scholarly journals Evaluation of reproductive profile in male albino rats following varied duration of administration with Revive capsule

2021 ◽  
Vol 10 (8) ◽  
pp. 2981
Author(s):  
Onengiyeofori Ibama ◽  
Kinikanwo I. Green ◽  
Edna O. Nwachuku ◽  
Adline Erinma Ben-Chioma
Keyword(s):  
Erectile Dysfunction ◽  
Sperm Quality ◽  
Sperm Count ◽  
Negative Control ◽  
Albino Rats ◽  
Once Daily ◽  
Treatment Groups ◽  
Human Dose ◽  
Serum Lh ◽  
Varied Duration

Background: Revive capsule is a polyherbal formulation commonly used to treat erectile dysfunction or enhance libido in men. Some of the individual herbs used in the formulation of this drug have been known scientifically to affect various biochemical components of the human body; hence this study was aimed at evaluating the reproductive profile in male albino rats following varied duration of administration with Revive capsule.  Methods: A total of 42 male albino rats were used for the study, and were divided into six (6) groups of seven (7) rats each. They were allowed to acclimatize for two (2) weeks by maintaining 12-hour light and dark cycles daily, with access to standard feed and water ad libitum. Group A (negative control) rats were administered with distilled water once daily, while groups B, C, D, E and F were administered once daily with 72 mg/kg of Revive capsule for 1 week, 2 weeks, 3 weeks, 4 weeks and 6 weeks respectively. The rat dose administered was extrapolated from the human dose using the formula by Paget and Barnes. At the end of each treatment week, the rats were allowed to fast overnight, followed by their anaesthetization using chloroform, and blood sample collection via jugular vein puncture. Also, the testes were excised; the epididymis were also excised from the testes and used immediately for semen analysis, while the epididymis-free testes were examined histologically. Rat-specific test kits with ELISA method were used to analyze serum LH, FSH and testosterone.Results: The results showed a significant increase (p<0.05) in serum LH, FSH and testosterone levels, and a significant increase in sperm count and sperm quality parameters in the treatment groups compared to the negative control, with the maximum levels attained after 6 weeks of treatment (group F). Also, photomicrographs of histologically examined testes of the treatment groups appeared indifferent from those of the negative control.Conclusions: These findings may suggest that in using a rat model, treatment with Revive capsule at the appropriate dosage for 6 weeks is safe, and that, besides its acclaimed use in enhancing libido or treating erectile dysfunction, it may also be effective in promoting male fertility. 

Amelioration of sperm count and sperm quality by lycopene supplementation in irradiated mice

2020 ◽  
Vol 32 (12) ◽  
pp. 1040
Author(s):  
Małgorzata M. Dobrzyńska ◽  
Aneta Gajowik
Keyword(s):  
Dna Damage ◽  
Sperm Quality ◽  
Sperm Count ◽  
Combined Treatment ◽  
Treatment Groups ◽  
Patients With Cancer ◽  
Equivalent Time ◽  
Radiation Induced ◽  
Almost All ◽  
Harmful Effects

Male mice were exposed to lycopene (LYC; 0.15 and 0.30mg kg−1) and irradiation (0.5, 1 Gy) alone or in combination (0.5 Gy+0.15mg kg−1 LYC; 0.5 Gy+0.30mg kg−1 LYC; 1 Gy+0.15mg kg−1 LYC; 1 Gy+0.30mg kg−1 LYC) for 2 weeks. LYC administration in the drinking water was started 24h or on Day 8 after the first irradiation dose or equivalent time point for groups treated with LYC alone. Sperm count, motility, morphology and DNA damage were determined at the end of the 2-week treatment period. Irradiation deteriorated sperm count and quality. Supplementation with LYC from 24h significantly increased the sperm count compared with irradiation alone. In almost all combined treatment groups, the percentage of abnormal spermatozoa was significantly decreased compared with that after irradiation alone. In some cases, combined treatment reduced levels of DNA damage in gametes. Both doses of LYC administered from Day 8 significantly reduced the percentage of morphologically abnormal spermatozoa compared with that seen after 1 Gy irradiation and reduced DNA damage in all combined treatment groups. In conclusion, LYC supplementation after irradiation can ameliorate the harmful effects of irradiation on gametes. Mitigation of radiation-induced damage in germ cells following LYC administration may be useful for radiological accidents and to protect non-treated tissues in patients with cancer undergoing radiotherapy.

scholarly journals The Effect of Eurycoma longifolia on Sperm Quality of Male Rats

2009 ◽  
Vol 4 (10) ◽  
pp. 1934578X0900401 ◽  
Author(s):  
Kit-Lam Chan ◽  
Bin-Seng Low ◽  
Chin-Hoe Teh ◽  
Prashanta K. Das
Keyword(s):  
Testosterone Level ◽  
Sperm Quality ◽  
Sperm Count ◽  
Male Rats ◽  
Plasma Testosterone ◽  
Seminiferous Tubules ◽  
Albino Rats ◽  
Meoh Extract ◽  
Plasma Testosterone Level ◽  
Etoac Fraction

The present study investigated the effects of a standardized methanol extract of E. longifolia Jack containing the major quassinoid constituents of 13α(21)-epoxyeurycomanone (1), eurycomanone (2), 13α,21-dihydroeurycomanone (3) and eurycomanol (4) on the epididymal spermatozoa profile of normal and Andrographis paniculata induced infertile rats. The standardized MeOH extract at doses of 50, 100 and 200 mg/kg, the EtOAc fraction (70 mg/kg), and standardized MeOH extract at 200 mg/kg co-administered with the EtOAc fraction of A. paniculata at 70 mg/kg were each given orally to male Sprague-Dawley albino rats for 48 consecutive days. The spermatozoa count, morphology, motility, plasma testosterone level and Leydig cell count of the animals were statistically analyzed by ANOVA with a post-hoc Tukey HSD test. The results showed that the sperm count of rats given the standardized MeOH extract alone at doses of 50, 100 and 200 mg/kg were increased by 78.9, 94.3 and 99.2 %, respectively when compared with that of control (p < 0.01). The low count, poor motility and abnormal morphology of the spermatozoa induced by the A. paniculata fraction were significantly reversed by the standardized MeOH extract of E. longifolia (p < 0.001). The plasma testosterone level of the rats treated with the standardized MeOH extract at 200 mg/kg was significantly increased (p < 0.01) when compared with that of the control and infertile animals. The spermatocytes in the seminiferous tubules and the Leydig cells appeared normal. Testosterone level was significantly higher in the testes (p < 0.01) than in the plasma after 30 days of oral treatment with the standardized MeOH extract. Interestingly, eurycomanone (2) alone was detected in the rat testis homogenates by HPLC-UV and confirmed by LC/MS, and may have contributed towards the improvement of sperm quality. Thus, the plant may potentially be suitable for the management of male infertility.

scholarly journals Effect of Phyllanthus amarus on Some Reproductive Indices of Male Albino Rats

2019 ◽  
pp. 1-8
Author(s):  
P. B. Ekpo ◽  
N. E. Edu ◽  
A. J. Umoyen ◽  
T. L. Thomas ◽  
S. O. Abraham
Keyword(s):  
Medicinal Plants ◽  
Sperm Count ◽  
Reproductive Organs ◽  
Male Rats ◽  
Albino Rats ◽  
Phyllanthus Amarus ◽  
Experimental Animals ◽  
Treatment Groups ◽  
Randomized Design ◽  
Group B

Background: Medicinal plants have been a good source of drugs for humans, but chronic and prolong use of medicinal plants like Phyllanthus amarus for the treatment of malaria and other disorders are issues of concerns. This study evaluated the effect of Phyllanthus amarus on reproductive organs and sperm parameters in albino rats. Materials and Methods: Twenty-four healthy male albino rats of 12 weeks old were assigned into four groups with six rats in each group using a Completely Randomized Design (CRD). The experimental animals were orally treated with Phyllanthus amarus. Group A served as the control and was given only water and feed; Group B, C and D  received 100 mg/kgBW, 200 mg/kgBW and 300 mg/kgBW of Phyllanthus amarus respectively. Data obtained were analyzed using analysis of variance (ANOVA). The treatments lasted for a period of 65 days after two weeks of acclimatization. Results: The results showed statistically significant (p<0.05) reduction in weight of testes and epididymes, sperm motility, sperm viability, sperm count and sperm head abnormalities in male  rats treated with Phyllanthus amarus when compared to the control. The sperm pH was not significantly (p>0.05) affected by Phyllanthus amarus among the different treatment groups in the experimental animals. Conclusion: Findings from the present study indicate that Phyllanthus amarus possesses a dose-dependent anti-fertility activity in amale albino rats under a sub-chronic course of administration.

scholarly journals Aphrodisiac Effects of Areca Fruit in Erectile Dysfunction Rat Model

2019 ◽  
Vol 3 (3) ◽  
pp. 16-23
Author(s):  
Nita Parisa ◽  
Muhammad Totong Kamaluddin ◽  
Salni Salni
Keyword(s):  
Erectile Dysfunction ◽  
Negative Control ◽  
Control Group ◽  
Albino Rats ◽  
Female Rat ◽  
Control Group Design ◽  
Post Test ◽  
Animal House ◽  
Test Control

Abstract. Erectile Dysfunction (ED) cases are quite high in both women and men and increase with age. Areca fruit, ginseng and purwoceng are plants that have been traditionally used to increase male vitality. This in vivo experimental research study with pre and post-test control group design located in the laboratory of animal house and the biomolecular laboratory Faculty of Medicine Sriwijaya University. The subjects of this study were albino rats (Rattus norvegicus), Wistar strains, divided into 6 groups are negative control, Sildenafil, Areca Fruit Extract (AFE) 50 mg/kgBW, AFE 100 mg/kgBW, Combination of Areca Fruit, Ginseng and Purwoceng (AGP) 50 mg/kgBW and AGP 100 mg/kgBW. Female rat was injected by giving 500 mg/100 g of subcutaneous progesterone and 10 μg/100 g of BB benzoate 48 hours before mating so that female mice enter the esterus phase. In this study it was found that Combination of Areca fruit, Ginseng and Purwoceng (AGP) group 50mg/kgBW significantly improved testosterone levels compared to sildenafil, while single areca fruit (AFE) dose 50mg/kgBW increased FSH and LH levels as well as sildenafil statistically.   Keywords: areca fruit, erectile dysfunction, FSH, ginseng, LH, purwoceng, testosteron.

Melatonin and vitamin C exacerbate Cannabis sativa-induced testicular damage when administered separately but ameliorate it when combined in rats

2016 ◽  
Vol 27 (3) ◽  
Author(s):  
Isiaka A. Alagbonsi ◽  
Luqman A. Olayaki ◽  
Toyin M. Salman
Keyword(s):  
Vitamin C ◽  
Cannabis Sativa ◽  
Sperm Count ◽  
Oral Treatment ◽  
Weight Ratio ◽  
Albino Rats ◽  
Lactate Dehydrogenase Activity ◽  
Treatment Groups ◽  
Group I ◽  
Total Antioxidant

AbstractThe mechanisms involved in the spermatotoxic effect ofFifty-five (55) male albino rats (250–300 g) were randomly divided in a blinded fashion into five oral treatment groups as follows: group I (control, n=5) received 1 mL/kg of 10% ethanol for 30 days; groups IIa, IIb, and IIc (n=5 each) received 2 mg/kgCannabis treatments reduced the Johnsen score, sperm count, motility, morphology, paired testicular/body weight ratio, and total antioxidant capacity, but increased lactate dehydrogenase activity. In addition, supplementation of cannabis-treated rats with either melatonin or vitamin C exacerbates the effect of cannabis on those parameters, whereas combination of melatonin and vitamin C reversed the trend to the level comparable to control.This study further showed the gonadotoxic effect of

scholarly journals KOPI DAPAT MENURUNKAN AKTIVITAS ALT DAN AST SERUM DARAH TIKUS WISTAR ALKOHOLIK

2018 ◽  
Vol 6 (2) ◽  
pp. 56
Author(s):  
I Made Sara Wijana ◽  
I Gede Suranjaya ◽  
Iriani Setyawati
Keyword(s):  
Body Weight ◽  
Data Analysis ◽  
Correlation Analysis ◽  
Blood Serum ◽  
Correlation Coefficient ◽  
Negative Control ◽  
Albino Rats ◽  
Treatment Groups ◽  
Wistar Albino Rats ◽  
Alt Activity

Ethanol can damage the liver, indicated by an increase of alanin amino transaminase (ALT) activity and aspartat amino transminase (AST) activity in the blood of alcohol-treated rats. Coffee is rich in antioxidants which are hepatoprotector agent. This study aimed to determine the effect of coffee to reduce alanin amino transaminase (ALT) activity and aspartat amino transminase (AST) activity in blood serum of alcohol-treated rats. The study used 32 male wistar albino rats (200±20g  weight) which were divided into four treatment groups i.e. P0 (negative control which were treated with 1 mL of 30% ethanol/200g body weight (bw)/day), P1 (1 mL of 30% ethanol/200 g bw/day + coffee 0,1g/200g bw in 3,5 mL of water/200 g bw/day), P2 (1 mL of 30% ethanol/200g bw + coffee 0,15 g/200g bw in 3,5 mL of water/200g bw/day), dan P3 (1 mL of 30% ethanol/200g bw + coffee 0,2g/200g bw in 3,5 mL of water/200g bw/day). ALT and AST levels were measured by spectrophotometric method. Data analysis was done by ANOVA followed with LSD test and correlation analysis. The results showed that coffee reduced (P<0.01) the activities of ALT and AST of rats blood serum. ALT and AST activities were significantly different (P<0.01) among treatments and with control. Dose of coffee was negatively correlated with ALT activity with correlation coefficient (R) -0.978 and AST with correlation coefficient (R) -0.985 (P<0,01). The lowest of ALT and AST activities were found at dose of coffee 0.2 g/200g body weight. Keywords: ethanol, ALT, AST, coffee  

scholarly journals Effect of vitamin C on testosterone level, sperm count and sperm morphology in gentamicin-induced Wistar rats

2019 ◽  
Vol 7 (2) ◽  
pp. 451 ◽  
Author(s):  
Iis Rahayu ◽  
Elly Usman ◽  
Mohamad Reza
Keyword(s):  
Vitamin C ◽  
Wistar Rats ◽  
Sperm Morphology ◽  
Sperm Quality ◽  
Sperm Count ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
Control Group Design ◽  
Testicular Cells

Background: Gentamicin is an aminoglycoside-class antibiotic that can lead to an increase in ROS and decrease antioxidant reserves that cause destruction of testicular cells that are known to inhibit cell division in testicular germ cells and protein synthesis in the testes as well as affect the production of testosterone that affects the quality of spermatogenesis. Vitamin C as an antioxidant can counteract ROS so that testicular cells can be repaired.Methods: This study was an experimental research with post tests only control group design on 25 male rats, 2-3 aged months, weight 160-240 g. Animals were divided into 5 groups. Negative control group was placed in cage without treatment, positive control given induced gentamicin 5mg/kgbb for the first 10 days, and 3 treatment given induced gentamicin 5mg/kgbb for the first 10 days and at days 11-51 were given vitamin C at a dose of 1.6mg; 2.25mg; 4.5mg. On 52 day, all of wistar rats perform terminated for analyzed the testosterone levels were by ELISA, sperm count was calculated with then haemocytometer observed with digital microscope, morphology of spermatozoa was analyzed by eosin staining and was observed with digital microscope.Results: Results of this study showed that the average of testosteron serum on control negatif group of 3,82ng/ml, control positif 3.73ng/ml, treatment 1 is 9.52ng/ml, treatment 2 is 8.29ng/ml, and traetment 3 is 2.28ng/ml. Sperm count on control negatif 41.68 million/ml, control positif 21.06million/ml, treatment 1 is 52.92 million/ml, treatment 2 is 57.12million/ml, treatment 3 is 80.88 million/ml and sperm morphology on control negative  74.00%, control positif 46.96%, treatment 1 is 58.82%, treatment 2 is 68.68%, and treatment 3 is 78.72%.Conclusions: It is concluded that vitamin C has significant effect on increasing testosteron level and sperm quality in gentamicin-induced wistar rats.

scholarly journals Improvement of Lead Acetate-Induced Testicular Injury and Sperm Quality Deterioration by Solanum Anomalum Thonn. Ex. Schumach Fruit Extracts in Albino Rats

2019 ◽  
Author(s):  
Samuel James Offor ◽  
Herbert Orji Mbagwu ◽  
Orish Ebere Orisakwe
Keyword(s):  
Lead Acetate ◽  
Sperm Quality ◽  
Sperm Count ◽  
Treated Group ◽  
Protective Role ◽  
Albino Rats ◽  
Acetate Solution ◽  
Group 4 ◽  
Group 2 ◽  
Testicular Injury

Objective: This study has investigated the protective role of a natural alternative, Solanum anomalum fruit extract in lead induced testicular toxicity in male albino rats. Materials and methods: Twenty-four mature male albino rats were used, divided into four groups of six rats per group. Group 1 (control rats) were given distilled water (10ml/kg), group 2 received lead acetate solution 60mg/kg, group 3 received lead acetate (60mg/kg) followed by Solanum anomalum (452mg/kg) and group 4 rats were given lead acetate (60mg/kg) followed by Solanum anomalum (678mg/kg) by oral gavage daily for 28 days. Results: Lead treated group showed significant increase in Malondialdehyde MDA (1.58 ± 0.09 to 1.90 ± 0.17 µmol/L of plasma) and decreases in Superoxide dismutase SOD, glutathione peroxidase (482.85 ± 53.43 to 247.18 ± 70.40 U/L of blood), total glutathione (1.11 ± 0.03 to 0.56 ± 0.31 ng/µL) (144.80 ± 7.00 and 122.39 ± 4.63 U/ml of blood), epididymal sperm reserve, testicular sperm count, % sperm motility and % sperm viability. Conclusion: Co-administration of Solanum anomalum significantly reversed the effect of lead with restoration of histoarchitecture of the testes. Solanum anomalum may be a protective modulator of lead -induced testicular injury.

scholarly journals The effect of sesame intake on adult albino rat testis during sildenafil long-term administration

2021 ◽  
Vol 82 (1) ◽  
Author(s):  
M. Goweder Fawzia ◽  
T. M. Abu El-Nasr ◽  
A. A. El-Sayed
Keyword(s):  
Erectile Dysfunction ◽  
Sperm Count ◽  
Chronic Administration ◽  
Prolonged Treatment ◽  
Seminiferous Tubules ◽  
Albino Rats ◽  
Albino Rat ◽  
Term Administration

Abstract Background Sildenafil (Viagra) is widely used for the treatment of erectile dysfunction under various etiologies. Unfortunately, chronic administration of sildenafil negatively affects fertility. The aim of the present study is to investigate the improvement role of sesame on the testis alterations caused by long-term administration of sildenafil on albino rats. Results Light microscopically observations showed that overdoses of sildenafil had provoked tubular and interstitial histological alterations. Tubular degeneration and abnormality in the germinal epithelium of the seminiferous tubules included spermatocyte degeneration and arrest of spermatogenesis. Sperm count and motility were negatively affected by sildenafil. These alterations were improved by sesame feeding. Conclusion Sesame intake improve the side effects of prolonged treatment with sildenafil significantly. These results demonstrate the pivotal role of sesame that may improve male infertility.

The Impact of Gemcitabine-induced Reproductive Toxicity on The Sperm count and Morphology of Albino Rats

2021 ◽  
Vol 12 (1) ◽  
pp. 357-365
Author(s):  
Bassam Abdulaziz Alahmadi
Keyword(s):  
Reproductive System ◽  
Sperm Count ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Human Dose ◽  
Significant Difference ◽  
Recovery Group ◽  
Sperm Count And Morphology ◽  
The Impact

Cancer treatments can affect sperm production and a significant percentage of cancer patients may develop permanent azoospermia or severe oligozoospermia after chemotherapy. To investigate the influence of Gemcitabine toxicity on the reproductive system of albino male rats (sperm count and morphology). An experimental animal study conducted in the zoology department, College of Science, King Saud University during the period from June to October 2014 using albino rats (Rattusnorvegicus) (Wistar strain). Males were divided into four different groups (control" 0 mg/kg",7 mg/kg,14 mg/kg, and 21 mg/kg). The reproductive organs, testicles and epididymis decreased in weight and atrophied in most of the animals treated with the drug in various doses. The mean absolute and relative epididymal weights were also significantly decreased. In the drug-effects recovery group, neither the testicles nor the epididymis in the animals treated with the three doses recovered fully normal weight. The testis's efficiency in producing sperm was significantly decreased at all doses. In the recovery group, the testis regained its efficiency, as no significant difference was recorded between the drug-treated groups and the control group. The drug caused complete loss of sperm, in a rat treated with the big dose. Gemcitabine caused a significant increase in the percentage of deformed sperms in all treated animals. Gemcitabine drug has high toxicity on the reproductive system of rats with a dose tenth of human dose, with a massive decrease in the count and quantity of sperm, which means that this drug can have more toxicity effects on human.

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