANTIBACTERIAL ACTIVITY OF ROBUSTA COFFEE (COFFEA CANEPHORA L.) LEAVES TO STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI

2019 ◽  
pp. 113-115
Author(s):  
ZAMHARIRA MUSLIM ◽  
YONANIKO DEPHINTO
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Antibacterial Effect ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Robusta Coffee

Objective: This research aims to analyze the ability of robusta coffee leaves fraction extract to inhibit the growth of Staphylococcus aureus and Escherichia coli and also determine the minimum inhibitory concentration (MIC). Methods: Antibacterial activity evaluated by the disc diffusion method observed in four types of fraction of extract robusta coffee leaves (n-hexane, ethyl acetate, ethanol, and water). Each extract divided into three various concentrations, 5%, 10%, and 15%. Determination of antimicrobial activity in vitro by the disk diffusion method. Results: Ethyl acetate fraction of coffee leaves extract produced the largest diameter zone of inhibition of bacterial growth compared to other extraction fractions of 17.28 mm in E. coli and 18.58 mm in S. aureus. The MIC of coffee leaves extract fraction water, ethyl acetate, and n-hexane on E. coli and S. aureus is 5%, while the fraction ethanol MIC is 10%. Conclusion: The antibacterial effect of ethyl acetate fraction of coffee leaves extract showed an antibacterial effect that was better than the fraction of n-hexane, ethanol, and water.

scholarly journals Antibacterial Activity ofPseudonocardiasp. JB05, a Rare Salty Soil Actinomycete againstStaphylococcus aureus

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Nesa Jafari ◽  
Reza Behroozi ◽  
Davoud Farajzadeh ◽  
Mohammad Farsi ◽  
Kambiz Akbari-Noghabi
Keyword(s):  
Antibacterial Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Scale Up ◽  
Diffusion Method ◽  
Hydroxyl Groups ◽  
Sephadex Column ◽  
Antibacterial Compounds ◽  
Antibacterial Compound ◽  
Layer Chromatography

Staphylococcus aureusis a Gram-positive bacterium that causes many harmful and life-threatening diseases. Some strains of this bacterium are resistant to available antibiotics. This study was designed to evaluate the ability of indigenous actinomycetes to produce antibacterial compounds againstS. aureusand characterize the structure of the resultant antibacterial compounds. Therefore, a slightly modified agar well diffusion method was used to determine the antibacterial activity of actinomycete isolates against the test microorganisms. The bacterial extracts with antibacterial activity were fractionated by silica gel and G-25 sephadex column chromatography. Also, the active fractions were analyzed by thin layer chromatography. Finally, the partial structure of the resultant antibacterial compound was characterized by Fourier transform infrared spectroscopy. One of the isolates, which had a broad spectrum and high antibacterial activity, was designated asPseudonocardiasp. JB05, based on the results of biochemical and 16S rDNA gene sequence analysis. Minimum inhibitory concentration for this bacterium was 40 AU mL−1againstS. aureus. The antibacterial activity of this bacterium was stable after autoclaving, 10% SDS, boiling, and proteinase K. Thin layer chromatography, using anthrone reagent, showed the presence of carbohydrates in the purified antibacterial compound. Finally, FT-IR spectrum of the active compound illustrated hydroxyl groups, hydrocarbon skeleton, and double bond of polygenic compounds in its structure. To the best of our knowledge, this is the first report describing the efficient antibacterial activity by a local strain ofPseudonocardia.The results presented in this work, although at the initial stage in bioactive product characterization, will possibly contribute toward thePseudonocardiascale-up for the production and identification of the antibacterial compounds.

scholarly journals ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI DARI BAKTERI YANG BERASOSIASI DENGAN ALGA Turbinaria ornata (Turner) J. Agardh SERTA IDENTIFIKASI SECARA BIOKIMIA

PHARMACON ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 351
Author(s):  
Cicilia Kosasi ◽  
Widya A. Lolo ◽  
Sri Sudewi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Diffusion Method ◽  
Genus Bacillus ◽  
Biochemical Tests ◽  
Isolation And Purification ◽  
Turbinaria Ornata ◽  
Gram Staining ◽  
Escherichia Coli Bacteria

ABSTRACT Algae are a place to live for various microorganisms’ symbiosis with them, and some of them were known to be used as antibacterial. This study aims to determine the antibacterial activity of associated bacteria isolates from Turbinaria ornata algae obtained from the Bay of Manado against Staphylococcus aureus and Escherichia coli bacteria. In the course of testing the isolation and purification of symbiotic bacteria from Turbinaria ornata algae, then antibacterial activity was testing using agar diffusion method, then gram staining and biochemical tests were carried out including motility test,  tets, carbohydrate fermentation test, citrate test, lysine test, indole test and catalase test. The result showed  that there were 3 bacterial isolates, which had antibacterial activity in medium category against S.aureus and E.coli bacteria. Based on the results of biochemical identification, the isolates that have the greatest inhibitory power, they were thought to belong to genus Bacillus and the genus Yersinia.Keywords : Antibacterial, Symbionic bacteria, Turbinaria ornata (Turner) J.   Agardh. ABSTRAK Alga merupakan tempat hidup berbagai mikroorganisme yang bersimbiosis dengannya, dan beberapa dari spesies alga diketahui sering dimanfaatkan salah satunya  sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antibakteri dari isolat bakteri yang berasosiasi dengan alga Turbinaria ornata yang diperoleh dari Teluk Manado terhadap bakteri  Staphylococcus aureus dan Escherichia coli. Dalam pengujian dilakukan isolasi dan purfifikasi bakteri simbion dari alga Turbinari ornata, kemudian dilakukan pengujian aktivitas antibakteri menggunakan metode difusi agar, selanjutnya silakukan pewarnaan gram dan uji biokimia meliputi uji motilitas, uji H2S, uji fermentasi karbohidrat, uji sitrat, uji lisin, uji indol dan uji katalase. Hasil penelitian menunjukkan bahwa terdapat 3 isolat bakteri yang mempunyai daya aktivitas antibakteri dalam kategori sedang terhadap bakteri S. aureus dan E. coli. Berdasarkan hasil identifikasi secara biokimia isolat yang mempunyai daya hambat paling besar diduga termasuk dalam genus Bacillus dan genus Yersinia. Kata kunci : Antibakteri, Bakteri simbion, Turbinaria ornata (Turner) J. Agardh.

scholarly journals Fitokimia Dan Aktivitas Antibakteri Dari Daun KAF(Chisocheton sp. (C. DC) HARMS)

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 42
Author(s):  
Florentin Natalia Melsadalam ◽  
Dewa Gede Katja ◽  
Meiske S. Sangi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Inhibitory Zone ◽  
Escherichia Coli Bacteria

Telah dilakukan penelitian yang bertujuan untuk mengidentifikasi senyawa metabolit sekunder  dari ekstrak daun kaf menggunakan tiga jenis pelarut yakni n-heksan, etil asetat, dan metanol. Ekstrak yang diperoleh diuji aktivitas antibakterinya dengan metode difusi yang menggunakan bakteri Staphylococcusaureus dan Escherichia coli. Dari hasil pengujian fitokimia, ketiga ekstrak tersebut menunjukan bahwa sampel mengandung senyawa; alkaloid, saponin, steroid, flavonoid dan tanin. Pengujian aktivitas antibakteri  dengan metode difusi menunjukan hasil yang paling tinggi aktivitas antibakteri adalah  estrak etil asetat dengan zona hambat 35,75 mm terhadap bakteri  Staphylococcus aureus, sedangkan pada ekstrak metanol memiliki zona hambat 23,75 mm terhadap bakteri Escherichia coliResearch has been carried out aimed at identifying secondary metabolites of kaf leaf extract using three types of solvents, namely n-hexane, ethyl acetate, and methanol.The obtained extracts were tested for antibacterial activity by diffusion method using Staphylococcus aureus and Escherichia coli bacteria. From the results of phytochemical testing, the three extracts showed that the sample contained compounds; alkaloids, saponins, steroids, flavonoids and tannins. Antibacterial activity testing with diffusion method showed the highest antibacterial activity was estracethyl ethyl acetate with 35.75 mm inhibition zone against Staphylococcus aureus bacteria, whereas methanol had 23.75 mm inhibitory zone against Escherichia coli bacteria

scholarly journals UJI EFEK ANTIBAKTERI JAMUR ENDOFIT PADA TUMBUHAN SEREH (Cymbopogon citratus) PADA BAKTERI UJI Staphylococcus aureus DAN Escherichia coli

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 705
Author(s):  
Fathia Falugah ◽  
Jimmy Posangi ◽  
Paulina V. Y. Yamlean
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Pathogenic Bacteria ◽  
Antibacterial Effect ◽  
Cymbopogon Citratus ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Escherichia Coli Bacteria

ABSTRACT Endophytic fungi are fungi that live in plant tissues and do not harm these plants. Endophytic fungi can produce compounds that have the potential as antibacterial. This study aimed to examine the antibacterial effects of endophytic fungi isolated from the lemongrass (Cymbopogon citratus) against Staphylococcus aureus and Escherichia coli bacteria. Lemongrass is used to inhibit or kill pathogenic bacteria because it contains essential oils that function as an antifungal and antibacterial against several pathogenic bacteria such as Staphylococcus aureus and Escherichia coli. The laboratory experiment method tested the antibacterial activity of endophytic fungi isolates obtained from lemongrass (Cymbopogon citratus) against Staphylococcus aureus and Escherichia coli using hole / well method. From the results of the study, four endophytic fungi were isolated from the roots and leaves of lemongrass (Cymbopogon citratus). Antibacterial results showed that endophytic fungi extract isolated from lemongrass plants was more effective in inhibiting Staphylococcus aureus compared to Escherichia coli bacteria. In Staphylococcus aureus bacteria the average inhibitory value is 5-9 mm while in Escherichia coli bacteria the average inhibitory value is only 3 mm. Endophytic fungi from lemongrass roots have a better antibacterial effect compared to endophytic fungi from lemongrass leaves. Conclusion, endophytic fungi isolated from the roots and leaves of lemongrass (Cymbopogon citratus) have an antibacterial effect on the growth of S. aureus bacteria and are not effective against E. coli bacteria. This endophytic fungus isolate is more active against Gram positive (+) bacteria and less active against Gram negative (-) bacteria. Keywords: Lemongrass plants, Endophytic Fungi, Antibacterial Activity, Staphylococcus aureus,  Escherichia coli. ABSTRAK Jamur endofit ialah jamur yang hidup di dalam jaringan tumbuhan dan tidak membahayakan tumbuhan tersebut. Jamur endofit dapat menghasilkan senyawa yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk menguji efek antibakteri jamur endofit yang diisolasi dari akar,batang dan daun tumbuhan sereh (Cymbopogon citratus)  terhadap bakteri Staphylococcus aureus dan Escherichia coli. Sereh digunakan untuk menghambat atau membunuh bakteri patogen karena mengandung minyak atsiri yang berfungsi sebagai antijamur dan antibakteri terhadap beberapa bakteri patogen seperti Staphylococcus aureus dan  Escherichia coli.  Metode penelitian secara eksperimen laboratorium menguji aktivitas antibakteri dari isolat jamur endofit  yang diperoleh dari tumbuhan sereh (Cymbopogon citratus) pada bakteri Staphylococcus Aureus dan Escherichia Coli menggunakan metode lubang/sumuran. Dari hasil penelitan diperoleh empat jenis jamur endofit yang diisolasi dari akar dan daun  tumbuhan sereh (Cymbopogon citratus).  Hasil penelitian antibakteri menunjukkan bahwa ekstrak jamur endofit yang diisolasi dari tumbuhan sereh lebih efektif menghambat  bakteri Staphylococcus aureus dibandingkan dengan bakteri Escherichia coli. Pada bakteri  Staphylococcus aureus nilai rata-rata daya hambat yaitu 5-9 mm sedangkan pada bakteri Escherichia coli nilai rata-rata daya hambat yaitu hanya 3 mm. Jamur endofit dari akar sereh memiliki efek antibakteri yang lebih baik dibandingkan dengan jamur endofit dari daun sereh. Kesimpulan, Jamur endofit yang diisolasi dari akar  dan daun sereh (Cymbopogon citratus) memiliki efek antibakteri terhadap pertumbuhan bakteri S. aureus dan tidak efektiv terhadap bakteri E. coli. Isolat jamur endofit ini lebih aktif pada bakteri Gram positif (+) dan kurang  aktif pada bakteri negatif (-). Kata Kunci : Tumbuhan Sereh, Jamur Endofit, Aktivitas Antibakteri, Staphylococcus aureus, Escherichia coli

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